Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| diversity, evolution and medical applications of insect antimicrobial peptides. | antimicrobial peptides (amps) are short proteins with antimicrobial activity. a large portion of known amps originate from insects, and the number and diversity of these molecules in different species varies considerably. insect amps represent a potential source of alternative antibiotics to address the limitation of current antibiotics, which has been caused by the emergence and spread of multidrug-resistant pathogens. to get more insight into amps, we investigated the diversity and evolution o ... | 2016 | 27160593 |
| histopathology of aedes aegypti (diptera: culicidae) larvae parasitized by reesimermis nielseni (nematoda: mermithidae). | 2016 | 4761359 | |
| esterase polymorphism in aedes aegypti: the genetics and k m values of electrophoretically heterogeneous forms. | 2016 | 5038250 | |
| frequency of blood feeding in the mosquito aedes aegypti. | 2016 | 4937213 | |
| systemically and cutaneously distributed ectoparasiticides: a review of the efficacy against ticks and fleas on dogs. | acaricidal (tick) and insecticidal (flea) efficacy of systemically and cutaneously distributed ectoparasiticide products for dogs are compared based on permethrin and fluralaner as representative molecules. results of efficacy studies against fleas and ticks are reviewed that show generally good to excellent results. both externally and systemically distributed treatments have benefits and weaknesses in potentially preventing pathogen transmission by these arthropod vectors.four general properti ... | 2016 | 27502490 |
| vaccines and immunization strategies for dengue prevention. | dengue is currently the most significant arboviral disease afflicting tropical and sub-tropical countries worldwide. dengue vaccines, such as the multivalent attenuated, chimeric, dna and inactivated vaccines, have been developed to prevent dengue infection in humans, and they function predominantly by stimulating immune responses against the dengue virus (denv) envelope (e) and nonstructural-1 proteins (ns1). of these vaccines, a live attenuated chimeric tetravalent denv vaccine developed by sa ... | 2016 | 27436365 |
| disease vectors in the era of next generation sequencing. | almost 20 % of all infectious human diseases are vector borne and, together, are responsible for over one million deaths per annum. over the past decade, the decreasing costs of massively parallel sequencing technologies have facilitated the agnostic interrogation of insect vector genomes, giving medical entomologists access to an ever-expanding volume of high-quality genomic and transcriptomic data. in this review, we highlight how genomics resources have provided new insights into the physiolo ... | 2016 | 27154554 |
| genomic insights into the ixodes scapularis tick vector of lyme disease. | ticks transmit more pathogens to humans and animals than any other arthropod. we describe the 2.1 gbp nuclear genome of the tick, ixodes scapularis (say), which vectors pathogens that cause lyme disease, human granulocytic anaplasmosis, babesiosis and other diseases. the large genome reflects accumulation of repetitive dna, new lineages of retro-transposons, and gene architecture patterns resembling ancient metazoans rather than pancrustaceans. annotation of scaffolds representing ∼57% of the ge ... | 2016 | 26856261 |
| identification of abcc2 as a binding protein of cry1ac on brush border membrane vesicles from helicoverpa armigera by an improved pull-down assay. | cry1ac toxin-binding proteins from helicoverpa armigera brush border membrane vesicles were identified by an improved pull-down method that involves coupling cry1ac to cnbr agarose combined with liquid chromatography-tandem mass spectrometry (lc-ms/ms). according to the lc-ms/ms results, cry1ac toxin could bind to six classes of aminopeptidase-n, alkaline phosphatase, cadherin-like protein, atp-binding cassette transporter subfamily c protein (abcc2), actin, atpase, polycalin, and some other pro ... | 2016 | 27037552 |
| unique features of a global human ectoparasite identified through sequencing of the bed bug genome. | the bed bug, cimex lectularius, has re-established itself as a ubiquitous human ectoparasite throughout much of the world during the past two decades. this global resurgence is likely linked to increased international travel and commerce in addition to widespread insecticide resistance. analyses of the c. lectularius sequenced genome (650 mb) and 14,220 predicted protein-coding genes provide a comprehensive representation of genes that are linked to traumatic insemination, a reduced chemosensory ... | 2016 | 26836814 |
| mosquito-transmitted viruses - the great brazilian challenge. | arboviruses pose a serious threat to public health worldwide, overloading the healthcare system and causing economic losses. these viruses form a very diverse group, and in brazil, arboviruses belonging to the families flaviviridae and togaviridae are predominant. unfortunately, the number of arboviruses increases in proportion with factors such as deforestation, poor sanitation, climate changes, and introduction of new viruses like chikungunya virus and zika virus. in brazil, dengue is endemic, ... | 2016 | 27818091 |
| the viral capping enzyme nsp1: a novel target for the inhibition of chikungunya virus infection. | the chikungunya virus (chikv) has become a substantial global health threat due to its massive re-emergence, the considerable disease burden and the lack of vaccines or therapeutics. we discovered a novel class of small molecules ([1,2,3]triazolo[4,5-d]pyrimidin-7(6h)-ones) with potent in vitro activity against chikv isolates from different geographical regions. drug-resistant variants were selected and these carried a p34s substitution in non-structural protein 1 (nsp1), the main enzyme involve ... | 2016 | 27545976 |
| mosquito communities and disease risk influenced by land use change and seasonality in the australian tropics. | anthropogenic land use changes have contributed considerably to the rise of emerging and re-emerging mosquito-borne diseases. these diseases appear to be increasing as a result of the novel juxtapositions of habitats and species that can result in new interchanges of vectors, diseases and hosts. we studied whether the mosquito community structure varied between habitats and seasons and whether known disease vectors displayed habitat preferences in tropical australia. | 2016 | 27388293 |
| alphavirus infection: host cell shut-off and inhibition of antiviral responses. | alphaviruses cause debilitating disease in humans and animals and are transmitted by blood-feeding arthropods, typically mosquitoes. with a traditional focus on two models, sindbis virus and semliki forest virus, alphavirus research has significantly intensified in the last decade partly due to the re-emergence and dramatic expansion of chikungunya virus in asia, europe, and the americas. as a consequence, alphavirus-host interactions are now understood in much more molecular detail, and importa ... | 2016 | 27294951 |
| design and testing of novel lethal ovitrap to reduce populations of aedes mosquitoes: community-based participatory research between industry, academia and communities in peru and thailand. | dengue virus (and chikungunya and zika viruses) is transmitted by aedes aegypti and aedes albopictus mosquitoes and causes considerable human morbidity and mortality. as there is currently no vaccine or chemoprophylaxis to protect people from dengue virus infection, vector control is the only viable option for disease prevention. the purpose of this paper is to illustrate the design and placement process for an attractive lethal ovitrap to reduce vector populations and to describe lessons learne ... | 2016 | 27532497 |
| wolbachia biocontrol strategies for arboviral diseases and the potential influence of resident wolbachia strains in mosquitoes. | arboviruses transmitted by mosquitoes are a major cause of human disease worldwide. the absence of vaccines and effective vector control strategies has resulted in the need for novel mosquito control strategies. the endosymbiotic bacterium wolbachia has been proposed to form the basis for an effective mosquito biocontrol strategy. resident strains of wolbachia inhibit viral replication in drosophila fruit flies and induce a reproductive phenotype known as cytoplasmic incompatibility that allows ... | 2016 | 26925368 |
| arbovirosis and potential transmission blocking vaccines. | infectious diseases caused by arboviruses (viruses transmitted by arthropods) are undergoing unprecedented epidemic activity and geographic expansion. with the recent introduction of west nile virus (1999), chikungunya virus (2013) and zika virus (2015) to the americas, stopping or even preventing the expansion of viruses into susceptible populations is an increasing concern. with a few exceptions, available vaccines protecting against arboviral infections are nonexistent and current disease pre ... | 2016 | 27664127 |
| characterization of drosophila cmp-sialic acid synthetase activity reveals unusual enzymatic properties. | cmp-sialic acid synthetase (csas) is a key enzyme of the sialylation pathway. csas produces the activated sugar donor, cmp-sialic acid, which serves as a substrate for sialyltransferases to modify glycan termini with sialic acid. unlike other animal csass that normally localize in the nucleus, drosophila melanogaster csas (dmcsas) localizes in the cell secretory compartment, predominantly in the golgi, which suggests that this enzyme has properties distinct from those of its vertebrate counterpa ... | 2016 | 27114558 |
| use of a probabilistic motif search to identify histidine phosphotransfer domain-containing proteins. | the wealth of newly obtained proteomic information affords researchers the possibility of searching for proteins of a given structure or function. here we describe a general method for the detection of a protein domain of interest in any species for which a complete proteome exists. in particular, we apply this approach to identify histidine phosphotransfer (hpt) domain-containing proteins across a range of eukaryotic species. from the sequences of known hpt domains, we created an amino acid occ ... | 2016 | 26751210 |
| the sandfly lutzomyia longipalpis ll5 embryonic cell line has active toll and imd pathways and shows immune responses to bacteria, yeast and leishmania. | lutzomyia longipalpis is the main vector of visceral leishmaniasis in latin america. sandfly immune responses are poorly understood. in previous work we showed that these vector insects respond to bacterial infections by modulating a defensin gene expression and activate the imd pathway in response to leishmania infection. aspects of innate immune pathways in insects (including mosquito vectors of human diseases) have been revealed by studying insect cell lines, and we have previously demonstrat ... | 2016 | 27098567 |
| emerging role of lipid droplets in aedes aegypti immune response against bacteria and dengue virus. | in mammals, lipid droplets (lds) are ubiquitous organelles that modulate immune and inflammatory responses through the production of lipid mediators. in insects, it is unknown whether lds play any role during the development of immune responses. we show that aedes aegypti aag2 cells - an immune responsive cell lineage - accumulates lds when challenged with enterobacter cloacae, sindbis, and dengue viruses. microarray analysis of aag2 challenged with e.cloacae or infected with dengue virus reveal ... | 2016 | 26887863 |
| mass spectrometry analysis and transcriptome sequencing reveal glowing squid crystal proteins are in the same superfamily as firefly luciferase. | the japanese firefly squid hotaru-ika (watasenia scintillans) produces intense blue light from photophores at the tips of two arms. these photophores are densely packed with protein microcrystals that catalyse the bioluminescent reaction using atp and the substrate coelenterazine disulfate. the squid is the only organism known to produce light using protein crystals. we extracted microcrystals from arm tip photophores and identified the constituent proteins using mass spectrometry and transcript ... | 2016 | 27279452 |
| tetrapyrroles as endogenous tspo ligands in eukaryotes and prokaryotes: comparisons with synthetic ligands. | the 18 kda translocator protein (tspo) is highly 0conserved in eukaryotes and prokaryotes. since its discovery in 1977, numerous studies established the tspo's importance for life essential functions. for these studies, synthetic tspo ligands typically are applied. tetrapyrroles present endogenous ligands for the tspo. tetrapyrroles are also evolutionarily conserved and regulate multiple functions. tspo and tetrapyrroles regulate each other. in animals tspo-tetrapyrrole interactions range from e ... | 2016 | 27271616 |
| novel nad+-farnesal dehydrogenase from polygonum minus leaves. purification and characterization of enzyme in juvenile hormone iii biosynthetic pathway in plant. | juvenile hormone iii is of great concern due to negative effects on major developmental and reproductive maturation in insect pests. thus, the elucidation of enzymes involved jh iii biosynthetic pathway has become increasing important in recent years. one of the enzymes in the jh iii biosynthetic pathway that remains to be isolated and characterized is farnesal dehydrogenase, an enzyme responsible to catalyze the oxidation of farnesal into farnesoic acid. a novel nad+-farnesal dehydrogenase of p ... | 2016 | 27560927 |
| functional genomic analyses of enterobacter, anopheles and plasmodium reciprocal interactions that impact vector competence. | malaria exerts a tremendous socioeconomic impact worldwide despite current control efforts, and novel disease transmission-blocking strategies are urgently needed. the enterobacter bacterium esp_z, which is naturally harboured in the mosquito midgut, can inhibit the development of plasmodium parasites prior to their invasion of the midgut epithelium through a mechanism that involves oxidative stress. here, a multifaceted approach is used to study the tripartite interactions between the mosquito, ... | 2016 | 27549662 |
| depletion of alveolar macrophages does not prevent hantavirus disease pathogenesis in golden syrian hamsters. | andes virus (andv) is associated with a lethal vascular leak syndrome in humans termed hantavirus pulmonary syndrome (hps). the mechanism for the massive vascular leakage associated with hps is poorly understood; however, dysregulation of components of the immune response is often suggested as a possible cause. alveolar macrophages are found in the alveoli of the lung and represent the first line of defense to many airborne pathogens. to determine whether alveolar macrophages play a role in hps ... | 2016 | 27099308 |
| analysis of protein composition and bioactivity of neoponera villosa venom (hymenoptera: formicidae). | ants cause a series of accidents involving humans. such accidents generate different reactions in the body, ranging from a mild irritation at the bite site to anaphylactic shock, and these reactions depend on the mechanism of action of the venom. the study of animal venom is a science known as venomics. through venomics, the composition of the venom of several ant species has already been characterized and their biological activities described. thus, the aim of this study was to evaluate the pro ... | 2016 | 27110765 |
| tiger mosquito - generated devastation in the biosphere!!! | 2016 | 27041891 | |
| how to predict molecular interactions between species? | organisms constantly interact with other species through physical contact which leads to changes on the molecular level, for example the transcriptome. these changes can be monitored for all genes, with the help of high-throughput experiments such as rna-seq or microarrays. the adaptation of the gene expression to environmental changes within cells is mediated through complex gene regulatory networks. often, our knowledge of these networks is incomplete. network inference predicts gene regulator ... | 2016 | 27065992 |
| transfer of dna from bacteria to eukaryotes. | historically, the members of the agrobacterium genus have been considered the only bacterial species naturally able to transfer and integrate dna into the genomes of their eukaryotic hosts. yet, increasing evidence suggests that this ability to genetically transform eukaryotic host cells might be more widespread in the bacterial world. indeed, analyses of accumulating genomic data reveal cases of horizontal gene transfer from bacteria to eukaryotes and suggest that it represents a significant fo ... | 2016 | 27406565 |
| horizontal transfer of β-carbonic anhydrase genes from prokaryotes to protozoans, insects, and nematodes. | horizontal gene transfer (hgt) is a movement of genetic information occurring outside of normal mating activities. it is especially common between prokaryotic endosymbionts and their protozoan, insect, and nematode hosts. although beta carbonic anhydrase (β-ca) plays a crucial role in metabolic functions of many living organisms, the origin of β-ca genes in eukaryotic species remains unclear. | 2016 | 26983858 |
| evidence for the induction of key components of the notch signaling pathway via deltamethrin and azamethiphos treatment in the sea louse caligus rogercresseyi. | the extensive use of organophosphates and pyrethroids in the aquaculture industry has negatively impacted parasite sensitivity to the delousing effects of these antiparasitics, especially among sea lice species. the notch signaling pathway is a positive regulator of abc transporter subfamily c expression and plays a key role in the generation and modulation of pesticide resistance. however, little is known about the molecular mechanisms behind pesticide resistance, partly due to the lack of geno ... | 2016 | 27187362 |
| wolbachia blocks viral genome replication early in infection without a transcriptional response by the endosymbiont or host small rna pathways. | the intracellular endosymbiotic bacterium wolbachia can protect insects against viral infection, and is being introduced into mosquito populations in the wild to block the transmission of arboviruses that infect humans and are a major public health concern. to investigate the mechanisms underlying this antiviral protection, we have developed a new model system combining wolbachia-infected drosophila melanogaster cell culture with the model mosquito-borne semliki forest virus (sfv; togaviridae, a ... | 2016 | 27089431 |
| zika virus strains potentially display different infectious profiles in human neural cells. | the recent zika virus (zikv) epidemic has highlighted the poor knowledge on its physiopathology. recent studies showed that zikv of the asian lineage, responsible for this international outbreak, causes neuropathology in vitro and in vivo. however, two african lineages exist and the virus is currently found circulating in africa. the original african strain was also suggested to be neurovirulent but its laboratory usage has been criticized due to its multiple passages. in this study, we compared ... | 2016 | 27688094 |
| pirna pathway is not required for antiviral defense in drosophila melanogaster. | since its discovery, rna interference has been identified as involved in many different cellular processes, and as a natural antiviral response in plants, nematodes, and insects. in insects, the small interfering rna (sirna) pathway is the major antiviral response. in recent years, the piwi-interacting rna (pirna) pathway also has been implicated in antiviral defense in mosquitoes infected with arboviruses. using drosophila melanogaster and an array of viruses that infect the fruit fly acutely o ... | 2016 | 27357659 |
| yellow fever virus capsid protein is a potent suppressor of rna silencing that binds double-stranded rna. | mosquito-borne flaviviruses, including yellow fever virus (yfv), zika virus (zikv), and west nile virus (wnv), profoundly affect human health. the successful transmission of these viruses to a human host depends on the pathogen's ability to overcome a potentially sterilizing immune response in the vector mosquito. similar to other invertebrate animals and plants, the mosquito's rna silencing pathway comprises its primary antiviral defense. although a diverse range of plant and insect viruses has ... | 2016 | 27849599 |
| genome investigations of vector competence in aedes aegypti to inform novel arbovirus disease control approaches. | dengue (denv), yellow fever, chikungunya, and zika virus transmission to humans by a mosquito host is confounded by both intrinsic and extrinsic variables. besides virulence factors of the individual arboviruses, likelihood of virus transmission is subject to variability in the genome of the primary mosquito vector, aedes aegypti. the "vectorial capacity" of a. aegypti varies depending upon its density, biting rate, and survival rate, as well as its intrinsic ability to acquire, host and transmi ... | 2016 | 27809220 |
| climate change influences on the global potential distribution of the mosquito culex quinquefasciatus, vector of west nile virus and lymphatic filariasis. | rapid emergence of most vector-borne diseases (vbds) may be associated with range expansion of vector populations. culex quinquefasciatus say 1823 is a potential vector of west nile virus, saint louis encephalitis virus, and lymphatic filariasis. we estimated the potential distribution of cx. quinquefasciatus under both current and future climate conditions. the present potential distribution of cx. quinquefasciatus showed high suitability across low-latitude parts of the world, reflecting the c ... | 2016 | 27695107 |
| morphological abnormalities and cell death in the asian citrus psyllid (diaphorina citri) midgut associated with candidatus liberibacter asiaticus. | candidatus liberibacter asiaticus (clas) is a phloem-limited, gram-negative, fastidious bacterium that is associated with the development of citrus greening disease, also known as huanglongbing (hlb). clas is transmitted by the asian citrus psyllid (acp) diaphorina citri, in a circulative manner. two major barriers to transmission within the insect are the midgut and the salivary glands. we performed a thorough microscopic analysis within the insect midgut following exposure to clas-infected cit ... | 2016 | 27630042 |
| the strica homolog aacasps16 is involved in apoptosis in the yellow fever vector, aedes albopictus. | caspases are a family of cysteine proteases playing essential roles during apoptosis. seven caspases identified in drosophila were dronc, dredd, strica, dcp-1, decay, drice and damm. among them, strica is an insect-specific caspase containing a long serine- and threonine- rich prodomain, of which function is not yet well studied. here we identified a homolog of strica from aedes albopictus, named as aacasps16. aacasps16 encoded a protein containing a putative serine- and threonine-rich prodomain ... | 2016 | 27351972 |
| mosquito saliva increases endothelial permeability in the skin, immune cell migration, and dengue pathogenesis during antibody-dependent enhancement. | dengue remains the most prevalent arthropod-borne viral disease in humans. while probing for blood vessels, aedes aegypti and ae. albopictus mosquitoes transmit the four serotypes of dengue virus (denv1-4) by injecting virus-containing saliva into the skin. even though arthropod saliva is known to facilitate transmission and modulate host responses to other pathogens, the full impact of mosquito saliva on dengue pathogenesis is still not well understood. inoculating mice lacking the interferon-α ... | 2016 | 27310141 |
| a glance at subgenomic flavivirus rnas and micrornas in flavivirus infections. | the family flaviviridae comprises a wide variety of viruses that are distributed worldwide, some of which are associated with high rates of morbidity and mortality. there are neither vaccines nor antivirals for most flavivirus infections, reinforcing the importance of research on different aspects of the viral life cycle. during infection, cytoplasmic accumulation of rna fragments mainly originating from the 3' utrs, which have been designated subgenomic flavivirus rnas (sfrnas), has been detect ... | 2016 | 27233361 |
| infection pattern and transmission potential of chikungunya virus in two new world laboratory-adapted aedes aegypti strains. | chikungunya virus (chikv) is an emerging mosquito-borne virus belonging to the togaviridae, which is transmitted to humans by aedes aegypti and ae. albopictus. we describe the infection pattern of chikv in two new world ae. aegypti strains, hwe and orl. both mosquito strains were susceptible to the virus but showed different infection patterns in midguts and salivary glands. even though acquisition of a bloodmeal showed moderate levels of apoptosis in midgut tissue, there was no obvious addition ... | 2016 | 27102548 |
| endogenously-expressed nh2-terminus of circumsporozoite protein interferes with sporozoite invasion of mosquito salivary glands. | the circumsporozoite protein is the most abundant polypeptide expressed by sporozoites, the malaria parasite stage capable of infecting humans. sporozoite invasion of mosquito salivary glands prior to transmission is likely mediated by a receptor/ligand-like interaction of the parasites with the target tissues, and the amino (nh2)-terminal portion of csp is involved in this interaction but not the tsr region on the carboxyl (c)-terminus. peptides based on the nh2-terminal domain could compete wi ... | 2016 | 26964736 |
| genomic approaches for understanding dengue: insights from the virus, vector, and host. | the incidence and geographic range of dengue have increased dramatically in recent decades. climate change, rapid urbanization and increased global travel have facilitated the spread of both efficient mosquito vectors and the four dengue virus serotypes between population centers. at the same time, significant advances in genomics approaches have provided insights into host-pathogen interactions, immunogenetics, and viral evolution in both humans and mosquitoes. here, we review these advances an ... | 2016 | 26931545 |
| small rna profiling in dengue virus 2-infected aedes mosquito cells reveals viral pirnas and novel host mirnas. | in aedes mosquitoes, infections with arthropod-borne viruses (arboviruses) trigger or modulate the expression of various classes of viral and host-derived small rnas, including small interfering rnas (sirnas), piwi interacting rnas (pirnas), and micrornas (mirnas). viral sirnas are at the core of the antiviral rna interference machinery, one of the key pathways that limit virus replication in invertebrates. besides sirnas, aedes mosquitoes and cells derived from these insects produce arbovirus-d ... | 2016 | 26914027 |
| transstadial effects of bti on traits of aedes aegypti and infection with dengue virus. | most mosquito control efforts are primarily focused on reducing the adult population size mediated by reductions in the larval population, which should lower risk of disease transmission. although the aim of larviciding is to reduce larval abundance and thus recruitment of adults, nonlethal effects on adults are possible, including transstadial effects on phenotypes of adults such as survival and pathogen infection and transmission. in addition, the mortality induced by control efforts may act i ... | 2016 | 26871951 |
| inhibition of chikungunya virus replication by 1-[(2-methylbenzimidazol-1-yl) methyl]-2-oxo-indolin-3-ylidene] amino] thiourea(mbzm-n-ibt). | chikungunya virus (chikv) infection is one of the most challenging human arboviral infections with global significance and without any specific antiviral. in this investigation, 1-[(2-methylbenzimidazol-1-yl) methyl]-2-oxo-indolin-3-ylidene] amino] thiourea (mbzm-n-ibt) was synthesised as a molecular hybrid of 2-methyl benzimidazole and isatin-β-thiosemicarbazone and its anti-chikv property was evaluated. the release of infectious virus particles was calculated by plaque assay, expression profil ... | 2016 | 26843462 |
| apoptosis, autophagy and unfolded protein response pathways in arbovirus replication and pathogenesis. | arboviruses are pathogens that widely affect the health of people in different communities around the world. recently, a few successful approaches toward production of effective vaccines against some of these pathogens have been developed, but treatment and prevention of the resulting diseases remain a major health and research concern. the arbovirus infection and replication processes are complex, and many factors are involved in their regulation. apoptosis, autophagy and the unfolded protein r ... | 2016 | 26781343 |
| viral metagenomics on blood-feeding arthropods as a tool for human disease surveillance. | surveillance and monitoring of viral pathogens circulating in humans and wildlife, together with the identification of emerging infectious diseases (eids), are critical for the prediction of future disease outbreaks and epidemics at an early stage. it is advisable to sample a broad range of vertebrates and invertebrates at different temporospatial levels on a regular basis to detect possible candidate viruses at their natural source. however, virus surveillance systems can be expensive, costly i ... | 2016 | 27775568 |
| an updated checklist of mosquito species (diptera: culicidae) from madagascar. | an updated checklist of 235 mosquito species from madagascar is presented. the number of species has increased considerably compared to previous checklists, particularly the last published in 2003 (178 species). this annotated checklist provides concise information on endemism, taxonomic position, developmental stages, larval habitats, distribution, behavior, and vector-borne diseases potentially transmitted. the 235 species belong to 14 genera: aedeomyia (3 species), aedes (35 species), anophel ... | 2016 | 27101839 |
| the insect-specific palm creek virus modulates west nile virus infection in and transmission by australian mosquitoes. | insect-specific viruses do not replicate in vertebrate cells, but persist in mosquito populations and are highly prevalent in nature. these viruses may naturally regulate the transmission of pathogenic vertebrate-infecting arboviruses in co-infected mosquitoes. following the isolation of the first australian insect-specific flavivirus (isf), palm creek virus (pcv), we investigated routes of infection and transmission of this virus in key australian arbovirus vectors and its impact on replication ... | 2016 | 27457250 |
| development and validation of a quantitative, one-step, multiplex, real-time reverse transcriptase pcr assay for detection of dengue and chikungunya viruses. | dengue virus (denv) and chikungunya virus (chikv) are important human pathogens with common transmission vectors and similar clinical presentations. patient care may be impacted by the misdiagnosis of denv and chikv in areas where both viruses cocirculate. in this study, we have developed and validated a one-step multiplex reverse transcriptase pcr (rt-pcr) to simultaneously detect, quantify, and differentiate between four denv serotypes (pan-denv) and chikungunya virus. the assay uses taqman te ... | 2016 | 27098955 |
| vector competence of aedes aegypti and aedes polynesiensis populations from french polynesia for chikungunya virus. | from october 2014 to march 2015, french polynesia experienced for the first time a chikungunya outbreak. two aedes mosquitoes may have contributed to chikungunya virus (chikv) transmission in french polynesia: the worldwide distributed ae. aegypti and the polynesian islands-endemic ae. polynesiensis mosquito. | 2016 | 27144888 |
| vertical transmission of indian ocean lineage of chikungunya virus in aedes aegypti and aedes albopictus mosquitoes. | the re-emergence of chikungunya (chik) fever in thailand has been caused by a novel lineage of chikungunya virus (chikv) termed the indian ocean lineage (iol). the aedes albopictus mosquito is thought to be a primary vector of chik fever in thailand, whereas ae. aegypti acts as a secondary vector of the virus. the vertical transmission is believed to be a primary means to maintain chikv in nature and may be associated with an increased risk of outbreak. therefore, the goal of this study was to a ... | 2016 | 27108077 |
| membrane feeding of dengue patient's blood as a substitute for direct skin feeding in studying aedes-dengue virus interaction. | understanding the interaction between aedes vectors and dengue viruses (denv) has significant implications in determining the transmission dynamics of dengue. the absence of an animal model and ethical concerns regarding direct feeding of mosquitoes on patients has resulted in most infection studies using blood meals spiked with laboratory-cultured denv. data obtained from such studies may not reflect the natural human-mosquito transmission scenario. this study explored the potential of using me ... | 2016 | 27083158 |
| rapid detection strategies for the global threat of zika virus: current state, new hypotheses, and limitations. | the current scenario regarding the widespread zika virus (zikv) has resulted in numerous diagnostic studies, specifically in south america and in locations where there is frequent entry of travelers returning from zikv-affected areas, including pregnant women with or without clinical symptoms of zikv infection. the world health organization, who, announced that millions of cases of zikv are likely to occur in the usa in the near future. this situation has created an alarming public health emerge ... | 2016 | 27822207 |
| characterization of a novel murine model to study zika virus. | the mosquito-borne zika virus (zikv) is responsible for an explosive ongoing outbreak of febrile illness across the americas. zikv was previously thought to cause only a mild, flu-like illness, but during the current outbreak, an association with guillain-barré syndrome and microcephaly in neonates has been detected. a previous study showed that zikv requires murine adaptation to generate reproducible murine disease. in our study, a low-passage cambodian isolate caused disease and mortality in m ... | 2016 | 27022155 |
| epidemiology of emergent madariaga encephalitis in a region with endemic venezuelan equine encephalitis: initial host studies and human cross-sectional study in darien, panama. | neurotropic arboviral infections are an important cause of encephalitis. a zoonotic, vector-borne alphavirus, madariaga virus (madv; formerly known as south american eastern equine encephalitis virus), caused its first documented human outbreak in 2010 in darien, panama, where the genetically similar venezuelan equine encephalitis virus (veev) is endemic. we report the results of a seroprevalence survey of animals and humans, illustrating contrasting features of madv and veev ecology and epidemi ... | 2016 | 27101567 |
| the role of the membrane in the structure and biophysical robustness of the dengue virion envelope. | the dengue virion is surrounded by an envelope of membrane proteins surrounding a lipid bilayer. we have combined the cryoelectron microscopy structures of the membrane proteins (pdb: 3j27) with a lipid bilayer whose composition is based on lipidomics data for insect cell membranes, to obtain a near-atomic resolution computational model of the envelope of the dengue virion. a coarse-grained molecular dynamics simulation on the microsecond timescale enables analysis of key biophysical properties ... | 2016 | 26833387 |
| intra-epidemic evolutionary dynamics of a dengue virus type 1 population reveal mutant spectra that correlate with disease transmission. | dengue virus (denv) is currently the most prevalent mosquito-borne viral pathogen. denvs naturally exist as highly heterogeneous populations. even though the descriptions on denv diversity are plentiful, only a few studies have narrated the dynamics of intra-epidemic virus diversity at a fine scale. such accounts are important to decipher the reciprocal relationship between viral evolutionary dynamics and disease transmission that shape dengue epidemiology. in the current study, we present a mic ... | 2016 | 26940650 |
| zika virus and its implication in transfusion safety. | 2016 | 26969782 | |
| transcriptome analysis of the midgut of the chinese oak silkworm antheraea pernyi infected with antheraea pernyi nucleopolyhedrovirus. | the antheraea pernyi nucleopolyhedrovirus (apnpv) is an exclusive pathogen of a. pernyi. the intense interactions between apnpv and a. pernyi cause a series of physiological and pathological changes to a. pernyi. however, no detailed report exists regarding the molecular mechanisms underlying the interactions between apnpv and a. pernyi. in this study, four cdna libraries of the a. pernyi midgut, including two apnpv-infected groups and two control groups, were constructed for transcriptomic anal ... | 2016 | 27820844 |
| prolonged shedding of chikungunya virus in semen and urine: a new perspective for diagnosis and implications for transmission. | we report the presence of chikungunya (chikv) rna in the blood, urine and semen during the acute phase of the disease in an adult with a dual infection with dengue virus type 3. the patient, a 25 yr-old man from salvador, brazil, reported a 6-day duration of high fever, arthralgia, myalgia, headache and photophobia.blood and semen specimens were positive for chikv in the first collected samples; semen and urine specimens were positive for chikv after 30 days of symptoms onset. denv-3 rna was pos ... | 2016 | 27882301 |
| genome sequence of dengue virus 3 from the pythium insidiosum transcriptomes. | 2016 | 27379056 | |
| zika virus: new clinical syndromes and its emergence in the western hemisphere. | zika virus (zikv) had remained a relatively obscure flavivirus until a recent series of outbreaks accompanied by unexpectedly severe clinical complications brought this virus into the spotlight as causing an infection of global public health concern. in this review, we discuss the history and epidemiology of zikv infection, recent outbreaks in oceania and the emergence of zikv in the western hemisphere, newly ascribed complications of zikv infection, including guillain-barré syndrome and microce ... | 2016 | 26962217 |
| the global spread of zika virus: is public and media concern justified in regions currently unaffected? | zika virus, an aedes mosquito-borne flavivirus, is fast becoming a worldwide public health concern following its suspected association with over 4000 recent cases of microcephaly among newborn infants in brazil. | 2016 | 27093860 |
| zika virus: the latest newcomer. | since the beginning of this century, humanity has been facing a new emerging, or re-emerging, virus threat almost every year: west nile, influenza a, avian flu, dengue, chikungunya, sars, mers, ebola, and now zika, the latest newcomer. zika virus (zikv), a flavivirus transmitted by aedes mosquitoes, was identified in 1947 in a sentinel monkey in uganda, and later on in humans in nigeria. the virus was mainly confined to the african continent until it was detected in south-east asia the 1980's, t ... | 2016 | 27148186 |
| clinical and epidemiological characterization of laboratory-confirmed autochthonous cases of zika virus disease in mexico. | since 2014, autochthonous circulation of zika virus (zikv) in the americas was detected (easter island, chile). in may 2015, brazil confirmed autochthonous --transmission and in october of that year colombia reported their first cases. now more than 52 countries have reported cases, including mexico. to deal with this contingency in mexico, several surveillance systems, in addition to systems for vector-borne diseases were strengthened with the participation of all health institutions. also, th ... | 2016 | 27158557 |
| computational approach towards exploring potential anti-chikungunya activity of selected flavonoids. | chikungunya virus (chikv) is a mosquito-borne alphavirus that causes chikungunya infection in humans. despite the widespread distribution of chikv, no antiviral medication or vaccine is available against this virus. therefore, it is crucial to find an effective compound to combat chikv. we aimed to predict the possible interactions between non-structural protein 3 (nsp) of chikv as one of the most important viral elements in chikv intracellular replication and 3 potential flavonoids using a comp ... | 2016 | 27071308 |
| zika virus infections imported from brazil to portugal, 2015. | zika virus is an emerging arbovirus transmitted by aedes sp. mosquitoes like the dengue and chikungunya viruses. zika virus was until recently considered a mild pathogenic mosquito-borne flavivirus with very few reported benign human infections. in 2007, an epidemic in micronesia initiated the turnover in the epidemiological history of zika virus and more recently, the potential association with congenital microcephaly cases in brazil 2015, still under investigation, led the world health organiz ... | 2016 | 27134823 |
| estimating risks of importation and local transmission of zika virus infection. | background. an international spread of zika virus (zikv) infection has attracted global attention. zikv is conveyed by a mosquito vector, aedes species, which also acts as the vector species of dengue and chikungunya viruses. methods. arrival time of zikv importation (i.e., the time at which the first imported case was diagnosed) in each imported country was collected from publicly available data sources. employing a survival analysis model in which the hazard is an inverse function of the effec ... | 2016 | 27069825 |
| identify-isolate-inform: a tool for initial detection and management of zika virus patients in the emergency department. | first isolated in 1947 from a monkey in the zika forest in uganda, and from mosquitoes in the same forest the following year, zika virus has gained international attention due to concerns for infection in pregnant women potentially causing fetal microcephaly. more than one million people have been infected since the appearance of the virus in brazil in 2015. approximately 80% of infected patients are asymptomatic. an association with microcephaly and other birth defects as well as guillain-barre ... | 2016 | 27330653 |
| recombinant dengue 2 virus ns3 helicase protein enhances antibody and t-cell response of purified inactivated vaccine. | dengue virus purified inactivated vaccines (piv) are highly immunogenic and protective over the short term, but may be poor at inducing cell-mediated immune responses and long-term protection. the dengue nonstructural protein 3 (ns3) is considered the main target for t-cell responses during viral infection. the amino (n)-terminal protease and the carboxy (c)-terminal helicase domains of denv-2 ns3 were expressed in e. coli and analyzed for their immune-potentiating capacity. mice were immunized ... | 2016 | 27035715 |
| zika virus: diagnostics for an emerging pandemic threat. | zika virus (zikv) is anaedesmosquito-borne flavivirus that emerged in brazil in 2015 and then rapidly spread throughout the tropical and subtropical americas. based on clinical criteria alone, zikv cannot be reliably distinguished from infections with other pathogens that cause an undifferentiated systemic febrile illness, including infections with two common arboviruses, dengue virus and chikungunya virus. this minireview details the methods that are available to diagnose zikv infection. | 2016 | 26888897 |
| maternal antibody-mediated disease enhancement in type i interferon-deficient mice leads to lethal disease associated with liver damage. | epidemiological studies have reported that most of the severe dengue cases occur upon a secondary heterologous infection. furthermore, babies born to dengue immune mothers are at greater risk of developing severe disease upon primary infection with a heterologous or homologous dengue virus (denv) serotype when maternal antibodies reach sub-neutralizing concentrations. these observations have been explained by the antibody mediated disease enhancement (ade) phenomenon whereby heterologous antibod ... | 2016 | 27007501 |
| nucleotide composition of the zika virus rna genome and its codon usage. | rna viruses have genomes with a distinct nucleotide composition and codon usage. we present the global characteristics of the rna genome of zika virus (zikv), an emerging pathogen within the flavivirus genus. zikv was first isolated in 1947 in uganda, caused a widespread epidemic in south and central america and the caribbean in 2015 and has recently been associated with microcephaly in newborns. | 2016 | 27278486 |
| pathogenesis of zika virus-associated embryopathy. | a strong causal association has become evident between zika virus (zikv) infection during pregnancy and the occurrence of fetal growth restriction, microcephaly and eye defects. circumstantial evidence is presented in this paper in support of the hypothesis that these effects, as well as the guillain-barré syndrome, are due to an endogenous form of hypervitaminosis a resulting from zikv infection-induced damage to the liver and the spillage of stored vitamin a compounds ("retinoids") into the ma ... | 2016 | 27403405 |
| long-term safety and immunogenicity of a tetravalent live-attenuated dengue vaccine and evaluation of a booster dose administered to healthy thai children. | we evaluated the safety and immunogenicity of two doses of a live-attenuated, tetravalent dengue virus vaccine (f17/pre formulation) and a booster dose in a dengue endemic setting in two studies. seven children (7- to 8-year-olds) were followed for 1 year after dose 2 and then given a booster dose (f17/pre formulation), and followed for four more years (child study). in the infant study, 49 2-year-olds, vaccinated as infants, were followed for approximately 3.5 years after dose 2 and then given ... | 2016 | 27022153 |
| scoping review of the zika virus literature. | the global primary literature on zika virus (zikv) (n = 233 studies and reports, up to march 1, 2016) has been compiled using a scoping review methodology to systematically identify and characterise the literature underpinning this broad topic using methods that are documented, updateable and reproducible. our results indicate that more than half the primary literature on zikv has been published since 2011. the articles mainly covered three topic categories: epidemiology of zikv (surveillance an ... | 2016 | 27244249 |
| quantifying the epidemiological impact of vector control on dengue. | 2016 | 27227829 | |
| a pan-dengue virus reverse transcription-insulated isothermal pcr assay intended for point-of-need diagnosis of dengue virus infection by use of the pockit nucleic acid analyzer. | dengue virus (denv) infection is considered a major public health problem in developing tropical countries where the virus is endemic and continues to cause major disease outbreaks every year. here, we describe the development of a novel, inexpensive, and user-friendly diagnostic assay based on a reverse transcription-insulated isothermal pcr (rt-iipcr) method for the detection of all four serotypes of denv in clinical samples. the diagnostic performance of the newly established pan-denv rt-iipc ... | 2016 | 27030492 |
| determinants of arbovirus vertical transmission in mosquitoes. | vertical transmission (vt) and horizontal transmission (ht) of pathogens refer to parental and non-parental chains of host-to-host transmission. combining ht with vt enlarges considerably the range of ecological conditions in which a pathogen can persist, but the factors governing the relative frequency of each transmission mode are poorly understood for pathogens with mixed-mode transmission. elucidating these factors is particularly important for understanding the epidemiology of arthropod-bor ... | 2016 | 27171170 |
| physicians, primary caregivers and topical repellent: all under-utilised resources in stopping dengue virus transmission in affected households. | primary health care facilities frequently manage dengue cases on an ambulatory basis for the duration of the patient's illness. there is a great opportunity for specific messaging, aimed to reduce dengue virus (denv) transmission in and around the home, to be directly targeted toward this high-risk ambulatory patient group, as part of an integrated approach to dengue management. the extent however, to which physicians understand, and can themselves effectively communicate strategies to stop foca ... | 2016 | 27164168 |
| coeloconic sensilla on the antennae of the yellow fever mosquito, aedes aegypti (l.). | 2016 | 5026452 | |
| functional analysis of the abcs of eye color in helicoverpa armigera with crispr/cas9-induced mutations. | many insect pigments are localized in subcellular pigment granules, and transport of pigment precursors from the cytoplasm is accomplished by abc proteins. drosophila melanogaster has three half-transporter genes (white, scarlet, and brown, all affecting eye pigments) and bombyx mori has a fourth (ok). the white, brown, scarlet and ok proteins each have one transmembrane and one cytoplasmic domain and they heterodimerize to form functional transporters with different substrate specificities. we ... | 2017 | 28053351 |
| efficient method for establishing f1 progeny from wild populations of anopheles mosquitoes. | the changing malaria situation in madagascar requires additional knowledge on the physiology and behaviour of local mosquito vectors. however, the absence of established colonies for several anopheline species present in madagascar constitutes a limiting factor. to avoid labour intensive work and uncertainty for success of establishing anopheles colonies from malagasy species, field collections of blood-fed females and in-tube forced oviposition were combined to reliably produce large numbers of ... | 2017 | 28069024 |
| commensal viruses of mosquitoes: host restriction, transmission, and interaction with arboviral pathogens. | recent advances in virus detection strategies and deep sequencing technologies have enabled the identification of a multitude of new viruses that persistently infect mosquitoes but do not infect vertebrates. these are usually referred to as insect-specific viruses (isvs). these novel viruses have generated considerable interest in their modes of transmission, persistence in mosquito populations, the mechanisms that restrict their host range to mosquitoes, and their interactions with pathogens tr ... | 2017 | 28096646 |
| persistent zika virus detection in semen in a traveler returning to the united kingdom from brazil, 2016. | zika virus is normally transmitted by mosquitos, but cases of sexual transmission have been reported. we describe a patient with symptomatic zika virus infection in whom the virus was detected in semen for 92 days. our findings support recommendations for 6 months of barrier contraceptive use after symptomatic zika virus infection. | 2017 | 27748650 |
| epidemic outbreak of chikungunya in two neighboring towns in the colombian caribbean: a survival analysis. | the first autochthonous chikungunya virus transmission in colombia was reported in september 2014. three months later, every town in the caribbean region was affected, including the bordering towns of ovejas and corozal, in the department of sucre. the objective of the study was to analyze and compare the temporal dynamics of the outbreak of chikungunya in two towns of the department of sucre. | 2017 | 28074128 |
| synthesis and insecticidal activity of acridone derivatives to aedes aegypti and culex quinquefasciatus larvae and non-target aquatic species. | a serious mosquito borne yellow fever is one of the grave diseases which affect the major population. since there is no specific treatment for yellow fever, there is a necessity to develop an effective agent. the series of acridinone analogues 3 to 5 were synthesized with help of non-conventional microwave heating and confirmed by respective spectral characterization. 5c and 3b showed highest activity to kill 90% of larvae against a. aegypti and c. quinquefasciatus, respectively. also the active ... | 2017 | 28059104 |
| discovery of flavivirus-derived endogenous viral elements in anopheles mosquito genomes supports the existence of anopheles-associated insect-specific flaviviruses. | the flavivirus genus encompasses several arboviruses of public health significance such as dengue, yellow fever, and zika viruses. it also includes insect-specific flaviviruses (isfs) that are only capable of infecting insect hosts. the vast majority of mosquito-infecting flaviviruses have been associated with mosquito species of the aedes and culex genera in the culicinae subfamily, which also includes most arbovirus vectors. mosquitoes of the anophelinae subfamily are not considered significan ... | 2017 | 28078104 |
| enhancement of mosquito trapping efficiency by using pulse width modulated light emitting diodes. | in this study, a light-driving bug zapper is presented for well controlling the diseases brought by insects, such as mosquitoes. in order to have the device efficient to trap the insect pests in off-grid areas, pulse width modulated light emitting diodes (pwm-led) combined with a solar power module are proposed and implemented. with specific pwm electric signals to drive the led, it is found that no matter what the ability of catching insects or the consumed power efficiency can be enhanced thus ... | 2017 | 28059148 |
| evaluations of dual attractant toxic sugar baits for surveillance and control of aedes aegypti and aedes albopictus in florida. | dual attractant toxic sugar baits (d-atsb) containing two host kairomones, l-lactic (la) and 1-octen-3-ol (o), and fruit-based attractants were evaluated through olfactory, consumption and mortality, and semi-field experiments to determine if host kairomones could first, enhance attraction of a fruit-based (attractant) toxic sugar bait (atsb), and second, increase the efficacy of a fruit based attractive toxic sugar bait (atsb). | 2017 | 28057066 |
| evaluation of the importance of fever with respect to dengue prognosis according to the 2009 who classification: a retrospective study. | the 2009 revised world health organization (who) guidelines for dengue describe fever as the core symptom. accordingly, the diagnosis of non-febrile patients is complicated. the aim of this study was to evaluate the importance of fever in patients with dengue according to the 2009 revised who classification. | 2017 | 28052760 |
| assessment of synthetic floral-based attractants and sugar baits to capture male and female aedes aegypti (diptera: culicidae). | the viruses transmitted by aedes aegypti, including dengue and zika viruses, are rapidly expanding in geographic range and as a threat to public health. in response, control programs are increasingly turning to the use of sterile insect techniques resulting in a need to trap male ae. aegypti to monitor the efficacy of the intervention. however, there is a lack of effective and cheap methods for trapping males. thus, we attempted to exploit the physiological need to obtain energy from sugar feedi ... | 2017 | 28095875 |
| identification of functional enolase genes of the silkworm bombyx mori from public databases with a combination of dry and wet bench processes. | various insect species have been added to genomic databases over the years. thus, researchers can easily obtain online genomic information on invertebrates and insects. however, many incorrectly annotated genes are included in these databases, which can prevent the correct interpretation of subsequent functional analyses. to address this problem, we used a combination of dry and wet bench processes to select functional genes from public databases. | 2017 | 28086791 |
| application of the analytic hierarchy approach to the risk assessment of zika virus disease transmission in guangdong province, china. | an international spread of zika virus (zikv) infection has attracted global attention in 2015. the infection also affected guangdong province, which is located in southern china. multiple factors, including frequent communication with south america and southeast asia, suitable climate (sub-tropical) for the habitat of aedes species, may increase the risk of zikv disease transmission in this region. | 2017 | 28086897 |
| functional characterization of the active mutator-like transposable element, muta1 from the mosquito aedes aegypti. | mutator-like transposable elements (mules) are widespread with members in fungi, plants, and animals. most of the research on the mule superfamily has focused on plant mules where they were discovered and where some are extremely active and have significant impact on genome structure. the maize mudr element has been widely used as a tool for both forward and reverse genetic studies because of its high transposition rate and preference for targeting genic regions. however, despite being widesprea ... | 2017 | 28096902 |
| juvenile hormone biosynthesis in adult blattella germanica requires nuclear receptors seven-up and ftz-f1. | in insects, the transition from juvenile development to the adult stage is controlled by juvenile hormone (jh) synthesized from the corpora allata (ca) glands. whereas a jh-free period during the last juvenile instar triggers metamorphosis and the end of the growth period, the reappearance of this hormone after the imaginal molt marks the onset of reproductive adulthood. despite the importance of such transition, the regulatory mechanism that controls it remains mostly unknown. here, using the h ... | 2017 | 28074850 |