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sublethal effects of larval methoprene exposure on adult mosquito longevity.larvae of aedes aegypti were exposed to sublethal concentrations of the insect growth regulator, methoprene, and the glycogen content of pupae and surviving adults was compared and effects on adult longevity determined. the glycogen reserves in both male and female ae. aegypti pupae were significantly reduced as a result of methoprene exposure. the longevity of adult females was also significantly reduced, but exposure affected neither the longevity nor the glycogen content of adult males. adult ...19921402867
comparative efficacy of aphid extracts and some juvenoids against the development of mosquitoes.comparative efficacy of natural juvenile hormones extracted from aphis craccivora and a. gosypii and 5 juvenoids, i.e., methoprene, neporex, oms 3007, oms 3019 and dpe-28 on the development of aedes aegypti, anopheles stephensi and culex quinquefasciatus have been evaluated. oms 3007, oms 3019, dpe-28 and neporex show species specific related activities, while methoprene and aphid extracts do not show such activity against these mosquito species. treatment of mosquito eggs with an ec50 dose of t ...19921402861
relationship of hemolymph phenol oxidase and mosquito age in aedes aegypti.monophenol oxidase (mpo) and diphenol oxidase (dpo) activity in hemocytes and cell-free plasma perfused from 7-, 14-, 21-, and 28-day-old aedes aegypti mosquitoes were compared. a progressive decrease of enzyme activity was detected as mosquito age increased, and this decrease was significant in both hemocytes and cell-free plasma when mosquitoes were 28 days old as compared with that found in 7-day-old mosquitoes. there was no significant difference in total hemolymph protein as mosquito age in ...19921401989
[susceptibility of thirty-one species of mosquitoes to romanomermis yunanensis].a new species of mermithidae was found parasitizing the larvae of culex tritaeniorhynchus and culex fatigans in henan, china and then named romanomermis yunanensis. thirty-one species of mosquitoes involving six genera have been tested for susceptibility to r. yunanensis and culicinae mosquito have consistently been highly susceptible. at a 1:5 ratio of mosquito larvae to nematode juveniles, the parasitisms of aedes aegypti, ae. albopictus, culex fatigans, culex tritaeniorhynchus, anopheles sine ...19921363920
cannibalism and carnivory in toxorhynchites splendens (diptera: culicidae).laboratory reared larvae of toxorhynchites splendens, which were previously starved for 24 hours, cannibalized eggs of their own kind or preyed upon the eggs of other species (aedes aegypti and anopheles stephensi) present on the surface of water in small containers. second and third instars consumed eggs faster than first and fourth instars. the first instar consumed larvae of its own kind faster than the other instars, in the absence of other prey. however, when prey larvae were provided, ther ...19921362626
diel sugar-feeding and host-seeking rhythms in mosquitoes (diptera: culicidae) under laboratory conditions.mosquito sugar-feeding and host-seeking rhythms were recorded in the laboratory using remote sensors. in all five species studied, the two rhythms were nearly synchronous. in anopheles quadrimaculatus say, culex quinquefasciatus say, and aedes triseriatus (say), host-seeking activity increased slightly earlier than sugar feeding in the evening. the evening sugar-feeding period was longer than the evening host-seeking period in aedes aegypti (l.), but the two periods were of similar duration in a ...19921357175
genetic and molecular evidence for a trans-acting regulatory locus controlling glutathione s-transferase-2 expression in aedes aegypti.the amount of glutathione s-transferase-2 (gst-2) protein and enzyme activity in a mutant strain (strain gg) of the yellow fever mosquito (aedes aegypti) is approximately 25-fold higher than in the wild-type (++) strain. the mode of inheritance of the gg phenotype was studied in f1 and backcross progeny using gst enzyme assays, isozyme-specific antisera, and northern blot analysis. enzyme assay of parental and f1 progeny showed that the ++ phenotype was dominant to the gg phenotype. this was tru ...19921508145
biochemical and electrophoretical studies of the effect of some chemosterilants on theenzymes of aedes aegypti (l.) larvae.in vivo effect of standard and prospective chemosterilants on some enzymes is reported. colorimetric and histochemical findings indicate that alkaline phosphatase is inhibited by shikonin, hempa, tepa and shikonin angelate. cholinesterase is inhibited by all the compounds while diphenol oxidase is not inhibited. these findings have been discussed in the light of the earlier observations.199216414718
effect of the repellent deet on the antennal chemoreceptors for oviposition in aedes aegypti (diptera: culicidae).the insect repellent, n,n-diethyl-m-toluamide (deet), interferes with detection of the host attractant, lactic acid (la), by la-excited neurons on the antennae of female aedes aegypti (l.) mosquitoes. in the current laboratory behavioral experiment, the attraction of gravid female mosquitoes to a source of ethyl propionate, an oviposition attractant, was reduced by a factor of two when deet was present. similarly, when deet was presented together with ethyl propionate in electrophysiological exp ...19921495074
in vivo and in vitro biosynthesis and metabolism of methyl farnesoate, juvenile hormone iii, and juvenile hormone iii acid in the mosquito aedes aegypti.biosynthesis and metabolism of juvenile hormone (jh) iii in vivo and in vitro were studied in female aedes aegypti (l.). [12-3h]methyl farnesoate was used to follow the synthesis and [12-3h]-(10r)-jh iii to study metabolism. the rate of biosynthesis of [12-3h]jh iii in vivo after adult eclosion increased from 9 fmol/h per female at 1 h to 22 fmol/h per female at day 6. the rate of biosynthesis by exposed corpora allata (ca) in vitro was 23 fmol/h per ca during the 1st d after adult eclosion, the ...19921495071
mosquito repellent activity of oils from vitex negundo linn. leaves.oil obtained from stream distillate of v. negundo leaves was fractionated by column chromatography. mosquito repellence activity, as evaluated against aedes aegypti was mainly confined to the most polar fractions. the protection period against mosquito bites by polar fractions ranged between 1-3 h. however, the mean protection period values of these fractions did not show significant increase in the subsequent subfractions.19921398810
effects of toxorhynchites moctezuma larval predation on aedes aegypti populations: experimental evaluation.predatory larvae of the mosquito toxorhynchites moctezuma were used experimentally to control a standing crop of larvae of the dengue vector mosquito ae.aegypti. each week, fifty ae.aegypti first instar larvae were introduced to each of five water-filled drums (220 litres) of the type commonly used for domestic water storage in caribbean dwellings. at the beginning of the fourth week, a certain number (0, 1, 2, 5 or 10) of first instar tx.moctezuma larvae were introduced to each drum and the dai ...19921358268
[the action of phytobacteriomycin on the ultrastructure of the epithelial cells in the midgut of the mosquito aedes aegypti l]. 20091331734
interspecific variation in desiccation survival time of aedes (stegomyia) mosquito eggs is correlated with habitat and egg size.survival times of eggs under three humidity conditions (42%, 68%, 88% rh) were investigated among aedes (stegomyia) mosquitoes from temperate and tropical zones (5 species and 20 geographical strains). this subgenus tends to occupy small aquatic sites as larvae, where desiccation resistance of eggs is necessary during habitat drought. interspecific comparison showed that the egg survival time was correlated with egg volume and dryness of source locality, and probably with habitat. aedes aegypti ...199228313521
beta-cyfluthrin, a synthetic pyrethroid for mosquito control.beta-cyfluthrin (oms 3051), a new synthetic pyrethroid and one of the stereoisomers of cyfluthrin, was studied for insecticidal activity against eight mosquito species. its larvicidal activity with lc50 values of 5.62 x 10(-5) and 1.19 x 10(-4) mg/l respectively for culex quinquefasciatus and aedes aegypti was comparable with that of deltamethrin. this pyrethroid was more effective against the larvae of armigeres subalbatus (lc50 - 7.76 x 10(-7)) and the adults of anopheles culicifacies lt50 - 2 ...19921359650
reproductive and metabolic differences between aedes aegypti and ae. albopictus (diptera: culicidae).aedes albopictus (skuse) females were more likely to develop eggs after they ingested small blood meals than were aedes aegypti (l.) when both species were maintained under the same suboptimal adult nutritional regimen. the longevity of adult female ae. albopictus under conditions of starvation was also significantly greater than that of ae. aegypti. analyses of total body proteins, lipids, and glycogen indicated that the increased reproductive efficiency of ae. albopictus may be a result of its ...19921625295
aedes aegypti (diptera: culicidae) vision: response to stimuli from the optical environment.although aedes aegypti (l.) are capable of some wavelength discrimination, behavioral experiments demonstrated that color was not a stimulus to which they responded. optical stimuli that did affect ae. aegypti behavior were luminous reflectance, vertical contrast, and movement. stationary objects of low reflectance and solid color were the most attractive to males and females.19921625292
digestibility by and pathogenicity of the protozoa tetrahymena pyriformis to larvae of aedes aegypti. 19921607672
influence of short time exposure to an insect growth regulator, hexaflumuron, on mortality and adult emergence of vector mosquitoes.hexaflumuron, an insect growth regulator (igr), was found to greatly affect the development of immatures and emergence of adults of three species of vector mosquitoes, culex quinquefasciatus, aedes aegypti and anopheles stephensi, when larvae were subjected to short time exposure of < or = 1 h. this igr could completely prevent adult emergence even at a minimum exposure time of 10 min at 0.001, 0.01 and 0.1 mg/l. on treatment, larval and pupal mortality as well as varying degrees of morphogeneti ...20071308571
invasion of cemeteries in florida by aedes albopictus.aedes albopictus has been found in 53 of the 67 florida counties. the initial discoveries in 11 of these counties were made in cemeteries. at several locations, ae. albopictus became well-established in cemeteries before appearing in nearby accumulations of waste tires. the recycling of plastic floral baskets may be aiding the spread of ae. albopictus. mosquitoes were commonly found in all types of flower-holding containers in cemeteries, except bronze vases. in the laboratory, most aedes aegypt ...19921583479
in vitro assay for the biosynthesis and metabolism of juvenile hormone by exposed corpora allata of aedes aegypti (diptera: culicidae).a technique has been developed to study jh iii biosynthesis in vitro by removing the head and thorax of aedes aegypti (l.) and exposing the corpora allata (ca). exposed ca were incubated with [12-3h]methyl farnesoate, and the newly synthesized jh iii and jh iii metabolites were followed using c18 reversed-phase, high-performance liquid chromatography (hplc) and preparative gas chromatography. the rate of synthesis of [12-3h]jh iii by exposed ca from newly emerged adult ae. aegypti was 23 fmol/ca ...19921495050
aedes aegypti (diptera: culicidae) vision: spectral sensitivity and other perceptual parameters of the female eye.from measurements of the mean ommatidial diameter (17.2 microns) and mean interommatidial angle (6.2 degrees) of the eye of female ae. aegypti (l.), a minimum resolvable angle of 12.3 degrees and an eye parameter of 1.6 microns were calculated. the eye had relatively poor acuity but high overall sensitivity to light. electroretinographs revealed spectral sensitivity ranging from ultraviolet (323 nm) to orange-red (621 nm), with sensitivity peaks in the ultraviolet (lambda max323-345 nm) and gree ...19921495042
laboratory colonization and maintenance of toxorhynchites moctezuma.a colony of toxorhynchites moctezuma was established at the caribbean epidemiology centre in trinidad in 1984. toxorhynchites moctezuma was maintained in cages with high humidities. eggs were deposited most frequently in a cut bicycle tire containing water. a minimum of 42 h was required for hatching, but 94% hatched between 43 and 51 h. aedes aegypti larvae were supplied as prey. larval development times varied with the quantity of prey offered, but when fed ad lib, peak developmental time was ...19921349915
selection of the yellow fever mosquito aedes aegypti for cheap and easy maintenance without bloodmeals. 19921600234
infectivity and pathogenicity of ascogregarina culicis (eugregarinida: lecudinidae) to aedes aegypti (diptera: culicidae).the susceptibility of three strains of aedes aegypti (l.) to four strains of ascogregarina culicis were studied under laboratory conditions. the parasite was found to be pathogenic to the mosquito; although pathogenicity varied among geographical strains of a. culicis and susceptibility varied among geographical strains of ae. aegypti. in addition to affecting the survival of the mosquito larvae, infection with the parasite was found to shorten larval development time. selected strains of a. cul ...19921552514
the mosquitocidal activity of conidia of tolypocladium tundrense and tolypocladium terricola.tolypocladium tundrense and t. terricola uv-irradiated conidia exhibited acute toxicity to aedes aegypti larvae in concentrations of 5 x 10(5) and 5 x 10(6) ml-1, respectively. substances deposited on the surface of conidia following sporulation appear to be responsible for rapid intoxication and killing of mosquito larvae. treatment of larvae with dried methanolic extract of the spores also induced a high level of mortality accompanied by the same histological changes, including vacuolization a ...19921505208
generation of superoxide anion radical by alpha-terthienyl in the anal gills of mosquito larvae aedes aegypti: a new aspect in alpha-terthienyl phototoxicity.the present study documents that the secondary plant metabolites, especially alpha-terthienyl, exert phototoxic action through inhibition of certain enzymes and generation of singlet oxygen. some of the reports have emerged exhibiting involvement of free radical generation in vitro by alpha-terthienyl. we provide evidence for the generation of a free radical viz., superoxide anion radical, by alpha-terthienyl employing spin-trapping techniques, probably due to the extension of the latter reactio ...19921318115
permethrin and dimethyl phthalate as tent fabric treatments against aedes aegypti.tests were conducted to determine the persistence of compounds applied to tents to protect against mosquitoes. interiors of 2 camping tents were treated by the manufacturer--one with the repellent dimethyl phthalate (dmp) and one with the insecticide permethrin. a third tent was untreated. tents were set up outdoors and exposed to weathering for one year. weekly tests were conducted by releasing aedes aegypti into the tents, then recording knockdown (kd) and biting behavior during a 3-h period. ...19911787396
organization of a cloned repetitive dna fragment in mosquito genomes (diptera: culicidae).the structure and genomic organization of a cloned 5.2-kb repetitive dna fragment, h-85, isolated from the aedes albopictus genome have been examined. in situ hybridization of the 3h-labeled h-85 dna to the meiotic and mitotic chromosome preparations of ae. albopictus shows that the sequences homologous to h-85 dna are dispersed throughout the length of all three pairs of chromosomes. a similar pattern of in situ hybridization appears in aedes seatoi, aedes flavopictus, and aedes aegypti. the st ...19911685722
effect of ivermectin on the ovarian development of aedes aegypti (diptera: culicidae).the effect of ivermectin (0.1 microgram/ml) on blood digestion, ovarian development, and ovipositional attributes of aedes aegypti was studied using standard morphological and histological techniques. uncoordinated movements and paralysis were observed in most ivermectin-treated females within 1 h after ingestion of blood containing the chemical. eight days after the blood meal, 23.5% of the treated females had died, whereas no mortality occurred in controls. formation of the peritrophic membran ...19911941939
ribosomal dna structure in aedes mosquitoes (diptera: culicidae) and their cell lines.the molecular organization of the ribosomal dna repeat unit in laboratory colonies of aedes albopictus (skuse) and aedes aegypti (l.) and in continuous cell lines derived from these species was examined using restriction enzyme mapping and southern blotting techniques. in the rrna coding regions, restriction enzyme sites were conserved in ae. aegypti and ae. albopictus. in particular, sites that had been mapped in rdna cloned from the mauritius strain of ae. albopictus mosquitoes were confirmed ...19911941931
evaluation of australian mesocyclops (cyclopoida: cyclopidae) for mosquito control.six of seven species of mesocyclops copepods, collected from northeastern australia, showed potential as biological control agents of aedes mosquitoes. methods of laboratory evaluation included predation trials at different larval mosquito densities, population growth rates at different temperatures, and laboratory cage simulations. the predation efficacy of mesocyclops aspericornis, m. australiensis, and m. mb1 from southern queensland, and m. darwini, m. mb2, m. notius and m. mb3 from northern ...19911941928
replacement of aedes aegypti by aedes albopictus in mobile, alabama.aedes albopictus was first detected in mobile, al, in 1987 during a cdc sponsored ovitrap survey in the historic district. a comparison of ovitrap and larval surveys, done in 1957, 1984, 1987 and 1990, indicates that ae. albopictus had replaced aedes aegypti in urban mobile. possible explanations of this replacement, including displacement, are discussed.19911791461
emergency control of aedes aegypti as a disease vector in urban areas.techniques for the emergency control of adult aedes aegypti populations and their development are reviewed. larviciding and environmental measures provide only delayed control of adult populations. large-scale field trials of the ultra-low volume application of insecticide concentrates in southeast asia, south america and africa, using aerial, ground, vehicle-mounted and hand-carried equipment, have, in most cases, resulted in satisfactory levels of control of adult populations. sequential or in ...19911791444
tralomethrin (oms-3048), a new synthetic pyrethroid against vector mosquitoes.tralomethrin (oms-3048) a synthetic pyrethroid, was tested for its insecticidal properties in the laboratory against normal strains of culex quinquefasciatus, cx. tritaeniorhynchus, cx. sitiens, anopheles stephensi, an. culicifacies, aedes aegypti and armigeres subalbatus and against strains of cx. quinquefasciatus resistant to fenthion and malathion. tralomethrin showed good larvicidal activity against all the species tested. lc50 ranged between 7.00 x 10(-6) and 9.10 x 10(-3) mg (ai)/l. resist ...19911688021
pathogenicity, host range and temperature tolerance of crypticola clavulifera (oomycetes: lagenidiales) in the laboratory.the oomycete fungus crypticola clavulifera was pathogenic for laboratory-reared and field-collected larval diptera, including the mosquitoes aedes aegypti, ae. notoscriptus, anopheles farauti no. 1 and 3, culex annulirostris, cx. quinquefasciatus and the chironomid midge chironomus tepperi. zoospores were produced in water held at 15, 20 and 25 degrees c and were pathogenic for ae. aegypti larvae, but at 30 and 35 degrees c zoospores were not produced.19911686451
dibutyryl camp activates bumetanide-sensitive electrolyte transport in malpighian tubules.the effects of dibutyryl adenosine 3',5'-cyclic monophosphate (dbcamp) and bumetanide (both 10(-4) m) on transepithelial na+, k+, cl-, and fluid secretion and on tubule electrophysiology were studied in isolated malpighian tubules of the yellow fever mosquito aedes aegypti. peritubular dbcamp significantly increased na+, cl-, and fluid secretion but decreased k+ secretion. in dbcamp-stimulated tubules, bumetanide caused na+, cl-, and fluid secretion to return to pre-camp control rates and k+ sec ...19911653525
the gonotrophic status of female aedes aegypti (l.) overnight at the oviposition site (diptera: culicidae).experimental observations made outdoors in trinidad provide additional support for the assumption that some gravid female aedes aegypti rest overnight close to the container in which each has laid part of her current complement of eggs the previous afternoon, and in which she may lay part or the rest of that complement the next morning. some females evidently take more than one blood-meal while developing a single batch of eggs.19911796890
influence of larval and adult nutrition on biting persistence in aedes aegypti (diptera: culicidae).biting persistence was measured for aedes aegypti (l.) mosquitoes given different levels of larval and adult nutrition. persistence was measured as the total number of times during a 60-min trial that a mosquito returned to land on a defensive host after being disturbed and dislocated from the host, and as the rate of decline of landings over the 60 min test (pattern of persistence). large females were significantly more persistent than smaller females, regardless of whether the females were pro ...19911941913
larvicidal activity of tagetes minuta (marigold) toward aedes aegypti.the steam distilled oils of 3 species of marigold, tagetes patula, t. erecta and t. minuta, were tested for larvicidal activity toward third instar aedes aegypti; activity at 10 ppm was demonstrated only for t. minuta. the larvicidal property of the whole oil dispersed in water persisted for at least 9 days. the terpene, ocimenone, which is a part of the whole oil, was found to be larvicidal only at a higher concentration than the whole oil and to lose its activity within 24 h after dispersal in ...19911895085
residual concentration and efficacy of three temephos formulations for control of larval aedes aegypti.the residual concentration and efficacy of abate 5cg (impregnated in celatom granules) and plaster pellets impregnated with either abate 4e or technical temephos were compared against late 3rd-instar aedes aegypti larvae. both plaster pellet formulations resulted in 100% larval mortality during the 6-wk test, compared with 2 wk for a similar level of mortality for the celatom formulation. the maximum temephos concentration in water treated with the celatom formulation occurred 30 min after treat ...19911716660
involvement of mono-oxygenases as a major mechanism of deltamethrin-resistance in larvae of three species of mosquitoes.role of mono-oxygenases as a mechanism of resistance to the synthetic pyrethroid, deltamethrin in the larvae of culex quinquefasciatus say, aedes aegypti l. and anopheles stephensi liston developed by laboratory selections with deltamethrin, ddt or deltamethrin and the synergist, piperonyl butoxide (pbo) in the ratio of 1:5, was investigated. there was a significant correlation with mono-oxygenase activity and larval lc50 to deltamethrin in various strains of all the three species. in addition, ...19911678732
enhancement of the cdc ovitrap with hay infusions for daily monitoring of aedes aegypti populations.an ovitrap containing hay infusion and a second ovitrap adjacent to it containing a 10% dilution of the infusion in tap water together yielded 8 times more aedes aegypti eggs than single cdc ovitraps containing tap water. these "enhanced pairs" were significantly more attractive than pairs with other combinations of infusion, water or methyl propionate, and have proven useful for daily monitoring of ae. aegypti populations. our results shed light on the oviposition behavior of ae. aegypti in the ...19912045808
effects of single introduction of toxorhynchites moctezuma upon aedes aegypti on a caribbean island.in march 1989, first instar toxorhynchites moctezuma larvae were introduced into all potential aedes aegypti oviposition sites (n = 214) that contained water in the village of clifton on union island in st. vincent and the grenadines. one month after this introduction, the mean number of adult ae. aegypti collected with a backpack aspirator in houses in clifton dropped significantly. sixty-four percent fewer males and 80% fewer females were collected after introduction of the predator. in the un ...19911675259
compounds affecting mosquito oviposition: structure-activity relationships and concentration effects.evidence is reviewed for a dose-dependent reversal of response in mosquito oviposition, in which a compound that attracts or stimulates oviposition may repel or deter oviposition at a higher concentration. on the basis of a review of structure-activity relationships in compounds affecting mosquito oviposition, 5 hexanoic acid derivatives were selected for field tests with aedes aegypti ssp. formosus in kenya. egg counts were increased most by methyl hexanoic acid and 5-methyl-2-hexanone, and the ...19911675257
isolation and characterization of the gene expressing the major salivary gland protein of the female mosquito, aedes aegypti.we have undertaken a molecular analysis of the salivary glands of hematophagous insects in order to better understand their role in blood feeding and in the transmission of infectious diseases. to that end, genomic and cdna clones of a gene designated d7, expressed abundantly in the adult female salivary glands of the vector mosquito aegypti, have been isolated and characterized. this gene encodes a mrna shown by northern analysis and in situ hybridization to tissue sections to be specifically t ...19912052024
plagiorchis noblei (plagiorchiidae) in aedes aegypti: parasite acquisition and host mortality in trickle infections.the prevalence and intensity of experimental infections of aedes aegypti with the digenean plagiorchis noblei increased significantly with the level of trickle exposure to cercariae. daily exposure to doses of 16 cercariae/day yielded a mean infection intensity of 13.0 metacercariae; doses of 1 cercaria/day resulted in only 2.4 metacercariae per infected mosquito larva. the prevalence of infection rose from 46% at an exposure of 1 cercaria/day to 99% at 16 cercariae/day. host mortality rose conc ...19911992081
toxicity and igr effect of two new neem products against aedes aegypti (pcsir strain).crude extract and isolated compounds from neem, azadirachta indica and melia azedarach (meliaceae) are being tested in various countries. the present study deals with the effect of two new compounds and their parent fraction from the fresh winter neem leaves, isolated by siddiqui et. al., 1986. the lc50 of nfd is 0.58 ppm, nimocinolide 0.625 ppm and isonimocinolide 0.47 ppm. abnormal larvae, pupae and intermediates were also found.199116414684
control of follicular epithelium development and vitelline envelope formation in the mosquito; role of juvenile hormone and 20-hydroxyecdysone.using microsurgical manipulations, hormone applications, and transmission electron microscopy we have investigated the regulation of differentiation of the follicular epithelium and formation of the vitelline envelope (ve) in primary follicles in the ovary of the mosquito, aedes aegypti. during the first 3 days after eclosion, the primary follicle grows, and cells of the follicular epithelium differentiate, their content of mitochondria, rough endoplasmic reticulum, and golgi complexes increases ...19911926140
superoxide dismutase in the anal gills of the mosquito larvae of aedes aegypti: its inhibition by alpha-terthienyl.the anal gills of the mosquito larvae of aedes aegypti were shown to possess superoxide dismutase (ec 1.15.1.1) activity, which increased with the maturation of the larvae from instar 1 to instar 4. this enzyme was highly inhibited upon treatment of the larvae with alpha-terthienyl (2,2':5,2"-terthiophene) and subsequent exposure to long-wave ultraviolet light. inhibition also occurred with treatment of the crude enzyme extract in a similar fashion. exposure of the enzyme to the ultraviolet ligh ...19911799676
vector immunity to filariasis: new potential for disease control?these experiments describe investigations into the mode of microfilarial killing in the haemocoel of immune aedes aegypti and simulium ornatum. antibody blocking experiments indicate that sarcotoxin iia-like and sarcolectin-like molecules maybe involved. the use of carbohydrate in the infecting blood meal indicates that gut lectins may be involved in preventing migration of brugia microfilariae into the haemocoel. this appears to be a strain factor linked to innate susceptibility. these physiolo ...19911793268
n,n-diethylphenylacetamide in treated fabrics as a repellent against aedes aegypti and culex quinquefasciatus (diptera: culicidae).the efficacy of fabrics impregnated with n,n-diethylphenylacetamide (depa), a new repellent, was studied in the laboratory and field against aedes aegypti (l.) and culex quinquefasciatus say. depa-treated polyester cotton and cotton fabrics provided repellency for 30 and 36 d and had a half-life of 11 and 5 d, respectively. the repellent could not be applied at a rate of greater than 15% of fabric weight. depa-treated fabric patches were found to be a practical and novel approach and were effect ...19911674544
efficacy of encapsulated lagenidium giganteum (oomycetes: lagenidiales) against culex quinquefasciatus and aedes aegypti larvae in artificial containers.presporangial mycelia of lagenidium giganteum cultured on sunflower seed extract were encapsulated in calcium alginate and added once (july 18) to outdoor (raleigh, nc) caged tires, wood and concrete containers populated with first instars of culex quinquefasciatus or aedes aegypti. first instars were added twice weekly (for 10 wk) to simulate natural oviposition. the fungus persisted for 10 wk and recycled in the mosquito larvae of both species. the overall reductions of cx. quinquefasciatus an ...19902098480
genetic analysis of rock hole and domestic aedes aegypti on the caribbean island of anguilla.genetic variation was characterized at 11 enzyme coding loci in aedes aegypti collected from 3 rock hole and 4 domestic sites on the island of anguilla, west indies. the pattern of gene frequency variation suggests that these mosquito samples do not constitute a single panmictic population, but there are no large consistent differences between rock hole and domestic forms to parallel the east african sylvan-domestic dichotomy. with the exception of one of the domestic populations, two loci did h ...19902098468
culiseta minnesotae and further notes on aedes aegypti in rhode island.culiseta minnesotae is reported as a state record in rhode island. follow-up surveys for aedes aegypti after its initial isolation in 1985 have failed to recover additional specimens.19901983024
effects of sublethal dosages of abate upon adult fecundity and longevity of aedes aegypti.sublethal concentrations of abate (temephos) were applied to f2 generation aedes aegypti larvae, and fecundity and longevity were recorded in the emerged adults. females exposed to abate oviposited only in the first 2 gonotrophic cycles, meanwhile control females laid a few eggs after taking the third blood meal. dosages of 0.009, 0.013 and 0.015 mg/liter of abate decreased the mean egg production per gonotrophic cycle 37, 47 and 69%, respectively, in relation to the control. females that were e ...19901711574
electrochemical determination of diphenol oxidase activity using high-pressure liquid chromatography.a quantitative assay for the diphenol oxidase activity of tyrosinase (ec 1.14.18.1) using high-pressure liquid chromatography with electrochemical detection is described. the assay is based on the observation (m. sugumaran, 1986, biochemistry 25, 4489-4492) that tyrosinase catalyzes the oxidative decarboxylation of 3,4-dihydroxymandelic acid to 3,4-dihydroxybenzaldehyde. the substrate and product were readily separated on a reverse-phase column equilibrated with 0.1 m citrate buffer, ph 3.2, con ...19902127163
a night-time role of the oviposition site of the mosquito, aedes aegypti (l.) (diptera: culicidae).experimental observations taken outdoors in trinidad are consistent with the assumption that the oviposition site is used by some gravid female aedes aegypti as a nocturnal resting site prior to their laying eggs in it the next morning: the morning wave of oviposition of the typically bimodal diel periodicity was missing whenever an oviposition site was closed throughout the previous night. the diel pattern of oviposition did not differ significantly in sites at, and four feet above, ground leve ...19902256766
intradermal immunotherapy in children with severe skin inflammatory reactions to aedes aegypti and culex quinquefasciatus mosquito bites. 19902242953
studies onbacillus thuringiensis h-14 strains isolated in egypt-i. screening for active strains.screening for spore-forming bacteria to use against dipterous insects, namelymusca domestica, aedes aegypti andculex pipiens from egypt, led to two isolates, m1 and s128, being found which were highly toxic to bothaedes andculex strains. they had calculated international toxicity units (itu) valdes of 17,467 and 20,065 itu/mg, respectively, as compared with 15,000 itu/mg forbacillus thuringlensis h-14 ips-82 reference standard. they were taxonomically classilled asbacllus thuringlensis h-14. the ...199024430070
mosquito oostatic factor: a novel decapeptide modulating trypsin-like enzyme biosynthesis in the midgut.a peptide that inhibits egg development in mosquitoes (oostatic factor) has been purified from the ovaries of female aedes aegypti. the factor is a decapeptide with a molecular mass of 1047.6. the primary sequence has been determined as nh2-tyr-asp-pro-ala-pro-pro-pro-pro-pro-pro-cooh from mass spectra recorded on a quadrupole fourier transform instrument. the amino acid sequence exhibits sequence correlation to mammalian, plant, and several viral proteins. injection of synthetic analogs into mo ...19902394318
fecundity, metabolism, and body size in anopheles (diptera: culicidae), vectors of malaria.in four anopheles species, an. albimanus, an. gambiae, an. stephensi, and an. quadrimaculatus say, total protein, lipid, and carbohydrate present at eclosion, after feeding on sucrose, and after extreme starvation were quantified to study the effect of teneral and maximal reserves on subsequent fecundity and to judge the extent of reserve mobilization and the minimal irreducible amounts required for survival. all parameters were regressed on body size, presented as the cubic value of wing length ...19902231621
routine blood-feeding of aedes aegypti via a new membrane. 19902230787
attraction of mosquitoes to diethyl methylbenzamide and ethyl hexanediol.studies by prior workers have shown that insect repellents can act as attractants when present as low concentrations, deposits or residues. in the present study deet and ethyl hexanediol were tested in 2-fold serial doses from 1.9 x 10(-9) to 1.6 x 10(-2) mg/cm2 on the forearms of volunteers against colonized anopheles albimanus, aedes aegypti and ae. taeniorhynchus. both compounds were significantly repellent at the high end of the dose range, as expected. neither was significantly attractant t ...19902230775
differences in the larval alarm reaction in populations of aedes aegypti and aedes albopictus.the time spent submerged during the larval alarm reaction was measured for several strains of aedes aegypti and ae. albopictus from different locations. differences between the 2 species were not significant. differences in strains within the species were highly significant. greater differences were seen among strains of ae. albopictus than among strains of ae. aegypti. these differences represent an additional indication of extensive local differentiation in ae. albopictus.19902230770
correlation of nutritional reserves with a critical weight for pupation in larval aedes aegypti mosquitoes.the relationships between the readiness of fourth instar aedes aegypti to metamorphose and their weight, nutritional reserves and sex were examined. both the critical larval weight required for metamorphosis and the potential weight were influenced by the temperature at which the larvae were reared, and were greater for females than for males. caloric levels of soluble carbohydrates and soluble lipids correlated strongly with weight. the accumulations of both carbohydrates and lipids were sex-de ...19902230767
resistance of permethrin to weathering in fabrics treated for protection against mosquitoes (diptera: culicidae).two different methods of treating cotton and nylon-cotton fabrics with permethrin were evaluated for protection from mosquito bites after laboratory weathering. cotton fabric treated by the individual dynamic absorption method provided consistently better protection than cotton fabric treated by the aerosol method. the nylon-cotton fabric provided similar protection regardless of the treatment method. after weathering, the toxic effects of both types of permethrin-treated fabrics treated by both ...19901974929
evaluation of the control of mosquitoes with insect growth regulators.in this study, the effectiveness of eight insect growth regulators (igrs) (chlorfluazuron, diflubenzuron, el-494, flufenoxuron, teflubenzuron, juglone, plumbagin and methoprene) against five mosquito vectors (armigeres subalbatus, aedes albopictus, aedes aegypti, culex tritaeniorhynchus, and culex quinquefasciatus) was investigated in the laboratory. the ec50s of chlorfluazuron, diflubenzuron, el-494, flufenoxuron, teflubenzuron, and methoprene against the five mosquitoes ranged from 0.0001 to 0 ...19902402025
substitute blood meal for investigating and maintaining aedes aegypti (diptera: culicidae).a defined substitute blood meal has been developed for feeding aedes aegypti (l.), a mosquito that normally requires a vertebrate blood meal to produce eggs. this substitute blood meal is a simple mixture of proteins, with salts and adenosine triphosphate added to induce gorging. protein appears to be the only nutritional requirement. the mixture consists of gamma-globulins to initiate the hormonal responses necessary for normal egg development, hemoglobin as a visual marker of feeding, and albu ...19902388248
comparative functional morphology of the mouth brushes of mosquito larvae (diptera: culicidae).structural and morphological features of the feeding brushes of fourth instars of four species of mosquitoes were studied using light and scanning electron microscopes. the fine structure of the elements of the lateral palatal brushes, anteromedian palatal brushes, mandibular brushes and combs, and maxillary brushes of each species was investigated and related to feeding behavior. the movement of the mouth brushes in living larvae was observed by stereomicroscopy under laboratory conditions. mou ...19902388219
the effects of single exposures of aedes aegypti larvae and pupae to plagiorchis noblei cercariae in the laboratory.the mortality of aedes aegypti pre-imagos harboring metacercariae of plagiorchis noblei park, 1936, is governed by the stage of development of the host at the time of infection and the location of the parasite in the insect body. first and second instar larvae generally succumbed to infection, regardless of site. infections of the head and thorax of third and fourth instar larvae were generally lethal or gave rise to imparied adults. however, older instars frequently survived abdominal infection ...19902352060
insecticidal activity of carbosulfan (oms 3022) and pyraclofos (oms 3040) against mosquitoes.carbosulfan (oms 3022), a carbamate and pyraclofos (oms 3040), an organophosphate were evaluated for their biological activity against vector mosquitoes, both in laboratory and field conditions. carbosulfan (25% ec), was found to be active against adults and immatures of culex quinquefasciatus, anopheles stephensi and aedes aegypti. the ld values for adults were 6.65, 5.19 and 8.35 micrograms/cm2 and lc 50values for immatures were 5.4 x 10(-5), 1.6 x 10(-4 and 5.4) x 10(-4) 50mg/1 respectively. ...19901983012
studies on prophenoloxidase activation in the mosquito aedes aegypti l.this study, the first of its kind in a mosquito vector species, demonstrates the feasibility of studying prophenoloxidase activation in an insect containing not more than a few microliters of hemolymph. mosquito phenoloxidase was found to be in an inactive proenzyme form, prophenoloxidase. mosquito prophenoloxidase required bivalent cation for its activation; ca2+ was found to be the most efficient for activation. concomitant amidase activity was also observed prior to phenoloxidase activity. th ...19902110057
relative potency of lambdacyhalothrin and cypermethrin applied as thermal fogs for the control of houseflies (musca domestica) and mosquitos (aedes aegypti).two highly active synthetic pyrethroid insecticides, lambdacyhalothrin and cypermethrin, were evaluated as thermal fogs against houseflies (musca domestica linnaeus) and mosquitos (aedes aegypti linnaeus). lambdacyhalothrin (oms 3021) showed an average of 2.5 times more knockdown activity and over 5 times more adulticidal activity than cypermethrin against musca domestica and aedes aegypti. these results demonstrate that lambdacyhalothrin is highly effective at very low rates as a thermal fog ag ...19902402678
age dependence of salivary bacteriolytic activity in adult mosquitoes.1. bacteriolytic activity in the salivary glands of adult aedes aegypti female mosquitoes starts from a low level upon emergence, increases 6-fold over the first 3 days, then levels off. 2. mosquitoes start sugar-feeding after 2 days, when lytic activity is one-half of the peak level. 3. bacteriolytic activity in salivary gland extracts is not influenced by removal of corpora allata.19902390862
determination by near-infrared reflectance spectroscopy of mosquito (diptera: culicidae) bloodmeal size.a method is described for using near-infrared reflectance spectroscopy (nirs) to quantify the amount of blood present in mosquitoes without killing insect. nirs is a nonconsumptive, instrumental method for fast, accurate, and precise evaluation of chemical composition of material with little or no preparation. the nirs method accurately predicted bloodmeal size and bloodmeal disappearance in aedes aegypti (l.) and ae. sierrensis (ludlow) mosquitoes.19902299659
[evaluation of larvicide susceptibility of culicidae in french polynesia].larval tests on 4th-stage aedes aegypti, aedes polynesiensis and culex quinquefasciatus of french polynesia, according to the who method, demonstrate susceptibility of these species to organophosphorous compounds and less susceptibility to insect growth regulators.19901976447
the ribosomal genes of the mosquito, aedes aegypti.the characterisation of the ribosomal genes of the mosquito, aedes aegypti, is described. preliminary experiments using a cloned drosophila ribosomal dna (rdna) repeat to probe southern transfers of ae. aegypti genomic dna has indicated that the rdna repeat of ae. aegypti is 9.0 kb in length and that individual rdna repeats exhibit a high degree of homogeneity with respect to length and the position of restriction enzyme recognition sites within the rdna. the preliminary mapping data together wi ...19892573528
interspecific competition among aedes aegypti, ae. albopictus, and ae. triseriatus (diptera: culicidae): larval development in mixed cultures.interactions among the larvae of aedes aegypti (l.), ae. albopictus (skuse), and ae. triseriatus (say) were studied in trispecific and bispecific mixed populations under laboratory and field conditions. competitive stress (as evidenced by the average time to first, 50, and 75% pupation and the total pupation periods for mixed populations of each species in comparison with their single species controls) was more pronounced in mixed cultures reared in glass jars in the laboratory than in tires und ...19892585456
the effects of host distributional patterns on parasite transmission: aedes aegypti larvae and plagiorchis noblei cercariae.the effects of distributional patterns of the host on the acquisition of plagiorchis noblei cercariae by aedes aegypti larvae were determined. mosquito larvae that were allowed to disperse were more susceptible to infection than confined larvae. because these mosquito larvae are known to aggregate in light and disperse in darkness, they are more likely to acquire p. noblei infections at night. the timing of cercarial emergence in relation to the distributional patterns of the mosquito host is di ...19892795386
variation in four acid hydrolase activities in filarial-susceptible and -refractory genotypes of aedes aegypti. 19892695060
a new black tarsomere allele in aedes aegypti (diptera: culicidae) from the island of grand comoros. 20032794474
interspecific mating between louisiana strains of aedes albopictus and aedes aegypti in the field and laboratory.interspecific mating between aedes albopictus males and ae. aegypti females was detected in the field using mark-release-recapture techniques. by 3 days after the release of virgin ae. aegypti females into a field site containing only ae. albopictus, 100% of the captured females were inseminated. laboratory investigations indicated that male ae. albopictus were very proficient at inseminating ae. aegypti females and that ae. aegypti males rarely inseminated ae. albopictus females, especially if ...19892584975
structure-activity relationships in diphenyl ethers for insect growth regulating activity against mosquitoes.out of the 30 substituted diphenyl ethers synthesized and tested for insect growth regulating (igr) activity against mosquitoes, three compounds viz., dpe-16, 19 and 28 showed promising igr activity. while dpe-16 and 19 were found to be effective against all three vector species tested viz., culex quinquefasciatus, aedes aegypti and anopheles stephensi, with the respective ei50 values of 0.1485, 0.3650, 0.2225 mg/l and 0.1474, 0.1392, 0.1145 mg/l, dpe-28 was found to be highly effective against ...19892576420
a laboratory investigation of mosquito larval predation by toxorhynchites moctezuma on aedes aegypti.1. functional responses of predatory toxorhynchites moctezuma (dyar & knab) larvae feeding on aedes aegypti (l.) larvae (diptera: culicidae) were found to be type ii of holling (1959) and rogers (1972). 2. estimates of searching rate were generally higher for later instar predators. the search rate of second instar predators declined as prey instar increased, but fourth instar tx. moctezuma had the highest search rate for second instar ae. aegypti. 3. prey handling times were higher for early in ...19892577517
plagiorchis noblei in aedes aegypti: cercarial age and infectivity. 19892746212
effects of weathering on fabrics treated with permethrin for protection against mosquitoes.permethrin-impregnated and untreated fabrics were evaluated for their toxic and repellent effects against anopheles stephensi and aedes aegypti after both types of fabrics were subjected to accelerated weathering for 9 weeks, under a simulated wet/tropical environment. the toxic (knockdown) effect of permethrin-impregnated fabrics against both species of mosquitoes diminished rapidly after 1 week compared to the repellent effect. after 6 weeks of weathering, the remaining low amounts of permethr ...19892568392
culicidae (diptera) mortality resulting from insecticide aerosols compared with mortality from droplets on sentinel cages.the mortality of female aedes aegypti exposed to an ultralow-volume (ulv) aerosol for 15 min and then transferred to clean cages was compared to the mortality of females exposed to the contaminated sentinel exposure cages. ulv aerosol sprays of fenitrothion (50% ai) were dispersed at 180 and 205 ml/min, and ulv aerosol sprays of bendiocarb (18.87% ai) were dispersed at 90 and 120 ml/min in an open field. at 24 h after exposure, the mean percentage of mortality of ae. aegypti in cages previously ...19892566687
more on recombination and selection in the modifier theory of sex-ratio distortion.g. maffi and s.d. jayakar suggested a model for the two-locus control of sex determination in the mosquito aedes aegypti (1981, theor. pop. biol. 19, 19-36). this model was extended to multiple alleles and analyzed in mathematical detail by s. lessard (1987, theor. pop. biol. 31, 339-358). the model supposes that males are "mm" and females "mm" but the transmission from males is controlled by a second gene with alleles ai. we show that in addition to the equilibrium in which mai in females, mai ...19892727954
reduced productivity in adult yellowfever mosquito (diptera: culicidae) populations.male and female aedes aegypti (l.) mosquitoes of the laboratory strain rock were irradiated with 130 mw of argon 514.5 nm laser microbeams for 0.04, 0.25, 0.4, and 0.5 s, respectively. egg production, percentage hatch, and productivity (average number of adults surviving after 3 wk) were used to assess mutagenic effects. mortality was high for males in all laser radiation groups and increased with time of exposure. except for the group treated for 0.25 s, significant reductions in total f1 proge ...19892708628
[biological activity of precozen i on musca domestica and aedes aegypti].paper presents the results of the study of biological effect of precozen i--preparation with antijuvenile type of action on flies and mosquitoes. parenteral administration resulted in reduced rate of imago hatching, alterations in female reproductive system, hereditary sterility. treatment of flies' as well as mosquitoes' larvae caused various moult alterations: prologation of development stages, reduced chitinization. multiple effects of precozen i on insects, characteristic both of hormonal pr ...20092755407
impact of new insect growth regulators and their formulations on mosquito larval development in impoundment and floodwater habitats.four insect growth regulators were evaluated in the laboratory and field. in the laboratory, ac-291898 showed excellent activity, inducing 90% mortality in culex quinquefasciatus and aedes aegypti at 0.5-0.7 ppb. the ec formulation of xrd-473 was slightly more active than the technical material with an lc90 of 0.84 ppb and 0.92 ppb against ae. aegypti and cx. quinquefasciatus, respectively. in the field, ac-291898 at the rates of 0.005 and 0.01 lb ai/acre caused 85 and 100% inhibition of adult e ...19892565363
the relationship between microfilarial density and uptake of foleyella candezi microfilariae by aedes aegypti.there was a linear regression in the square root of the numbers of microfilariae abstracted (square root of y) by aedes aegypti refm strain on the square root of the density of microfilariae in the infecting blood (square root of x). the linear regression is represented by the equation, square root of y = 0.080,59 square root of x + 4.556 (r = 0.627; p less than 0.01). - ae. aegypti abstracted microfilariae about 1.60 times that expected from the infecting blood meal. this value however decrease ...19892729645
selective expression of phosphodiesterase ii during ovarian development in aedes aegypti. 19892541933
cultural factors in aedes aegypti and dengue control in latin america: a case study from the dominican republic.dengue and aedes aegypti infestation in latin america pose substantial health risks. total reliance on chemical interventions is no longer feasible and, increasingly, programs of control will need to rely on education, behavior change and community participation. findings from applied behavioral research in the dominican republic conducted from july through september of 1987 are discussed in the light of cultural constraints on intervention efforts. perception of risk, the choices for curative c ...198920840930
genetic transformation of the mosquito aedes aegypti by micro-injection of dna.we report the successful introduction of heterologous dna sequences into embryos of the mosquito aedes aegypti (l.) by microinjection. the injected dna carried p transposable element sequences, derived from and known to facilitate transformation in drosophila melanogaster. two plasmids, one of which carried a dominant selectable marker, were introduced into the posterior of embryos prior to pole cell formation and subsequently taken up into the germ line of transformed individuals. stable transf ...19892519641
phenotypic variability and heterozygosity at an esterase locus in the mosquito aedes aegypti.populations of the yellow fever mosquito, aedes aegypti l. are polymorphic for esterase alleles. this could be because the alleles are neutral or because selection protects the polymorphism, as had been concluded from a study of allele frequencies. a test has been carried out to look for possible associations of esterase heterozygosity with speed of development, body size and spine number symmetry in two spine rows. heterozygotes were found to be larger and slower growing than homozygotes, but t ...19883230031
phototaxis in aquatic invertebrates: possible use in ecotoxicity tests.a series of experiments has been carried out aimed at (i) a deeper study of the phototaxis of some aquatic invertebrates and (ii) its possible use in ecotoxicity tests. for the first point the phototaxis of the nauplii of artemia salina was examined: through tests carried out under different conditions, it was observed that a marked complex response to light existed, which led to a subdivision between individuals with different phototactic response, mainly depending on the density of the nauplii ...19883229377
the biology of aedes albopictus.the biology of aedes albopictus is reviewed, with emphasis on studies of ecology and behavior. the following topics are discussed: distribution and taxonomy, genetics, medical importance, habitat, egg biology, larval biology, adult biology, competitive interactions, comparative studies with aedes aegypti, population dynamics, photoperiodism, and surveillance and control.19883068349
toxicity, localization and elimination of the phototoxin, alpha-terthienyl, in mosquito larvae.mosquito larvae were examined to determine interspecific and interstrain differences in susceptibility to the larvicidal effects of the plant-derived phototoxin, alpha-terthienyl (alpha-t). the lc50 values were as follows: aedes aegypti, 4 ppb; ae. epactius, 6 ppb; anopheles stephensi, 14 ppb; malathion-susceptible culex tarsalis (s), 12 ppb; malathion-resistant cx. tarsalis (r), 16 ppb. fluorescence studies indicated localization of alpha-t in the midgut epithelium and in the lumen of malpighia ...19882906359
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