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genetic variability of the aedes aegypti (diptera: culicidae) mosquito in el salvador, vector of dengue, yellow fever, chikungunya and zika.aedes aegypti is associated with dengue, yellow fever, chikungunya and zika viruses. this vector is widespread in tropical and subtropical areas, and can also occur in temperate areas at higher latitudes. the geographical distribution of ae. aegypti continues to spread due to human activities. this is the first study to examine the population genetic structure of this insect in el salvador, central america.201830547835
susceptible and mcry3a resistant corn rootworm larvae killed by a non-hemolytic bacillus thuringiensis cyt1aa mutant.the western corn rootworm (wcr) diabrotica virgifera virgifera causes substantial damage in corn. genetically modified (gm) plants expressing some bacillus thuringiensis (bt) insecticidal cry proteins efficiently controlled this pest. however, changes in wcr susceptibility to these bt traits have evolved and identification of insecticidal proteins with different modes of action against wcr is necessary. we show here for the first time that cyt1aa from bt exhibits toxicity against wcr besides to ...201830546034
aspergillus terreus (trichocomaceae): a natural, eco-friendly mycoinsecticide for control of malaria, filariasis, dengue vectors and its toxicity assessment against an aquatic model organism artemia nauplii.vector-borne diseases like malaria, filariasis, and dengue are transmitted by mosquitoes and they cause global mortality and morbidity due to an increased resistance against commercial insecticides. the present study was aimed to evaluate the neurobehavioral toxicity, knock-down effect, histopathology, ovicidal, adulticidal, and smoke toxicity effect of aspergillus terreus extract against three mosquito species, namely anopheles stephensi, culex quinquefasciatus, and aedes aegypti (diptera: culi ...201830534070
multispecies transcriptomics data set of brugia malayi, its wolbachia endosymbiont wbm, and aedes aegypti across the b. malayi life cycle.here, we present a comprehensive transcriptomics data set of brugia malayi, its wolbachia endosymbiont wbm, and its vector host. this study samples from 16 stages across the entire b. malayi life cycle, including stage 1 through 4 larvae, adult males and females, embryos, immature microfilariae, and mature microfilariae.201830533772
comparison of landmark- and outline-based geometric morphometrics for discriminating mosquito vectors in ratchaburi province, thailand.it is often challenging to identify mosquito vectors in the field based on morphological features due to their similar morphologies and difficulties in obtaining undamaged samples but is required for their successful control. geometric morphometrics (gm) overcomes this issue by analyzing a suite of traits simultaneously and has the added advantages of being easy to use, low cost, and quick. therefore, this research compared the efficiency and precision of landmark- and outline-based gm technique ...201830533435
dengue outbreaks in hawai'i after wwii - a review of public health response and scientific literature.the four serotypes of dengue virus (denv) cause the most important and common arthropod-borne viral diseases in humans. there have been three major dengue outbreaks in hawai'i since 1946. the most recent and largest outbreak occurred on hawai'i island in 2015-2016. this article reviews the public health response to dengue outbreaks over the period 2001-2016, as well as scientific literature on dengue outbreaks in hawai'i. as summarized in the assessment by the centers for disease control and pre ...201830533283
correction: mosquito pornoscopy: observation and interruption of aedes aegypti copulation to determine female polyandric event and mixed progeny.[this corrects the article doi: 10.1371/journal.pone.0193164.].201830532207
vector competence of biting midges and mosquitoes for shuni virus.shuni virus (shuv) is an orthobunyavirus that belongs to the simbu serogroup. shuv was isolated from diverse species of domesticated animals and wildlife, and is associated with neurological disease, abortions, and congenital malformations. recently, shuv caused outbreaks among ruminants in israel, representing the first incursions outside the african continent. the isolation of shuv from a febrile child in nigeria and seroprevalence among veterinarians in south africa suggests that the virus ma ...201830532189
the composition of midgut bacteria in aedes aegypti (diptera: culicidae) that are naturally susceptible or refractory to dengue viruses.the composition, abundance, and diversity of midgut bacteria in mosquitoes can influence pathogen transmission. we used 16s rrna microbiome profiling to survey midgut microbial diversity in pooled samples of laboratory colonized dengue-refractory, cali-mib, and dengue-susceptible, cali-s aedes aegypti (linnaeus). the 16s rrna sequences from the sugar-fed midguts of adult females clustered to 63 amplicon sequence variants (asvs), primarily from proteobacteria, firmicutes, flavobacteria, and actin ...201830508201
optimization of double-stranded rnai intrathoracic injection method in aedes aegypti.rna interference is widely used to analyze gene functions via phenotypic knockdown of target transcripts in mosquitoes, which transmit numerous mosquito-borne diseases. functional analysis of mosquito genes is indispensable to understand and reduce transmission of mosquito-borne diseases in mosquitoes. intrathoracic injection of double-stranded rna (dsrna) remains the simplest and most customizable method in mosquitoes for functional analysis of the genes of interest. however, achieving consiste ...201830505333
risk prediction system for dengue transmission based on high resolution weather data.dengue is the fastest spreading vector-borne viral disease, resulting in an estimated 390 million infections annually. precise prediction of many attributes related to dengue is still a challenge due to the complex dynamics of the disease. important attributes to predict include: the risk of and risk factors for an infection; infection severity; and the timing and magnitude of outbreaks. in this work, we build a model for predicting the risk of dengue transmission using high-resolution weather d ...201830521550
[costs of an aedes aegypti vector control program in municipalities in colombia: a case study in girón and guadalajara de buga, 2016].the aim of this study was to measure the costs of vector-borne disease control programs at the local level in colombia (2016). a cost analysis was performed for this purpose from the policymaker's perspective in the municipalities (counties) of girón and guadalajara de buga, specifically for the aedes aegypti control program, the principal mosquito vector of dengue, zika, and chikungunya. the analysis involved the quantification of all the costs required for each of the prevention and control st ...201830517314
sequential infection of aedes aegypti mosquitoes with chikungunya virus and zika virus enhances early zika virus transmission.in urban settings, chikungunya, zika, and dengue viruses are transmitted by aedes aegypti mosquitoes. since these viruses co-circulate in several regions, coinfection in humans and vectors may occur, and human coinfections have been frequently reported. yet, little is known about the molecular aspects of virus interactions within hosts and how they contribute to arbovirus transmission dynamics. we have previously shown that aedes aegypti exposed to chikungunya and zika viruses in the same blood ...201830513725
a scoping review of published literature on chikungunya virus.chikungunya virus (chikv) has caused several major epidemics globally over the last two decades and is quickly expanding into new areas. although this mosquito-borne disease is self-limiting and is not associated with high mortality, it can lead to severe, chronic and disabling arthritis, thereby posing a heavy burden to healthcare systems. the two main vectors for chikv are aedes aegypti and aedes albopictus (asian tiger mosquito); however, many other mosquito species have been described as com ...201830496207
mosquitoes modulate leg dynamics at takeoff to accommodate surface roughness.insects perform takeoffs from a nearly unquantifiable number of surface permutations and many use their legs to initiate upward movement prior to the onset of wingbeats, including the mosquito. in this study we examine the unprovoked pre-takeoff mechanics of aedes aegypti mosquitoes from two surfaces of contrasting roughness, one with roughness similar to polished glass and the other comparable to the human forearm. using high-speed videography, we find mosquitos exhibit two distinct leg actions ...201830479315
a novel highly divergent strain of cell fusing agent virus (cfav) in mosquitoes from the brazilian amazon region.classical insect-specific flaviviruses (cisfs) have been widely detected in different countries in the last decades. here, we characterize the near full-length genomes of two cisfs detected in mosquitoes collected in the city of macapá, state of amapá, amazon region of brazil. a total of 105 pools of female mosquitos were analyzed by next-generation sequencing (ngs). comparative genomics and phylogenetic analysis identified three strains of cell fusing agent virus (cfav) and two of culex flavivi ...201830477235
void entry by aedes aegypti (diptera: culicidae) mosquitoes is lower than would be expected by a randomized search.insects enter every passible space on the planet. despite our best efforts, flying insects infiltrate slightly open windows in domiciles, automobiles, storage spaces, and more. is this ubiquitous experience a consequence of insect abundance and probability, or are flying insects adept at detecting passageways? there remains a lack of understanding of insect effectiveness in finding passage through the voids and imperfections in physical barriers in response to attractants, a topic particularly c ...201830476211
global spatial assessment of aedes aegypti and culex quinquefasciatus: a scenario of zika virus exposure.zika virus (zikv) is an arbovirus transmitted mainly by aedes aegypti mosquitoes. recent scientific evidence on culex quinquefasciatus has suggested its potential as a vector for zikv, which may change the current risk zones. we aimed to quantify the world population potentially exposed to zikv in a spatially explicit way, considering the primary vector (a. aegypti) and the potential vector (c. quinquefasciatus). our model combined species distribution modelling of mosquito species with spatiall ...201830474578
alternative insecticides for larval control of the dengue vector aedes aegypti in lao pdr: insecticide resistance and semi-field trial study.the mosquito aedes aegypti is the primary vector of several arboviruses, such as dengue, chikungunya and zika, and represents a major public health problem in southeast asia. in laos, where dengue is reemerging, several ae. aegypti populations from the capital vientiane have shown resistance to the organophosphate temephos, a commonly-used larvicide for public health interventions.201830509299
zikv demonstrates minimal pathologic effects and mosquito infectivity in viremic cynomolgus macaques.to evaluate the effects of zikv infection on non-human primates (nhps), as well as to investigate whether these nhps develop sufficient viremia to infect the major urban vector mosquito, aedes aegypti, four cynomolgus macaques (macaca fascicularis) were subcutaneously infected with 5.0 log10 focus-forming units (ffu) of dna clone-derived zikv strain fss13025 (asian lineage, cambodia, 2010). following infection, the animals were sampled (blood, urine, tears, and saliva), underwent daily health mo ...201830469417
vertical transmission of naturally occurring bunyamwera and insect-specific flavivirus infections in mosquitoes from islands and mainland shores of lakes victoria and baringo in kenya.many arboviruses transmitted by mosquitoes have been implicated as causative agents of both human and animal illnesses in east africa. although epidemics of arboviral emerging infectious diseases have risen in frequency in recent years, the extent to which mosquitoes maintain pathogens in circulation during inter-epidemic periods is still poorly understood. this study aimed to investigate whether arboviruses may be maintained by vertical transmission via immature life stages of different mosquit ...201830452443
cyp-mediated permethrin resistance in aedes aegypti and evidence for trans-regulation.aedes aegypti poses a serious risk to human health due to its wide global distribution, high vector competence for several arboviruses, frequent human biting, and ability to thrive in urban environments. pyrethroid insecticides remain the primary means of controlling adult a. aegypti populations during disease outbreaks. as a result of decades of use, pyrethroid resistance is a global problem. cytochrome p450 monooxygenase (cyp)-mediated detoxification is one of the primary mechanisms of pyrethr ...201830452436
air versus water temperature of aquatic habitats in delhi: implications for transmission dynamics of aedes aegypti.the present study was planned to characterize the microclimate experienced by aedes larvae in different breeding habitats by determining the temperature variations in water kept in containers during different months under natural conditions. the study was conducted in three municipal zones of delhi. in each site, four types of container material (plastic, cement, iron and ceramic) were chosen for recording the water temperature in the containers. daily air and water temperatures (mean, maximum a ...201830451475
operational satellite-based temporal modelling of aedes population in argentina.aedes aegypti is a vector for chikungunya, dengue and zika viruses in latin america and is therefore a large public health problem for the region. for this reason, several inter-institutional and multidisciplinary efforts have been made to support vector control actions through the use of geospatial technologies. this study presents the development of an operational system for the application of free access to remotely sensed products capable of assessing the oviposition activity of ae. aegypti ...201830451465
innate immune response of primary human keratinocytes to west nile virus infection and its modulation by mosquito saliva.west nile virus (wnv) is a flavivirus involved in many human infections worldwide. this arthropod-borne virus is directly co-inoculated with mosquito saliva through the epidermis and the dermis during blood meal. wnv starts replicating in the skin before migrating to the draining lymph node, leading to widespread viremia and in some cases to neurological symptoms. skin is a complex organ composed of different cell types that together perform essential functions such as pathogen sensing, barrier ...201830450338
pharmacological inhibition of inward rectifier potassium channels induces lethality in larval aedes aegypti.the inward rectifier potassium (kir) channels play key roles in the physiology of mosquitoes and other insects. our group, among others, previously demonstrated that small molecule inhibitors of kir channels are promising lead molecules for developing new insecticides to control adult female mosquitoes. however, the potential use of kir channel inhibitors as larvicidal agents is unknown. here we tested the hypothesis that pharmacological inhibition of kir channels in the larvae of aedes aegypti, ...201830445675
a comprehensive review of the manifestations and pathogenesis of zika virus in neonates and adults.the zika virus (zikv) has been slowly becoming an epidemic in different parts of the world. since its discovery in 1947, there have been numerous outbreaks affecting many different populations. currently, there is an ongoing threat of zikv in latin america and the united states of america. zikv is mainly spread by the aedesaegypti mosquito and causes non-specific symptoms such as fever, myalgia, and generalized weakness. in addition to these symptoms, it has been noted the zikv is capable of cau ...201830443460
competition among aedes aegypti larvae.adult aedes aegypti mosquitoes are important vectors of human disease. the size of the adult female affects her success, fitness, and ability to transmit diseases. the size of the adults is determined during the aquatic larval stage. competition among larvae for food influences the size of the pupa and thus the adult. in these experiments, the food level (mg/larva) and the density (larvae/vial) both affect intraspecific competition, which shows up as the interaction of the two factors. furthermo ...201830439951
improved reference genome of aedes aegypti informs arbovirus vector control.female aedes aegypti mosquitoes infect more than 400 million people each year with dangerous viral pathogens including dengue, yellow fever, zika and chikungunya. progress in understanding the biology of mosquitoes and developing the tools to fight them has been slowed by the lack of a high-quality genome assembly. here we combine diverse technologies to produce the markedly improved, fully re-annotated aaegl5 genome assembly, and demonstrate how it accelerates mosquito science. we anchored phys ...201830429615
restriction of zika virus infection and transmission in aedes aegypti mediated by an insect-specific flavivirus.previous studies demonstrated an insect-specific flavivirus, nhumirim virus (nhuv), can suppress growth of west nile virus (wnv) and decrease transmission rates in nhuv/wnv co-inoculated culex quinquefasciatus. to assess whether nhuv might interfere with transmission of other medically important flaviviruses, the ability of nhuv to suppress viral growth of zika virus (zikv) and dengue-2 virus (denv-2) was assessed in aedes albopictus cells. significant reductions in zikv (100,000-fold) and denv- ...201830429457
recent history of aedes aegypti: vector genomics and epidemiology records.aedes aegypti bears the common name "the yellow fever mosquito," although, today, it is of more concern as the major vector of dengue, chikungunya, and, most recently, zika viruses. in the present article, we review recent work on the population genetics of this mosquito in efforts to reconstruct its recent (approximately 600 years) history and relate these findings to epidemiological records of occurrences of diseases transmitted by this species. the two sources of information are remarkably co ...201830464351
longevity of mass-reared, irradiated and packed male anopheles arabiensis and aedes aegypti under simulated environmental field conditions.to ensure the success of a mosquito control programme that integrates the sterile insect technique (sit), it is highly desirable to release sterile males with a maximal lifespan to increase release effectiveness. understanding sterile male survival under field conditions is thus critical for determining the number of males to be released. our study aimed to investigate the effect of mass rearing, irradiation, chilling, packing and release time on irradiated male mosquito longevity.201830463624
yellow fever outbreak in brazil: the puzzle of rapid viral spread and challenges for immunisation.we discuss the complex eco-social factors involved in the puzzle of the unexpected rapid viral spread in the ongoing brazilian yellow fever (yf) outbreak, which has increased the reurbanisation risk of a disease without urban cases in brazil since 1942. indeed, this rapid spatial viral dissemination to the southeast and south regions, now circulating in the atlantic forest fragments close to peri-urban areas of the main brazilian megalopolises (são paulo and rio de janeiro) has led to an exponen ...201830427974
courtship activity, copulation & insemination success in a mosquito vector fed a herbal aphrodisiac: implications for sterile insect technology.in sterile insect technology (sit), mating competitiveness is a pre-condition for the reduction of target pest populations and a crucial parameter for judging efficacy. still, current sit trials are being hindered by decreased effectiveness due to reduced sexual performance of released males. here, we explored the possible role of a herbal aphrodisiac in boosting the mating activity of aedes aegypti.201830425225
conserved motifs in the hypervariable domain of chikungunya virus nsp3 required for transmission by aedes aegypti mosquitoes.chikungunya virus (chikv) is a re-emerging arthropod-borne (arbo)virus that causes chikungunya fever in humans and is predominantly transmitted by aedes aegypti mosquitoes. the chikv replication machinery consists of four non-structural proteins (nsp1-4) that additionally require the presence of a number of host proteins for replication of the viral rna. nsp3 is essential for chikv replication and has a conserved macro, central and c-terminal hypervariable domain (hvd). the hvd is intrinsically ...201830412583
behavioral responses of gravid culex quinquefasciatus, aedes aegypti, and anopheles quadrimaculatus mosquitoes to aquatic macrophyte volatiles.mosquitoes use many cues to assess whether a habitat is conducive for reproduction, possibly including the presence of stimuli from aquatic macrophytes. the effect of water infusions of water hyacinth (eichhornia crassipes), water lettuce (pista stratioles), parrotfeather (myriophyllum aquaticum), and water pennywort (hydrocotyle umbellata) on mosquito oviposition and attraction was investigated. gravid culex quinquefasciatus deposited significantly more egg rafts in water hyacinth, water lettuc ...201830408300
effects of the environmental temperature on aedes aegypti and aedes albopictus mosquitoes: a review.the temperature of the environment is one of the most important abiotic factors affecting the life of insects. as poikilotherms, their body temperature is not constant, and they rely on various strategies to minimize the risk of thermal stress. they have been thus able to colonize a large spectrum of habitats. mosquitoes, such as ae. aegypti and ae. albopictus, vector many pathogens, including dengue, chikungunya, and zika viruses. the spread of these diseases has become a major global health co ...201830404142
anopheles mosquitoes may drive invasion and transmission of mayaro virus across geographically diverse regions.the togavirus (alphavirus) mayaro virus (mayv) was initially described in 1954 from mayaro county (trinidad) and has been responsible for outbreaks in south america and the caribbean. imported mayv cases are on the rise, leading to invasion concerns similar to chikungunya and zika viruses. little is known about the range of mosquito species that are competent mayv vectors. we tested vector competence of 2 mayv genotypes in laboratory strains of six mosquito species (aedes aegypti, anopheles free ...201830403665
insecticide resistance and fitness: the case of four aedes aegypti populations from different brazilian regions.chemical control is still a major strategy to constrain vector density and mitigate pathogen transmission. however, insecticide overuse poses a high selective pressure, favouring the spread of resistance alleles in natural populations. in an insecticide-free environment, a fitness cost is expected in resistant insects when compared to susceptible counterparts. this study investigates whether insecticide resistance to an organophosphate (temephos) and a pyrethroid (deltamethrin) is associated wit ...201830402487
inspiratory muscle training with isokinetic device to help ventilatory weaning in a patient with guillain-barré syndrome by zika virus.zika is an arbovirus infection mainly transmitted by the mosquito aedes aegypti. in 2016, the burden of zika epidemic in brazil was significant. patients affected by zika virus can develop guillain-barré syndrome, evolving to muscle respiratory failure requiring mechanical ventilation. in this setting, delayed recovery of the muscle weakness can result in prolonged weaning, a condition that by itself is related to a high mortality rate. the study is reporting a case of a patient with zika and gu ...201830402297
chemical composition and larvicidal activities of azolla pinnata extracts against aedes (diptera:culicidae).the resistance problem of dengue vectors to different classes of insecticides that are used for public health has raised concerns about vector control programmes. hence, the discovery of alternative compounds that would enhance existing tools is important for overcoming the resistance problem of using insecticides in vectors and ensuring a chemical-free environment. the larvicidal effects of azolla pinnata extracts by using two different extraction methods with methanol solvent against aedes in ...201830399167
isolation and identification of mosquito biting deterrents from the north american mosquito repelling folk remedy plant, matricaria discoidea dc.matricaria discoidea dc. (asteraceae) has documented use as an insect repellent by blackfoot indians and other indigenous groups of north america. this investigation was conducted to evaluate this practice and systematically identify chemical constituents responsible for any insect repelling effect by utilizing a mosquito (aedes aegypti (linnaeus)) biting deterrent bioactivity-directed purification approach. hydrodistilled oil from dried aerial parts of m. discoidea was the most bioactive crude ...201830379955
control of dengue virus in the midgut of aedes aegypti by ectopic expression of the dsrna-binding protein loqs2.dengue virus (denv) is an arbovirus transmitted to humans by aedes mosquitoes1. in the insect vector, the small interfering rna (sirna) pathway is an important antiviral mechanism against denv2-5. however, it remains unclear when and where the sirna pathway acts during the virus cycle. here, we show that the sirna pathway fails to efficiently silence denv in the midgut of aedes aegypti although it is essential to restrict systemic replication. accumulation of denv-derived sirnas in the midgut re ...201830374169
new sulphated flavonoids and larvicidal activity of helicteres velutina k. schum (sterculiaceae).helicteres velutina k. schum (sterculiaceae), commonly known in brazil as 'pitó', is traditionally used by indigenous peoples as insecticides and repellents. the present work reports on the the phytoconstituents from aerial parts of h. velutina and evaluation of the larvicidal potential of its extract. the compounds were isolated using chromatographic techniques and identified by nmr, ir and lc-hrms. this study led to the isolation of a fatty acid, one aliphatic alcohol, four chlorophyll derivat ...201830373225
wing morphometric variability in aedes aegypti (diptera: culicidae) from different urban built environments.aedes aegypti is the main vector of the dengue, zika and several other arboviruses. it is highly adapted to urbanized environments and can be found worldwide. mosquito population control is considered the best strategy for fighting mosquito-borne diseases, making an understanding of their population dynamics vital for the development of more effective vector control programs. this study therefore sought to investigate how different levels of urbanization affect aedes aegypti populations and modu ...201830367678
generation of axenic aedes aegypti demonstrate live bacteria are not required for mosquito development.the mosquito gut microbiome plays an important role in mosquito development and fitness, providing a promising avenue for novel mosquito control strategies. here we present a method for rearing axenic (bacteria free) aedes aegypti mosquitoes, consisting of feeding sterilized larvae on agar plugs containing a high concentration of liver and yeast extract. this approach allows for the complete development to adulthood while maintaining sterility; however, axenic mosquito's exhibit delayed developm ...201830367055
odor-specific daily rhythms in the olfactory sensitivity and behavior of aedes aegypti mosquitoes.many biological processes and behaviors in mosquitoes display rhythmic patterns, allowing for fine tuning to cyclic environmental conditions. in mosquitoes, vector-host interactions are primarily mediated by olfactory signals. previous studies have established that, in the malaria vector anopheles gambiae, rhythmic expression of odorant binding proteins and takeout proteins in the antenna resulted in a corresponding rhythm in olfactory sensitivity to relevant host odors. however, it remained unc ...201830360532
salivary gland extract from aedes aegypti improves survival in murine polymicrobial sepsis through oxidative mechanisms.sepsis is a systemic disease with life-threatening potential and is characterized by a dysregulated immune response from the host to an infection. the organic dysfunction in sepsis is associated with the production of inflammatory cascades and oxidative stress. previous studies showed that aedes aegypti saliva has anti-inflammatory, immunomodulatory, and antioxidant properties. considering inflammation and the role of oxidative stress in sepsis, we investigated the effect of pretreatment with sa ...201830360497
the spread of mosquito-borne viruses in modern times: a spatio-temporal analysis of dengue and chikungunya.since the 1970s, mosquito-borne pathogens have spread to previously disease-free areas, as well as causing increased illness in endemic areas. in particular, dengue and chikungunya viruses, transmitted primarily by aedes aegypti and secondarily by aedes albopictus mosquitoes, represent a threat for up to a third of the world population, and are a growing public health concern. in this study, we assess the spatial and temporal factors related to the occurrences of historic dengue and chikungunya ...201830390927
improvement of mosquito identification by maldi-tof ms biotyping using protein signatures from two body parts.matrix-assisted laser desorption/ionization time-of-flight mass spectrometry technology (maldi-tof ms) is an innovative tool that has been shown to be effective for the identification of numerous arthropod groups including mosquitoes. a critical step in the implementation of maldi-tof ms identification is the creation of spectra databases (db) for the species of interest. mosquito legs were the body part most frequently used to create identification db. however, legs are one of the most fragile ...201830390691
toxic activity, molecular modeling and docking simulations of bacillus thuringiensis cry11 toxin variants obtained via dna shuffling.the cry11 family belongs to a large group of δ-endotoxins that share three distinct structural domains. among the dipteran-active toxins referred to as three-domain cry11 toxins, the cry11aa protein from bacillus thuringiensis subsp. israelensis (bti) has been the most extensively studied. despite the potential of bti as an effective biological control agent, the understanding of cry11 toxins remains incomplete. in this study, five cry11 variants obtained via dna shuffling displayed toxic activi ...201830386315
toward implementation of mosquito sterile insect technique: the effect of storage conditions on survival of male aedes aegypti mosquitoes (diptera: culicidae) during transport.sterile insect technique (sit) is a promising, environmentally friendly alternative to the use of pesticides for insect pest control. however, implementing sit with aedes aegypti (linnaeus) mosquitoes presents unique challenges. for example, during transport from the rearing facility to the release site and during the actual release in the field, damage to male mosquitoes should be minimized to preserve their reproductive competitiveness. the short flight range of male ae. aegypti requires elabo ...201830383264
quantification of permethrin resistance and kdr alleles in florida strains of aedes aegypti (l.) and aedes albopictus (skuse).recent outbreaks of locally transmitted dengue and zika viruses in florida have placed more emphasis on integrated vector management plans for aedes aegypti (l.) and aedes albopictus skuse. adulticiding, primarily with pyrethroids, is often employed for the immediate control of potentially arbovirus-infected mosquitoes during outbreak situations. while pyrethroid resistance is common in ae. aegypti worldwide and testing is recommended by cdc and who, resistance to this class of products has not ...201830356237
an unusual occurrence of stromal keratitis in dengue fever.dengue is a mosquito-borne infection endemic in the tropical and subtropical regions of the world. classic dengue fever is a self-limiting, influenza-like illness transmitted by aedes aegypti mosquito. ophthalmic manifestations though rare can involve both the anterior and posterior segments and are usually associated with the thrombocytopenic state. however, ophthalmic complications such as anterior uveitis and vasculitis suggest immune-mediated pathogenesis. herein, we report a rare case of st ...201830355887
validation of serum aminotransferases levels to define severe dengue fever in children.we aimed to study the pattern of liver-injury in children with dengue fever (df) and validate serum aminotransferase ≥1,000 iu/l as a marker of severe df.201830345242
the sequence of a male-specific genome region containing the sex determination switch in aedes aegypti.aedes aegypti is the principal vector of several important arboviruses. among the methods of vector control to limit transmission of disease are genetic strategies that involve the release of sterile or genetically modified non-biting males, which has generated interest in manipulating mosquito sex ratios. sex determination in ae. aegypti is controlled by a non-recombining y chromosome-like region called the m locus, yet characterisation of this locus has been thwarted by the repetitive nature o ...201830342535
ultrastructural analysis of chikungunya virus dissemination from the midgut of the yellow fever mosquito, aedes aegypti.the transmission cycle of chikungunya virus (chikv) requires that mosquito vectors get persistently infected with the virus, following its oral acqsuisition from a vertebrate host. the mosquito midgut is the initial organ that gets infected with orally acquired chikv. following its replication in the midgut epithelium, the virus exits the midgut and infects secondary tissues including the salivary glands before being transmitted to another host. here, we investigate the pattern of chikv dissemin ...201830340365
the transcriptional response of aedes aegypti with variable extrinsic incubation periods for dengue virus.dengue fever is the most prevalent arboviral disease globally. dengue virus is transmitted primarily by the aedes aegypti mosquito. one measure of the mosquito's efficiency as a vector is the extrinsic incubation period (eip), which is the time between the ingestion of viremic blood and the emergence of virions in the saliva. the longer it takes virus to infect the midgut and traverse to the saliva, the fewer opportunities the mosquito will have to transmit the pathogen over its lifetime. we hav ...201830335126
effectiveness of a field trap barrier system for controlling aedes albopictus: a "removal trapping" strategy.aedes aegypti and aedes albopictus are the main vectors for the transmission of several viral pathogens, in particular, dengue, zika and chikungunya. in the absence of vaccines and treatment, control of aedes mosquitoes is the only means of keeping these diseases in check. aedes control is difficult, and it is, therefore, necessary to evaluate the efficacy of novel control methods, particularly those targeting adult and exophilic ae. albopictus populations.201829463300
host plant forensics and olfactory-based detection in afro-tropical mosquito disease vectors.the global spread of vector-borne diseases remains a worrying public health threat, raising the need for development of new combat strategies for vector control. knowledge of vector ecology can be exploited in this regard, including plant feeding; a critical resource that mosquitoes of both sexes rely on for survival and other metabolic processes. however, the identity of plant species mosquitoes feed on in nature remains largely unknown. by testing the hypothesis about selectivity in plant feed ...201829462150
increased adult aedes aegypti and culex quinquefasciatus (diptera: culicidae) abundance in a dengue transmission hotspot, compared to a coldspot, within kaohsiung city, taiwan.the assumption that vector abundance differences might drive spatial and temporal heterogeneities in vector-borne disease transmission is common, though data supporting it is scarce. here, we present data from two common mosquito species aedes aegypti (linnaeus) and culex quinquefasciatus say, biweekly sampled as adults, from march 2016 through december 2017, with bg-sentinel traps in two neighboring districts of kaohsiung city (kc), taiwan. one district has historically been a dengue transmissi ...201830104501
impact of mosquito age and insecticide exposure on susceptibility of aedes albopictus (diptera: culicidae) to infection with zika virus.zika virus (zikv) is primarily transmitted to humans by aedes aegypti and ae. albopictus. vector⁻virus interactions influencing vector competence vary and depend on biological and environmental factors. a mosquito's chronological age may impact its immune response against virus infection. insecticides, source reduction, and/or public education are currently the best defense against mosquitoes that transmit zikv. this study assessed the effects of a mosquito's chronological age at time of infecti ...201830103526
variation in wolbachia effects on aedes mosquitoes as a determinant of invasiveness and vectorial capacity.wolbachia has been introduced into aedes aegypti mosquitoes to control the spread of arboviruses, such as dengue, chikungunya and zika. studies showed that certain wolbachia strains (such as wmel) reduce replication of dengue viruses in the laboratory, prompting the release of mosquitoes carrying the bacterium into the field, where vectorial capacity can be realistically assessed in relation to native non-carriers. here we apply a new analysis to two published datasets, and show that wmel increa ...201829662096
numerical modeling of mosquito population dynamics of aedes aegypti.the global incidences of dengue virus have increased the interest in studying and understanding the mosquito population dynamics. it is predominantly spread by aedes aegypti in the tropical and sub-tropical countries in the world. understanding these dynamics is important for public health in countries where climatic and environmental conditions are favorable for the propagation of these diseases. for this reason, a new model has been proposed to investigate the population dynamics of mosquitoes ...201829661212
factors related to aedes aegypti (diptera: culicidae) populations and temperature determine differences on life-history traits with regional implications in disease transmission.aedes aegypti (l.) (diptera: culicidae) is a vector of many medically significant viruses in the americas, including dengue virus, chikungunya virus, and zika virus. traits such as longevity, fecundity, and feeding behavior contribute to the ability of ae. aegypti to serve as a vector of these pathogens. both local environmental factors and population genetics could contribute to variability in these traits. we performed a comparative study of ae. aegypti populations from four geographically and ...201829659944
detection of aedes (stegomyia) aegypti (diptera: culicidae) populations in southern alabama following a 26-yr absence and public perceptions of the threat of zika virus.with the establishment of zika virus in the americas, an accurate understanding of the geographic range of its primary vector, aedes (stegomyia) aegypti (l.) (diptera: culicidae), is vital to assessing transmission risk. in an article published in june 2016, hahn and colleagues compiled county-level records in the united states for the presence of ae. aegypti and aedes (stegomyia) albopictus (skuse) (diptera: culicidae) reported between january 1995 and march 2016. despite ecological suitability ...201829659928
coinfection with zika virus (zikv) and dengue virus results in preferential zikv transmission by vector bite to vertebrate host.several tropical cities are permissive to aedes aegypti and dengue virus (denv) endemicity and have allowed for invasion and circulation of zika virus (zikv) in the same areas. people living in arbovirus-endemic regions have been simultaneously infected with ≥2 arboviruses.201829659904
co-breeding association of aedes albopictus (skuse) and aedes aegypti (linnaeus) (diptera: culicidae) in relation to location and container size.the occurrence of major outbreaks of dengue, and other vector borne diseases such as chikungunya and zika in tropical and subtropical regions has rendered control of the diseases a top-priority for many affected countries including malaysia. control of the mosquito vectors aedes aegypti and aedes albopictus through the reduction of breeding sites and the application of insecticides to kill immature forms and adults are the main control efforts to combat these diseases. the present study describe ...201829644025
evaluation of a simple polytetrafluoroethylene (ptfe)-based membrane for blood-feeding of malaria and dengue fever vectors in the laboratory.controlled blood-feeding is essential for maintaining laboratory colonies of disease-transmitting mosquitoes and investigating pathogen transmission. we evaluated a low-cost artificial feeding (af) method, as an alternative to direct human feeding (dhf), commonly used in mosquito laboratories.201829642937
yellow fever.the yellow fever (yf) virus is a flavivirus, transmitted by haemagogus, sabethes or aedes aegypti mosquitoes. the disease is endemic in forest areas in africa and latin america leading to epizootics in monkeys that constitute the reservoir of the disease. there are two forms of yf: sylvatic, transmitted accidentally when approaching the forests, and urban, which can be perpetuated by aedes aegypti. in brazil, the last case of urban yf occurred in 1942. since then, there has been an expansion of ...201829641667
identification of entomopathogenic nematodes and symbiotic bacteria from nam nao national park in thailand and larvicidal activity of symbiotic bacteria against aedes aegypti and aedes albopictus.entomopathogenic nematodes (epns) that are symbiotically associated with xenorhabdus and photorhabdus bacteria can kill target insects via direct infection and toxin action. there are limited reports identifying such organisms in the national park of thailand. therefore, the objectives of this study were to identify epns and symbiotic bacteria from nam nao national park, phetchabun province, thailand and to evaluate the larvicidal activity of bacteria against aedes aegypti and ae. albopictus. a ...201829641570
suppression of the pelo protein by wolbachia and its effect on dengue virus in aedes aegypti.the endosymbiont wolbachia is known to block replication of several important arboviruses, including dengue virus (denv), in the mosquito vector aedes aegypti. so far, the exact mechanism of this viral inhibition is not fully understood. a recent study in drosophila melanogaster has demonstrated an interaction between the pelo gene and drosophila c virus. in this study, we explored the possible involvement of the pelo protein, that is involved in protein translation, in wolbachia-mediated antivi ...201829641562
exploring mosquito fauna of majuro atoll (republic of marshall islands) in the context of zika outbreak.first autochthonous zika clinical case was reported in the republic of marshall islands (rmi) on majuro atoll in february 2016. an entomological survey of mosquito larvae and adult populations was carried out in four areas of majuro, the most populated atoll of rmi encompassing different habitats (forest, rural, or urban) including some with confirmed clinical zika cases to evaluate which mosquito species could be involved in the zika transmission. a total of 2,367 immature and adult mosquito sp ...201829635382
a sex-structured model with birth pulse and release strategy for the spread of wolbachia in mosquito population.dengue fever is one of the most important diseases causing illness and death all over the world, which brings tremendous threat to peoples' life and property security, especially in the undeveloped areas. the main vector, aedes aegypti, must be controlled to prevent the transmission of dengue. there are a variety of methods to control it. wolbachia is an innovative bacterium which breaks the dengue transmission cycle for its characteristics of cytoplasmic incompatibility and maternal transmissio ...201829625205
efficacy of aedes aegypti control by indoor ultra low volume (ulv) insecticide spraying in iquitos, peru.aedes aegypti is a primary vector of dengue, chikungunya, zika, and urban yellow fever viruses. indoor, ultra low volume (ulv) space spraying with pyrethroid insecticides is the main approach used for ae. aegypti emergency control in many countries. given the widespread use of this method, the lack of large-scale experiments or detailed evaluations of municipal spray programs is problematic.201829624581
erratum to "comparative oviposition site selection in containers by aedes aegypti and aedes albopictus (diptera: culicidae) from florida". 201829618025
a comparative analysis of corpora allata-corpora cardiaca microrna repertoires revealed significant changes during mosquito metamorphosis.the corpora allata (ca) are a pair of endocrine glands with neural connections to the brain and close association with another neuroendocrine organ, the corpora cardiaca (cc). the ca from adult female aedes aegypti mosquitoes synthesize fluctuating levels of juvenile hormone (jh), which have been linked to the ovarian development and are influenced by nutritional signals. in this study, we investigated the potential involvement of micrornas (mirnas), a type of small non-coding rnas, in the regul ...201829605533
mosquito responses to trait- and density-mediated interactions of predation.mosquito and predatory larvae often share the same habitat. predators may influence mosquito prey populations through both lethal effect and non-lethal pathways. a series of experimental manipulations were used to distinguish between lethal (density-mediated interaction) and non-lethal (trait-mediated interaction) effects in a model system comprised of invasive prey mosquito, aedes aegypti, and a predatory mosquito toxorhynchites rutilus. treatments with predators present or manipulations mimick ...201829594613
effect of relaxation of deltamethrin pressure on metabolic resistance in a pyrethroid-resistant aedes aegypti (diptera: culicidae) strain harboring fixed p989p and g1016g kdr alleles.mutation of the voltage-gated sodium channel genes or knockdown resistance (kdr) and metabolic resistance in aedes aegypti (l.) (diptera: culicidae) are important resistance mechanisms against pyrethroids. the present study investigated the effect of relaxation of deltamethrin selection pressure on the level of mixed-function oxidases (mfo), when the allele frequency of s989p+v1016g mutations is fixed in a resistant ae. aegypti strain (upk-r) from chiang mai, thailand. the mosquitoes were divide ...201829590378
maternal invasion history of aedes aegypti and aedes albopictus into the isthmus of panama: implications for the control of emergent viral disease agents.despite an increase in dengue outbreaks and the arrival of chikungunya and zika disease in panama, studies on the demographic history of the invasive aedes mosquitoes that are the principle vectors of these diseases are still lacking in this region. here, we assess the genetic diversity of these mosquitoes in order to decipher their invasion histories into the isthmus of panama. dna sequences from the mitochondrial cytochrome c oxidase i gene obtained from 30 localities in 10 provinces confirmed ...201829579112
frequency and intensity of pyrethroid resistance through the cdc bottle bioassay and their association with the frequency of kdr mutations in aedes aegypti (diptera: culicidae) from mexico.the control of aedes aegypti (l.), the main urban vector that causes arboviral diseases such as dengue, chikungunya and zika, has proved to be a challenge because of a rapid increase in insecticide resistance. therefore, adequate monitoring of insecticide resistance is an essential element in the control of ae. aegypti and the diseases it transmits. we estimated the frequency and intensity (resistance frequency rapid diagnostic test [f-rdt] and resistance intensity rapid diagnostic test [i-rdt]) ...201829575404
population dynamics of engineered underdominance and killer-rescue gene drives in the control of disease vectors.a number of different genetics-based vector control methods have been proposed. two approaches currently under development in aedes aegypti mosquitoes are the two-locus engineered underdominance and killer-rescue gene drive systems. each of these is theoretically capable of increasing in frequency within a population, thus spreading associated desirable genetic traits. thus they have gained attention for their potential to aid in the fight against various mosquito-vectored diseases. in the case ...201829570717
repellent activity of trig (n-n diethyl benzamide) against man-biting mosquitoes.a study was conducted to assess efficacy of a new repellent brand trig (15% n-n diethyl benzamide) when compared to deet (20% n-n methyl toluamide). the repellents were tested in laboratory and field. in the laboratory, the repellence was tested on human volunteers, by exposing their repellent-treated arms on starved mosquitoes in cages for 3 minutes at hourly intervals, while counting the landing and probing attempts. anopheles gambiae and aedes aegypti mosquitoes were used. field evaluation wa ...201829552040
hr38, an ortholog of nr4a family nuclear receptors, mediates 20-hydroxyecdysone regulation of carbohydrate metabolism during mosquito reproduction.the aedes aegypti mosquito is the principal vector for many dangerous human viral diseases. carbohydrate metabolism (cm) is essential for supplying the energy necessary for host seeking, blood digestion and rapid egg development of this vector insect. the steroid hormone 20-hydroxyecdysone (20e) and the ecdysone receptor (ecr) are important regulators of cm, coordinating it with female reproductive events. we report here that the nr4a nuclear receptor ahr38 plays a critical role in mediating the ...201829526770
jh biosynthesis and hemolymph titers in adult male aedes aegypti mosquitoes.juvenile hormone (jh) is a major hormonal regulator in insects. in aedes aegypti females, jh signals the completion of the ecdysis to the adult stage and initiates reproductive processes. although the regulation of jh synthesis and titer in ae. aegypti females has been extensively studied, relatively little is known about changes of jh synthesis and titers in male mosquitoes, as well as on the roles of jh controlling male reproductive biology. a better understanding of male mosquito reproductive ...201829526769
mosquito pornoscopy: observation and interruption of aedes aegypti copulation to determine female polyandric event and mixed progeny.ades aegypti is the most important arbovirus vector in the world, and new strategies are under evaluation. biological studies mentioning the occurrence of a second mate in aedes aegypti can interfere with vector control program planning, which involves male mosquito release technique. this study presents different experiments to show the occurrence of mixed progeny. mixed male crosses (using a combination of different type of males in confinement with virgin females) showed no polyandric female. ...201829518112
comparative oviposition site selection in containers by aedes aegypti and aedes albopictus (diptera: culicidae) from florida.invasive container mosquito species aedes aegypti (l.) and ae. albopictus (skuse) frequently co-occur in nature where they are typically segregated spatially by distinctive macrohabitat preferences that may select for different oviposition strategies. in a standard cage environment, we compared oviposition site selection by individual gravid females of these species exposed to variable numbers of water-holding cups and the presence or absence of a rugose wettable container lining. offered plasti ...201829514300
building infestation index for aedes aegypti and occurrence of dengue fever in the municipality of foz do iguaçu, paraná, brazil, from 2001 to 2016.the building infestation index (bii) uses the rapid assay of the larval index for aedes aegypti (liraa) to express the relationship between positive and surveyed properties. we evaluated liraa and the relationship between the bii and climate variables for dengue cases in foz do iguaçu municipality, paraná.201829513847
occurrence of sweet refuse at disposal sites: rainwater retention capacity and potential breeding opportunities for aedes aegypti.nectar is the staple diet of adult mosquitoes in the wild, but its availability is inconsistent and can be affected by rainfall. in urban centers, aedes vectors commonly use man-made containers as their major habitat; however, they can colonize any items replenished by rainfall. garbage output has increased significantly in recent years, at a time when collection frequency is reducing. such garbage usually includes organic components, some of which are sweet and can be fed upon by other animals ...201829512008
the non-canonical notch signaling is essential for the control of fertility in aedes aegypti.the notch signaling pathway is a highly evolutionarily-conserved cell-cell signaling pathway that regulates many events during development. it plays a pivotal role in the regulation of fundamental cellular processes, such as cell proliferation, stem cell maintenance, and differentiation during embryonic and adult development. however, functions of notch signaling in aedes aegypti, the major mosquito vector for dengue, are largely unknown. in this study, we identified a unique feature of a. aegyp ...201829505577
dengue knowledge in indoor dengue patients from low socioeconomic class; aetiology, symptoms, mode of transmission and prevention.dengue fever has emerged as an emerging public health issue during last decade bearing significant morbidity and economic burden particularly in third world countries. current study aims to assess various domains of knowledge of indoor dengue patients..201829504327
resistance of aedes aegypti (diptera: culicidae) populations to deltamethrin, permethrin, and temephos in cambodia.dengue fever is a major public health concern, including 185,000 annual cases in cambodia. aedes aegypti is the primary vector for dengue transmission and is targeted with insecticide treatments. this study characterized the insecticide resistance status of ae aegypti from rural and urban locations. the susceptibility to temephos, permethrin, and deltamethrin of ae aegypti was evaluated in accordance with world health organization instructions. all the field populations showed lower mortality ra ...201829502428
bacterial microbiota of aedes aegypti mosquito larvae is altered by intoxication with bacillus thuringiensis israelensis.insect microbiota is a dynamic microbial community that can actively participate in defense against pathogens. bacillus thuringiensis (bt) is a natural entomopathogen widely used as a bioinsecticide for pest control. although bt's mode of action has been extensively studied, whether the presence of microbiota is mandatory for bt to effectively kill the insect is still under debate. an association between a higher tolerance and a modified microbiota was already evidenced but a critical point rema ...201829499735
anti-diuretic action of a capa neuropeptide against a subset of diuretic hormones in the disease vector aedes aegypti.the mosquito aedes aegypti is a vector responsible for transmitting various pathogens to humans, and their prominence as chief vectors of human disease is largely due to their anthropophilic blood feeding behaviour. larval stage mosquitoes must deal with the potential dilution of their haemolymph in freshwater, whereas the haematophagus a. aegypti female faces the challenge of excess ion and water intake after a blood meal. the excretory system, composed of the malpighian tubules (mts) and hindg ...201829496779
dengue in araraquara, state of são paulo: epidemiology, climate and aedes aegypti infestation.objective to describe the epidemiology of dengue in a medium-sized city in the state of são paulo. methods data, such as circulating serotypes, severe cases and deaths, age group, sex, among others, were obtained on reported and confirmed dengue cases in araraquara, state of são paulo, between 1991 and 2015. climatic and infestation data were also analyzed. these variables were evaluated descriptively, using statistical measures such as frequencies, averages, minimum and maximum. dengue incidenc ...201829489994
truck-mounted natular 2ec (spinosad) ulv residual treatment in a simulated urban environment to control aedes aegypti and aedes albopictus in north florida.preemptive treatment of dry habitats with an ultra-low volume (ulv) residual larvicide may be effective in an integrated vector management program to control populations of container-inhabiting aedes mosquitoes, key vectors of zika, dengue, and chikungunya viruses. we exposed dry, artificial containers placed in exposed and protected locations to natular 2ec (spinosad) larvicide applied with a truck-mounted ulv sprayer in a simulated urban setting in north florida, and later introduced water and ...201831442121
ultra-low volume application of spinosad (natular 2ec) larvicide as a residual in a tropical environment against aedes and anopheles species.we investigated the efficacy of a liquid larvicide, natular 2ec® (spinosad), applied with ultra-low volume sprayer as a residual application during the dry season in southeastern thailand against 4 medically important species-aedes aegypti, ae. albopictus, anopheles dirus, and an. minimus. we found that this larvicide could be applied as a residual to dry areas known to collect water and potentially still be effective after rains or irrigation, which could increase the flexibility and efficiency ...201831442120
effect of travel speed on dispersion of aqualuer 20-20 sprayed by a truck-mounted ultra-low-volume sprayer against caged aedes aegypti1.the effect of travel speed of a truck-mounted ultra-low-volume (ulv) sprayer on its application efficacy was studied at st. johns county fairground, elkton, fl, during summer 2015. the efficacy was assessed by spray deposition, droplet size spectrum, and 24-h mortality of caged adult aedes aegypti, using 2 rows of sampling locations, 15 m apart and spread up to 122 m from the spray. each location had a bioassay cage and an impinger droplet sampler, 1 m apart from each other, at 1.5 m off the gro ...201831442115
application efficacy of vectobac wdg against larval aedes aegypti using thermal fog technology.the effectiveness of thermal fog application of bacillus thuringiensis israelensis (vectobac® wdg) against larval aedes aegypti was evaluated in open and forest canopied environments in northeastern florida, using a handheld igeba® tf34 thermal fogger (with water conversion kit). the product was applied at the maximum label rate of 1 kg/ha to linear transects, in each environment, containing empty 0.5-liter cups. after application, late-2nd to early-3rd instars of ae. aegypti were added to each ...201831442110
ultra-low volume application of spinosad (natular 2ec) as a residual in a hot-arid environment against aedes aegypti.the invasive aedes aegypti is an important disease vector increasing in frequency in hot-arid regions of the usa such as the southwest. within hot-arid surroundings this mosquito may be confined to peridomestic locations that tend to be cooler and humid, such as in lush, irrigated ornamental vegetation surrounding homes. however, to reach these habitat refugia, ultra-low volume (ulv) applications of insecticides targeting this mosquito must retain efficacy after being sprayed from the air or str ...201831442108
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