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cocos nucifera coir-mediated green synthesis of pd nps and its investigation against larvae and agricultural pest.in recent decades, several scientists focused their process towards nanoparticles synthesis by using various sustainable approaches. cocos nucifera (c. nucifera) was one of the versatile trees in tropical regions which also can act as a thrust quencher in all over the world. cocos nucifera coir was one of the waste by-products in all coconut-refining industries and with the help c. nucifera coir, palladium nanoparticles (pd nps) were synthesized. green-synthesized spherical-shape pd nps were ove ...201627908201
larvicidal activity of green synthesized silver nanoparticles using excoecaria agallocha l. (euphorbiaceae) leaf extract against aedes aegypti.green synthesis of silver nanoparticles (agnps) using plant extracts has been achieved by eco-friendly reducing and capping agents. the present study was conducted to evaluate the larvicidal efficacies of agnps synthesized using aqueous leaf extracts of excoecaria agallocha against dengue vector, aedes aegypti. the 3(rd) and 4(th) instar larvae of a. aegypti were exposed to various concentrations of aqueous extracts of e. agallocha, synthesized agnps and also crude solvent extracts (methanol and ...201627906138
spatial pattern evolution of aedes aegypti breeding sites in an argentinean city without a dengue vector control programme.the main objective of this study was to obtain and analyse the space-time dynamics of aedes aegypti breeding sites in clorinda city, formosa province, argentina coupled with landscape analysis using the maximum entropy approach in order to generate a dengue vector niche model. in urban areas, without vector control activities, 12 entomologic (larval) samplings were performed during three years (october 2011 to october 2014). the entomologic surveillance area represented 16,511 houses. predictive ...201627903056
wolbachia suppresses cell fusing agent virus in mosquito cells.the genus flavivirus contains a large number of positive-sense ssrna viruses. while some are transmitted by mosquitoes or other arthropods and are pathogenic to humans and animals (e.g. dengue and zika viruses), some are insect-specific and do not replicate in vertebrate cells. these are known as insect-specific flaviviruses (isfs). cell fusing agent virus (cfav) was the first described isf, which was detected in an aedes aegypti cell line, aag2. here, we investigated the effect of wolbachia, a ...201627902358
fern-synthesized silver nanocrystals: towards a new class of mosquito oviposition deterrents?mosquitoes act as vectors of devastating pathogens and parasites, representing a key threat for millions of humans and animals worldwide. eco-friendly control tools are urgently required. we proposed a novel method of fern-mediated biosynthesis of silver nanoparticles (agnp) using dicranopteris linearis, acting as a reducing and capping agent. agnp were characterized by uv-vis spectroscopy, fourier transform infrared (ftir) spectroscopy, scanning electron microscopy (sem), energy-dispersive x-ra ...201627892872
methods and procedures used in aedes aegypti control in the successful campaign for yellow fever prophylaxis in rio de janeiro, brazil, in 1928 and 1929.to review the challenges and procedures used in aedes aegypti control and the characteristics of the epidemics in the successful campaign, led by clementino fraga, for yellow fever control in rio de janeiro, brazil, in 1928 and 1929, before the vaccine (1937) and ddt (1947).201627869977
incidence of dengue cases (2007-2013) and seasonal distribution of mosquitoes (diptera: culicidae) (2012-2013) in barreiras, bahia, brazil.to describe seasonal mosquito distribution and the incidence of dengue cases in barreiras, bahia, brazil.201627869967
[aedes aegypti control strategies: a review].to describe the main strategies to control aedes aegypti, with emphasis on promising technological innovations for use in brazil.201627869956
no magic bullet: citizenship and social participation in the control of aedes aegypti. 201627869933
[first report of the f1534c mutation associated with cross-resistance to ddt and pyrethroids in aedes aegypti from colombia].the main strategy for the control of aedes aegypti, vector of dengue, chikungunya and zika viruses, is based on the use of insecticides to reduce its populations. however, their use has led to insect resistance to these chemicals. objective: to determine the presence of the f1534c mutation associated with cross-resistance to ddt and pyrethroids in a. aegypti in sincelejo, colombia. materials and methods: we studied nine specimens of a. aegypti that showed resistance to lambdacyhalothrin in bioas ...201627869391
housing improvement: a novel paradigm for urban vector-borne disease control? 201627864518
epidemiological characteristics of yellow fever in brazil, 2000-2012.this study aims to describe the epidemiological characteristics of yellow fever in brazil in the period 2000-2012.201627861674
movement of aedes aegypti following a sugar meal and its implication in the development of control strategies in durán, ecuador.we evaluated how the presence of sugar sources impacted the distribution of aedes aegypti in different habitats in durán, ecuador. land cover and normalized difference vegetation index maps were used to guide a random point sampling routine to select study grids (30 m × 30 m) in low vegetation (lv) and high vegetation (hv). five individual plants, at one home in the lv and hv grid, were treated with a different colored, non-attractive, 60% sucrose solution to determine mosquito feeding and movem ...201627860016
evaluation of collection methods for culex quinquefasciatus, aedes aegypti, and aedes simpsoni in northeastern tanzania.in east africa, significant morbidity and mortality are caused by infections spread by culex quinquefasciatus and aedes aegypti. sticky traps have been shown to be effective tools for sampling populations of aedes mosquitoes and have been found to catch cx. quinquefasciatus. thus, they could potentially be used to sample populations of this species. this study compared sticky ovitraps (so) and mosquitraps (mqt) with the cdc gravid trap (cdc-gt) for collection of culex and aedes mosquito populati ...201627860009
green synthesis of selenium nanoparticles conjugated clausena dentata plant leaf extract and their insecticidal potential against mosquito vectors.mosquitoes are major vectors for the transmission of many diseases like chikungunya, malaria, dengue, zika, etc. worldwide. in the present study, selenium nanoparticles (senps) were synthesized from clausena dentata and were tested for their larvicidal efficacy against the fourth-instar larvae of anopheles stephensi, aedes aegypti, and culex quinquefasciatus. the synthesized nanoparticles were characterized using uv-vis spectroscopy, fourier transform infrared radiation (ftir) spectroscopy, edax ...201627832715
cuba: time to expand health in all policies.the adelaide statement on health in all policies (2010), lays out equity-based principles designed to guide policymakers on incorporating health and well-being components into the development, implementation and evaluation of policy and practice while moving towards shared governance at all levels-local, regional, national and international. special emphasis is placed on cross-sector coordination to achieve policy goals, while improving health and well-being for all.[1] in cuba's case, experienc ...201627829655
collagen extract from marine finfish scales as a potential mosquito larvicide.collagen is a peptide being utilized in medical, health care, nutrient and decorative industry. marine fish scales are one of the good sources of collagen, which is extracted using the advanced enzymatic digestion method. scales of sardinella longiceps (oil sardine) have a high proportion of collagen. this product is well absorbed with broad adaptive values that encourage the inclusion of nutriments. in this paper, we have performed the isolation and characterization of collagen from s. longicep ...201627804059
controlling aedes aegypti in cryptic environments with manually carried ultra-low volume and mist blower pesticide applications.because aedes aegypti , a vector of dengue, chikungunya, and zika viruses, exhibits resting and reproductive behaviors that present challenges to control them, pesticide application equipment available for vector control must be evaluated for their ability to control this species in a variety of cryptic environments. five types of pesticide sprayers, representing 3 spray technologies (1 mister, 2 ultra-low volume [ulv] cold foggers, and 2 ulv thermal foggers), were evaluated for their ability to ...201627802406
operational aspects of the centers for disease control and prevention autocidal gravid ovitrap.dengue viruses cause hundreds of millions of infections every year in tropical and subtropical countries. unfortunately, there is not a single universal vector control method capable of suppressing aedes aegypti (l.) populations. amongst novel control tools or approaches are various types of traps targeting gravid females or their eggs. here, we provide details of the operational use of the centers for disease control and prevention autocidal gravid ovitrap (cdc-ago trap) for the surveillance an ...201627802402
comparative excito-repellency of three cambodian plant-derived extracts against two mosquito vector species, aedes aegypti and anopheles minimus.a study of the behavioral responses of aedes aegypti and anopheles minimus to 3 cambodian plant extracts at 3 different concentrations (1%, 2.5%, and 5%) was performed using an excito-repellency test system. these 3 plants were strophanthus scandens, capparis micracantha, and dioscorea hispida, selected according to traditional healer's knowledge, bibliographic studies and market surveys. results showed that s. scandens leaves' hexane extract was the only one to exert repellency against ae. aegy ...201627802401
evaluation of a noncontact, alternative mosquito repellent assay system.a novel noncontact repellency assay system (ncras) was designed and evaluated as a possible alternative method for testing compounds that repel or inhibit mosquitoes from blood feeding. deet and aedes aegypti were used in a controlled laboratory setting. using 2 study designs, a highly significant difference were seen between deet-treated and untreated skin placed behind the protective screens, indicating that deet was detected and was acting as a deterrence to mosquito landing and probing behav ...201627802399
morphological differentiation of the eggs of north american container-inhabiting aedes mosquitoes.aedes aegypti , ae. albopictus, ae. japonicus japonicus, and ae. triseriatus eggs are described with the aid of color images and images from scanning electron microscopy. all eggs were broadly cigar shaped with ae. triseriatus and ae. j japonicus eggs being dull or matte black, while ae. albopictus and ae. aegypti eggs were shiny jet black. aedes triseriatus eggs were lighter in color and had a rougher appearance when compared to those of ae. j. japonicus. aedes albopictus and ae. aegypti eggs c ...201627802396
mosquitoes host communities of bacteria that are essential for development but vary greatly between local habitats.mosquitoes are insects of interest because several species vector disease-causing pathogens to humans and other vertebrates. we previously reported that mosquitoes from long-term laboratory cultures require living bacteria in their gut to develop, but development does not depend on particular species of bacteria. here, we focused on three distinct but interrelated areas of study to better understand the role of bacteria in mosquito development by studying field and laboratory populations of aede ...201627718295
light-driven processes control both rhodopsin maturation and recycling in mosquito photoreceptors.many invertebrates carry out a daily cycle of shedding and rebuilding of the photoreceptor's photosensitive rhabdomeric membranes. the mosquito aedes aegypti shows a robust response, losing nearly all aaop1 rhodopsin from the rhabdomeric membranes during the shedding process at dawn. here, we made use of aaop1 antibodies capable of distinguishing newly synthesized, glycosylated rhodopsin from mature nonglycosylated rhodopsin to characterize the fate of aaop1 during the shedding and rebuilding pr ...201627798185
melaleuca quinquenervia (cav.) s.t. blake (myrtales: myrtaceae): natural alternative for mosquito control.to evaluate an essential oil with larvicide, adulticide and growth inhibitory activity against aedes aegypti, aedes albopictus and culex quinquefasciatus mosquitoes, of medical importance.201627794392
identification and analysis of a processed cytochrome p450 pseudogene of the disease vector aedes aegypti.to clone cytochrome p450 from aedes aegypti (ae. aegypti) and determine the characteristics using bioinformatics tools.201627794391
global genetic diversity of aedes aegypti.mosquitoes, especially aedes aegypti, are becoming important models for studying invasion biology. we characterized genetic variation at 12 microsatellite loci in 79 populations of ae. aegypti from 30 countries in six continents, and used them to infer historical and modern patterns of invasion. our results support the two subspecies ae. aegypti formosus and ae. aegypti aegypti as genetically distinct units. ae. aegypti aegypti populations outside africa are derived from ancestral african popula ...201627671732
identification and expression analysis of two 14-3-3 proteins in the mosquito aedes aegypti, an important arboviruses vector.the 14-3-3 proteins are evolutionarily conserved acidic proteins that form a family with several isoforms in many cell types of plants and animals. in invertebrates, including dipteran and lepidopteran insects, only two isoforms have been reported. 14-3-3 proteins are scaffold molecules that form homo- or heterodimeric complexes, acting as molecular adaptors mediating phosphorylation-dependent interactions with signaling molecules involved in immunity, cell differentiation, cell cycle, prolifera ...201627592842
dengue in a changing climate.dengue is the world's most important arboviral disease in terms of number of people affected. over the past 50 years, incidence increased 30-fold: there were approximately 390 million infections in 2010. globalization, trade, travel, demographic trends, and warming temperatures are associated with the recent spread of the primary vectors aedes aegypti and aedes albopictus and of dengue. overall, models project that new geographic areas along the fringe of current geographic ranges for aedes will ...201627475051
[translation into practice of empowerment strategies for dengue prevention: facilitators and barriers].objective to identify facilitators and barriers to the process of translating community empowerment strategies into the practice of the national aedes aegypti control program in cuba. methods a case study of the process was conducted from 2005 to 2009 at national and provincial levels of the control program. data from participant observation, in-depth interviews, and document analysis were triangulated and analyzed inductively and deductively. results the main facilitators identified included a ...201627754520
isolation and identification of mosquito (aedes aegypti) biting-deterrent compounds from the native american ethnobotanical remedy plant hierochloë odorata (sweetgrass).hierochloë odorata (l.) p. beauv. (poaceae), commonly known as sweetgrass, has documented use as an insect repellent by the flatheads of montana and blackfoot of alberta. both the flatheads of montana and blackfoot of alberta would use braided plant material in a sachet in clothing or burn them from one end as incense, air/clothing freshener, and insect repellent. this study evaluated the insect-repellent properties of this plant using an in vitro mosquito aedes aegypti feeding bioassay-directed ...201627744691
dengue related osteonecrosis of the maxillary dento-alveolar bone.dengue is a mosquito transmitted flaviviral infection which can give rise to severe haemorrhage (dengue haemorrhagic fever/dhf) and with capillary leakage induces hypovolemic shock (dengue shock syndrome/dss). although dengue symptoms and complications have been known for many decades there has only been one documented case of osteonecrosis of the maxilla which was treated by excision of the necrotic bone. in this case of dengue infection, extensive maxillary osteonecrosis and minimal root resor ...201627743399
pyrethroid resistance in aedes aegypti and aedes albopictus: important mosquito vectors of human diseases.aedes aegypti and a. albopictus mosquitoes are vectors of important human disease viruses, including dengue, yellow fever, chikungunya and zika. pyrethroid insecticides are widely used to control adult aedes mosquitoes, especially during disease outbreaks. herein, we review the status of pyrethroid resistance in a. aegypti and a. albopictus, mechanisms of resistance, fitness costs associated with resistance alleles and provide suggestions for future research. the widespread use of pyrethroids ha ...201627742355
discovery of selective inhibitors targeting acetylcholinesterase 1 from disease-transmitting mosquitoes.vector control of disease-transmitting mosquitoes is increasingly important due to the re-emergence and spread of infections such as malaria and dengue. we have conducted a high throughput screen (hts) of 17,500 compounds for inhibition of the essential ache1 enzymes from the mosquitoes anopheles gambiae and aedes aegypti. in a differential hts analysis including the human ache, several structurally diverse, potent, and selective noncovalent ache1 inhibitors were discovered. for example, a pheno ...201627598521
proteomic analysis of aedes aegypti midgut during post-embryonic development and of the female mosquitoes fed different diets.in this work we analyzed protein expression in the aedes aegypti midgut during the larval (fourth instar, l4), pupal, and adult stages [including newly emerged (ne), sugar-fed (sf) and blood-fed (bf) females]. two-dimensional electrophoresis showed 13 spots in the midgut of larvae, 95 in the midgut of pupae, 90 in the midgut of ne, and 76 in the midgut of sf or bf females. in the larval midguts, high serpin expression was noted, while in the pupae, protein abundance was lower than in the ne, sf, ...201627597118
egg hatching and survival of immature stages of aedes aegypti (diptera: culicidae) under natural temperature conditions during the cold season in buenos aires, argentina.in temperate regions, the seasonal dynamics of aedes aegypti (l.) (diptera: culicidae) is mainly influenced by temperature. it is assumed that, during the winter season, the population remains as eggs and that the development and population growth of surviving eggs begin during the following spring. the aim of the current study was to assess egg hatching of ae. aegypti during the winter in buenos aires city (argentina), and analyze the survival of immature stages. the experiments consisted of im ...201627576509
mosquito c-type lectins maintain gut microbiome homeostasis.the long-term evolutionary interaction between the host immune system and symbiotic bacteria determines their cooperative rather than antagonistic relationship. it is known that commensal bacteria have evolved a number of mechanisms to manipulate the mammalian host immune system and maintain homeostasis. however, the strategies employed by the microbiome to overcome host immune responses in invertebrates still remain to be understood. here, we report that the gut microbiome in mosquitoes utilize ...201627572642
toxicity and larvicidal activity of podophyllum-based lignans against aedes aegypti (diptera: culicidae).aedes aegypti l. (diptera: culicidae) is a mosquito species that has adapted to urban environments and is the main vector of dengue viruses. because of the increasing incidence of dengue, a more environmentally acceptable insecticide needs to be found. natural products have been and continue to be an important source of leading compounds that can be modified in order to develop new drugs. the lignan family of natural products includes compounds with a diverse spectrum of biological activity. pod ...201627561675
eggs viability of aedes aegypti linnaeus (diptera, culicidae) under different environmental and storage conditions in manaus, amazonas, brazil.the viability of aedes aegypti eggs was assessed in the amazon region. the eggs were maintained under different conditions: indoors (insectarium) and outdoors (natural environment), as well as in different storage types (plastic cup, paper envelope, plastic bag) for different days. egg viability was measured as the mean of hatchings observed from egg-bearing sheets of filter paper immersed in water, using three sheets randomly selected from each storage type and at both sites. there were signifi ...201627533732
determination of juvenile hormone titers by means of lc-ms/ms/ms and a juvenile hormone-responsive gal4/uas system in aedes aegypti mosquitoes.in anautogenous mosquitoes, juvenile hormone iii (jh) plays an essential role in female post-eclosion (pe) development, preparing them for subsequent blood feeding and egg growth. we re-examined the jh titer during the reproductive cycle of female aedes aegypti mosquitoes. using liquid chromatography coupled with triple tandem mass spectrometry (lc-ms/ms/ms), we have shown that it reaches its peak at 48-54 h pe in the female hemolymph and at 72 h pe in whole body extracts. this method represents ...201627530057
aedes aegypti suppression in the americas: historical perspectives. 201627529102
phylogenetic analysis of the atp-binding cassette transporter family in three mosquito species.the atp-binding cassette (abc) transporter family functions in the atp-dependent transportation of various substrates across biological membranes. abc proteins participate in various biological processes and insecticide resistance in insects, and are divided into eight subfamilies (a-h). mosquitoes are important vectors of human diseases, but the mechanism by which the abc transporter family evolves in mosquitoes is unknown. in this study, we classified and compared the abc transporter families ...201627521922
solution nuclear magnetic resonance studies of sterol carrier protein 2 like 2 (scp2l2) reveal the insecticide specific structural characteristics of scp2 proteins in aedes aegypti mosquitoes.sterol carrier protein 2 like 2 from aedes aegypti (aescp2l2) plays an important role in lipid transport in mosquitoes for its routine metabolic processes. repeated unsuccessful attempts to crystallize ligand free scp2l2 prompted us to undertake nuclear magnetic resonance (nmr) spectroscopy to determine its three-dimensional structure. we report here the three-dimensional structures and dynamics of apo-aescp2l2 and its complex with palmitate. the (15)n heteronuclear single-quantum coherence spec ...201627508310
conidiobolus macrosporus (entomophthorales), a mosquito pathogen in central brazil.a new fungal pathogen of culicinae (diptera: culicidae) adults, conidiobolus macrosporus (entomophthorales: ancylistaceae), was detected and isolated during a survey of mosquito pathogens close to the city of aruanã, goiás state, in december 2014. the morphological characteristics of c. macrosporus are presented, and reasons for some uncertainty about this identification are discussed. the pathogenicity and high virulence of this fungus for aedes aegypti were confirmed in laboratory conditions. ...201627506454
identification of aacasps7, an effector caspase in aedes albopictus.aedes albopictus mosquito is a vector of various arboviruses and is becoming a significant threat to public health due to its rapid global expansion. several reports suggest that apoptosis could be a factor limiting arbovirus infection in mosquitoes. thus, it is significant to identify apoptosis pathway and study the correlation between apoptosis and virus infection in mosquitoes. apoptosis is a type of programmed cell death that plays a vital role in immunity, development, and tissue homeostasi ...201627502418
larvicidal activity of natural and modified triterpenoids against aedes aegypti (diptera: culicidae).insecticide resistance to commonly used substances demands new molecules for the chemical control of the dengue vector aedes aegypti. because natural product sources have been an alternative to obtain larvicidal compounds, the aim of this study was to evaluate the triterpenoids betulinic (ba) and ursolic (ua) acids and their semi-synthetic derivatives against larval ae. aegypti. ba, ua, ten derivatives modified at the c-3 position and a positive control (diflubenzuron) were evaluated. larvicidal ...201627501778
coexistence of aedes aegypti and aedes albopictus (diptera: culicidae) in peninsular florida two decades after competitive displacements.the spread of aedes albopictus (skuse) eastward in the mid-1980s from its initial establishment in houston, tx, was associated with rapid declines and local disappearances of aedes aegypti (l.) in gulf coast states and florida where annual larval surveillance during the early 1990s described temporal and spatial patterns of competitive displacements in cemeteries and tire shops. approximately 20 yr later in 2013-2014, we re-visited former collection sites and sampled aquatic immatures of these t ...201627493253
microrna-309 targets the homeobox gene six4 and controls ovarian development in the mosquito aedes aegypti.obligatory blood-triggered reproductive strategy is an evolutionary adaptation of mosquitoes for rapid egg development. it contributes to the vectorial capacity of these insects. therefore, understanding the molecular mechanisms underlying reproductive processes is of particular importance. here, we report that microrna-309 (mir-309) plays a critical role in mosquito reproduction. a spatiotemporal expression profile of mir-309 displayed its blood feeding-dependent onset and ovary-specific manife ...201627489347
the sex locus is tightly linked to factors conferring sex-specific lethal effects in the mosquito aedes aegypti.in many taxa, sex chromosomes are heteromorphic and largely non-recombining. evolutionary models predict that spread of recombination suppression on the y chromosome is fueled by the accumulation of sexually antagonistic alleles in close linkage to the sex determination region. however, empirical evidence for the existence of sexually antagonistic alleles is scarce. in the mosquito aedes aegypti, the sex-determining chromosomes are homomorphic. the region of suppressed recombination, which surro ...201627485667
ecotoxicological assessment of pyriproxyfen under environmentally realistic exposure conditions of integrated vector management for aedes aegypti control in brazil.there is increasing concern to control aedes aegypti mosquito exposure in developing countries such as brazil. thus, integrated approaches using a combination of chemical, pyriproxyfen larvicide, and biological, xiphophorus maculatus, larvivorous fish species approaches are necessary and important to initiate more effective control against mosquito borne diseases. this study describes the toxicological effects of pyriproxyfen larvicide on the fish xiphophorus maculatus, the larvivorous fish spec ...201627458879
a novel in vitro bioassay to explore the repellent effects of compounds against mosquito aedes aegypti (diptera: culicidae).mosquitoes are vectors for many pathogens resulting in many deaths of humans. repellents play an important role in reducing mosquito bites and the spread of mosquito-borne diseases. currently, klun & debboun (k & d) and human-arm-based bioassay systems are used to identify repellent properties of compounds, extracts, and essential oils. risks involved with human-arm-based systems are allergic reactions and limited replicates. we are reporting an in vitro bioassay method “ncnpr repellent bioassay ...201626590191
development of a mosquito attractant blend of small molecules against host-seeking aedes aegypti.a mosquito's dependence on olfaction in the hunt for human host could be efficiently exploited to protect humans from mosquito bites. the present study is undertaken to make the most attractant compound blend for aedes aegypti mosquitoes to lure them to traps. eleven molecules (m1-m11) at different dilutions were screened for attractancy against non-blood-fed adult female mosquitoes in an olfactometer. the results showed that the attractancy was dependent on both the chemical nature of the molec ...201626693718
mosquito salivary allergen aed a 3: cloning, comprehensive molecular analysis, and clinical evaluation.allergic reactions to mosquito bites are an increasing clinical concern. due to the lack of availability of mosquito salivary allergens, they are underdiagnosed. here, we reported a newly cloned mosquito aedes (ae.) aegypti salivary allergen.201626608594
the role of octopamine receptor agonists in the synergistic toxicity of certain insect growth regulators (igrs) in controlling dengue vector aedes aegypti (diptera: culicidae) mosquito.the synergistic action of octopamine receptor agonists (or agonists) on many insecticide classes (e.g., organophosphorus, pyrethroids, and neonicotinoids) on aedes aegypti l. has been reported recently. an investigation of or agonist's effect on insect growth regulators (igrs) was undertaken to provide a better understanding of the mechanism of action. based on the igr bioassay, pyriproxyfen was the most potent igr insecticide tested (ec50=0.0019ng/ml). however, the lethal toxicity results indic ...201626672383
off! clip-on repellent device with metofluthrin tested on aedes aegypti (diptera: culicidae) for mortality at different time intervals and distances.the off! clip-on mosquito-repellent device was tested outdoors against aedes aegypti (l.). a single treatment device was used against batches of caged adult, nonblood fed ae. aegypti at multiple locations 0.3m from treatment center. another set of cages was stationed 0.6m from treatment. a final set of cages was placed 0.9m away. trials ran for durations of 5, 15, 30, and 60 min. initial knockdown and mortality after 24 h was recorded. the devices had effective knockdown and mortality. this was ...201626668103
expression profiles and rnai silencing of inhibitor of apoptosis transcripts in aedes, anopheles, and culex mosquitoes (diptera: culicidae).effective mosquito control is vital to curtail the devastating health effects of many vectored diseases. rna interference (rnai)-mediated control of mosquitoes is an attractive alternative to conventional chemical pesticides. previous studies have suggested that transcripts for inhibitors of apoptosis (iaps) may be good rnai targets. to revisit and extend previous reports, we examined the expression of aedes aegypti (l.) iaps (aaeiaps) 1, 2, 5, 6, 9, and a viral iap-associated factor (viaf) as w ...201626659858
spatial clustering of aedes aegypti (diptera: culicidae) and its impact on entomological surveillance indicators.spatial clustering of aedes aegypti (l.) was assessed in 0.25-km2 sampling plots located in three different neighborhoods in the city of rio de janeiro, brazil monitoring was carried out with two oviposition traps (collecting either. eggs or adult mosquitoes) over a consecutive 80-wk period. taylor power laws (tpl) were used to assess clustering in both adult and egg collections. the clustering level observed in both tpl models was sufficient to increase by two to three times the sampling effort ...201626659607
oviposition behavior in aedes aegypti and aedes albopictus (diptera: culicidae) in response to the presence of heterospecific and conspecific larvae.in mosquitoes, location of suitable sites for oviposition requires a set of visual, tactile, and olfactory cues that influences females before laying their eggs. the ability of gravid females to distinguish among potential oviposition sites that will or will not support the growth, development, and survival of their progeny is critical. aedes aegypti (l.) and aedes albopictus (skuse) share ecological niches, being highly competitive in larval stage. we studied the oviposition behavior of both sp ...201626634825
genetic deviation in geographically close populations of the dengue vector aedes aegypti (diptera: culicidae): influence of environmental barriers in south india.mosquitoes are vectors of devastating pathogens and parasites, causing millions of deaths every year. dengue is a mosquito-borne viral infection found in tropical and subtropical regions around the world. recently, dengue transmission has strongly increased in urban and semiurban areas, becoming a major international public health concern. aedes aegypti (diptera: culicidae) is a primary vector of dengue. shedding light on genetic deviation in a. aegypti populations is of crucial importance to fu ...201626627691
hydrothermal synthesis of titanium dioxide nanoparticles: mosquitocidal potential and anticancer activity on human breast cancer cells (mcf-7).mosquito vectors (diptera: culicidae) are responsible for transmission of serious diseases worldwide. mosquito control is being enhanced in many areas, but there are significant challenges, including increasing resistance to insecticides and lack of alternative, cost-effective, and eco-friendly products. to deal with these crucial issues, recent emphasis has been placed on plant materials with mosquitocidal properties. furthermore, cancers figure among the leading causes of morbidity and mortali ...201626621285
sustained efficacy of the novel topical repellent tt-4302 against mosquitoes and ticks.mosquitoes and ticks are blood-feeding pests of humans and animals that can vector many important aetiological agents of disease. previous research demonstrated that tt-4302 (guardian(®) wilderness) containing 5% geraniol applied to human subjects gave 5-6 h of repellency against mosquitoes (depending on species) and was repellent to ticks in vitro. this study was conducted to obtain an independent third-party evaluation of tt-4302 against stegomyia aegypti (= aedes aegypti) (diptera: culicidae) ...201626614394
fitness of walbb wolbachia infection in aedes aegypti: parameter estimates in an outcrossed background and potential for population invasion.wolbachia endosymbionts are potentially useful tools for suppressing disease transmission by aedes aegypti mosquitoes because wolbachia can interfere with the transmission of dengue and other viruses as well as causing deleterious effects on their mosquito hosts. most recent research has focused on the wmel infection, but other infections also influence viral transmission and may spread in natural populations. here, we focus on the walbb infection in an australian outbred background and show tha ...201626711515
larvicidal activity of catechin isolated from leucas aspera against aedes aegypti, anopheles stephensi, and culex quinquefasciatus (diptera: culicidae).vector control is facing a threat due to the emergence of resistance to synthetic insecticides. insecticides of plant origin my serve as an alternative biocontrol technique in the future. the aim of the present study was to evaluate the larvicidal activity of fractions and compounds from the whole-plant methanol extracts of leucas aspera on the fourth-instar larvae of aedes aegypti, anopheles stephensi, and culex quinquefasciatus. the larvae were exposed to fractions with concentrations ranging ...201626711450
[new distribution areas of aedes aegypti in peru]. 201627384639
[suggestions for chikungunya control based on a sensitivity analysis of a mathematical model].chikungunya fever seriously affects peoples' health and causes chronic joint pain and even disability. chikungunya is transmitted by the bite of aedes aegypti and aedes albopictus. outbreaks have been reported in throughout the world, including latin america. mathematical modeling studies of these outbreaks have calculated the values of various​ epidemiological parameters. based on them, a mathematical model was prepared to simulate a chikungunya outbreak in a local population, which was transmi ...201627384636
studies on aedes aegypti resistance to some insecticides in the jazan district, saudi arabia.the present study provided information on the susceptibility status of the adult and larvae of aedes aegypti mosquitoes in jazan region of saudi arabia. bioassay tests were performed on adults and larvae by using who recommended concentrations and test kits. adults of ae. aegypti mosquitoes were exposed to test papers impregnated with lambda-cyhalothrin (0.05%), cyfluthrin (0.15%), deltamethrin (0.05%), permethrin (0.75%), fenitrothion (1%), bendiocarb (0.1%) and ddt (4%) insecticides. ae. aegyp ...201627363057
the eye of the tiger, the thrill of the fight: effective larval and adult control measures against the asian tiger mosquito, aedes albopictus (diptera: culicidae), in north america.the asian tiger mosquito, aedes albopictus (skuse), is a highly invasive container-inhabiting species with a global distribution. this mosquito, similar to other stegomyia species such as aedes aegypti (l.), is highly adapted to urban and suburban areas, and commonly oviposits in artificial containers, which are ubiquitous in these peridomestic environments. the increase in speed and amount of international travel and commerce, coupled with global climate change, have aided in the resurgence and ...201627354440
differentiation of aedes aegypti and aedes notoscriptus (diptera: culicidae) eggs using scanning electron microscopy.aedes notoscriptus and aedes aegypti are both peri-domestic, invasive container-breeding mosquitoes. while the two potential arboviral vectors are bionomically similar, their sympatric distribution in australia is limited. in this study, analyses of ae. aegypti and ae. notoscriptus eggs were enabled using scanning electron microscopy. significant variations in egg length to width ratio and outer chorionic cell field morphology between ae. aegypti and ae. notoscriptus enabled distinction of the t ...201626845557
characterization of enzymatic profiles of aedes aegypti strains from the state of rio grande do norte, brazil.this study was conducted in four strains of aedes aegypti mosquitoes to evaluate the enzymatic activity profiles in the city of mossoró, rio grande do norte, and correlate them with biochemical mechanisms of resistance to insecticides. mosquitos were used to quantify the following detoxification enzymes: mixed-function oxidase (mfo), pnpa-esterase (pnpa-est), and acetylcholinesterase (ache). the profiles were compared statistically with profiles from the rockefeller strain, through the kruskal-w ...201626816185
aedes aegypti (l.) in latin american and caribbean region: with growing evidence for vector adaptation to climate change?within latin america and the caribbean region the impact of climate change has been associated with the effects of rainfall and temperature on seasonal outbreaks of dengue but few studies have been conducted on the impacts of climate on the behaviour and ecology of aedes aegypti mosquitoes.this study was conducted to examine the adaptive behaviours currently being employed by a. aegypti mosquitoes exposed to the force of climate change in lac countries. the literature on the association between ...201626796862
effect of cymbopogon citratus (lemongrass) and syzygium aromaticum (clove) oils on the morphology and mortality of aedes aegypti and anopheles dirus larvae.cymbopogon citratus (lemongrass) and syzygium aromaticum (clove) oils were evaluated to determine mortality rates, morphological aberrations, and persistence when used against third and fourth larval instars of aedes aegypti and anopheles dirus. the oils were evaluated at 1, 5, and 10 % concentrations in mixtures with soybean oil. persistence of higher concentrations was measured over a period of 10 days. for ae. aegypti, both plant oils caused various morphological aberrations to include deform ...201626796022
delivery of chitosan/dsrna nanoparticles for silencing of wing development vestigial (vg) gene in aedes aegypti mosquitoes.rna interference (rnai) has been used as a gene silencing strategy by the introduction of long double stranded rna (dsrna) for the control of pest insects. the aim of the present study was to examine whether the expression of vg gene which is responsible for wing development, can be repressed by chitosan/dsrna based nanoparticles in aedes aegypti. the vestigial gene (vg) was amplified from adult mosquito and cloned in plitmus28i vector. genetically engineered recombinant plasmid was transformed ...201626794313
larvicidal and repellent potential of zingiber nimmonii (j. graham) dalzell (zingiberaceae) essential oil: an eco-friendly tool against malaria, dengue, and lymphatic filariasis mosquito vectors?mosquitoes (diptera: culicidae) are important vectors of terms of public health relevance, especially in tropical and sub-tropical regions. the continuous and indiscriminate use of conventional pesticides for the control of mosquito vectors has resulted in the development of resistance and negative impacts on non-target organisms and the environment. therefore, there is a need for development of effective mosquito control tools. in this study, the larvicidal and repellent activity of zingiber ni ...201626792432
exploiting intimate relationships: controlling mosquito-transmitted disease with wolbachia.mosquito-transmitted diseases impose a growing burden on human health, and current control strategies have proven insufficient to stem the tide. the bacterium wolbachia is a novel and promising form of control for mosquito-transmitted disease. it manipulates host biology, restricts infection with dengue and other pathogens, and alters host reproduction to promote rapid spread in the field. in this review, we examine how the intimate and diverse relationships formed between wolbachia and their mo ...201626776329
calcium influx enhances neuropeptide activation of ecdysteroid hormone production by mosquito ovaries.a critical step in mosquito reproduction is the ingestion of a blood meal from a vertebrate host. in mosquitoes like aedes aegypti, blood feeding stimulates the release of ovary ecdysteroidogenic hormone (oeh) and insulin-like peptide 3 (ilp3). this induces the ovaries to produce ecdysteroid hormone (ecd), which then drives egg maturation. in many immature insects, prothoracicotropic hormone (ptth) stimulates the prothoracic glands to produce ecd that directs molting and metamorphosis. the recep ...201626772671
temporal genetic stability of stegomyia aegypti (= aedes aegypti) populations.the mosquito stegomyia aegypti (= aedes aegypti) (diptera: culicidae) is the primary vector of viruses that cause yellow fever, dengue and chikungunya fever. in the absence of effective vaccines, the reduction of these diseases relies on vector control strategies. the success of these strategies is tightly linked to the population dynamics of target populations. in the present study, 14 collections from st. aegypti populations separated by periods of 1-13 years were analysed to determine their t ...201626744174
detection of aedes aegypti, aedes albopictus, and aedes koreicus in the area of sochi, russia.following the identification of aedes (ae.) aegypti in the sochi area in russia at the beginning of 2000, entomological surveys were conducted during the summers of 2007, 2011, and 2012, leading to the identification of ae. albopictus and ae. koreicus. these findings highlight russia as being the only country in the world health organization european region with a documented presence of both ae. aegypti and ae. albopictus mosquitoes. both mosquito species are found on the coasts of the black sea ...201626741323
bioactivity and laundering resistance of five commercially available, factory-treated permethrin-impregnated fabrics for the prevention of mosquito-borne diseases: the need for a standardized testing and licensing procedure.personal protective measures against hematophagous vectors constitute the first line of defense against arthropod-borne diseases. in this regard, a major advance has been the development of residual insecticides that can be impregnated into clothing. currently, however, information on specific treatment procedures, initial insecticide concentrations, arthropod toxicity, residual activity, and laundering resistance is either fragmentary or non-existent, and no world health organization pesticides ...201626738734
species distribution modelling of aedes aegypti in two dengue-endemic regions of pakistan.statistical tools are effectively used to determine the distribution of mosquitoes and to make ecological inferences about the vector-borne disease dynamics. in this study, we utilised species distribution models to understand spatial patterns of aedes aegypti in two dengue-prevalent regions of pakistan, lahore and swat. species distribution models can potentially indicate the probability of suitability of ae. aegypti once introduced to new regions like swat, where invasion of this species is a ...201626729239
determination of insecticidal effect (lc50 and lc90) of organic fatty acids mixture (c8910+silicone) against aedes aegypti and aedes albopictus (diptera: culicidae).emerging and re-emerging vector-borne diseases such as chikungunya and dengue and associated aedes vectors are expanding their historical ranges; thus, there is a need for the development of novel insecticides for use in vector control programs. the mosquito toxicity of a novel insecticide and repellent consisting of medium-chain carbon fatty acids (c8910) was examined. determination of lc50 and lc90 was made against colony-reared aedes aegypti (l.) and aedes albopictus (skuse) using probit anal ...201626718717
spatial and temporal variation in aedes aegypti and aedes albopictus (diptera: culicidae) numbers in the yogyakarta area of java, indonesia, with implications for wolbachia releases.of mosquito vector populations, particularly through wolbachia endosymbionts. the success of these strategies depends on understanding the dynamics of vector populations. in preparation for wolbachia releases around yogyakarta, we have studied aedes populations in five hamlets. adult monitoring with biogent- sentinel (bg-s) traps indicated that hamlet populations had different dynamics across the year; while there was an increase in aedes aegypti (l.) and aedes albopictus (skuse) numbers in the ...201626576934
identification of a major quantitative trait locus determining resistance to the organophosphate temephos in the dengue vector mosquito aedes aegypti.organophosphate insecticides (op) have extensively been used to control mosquitoes, such as the vector aedes aegypti. unfortunately, op resistance has hampered control programs worldwide. we used quantitative trait locus (qtl) mapping to evaluate temephos resistance in two f1 intercross populations derived from crosses between a resistant ae. aegypti strain (recr) and two susceptible strains (moyod and red). a single major effect qtl was identified on chromosome 2 of both segregating populations ...201626576515
characterization and mosquitocidal potential of neem cake-synthesized silver nanoparticles: genotoxicity and impact on predation efficiency of mosquito natural enemies.mosquitoes (diptera: culicidae) serve as important vectors for a wide number of parasites and pathogens of huge medical and veterinary importance. aedes aegypti is a primary dengue vector in tropical and subtropical urban areas. there is an urgent need to develop eco-friendly mosquitocides. in this study, silver nanoparticles (agnp) were biosynthesized using neem cake, a by-product of the neem oil extraction from the seed kernels of azadirachta indica. agnp were characterized using a variety of ...201626573518
the impact of indoor residual spraying of deltamethrin on dengue vector populations in the peruvian amazon.dengue is an important public health problem in the amazon area of peru, resulting in significant morbidity each year. as in other areas of the world, ultra-low volume (ulv) application of insecticides is the main strategy to reduce adult populations of the dengue vector aedes aegypti, despite growing evidence of its limitations as a single control method. this study investigated the efficacy of deltamethrin s.c. applied through indoor residual spraying (irs) of dwellings in reducing a. aegypti ...201626571068
how important is vertical transmission of dengue viruses by mosquitoes (diptera: culicidae)?vertical transmission of dengue viruses by mosquitoes was discovered at the end of the late 1970s and has been suggested to be a means by which these viruses persist. however, it is unclear how widespread it is in nature, and its importance in the epidemiology of this disease is still debated. here, we review the literature on vertical transmission and discuss its role in dengue's epidemiology and control. we conclude that given the number of studies that failed to find evidence of vertical tran ...201626545718
satyrization and satyrization-resistance in competitive displacements of invasive mosquito species.competitive displacements or reductions of resident populations of insects, often effected by a related species, may be caused by a variety of mechanisms. satyrization is a form of mating interference in which males of one species mate with females of another species, significantly decreasing their fitness and not generating hybrids. satyrization has been established to be the probable cause of competitive displacements of resident mosquitoes by invasive species, especially of aedes aegypti by a ...201626542083
plant-mediated biosynthesis of nanoparticles as an emerging tool against mosquitoes of medical and veterinary importance: a review.mosquitoes (diptera: culicidae) are a key threat for millions of people worldwide, since they act as vectors for devastating parasites and pathogens. mosquito young instars are usually targeted with organophosphates, insect growth regulators and microbial control agents. indoors residual spraying and insecticide-treated bed nets are also employed. however, these chemicals have strong negative effects on human health and the environment. newer and safer tools have been recently implemented to enh ...201626541154
nepenthes ampullaria (nepenthaceae) pitchers are unattractive to gravid aedes aegypti and aedes albopictus (diptera: culicidae).nepenthes pitcher plants are colonized by a variety of specialized arthropods. as aedes mosquitoes are container breeders, nepenthes pitchers are a potential candidate oviposition site for vector species, such as aedes aegypti (l.) and aedes albopictus (skuse). however, aedes spp. are not commonly encountered in nepenthes pitchers, and the environment inside the pitchers of some species is lethal to them. one exception is nepenthes ampullaria jack, whose pitchers are known to be colonized by ae. ...201626518035
improving the effectiveness of three essential oils against aedes aegypti (linn.) and anopheles dirus (peyton and harrison).repellency of essential oil extracted from curcuma longa, eucalyptus globulus, and citrus aurantium at various concentrations (5, 10, 15, 20, and 25 %) with and without 5 % vanillin was evaluated against female mosquitoes: aedes aegypti and anopheles dirus. the comparisons were made with a commercial chemical repellent (n,n-diethyl-3-methylbenzamide (deet) 25 % w/w; kor yor 15) by arm in cage method. it was found that the essential oils with 5 % vanillin gave the longest lasting period against t ...201626358103
the siren's song: exploitation of female flight tones to passively capture male aedes aegypti (diptera: culicidae).the need to capture male mosquitoes has intensified recently as a result of a number of male-based sterile insect technique (sit) and population-modification programs focused on aedes aegypti (l.) having initiated field releases. here, we report the results of the successful exploitation of the attraction of male ae. aegypti to female flight tones to enhance male collections in nonmechanical passive (nonbattery powered) gravid aedes traps (gat). prior to field studies, male attraction to female ...201626502754
the influence of ambient temperature on the susceptibility of aedes aegypti (diptera: culicidae) to the pyrethroid insecticide permethrin.insecticides are the most common strategy used for the management of mosquitoes. changes in ambient temperature can alter the toxicity of insecticides to ectothermic organisms. studies show organophosphate insecticides exhibit a positive correlation between ambient temperature and mortality for many insect species, and carbamate insecticides exhibit a slightly negative correlation between ambient temperature and mortality. pyrethroid insecticides exhibit a distinctly negative correlation between ...201626477050
comparative proteome analysis reveals that blood and sugar meals induce differential protein expression in aedes aegypti female heads.aedes aegypti females ingest sugar or blood to obtain the nutrients needed to maintain cellular homeostasis. during human blood ingestion, female mosquitoes may transmit different viruses such as dengue, yellow fever and, more recently, zika and chikungunya. here, we report changes in protein expression in the heads of adult female ae. aegypti mosquitoes in response to the ingestion of blood or sugar. proteins extracted from the heads of ae. aegypti fed exclusively on blood (bf) or sugar (sf) we ...201627343150
redeployment of a conserved gene regulatory network during aedes aegypti development.changes in gene regulatory networks (grns) underlie the evolution of morphological novelty and developmental system drift. the fruitfly drosophila melanogaster and the dengue and zika vector mosquito aedes aegypti have substantially similar nervous system morphology. nevertheless, they show significant divergence in a set of genes co-expressed in the midline of the drosophila central nervous system, including the master regulator single minded and downstream genes including short gastrulation, s ...201627341759
susceptibility to temephos and spinosad in aedes aegypti (diptera: culicidae) from puerto rico.we examined the susceptibility to temephos and spinosad (natular ec) of eight aedes aegypti (l.) populations from puerto rico, following who method (who 2005). enzyme activity was measured for alpha- and beta-esterases, multiple function oxidases, glutathione-s-transferases, and insensitive acetylcholinesterase and was tested for correlation with the susceptibility level. the results showed that larval populations from puerto rico obtained during 2014 were found to be susceptible to both larvici ...201627330095
structural characterization and evaluation of mosquito-larvicidal property of silver nanoparticles synthesized from the seaweed, turbinaria ornata (turner) j. agardh 1848.the silver nanoparticles synthesized from turbinaria ornata (to-agnps) showed spherical with crystalline nature (20-32 nm) was evaluated against fourth instar larvae of three mosquitoes. the maximum activity of to-agnps was recorded on aedes aegypti followed by anopheles stephensi and culex quinquefasciatus with the following lethal concentration values (μg/ml): lc50 of 0.738, 1.134, and 1.494; and lc90 of 3.342, 17.982, and 22.475, respectively. the obtained respective values (μg/ml) vis-a-vis ...201627327539
temporal frequency of knockdown resistance mutations, f1534c and v1016g, in aedes aegypti in chiang mai city, thailand and the impact of the mutations on the efficiency of thermal fogging spray with pyrethroids.in thailand, control of dengue outbreaks is currently attained by the use of space sprays, particularly thermal fogging using pyrethroids, with the aim of killing infected aedes mosquito vectors in epidemic areas. however, the principal dengue vector, aedes aegypti, is resistant to pyrethroids conferred mainly by mutations in the voltage-gated sodium channel gene, f1534c and v1016g, termed knockdown resistance (kdr). the objectives of this study were to determine the temporal frequencies of f153 ...201627325294
toxicological assessment of spinosad: implications for integrated control of aedes aegypti using larvicides and larvivorous fish.integration of larvivorous fish and biolarvicides at low concentrations to control of mosquito larvae in field situations may result in a safer and more effective tool. however, the usefulness of integrated approach depends upon survival and ecological fitness of fish employed. thus, the aim of this study was to examine the genotoxic effects of combining different sublethal concentrations of spinosad, a naturally occurring neurotoxic insecticide, with male adult poecilid larvivorous guppy (poeci ...201627294296
effects of preexposure to deet on the downstream blood-feeding behaviors of aedes aegypti (diptera: culicidae) mosquitoes.mosquito behavior is heavily influenced by the chemical molecules in the environment. this knowledge can be used to modify insect behaviors; particularly to reduce vector-host contact as a powerful method for disease prevention. n,n-diethyl-meta-toluamide (deet) is the most widely used insect repellent in the market and an excellent example of a chemical that has been used to modify insect behavior for disease prevention. however, genetic insensitivity and habituation in aedes aegypti (l.) mosqu ...201627288690
somatostatin signaling system as an ancestral mechanism: myoregulatory activity of an allatostatin-c peptide in hydra.the coordination of physiological processes requires precise communication between cells. cellular interactions allow cells to be functionally related, facilitating the maintaining of homeostasis. neuropeptides functioning as intercellular signals are widely distributed in metazoa. it is assumed that neuropeptides were the first intercellular transmitters, appearing early during the evolution. in cnidarians, neuropeptides are mainly involved in neurotransmission, acting directly or indirectly on ...201627288244
chemical composition and biological activity of essential oils from different species of piper from panama.the chemical composition of leaf essential oils from 11 species of piper from panama was analyzed by a combination gc-fid and gc-ms procedures. six of them had sesquiterpene hydrocarbons as major constituents, three were characterized by monoterpene hydrocarbons, one by a diterpene, and one by a phenylpropanoid, dillapiole. the main components identified in each species were: cembratrienol (25.4 %) in piper augustum; β-pinene (26.6 %) in piper corrugatum; α-pinene (19.4 %) in piper curtispicum; ...201627286333
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