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host genotype and environment affect the trade-off between horizontal and vertical transmission of the parasite edhazardia aedis.if a parasite is able to transmit horizontally or vertically, which transmission mode will it choose? we investigated how the growth conditions and the genotype of the mosquito aedes aegypti affect the transmission mode of the parasite edhazardia aedis.201829699504
structural characterization of bacillus licheniformis dahb1 exopolysaccharide-antimicrobial potential and larvicidal activity on malaria and zika virus mosquito vectors.microbial polysaccharides produced by marine species play a key role in food and cosmetic industry, as they are nontoxic and biodegradable polymers. this investigation reports the isolation of exopolysaccharide from bacillus licheniformis dahb1 and its biomedical applications. bacillus licheniformis dahb1 exopolysaccharide (bl-eps) was extracted using the ethanol precipitation method and structurally characterized. ftir and 1h-nmr pointed out the presence of various functional groups and primary ...201829704178
[applications of the crispr/cas9 system in insects].the crispr/cas9 (clustered regularly interspaced short palindromic repeat/crispr-associated) system guides cas9 to specific genomic locations by a short rna search string. this technology enables the systematic interrogation of mammalian genome editing, repairing damaged genes, silencing harmful genes and improving quality traits. in recent years, with the introduction of the crispr/cas9 system for easy, fast and efficient genetic modification, it has been possible to conduct meaningful function ...201829704373
identification of candidate iron transporters from the zip/znt gene families in the mosquito aedes aegypti.mosquito-transmitted viral pathogens, such as dengue and zika, afflict tens of thousands of people every year. these viruses are transmitted during the blood-feeding process that is required for mosquito reproduction, the most important vector being aedes aegypti. while vertebrate blood is rich in protein, its high iron content is potentially toxic to mosquitoes. although iron transport and sequestration are essential in the reproduction of vector mosquitoes, we discovered that culicine mosquito ...201829706902
parallel evolution of vgsc mutations at domains is6, iis6 and iiis6 in pyrethroid resistant aedes aegypti from mexico.aedes aegypti is the primary urban mosquito vector of viruses causing dengue, zika and chikungunya fevers -for which vaccines and effective pharmaceuticals are still lacking. current strategies to suppress arbovirus outbreaks include removal of larval-breeding sites and insecticide treatment of larval and adult populations. insecticidal control of ae. aegypti is challenging, due to a recent rapid global increase in knockdown-resistance (kdr) to pyrethroid insecticides. widespread, heavy use of p ...201829712956
efficacy of larvicides for the control of dengue, zika, and chikungunya vectors in an urban cemetery in southern mexico.many countries in latin america have recently experienced outbreaks of zika and chikungunya fever, in additional to the usual burden imposed by dengue, all of which are transmitted by aedes aegypti in this region. to identify potential larvicides, we determined the toxicity of eight modern insecticides to a. aegypti larvae from a colony that originated from field-collected insects in southern mexico. the most toxic compounds were pyriproxyfen (which prevented adult emergence) and λ-cyhalothrin, ...201829713901
dehydration prompts increased activity and blood feeding by mosquitoes.current insights into the mosquito dehydration response rely on studies that examine specific responses but ultimately fail to provide an encompassing view of mosquito biology. here, we examined underlying changes in the biology of mosquitoes associated with dehydration. specifically, we show that dehydration increases blood feeding in the northern house mosquito, culex pipiens, which was the result of both higher activity and a greater tendency to land on a host. similar observations were noted ...201829717151
high throughput discovery of protein variants using proteomics informed by transcriptomics.proteomics informed by transcriptomics (pit), in which proteomic ms/ms spectra are searched against open reading frames derived from de novo assembled transcripts, can reveal previously unknown translated genomic elements (tges). however, determining which tges are truly novel, which are variants of known proteins, and which are simply artefacts of poor sequence assembly, is challenging. we have designed and implemented an automated solution that classifies putative tges by comparing to referenc ...201829718325
vector competence of certain culex and aedes mosquitoes for the chittoor virus, the indian variant of the batai virus.chittoor virus (chitv), a mosquito-borne bunyavirus (orthobunyavirus: bunyaviridae) isolated in india, has been found to be antigenically close to the batai virus (batv), which has a wide distribution across asia, europe, and africa. the latter virus causes influenza-like illness in humans and mild illness in sheep and goats. batv has been involved in genetic reassortment with other bunyaviruses, generating novel genome combinations and causing severe clinical manifestations including hemorrhagi ...201829718685
wolbachia significantly impacts the vector competence of aedes aegypti for mayaro virus.wolbachia, an intracellular endosymbiont present in up to 70% of all insect species, has been suggested as a sustainable strategy for the control of arboviruses such as dengue, zika and chikungunya. as mayaro virus outbreaks have also been reported in latin american countries, the objective of this study was to evaluate the vector competence of brazilian field-collected ae. aegypti and the impact of wolbachia (wmel strain) upon this virus. our in vitro studies with aag2 cells showed that mayaro ...201829720714
contributions from the silent majority dominate dengue virus transmission.despite estimates that, each year, as many as 300 million dengue virus (denv) infections result in either no perceptible symptoms (asymptomatic) or symptoms that are sufficiently mild to go undetected by surveillance systems (inapparent), it has been assumed that these infections contribute little to onward transmission. however, recent blood-feeding experiments with aedes aegypti mosquitoes showed that people with asymptomatic and pre-symptomatic denv infections are capable of infecting mosquit ...201829723307
larval density mediates knockdown resistance to pyrethroid insecticides in adult aedes aegypti.understanding mechanisms driving insecticide resistance in vector populations remains a public health priority. to date, most research has focused on the genetic mechanisms underpinning resistance, yet it is unclear what role environmental drivers may play in shaping phenotypic expression. one of the key environmental drivers of aedes aegypti mosquito population dynamics is resource-driven intraspecific competition at the larval stage. we experimentally investigated the role of density-dependent ...201829724237
advising a woman with suspected zika virus infection.the zika virus (zikv) epidemic is spreading: 67 countries are now reporting transmission, and over 2,000 cases of congenital zika syndrome (czs) have been confirmed. the heaviest burden has been borne by those living where poverty, poor infrastructure, and lack of access to health services are common and the penetration of aedes aegypti is high. because most cases are asymptomatic, the most dramatic signs of the disease appear through the czs cases. in spite of the need for disaggregated epidemi ...201829724698
improving the delivery and efficiency of fungus-impregnated cloths for control of adult aedes aegypti using a synthetic attractive lure.entomopathogenic fungi are highly promising agents for controlling aedes aegypti mosquitoes. deploying fungus-impregnated black cloths in pet traps efficiently reduced ae. aegypti female survival rates under intra-domicile conditions. with the aim of further increasing the effectiveness of the traps, the addition of attractive lures to fungus-impregnated traps was evaluated.201829728121
male contributions during mating increase female survival in the disease vector mosquito aedes aegypti.aedes aegypti is a vector of medically important viruses including those causing zika, dengue, and chikungunya. during mating, males transfer a number of proteins and other molecules to the female and these components of the male ejaculate are essential in shifting female post-mating behaviors in a number of insect species. because these molecules are highly variable by species, and female post-mating behavior by species is also varied, behavioral assays testing the function of the ejaculate are ...201829729859
antibodies against chikungunya in northern mozambique during dengue outbreak, 2014.an outbreak of dengue and high densities of aedes aegypti were reported in 2014 in northern mozambique, suggesting an increased risk for other arboviruses such as chikungunya virus (chikv) in this region. the aim of this study was to investigate the occurrence of chikv during an outbreak of dengue virus (denv) in pemba city in northern mozambique in 2014. febrile patients (n = 146) seeking medical attention at the pemba provincial hospital between march and april 2014 were enrolled in this study ...201829733254
crystal structure of a novel kunitz type inhibitor, alocasin with anti-aedes aegypti activity targeting midgut proteases.the pesticidal properties of many kunitz-type inhibitors have been reported previously; however, the mechanism of action is not well established. in this study, the activity of alocasin against aedes aegypti is demonstrated and the structure-activity relationship of this kunitz-type inhibitor is explained through x-ray structure analyses.201829737039
expression of a synthetic gene for the major cytotoxin (cyt1aa) of bacillus thuringiensis subsp. israelensis in the chloroplast of wild-type chlamydomonas.chlamydomonas reinhardtii (chlamydomonas) strains that are toxic to mosquito larvae because they express chloroplast transgenes that are based on the mosquitocidal proteins of bacillus thuringiensis subsp. israelensis (bti) could be very useful in mosquito control. chlamydomonas has several advantages for this approach, including genetic controls not generally available with industrial algae. the bti toxin is produced by sporulating bacteria and has been used for mosquito control for >30 years w ...201829738473
complete genome of aedes aegypti anphevirus in the aag2 mosquito cell line.a novel negative-sense rna virus, aedes aegypti anphevirus, was recently identified in wild aedes aegypti mosquitoes. we show that this virus is also present in the aag2 aedes aegypti cell line and characterize its complete genome and evolutionary history. the aedes aegypti anphevirus genome is estimated to be 12 916 nucleotides in length, contains four genes and has a genome structure similar to that of other anpheviruses. phylogenetically, aedes aegypti anphevirus falls within an unclassified ...201829741476
atypical neurological manifestations of chikungunya fever: two case reports.chikungunya fever (chik) is an arboviral disease which is transmitted by a mosquito bite (aedes aegypti). fever, polyarthralgia, and maculopapular rash are hallmark clinical manifestations of this disease. these manifestations are self-liming and complete recovery is seen in most of the cases. however, atypical and lethal complications such as myocarditis, hepatitis, guillain-barre syndrome (gbs), and meningoencephalitis have been reported in few cases of chik. we hereby report two cases of chik ...201829743772
seasonal temperature variation influences climate suitability for dengue, chikungunya, and zika transmission.dengue, chikungunya, and zika virus epidemics transmitted by aedes aegypti mosquitoes have recently (re)emerged and spread throughout the americas, southeast asia, the pacific islands, and elsewhere. understanding how environmental conditions affect epidemic dynamics is critical for predicting and responding to the geographic and seasonal spread of disease. specifically, we lack a mechanistic understanding of how seasonal variation in temperature affects epidemic magnitude and duration. here, we ...201829746468
a new "american" subgroup of african-lineage chikungunya virus detected in and isolated from mosquitoes collected in haiti, 2016.as part of on-going arboviral surveillance activity in a semi-rural region in haiti, chikungunya virus (chikv)-positive mosquito pools were identified in 2014 (the peak of the caribbean asian-clade epidemic), and again in 2016 by rt-pcr. in 2014, chikv was only identified in aedes aegypti (11 positive pools/124 screened). in contrast, in sampling in 2016, chikv was not identified in ae. aegypti, but, rather, in (a) a female aedes albopictus pool, and (b) a female culex quinquefasciatus pool. gen ...201829746539
experimental zika virus infection in aedes aegypti: susceptibility, transmission & co-infection with dengue & chikungunya viruses.there are reports about the susceptibility of aedes mosquitoes to zikv from various countries, however, no such information is available from indian sub-continent, although, high level of group cross-reactivity of zikv with other flaviviruses has been reported. during outbreak situations, many cases of dengue (den) and chikungunya (chik) are reported. in such scenario, vector mosquitoes are likely to get co-infection/secondary-infection with one or other virus. the present study was carried out ...201829749366
effect of uv-b irradiation on water-suspended metarhizium anisopliae s.l. (hypocreales: clavicipitaceae) conidia and their larvicidal activity in aedes aegypti (diptera: culicidae).ultraviolet (uv) radiation is a key limiting factor for biological pest control with entomopathogenic fungi. while little is known about the impact of uv on metarhizium anisopliae metchnikoff (sorokin) (hypocreales: clavicipitaceae) conidia in aquatic mosquito-breeding sites, this study determined the effect of uv-b on the viability and virulence of m. anisopliae sensu lato (s.l.) strain ip 46 in the laboratory against aedes aegypti (l.) (diptera: culicidae) larvae. conidia were treated in cups ...201829750411
molecular responses to the zika virus in mosquitoes.the zika virus (zikv), originally discovered in 1947, did not become a major concern until the virus swept across the pacific and into the americas in the last decade, bringing with it news of neurological complications and birth defects in zikv affected areas. this prompted researchers to dissect the molecular interactions between zikv and the mosquito vector in an attempt to better understand not only the changes that occur upon infection, but to also identify molecules that may potentially en ...201829751526
a wolbachia triple-strain infection generates self-incompatibility in aedes albopictus and transmission instability in aedes aegypti.artificially-introduced transinfections of the intracellular bacterium wolbachia pipientis have the potential to reduce the vectorial capacity of mosquito populations for viruses such as dengue and chikungunya. aedes albopictus has two native strains of wolbachia, but their replacement with the non-native wmel strain blocks transmission of both viruses. the pattern of cytoplasmic incompatiiblity generated by wmel with wild-types is bidirectional. novel-plus-native-strain co-infection is predicte ...201829751814
dynamics of dengue disease with human and vector mobility.dengue is a vector borne disease transmitted to humans by aedes aegypti mosquitoes carrying virus of different serotypes. dengue exhibits complex spatial and temporal dynamics, influenced by various biological, human and environmental factors. in this work, we study the dengue spread for a single serotype (denv-1) including statistical models of human mobility with exponential step length distribution, by using reaction-diffusion equations and stochastic cellular automata (sca) approach. we anal ...201829751893
sargassum wightii-synthesized zno nanoparticles - from antibacterial and insecticidal activity to immunostimulatory effects on the green tiger shrimp penaeus semisulcatus.the green synthesis of metal nanoparticles using phytochemical from marine seaweeds is a fast-growing research field in nanotechnology. here, the biosynthesis of zinc oxide nanoparticles was achieved using the hot water extract of sargassum wightii. the hot water extract prepared from s. wightii (h sw) and zno nps were studied by uv-visible and ftir spectroscopy, sem and xrd. then, both products were evaluated for antibiofilm activity towards aquatic pathogens. the nanoparticles' immunostimulati ...201829754050
de novo dna synthesis in aedes aegypti midgut cells as a complementary strategy to limit dengue viral replication.aedes aegypti is the main vector of dengue virus, carrying the virus during the whole mosquito life post-infection. few mosquito fitness costs have been associated to the virus infection, thereby allowing for a swift dissemination. in order to diminish the mosquito population, public health agency use persistent chemicals with environmental impact for disease control. most countries barely use biological controls, if at all. with the purpose of developing novel dengue control strategies, a detai ...201829755433
effects of scarcity and excess of larval food on life history traits of aedes aegypti (diptera: culicidae).few studies have assessed the effects of food scarcity or excess on the life history traits of aedes aegypti (l.) (diptera: culicidae) independently from larval density. we assessed immature survival, development time, and adult size in relation to food availability. we reared cohorts of 30 ae. aegypti larvae from newly hatched to adult emergence with different food availability. food conditions were kept constant by transferring larvae each day to a new food solution. immature development was c ...201829757503
controversies over the scientific name of the principal mosquito vector of yellow fever virus - expediency versus validity.the history of the scientific name of the yellow fever mosquito, the vector of yellow fever virus, ranges from 1757 to the early twenty-first century. in his 1757 work iter palaestinum, frederic hasselquist gave the name culex aegypti to a mosquito species responsible for fierce attacks on humans in egypt. that name was never later ascribed to hasselquist as author, but to linnaeus, although the name never appeared in any of linnaeus' publications. in cuba, at the end of the nineteenth century, ...201829757506
mosquito traps for urban surveillance: collection efficacy and potential for use by citizen scientists.mosquito-borne diseases are a pervasive public health problem on a global scale, and effective management of them requires well-designed surveillance programs for both vectors and pathogens. mosquito traps are a common component of such programs, and their reach can be expanded by engaging citizen scientists. in this study in a southern australian city, we compared the mosquito collection efficacy of two types of traps and assessed their suitability for use in citizen science programs. bg sentin ...201829757507
diel patterns of aedes aegypti (diptera: culicidae) after resurgence in st. augustine, florida as collected by a mechanical rotator trap. 201829757509
two novel mutations in the voltage-gated sodium channel associated with knockdown resistance (kdr) in the dengue vector aedes aegypti in vietnam. 201829757514
a review of the ongoing research on zika virus treatment.the zika fever is an arboviral disease resulting from the infection with zika virus (zikv). the virus is transmitted to humans by the bite of aedes mosquitos, mainly aedes aegypti and aedes albopictus. zikv has been detected for decades in african and asian regions and, since 2007, has spread to other continents; among them, infections are most reported in the americas. this can be explained by the presence of vectors in highly populated and tropical regions where people are susceptible to conta ...201829758005
aedes aegypti(linnaeus) larvae from dengue outbreak areas in selangor showing resistance to pyrethroids but susceptible to organophosphates.the resistance status of selangor aedes aegypti (linnaeus) larvae against four major groups of insecticides (i.e., organochlorines, carbamates, organophosphates and pyrethroids) was investigated. aedes aegypti were susceptible against temephos (organophosphate), although resistance (rr50 = 0.21-2.64) may be developing. the insecticides susceptibility status of ae. aegypti larvae were found heterogeneous among the different study sites. results showed that ae. aegypti larvae from klang, sabak ber ...201829758171
threats of zika virus transmission for asia and its hindu-kush himalayan region.asia and its hindu kush himalayan (hkh) region is particularly vulnerable to environmental change, especially climate and land use changes further influenced by rapid population growth, high level of poverty and unsustainable development. asia has been a hotspot of dengue fever and chikungunya mainly due to its dense human population, unplanned urbanization and poverty. in an urban cycle, dengue virus (denv) and chikungunya virus (chikv) are transmitted by aedes aegypti and ae. albopictus mosqui ...201829759076
effects of light and temperature on daily activity and clock gene expression in two mosquito disease vectors.most organisms feature an endogenous circadian clock capable of synchronization with their environment. the most well-known synchronizing agents are light and temperature. the circadian clock of mosquitoes, vectors of many pathogens, drives important behaviors related to vectoral capacity, including oviposition, host seeking, and hematophagy. main clock gene expression, as well as locomotor activity patterns, has been identified in aedes aegypti and culex quinquefasciatus under artificial light- ...201829761749
pyrethroid insecticides maintain repellent effect on knock-down resistant populations of aedes aegypti mosquitoes.pyrethroid-treated clothing is commonly worn for protection against mosquitoes; pyrethroids are both insecticides and repellents. pyrethroid resistance has become increasingly common in aedes aegypti, the vector of dengue, zika, and other arboviruses, but it is not clear whether resistance is associated with reductions in repellency. in order to determine whether long-lasting permethrin impregnated (llpi) clothing is protective, we used aedes aegypti from new orleans, la (pyrethroid-sensitive) a ...201829763445
ns1 codon usage adaptation to humans in pandemic zika virus.zika virus (zikv) was recognised as a zoonotic pathogen in africa and southeastern asia. human infections were infrequently reported until 2007, when the first known epidemic occurred in micronesia. after 2013, the asian lineage of zikv spread along the pacific islands and americas, causing severe outbreaks with millions of human infections. the recent human infections of zikv were also associated with severe complications, such as an increase in cases of guillain-barre syndrome and the emergenc ...201829768530
efficacy of the main repellents available in the brazilian market against aedes aegypti bites under concentrations applied to pediatric populations. 201829768569
ornamental bromeliads of miami-dade county, florida are important breeding sites for aedes aegypti (diptera: culicidae).a major public health concern is the emergence and geographical spread of vector-borne diseases such as zika and yellow fever. ornamental bromeliads retaining water in their leaf axils represent potential breeding sites for mosquitoes. as the role of ornamental bromeliads in breeding aedes aegypti in miami-dade county, florida is unknown, we hypothesize that ornamental bromeliads are important breeding sites for ae. aegypti. our objective was to survey bromeliads in areas with high densities of ...201829769105
niche conservatism of aedes albopictus and aedes aegypti - two mosquito species with different invasion histories.biological invasions have been associated with niche changes; however, their occurrence is still debated. we assess whether climatic niches between native and non-native ranges have changed during the invasion process using two globally spread mosquitoes as model species, aedes albopictus and aedes aegypti. considering the different time spans since their invasions (>300 vs. 30-40 years), niche changes were expected to be more likely for ae. aegypti than for ae. albopictus. we used temperature a ...201829769652
is there a risk of chikungunya transmission in canada?an outbreak of chikungunya virus infection is currently underway in the caribbean, central america and south america, and autochthonous (local or indigenous) transmission has occurred in the southeastern united states. the mosquito species known to transmit chikungunya-aedes aegypti and aedes albopictus-are not known to reside in canada at this time. but how comfortable can we be that this situation will continue? here we explore four key conditions that must be met for transmission of chikungun ...201529769915
tracking aedes aegypti (diptera: culicidae) larval behavior across development: effects of temperature and nutrients on individuals' foraging behavior.immature mosquitoes alter their foraging behavior in response to variation in nutrients, predators, and temperature, with consequences on the adult stage where pathogens are transmitted. these patterns of behavior have not been described with respect to both developmental stage and environmental variation, nor has behavior been examined within an individual across instars. we hypothesized that individual larvae have distinct behavioral syndromes, and predict that the rank of foraging activity in ...201829771372
encapsidated host factors in alphavirus particles influence midgut infection of aedes aegypti.transmission of mosquito-borne viruses requires the efficient infection of both a permissive vertebrate host and a competent mosquito vector. the infectivity of sindbis virus (sinv), the type species of the alphavirus genus, is influenced by both the original and new host cell. we have shown that infection of vertebrate cells by sinv, chikungunya virus (chikv), and ross river virus (rrv) produces two subpopulations of virus particles separable based on density. in contrast, a single population o ...201829772674
urban environmental clustering to assess the spatial dynamics of aedes aegypti breeding sites.the identification of aedes aegypti breeding hotspots in urban areas is crucial for the rational design of control strategies against this disease vector. remote sensing and geographic information systems offer valuable tools for mapping habitat suitability of a given area. however, predicting species occurrences by means of probability distribution maps based on transversal entomological surveys has limited utility for local authorities. the aim of the present study was to carefully examine the ...201829772886
the influence of urban heat islands and socioeconomic factors on the spatial distribution of aedes aegypti larval habitats.we addressed the potential associations among the temporal and spatial distribution of larval habitats of aedes (stegomyia) aegypti, the presence of urban heat islands and socioeconomic factors. data on larval habitats were collected in santa bárbara d'oeste, são paulo, brazil, from 2004 to 2006, and spatial and temporal variations were analysed using a wavelet-based approach. we quantified urban heat islands by calculating surface temperatures using the results of wavelet analyses and grey leve ...201829772891
biological control of anopheles darlingi, aedes aegypti and culex quinquefasciatus larvae using shrimps.mosquitoes can act as vectors of important diseases such as malaria, dengue, zika virus, yellow fever, chikungunya and mayaro fever, in addition to filariasis. the use of insecticides, larvicides, bed nets and repellents, besides the use of drugs as chemoprevention and the treatment of the sick are currently the pillars of the control of these vectors. we studied the biological control against of anopheles darlingi, aedes aegypti and culex quinquefasciatus larvae using shrimps of the species m. ...201729774286
evaluation of phytosynthesised silver nanoparticles from leaf extracts of leucas aspera and hyptis suaveolens and their larvicidal activity against malaria, dengue and filariasis vectors.the present study deals with the green synthesis of silver nanoparticle from the aqueous leaf extracts of leucas aspera and hyptis suaveolens as reducing agent and to investigate the larvicidal activity of synthesized silver nanoparticles. the synthesized silver nanoparticles were characterized by ultraviolet and visible absorption spectroscopy (uv), fourier transform-infrared spectroscopy (ft-ir), x-ray spectroscopy (xrd), field emission scanning electron microscope (fesem) and high-resonance t ...201729774292
use of bead-based serologic assay to evaluate chikungunya virus epidemic, haiti.the index case of chikungunya virus (chikv) in haiti was reported during early 2014; the vector, the pervasive aedes aegypti mosquito, promoted rapid spread throughout the country. during december 2014-february 2015, we collected blood samples from 4,438 persons at 154 sites (62 urban, 92 rural) throughout haiti and measured chikv igg by using a multiplex bead assay. overall chikv seroprevalence was 57.9%; differences between rural (mean 44.9%) and urban (mean 78.4%) areas were pronounced. logis ...201829774861
modeling dengue vector population using remotely sensed data and machine learning.mosquitoes are vectors of many human diseases. in particular, aedes ægypti (linnaeus) is the main vector for chikungunya, dengue, and zika viruses in latin america and it represents a global threat. public health policies that aim at combating this vector require dependable and timely information, which is usually expensive to obtain with field campaigns. for this reason, several efforts have been done to use remote sensing due to its reduced cost. the present work includes the temporal modeling ...201829777650
chemical profile and pharmacological effects of the resin and essential oil from bursera slechtendalii: a medicinal "copal tree" of southern mexico.this study reports on the volatile profile and pharmacological activities of the essential oil and resin from bursera slechtendalii, an aromatic medicinal tree commercialized in southern mexico. the essential oil contained 25 volatiles including p-cymen-8-ol (25.4-35.7%), n-hexyl hexanoate (6.8-17.6%) and n-octyl hexanoate (7.6-14.8%), whereas the crystallized resin contained 14 volatiles, including n-octyl acetate (29.6-34.2%), limonene (15.8-18.7%) and n-octyl butyrate (12.6-17-6%). the inhala ...201829777752
genome sequence analysis of a novel bacillus thuringiensis strain blb406 active against aedes aegypti larvae, a novel potential bioinsecticide.blb406 is a novel isolate of bacillus thuringiensis with a larvicidal activity against aedes aegypti larvae. it displays original plasmidic and crystal protein patterns. the present work reported molecular and bioinformatic analyses for the genome sequence of blb406 using miseq illumina next-generation sequencing technology. the reads were assembled by velvet tool. using rast program and pgaap, the genome of blb406 strain was shown to contain 6297 genes corresponding to 5924 protein coding seque ...201829778876
zika virus: report from the task force on tropical diseases by the world federation of societies of intensive and critical care medicine.zika fever is an acute infectious disease caused by the zika virus (zikv), an rna virus belonging to the genus flavivirus. zikv can be transmitted by bites from aedes aegypti and aedes albopictus species, sexual activity, blood transfusion and from mother to fetus during pregnancy and delivery. a total of 50 territories and countries in the americas had reported zikv infections. for those with symptoms, the presentation is similar to dengue fever with body aches, joint pain, fatigue, malaise, fe ...201829779827
regulation of midgut cell proliferation impacts aedes aegypti susceptibility to dengue virus.aedes aegypti is the vector of some of the most important vector-borne diseases like dengue, chikungunya, zika and yellow fever, affecting millions of people worldwide. the cellular processes that follow a blood meal in the mosquito midgut are directly associated with pathogen transmission. we studied the homeostatic response of the midgut against oxidative stress, as well as bacterial and dengue virus (denv) infections, focusing on the proliferative ability of the intestinal stem cells (isc). i ...201829782512
efficacy of lysinibacillus sphaericus against mixed-cultures of field-collected and laboratory larvae of aedes aegypti and culex quinquefasciatus.lysinibacillus sphaericus (bacillales: planococcaceae) is a spore-forming bacillus used for the biological control of mosquitoes (diptera: culicidae) due to its larvicidal activity determined by various toxins and s-layer protein produced either during sporulation or by the vegetative cell. aedes aegypti and culex quinquefasciatus are the vectors of arboviruses that cause tropical diseases representing a current public health problem. both species may coexist in the same larval development sites ...201929784071
a multi-faceted pandemic: a review of the state of knowledge on the zika virus.while until recently the small and isolated zika outbreaks in eastern asia and pacific islands had been overlooked, the large-scale outbreak that started in brazil in 2015 and the increase of microcephaly cases in the same place and time made media headlines. considered as harmless until recently, zika has given rise to an important global crisis that poses not only health challenges but also environmental, economical, social, and ethical challenges for states and people around the world. the ma ...201829785319
threshold dynamics of a temperature-dependent stage-structured mosquito population model with nested delays.mosquito-borne diseases remain a significant threat to public health and economics. since mosquitoes are quite sensitive to temperature, global warming may not only worsen the disease transmission case in current endemic areas but also facilitate mosquito population together with pathogens to establish in new regions. therefore, understanding mosquito population dynamics under the impact of temperature is considerably important for making disease control policies. in this paper, we develop a sta ...201829785519
surveillance of mosquitoes (diptera: culicidae) in southern iowa, 2016.the mosquito fauna of iowa has been extensively investigated over several decades, providing a wealth of information regarding species distributions, relative abundance, temporal activity patterns, and identifying vectors of medical importance. however, these investigations have had unequal coverage, leaving the mosquito fauna in some parts of the state, including southern iowa, largely uncharacterized. with the heightened public health threat of zika virus in the summer of 2016, greater emphasi ...201829788488
codon adaptation biases among sylvatic and urban genotypes of dengue virus type 2.dengue virus (denv) emerged from the sylvatic environment and colonized urban settings, being sustained in a human-aedes-human transmission chain, mainly by the bites of females of the anthropophilic species aedes aegypti. herein, we sought evidence for fine-tuning in viral codon usage, possibly due to viral adaptation to human transmission. we compared the codon adaptation of denv serotype 2 (denv-2) genotypes from urban and sylvatic habitats and tried to correlate the findings with key evoluti ...201829792991
vapor toxicity of five volatile pyrethroids against aedes aegypti, aedes albopictus, culex quinquefasciatus, and anopheles quadrimaculatus (diptera: culicidae).mosquito mortality has been documented in numerous studies of spatial repellents but the concentration-dependent toxicity of spatial repellent vapors has not been documented. to address this issue, prallethrin, flumethrin, metofluthrin, transfluthrin, and meperfluthrin were selected for comparative study against aedes albopictus (skuse), ae. aegypti (l.), culex quinquefasciatus say, and anopheles quadrimaculatus say. mosquito were exposed to vapors of each chemical for 2, 4, and 24 h with mortal ...201829797795
impact of short-term temperature challenges on the larvicidal activities of the entomopathogenic watermold leptolegnia chapmanii against aedes aegypti, and development on infected dead larvae.the oomycete leptolegnia chapmanii is among the most promising entomopathogens for biological control of aedes aegypti. this mosquito vector breeds in small water collections, where this aquatic watermold pathogen can face short-term scenarios of challenging high or low temperatures during changing ambient conditions, but it is yet not well understood how extreme temperatures might affect the virulence and recycling capacities of this pathogen. we tested the effect of short-term exposure of ency ...201829801786
rapid, noninvasive detection of zika virus in aedes aegypti mosquitoes by near-infrared spectroscopy.the accelerating global spread of arboviruses, such as zika virus (zikv), highlights the need for more proactive mosquito surveillance. however, a major challenge during arbovirus outbreaks has been the lack of rapid and affordable tests for pathogen detection in mosquitoes. we show for the first time that near-infrared spectroscopy (nirs) is a rapid, reagent-free, and cost-effective tool that can be used to noninvasively detect zikv in heads and thoraces of intact aedes aegypti mosquitoes with ...201829806030
evaluation of larvicidal, adulticidal, and anticholinesterase activities of essential oils of illicium verum hook. f., pimenta dioica (l.) merr., and myristica fragrans houtt. against zika virus vectors.aedes aegypti is the vector responsible for transmitting pathogens that cause various infectious diseases, such as dengue, zika, yellow fever, and chikungunya, worrying health authorities in the tropics. due to resistance of mosquitoes to synthetic insecticides, the search for more effective insecticidal agents becomes crucial. the aim of this study was to verify the larvicidal, adulticidal, and anticholinesterase activities of the essential oils of the illicium verum (eoiv), pimenta dioica (eop ...201829808407
the risk of urban yellow fever resurgence in aedes-infested american cities.aedes aegypti, historically known as yellow fever (yf) mosquito, transmits a great number of other viruses such as dengue, west nile, chikungunya, zika, mayaro and perhaps oropouche, among others. well established in africa and asia, aedes mosquitoes are now increasingly invading large parts of the american continent, and hence the risk of urban yf resurgence in the american cities should because of great concern to public health authorities. although no new urban cycle of yf was reported in the ...201829843824
back-to-the-future potential for autochthonous transmission of aedes aegypti-transmitted viruses in ethekwini and urban coastal kwazulu-natal province, south africa. 201829843848
structures of zika virus e & ns1: relations with virus infection and host immune responses.zika virus (zikv), first discovered in the zika forest in uganda in 1947 was understudied until the recent explosive epidemic in several south american countries where it has become strongly associated with congenital birth defects leading to severe cranial malformations and neurological conditions. the increase in number of case of microcephaly in newborn children associated with zikv infection triggered the world health organization to declare the epidemic as a public health emergency of inter ...201829845526
the use of wolbachia by the world mosquito program to interrupt transmission of aedes aegypti transmitted viruses.the biological control of mosquito transmission by the use of the naturally occurring insect-specific bacterial endosymbiont wolbachia has been successfully tested in small field trials. the approach has been translated successfully to larger field sites in townsville, australia and expanded to more than 10 countries through the eliminate dengue program. the broader application of the program beyond limiting the transmission of dengue and including other aedes aegypti borne mosquitoes has seen t ...201829845544
follow up of a robust meta-signature to identify zika virus infection in aedes aegypti: another brick in the wall.the mosquito aedes aegypti is the main vector of several arthropod-borne diseases that have global impacts. in a previous meta-analysis, our group identified a vector gene set containing 110 genes strongly associated with infections of dengue, west nile and yellow fever viruses. of these 110 genes, four genes allowed a highly accurate classification of infected status. more recently, a new study of ae. aegypti infected with zika virus (zikv) was published, providing new data to investigate wheth ...201829846381
[knowledge and practices in aedes aegypti control among different social subjects in salvador, bahia state, brazil].due to the persistence of dengue and other arbovirus infections in brazil, the government has stepped up measures to combat the aedes aegypti mosquito vector. the responsibilities of community endemic disease workers (cedw) and community health workers (chw) include acting as intermediaries and disseminating knowledge in the community. the aim of this study was to analyze knowledge and practices in dengue control by different social subjects: residents and cedw/chw. interviews were held with res ...201829846399
dengue in singapore from 2004 to 2016: cyclical epidemic patterns dominated by serotypes 1 and 2.singapore has experienced periodic dengue epidemics despite maintaining a low aedes house index. each epidemic was associated with a switch in the predominant serotype. we investigated the temporal dynamics of dengue fever and dengue virus (denv) and analyzed the epidemiological and entomological patterns of dengue in singapore from 2004 to 2016. the case surveillance is based on a mandatory notification system that requires all medical practitioners to report clinically suspected and laboratory ...201829848407
a highly secure method for rearing aedes aegypti mosquitoes.vector-borne infectious diseases are caused by pathogenic microorganisms transmitted mainly by blood-sucking arthropod vectors. in laboratories, the handling of insects carrying human pathogens requires extra caution because of safety concerns over their escape risk. based on standard insect containment practices, there have been cases where costly enhancements were required to definitely protect laboratory workers and neighbors from potential infection through mosquito bites. here, we developed ...201829849477
the awed trial (applying wolbachia to eliminate dengue) to assess the efficacy of wolbachia-infected mosquito deployments to reduce dengue incidence in yogyakarta, indonesia: study protocol for a cluster randomised controlled trial.dengue and other arboviruses transmitted by aedes aegypti mosquitoes, including zika and chikungunya, present an increasing public health challenge in tropical regions. current vector control strategies have failed to curb disease transmission, but continue to be employed despite the absence of robust evidence for their effectiveness or optimal implementation. the world mosquito program has developed a novel approach to arbovirus control using ae. aegypti stably transfected with wolbachia bacter ...201829855331
exposure of a diurnal mosquito vector to floral mimics: foraging responses, feeding patterns, and significance for sugar bait technology.food location by mosquitoes is mediated by resource-derived olfactory and visual signals. smell sensation is intermittent and dependent on the environment, whereas visual signals are continual and precede olfactory cues. success of mosquito bait technology, where olfactory cues are used for attraction, is being impeded by reduced attractiveness. despite proof that mosquitoes respond to colored objects, including those mimicking floral shape, and that they can discriminate among flowers, the impa ...201829856985
technologies to combat aedes mosquitoes: a model based on smart city.aedes aegypti and aedes albopictus mosquitoes are responsible for the transmission of diseases such as dengue fever, yellow fever, chikungunya fever, zika virus fever, some of which can cause irreversible central nervous system problems and death. this study investigates what technologies are being used for combatting and monitoring the aedes mosquitoes and to propose joining these technologies into a single and complete solution using the smart cities concept. a search for newscasts on google a ...201829857404
molecular and functional characterization of single-box high-mobility group b (hmgb) chromosomal protein from aedes aegypti.high-mobility group b (hmgb) proteins have highly conserved, unique dna-binding domains, hmg boxes, that can bind non-b-type dna structures, such as bent, kinked and unwound structures, with high affinity. hmgb proteins also promote dna bending, looping and unwinding. in this study, we determined the role of the aedes aegypti single hmg-box domain protein aahmgb; characterized its structure, spatiotemporal expression levels, subcellular localization, and nucleic acid binding activities; and comp ...201829859286
pyrethroid resistance in the dengue vector aedes aegypti in southeast asia: present situation and prospects for management.human arboviral diseases transmitted by aedes aegypti such as dengue, zika, chikungunya and yellow fever remain global public health threats to date. of these diseases, dengue fever is particularly prevalent in southeast asia. relentless vector control efforts are performed to curtail disease transmissions through which pyrethroid insecticides are broadly used as the first line of defense to control ae. aegypti, especially in the course of disease outbreaks. here, we compile the largest contempo ...201829866193
effect of the topical repellent para-menthane-3,8-diol on blood feeding behavior and fecundity of the dengue virus vector aedes aegypti.dengue fever is an acute disease caused by the dengue virus and transmitted primarily by the mosquito aedes aegypti. the current strategy for dengue prevention is vector control including the use of topical repellents to reduce mosquito biting. although n,n-diethyl-m-methylbenzamide (deet) is the most common active ingredient in topical repellent products, para-menthane-3,8-diol (pmd) is also used commercially. studies have indicated pmd reduced biting by 90⁻95% for up to 6⁻8 h, similar to the e ...201829867036
serosurvey of human antibodies recognizing aedes aegypti d7 salivary proteins in colombia.dengue is one of the most geographically significant mosquito-borne viral diseases transmitted by aedes mosquitoes. during blood feeding, mosquitoes deposit salivary proteins that induce antibody responses. these can be related to the intensity of exposure to bites. some mosquito salivary proteins, such as d7 proteins, are known as potent allergens. the antibody response to d7 proteins can be used as a marker to evaluate the risk of exposure and disease transmission and provide critical informat ...201829868532
differential effects of temperature and mosquito genetics determine transmissibility of arboviruses by aedes aegypti in argentina.aedes aegypti (l.) (diptera: culicidae) have a global distribution and are the primary vector of a number of mosquito-borne viruses responsible for epidemics throughout the americas. as in much of south america, the threat from pathogens including dengue virus (denv; flaviviridae, flavivirus) and chikungunya virus (chikv; togaviridae, alphavirus) has increased in argentina in recent years. the complexity of transmission cycles makes predicting the occurrence and intensity of arbovirus outbreaks ...201829869610
vertical transmission of dengue virus in the yogyakarta airport area.international health regulations controls international travel including human movement, disease vector, and imported items to prevent the spread of dengue, especially in seaports, airports, and border crossing posts. this study aimed to determine dengue transovarial transmission index (tti) and distribution of dengue virus in the areas around adisucipto airport of yogyakarta, indonesia.201829871615
effects of temephos, permethrin, and eucalyptus nitens essential oil on survival and swimming behavior of aedes aegypti and anopheles pseudopunctipennis (diptera: culicidae) larvae.an essential strategy to deal with mosquito-borne diseases is the control of larvae in their development sites. the mosquitoes anopheles pseudopunctipennis (theobald) (diptera: culicidae), a malaria vector, and aedes aegypti (l.) (diptera: culicidae), vector of dengue, zika, yellow fever, and chikungunya viruses, breed in very different habitats. insecticide treatments of mosquito larvae focus mainly on their lethal effects. however, insecticide degradation or the poor dosage of larvicides will ...201829873741
multiscale approach to deciphering the molecular mechanisms involved in the direct and intergenerational effect of ibuprofen on mosquito aedes aegypti.the anti-inflammatory ibuprofen is a ubiquitous surface water contaminant. however, the chronic impact of this pharmaceutical on aquatic invertebrate populations remains poorly understood. in model insect aedes aegypti, we investigated the intergenerational consequences of parental chronic exposure to an environmentally relevant concentration of ibuprofen. while exposed individuals did not show any phenotypic changes, their progeny showed accelerated development and an increased tolerance to sta ...201829874051
viral determinants and vector competence of zika virus transmission.zika virus (zikv) has emerged as a new global health threat. since its first discovery in zika forest in uganda, this virus has been isolated from several mosquito species, including aedes aegypti and aedes albopictus. the geographical distribution of these mosquito species across tropical and subtropical regions has led to several outbreaks, including the recent pandemic in brazil, followed by the pacific islands and other areas of north and south america. this has gained attention of the scien ...201829875751
target and non-target toxicity of fern extracts against mosquito vectors and beneficial aquatic organisms.dengue and malaria are significant mosquito-borne diseases that are rapidly spread worldwide, mainly in temperate countries. pteridophytes were identified to be a significant source of novel mosquitocidal agents. the present research was to explore the eco-friendly larvicides from methanol extracts of ferns, viz., actiniopteris radiata, adiantum caudatum, cheilanthes swartzii, hemionitis arifolia and lycopodium clavatum. the larvicidal potential of the extracts screened using larvae of dengue ve ...201829885618
a transcriptomic survey of the impact of environmental stress on response to dengue virus in the mosquito, aedes aegypti.populations of aedes aegypti naturally exhibit variable susceptibility to dengue viruses. this natural variation can be impacted by nutritional stress resulting from larval-stage crowding, indicating the influence of environment components on the adult mosquito immune response. in particular, larval crowding was previously shown to reduce the susceptibility of adult females of a trinidad field isolate of a. aegypti to the dengue serotype 2 (jam1409) virus. here, we present the first whole transc ...201829889847
what does the future hold for yellow fever virus? (i).the recent resurgence of yellow fever virus (yfv) activity in the tropical regions of africa and south america has sparked renewed interest in this infamous arboviral disease. yellow fever virus had been a human plague for centuries prior to the identification of its urban transmission vector, the aedes (stegomyia) aegypti (linnaeus) mosquito species, and the development of an efficient live-attenuated vaccine, the yf-17d strain. the combination of vector-control measures and vaccination campaig ...201829890711
evaluation of the effects of the larvicides temephos on reproductive performance, embryofetal development and dna integrity of swiss mice.temephos is considered the gold standard by the ministry of health for controlling the larvae of the mosquito aedes aegypti. the present study evaluated the effects of temephos larvicide on the reproductive performance, embryo-fetal development and dna integrity of swiss mice. this study used 30 pregnant female mice: 10 were controls treated with drinking water at a dosage of 0.1 ml/10 g (body weight - b.w., administered orally - a.o.), and 20 were treated with temephos at doses of 0.0043 mg/kg ...201829891373
signifying zika: heterogeneity in the representations of the virus by history of infection.despite having been broadly advertised by the mass media, many negative consequences of the zika virus have been less significant than originally predicted. it is likely that after a few months from the epidemic's onset, personal experience with the virus has altered the person's way to deal with the disease. this study explores the relation between exposure to zika virus and the social representation of the epidemic. more specifically, one analyzes if increased exposure to the risk of zika infe ...201829898004
restoration of pyrethroid susceptibility in a highly resistant aedes aegypti population.insecticide resistance has evolved in disease vectors worldwide, creating the urgent need to either develop new control methods or restore insecticide susceptibility to regain use of existing tools. here we show that phenotypic susceptibility can be restored in a highly resistant field-derived strain of aedes aegypti in only 10 generations through rearing them in the absence of insecticide.201829899128
lack of evidence for natural wolbachia infections in aedes aegypti (diptera: culicidae).wolbachia is a genus of endosymbiotic bacteria that infects 66% of all insect species. its major impact on insects is in reproduction: sterility, production of one sex, and/or parthenogenesis. another effect was discovered when the disease-transmitting mosquito, aedes aegypti linnaeus (diptera: culicidae), was infected with wolbachia isolated from drosophila: infected female mosquitoes became less capable of transmitting diseases such as dengue fever and chikungunya. this has led to releases of ...201829901734
prediction and prevention of urban arbovirus epidemics: a challenge for the global virology community.the recent emergence and rapid spread of zika virus in tropical regions of the western hemisphere took arbovirologists and public health officials by surprise, and the earlier transfers of west nile and chikungunya viruses from the old to the new world were also unexpected. these pandemics underscore the increasing threat of zoonotic arboviruses, especially those that are capable of entering into human-amplified, urban transmission cycles transmitted by aedes (stegomyia) aegypti and sometimes ot ...201829906475
screening of natural wolbachia infection in aedes aegypti, aedes taeniorhynchus and culex quinquefasciatus from guadeloupe (french west indies).guadeloupe islands are threatened by several mosquito-borne viruses such as dengue, chikungunya, zika and west nile virus. it appears essential to look for alternative mosquito control methods such as the incompatible insect technique (itt) aiming at sterilizing wild females by inundative releases of incompatible males. before considering the implementation of such a strategy, the characterization of genetic diversity of the endocellular bacterium wolbachia regarding the local mosquito populatio ...201829908171
salinity tolerant aedes aegypti and ae. albopictus-infection with dengue virus and contribution to dengue transmission in a coastal peninsula.aedes aegypti and ae. albopictus are major arboviral vectors that are considered to lay eggs, and undergo preimaginal development only in fresh water collections. however, recently they have been also shown to develop in coastal brackish water habitats. the ability of the biologically variant salinity-tolerant aedes vectors to transmit arboviral diseases is unknown. we therefore, investigated the infection of salinity-tolerant aedes mosquitoes with dengue virus (denv) and analysed dengue inciden ...201829916445
human blood as the only source of aedes aegypti in churches from merida, yucatan, mexico. 201929916450
field testing & evaluation of the efficacy & duration of effectiveness of a biolarvicide, bactivec® sc (bacillus thuringiensis var. israelensis sh-14) in bengaluru, india.different formulations of bacillus thuringiensis var. israelensis (bti) have been tested against different mosquito vectors and other insects for their residual activity. in the present study, the efficacy and residual activity of a new formulation of bti (bactivec suspension concentrate) were evaluated against immature stages of anopheles stephensi liston (diptera: culicidae), aedes aegypti linnaeus (diptera: culicidae) and culex quinquefasciatus say (diptera: culicidae), in natural habitats in ...201829923520
hydrophobic nanoprecipitates of β-cyclodextrin/avermectins inclusion compounds reveal insecticide activity against aedes aegypti larvae and low toxicity against fibroblasts.in the present work, hydrophobic nanoprecipitates (hnps) of inclusion complexes formed between β-cyclodextrin (βcd) and the avermectins (avms) named eprinomectin (epri) and ivermectin (iver) were synthesized and characterized, and their larvicidal activity against aedes aegypti and human safety against fibroblasts were evaluated. initially, thermogravimetric analysis/differential thermal analysis data revealed that inclusion increased the thermal stability of avms in the presence of βcd. nuclear ...201829925239
vector competence of aedes aegypti, culex tarsalis, and culex quinquefasciatus from california for zika virus.zika virus (zikv) has emerged since 2013 as a significant global human health threat following outbreaks in the pacific islands and rapid spread throughout south and central america. severe congenital and neurological sequelae have been linked to zikv infections. assessing the ability of common mosquito species to transmit zikv and characterizing variation in mosquito transmission of different zikv strains is important for estimating regional outbreak potential and for prioritizing local mosquit ...201829927940
risk factors for the presence of dengue vector mosquitoes, and determinants of their prevalence and larval site selection in dhaka, bangladesh.dengue viruses are responsible for over 100 million infections a year worldwide and are a public health concern in bangladesh. although risk of transmission is high, data on vector population characteristics are scanty in bangladesh; therefore, a comprehensive prediction of the patterns of local virus transmission is not possible. recognizing these gaps, multi-year entomological surveys were carried out in dhaka, where the disease is most frequently reported. the specific objectives of the prese ...201829928055
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