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zika - an emerging global threat. 201627822403
epidemiological characteristics and risk factors of dengue infection in korean travelers.dengue viral infection has rapidly spread around the world in recent decades. in korea, autochthonous cases of dengue fever have not been confirmed yet. however, imported dengue cases have been increased since 2001. the risk of developing severe dengue in korean has been increased by the accumulation of past-infected persons with residual antibodies to dengue virus and the remarkable growth of traveling to endemic countries in southeast asia. notably, most of imported dengue cases were identifie ...027822922
chikungunya virus infection: an update on joint manifestations and management.the advent of sophisticated diagnostics has enabled the discovery of previously unknown arthropod-borne viruses like chikungunya. this infection has become increasingly prevalent in the last 10 years across the indian ocean and has been brought to media attention by a recent outbreak in the caribbean. the outbreak has been aided by a drastic rise in air travel, allowing infected individuals to transport the virus to previously unaffected regions. in addition, a recently documented viral mutation ...201627824550
predicting wolbachia invasion dynamics in aedes aegypti populations using models of density-dependent demographic traits.arbovirus transmission by the mosquito aedes aegypti can be reduced by the introduction and establishment of the endosymbiotic bacteria wolbachia in wild populations of the vector. wolbachia spreads by increasing the fitness of its hosts relative to uninfected mosquitoes. however, mosquito fitness is also strongly affected by population size through density-dependent competition for limited food resources. we do not understand how this natural variation in fitness affects symbiont spread, which ...201627825343
identification and characterization of micrornas expressed in the african malaria vector anopheles funestus life stages using high throughput sequencing.over the past several years, thousands of micrornas (mirnas) have been identified in the genomes of various insects through cloning and sequencing or even by computational prediction. however, the number of mirnas identified in anopheline species is low and little is known about their role. the mosquito anopheles funestus is one of the dominant malaria vectors in africa, which infects and kills millions of people every year. therefore, small rna molecules isolated from the four life stages (eggs ...201627825380
downregulation of aedes aegypti chromodomain helicase dna binding protein 7/kismet by wolbachia and its effect on dengue virus replication.dengue virus (denv) is a mosquito-transmitted virus imposing a significant burden on human health around the world. since current control strategies are not sufficient, there is an urgent need to find alternative methods to control denv transmission. it has been demonstrated that introduction of wolbachia pipientis in aedes aegypti mosquitoes can impede denv transmission with the mechanism(s) not fully understood. recently, a number of studies have found the involvement of chromodomain dna bindi ...201627827425
larval nutritional stress affects vector life history traits and human malaria transmission.exposure to stress during an insect's larval development can have carry-over effects on adult life history traits and susceptibility to pathogens. we investigated the effects of larval nutritional stress for the first time using field mosquito vectors and malaria parasites. in contrast to previous studies, we show that larval nutritional stress may affect human to mosquito transmission antagonistically: nutritionally deprived larvae showed lower parasite prevalence for only one gametocyte carrie ...201627827429
immunization with brugia malayi myosin as heterologous dna prime protein boost induces protective immunity against b. malayi infection in mastomys coucha.the current control strategies employing chemotherapy with diethylcarbamazine, ivermectin and albendazole have reduced transmission in some filaria-endemic areas, there is growing interest for complementary approaches, such as vaccines especially in light of threat of parasite developing resistance to mainstay drugs. we earlier demonstrated recombinant heavy chain myosin of b. malayi (bm-myo) as a potent vaccine candidate whose efficacy was enhanced by heterologous dna prime/protein boost (myo-p ...201627828973
role of aedes aegypti (linnaeus) and aedes albopictus (skuse) in local dengue epidemics in taiwan.aedes mosquitoes in taiwan mainly comprise aedes albopictus and ae. aegypti. however, the species contributing to autochthonous dengue spread and the extent at which it occurs remain unclear. thus, in this study, we spatially analyzed real data to determine spatial features related to local dengue incidence and mosquito density, particularly that of ae. albopictus and ae. aegypti.201627829399
does fluoride influence oviposition of anopheles stephensi in stored water habitats in an urban setting?the physico-chemical characteristics of lentic aquatic habitats greatly influence mosquito species in selecting suitable oviposition sites; immature development, pupation and adult emergence, therefore are considerations for their preferred ecological niche. correlating water quality parameters with mosquito breeding, as well as immature vector density, are useful for vector control operations in identifying and targeting potential breeding habitats.201627829419
cuba: time to expand health in all policies.the adelaide statement on health in all policies (2010), lays out equity-based principles designed to guide policymakers on incorporating health and well-being components into the development, implementation and evaluation of policy and practice while moving towards shared governance at all levels-local, regional, national and international. special emphasis is placed on cross-sector coordination to achieve policy goals, while improving health and well-being for all.[1] in cuba's case, experienc ...201627829655
zika control through the bacterium wolbachia pipientis. 201627831765
zika virus may affect the universal two-child policy: a new challenge for china. 201627832058
inducing cold-sensitivity in the frigophilic fly drosophila montana by rnai.cold acclimation is a critical physiological adaptation for coping with seasonal cold. by increasing their cold tolerance individuals can remain active for longer at the onset of winter and can recover more quickly from a cold shock. in insects, despite many physiological studies, little is known about the genetic basis of cold acclimation. recently, transcriptomic analyses in drosophila virilis and d. montana revealed candidate genes for cold acclimation by identifying genes upregulated during ...201627832122
temporal and spatial evolution of dengue incidence in brazil, 2001-2012.in brazil, the incidence of dengue greatly increased in the last two decades and there are several factors impeding the control of the disease. the present study focused on describing the space-time evolution of dengue in brazil from 2001 to 2012 and analyzing the relationship of the reported cases with socio-demographic and environmental factors.201627832129
thoughts around the zika virus crisis.as a lot of reviews are available on zika virus (zikv), in this short commentary, we will focus on the recent advances and gaps in knowledge regarding our understanding of zikv infections and on the reaction to the "zikv crisis."027832453
green synthesis of selenium nanoparticles conjugated clausena dentata plant leaf extract and their insecticidal potential against mosquito vectors.mosquitoes are major vectors for the transmission of many diseases like chikungunya, malaria, dengue, zika, etc. worldwide. in the present study, selenium nanoparticles (senps) were synthesized from clausena dentata and were tested for their larvicidal efficacy against the fourth-instar larvae of anopheles stephensi, aedes aegypti, and culex quinquefasciatus. the synthesized nanoparticles were characterized using uv-vis spectroscopy, fourier transform infrared radiation (ftir) spectroscopy, edax ...201627832715
effects of periplocoside p from periploca sepium on the midgut transmembrane potential of mythimna separata larvae.periplocoside p (psp) isolated from the root bark of periploca sepium contains a pregnane glycoside skeleton and possesses high insecticidal properties. preliminary studies indicated that psp disrupts epithelial functions in the midgut of lepidopteran larvae. in the present study, we examined the effects of psp on the apical and basolateral membrane voltages, va and vbl, respectively, of cells from (1) midguts isolated from the larvae of the oriental armyworm mythimna separata that were in vitro ...201627833169
host plant affects morphometric variation of diaphorina citri (hemiptera: liviidae).the asian citrus psyllid (acp), diaphorina citri kuwayama, is one of the most serious citrus pests worldwide due to its role as vector of huanglongbing or citrus greening disease. while some optimal plant species for acp oviposition and development have been identified, little is known of the influence of host plants on acp size and shape. our goal was to determine how size and shape of acp wing and body size varies when development occurs on different host plants in a controlled rearing environ ...201627833820
plant virus-insect vector interactions: current and potential future research directions.acquisition and transmission by an insect vector is central to the infection cycle of the majority of plant pathogenic viruses. plant viruses can interact with their insect host in a variety of ways including both non-persistent and circulative transmission; in some cases, the latter involves virus replication in cells of the insect host. replicating viruses can also elicit both innate and specific defense responses in the insect host. a consistent feature is that the interaction of the virus wi ...201627834855
zika virus epidemic in brazil. i. fatal disease in adults: clinical and laboratorial aspects.zika virus (zikv) was first detected in brazil in may 2015 and the country experienced an explosive epidemic. however, recent studies indicate that the introduction of zikv occurred in late 2013. cases of microcephaly and deaths associated with zikv infection were identified in brazil in november, 2015.201627835759
dynamic expression of genes encoding subunits of inward rectifier potassium (kir) channels in the yellow fever mosquito aedes aegypti.inward rectifier potassium (kir) channels play fundamental roles in neuromuscular, epithelial, and endocrine function in mammals. recent research in insects suggests that kir channels play critical roles in the development, immune function, and excretory physiology of fruit flies and/or mosquitoes. moreover, our group has demonstrated that mosquito kir channels may serve as valuable targets for the development of novel insecticides. here we characterize the molecular expression of 5 mrnas encodi ...201727836744
distinguishing between zika and spondweni viruses. 027843157
reining in angola's yellow fever outbreak.rosa moreira tells andréia azevedo soares how angola reined in its worst yellow fever epidemic in 30 years.027843160
use of transgenic aedes aegypti in brazil: risk perception and assessment.the ox513a strain of aedes aegypti, which was developed by the british company oxitec, expresses a self-limiting transgene that prevents larvae from developing to adulthood. in april 2014, the brazilian national technical commission on biosafety completed a risk assessment of ox513a and concluded that the strain did not present new biological risks to humans or the environment and could be released in brazil. at that point, brazil became the first country to approve the unconstrained release of ...201627843167
wolbachia: the selfish trojan horse in dengue control.dengue fever has re-emerged as a major public health challenge. of late, several promising attempts have been made to control the disease with limited success. an innovative method of biological control of dengue is the use of the bacterium wolbachia. selected strains of wolbachia have been introduced into aedes aegypti to prevent transmission of dengue viruses by the vector. wolbachia prevents dengue transmission by either directly blocking the virus or by decreasing the lifespan of the vector. ...201627843186
anterior ischemic optic neuropathy following dengue fever.dengue fever is caused by a flavivirus. this infection is endemic in the tropics and warm temperate regions of the world. ocular manifestations of dengue fever include subconjunctival, vitreous, and retinal haemorrhages; posterior uveitis; optic neuritis; and maculopathies, haemorrhage, and oedema. however anterior ischemic optic neuropathy is a rare presentation. optic nerve ischemia most frequently occurs at the optic nerve head, where structural crowding of nerve fibers and reduction of the v ...027843231
trends in clinical trials of dengue vaccine.dengue is one of the most important vector-borne disease and an increasing problem worldwide because of current globalization trends. roughly, half the world's population lives in dengue endemic countries, and nearly 100 million people are infected annually with dengue. india has the highest burden of the disease with 34% of the global cases. in the context of an expanding and potentially fatal infectious disease without effective prevention or specific treatment, the public health value of a pr ...027843790
repellency and larvicidal activity of essential oils from xylopia laevigata, xylopia frutescens, lippia pedunculosa, and their individual compounds against aedes aegypti linnaeus.in order to find new alternatives for vector control and personal protection, we evaluated the larvicidal and repellent activity of essentials oils from plants found in the northeast of brazil against aedes aegypti linnaeus mosquitoes. the plants tested include xylopia laevigata, xylopia frutescens, and lippia pedunculosa and their major compounds, piperitenone oxide, and (r)-limonene. the essential oil of l. pedunculosa and its major volatile compounds were shown to be toxic for ae. aegypti lar ...201727844468
hiv and zika: when will we be able to end these epidemics? 201627846847
evolutionarily conserved odorant receptor function questions ecological context of octenol role in mosquitoes.olfaction is a key insect adaptation to a wide range of habitats. in the last thirty years, the detection of octenol by blood-feeding insects has been primarily understood in the context of animal host-seeking. the recent discovery of a conserved octenol receptor gene in the strictly nectar-feeding elephant mosquito toxorhynchites amboinensis (taor8) suggests a different biological role. here, we show that taor8 is a functional ortholog of its counterparts in blood-feeding mosquitoes displaying ...201627849027
review of west nile virus circulation and outbreak risk in madagascar: entomological and ornithological perspectives.west nile fever (wnf) is a zoonotic disease, occurring nearly globally. in madagascar, west nile virus (wnv) was first detected in 1978 from wild birds and the virus is currently distributed across the island, but no epidemic or epizootic period has been recorded. one fatal human case of wnv infection was reported in 2011, suggesting a "tip of the iceberg" phenomenon of a possible wnf epidemic/epizootic on the island. the main objective of this literature-based survey is to review patterns of wn ...201627849515
the genome of the crustacean parhyale hawaiensis, a model for animal development, regeneration, immunity and lignocellulose digestion.the amphipod crustacean parhyale hawaiensis is a blossoming model system for studies of developmental mechanisms and more recently regeneration. we have sequenced the genome allowing annotation of all key signaling pathways, transcription factors, and non-coding rnas that will enhance ongoing functional studies. parhyale is a member of the malacostraca clade, which includes crustacean food crop species. we analysed the immunity related genes of parhyale as an important comparative system for the ...201627849518
yellow fever virus capsid protein is a potent suppressor of rna silencing that binds double-stranded rna.mosquito-borne flaviviruses, including yellow fever virus (yfv), zika virus (zikv), and west nile virus (wnv), profoundly affect human health. the successful transmission of these viruses to a human host depends on the pathogen's ability to overcome a potentially sterilizing immune response in the vector mosquito. similar to other invertebrate animals and plants, the mosquito's rna silencing pathway comprises its primary antiviral defense. although a diverse range of plant and insect viruses has ...201627849599
zika mosquito vectors: the jury is still out.after a 40-year hiatus, the international congress of entomology (ice 2016) convened in orlando, florida (september 25-30, 2016). one of the symposia at ice 2016, the zika symposium, covered multiple aspects of the zika epidemic, including epidemiology, sexual transmission, genetic tools for reducing transmission, and particularly vector competence. while there was a consensus among participants that the yellow fever mosquito, aedes aegypti, is a vector of the zika virus, there is growing eviden ...201627853521
screening of fungi for biological control of a triatomine vector of chagas disease: temperature and trypanosome infection as factors.entomopathogenic fungi have been investigated as an alternative tool for controlling various insects, including triatomine vectors of the protozoan trypanosoma cruzi, the etiological agent of chagas disease. here we tested the pathogenicity and virulence of ten isolates of the fungi metarhizium spp. and beauveria bassiana against rhodnius prolixus and found all of the isolates to be virulent. we used two isolates (urpe-11 metarhizium anisopliae and ent-1 beauveria bassiana) for further screening ...201627855217
wolbachia-based dengue virus inhibition is not tissue-specific in aedes aegypti.dengue fever, caused by the dengue virus (denv), is now the most common arbovirus transmitted disease globally. one novel approach to control denv is to use the endosymbiotic bacterium, wolbachia pipientis, to limit denv replication inside the primary mosquito vector, aedes aegypti. wolbachia that is naturally present in a range of insects reduces the capacity for viruses, bacteria, parasites and fungi to replicate inside insects. wolbachia's mode of action is not well understood but may involve ...201627855218
febrile illness diagnostics and the malaria-industrial complex: a socio-environmental perspective.global prioritization of single-disease eradication programs over improvements to basic diagnostic capacity in the global south have left the world unprepared for epidemics of chikungunya, ebola, zika, and whatever lies on the horizon. the medical establishment is slowly realizing that in many parts of sub-saharan africa (ssa), particularly urban areas, up to a third of patients suffering from acute fever do not receive a correct diagnosis of their infection.201627855644
perinatal chikungunya in twins.we report a case of vertically transmitted chikungunya infection in heterozygous twin neonates presenting as seizures, encephalopathy, midfacial hyperpigmentation, anemia, and thrombocytopenia. this could be considered as a rare cause of neonatal seizure and identification would help in appropriate management.027857791
in vitro effect of seven essential oils on the reproduction of the cattle tick rhipicephalus microplus.the acaricidal effect of seven essential oils was examined in vitro against the cattle tick (rhipicephalus microplus). engorged female ticks were manually collected in farms of southern brazil and placed into petri dishes (n = 10) in order to test the following oils: juniper (juniperus communis), palmarosa (cymbopogon martinii), cedar (cedrus atlantica), lemon grass (cymbopogon citratus), ginger (zingiber officinale), geranium (pelargonium graveolens) and bergamot (citrus aurantium var bergamia) ...201627857849
evaluation of collection methods for culex quinquefasciatus, aedes aegypti, and aedes simpsoni in northeastern tanzania.in east africa, significant morbidity and mortality are caused by infections spread by culex quinquefasciatus and aedes aegypti. sticky traps have been shown to be effective tools for sampling populations of aedes mosquitoes and have been found to catch cx. quinquefasciatus. thus, they could potentially be used to sample populations of this species. this study compared sticky ovitraps (so) and mosquitraps (mqt) with the cdc gravid trap (cdc-gt) for collection of culex and aedes mosquito populati ...201627860009
movement of aedes aegypti following a sugar meal and its implication in the development of control strategies in durán, ecuador.we evaluated how the presence of sugar sources impacted the distribution of aedes aegypti in different habitats in durán, ecuador. land cover and normalized difference vegetation index maps were used to guide a random point sampling routine to select study grids (30 m × 30 m) in low vegetation (lv) and high vegetation (hv). five individual plants, at one home in the lv and hv grid, were treated with a different colored, non-attractive, 60% sucrose solution to determine mosquito feeding and movem ...201627860016
the driving force for 2014 dengue outbreak in guangdong, china.dengue fever has rapidly spread in recent decades to become the most globally expansive viral vector-borne disease. in mainland china, a number of dengue outbreaks have been reported since 1978, but the worst epidemic in decades, involving 45230 cases and 76 imported cases, resulting in six deaths in guangdong province, emerged in 2014. reasons for this ongoing surge in dengue, both imported and autochthonous, are currently unclear and demand urgent investigation. here, a seasonally-driven dynam ...201627861514
phylosymbiosis: relationships and functional effects of microbial communities across host evolutionary history.phylosymbiosis was recently proposed to describe the eco-evolutionary pattern, whereby the ecological relatedness of host-associated microbial communities parallels the phylogeny of related host species. here, we test the prevalence of phylosymbiosis and its functional significance under highly controlled conditions by characterizing the microbiota of 24 animal species from four different groups (peromyscus deer mice, drosophila flies, mosquitoes, and nasonia wasps), and we reevaluate the phylos ...201627861590
epidemiological characteristics of yellow fever in brazil, 2000-2012.this study aims to describe the epidemiological characteristics of yellow fever in brazil in the period 2000-2012.201627861674
dibenzo-α-pyrones: a new class of larvicidal metabolites against aedes aegypti from the endophytic fungus hyalodendriella sp. ponipodef12.in our search for new agrochemicals from endophytic fungi, the crude extract of the endophytic hyalodendriella sp. ponipodef12 associated with the hybrid 'neva' of populus deltoides marsh × p. nigra l. was found to possess larvicidal activity against aedes aegypti.201627862895
understanding zika virus.this article describes what pediatric healthcare professionals should know about zika virus (zikv).201727862959
analyses of density-dependent effects are needed to understand how and when wolbachia can control dengue vectors.releases of wolbachia-infected mosquitoes have been shown to be an effective method of controlling aedes aegypti, the main vector of dengue fever, in australia. a study in bmc biology from penelope hancock and others shows that incorporation of density-dependent effects into population models can provide major improvements in understanding how and when the infected populations can become established.see research article: https://bmcbiol.biomedcentral.com/articles/10.1186/s12915-016-0319-5 .201627863487
assessment of icount software, a precise and fast egg counting tool for the mosquito vector aedes aegypti.widespread in the tropics, the mosquito aedes aegypti is an important vector of many viruses, posing a significant threat to human health. vector monitoring often requires fecundity estimation by counting eggs laid by female mosquitoes. traditionally, manual data analyses have been used but this requires a lot of effort and is the methods are prone to errors. an easy tool to assess the number of eggs laid would facilitate experimentation and vector control operations.201627863526
establishment of aedes aegypti (l.) in mountainous regions in mexico: increasing number of population at risk of mosquito-borne disease and future climate conditions.the study was conducted in the central region of veracruz mexico, in the metropolitan area of xalapa. it is a mountainous area where aedes aegypti (l.) is not currently endemic. an entomological survey was done along an elevation gradient using the ae. aegypti occurrences at different life cycle stages. seven sites were sampled and a total of 24 mosquito species were recorded: 9 species were found in urban areas, 18 in non-urban areas with remnant vegetation, and 3 occurred in both environments. ...201727863974
sarniensine, a mesembrine-type alkaloid isolated from nerine sarniensis, an indigenous south african amaryllidaceae, with larvicidal and adulticidal activities against aedes aegypti.a new mesembrine-type alkaloid, named sarniensine, was isolated together with tazettine, lycorine, the main alkaloid, and 3-epimacronine from nerine sarniensis, with the last two produced for the first time by this plant. this amaryllidaceae, which is indigenous of south africa, was investigated for its alkaloid content, because the organic extract of its bulbs showed strong larvicidal activity with an lc50 value of 0.008μgμl(-1) against first instar aedes aegypti larvae and with an ld50 value 4 ...201727864138
housing improvement: a novel paradigm for urban vector-borne disease control? 201627864518
the dna chaperone hmgb1 potentiates the transcriptional activity of rel1a in the mosquito aedes aegypti.high mobility group protein 1 (hmgb1) is a non-histone, chromatin-associated nuclear protein that functions in regulating eukaryotic gene expression. we investigated the influence and mechanism of action of aedes aegypti hmgb1 (aahmgb1) on mosquito rel1a-mediated transcription from target gene promoters. the dna-binding domain (rhd) of aarel1a was bacterially expressed and purified, and aahmgb1 dramatically enhanced rhd binding to consensus nf-kb/rel dna response elements. luciferase reporter an ...201727867076
essential oil composition, antimicrobial and pharmacological activities of lippia sidoides cham. (verbenaceae) from são gonçalo do abaeté, minas gerais, brazil.lippia sidoides (verbenaceae) is used in brazilian folk medicine as an antiseptic, and it is usually applied topically on skin, mucous membranes, mouth, and throat, or used for vaginal washings.027867267
factors influencing infection and transmission of anopheles gambiae densovirus (agdnv) in mosquitoes.anopheles gambiae densovirus (agdnv) is a potential microbial agent for paratransgenesis and gene transduction in an. gambiae, the major vector of human malaria in sub-saharan africa. understanding the interaction between agdnv and an. gambiae is critical for using agdnv in a basic and applied manner for anopheles gene manipulation. here, we tested the effects of mosquito age, sex, blood feeding status, and potential for horizontal transmission using an enhanced green fluorescent protein (egfp) ...201627867767
[first report of the f1534c mutation associated with cross-resistance to ddt and pyrethroids in aedes aegypti from colombia].the main strategy for the control of aedes aegypti, vector of dengue, chikungunya and zika viruses, is based on the use of insecticides to reduce its populations. however, their use has led to insect resistance to these chemicals. objective: to determine the presence of the f1534c mutation associated with cross-resistance to ddt and pyrethroids in a. aegypti in sincelejo, colombia. materials and methods: we studied nine specimens of a. aegypti that showed resistance to lambdacyhalothrin in bioas ...201627869391
[recommendations for the surveillance of aedes aegypti].diseases caused by arboviruses transmitted by aedes aegypti, such as dengue, chikungunya and zika, continue to rise in annual incidence and geographic expansion. a key limitation for achieving control of a. aegypti has been the lack of effective tools for monitoring its population, and thus determine what control measures actually work. surveillance of a. aegypti has been based mainly on immature indexes, but they bear little relation to the number of mosquito females, which are the ones capable ...201627869394
assessing the epidemic potential of rna and dna viruses.many new and emerging rna and dna viruses are zoonotic or have zoonotic origins in an animal reservoir that is usually mammalian and sometimes avian. not all zoonotic viruses are transmissible (directly or by an arthropod vector) between human hosts. virus genome sequence data provide the best evidence of transmission. of human transmissible virus, 37 species have so far been restricted to self-limiting outbreaks. these viruses are priorities for surveillance because relatively minor changes in ...027869592
a genome-wide identification and analysis of the basic helix-loop-helix transcription factors in brown planthopper, nilaparvata lugens.the basic helix-loop-helix (bhlh) transcription factors in insects play essential roles in multiple developmental processes including neurogenesis, sterol metabolism, circadian rhythms, organogenesis and formation of olfactory sensory neurons. the identification and function analysis of bhlh family members of the most destructive insect pest of rice, nilaparvata lugens, may provide novel tools for pest management. here, a genome-wide survey for bhlh sequences identified 60 bhlh sequences (nlbhlh ...201627869716
mosquito oviposition behavior and vector control.the burden of gene transfer from one mosquito generation to the next falls on the female and her eggs. the selection of an oviposition site that guarantees egg and larval survival is a critical step in the reproductive process. the dangers associated with ephemeral aquatic habitats, lengthy droughts, freezing winters, and the absence of larval nutrition makes careful oviposition site selection by a female mosquito extremely important. mosquito species exhibit a remarkable diversity of ovipositio ...201627869724
no magic bullet: citizenship and social participation in the control of aedes aegypti. 201627869933
[aedes aegypti control strategies: a review].to describe the main strategies to control aedes aegypti, with emphasis on promising technological innovations for use in brazil.201627869956
incidence of dengue cases (2007-2013) and seasonal distribution of mosquitoes (diptera: culicidae) (2012-2013) in barreiras, bahia, brazil.to describe seasonal mosquito distribution and the incidence of dengue cases in barreiras, bahia, brazil.201627869967
methods and procedures used in aedes aegypti control in the successful campaign for yellow fever prophylaxis in rio de janeiro, brazil, in 1928 and 1929.to review the challenges and procedures used in aedes aegypti control and the characteristics of the epidemics in the successful campaign, led by clementino fraga, for yellow fever control in rio de janeiro, brazil, in 1928 and 1929, before the vaccine (1937) and ddt (1947).201627869977
identification of genes for engineering the male germline of aedes aegypti and ceratitis capitata.synthetic biology approaches are promising new strategies for control of pest insects that transmit disease and cause agricultural damage. these strategies require characterised modular components that can direct appropriate expression of effector sequences, with components conserved across species being particularly useful. the goal of this study was to identify genes from which new potential components could be derived for manipulation of the male germline in two major pest species, the mosqui ...201627871244
dengue with a morbilliform rash and a positive tourniquet test. 201627872891
low circulation of zika virus, cambodia, 2007-2016.we describe a retrospective study on circulation of zika virus in cambodia during 2007-2016 among patients with dengue-like symptoms and aedes aegypti mosquitoes. our findings suggest that zika virus in cambodia belongs to the asia genotype, is endemic, has low prevalence, and has had low-level impact on public health.201727875110
generation of monoclonal antibodies against native viral proteins using antigen-expressing mammalian cells for mouse immunization.due to their rising incidence and progressive geographical spread, infections with mosquito-borne viruses, such as dengue (denv), chikungunya and zika virus, have developed into major public health challenges. since all of these viruses may cause similar symptoms and can occur in concurrent epidemics, tools for their differential diagnosis and epidemiological monitoring are of urgent need.201627876044
methanolic extract of agerantum conyzoides exhibited toxicity and growth disruption activities against anopheles gambiae sensu stricto and anopheles arabiensis larvae.vector control remains the mainstay to effective malaria management. the negative implications following persistent application of synthetic insecticides geared towards regulation of mosquito populations have necessitated prospection for ecofriendly effective chemistries. plant-derived compounds have the potential to control malaria-transmitting mosquito populations. previously, agerantum conyzoides extracts have demonstrated toxicity effects on disease-transmitting mosquitoes. however, their ef ...201627876055
long-term spatio-temporal dynamics of the mosquito aedes aegypti in temperate argentina.buenos aires city is located near the southern limit of the distribution of aedes aegypti (diptera: culicidae). this study aimed to assess long-term variations in the abundance of ae. aegypti in buenos aires in relation to changes in climatic conditions. ae. aegypti weekly oviposition activity was analyzed and compared through nine warm seasons from 1998 to 2014, with 200 ovitraps placed across the whole extension of the city. the temporal and spatial dynamics of abundances were compared among s ...201727876100
conserved chemosensory proteins in the proboscis and eyes of lepidoptera.odorant-binding proteins (obps) and chemosensory proteins (csps) are endowed with several different functions besides being carriers for pheromones and odorants. based on a previous report of a csp acting as surfactant in the proboscis of the moth helicoverpa armigera, we revealed the presence of orthologue proteins in two other moths plutella xylostella and chilo suppressalis, as well as two butterflies papilio machaon and pieris rapae, using immunodetection and proteomic analysis. the unusual ...201627877091
neurological manifestations of zika virus infection.zika virus (zikv) is a flavivirus (flaviviridae family) transmitted mainly by aedes mosquitoes. the virus was restricted to the african continent until its spread to south-east asia in the 1980's, the micronesia in 2007, the french polynesia in 2013 and, more recently in the americas in 2015, where, up to date, the world health organization (who) has estimated about 3-4 million total cases of zikv infection. during outbreaks in the french polynesia and brazil in 2013 and 2015, respectively, nati ...027878100
susceptibility profile and metabolic mechanisms involved in aedes aegypti and aedes albopictus resistant to ddt and deltamethrin in the central african republic.aedes aegypti and ae. albopictus are the main epidemic vectors of dengue, chikungunya and zika viruses worldwide. their control during epidemics relies mainly on control of larvae and adults with insecticides. unfortunately, loss of susceptibility of both species to several insecticide classes limits the efficacy of interventions. in africa, where aedes-borne viruses are of growing concern, few data are available on resistance to insecticides. to fill this gap, we assessed the susceptibility to ...201627881148
efficacy of ulv and thermal aerosols of deltamethrin for control of aedes albopictus in nice, france.ultra-low volume (ulv) insecticidal aerosols dispensed from vehicle-mounted cold-foggers are widely considered the method of choice for control of aedes aegypti and ae. albopictus during outbreaks of dengue and chikungunya and, more recently, zika. nevertheless, their effectiveness has been poorly studied, particularly in europe. nearly all published studies of ulv efficacy are bio-assays based on the mortality of caged mosquitoes. in our study we preferred to monitor the direct impact of treatm ...201627881181
prolonged shedding of chikungunya virus in semen and urine: a new perspective for diagnosis and implications for transmission.we report the presence of chikungunya (chikv) rna in the blood, urine and semen during the acute phase of the disease in an adult with a dual infection with dengue virus type 3. the patient, a 25 yr-old man from salvador, brazil, reported a 6-day duration of high fever, arthralgia, myalgia, headache and photophobia.blood and semen specimens were positive for chikv in the first collected samples; semen and urine specimens were positive for chikv after 30 days of symptoms onset. denv-3 rna was pos ...201627882301
proteomic profile of the bradysia odoriphaga in response to the microbial secondary metabolite benzothiazole.benzothiazole, a microbial secondary metabolite, has been demonstrated to possess fumigant activity against sclerotinia sclerotiorum, ditylenchus destructor and bradysia odoriphaga. however, to facilitate the development of novel microbial pesticides, the mode of action of benzothiazole needs to be elucidated. here, we employed itraq-based quantitative proteomics analysis to investigate the effects of benzothiazole on the proteomic expression of b. odoriphaga. in response to benzothiazole, 92 of ...201627883048
tsetse fly tolerance to t. brucei infection: transcriptome analysis of trypanosome-associated changes in the tsetse fly salivary gland.for their transmission, african trypanosomes rely on their blood feeding insect vector, the tsetse fly (glossina sp.). the ingested trypanosoma brucei parasites have to overcome a series of barriers in the tsetse fly alimentary tract to finally develop into the infective metacyclic forms in the salivary glands that are transmitted to a mammalian host by the tsetse bite. the parasite population in the salivary gland is dense with a significant number of trypanosomes tightly attached to the epithe ...201627884110
aedes aegypti rhesus glycoproteins contribute to ammonia excretion by larval anal papillae.in larval aedes aegypti, transcripts of the rhesus-like glycoproteins aerh50-1 and aerh50-2 have been detected in the anal papillae, sites of ammonia (nh3/nh4(+)) excretion; however, these putative ammonia transporters have not been previously localized or functionally characterized. in this study, we show that the aerh50s co-immunolocalize with apical v-type h(+)-atpase as well as with basal na(+)/k(+)-atpase in the epithelium of anal papillae. the double-stranded rna-mediated knockdown of aerh ...201727885043
frequency of the val1016ile mutation on the kdr gene in aedes aegypti (diptera: culicidae) in south brazil.recently, the number of aedes aegypti foci has increased in west of santa catarina, south brazil, which has increased concern regarding mosquito-borne disease outbreaks such as dengue fever, zika virus, and chikungunya. therefore, it is important to monitor genetic resistance to insecticides through "knockdown resistance". homozygosity (ile/ile) at position 1016 in the coding region of a voltage-dependent sodium channel gene (nav) may induce resistance to pyrethroid insecticides. we evaluated th ...201627886338
a prospective cohort study to assess seroprevalence, incidence, knowledge, attitudes and practices, willingness to pay for vaccine and related risk factors in dengue in a high incidence setting.dengue is one of the most important vector-borne diseases in the world, causing significant morbidity and economic impact. in colombia, dengue is a major public health problem. departments of la guajira, cesar and magdalena are dengue endemic areas. the objective of this research is to determine the seroprevalence and the incidence of dengue virus infection in the participating municipalities from these departments, and also establish the association between individual and housing factors and ve ...201627887591
editorial brain malformation surveillance in the zika era.the current surveillance systems for congenital microcephaly are necessary to monitor the impact of zika virus (zikv) on the developing human brain, as well as the zikv prevention efforts. however, these congenital microcephaly surveillance systems are insufficient. abnormalities of neuronal differentiation, development and migration may occur among infants with normal head circumference who have intrauterine exposure to zikv. therefore, surveillance for congenital microcephaly does not ascertai ...027891785
fern-synthesized silver nanocrystals: towards a new class of mosquito oviposition deterrents?mosquitoes act as vectors of devastating pathogens and parasites, representing a key threat for millions of humans and animals worldwide. eco-friendly control tools are urgently required. we proposed a novel method of fern-mediated biosynthesis of silver nanoparticles (agnp) using dicranopteris linearis, acting as a reducing and capping agent. agnp were characterized by uv-vis spectroscopy, fourier transform infrared (ftir) spectroscopy, scanning electron microscopy (sem), energy-dispersive x-ra ...201627892872
zika virus-what the otolaryngologist should know: a review.initially discovered in 1947, zika virus infection received little notoriety as a tropical disease until 2015 when an outbreak of microcephaly cases was reported in brazil. zika is a single-stranded rna arbovirus of the flaviviridae family. the primary source of infection in humans stems from aedes aegypti mosquito bites but can also occur through sexual, blood, and perinatal transmission. with expectations that 3 to 4 million people across the americas will be infected over the next year, the w ...201727892990
diet-induced nutritional stress and pathogen interference in wolbachia-infected aedes aegypti.the pathogen interference phenotype greatly restricts infection with dengue virus (denv) and other pathogens in wolbachia-infected aedes aegypti, and is a vital component of wolbachia-based mosquito control. critically, the phenotype's causal mechanism is complex and poorly understood, with recent evidence suggesting that the cause may be species specific. to better understand this important phenotype, we investigated the role of diet-induced nutritional stress on interference against denv and t ...201627893736
analysis of synonymous codon usage bias of zika virus and its adaption to the hosts.zika virus (zikv) is a mosquito-borne virus (arbovirus) in the family flaviviridae, and the symptoms caused by zikv infection in humans include rash, fever, arthralgia, myalgia, asthenia and conjunctivitis. codon usage bias analysis can reveal much about the molecular evolution and host adaption of zikv. to gain insight into the evolutionary characteristics of zikv, we performed a comprehensive analysis on the codon usage pattern in 46 zikv strains by calculating the effective number of codons ( ...201627893824
successful malaria elimination in the ecuador-peru border region: epidemiology and lessons learned.in recent years, malaria (plasmodium vivax and plasmodium falciparum) has been successfully controlled in the ecuador-peru coastal border region. the aim of this study was to document this control effort and to identify the best practices and lessons learned that are applicable to malaria control and to other vector-borne diseases. a proximal outcome evaluation was conducted of the robust elimination programme in el oro province, ecuador, and the tumbes region, peru. data collection efforts incl ...201627894320
analysis of chikungunya virus proteins reveals that non-structural proteins nsp2 and nsp3 exhibit rna interference (rnai) suppressor activity.rnai pathway is an antiviral defence mechanism employed by insects that result in degradation of viral rna thereby curbing infection. several viruses including flaviviruses encode viral suppressors of rnai (vsrs) to counteract the antiviral rnai pathway. till date, no vsr has been reported in alphaviruses. the present study was undertaken to evaluate chikungunya virus (chikv) proteins for rnai suppressor activity. we systematically analyzed all nine chikv proteins for rnai suppressor activity us ...201627901124
wolbachia suppresses cell fusing agent virus in mosquito cells.the genus flavivirus contains a large number of positive-sense ssrna viruses. while some are transmitted by mosquitoes or other arthropods and are pathogenic to humans and animals (e.g. dengue and zika viruses), some are insect-specific and do not replicate in vertebrate cells. these are known as insect-specific flaviviruses (isfs). cell fusing agent virus (cfav) was the first described isf, which was detected in an aedes aegypti cell line, aag2. here, we investigated the effect of wolbachia, a ...201627902358
spatial pattern evolution of aedes aegypti breeding sites in an argentinean city without a dengue vector control programme.the main objective of this study was to obtain and analyse the space-time dynamics of aedes aegypti breeding sites in clorinda city, formosa province, argentina coupled with landscape analysis using the maximum entropy approach in order to generate a dengue vector niche model. in urban areas, without vector control activities, 12 entomologic (larval) samplings were performed during three years (october 2011 to october 2014). the entomologic surveillance area represented 16,511 houses. predictive ...201627903056
sequence diversity of dengue virus type 2 in brain and thymus of infected interferon receptor ko mice: implications for dengue virulence.we previously reported that a clinical isolate of dengue virus (denv) is capable of causing acute-phase systemic infection in mice harboring knockouts of the genes encoding type-i and -ii interferon ifn receptors (ifn-α/β/γr ko mice); in contrast, other virulent denv isolates exhibited slow disease progression in this mice, yielding lethal infection around 20 days post-infection (p.i.). in the present study, we sought to clarify the dynamics of slow disease progression by examining disease progr ...201627903277
[reasons to recommend vaccination against dengue in easter island: immunization advisory committee of sociedad chilena de infectología].dengue was first diagnosed on easter island on year 2002 and thereafter recurrent outbreaks have occurred involving different serotypes of dengue virus. its vector, aedes aegypti has not been eliminated despite the small size of the island. conditions at the local hospital preclude adequate management of severe and hemorrhagic cases due to the absence of a critical care unit as well as no availability of platelets, or plasma units for transfusion. besides, transfer, of severely affected patients ...201627905629
comparative genomics of beauveria bassiana: uncovering signatures of virulence against mosquitoes.entomopathogenic fungi such as beauveria bassiana are promising biological agents for control of malaria mosquitoes. indeed, infection with b. bassiana reduces the lifespan of mosquitoes in the laboratory and in the field. natural isolates of b. bassiana show up to 10-fold differences in virulence between the most and the least virulent isolate. in this study, we sequenced the genomes of five isolates representing the extremes of low/high virulence and three rna libraries, and applied a genome c ...201627905873
size, sounds and sex: interactions between body size and harmonic convergence signals determine mating success in aedes aegypti.several new mosquito control strategies will involve the release of laboratory reared males which will be required to compete with wild males for mates. currently, the determinants of male mating success remain unclear. the presence of convergence between male and female harmonic flight tone frequencies during a mating attempt have been found to increase male mating success in the yellow fever mosquito, aedes aegypti. size has also been implicated as a factor in male mating success. here, we inv ...201627906074
larvicidal activity of green synthesized silver nanoparticles using excoecaria agallocha l. (euphorbiaceae) leaf extract against aedes aegypti.green synthesis of silver nanoparticles (agnps) using plant extracts has been achieved by eco-friendly reducing and capping agents. the present study was conducted to evaluate the larvicidal efficacies of agnps synthesized using aqueous leaf extracts of excoecaria agallocha against dengue vector, aedes aegypti. the 3(rd) and 4(th) instar larvae of a. aegypti were exposed to various concentrations of aqueous extracts of e. agallocha, synthesized agnps and also crude solvent extracts (methanol and ...201627906138
tracking insecticide resistance in mosquito vectors of arboviruses: the worldwide insecticide resistance network (win). 201627906961
human igg antibody response to aedes nterm-34kda salivary peptide, an epidemiological tool to assess vector control in chikungunya and dengue transmission area.arboviral diseases are an important public health concerns. vector control remains the sole strategy to fight against these diseases. because of the important limits of methods currently used to assess human exposure to aedes mosquito bites, much effort is being devoted to develop new indicators. recent studies have reported that human antibody (ab) responses to aedes aegypti nterm-34kda salivary peptide represent a promising biomarker tool to evaluate the human-aedes contact. the present study ...201627906987
mycobacterium ulcerans toxin, mycolactone may enhance host-seeking and oviposition behaviour by aedes aegypti (l.) (diptera: culicidae).the ecological functions of many toxins continue to remain unknown for those produced by environmental pathogens. mycobacterium ulcerans, the causative agent of the neglected tropical disease, buruli ulcer, produces a cytotoxic macrolide, mycolactone, whose function(s) in the environment remains elusive. through a series of dual-choice behaviour assays, they show that mycolactone may be an interkingdom cue for the yellow fever mosquito, aedes aegypti, seeking blood-meals as well as oviposition s ...201627907248
cocos nucifera coir-mediated green synthesis of pd nps and its investigation against larvae and agricultural pest.in recent decades, several scientists focused their process towards nanoparticles synthesis by using various sustainable approaches. cocos nucifera (c. nucifera) was one of the versatile trees in tropical regions which also can act as a thrust quencher in all over the world. cocos nucifera coir was one of the waste by-products in all coconut-refining industries and with the help c. nucifera coir, palladium nanoparticles (pd nps) were synthesized. green-synthesized spherical-shape pd nps were ove ...201627908201
response of three cyprinid fish species to the scavenger deterrent factor produced by the mutualistic bacteria associated with entomopathogenic nematodes.the symbiotic bacteria, photorhabdus and xenorhabdus associated with entomopathogenic nematodes (epns) in the genera heterorhabditis and steinernema, respectively, produce a compound(s) called the scavenging deterrent factor (sdf). sdf deters a number of terrestrial insect scavengers and predators and one bird species from feeding on host insects killed by the nematode-bacterium complex but has not been tested against aquatic vertebrates. moreover, the heterorhabditis-photorhabdus association is ...201727908637
rnai efficiency, systemic properties, and novel delivery methods for pest insect control: what we know so far.in recent years, the research on the potential of using rna interference (rnai) to suppress crop pests has made an outstanding growth. however, given the variability of rnai efficiency that is observed in many insects, the development of novel approaches toward insect pest management using rnai requires first to unravel factors behind the efficiency of dsrna-mediated gene silencing. in this review, we explore essential implications and possibilities to increase rnai efficiency by delivery of dsr ...201627909411
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