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costs and benefits of mosquito refractoriness to malaria parasites: implications for genetic variability of mosquitoes and genetic control of malaria.the problem of fitness costs associated with host resistance to parasitism is related to the evolution of parasite virulence, population genetic diversity and the dynamics of host-parasite relationships, and proposed strategies for disease control through the genetic manipulation of mosquito vectors. two aedes aegypti populations, refractory and susceptible to plasmodium gallinaceum, were previously selected from the moyo-in-dry strain (moyo) through inbreeding (f = 0.5). reproductive success an ...199728565348
reproductive isolation in stegomyia mosquitoes. ii. hybrid breakdown between aedes aegypti and a. mascarensis. 197028564933
a potent larvicidal agent against aedes aegypti mosquito from cardanol.cardanol is a constituent of cashew nut shell liquid that presents larvicidal activity against aedes aegypti. the isolation of cardanol is somewhat troublesome, however, in this work we describe an efficient and inexpensive method to obtain it as a pure material. the compound was used as starting material to make chemical transformation leading to saturated cardanol, epoxides and, halohydrins. these derivatives were tested for toxicity against aedes aegypti larvae. the results showed that iodohy ...201728562821
ascogregarina taiwanensis infection in aedes aegypti and aedes albopictus in santa catarina, south brazil.this study registers ascogregarina spp. infection in field populations of aedes aegypti and aedes albopictus in a subtropical region of brazil.201728562761
molecular studies with aedes (stegomyia) aegypti (linnaeus, 1762), mosquito transmitting the dengue virus.dengue is an infectious viral disease, which can present a wide clinical picture, ranging from oligo or asymptomatic forms, to bleeding and shock, and can progress to death. the disease problem has increased in recent years, especially in urban and suburban areas of tropical and subtropical regions. there are five dengue viruses, called serotypes (den-1, den-2, den-3, den-4, and den-5), which belong to the flaviviridae family and are transmitted to humans through infected mosquito bites, with th ...201728560571
metofluthrin: investigations into the use of a volatile spatial pyrethroid in a global spread of dengue, chikungunya and zika viruses.metofluthrin reduces biting activity in aedes aegypti through the confusion, knockdown, and subsequent kill of a mosquito. a geographical spread in dengue, chikungunya, and zika viruses, increases intervention demands. response to a zika outbreak may require a different strategy than dengue, as high-risk individuals, specifically pregnant women, need to be targeted.201728558804
local introduction and heterogeneous spatial spread of dengue-suppressing wolbachia through an urban population of aedes aegypti.dengue-suppressing wolbachia strains are promising tools for arbovirus control, particularly as they have the potential to self-spread following local introductions. to test this, we followed the frequency of the transinfected wolbachia strain wmel through ae. aegypti in cairns, australia, following releases at 3 nonisolated locations within the city in early 2013. spatial spread was analysed graphically using interpolation and by fitting a statistical model describing the position and width of ...201728557993
activation of aedes aegypti prophenoloxidase-3 and its role in the immune response against entomopathogenic fungi.serine protease cascade-mediated melanization is an important innate immune response in insects and crustaceans, which involves the proteolytic activation of prophenoloxidase (ppo). in this study, we investigated the role of aedes aegypti ppo3 in antifungal immune defence. we expressed and purified recombinant ppo3 (rppo3) in escherichia coli and demonstrated that rppo3 was activated by ethanol and, to a lesser extent, by cetylpyridinium chloride. in the presence of cu(2+) , rppo3 exhibited enzy ...201728556276
barriers to hybridization between aedes aegypti and aedes albopictus (diptera: culicidae). 196728556103
evolutionary strategies in life table characteristics among feral and urban strains of aedes aegypti (l.). 197228555736
elevation as a proxy for mosquito-borne zika virus transmission in the americas.when zika virus (zikv) first began its spread from brazil to other parts of the americas, national-level travel notices were issued, carrying with them significant economic consequences to affected countries. although regions of some affected countries were likely unsuitable for mosquito-borne transmission of zikv, the absence of high quality, timely surveillance data made it difficult to confidently demarcate infection risk at a sub-national level. in the absence of reliable data on zikv activi ...201728542540
wmel limits zika and chikungunya virus infection in a singapore wolbachia-introgressed ae. aegypti strain, wmel-sg.zika (zikv) and chikungunya (chikv) viruses are emerging aedes-borne viruses that are spreading outside their known geographic range and causing wide-scale epidemics. it has been reported that these viruses can be transmitted efficiently by ae. aegypti. recent studies have shown that ae. aegypti when transinfected with certain wolbachia strains shows a reduced replication and dissemination of dengue (denv), chikungunya (chikv), and yellow fever (yfv) viruses. the aim of this study was to determi ...201728542240
a new in vitro bioassay system for the discovery and quantitative evaluation of mosquito repellents.mosquitoes vector many pathogens that cause human diseases. repellents play a significant role in reducing the risk of these diseases by preventing mosquito bites. in this paper, we are reporting an ali and khan (a & k), large cage in vitro bioassay system that can effectively be used to measure repellency of compounds against mosquitoes. the system uses temperature as a landing and feeding stimulus, and collagen as a feeding substrate. the minimum effective dose (med) of deet (n, n-diethyl-meta ...201728541424
uncovering the repertoire of endogenous flaviviral elements in aedes mosquito genomes.endogenous viral elements derived from nonretroviral rna viruses have been described in various animal genomes. whether they have a biological function, such as host immune protection against related viruses, is a field of intense study. here, we investigated the repertoire of endogenous flaviviral elements (efves) in aedes mosquitoes, the vectors of arboviruses such as dengue and chikungunya viruses. previous studies identified three efves from aedes albopictus cell lines and one from aedes aeg ...201728539440
genomic epidemiology reveals multiple introductions of zika virus into the united states.zika virus (zikv) is causing an unprecedented epidemic linked to severe congenital abnormalities. in july 2016, mosquito-borne zikv transmission was reported in the continental united states; since then, hundreds of locally acquired infections have been reported in florida. to gain insights into the timing, source, and likely route(s) of zikv introduction, we tracked the virus from its first detection in florida by sequencing zikv genomes from infected patients and aedes aegypti mosquitoes. we s ...201728538723
enhanced larvicidal, antibacterial, and photocatalytic efficacy of tio2 nanohybrids green synthesized using the aqueous leaf extract of parthenium hysterophorus.titanium dioxide nanoparticles are emerging as a biocompatible nanomaterial with multipurpose bioactivities. in this study, titanium dioxide (tio2) nanoparticles were effectively synthesized using the aqueous leaf extracts of parthenium hysterophorus prepared by microwave irradiation. tio2 nanoparticles were fabricated by treating the p. hysterophorus leaf extracts with the tio4 solution. biologically active compounds such as alcohols, phenols, alkanes, and fluoroalkanes were involved in bioredu ...201728537028
rna-seq comparison of larval and adult malpighian tubules of the yellow fever mosquito aedes aegypti reveals life stage-specific changes in renal function.introduction: the life history of aedes aegypti presents diverse challenges to its diuretic system. during the larval and pupal life stages mosquitoes are aquatic. with the emergence of the adult they become terrestrial. this shifts the organism within minutes from an aquatic environment to a terrestrial environment where dehydration has to be avoided. in addition, female mosquitoes take large blood meals, which present an entirely new set of challenges to salt and water homeostasis. methods: to ...201728536536
de novo generation and characterization of new zika virus isolate using sequence data from a microcephaly case.zika virus (zikv) has recently emerged and is the etiological agent of congenital zika syndrome (czs), a spectrum of congenital abnormalities arising from neural tissue infections in utero. herein, we describe the de novo generation of a new zikv isolate, zikvnatal, using a modified circular polymerase extension reaction protocol and sequence data obtained from a zikv-infected fetus with microcephaly. zikvnatal thus has no laboratory passage history and is unequivocally associated with czs. zikv ...201728529976
outbreak of zika virus infection in singapore: an epidemiological, entomological, virological, and clinical analysis.an outbreak of zika virus infection was detected in singapore in august, 2016. we report the first comprehensive analysis of a national response to an outbreak of zika virus infection in asia.201728527892
 introducción. el virus del zika (zikv) es un flavivirus con envoltura, transmitido a los seres humanos principalmente por el vector aedes aegypti. la infección por zikv se ha asociado con un gran neurotropismo y con efectos neuropáticos, como el síndrome de guillain-barré en el adulto y la microcefalia fetal y posnatal, así como con un síndrome de infección congénita similar al producido por el virus de la rubéola (rv).objetivo. comparar las estructuras moleculares de la proteína de envoltura e ...201728527274
transovarial transmission of denv in aedes aegypti in the amazon basin: a local model of xenomonitoring.transovarial transmission of dengue virus in aedes spp. mosquitoes is considered an important mechanism for the maintenance of the virus in nature and may be implicated in the occurrence of outbreaks and epidemics of the disease. however, there are few studies involving transovarial transmission and viral vector monitoring as a surveillance tool and control strategy. the present study evaluated transovarial transmission of dengue virus in aedes aegypti populations as a xenomonitoring strategy in ...201728526066
zika virus: obstetric and pediatric anesthesia considerations.as of november 2016, the florida department of health (fdh) and the centers for disease control and prevention have confirmed more than 4000 travel-related zika virus (zikv) infections in the united states with >700 of those in florida. there have been 139 cases of locally acquired infection, all occurring in miami, florida. within the us territories (eg, puerto rico, us virgin islands), >30,000 cases of zikv infection have been reported. the projected number of individuals at risk for zikv infe ...201728525510
impact of simultaneous exposure to arboviruses on infection and transmission by aedes aegypti mosquitoes.the recent emergence of both chikungunya and zika viruses in the americas has significantly expanded their distribution and has thus increased the possibility that individuals may become infected by more than one aedes aegypti-borne virus at a time. recent clinical data support an increase in the frequency of coinfection in human patients, raising the likelihood that mosquitoes could be exposed to multiple arboviruses during one feeding episode. the impact of coinfection on the ability of releva ...201728524874
eco-friendly and cost-effective ag nanocrystals fabricated using the leaf extract of habenaria plantaginea: toxicity on six mosquito vectors and four non-target species.recently, the biofabrication of metal nanoparticles has gained wide interest owing to its inherent features such as swift, simplicity, eco-friendliness, and cheaper costs. different green-reducing agents led to the production of nanoparticles with varying toxicity on insects. in the current study, silver nanoparticles (agnps) were successfully synthesized using habenaria plantaginea leaf extract. ag nanoparticles were studied by uv-vis spectroscopy (uv-vis), x-ray diffraction (xrd), fourier tran ...201728523613
quantitative structure-activity relationship study of amide mosquito repellents.a quantitative structure-activity relationship (qsar) study on 43 amide repellents was carried out by the heuristic method in order to reveal the correlations between molecular parameters of these amides and their repellency against aedes aegypti. sketches and optimizations of molecular structures were achieved by the gaussian software package. generation and screening of molecular parameters were accomplished using codessa 2.7.10 software. the leave-one-out method was applied for the model vali ...201728521600
heat in the southeastern united states: characteristics, trends, and potential health impact.high summer temperatures in extratropical areas have an impact on the public's health, mainly through heat stress, high air pollution concentrations, and the transmission of tropical diseases. the purpose of this study is to examine the current characteristics of heat events and future projections of summer apparent temperature (at)-and associated health concerns-throughout the southeastern united states. synoptic climatology was used to assess the atmospheric characteristics of extreme heat day ...201728520817
evolutionary enhancement of zika virus infectivity in aedes aegypti mosquitoes.zika virus (zikv) remained obscure until the recent explosive outbreaks in french polynesia (2013-2014) and south america (2015-2016). phylogenetic studies have shown that zikv has evolved into african and asian lineages. the asian lineage of zikv was responsible for the recent epidemics in the americas. however, the underlying mechanisms through which zikv rapidly and explosively spread from asia to the americas are unclear. non-structural protein 1 (ns1) facilitates flavivirus acquisition by m ...201728514450
a location-specific spreadsheet for estimating zika risk and timing for zika vector surveillance, using us military facilities as an example.local zika virus transmission in the united states involving one or both of the known vector species, aedes aegypti and ae. albopictus, is of major concern. to assist efforts to anticipate the risks of transmission, we developed an excel spreadsheet tool that uses vector and virus temperature thresholds, remotely sensed maximum temperature, and habitat suitability models to answer the questions: "is zika transmission likely here?" and "when should we conduct vector surveillance?" an example spre ...201728511272
laboratory and semi-field evaluations of two (transfluthrin) spatial repellent devices against aedes aegypti (l.) (diptera: culicidae).two transfluthrin-based spatial repellent products (raid dual action insect repellent and home freshener and raid shield (currently not commercially available), sc johnson, racine wi) were evaluated for spatial repellent effects against female aedes aegypti (l.) mosquitoes under laboratory (wind tunnel) and semi-field (outdoor enclosure) conditions. the placement of either product in the wind tunnel significantly reduced host-seeking behaviors. the mean baseline (control) landing counts for the ...201728511270
[arboviruses also have an american dream].some arboviruses that originated in the old world have been introduced by humans into the american continent. the first of them was the yellow fever virus, coming from the west african coast with slaves in the 17th-19th centuries, followed by dengue viruses, which were always prevalent within the americas. next was thewest nile virus, introduced in new york in 1999, that spread in only a few years over the whole continent. then, chikungunya virus arrived on saint martin island in 2013 after its ...201728509947
life-shortening wolbachia infection reduces population growth of aedes aegypti.wolbachia bacteria are being introduced into natural populations of vector mosquitoes, with the goal of reducing the transmission of human diseases such as zika and dengue fever. the successful establishment of wolbachia infection is largely dependent on the effects of wolbachia infection to host fitness, but the effects of wolbachia infection on the individual life-history traits of immature mosquitoes can vary. here, the effects of life-shortening wolbachia (wmelpop) on population growth of in ...201728506794
reemergence of yellow fever in ethiopia after 50 years, 2013: epidemiological and entomological investigations.yellow fever (yf) is a viral hemorrhagic disease transmitted by aedes mosquito species. approximately, 200,000 cases and 30,000 deaths occur worldwide every year. in ethiopia, the last outbreak was reported in 1966 with 2200 cases and 450 deaths. a number of cases with deaths from unknown febrile illness reported from south ari district starting from november 2012. this investigation was conducted to identify the causative agent, source of the outbreak and recommend appropriate interventions.201728506254
the midgut transcriptome of aedes aegypti fed with saline or protein meals containing chikungunya virus reveals genes potentially involved in viral midgut escape.the mosquito aedes aegypti is the primary vector for medically important arthropod-borne viruses, including chikungunya virus (chikv). following oral acquisition, an arbovirus has to persistently infect several organs in the mosquito before becoming transmissible to another vertebrate host. a major obstacle an arbovirus has to overcome during its infection cycle inside the mosquito is the midgut escape barrier, representing the exit mechanism arboviruses utilize when disseminating from the midgu ...201728506207
aedes aegypti (l.) mosquitoes in trinidad, west indies: longevity case studies.few laboratory and field studies have reported long survival periods for ae. aegypti females and even fewer have designed experiments to characterize this important life history trait. this study was conducted under laboratory conditions to determine the number of blood meals taken by individual females, the number of eggs laid per individual female, the length of the gonotrophic cycle, and the duration of female survival. the results showed individual females oviposited between 670 and 1,500 eg ...201728504438
influence of shading and pedestrian traffic on the preference of aedes (stegomyia) aegypti (diptera: culicidae) for oviposition microenvironments.aedes aegypti mosquitoes are highly adaptable to abiotic stimuli. to evaluate the influence of shading and pedestrian traffic on the preference of ae. aegypti for oviposition microenvironments, 20 sites were sampled weekly using ovitraps within the perimeter of universidade federal do espírito santo, located in são mateus, espírito santo, brazil. a spatial and statistical analysis was performed in order to assess the relationship between shading time, pedestrian traffic, and the presence of biol ...201728504433
isolation of an insulin-like peptide from the asian malaria mosquito, anopheles stephensi, that acts as a steroidogenic gonadotropin across diverse mosquito taxa.many insulin-like peptides (ilps) have been identified in insects, yet only a few were isolated in their native form for structural and functional studies. antiserum produced to ilp3 in aedes aegypti was used in a radioimmunoassay to monitor the purification of an ilp from heads of adult an. stephensi and recognized the ilp in other immunoassays. the structure of the purified peptide matched that predicted for the ilp3 in this species. the native form stimulated ecdysteroid production by ovaries ...201728502740
transmission incompetence of culex quinquefasciatus and culex pipiens pipiens from north america for zika virus.abstractin late 2014, zika virus (zikv; flaviviridae, flavivirus) emerged as a significant arboviral disease threat in the western hemisphere. aedes aegypti and aedes albopictus have been considered the principal vectors of zikv in the new world due to viral isolation frequency and vector competence assessments. limited reports of culex transmission potential have highlighted the need for additional vector competence assessments of north american culex species. accordingly, north american culex ...201728500817
fta cards facilitate storage, shipment, and detection of arboviruses in infected aedes aegypti collected in adult mosquito traps.abstractthe utility of applying infected aedes aegypti to flinders technology associates (fta(®)) cards for storage, transport, and detection of dengue, zika, and barmah forest viruses was assessed in laboratory-based experiments. the mosquitoes had been removed from gravid aedes traps maintained under conditions of high temperature and humidity. rna of all viruses could be detected in infected mosquitoes on fta cards either individually or in pools with uninfected mosquitoes, and stored for up ...201728500814
metazoan ribosome inactivating protein encoding genes acquired by horizontal gene transfer.ribosome inactivating proteins (rips) are rna n-glycosidases that depurinate a specific adenine residue in the conserved sarcin/ricin loop of 28s rrna. these enzymes are widely distributed among plants and their presence has also been confirmed in several bacterial species. recently, we reported for the first time in silico evidence of rip encoding genes in metazoans, in two closely related species of insects: aedes aegypti and culex quinquefasciatus. here, we have experimentally confirmed the p ...201728500327
interaction of flavivirus with their mosquito vectors and their impact on the human health in the americas.some of the major arboviruses with public health importance, such as dengue, yellow fever, zika and west nile virus are mosquito-borne or mosquito-transmitted flavivirus. their principal vectors are from the family culicidae, aedes aegypti and aedes albopictus being responsible of the urban cycles of dengue, zika and yellow fever virus. these vectors are highly competent for transmission of many arboviruses. the genetic variability of the vectors, the environment and the viral diversity modulate ...201728499872
potential of a northern population of aedes vexans (diptera: culicidae) to transmit zika virus.zika virus is an emerging arbovirus of humans in the western hemisphere. with its potential spread into new geographical areas, it is important to define the vector competence of native mosquito species. we tested the vector competency of aedes vexans (meigen) from the lake agassiz plain of northwestern minnesota and northeastern north dakota. aedes aegypti (l.) was used as a positive control for comparison. mosquitoes were fed blood containing zika virus and 2 wk later were tested for viral inf ...201728499036
larval interference competition between the native neotropical mosquito limatus durhamii and the invasive aedes aegypti improves the fitness of both species.interspecific competition with native species during biological invasions can sometimes limit alien expansion. we aimed to determine the potential ecological effects of limatus durhamii theobald 1901, a native neotropical mosquito (diptera: culicidae) species, on the invasive species aedes (stegomyia) aegypti (linnaeus 1762) that breeds in the same artificial water containers. development time and adult dry mass were measured in 3 rearing conditions: control (a single larva), intraspecific compe ...201728497885
zingiber cernuum (zingiberaceae) essential oil as effective larvicide and oviposition deterrent on six mosquito vectors, with little non-target toxicity on four aquatic mosquito predators.mosquitoes are responsible for the transmission of many pathogens and parasites, which cause serious diseases in humans and animals. currently, botanical products have been suggested as alternative tools in the fight against arthropod vectors. in this study, the essential oil (eo) extracted from zingiber cernuum was tested as larvicide and oviposition deterrent on six mosquito species of public health relevance, including malaria and zika virus vectors. the eo showed high toxicity on third insta ...201728497331
development of nanoemulsion from vitex negundo l. essential oil and their efficacy of antioxidant, antimicrobial and larvicidal activities (aedes aegypti l.).it is believed that nanoemulsions were emerged as a promising candidate to improve the qualities of natural essential oil towards antimicrobial and insecticidal applications. in the present study, we have focused on the encapsulation of vitex negundo l. leaf essential oil using polysorbate80 for its different biological activities including antioxidant, bactericidal and larvicidal activity against dengue fever vector aedes aegypti l. initially, the nanoemulsion was prepared by low energy method ...201728497330
aedes aegypti piwi4 is a noncanonical piwi protein involved in antiviral responses.the small interfering rna (sirna) pathway is a major antiviral response in mosquitoes; however, another rna interference pathway, the piwi-interacting rna (pirna) pathway, has been suggested to be antiviral in mosquitoes. piwi4 has been reported to be a key mediator of this response in mosquitoes, but it is not involved in the production of virus-specific pirnas. here, we show that piwi4 associates with members of the antiviral exogenous sirna pathway (ago2 and dcr2), as well as with proteins of ...201728497119
efficacy of the vegetative cells of lysinibacillus sphaericus for biological control of insecticide-resistant aedes aegypti.the control of aedes aegypti is usually based on chemical insecticides, but the overuse of these compounds has led to increased resistance. the binary toxin produced by lysinibacillus sphaericus in the final stages of sporulation is used for mosquito control due to its specificity against the culicid larvae; however, it has been proved that ae. aegypti is refractory for this toxin. currently, there is no evidence of the use of l. sphaericus vegetative cells for mosquito biocontrol. therefore, in ...201728490350
assessing the effect of selection with deltamethrin on biological parameters and detoxifying enzymes in aedes aegypti (l.).resistance to insecticides through one or several mechanisms has a cost for an insect in various parameters of its biological cycle. the present study evaluated the effect of deltamethrin on detoxifying enzymes and biological parameters in a population of aedes aegypti selected for 15 generations. the enzyme activities of alpha- and beta-esterases, mixed-function oxidases and glutathione-s-transferases were determined during selection, along with biological parameters.201728485040
synergistic efficacy of aedes aegypti antimicrobial peptide cecropin a2 and tetracycline against pseudomonas aeruginosa.the increasing prevalence of antibiotic resistance has created an urgent need for alternative drugs with new mechanisms of action. antimicrobial peptides (amps) are promising candidates that could address the spread of multidrug-resistant bacteria, either alone or in combination with conventional antibiotics. we studied the antimicrobial efficacy and bactericidal mechanism of cecropin a2, a 36-residue α-helical cationic peptide derived from aedes aegypti cecropin a, focusing on the common pathog ...201728483966
transposition of mutator-like transposable elements (mules) resembles hat and transib elements and v(d)j recombination.mutator-like transposable elements (mules) are widespread across fungal, plant and animal species. despite their abundance and importance as genetic tools in plants, the transposition mechanism of the mule superfamily was previously unknown. discovery of the muta1 element from aedes aegypti and its successful transposition in yeast facilitated the characterization of key steps in muta1 transposition. here we show that purified transposase binds specifically to the muta1 ends and catalyzes excisi ...201728482040
what does heat tell a mosquito? characterization of the orientation behaviour of aedes aegypti towards heat sources.the use of heat as a cue for the orientation of haematophagous insects towards hot-blooded hosts has been acknowledged for many decades. in mosquitoes, thermoreception has been studied at the molecular, physiological and behavioural levels, and the response to heat has been evaluated in multimodal contexts. however, a direct characterization of how these insects evaluate thermal sources is still lacking. in this study we characterize aedes aegypti thermal orientation using a simple dual choice p ...201728479122
eco-friendly synthesis of silver nanoparticles and its larvicidal property against fourth instar larvae of aedes aegypti.in this study, larvicidal activity of silver nanoparticles (agnps) synthesised using apple extract against fourth instar larvae of aedes aegypti was determined. as a result, the agnps showed moderate larvicidal effects against ae. aegypti larvae (lc50 = 15.76 ppm and lc90 = 27.7 ppm). in addition, comparison of larvicidal activity performance of agnps at high concentration prepared using two different methods showed that ae. aegypti larvae was fully eliminated within the duration of 2.5 h. from ...201728476997
larvicidal activity of lignans and alkaloid identified in zanthoxylum piperitum bark toward insecticide-susceptible and wild culex pipiens pallens and aedes aegypti.the yellow fever mosquito, aedes aegypti, and the common house mosquito, culex pipiens pallens, transmit dengue fever and west nile virus diseases, respectively. this study was conducted to determine the toxicity of the three lignans (-)-asarinin, sesamin and (+)-xanthoxylol-γ,γ-dimethylallylether (xda), and the alkaloid pellitorine from zanthoxylum piperitum (rutaceae) bark to third-instar larvae from insecticide-susceptible c. pipiens pallens and ae. aegypti as well as wild c. pipiens pallens ...201728472971
comparison of two detailed models of aedes aegypti population dynamics.the success of control programs for mosquito borne diseases can be enhanced by crucial information provided by models of the mosquito populations. models, however, can differ in their structure, complexity and biological assumptions, and these differences impact their predictions. unfortunately, it is typically difficult to determine why two complex models make different predictions because we lack structured side-by-side comparisons of models using comparable parameterization. here we present a ...201628462011
photo-induced and phytomediated synthesis of silver nanoparticles using derris trifoliata leaf extract and its larvicidal activity against aedes aegypti.due to the increased development of resistance of vectors against synthetic insecticides and chemical drugs, plant based insecticides serve as promising biocontrol agents for effective vector control. green approach for the synthesis of nanoparticles has been attained using environmentally safe, non-toxic plant extracts. the present study was aimed to investigate the potent larvicidal activity of silver nanoparticles (agnps) produced by derris trifoliata leaf extract in relation to the various c ...201728460330
clinical features and neurologic complications of children hospitalized with chikungunya virus in honduras.the first case of chikungunya virus in honduras was identified in 2014. the virus has spread widely across honduras via the aedes aegypti mosquito, leading to an outbreak of chikungunya virus (chikv) in 2015 that significantly impacted children. a retrospective chart review of 235 children diagnosed with chikv and admitted to the national autonomous university of honduras hospital escuela (hospital escuela) in tegucigalpa, honduras, was accomplished with patients who were assessed for clinical f ...201728459170
toxicological effects of sphaeranthus indicus linn. (asteraceae) leaf essential oil against human disease vectors, culex quinquefasciatus say and aedes aegypti linn., and impacts on a beneficial mosquito predator.use of environmentally friendly, decomposable natural products for effective vector control has gained considerable momentum in modern society. in this study, essential oil of sphaeranthus indicus (si-eo) was extracted and further phytochemical screening revealed fourteen compounds with prominent peak area percentage of 24.9 and 22.54% in 3,5-di-tert-butyl-4-hydroxybenzaldehyde and benzene,2-(1,1-dimethylethyl)-1,4-dimethoxy, respectively. the si-eo was further evaluated for their larvicidal res ...028455566
[aedes aegypti larval infestation index and identification of awareness, attitudes and practices related to dengue in tire shops in atlántico, colombia].objective to identify the awareness, attitudes and practices related to dengue in owners and workers of tire ships, as well as the levels of mosquito infestation in tire shops in atlántico department - colombia. methods we conducted a descriptive study. the variables were described as percentages and measures of central tendency and dispersion. index of larval infestation and containers were calculated in each of the municipalities studied. we visited and inspected 111 tire shops. 26.1 % (29/111 ...201528453051
a zika virus from america is more efficiently transmitted than an asian virus by aedes aegypti mosquitoes from asia.zika is a mosquito-borne disease associated with neurological disorders that causes an on-going pandemic. the first outbreak was recorded in micronesia in 2007, then in french polynesia in 2014 from which it spread to south america in 2015 and ignited a widespread epidemic. interestingly, zika outbreaks in asia remained of moderate intensity although the virus is circulating. to understand these epidemiological variations, we investigated the entomological determinants of zikv transmission in as ...201728450714
modelling dengue fever risk in the state of yucatan, mexico using regional-scale satellite-derived sea surface temperature.accurately predicting vector-borne diseases, such as dengue fever, is essential for communities worldwide. changes in environmental parameters such as precipitation, air temperature, and humidity are known to influence dengue fever dynamics. furthermore, previous studies have shown how oceanographic variables, such as el niño southern oscillation (enso)-related sea surface temperature from the pacific ocean, influences dengue fever in the americas. however, literature is lacking on the use of re ...201728450208
detecting the impact of temperature on transmission of zika, dengue, and chikungunya using mechanistic models.recent epidemics of zika, dengue, and chikungunya have heightened the need to understand the seasonal and geographic range of transmission by aedes aegypti and ae. albopictus mosquitoes. we use mechanistic transmission models to derive predictions for how the probability and magnitude of transmission for zika, chikungunya, and dengue change with mean temperature, and we show that these predictions are well matched by human case data. across all three viruses, models and human case data both show ...201728448507
juvenile hormone and its receptor methoprene-tolerant promote ribosomal biogenesis and vitellogenesis in the aedes aegypti mosquito.juvenile hormone (jh) controls many biological activities in insects, including development, metamorphosis, and reproduction. in the aedes aegypti mosquito, a vector of dengue, yellow fever, chikungunya, and zika viruses, the metabolic tissue (the fat body, which is an analogue of the vertebrate liver) produces yolk proteins for developing oocytes. jh is important for the fat body to acquire competence for yolk protein production. however, the molecular mechanisms of how jh promotes mosquito rep ...201728446607
species abundance and temporal variation of arbovirus vectors in brownsville, texas.the recent outbreaks of the dengue fever and west nile viruses and the looming threats of the zika and chikungunya viruses highlight the importance of establishing effective, proactive arboviral surveillance in communities at high risk of transmission, such as those on the texas-mexico border. currently, there are no approved human vaccines available for these mosquito-borne diseases, so entomological control and case management are the only known methods for decreasing disease incidence. the pr ...201728444003
vector competence and transovarial transmission of two aedes aegypti strains to zika virus.zika virus (zikv) has become a serious threat to global health since the outbreak in brazil in 2015. additional chinese cases have continuously been reported since the first case of laboratory-confirmed zikv infection in china on 6 february 2016. aedes aegypti is the most important vector for zikv. this study shows that two strains from china exhibit high levels of midgut infection and highly disseminated infection of salivary glands and ovaries. both strains can transmit zikv to infant mice bit ...201728442754
allergens involved in the cross-reactivity of aedes aegypti with other arthropods.cross-reactivity between aedes aegypti and mites, cockroaches, and shrimp has been previously suggested, but the involved molecular components have not been fully described.201728434865
n-aryl-n'-ethyleneaminothioureas effectively inhibit acetylcholinesterase 1 from disease-transmitting mosquitoes.vector control of disease-transmitting mosquitoes by insecticides has a central role in reducing the number of parasitic- and viral infection cases. the currently used insecticides are efficient, but safety concerns and the development of insecticide-resistant mosquito strains warrant the search for alternative compound classes for vector control. here, we have designed and synthesized thiourea-based compounds as non-covalent inhibitors of acetylcholinesterase 1 (ache1) from the mosquitoes anoph ...201728433681
identification of the septate junction protein gliotactin in the mosquito aedes aegypti: evidence for a role in increased paracellular permeability in larvae.septate junctions (sjs) regulate paracellular permeability across invertebrate epithelia. however, little is known about the function of sj proteins in aquatic invertebrates. in this study, a role for the transmembrane sj protein gliotactin (gli) in the osmoregulatory strategies of larval mosquito (aedes aegypti) was examined. differences in gli transcript abundance were observed between the midgut, malpighian tubules, hindgut and anal papillae of a. aegypti, which are epithelia that participate ...201728432154
dengue virus replicates and accumulates in aedes aegypti salivary glands.dengue virus (denv) is an rna virus transmitted among humans by mosquito vectors, mainly aedes aegypti. denv transmission requires viral dissemination from the mosquito midgut to the salivary glands. during this process the virus undergoes several population bottlenecks, which are stochastic reductions in population size that restrict intra-host viral genetic diversity and limit the efficiency of natural selection. despite the implications for virus transmission and evolution, denv replication i ...201728431281
effects of zika virus strain and aedes mosquito species on vector competence.in the western hemisphere, zika virus is thought to be transmitted primarily by aedes aegypti mosquitoes. to determine the extent to which ae. albopictus mosquitoes from the united states are capable of transmitting zika virus and the influence of virus dose, virus strain, and mosquito species on vector competence, we evaluated multiple doses of representative zika virus strains in ae. aegypti and ae. albopictus mosquitoes. virus preparation (fresh vs. frozen) significantly affected virus infect ...201728430564
competence of aedes aegypti, ae. albopictus, and culex quinquefasciatus mosquitoes as zika virus vectors, china.in china, the prevention and control of zika virus disease has been a public health threat since the first imported case was reported in february 2016. to determine the vector competence of potential vector mosquito species, we experimentally infected aedes aegypti, ae. albopictus, and culex quinquefasciatus mosquitoes and determined infection rates, dissemination rates, and transmission rates. we found the highest vector competence for the imported zika virus in ae. aegypti mosquitoes, some sus ...201728430562
allatotropin: a pleiotropic neuropeptide that elicits mosquito immune responses.allatotropins (at) are neuropeptides with pleotropic functions on a variety of insect tissues. they affect processes such as juvenile hormone biosynthesis, cardiac rhythm, oviduct and hindgut contractions, nutrient absorption and circadian cycle. the present work provides experimental evidence that at elicits immune responses in two important mosquito disease vectors, anopheles albimanus and aedes aegypti. hemocytes and an immune-competent mosquito cell line responded to at by showing strong mor ...201728426765
yellow fever from angola and congo: a storm gathers.in common with zika, chikungunya and dengue, yellow fever (yf) is an arthropod-borne flavivirus. it is transmitted between humans and from monkeys by mosquitoes of the aedes aegypti (its principal vector), haemogogus and albopictus varieties. three cycles of transmission may occur: urban; sylvatic; and intermediate. recently, sub-saharan africa has seen the resurgence of this neglected disease. the current yf outbreak in angola began in december 2015 in the capital luanda and by october 2016 the ...201728424031
efficacy of some wearable devices compared with spray-on insect repellents for the yellow fever mosquito, aedes aegypti (l.) (diptera: culicidae).the current zika health crisis in the americas has created an intense interest in mosquito control methods and products. mosquito vectors of zika are of the genus aedes, mainly the yellow fever mosquito, aedes aegypti. l. the use of repellents to alter mosquito host seeking behavior is an effective method for the prevention of mosquito-borne diseases. a large number of different spray-on repellents and wearable repellent devices are commercially available. the efficacies of many repellents are u ...201728423421
detection of a new pyrethroid resistance mutation (v410l) in the sodium channel of aedes aegypti: a potential challenge for mosquito control.the yellow fever mosquito, aedes aegypti, particularly in neotropical regions, is the principal vector of dengue, yellow fever, zika and chikungunya viruses. pyrethroids remain one of the most used insecticides to control aedes mosquitoes, despite the development of pyrethroid resistance in many mosquito populations worldwide. here, we report a brazilian strain of a. aegypti with high levels (approximately 100-60,000 fold) of resistance to both type i and type ii pyrethroids. we detected two mut ...201728422157
habitat and density of oviposition opportunity influences aedes aegypti (diptera: culicidae) flight distance.understanding the dispersal of aedes (aegypti (l.) diptera: culicidae) after consuming a potentially infectious bloodmeal is an important part of controlling the spread of the arboviruses it transmits. because of the impact on abundance, removal of oviposition sites is a key component of vector control. however, source reduction around a case may encourage dispersal of potentially infected vectors. we compare the effect of oviposition site availability on ae. aegypti dispersal behavior within 30 ...201728419273
evidence of zika virus rna fragments in aedes albopictus (diptera: culicidae) field-collected eggs from camaçari, bahia, brazil.a major mosquito-borne viral disease outbreak caused by zika virus (zikv) occurred in bahia, brazil, in 2015, largely due to transmission by the mosquito, aedes aegypti (l.). detecting zikv in field samples of ae. aegypti has proven problematic in some locations, suggesting other mosquito species might be contributing to the spread of zikv. in this study, several (five) adult aedes albopictus (skuse) mosquitoes that emerged from a 2015 field collection of eggs from camaçari, bahia, brazil, were ...201728419254
deploying dengue-suppressing wolbachia : robust models predict slow but effective spatial spread in aedes aegypti.a novel strategy for controlling the spread of arboviral diseases such as dengue, zika and chikungunya is to transform mosquito populations with virus-suppressing wolbachia. in general, wolbachia transinfected into mosquitoes induce fitness costs through lower viability or fecundity. these maternally inherited bacteria also produce a frequency-dependent advantage for infected females by inducing cytoplasmic incompatibility (ci), which kills the embryos produced by uninfected females mated to inf ...201728411063
mycobacterium ulcerans low infectious dose and mechanical transmission support insect bites and puncturing injuries in the spread of buruli ulcer.addressing the transmission enigma of the neglected disease buruli ulcer (bu) is a world health organization priority. in australia, we have observed an association between mosquitoes harboring the causative agent, mycobacterium ulcerans, and bu. here we tested a contaminated skin model of bu transmission by dipping the tails from healthy mice in cultures of the causative agent, mycobacterium ulcerans. tails were exposed to mosquito (aedes notoscriptus and aedes aegypti) blood feeding or punctur ...201728410412
severe dengue due to secondary hemophagocytic lymphohistiocytosis: a case study.dengue, transmitted by the mosquito aedes aegypti affects millions of people worldwide every year. dengue induced hemophagocytic lymphohistiocytosis (hlh) is a serious condition and may prove fatal if not detected early and treated appropriately. diagnosis of hlh is challenging and usually missed as clinical and laboratory findings are nonspecific. moreover, the pathophysiology of the systemic inflammatory response syndrome and/or sepsis is remarkably similar to hlh. secondary hlh following infe ...201728409119
vector competence and innate immune responses to dengue virus infection in selected laboratory and field-collected stegomyia aegypti (= aedes aegypti).control of dengue virus (denv) transmission, primarily based on strategies to reduce populations of the principle vector stegomya aegypti (= aedes aegypti) (diptera: culicidae), is difficult to sustain over time. other potential strategies aim to manipulate characteristics such as vector competence (vc), the innate capacity of the vector to transmit the virus. previous studies have identified genetic factors, including differential expression of apoptosis-related genes, associated with the refra ...201728407282
chemosensory responses to the repellent nepeta essential oil and its major component nepetalactone by aedes aegypti (diptera: culicidae), a vector of zika virus.nepeta essential oil (neo; catnip) and its major component, nepetalactone, have long been known to repel insects including mosquitoes. however, the neural mechanisms through which these repellents are detected by mosquitoes, including the yellow fever mosquito aedes aegypti (l.), an important vector of zika virus, were poorly understood. here we show that neo volatiles activate olfactory receptor neurons within the basiconic sensilla on the maxillary palps of female ae. aegypti. a gustatory rece ...201728407077
quantitative proteomic analysis of mosquito c6/36 cells reveals host proteins involved in zika virus infection.zika virus (zikv) is an emerging arbovirus belonging to the genus flavivirus of the family flaviviridae during replication processes, flavivirus manipulates host cell systems to facilitate its replication, while the host cells activate antiviral responses. identification of host proteins involved in the flavivirus replication process may lead to the discovery of antiviral targets. the mosquitoes aedes aegypti and aedes albopictus are epidemiologically important vectors for zikv, and effective re ...201728404849
advances in vector control science: rear-and-release strategies show promise… but don't forget the basics.both chikungunya and zika viruses have recently swept from africa across the pacific to the americas, causing major outbreaks of disease in humans. in the meantime, dengue epidemics continue throughout the tropics. traditional vector control programs based on strategies from 1950s and 1960s have been relatively ineffective in combating recent epidemics. in response, new methods involving the rearing and releasing of large numbers of mosquitoes to eliminate or modify local aedes populations are b ...201728403439
the impact of temperature and body size on fundamental flight tone variation in the mosquito vector aedes aegypti (diptera: culicidae): implications for acoustic lures.aedes aegypti (l.) males use female flight tone as a means of mate localization. by playing the sound of a flying female, males can be attracted to a trap to monitor mosquito populations and the progress of transgenic male releases. however, the female flight tone used to attract males needs to be optimized to maximize trap effectiveness. the fundamental frequency of female flight tone could be influenced by both body size and ambient temperature. however, no analysis yet has considered both the ...201728402550
aedes aegypti (diptera: culicidae) abundance model improved with relative humidity and precipitation-driven egg hatching.we propose an improved aedes aegypti (l.) abundance model that takes into account the effect of relative humidity (rh) on adult survival, as well as rainfall-triggered egg hatching. the model uses temperature-dependent development rates described in the literature as well as documented estimates for mosquito survival in environments with high rh, and for egg desiccation. we show that combining the two additional components leads to better agreement with surveillance trap data and with dengue inc ...201728402546
repellency of 29 synthetic and natural commercial topical insect repellents against aedes aegypti (diptera: culicidae) in central mexico.in mexico, the use of repellents to prevent insects from landing and biting is a common practice. however, variation in the efficiency of natural and synthetic repellents has been observed. in this study, we evaluated the repellency and protection time of 16 synthetic and 13 natural-based commercial products against aedes aegypti (l.) from an endemic dengue area (jojutla, morelos) in central mexico. the "arm exposure" cage test was used to assess the efficacy of the repellents. tests were conduc ...201728402436
why is aedes aegypti linnaeus so successful as a species?diseases transmitted by mosquitoes impose enormous burden towards human morbidity and mortality. over the last three decades, brazil has suffered from severe dengue epidemics. in september 2014, this situation is further complicated by the introduction of two other viruses, zika and chikungunya, placing brazil in a triple epidemic. in this article, we discuss the biology of aedes aegypti linnaeus, and the principal initiatives currently used to control mosquito populations and the diseases they ...201728401481
immunohistochemical mapping and transcript expression of the gpa2/gpb5 receptor in tissues of the adult mosquito, aedes aegypti.gpa2/gpb5 is a glycoprotein hormone found in most bilateral metazoans including the mosquito, aedes aegypti. to elucidate physiological roles and functions of gpa2/gpb5, we aim to identify prospective target tissues by examining the tissue- and sex-specific expression profile of its receptor, the leucine-rich repeat-containing g protein-coupled receptor 1 (lgr1) in the adult mosquito. western analyses using a heterologous system with cho-k1 cells, transiently expressing a. aegypti lgr1, yielded ...201728401307
wingbeat frequency-sweep and visual stimuli for trapping male aedes aegypti (diptera: culicidae).combinations of female wingbeat acoustic cues and visual cues were evaluated to determine their potential for use in male aedes aegypti (l.) traps in peridomestic environments. a modified centers for disease control (cdc) light trap using a 350-500 hz frequency-sweep broadcast from a speaker as an acoustic stimulus, combined with a black poster-board half-cylinder behind the trap as a visual stimulus, captured a significantly greater proportion of males in a laboratory arena during daylight than ...201728399312
effect of temperature thresholds on modeled aedes aegypti (diptera: culicidae) population dynamics.dynamic simulation models provide vector abundance estimates using only meteorological data. however, model outcomes may heavily depend on the assumptions used to parameterize them. we conducted a sensitivity analysis for a model of aedes aegypti (l.) abundance using weather data from two locations where this vector is established, la margarita, puerto rico and tucson, arizona. we tested the effect of simplifying temperature-dependent development and mortality rates and of changing development a ...201728399306
factors of concern regarding zika and other aedes aegypti-transmitted viruses in the united states.the recent explosive outbreaks of zika and chikungunya throughout the americas has raised concerns about the threats that these and similar diseases may pose to the united states (u.s.). the commonly accepted association between tropical climates and the endemicity of these diseases has led to concerns about the possibility of their redistribution due to climate change and transmission arising from cases imported from endemic regions initiating outbreaks in the united states. while such possibil ...201728399294
repellent and larvicidal activity of the essential oil from eucalyptus nitens against aedes aegypti and aedes albopictus (diptera: culicidae).dengue, chikungunya, and yellow fever are important vector-borne diseases transmitted by female mosquitoes when they feed on humans. the use of repellents based on natural products is an alternative for personal protection against these diseases. application of chemicals with larvicidal activity is another strategy for controlling the mosquito population. the repellent and larvicidal activities of the essential oil from eucalyptus nitens were tested against aedes aegypti and aedes albopictus, th ...201728399283
transcriptional profile for detoxification enzymes aeaggt1 and aaeggt2 from aedes aegypti (diptera: culicidae) in response to larvicides.aedes aegypti (l.) is the vector responsible for transmitting dengue, chikungunya, yellow fever, and zika viruses, as well as other pathogens. microbial larvicides based on bacillus thuringiensis berliner israelensis (bti) and saccharopolyspora spinosa mertz and yao, such as vectobac 12as and natular 2ec, have been shown to be effective in reducing larval populations of ae. aegypti. we examined the gene expression of two detoxification enzymes, glucosyl and glucuronosyl transferases (aaeggt1 and ...201728399278
detection and establishment of aedes aegypti and aedes albopictus (diptera: culicidae) mosquitoes in california, 2011-2015.in 2011, a thriving population of aedes albopictus (skuse), the asian tiger mosquito, was discovered within three cities in los angeles county over an estimated 52-km2 urban area. two years later in 2013, aedes aegypti (l.), the yellow fever mosquito, was detected within several urban areas of madera, fresno, and san mateo counties. state and local vector control agencies responded with an aggressive effort to eradicate or interrupt the spread of these two invasive mosquitoes; however, known pop ...201728399270
ecological niche model for predicting distribution of disease-vector mosquitoes in yucatán state, méxico.the majority of the yucatán state, méxico, presents subtropical climate that is suitable for many species of mosquitoes that are known to be vectors of diseases, including those from the genera aedes and culex. the objective of this study is to identify the geographic distribution of five species from these two genera and estimate the human population at risk of coming in contact with them. we compiled distributional data for aedes aegypti (l.), aedes (howardina) cozumelensis (diaz najera), cule ...201728399263
emergent and reemergent arboviruses in south america and the caribbean: why so many and why now?several arboviruses have emerged and/or reemerged in the new world in the past decades. zika and chikungunya viruses, formerly restricted to africa and perhaps asia, invaded the continent, causing great concern. dengue virus outbreaks have continued to occur in almost all countries, with millions of cases per year. west nile virus rapidly invaded north america, and now cases have been found in central and south america. other arboviruses, such as mayaro and eastern equine encephalitis viruses, h ...201728399216
eleutherococcus senticosus (araliaceae) leaf morpho-anatomy, essential oil composition, and its biological activity against aedes aegypti (diptera: culicidae).the roots of eleutherococcus senticosus (rupr. & maxim.) maxim., a well-known medicinal plant from eastern asia, are used worldwide for their known beneficial medicinal properties. recently, the leaves have been used as an alternative to the roots. the present study was aimed at exploring the leaf essential oil as a potential source of compounds for mosquito management. gas chromatography/mass spectrometry analysis of the leaf essential oil revealed 87 compounds, constituting 95.2% of the oil. α ...201728399215
a video-tracking analysis-based behavioral assay for larvae of anopheles pseudopunctipennis and aedes aegypti (diptera: culicidae).aedes aegypti (l.) is the primary vector of dengue, yellow fever, zika, and chikungunya viruses, whereas anopheles pseudopunctipennis (theobald) is the principal vector for malaria in latin america. the larval stage of these mosquitoes occurs in very different development habitats, and the study of their respective behaviors could give us valuable information to improve larval control. the aim of this study was to set up a bioassay to study basic larval behaviors using a video-tracking software. ...201728399206
differential pathogenicity of metarhizium blastospores and conidia against larvae of three mosquito species.biorational insecticides are being increasingly used in integrated pest management programs. in laboratory bioassays, the pathogenicity of blastospores and conidia of the entomopathogenic fungus metarhizium brunneum arsef 4556 was evaluated against larvae of three mosquito species. three propagule concentrations (1 × 106, 1 × 107, and 1 × 108 spores ml - 1) were used in the bioassays. results showed that aedes aegypti had lower survival rates when exposed to blastospores than when exposed to con ...201728399202
[zika virus infection in pregnancy].the zika virus is a flavivirus spread by mosquitoes. its primary vectors are the aedes aegypti and the aedes albopictus. before 2007 it sporadically caused benign morbidity. since 2015, it started spreading "explosively" in america, especially in brazil. in august 2016 they reported cases from new york and poland, too. most of the infections don't produce any symptoms, but can cause grave complications. the most important lesion is microcephalia that forms in fetuses. microcephalia's most seriou ...201728393598
epidemiological characteristics of dengue disease in latin america and in the caribbean: a systematic review of the literature.dengue, an important mosquito-borne virus transmitted mainly by aedes aegypti, is a major public health issue in latin america and the caribbean. national epidemiological surveillance systems, usually based on passive detection of symptomatic cases, while underestimating the true burden of dengue disease, can provide valuable insight into disease trends and excess reporting and potential outbreaks. we carried out a systematic review of the literature to characterize the recent epidemiology of de ...201728392806
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