| [utilization of the waste of sisal industry in the control of mosquito larvae]. | the aim of this research was to utilize the waste residues of sisal fiber separation from agave sisalana leaves to develop a larvicide for the combat of mosquito transmitting tropical diseases. larvae of aedes aegypti and culex quinquefasciatus were exposed to different concentrations of the agave extract for 24 hours to determine lethal concentrations. the lc50 for a. aegypti was 322 ppm and the lc50 for c. quinquefasciatus was 183 ppm. to detect the active substances, saponins were investigate ... | 2006 | 9927821 |
| precise excision and transposition of piggybac in pink bollworm embryos. | transposable elements such as p, hobo, hermes, mariner and minos have been successfully harnessed as gene vectors to achieve the transformation of several dipteran species including drosophila melanogaster, ceratitis capitata and aedes aegypti. plasmid-based excision and transposition assays have been useful indicators of an element's ability to be mobilized in vivo and thus potentially serve as a transforming vector. we report that the transposable element piggybac is capable of precise excisio ... | 1999 | 9927180 |
| insect immunity: molecular cloning, expression, and characterization of cdnas and genomic dna encoding three isoforms of insect defensin in aedes aegypti. | aedes aegypti were immune activated by injection with bacteria, and the expression of insect defensins was measured over time. northern analyses indicated that defensin transcriptional activity continued for at least 21 days after bacterial injection, and up to 10 days after saline inoculation. mature defensin levels in the haemolymph reached approximately 45 microm at 24 h post inoculation. cdnas encoding the preprodefensins of three previously described mature ae. aegypti defensins were amplif ... | 1999 | 9927179 |
| molecular cloning and expression of two hexamerin cdnas from the mosquito, aedes aegypti. | fourth-instar larvae of aedes aegypti synthesize two types of hexamerins, hexamerin-1 (aahex-1) and hexamerin-2 (aahex-2), whose subunits are distinguished by different methionine and aromatic amino acid contents. in early female pupae only the methionine-rich aahex-1gamma subunit accumulates to two-fold higher levels than in males. to investigate the relationship between hexamerin structure and the roles of hex-1 and hex-2 during mosquito development and reproduction, we have cloned and sequenc ... | 1999 | 9927174 |
| spatial distribution of insecticide resistance in caribbean populations of aedes aegypti and its significance. | to monitor resistance to insecticides, bioassays were performed on 102 strains of the dengue vector aedes aegypti (l.) from 16 countries ranging from suriname in south america and through the chain of caribbean islands to the bahamas, where the larvicide temephos and the adulticide malathion have been in use for 15 to 30 years. there was wide variation in the sensitivity to the larvicide in mosquito populations within and among countries. mosquito strains in some countries such as antigua, st. l ... | 1998 | 9924507 |
| biochemical analysis of a blood meal-induced aedes aegypti glutamine synthetase gene. | glutamine synthetase (gs) in the mosquito, aedes aegypti, is induced in the midgut following a blood meal. mosquito gs message is detected as soon as 1 h post-blood feeding and remains stable for 18 h. using a pcr product encoding mosquito gs, a lambda gt10 adult female mosquito cdna library was screened. a cdna clone, pcl5a2, encoding the full translation product of mosquito gs was isolated and sequence analyses performed. mosquito gs cdna is 2.5 kb in length and its putative translation produc ... | 1998 | 9887510 |
| vitelline envelope genes of the yellow fever mosquito, aedes aegypti. | vitelline envelope genes from the mosquito aedes aegypti were analyzed with respect to their dna sequences, genomic representation, temporal and spatial expression profiles and response to 20-hydroxyecdysone. genomic clones of three vitelline envelope genes, 15a-1, 15a-2 and 15a-3 were isolated. southern analysis indicates that all three genes are represented by a single copy in the genome. the deduced amino acid sequences of all three vitelline envelope genes contain a conserved region of 46 re ... | 1998 | 9887508 |
| the anterior and posterior 'stomach' regions of larval aedes aegypti midgut: regional specialization of ion transport and stimulation by 5-hydroxytryptamine. | the 'stomach' region of the larval mosquito midgut is divided into histologically distinct anterior and posterior regions. anterior stomach perfused symmetrically with saline in vitro had an initial transepithelial potential (tep) of -66 mv (lumen negative) that decayed within 10-15 min to a steady-state tep near -10 mv that was maintained for at least 1 h. lumen-positive teps were never observed in the anterior stomach. the initial tep of the perfused posterior stomach was opposite in polarity, ... | 1999 | 9882637 |
| [natural features of oviposition of culex (melanoconion) group pilosus (diptera: culicidae)]. | eggs of culex (melanoconion) grupo pilosus were found in three oviposition traps set in the toilet of a bus station in joinville city, state of santa catarina, brazil, during a surveillance study of aedes aegypti. the intrinsic characteristic of group oviposition not directly on the water was confirmed. | 1998 | 9876429 |
| [pathogenic effect of the nematode parasite romanomermis iyengari(nematoda: mermithidae) in aedes aegypti mosquito larvae (diptera: culicidae) under laboratory conditions in the state of oaxaca, mexico]. | laboratory tests with waters from aedes aegypti linneaus (1762) breeding places were made to determine the pathogenic effect of the mermithid nematode romanomermis iyengari welch 1964 in mosquito larvae of this species. according to the results obtained, the administration of a dosage of 10:1 (10 preparasitics per mosquito larvae) showed levels of parasitism of 90, 93, 91, and 85% in mosquito larvae in the i, ii, iii, and iv stage, respectively. with the highest dosage of 20:1 (20 preparasitics ... | 1998 | 9842260 |
| larval rearing water and preexisting eggs influence oviposition by aedes aegypti and ae. albopictus (diptera: culicidae). | two-choice laboratory bioassays were used to evaluate oviposition responses of gravid aedes aegypti (l.) and ae. albopictus (skuse) to larval rearing water and eggs from prior egg deposition. oviposition responses by female ae. aegypti were similar between larval water from ae. aegypti and water controls but significantly greater to larval water from ae. albopictus (12.2%) compared with water controls. oviposition by ae. albopictus females increased significantly in response to larval water from ... | 1998 | 9835684 |
| mesocyclops longisetus effects on survivorship of aedes aegypti immature stages in car tyres. | the effect of the introduction of the entomophagous copepod mesocyclops longisetus (acuacultura f.c.b. strain) on the survival of aedes aegypti immature stages in car tyres was evaluated under semi-natural conditions in the municipality of merida, yucatan, mexico. life tables were constructed for the immature stages of the mosquito in the presence and absence of m. longisetus, and the survival data were compared using log-linear models. the data set was adjusted using the glim statistical packag ... | 1998 | 9824822 |
| presence of miniature inverted-repeat transposable elements (mites) in the genome of arabidopsis thaliana: characterisation of the emigrant family of elements. | although the genome of arabidopsis thaliana has a small amount of repetitive dna, it contains representatives of most classes of mobile elements. however, to date, no miniature inverted-repeat transposable element (mite) has been described in this plant. here, we describe a new family of repeated sequences that we have named emigrant, which are dispersed in the genome of arabidopsis and fulfil all the requirements of mites. these sequences are short, at-rich, have terminal inverted repeats (tirs ... | 1998 | 9807830 |
| biosynthesis of aedes aegypti lipophorin and gene expression of its apolipoproteins. | the biosynthesis of lipophorin of the yellow fever mosquito, aedes aegypti, was investigated. fat bodies were incubated in vitro with radiolabeled methionine and cysteine, and radiolabeled proteins secreted into the medium were analyzed by density gradient ultracentrifugation, sds-page and fluorography. lipophorin was synthesized in the fat body and secreted into the medium. its density was 1.114 g/ml, similar to that of lipophorin circulating in hemolymph. three peptides of a tryptic digest of ... | 1998 | 9807220 |
| dna binding and transactivation characteristics of the mosquito ecdysone receptor-ultraspiracle complex. | the steroid hormone 20-hydroxyecdysone is a key regulatory factor, controlling blood-meal triggered egg maturation in mosquitoes. to elucidate the ecdysone hierarchy governing this event, we cloned and characterized the ecdysone receptor (aaecr) and the nuclear receptor ultraspiracle (aausp), a retinoid x receptor homologue, from the mosquito, aedes aegypti, which form a functional complex capable of ligand and dna binding. here we analyzed the dna-binding properties of the aaecr.aausp heterodim ... | 1998 | 9765285 |
| estimation of predation by the larvae of toxorhynchites splendens on the aquatic stages of aedes aegypti. | predation by instars of toxorhynchites splendens on aquatic stages of aedes aegypti was studied by estimating functional response parameters such as attack rate (a') and handling time (th) in the laboratory. the predator displayed typical type-ii functional response, similar to that of most insect predators when presented with increasing densities of any given size class of prey. second instar predator attacked prey significantly at higher rate than the other instars. small prey were attacked at ... | 1998 | 9740296 |
| domestic aedes aegypti breeding site surveillance: limitations of remote sensing as a predictive surveillance tool. | this project tested aerial photography as a surveillance tool in identifying residential premises at high risk of aedes aegypti breeding by extending the use of a recently developed, ground-based, rapid assessment technique, the modified premise condition index (pci2). during 1995, we inspected 360 premises in townsville, australia for ae. aegypti breeding, and pci2 scores were recorded. the pci2 values were also estimated from 1:3,000 color and infrared aerial photograph interpretation for the ... | 1998 | 9715943 |
| aedes aegypti (diptera: culicidae) movement influenced by availability of oviposition sites. | marked aedes aegypti (l.) (5-6 d old) were released inside 2 groups of 5 houses (100 females per house) in a residential community in florida, pr, to compare behavior of gravid females at sites where oviposition containers were absent to sites where containers were abundant (i.e., 2 tires and 10 ovipots were added to each yard). two sequential releases were made so that both groups of houses were evaluated with oviposition containers removed and added. mosquitoes resting inside the 10 release ho ... | 1998 | 9701948 |
| isolation and characterization of the gene encoding a novel factor xa-directed anticoagulant from the yellow fever mosquito, aedes aegypti. | mosquito salivary glands secrete a number of proteins that inhibit mammalian hemostasis and facilitate blood feeding. we have isolated the protein product and corresponding cdna of a gene designated anticoagulant-factor xa (afxa), that encodes the factor xa (fxa)-directed anticoagulant of the yellow fever mosquito, aedes aegypti. the protein was purified partially by cation exchange chromatography and shown by enzyme activity profiles and sds-polyacrylamide gel electrophoresis analysis to have a ... | 1998 | 9694825 |
| effectiveness of ammonium solution in relieving type i mosquito bite symptoms: a double-blind, placebo-controlled study. | a randomized, double-blind, placebo-controlled study was conducted to compare the efficacy of ammonium solution with placebo in relieving type i symptoms caused by aedes aegypti mosquitoes on 25 healthy subjects. each subject was bitten once under laboratory conditions by aedes aegypti mosquitoes on the volar aspect of each forearm, and then received the treatment with ammonium solution and placebo, respectively. compared with placebo, the ammonium solution significantly (p < 0.0001) decreased i ... | 1998 | 9689301 |
| evaluation of repellent activities of cymbopogon essential oils against mosquito vectors of malaria, filariasis and dengue fever in india. | essential oils of four species and two hybrid varieties of cymbopogon grasses were evaluated for their repellent properties against the major vector mosquitoes, namely, anopheles stephensi, culex quinque-fasciatus and aedes aegypti, both in laboratory and field. the magnitude of repellency in the cymbopogon essential oils was found to be of moderate to high order. all grass species protected completely from mosquito bites for 4 hrs, whereas c. nardus provided protection for as much as 8-10 hrs o ... | 1998 | 23195906 |
| north carolina mosquito records. i. uncommon aedes and anopheles (diptera: culicidae). | new distribution records are provided for 10 mosquito species that are rare or uncommon in north carolina: aedes aegypti, ae. cinereus, ae. dupreei, ae. fulvus pallens, ae. hendersoni, ae. mitchellae, ae. thibaulti, ae. tormentor, ae. trivittatus, and anopheles atropos. biological notes are provided for habitats, behavior, and, in some cases, color patterns. comments are also made about 6 additional species that are rare or uncommon in north carolina. | 1998 | 9673917 |
| toxicity of a phenyl pyrazole insecticide, fipronil, to mosquito and chironomid midge larvae in the laboratory. | toxicity of a phenyl pyrazole insecticide, fipronil, to 4th-instar larvae of 6 species of colonized mosquitoes (aedes aegypti, ae. albopictus, ae. taeniorhynchus, anopheles quadrimaculatus, culex nigripalpus, and cx. quinquefasciatus) and 2 species of field-collected chironomid midges (chironomus crassicaudatus and glyptotendipes paripes) was evaluated in the laboratory. all mosquito species were highly susceptible with 48-h median lethal concentration (lc50) values ranging from 0.00043 ppm (ae. ... | 1998 | 9673927 |
| isolation and sequence of a partial vitellogenin cdna from the cockroach, blattella germanica (l.) (dictyoptera, blattellidae), and characterization of the vitellogenin gene expression. | a partial cdna clone of the vitellogenin gene from the cockroach blattella germanica has been isolated from a cdna expression library using an anti-vitellin-vitellogenin antiserum probe. the analysis of cdna inserts gave a sequence of 2,645 nucleotides corresponding to the 3' region. the deduced amino acid sequence is 825 residues long and is similar to the homologous portion of the vitellogenin of other insect species, especially that of the mosquito aedes aegypti. rna hybridization studies ind ... | 1998 | 9658559 |
| structural, genomic, and phylogenetic analysis of lian, a novel family of non-ltr retrotransposons in the yellow fever mosquito, aedes aegypti. | a retrotransposon named lian-aa1 was discovered in an intron of an aahr3-1 gene of the yellow fever mosquito, aedes aegypti. this retrotransposon contained a long open reading frame with 1,219 amino acids that included endonuclease, reverse transcriptase, and rnase h domains. it was shown that in the rock strain of ae. aegypti, there were up to 1,380 copies of lian elements, equivalent to 0.8% of the entire genome. five additional copies of lian elements were isolated, mapped by restriction dige ... | 1998 | 9656485 |
| laboratory evaluation of the biocontrol potential of mesocyclops thermocyclopoides (copepoda: cyclopidae) against mosquito larvae. | biocontrol potential of mesocyclops thermocyclopoides against first instar larvae of anopheles stephensi, aedes aegypti and culex quinquefasciatus was studied under laboratory conditions. it was found that m. thermocyclopoides had the highest predation efficacy against ae. aegypti followed by an. stephensi and cx. quinquefasciatus. there was a significant reduction in the predation efficacy of m. thermocyclopoides against cx. quinquefasciatus in the presence of alternate food (p < 0.01). the cag ... | 1997 | 9656415 |
| identification and cdna cloning of novel juvenile hormone responsive genes from fat body of the bean bug, riptortus clavatus by mrna differential display. | juvenile hormone (jh) induces termination of diapause and initiation of reproductive maturation in the adult female bean bug, riportus clavatus. applying pcr-based differential display, we have identified four novel jh-responsive cdnas, that is, three repressible (jr-1, 2 and 3) and one inducible (ji-1). these dna fragments were partially sequenced and compared with sequences in the data base. jr-3 was shown to have similarity to the transferrins of other insects, which have been reported as jh- ... | 1998 | 9654740 |
| mediation of deet repellency in mosquitoes (diptera: culicidae) by species, age, and parity. | laboratory bioassays assessed differences in the protection time provided by the repellent deet (n,n-diethyl-3-methylbenzamide) against 5-d-old nulliparous and 10-, 15-, and 20-d-old nulliparous and parous female aedes aegypti (l.), anopheles albimanus (weidemann), and anopheles quadrimaculatus say sensu lato. mean protection time was shortest against an. albimanus (1.6 h) and an. quadrimaculatus (1.5 h) and longest against ae. aegypti (6.5 h), but was not significantly influenced by mosquito ag ... | 1998 | 9615557 |
| dose-dependent effects of crf-like diuretic peptide on transcellular and paracellular transport pathways. | the mechanism of action of synthetic culex corticotropin-releasing factor (crf)-like diuretic peptide (ccrf-dp) was investigated in isolated, perfused malpighian tubules of the yellow fever mosquito, aedes aegypti. low concentrations of ccrf-dp (10(-10) and 10(-9) m) caused depolarizing oscillations of the lumen-positive transepithelial voltage (vt) in malpighian tubules, whereas high concentrations (10(-8) and 10(-7) m) first depolarized and then transiently hyperpolarized vt; ccrf-dp always lo ... | 1998 | 9612320 |
| nucleotide sequence and deduced amino acid sequence of a putative asparagine synthetase in the mosquito aedes aegypti (l.). | a cdna was cloned from a aedes aegypti head cdna library, containing the complete coding sequence for an asparagine synthetase homolog. the predicted polypeptide sequence exhibits high homology with different proteins of the 'purf' glutamine amidotransferase enzyme family. the aminoterminal region, containing cys-1 which is crucial to perform the glutaminase reaction, was highly conserved among the asparagine synthetase family. subsequent expression of the cdna yielded a 54,000 da protein corres ... | 1998 | 9602118 |
| the lethal effects of cyperus iria on aedes aegypti. | the sedge cyperus iria, a common weed in rice, contains large amounts of the insect hormone (10r) juvenile hormone iii (jh iii). given its widespread distribution in asia and africa, we examined the possibility that c. iria could be used as a safe, inexpensive, and readily available mosquito larvicide. plants of varying ages were harvested and leaves tested for lethal effects on larvae of the yellow fever mosquito, aedes aegypti. the median lethal doses (ld50s) for frozen leaves from 1- and 2-mo ... | 1998 | 9599328 |
| natural habitats of aedes aegypti in the caribbean--a review. | natural breeding habitats of aedes aegypti in the caribbean region were reviewed by conducting larval surveys in trinidad. puerto rico, and the u.s. virgin islands and referring to records from the mosquitoes of middle america project. twelve types of natural habitats were recorded: rock holes (9.7%), calabashes (2.4%), tree holes (19.5%), leaf axils (4.8%), bamboo joints (14.9%), papaya stumps (7.3%), coconut shells (4.8%), bromeliads (7.3%), ground pools (14.9%), coral rock holes (9.7%), crab ... | 1998 | 9599318 |
| the concentration-dependence of crf-like diuretic peptide: mechanisms of action. | the mechanism of action of synthetic ccrf-dp, the corticotropin-releasing factor (crf)-related diuretic peptide of the salt marsh mosquito culex salinarius, was investigated in isolated malpighian tubules of the yellow fever mosquito aedes aegypti. a low concentration of ccrf-dp (10(-9)mol l-1) caused a small but insignificant increase in transepithelial secretion of nacl and fluid, but significantly reduced transepithelial voltage and resistance without a change in short-circuit current, pointi ... | 1998 | 9576886 |
| cloning of cdna for vitellogenin of the parasitoid wasp, pimpla nipponica (hymenoptera: apocrita: ichneumonidae): vitellogenin primary structure and evolutionary considerations. | the cdna for vitellogenin (vg) of the parasitoid wasp pimpla nipponica (hymenoptera: apocrita) was cloned and sequenced. the deduced amino acid sequence with 1807 residues was obtained. the n-terminal 20 amino acids chemically determined for vitellin (vn) agreed completely with the deduced 20 amino acids that follow the 16 amino acid residues for putative signal peptide. the cdna clone for the vg of the turnip sawfly athalia rosae (hymenoptera: symphyta), previously obtained and partially sequen ... | 1997 | 9569645 |
| transient expression of the drosophila melanogaster cinnabar gene rescues eye color in the white eye (we) strain of aedes aegypti. | the lack of eye pigment in the aedes aegypti we (white eye) colony was confirmed to be due to a mutation in the kynurenine hydroxylase gene, which catalyzes one of the steps in the metabolic synthesis of ommochrome eye pigments. partial restoration of eye color (orange to red phenotype) in pupae and adults occurred in both sexes when first or second instar larvae were reared in water containing 3-hydroxykynurenine, the metabolic product of the enzyme kynurenine hydroxylase. no eye color restorat ... | 1997 | 9569641 |
| [dengue, arbovirus and migrations in the indian ocean]. | an arbovirus requires a competent haematophagous arthropod for transmission from one vertebrate to another susceptible host. air transportations allow quick transfers from place to place and the diffusion of potential vectors or infectious hosts. the merchandise transportation by containers allowed the diffusion of ae. albopictus from asia through africa and america. emerging arbovirusis may occur in zones where they do not exist. dengue which originally was located in southeastern asia, is the ... | 1998 | 9559164 |
| aedes aegypti survival and dispersal estimated by mark-release-recapture in northern australia. | the survival and dispersal of adult aedes aegypti were estimated in northern australia where sporadic outbreaks of ae. aegypti-borne dengue viruses have occurred in recent years. standard mark-release-recapture methods were used. in addition, a new sticky trap was used to capture the mosquitoes. prior to the field study, the survival and effect of marking ae. aegypti with fluorescent powder were determined in the laboratory. mortality was age-dependent and the marked cohorts had higher survival ... | 1998 | 9546403 |
| serum composition of aedes aegypti (diptera: culicidae) larvae and the production of an oviposition repellent are influenced by infection with the entomopathogenic digenean plagiorchis elegans (trematoda: plagiorchiidae), starvation, and crowding. | subjecting aedes aegypti (l.) larvae to conditions that induced the production of oviposition repellency also reduced their wet and dry weights and the concentration of total serum carbohydrates, amino acids, and proteins. thus, infection with metacercariae of the entomopathogenic digenean plagiorchis elegans (rudolphi), starvation for 7 d, or crowding for 2 d reduced larval dry weights by as much as 32, 20, and 23%, respectively, and wet weights by 20, 14, and 11%, respectively. total serum car ... | 1998 | 9538578 |
| expression and rapid purification of an aedes aegypti salivary allergen by a baculovirus system. | mosquito salivary proteins cause allergic reactions in humans. recombinant salivary allergens will facilitate both the diagnosis and immunotherapy of mosquito allergy. the aed a 1, a 68-kd apyrase in the saliva of aedes aegypti, has been demonstrated to be an allergen which binds to the ige of mosquito-allergic subjects. the baculovirus expression vector pbluebachis c equipped with an n-terminal histidine tag was used to express the aed a 1 protein. the cdna coding for the 3' significant portion ... | 1998 | 9531167 |
| identification of a steroidogenic neurohormone in female mosquitoes. | in the female mosquito, aedes aegypti, neurohormones are released from the brain in response to a blood meal and stimulate the ovaries to secrete ecdysteroid hormones, which modulate yolk protein synthesis in the fat body. neuropeptides with this bioactivity were isolated from head extracts, and partial sequences from these peptides when aligned gave a 31-residue sequence at the amino terminus. oligonucleotide primers for this sequence were used to amplify with the polymerase chain reaction a ge ... | 1998 | 9461584 |
| effect of heavy metals on aedes aegypti (diptera: culicidae) larvae. | studies were conducted to determine the biological effects of heavy metals on the development of aedes aegypti. embryos immersed in 32 ppm cu or 5 ppm cd did not hatch. the arrest of hatching was in part reversible by removal of the heavy metals. the mortality rate of third-instar larvae exposed to heavy metals for 24 h was metal and dose dependent; the 50% lethal concentration (lc50) endpoints were 3.1, 16.5, and 33 ppm for hg, cd, and cu, respectively. interestingly, a proportion of aedes aegy ... | 1998 | 9515074 |
| preliminary field evaluation of the combined adulticidal, larvicidal, and wall residual activity of ulv-applied bifenthrin against mosquitoes. | the combined adulticidal, larvicidal, and wall residual activity of ulv-applied bifenthrin, a synthetic pyrethroid, was evaluated in houses in kuala lumpur, malaysia, against larvae and adults of lab-bred aedes aegypti and culex quinquefasciatus. a portable ulv sprayer was used to disperse a ulv formulation of bifenthrin at a discharge rate of 45 ml/min. the results indicated that bifenthrin sprayed at this rate exhibited all the three activities against the test mosquitoes. complete adult morta ... | 1997 | 9491365 |
| transamination of 3-hydroxykynurenine to produce xanthurenic acid: a major branch pathway of tryptophan metabolism in the mosquito, aedes aegypti, during larval development. | an electrochemically active compound was detected in the larvae of aedes aegypti mosquitoes and progressive accumulation of this compound was observed during larval development. the compound was purified from mosquito larvae using various chromatographic techniques and spectral analysis of the purified compound resulted in its identification as xanthurenic acid. production of xanthurenic acid results from the transamination of 3-hydroxykynuorenine, and analysis of the biochemical pathway in xant ... | 1997 | 9474782 |
| an equipment for the study of behavioural responses of mosquitoes to residual application of synthetic insecticides. | excito-repellency (e-r) test box was developed to study under laboratory conditions the refractory behaviour of mosquitoes to residual application of synthetic insecticides. the observations obtained through the equipment were compared with another behavioural study of mosquitoes using standard who irritability test kit. during 16 hour exposure period there was restricted entry of mosquitoes in ddt treated chamber of e-r test box for all mosquito species tested (anopheles stephensi, aedes aegypt ... | 1997 | 9465527 |
| analysis of a ribosomal dna intergenic spacer region from the yellow fever mosquito, aedes aegypti. | we have sequenced the 1.8 kb intergenic spacer (igs) region from an aedes aegypti ribosomal dna repeat and have identified conserved functional motifs shared with the related mosquito, aedes albopictus. despite the shorter length and greater homogeneity of the ae. aegypti igs region, the sequences of two potential rna polymerase i core promoters and closely associated terminator elements were highly conserved. primer extension analysis indicated that the predominant transcription initiation site ... | 1998 | 9459426 |
| the gecko: an environmentally friendly biological agent for mosquito control. | laboratory experiments with aedes aegypti mosquitoes investigated the effects of light, mosquito density and physiological state on predation rates by the australian gecko gehydra dubia and the exotic asian house gecko hemidactylus frenatus. for both gecko species a positive correlation was demonstrated between prey density and the predation rate. using ae.aegypti males and unfed females as prey in a terrarium (0.054 m3), consumption rates reached 76-108/day for g.dubia and 63-109/day for h.fren ... | 1997 | 9430109 |
| molecular mapping of insecticide resistance genes in the yellow fever mosquito (aedes aegypti). | several loci conferring insecticide resistance in the yellow fever mosquito (aedes aegypti) have previously been mapped by simple recombinational mapping. here we describe correlation of these resistance phenotypes with molecular gene probes for insecticide target sites by rflp mapping. the para sodium channel gene homologue and the gaba receptor gene resistance to dieldrin map to the same genome regions as the ddt/pyrethroid and cyclodiene resistance loci, respectively. although the acetylcholi ... | 2010 | 9419893 |
| isolation of the v-atpase a and c subunit cdnas from mosquito midgut and malpighian tubules. | using conserved amino acid sequences for the design of oligonucleotide primers, we isolated cdna clones for two subunits of the v-atpase from the midgut and malpighian tubules of aedes aegypti larvae. the 3.1 kb cdna of the a subunit of the peripheral catalytic v1 sector codes for a protein of 68.6 kda. the protein contains conserved motifs, including an atp/gtp binding site, found in all other a subunits. southern analysis using the a subunit as a probe suggests the presence of only a single co ... | 1998 | 9397516 |
| site-directed mutagenesis of an acetylcholinesterase gene from the yellow fever mosquito aedes aegypti confers insecticide insensitivity. | insecticide resistance is a serious problem facing the effective control of insect vectors of disease. insensitive acetylcholinesterase (ache) confers resistance to organophosphorus (op) and carbamate insecticides and is a widespread resistance mechanism in vector mosquitoes. although the point mutations that underlie ache insensitivity have been described from drosophila, the colorado potato beetle, and house flies, no resistance associated mutations have been documented from mosquitoes to date ... | 1997 | 9371089 |
| selected lines of aedes aegypti with persistently distorted sex ratios. | a breeding scheme to isolate x chromosomes sensitive to drive by the t8 (trinidad) y chromosome of aedes aegypti (the md haplotype) is reported. crosses with an australian strain th.i (thursday island) revealed not only sensitive and resistant x chromosomes but also some with the capacity to drive against the t8 y chromosome. four strains were created in which sex ratio was male-distorted (28-36 per cent female) for 10 generations, with no regression towards sexual parity. the proportion of fema ... | 1997 | 9353868 |
| oviposition responses of aedes aegypti and ae. atropalpus (diptera: culicidae) females to waters from conspecific and heterospecific normal larvae and from larvae infected with plagiorchis elegans (trematoda: plagiorchiidae). | ovipositing aedes aegypti (l.) females were attracted to waters in which uninfected aedes atropalpus (coquillett) larvae had been reared, but were repelled by waters from larvae of either species infected with the entomopathogenic digenean plagiorchis elegans (rudolphi). in contrast, ae. atropalpus females were attracted to or repelled by waters from conspecific uninfected and infected larvae, respectively, but did not respond to corresponding waters from ae. aegypti larvae. the sensitivity of o ... | 1997 | 9379463 |
| isolation and properties of drosophila melanogaster ferritin--molecular cloning of a cdna that encodes one subunit, and localization of the gene on the third chromosome. | ferritin was purified from iron-fed drosophila melanogaster extracts by centrifugation in a gradient of potassium bromide. on polyacrylamide gel electrophoresis, the product showed two protein bands corresponding to the ferritin monomer and dimer. electrophoresis following dissociation with sds and 2-mercaptoethanol revealed three strong bands of approximately 25, 26, and 28 kda. n-terminal amino acid sequences were identical for the 25-kda and 26-kda subunits, but different for the 28-kda subun ... | 1997 | 9266686 |
| effect of ebastine on mosquito bites. | mosquito bites usually cause wealing and delayed bite papules. cetirizine decreases wealing, bite papules and pruritus but the effect of other antihistamines on mosquito bites is unknown. we studied the effect of ebastine in 30 mosquito bite-sensitive adult subjects. ebastine 10 mg or 20 mg and placebo were given for 4 days in a cross-over fashion. aedes aegypti bites were given on forearms. the size of the bite lesions and pruritus (visual analogue score) were measured at 15 min, 2, 6, and 24 h ... | 1997 | 9228228 |
| reduced transfer of male accessory gland proteins and monandry in female aedes aegypti mosquitoes. | we examined whether female aedes aegypti (l.) mosquitoes would remate after they first mated with a male that was reared on a suboptimal larval diet and that as a result, transferred reduced amounts of male accessory gland proteins. accessory gland proteins from males labeled with 3h leucine were not detected in females allowed to male mate with the labeled males after they first mated with either low diet or high diet males. the amount of the male accessory gland protein transferred by smaller, ... | 1997 | 9221745 |
| zebedee: a novel copia-ty1 family of transposable elements in the genome of the medically important mosquito aedes aegypti. | we have utilised pcr to directly identify a novel family of copia-ty1 retrotransposable elements (rtps) in the genome of the mosquito aedes aegypti. two members of the family have been sequenced in their entirety and their structural characteristics determined. zebedeei is 3505 bp long and appears to be flanked by 21bp direct repeat sequences. a single open reading frame (orf) of 972 amino acids has the coding potential for a polyprotein with homology corresponding to the conserved amino acid mo ... | 1997 | 9197409 |
| transposition of the hermes element in embryos of the vector mosquito, aedes aegypti. | using a plasmid-based transpositional recombination assay in vivo, we have demonstrated that hermes, a short inverted repeat type transposable element from musca domestica, can transpose in aedes aegypti embryos. hermes transpositions in ae. aegypti have all the characteristics observed during hermes transposition in its host m. domestica and in related species. these characteristics include an absolute dependence on the expression of the hermes transposase and a preference for the integration s ... | 1997 | 9219363 |
| molecular characterization of a defensin gene from the mosquito, aedes aegypti. | insect immune proteins, defensins, are inducible anti-gram-positive bacterial peptides. we report here the identification of two defensin genes from the mosquito, aedes aegypti, which encode a large 541 bp transcript (aadef ala) and a small 473 bp transcript (aadef asm). the cdna corresponding to aadef ala was cloned, sequenced, and compared with the previously reported aadef asm cdna. the aadef ala gene was isolated through genomic library screening and characterized. it putative regulatory reg ... | 1997 | 9219362 |
| fish landmarks for aedes aegypti chromosomes. | aedes aegypti metaphase chromosome landmarks have been developed so that each chromosome of the haploid genome can be unambiguously identified and oriented by fluorescence in situ hybridization (fish) and digital imaging microscopy. the fish tags were derived from three cosmids that contain repetitive ae. aegypti sequences and their unique fish tagging characteristics are demonstrated. the sequence of the three chromosomal tags revealed that the chromosome 1 tag is an 18s fragment from the ribos ... | 1997 | 9099584 |
| insulin-mediated secretion of ecdysteroids from mosquito ovaries and molecular cloning of the insulin receptor homologue from ovaries of bloodfed aedes aegypti. | the reproductive cycle of female mosquitoes is activated by ingestion of blood from vertebrate hosts. shortly after feeding, neurohormones are released from the brain neurosecretory system and stimulate the ovaries to secrete ecdysteroids, which are necessary for vitellogenesis by the fat body. because bombyxins, which are insulin-like peptides, stimulate ecdysteriodgenesis in silkworm larvae, we tested porcine insulin and found that it activates ecdysteroidogenesis and protein synthesis in ovar ... | 1997 | 9099579 |
| a single cdna encodes all three aedes leucokinins, which stimulate both fluid secretion by the malpighian tubules and hindgut contractions. | a cdna encoding preproleucokinin was isolated from a cdna library of the mosquito aedes aegypti. the deduced amino acid sequence of aedes preproleucokinin contains a putative signal peptide of 18 amino acid residues and a 210-amino acid residue proleucokinin. within the proleucokinin are encoded one copy each of the aedes leucokinins 1, 2, and 3 isolated previously from this species (veenstra, j. a. (1994) biochem. biophys. res. commun. 202, 715-719). all three aedes leucokinins depolarize the t ... | 1997 | 9099680 |
| inheritance of larval resistance to permethrin in aedes aegypti and association with sex ratio distortion and life history variation. | the genetic mechanisms that confer larval permethrin resistance were investigated in two strains of aedes aegypti, vectors of yellow fever and dengue hemorrhagic fever. larval resistance to permethrin in an ae. aegypti field-collected resistant couva (r) strain was associated with the sex-determining locus by analysis of backcrosses to the susceptible rockefeller (s) strain. the median lethal concentrations (lc50s) of these strains were 23.1 (95% confidence interval = 22.0-24.3) and 2.2 (2.0-2.3 ... | 1997 | 9158058 |
| cdna cloning and pattern of expression of an adult, female-specific chymotrypsin from aedes aegypti midgut. | a cdna for a midgut chymotrypsin, induced by a blood meal, has been cloned and sequenced from the mosquito aedes aegypti. the 938 base sequence codes for a 268 amino acid protein, which contains an 18-residue signal peptide and a seven-residue activation peptide. the deduced amino acid sequence contains several features typical of chymotrypsin proteases, including the catalytic triad of serine proteases and the residues that determine the chymotrypsin substrate specificity pocket. the chymotryps ... | 1997 | 9134710 |
| aedes aegypti surveillance and correlation with the occurrence of dengue fever in bahia, brazil. | due to the high frequency of dengue fever cases and the presumed association of such an epidemic with an increase in the population of the mosquito vector, aedes aegypti, we examined the records of the ministry of health in the state of bahia, brazil, regarding the monitoring of domestic mosquito larvae in municipalities throughout the state. the "house index" number for larvae in domestic water reservoirs was determined for each municipality based on annual surveys from 1990 to 1994, and in 199 ... | 1997 | 11107237 |
| effects of two insect growth regulators on the susceptibility of aedes aegypti (diptera:culicidae) to molinema dessetae (nematoda:filarioidea). | the effects of 2 growth regulators, diflubenzuron (dfb) and oms 2017, on the ability of females aedes aegypti to become infected with molinema dessetae was studied under laboratory conditions. oms 2017 and dfb had no effect on either the amount of blood ingested or the microfilarial load. the infective potential of females that survived dfb treatment was significantly greater than untreated females, but there was no difference between oms 2017-treated and control females. the percentage of infec ... | 1997 | 9152874 |
| methoprene affects the rotation of the male terminalia of aedes aegypti mosquitoes. | the rotation of the male terminalia of aedes aegypti mosquitoes was investigated with chemical surgical, and mechanical methods. neither decapitation nor ventral nerve cord transection affected the postemergence rotation, but methoprene administered to 4th-instar larvae and early instar pupae slowed rotation significantly and, in the case of pupal treatment, also interfered with successful insemination. methoprene appeared to interfere with the developmental maturation of the cross muscles locat ... | 1997 | 9152867 |
| biochemical, molecular, and phylogenetic analysis of pyruvate carboxylase in the yellow fever mosquito, aedes aegypti. | pyruvate carboxylase (pc, pyruvate: carbon dioxide ligase [adp-forming], ec 6.4.1.1) was purified from the yellow fever mosquito, aedes aegypti. the purified pc showed two polypeptides of similar m(r) (133 and 128 k). the n-terminal sequences of both polypeptides were shown to be very similar, if not identical. a polyclonal antiserum against the 133 kda polypeptide cross-reacted strongly with the 128 kda polypeptide. pc was found in all tissues examined. using a semi-quantitative western blot as ... | 1997 | 9066123 |
| juvenile hormone controls early trypsin gene transcription in the midgut of aedes aegypti. | early trypsin is a female-specific protease present in the aedes aegypti midgut during the first few hours after ingestion of a blood meal. the enzymatic activity of early trypsin plays an essential role in the transcriptional activation of the late trypsin gene, which encodes the major midgut endoprotease involved in blood meal protein digestion. transcription of the early trypsin gene is part of the normal post-emergence maturation of the midgut in the adult female. abdominal ligation within 1 ... | 1997 | 9013256 |
| identification of three allatostatins and their cdna from the mosquito aedes aegypti. | three allatostatins have been isolated from the mosquito aedes aegypti. these peptides have the following structures: ser-pro-lys-tyr-asn-phc-gly-leu-amide, leu-pro-his-tyr-asn-phe-gly-leu-amide, and arg-val-tyr-asp-phe-gly-leu-amide. a cdna encoding these peptides was isolated from an abdominal ganglia cdna library and sequenced. it was found to encode two additional allatostatins: ala-ser-ala-tyr-arg-tyr-his-phe-gly-leu-amide and leu-pro-asn-arg-tyr-asn-phe-gly-leu-amide. northern analysis of ... | 1997 | 9357049 |
| structure of the gypsy moth vitellogenin gene. | genomic clones containing the vitellogenin (vg) gene from the gypsy moth were isolated from two genomic libraries and characterized. the nucleotide sequence of a 16,132 bp region of the gypsy moth genome was determined which included a 3,666 bp region upstream from the transcription initiation site and 499 bp region downstream from the transcribed region. primer extension analysis was performed to identify the transcription initiation site. gene sequence confirmed the sequence of vgmrna recently ... | 1997 | 9327584 |
| central projections of the maxillary and antennal nerves in the mosquito aedes aegypti | in the mosquito aedes aegypti, co2-sensitive receptor neurones are located together with two other types of chemoreceptor neurones in club-shaped sensilla basiconica on the most distal segment of the maxillary palps. in order to identify the central target neuropiles of these neurones and to determine whether antennal receptor neurones project into the same area, the palpal and antennal nerves were labelled by anterograde staining with horseradish peroxidase and by experimentally induced degener ... | 1997 | 9319784 |
| the feasibility of eradicating aedes aegypti in the americas. | | 1997 | 9128110 |
| lipophorin levels in the yellow fever mosquito, aedes aegypti, and the effect of feeding. | high density lipophorin (hdlp) is the major lipid transport vehicle in insect hemolymph. using an indirect elisa, levels of hdlp were measured in the yellow fever mosquito, aedes aegypti. the level of lipophorin, when normalized to the total weight of the insect, was similar in the different developmental stages. starvation (access to water only) of adult females did not affect the level of hdlp nor its density when compared to sugar-fed females. on the other hand, blood feeding (of normally sug ... | 1997 | 9055439 |
| muscle actin gene from aedes aegypti (diptera:culicidae). | a recombinant phagemid containing a 1,240-bp insert encoding an actin was isolated from a yellow fever mosquito, aedes aegypti (l.), complementary dna library. this insert (pbs-act35) contained an open reading frame of 822 bp whose deduced amino acid sequence exhibited > 95% homology with the carboxyl terminal 274 amino acids of drosophila melanogaster meigen and silkworm, bombyx mori (l.), actin genes. reverse transcriptase-polymerase chain reaction was used to clone and determine the sequence ... | 1996 | 8961646 |
| insecticide impregnated cotton fabrics of different hydrophobicity against aedes aegypti (diptera:culicidae). | residual efficacy of synthetic pyrethroids, viz, permethrin, deltamethrin, lambdacyhalothrin and an insect repellent depa in cotton fabrics of different hydrophobicity was tested against aedes aegypti. amino silicone was used for enhancing the hydrophobicity of the fabrics. the results showed that there was an increasing trend in repellency/feeding deterrency with the increase in hydrophobicity up to 17.5 weeks at an optimum level of 30g/l. the adulticidal effect lasted for 1 to 4 weeks and this ... | 1996 | 9185280 |
| discovery of aedes albopictus in guatemala. | aedes albopictus was discovered for the first time in guatemala during june 1995. it was collected in 10 out of 15 localities sampled in and around the community of puerto barrios in the department of izabal. the location of the positive collections indicates a more rural distribution than aedes aegypti. sampling was conducted along the atlantic highway up to the capital city to determine the extent of its introduction into the country. larvae were not found more than 4 km outside of puerto barr ... | 1996 | 8887235 |
| enzymatic variability in natural populations of aedes aegypti (diptera:culicidae) from brazil. | yellowfever mosquitoes, aedes aegypti (l), were collected from 3 towns located within 70 km of each other in the state of sao paulo to evaluate the protein variability of natural populations. electrophoretic analysis permitted the identification of 6 loci responsible for the production of leucine aminopeptidase, 3 for esterases and malic enzyme, 2 for malate dehydrogenase, isocitrate dehydrogenase and alpha-glycerophosphate dehydrogenase, and 1 for phosphoglucomutase. for the 3 populations, the ... | 1996 | 8840678 |
| the mosquito ultraspiracle homologue, a partner of ecdysteroid receptor heterodimer: cloning and characterization of isoforms expressed during vitellogenesis. | we report the cloning and characterization of two isoforms of the ultraspiracle homologue (aausp) from the mosquito, aedes aegypti. the 2.33-kb aauspa cdna has an open reading frame (orf) of 484 amino acids encoding a polypeptide of 54 kda, whereas the 2.14-kb aauspb orf of 459 amino acids encodes a 51.3 kda polypeptide. the aauspa and aauspb proteins differ only in the n-terminal portion of the variable a/b domain. the aausp dna-binding domain shares 92% and 97% identities with the respective d ... | 1996 | 8892313 |
| cloning and expression analysis of aedes aegypti opsin: adaptation of an in situ hybridization protocol for mosquitoes. | opsin is a g protein coupled photoreceptor that activates a signal transduction cascade in the ommatidia. its primary and secondary structure is conserved both in insects and vertebrates as exemplified by the drosophila opsins. through serendiplious cloning of a pcr fragment, we have identified an opsin cdna. the latter was used to clone full length cdnas from a mosquito head library. the main purpose of cloning was to have a positive control probe to establish an in situ hybridization protocol ... | 1996 | 8799735 |
| adaptive larval thermotolerance and induced cross-tolerance to propoxur insecticide in mosquitoes anopheles stephensi and aedes aegypti. | fourth-instar larvae of mosquitoes anopheles stephensi and aedes aegypti normally died within 90 min at 43 degrees c. pre-exposure to high but sublethal temperatures conferred adaptive thermotolerance, dependent on the temperature and the duration of pre-exposure. adaptive cross-tolerance to propoxur (a carbamate insecticide) was also induced in larvae by pre-exposing them to sublethal temperatures. pre-exposure to sublethal concentrations of propoxur was found to confer cross-thermotolerance to ... | 1996 | 8887340 |
| yellowfever mosquito (diptera:culicidae) introduced into landi kotal, pakistan, by tire importation. | although the recent distribution of yellowfever mosquito, aedes aegypti (l.), in pakistan has been restricted to the port city of karachi, adult and immature mosquitoes breeding in imported tires in warehouses at landi kotal (north-west frontier province) were identified as ae. aegypti. the patterns of tire trade and the current disjunct distribution of ae. aegypti indicated that the introduction into landi kotal may have been either from karachi or india. thermal fog application of pirimiphos-m ... | 1996 | 8699469 |
| response of anopheles farauti to permethrin-treated net and cloth fabrics in the laboratory. | the median effective dose of permethrin for knockdown and mortality of anopheles farauti s. s. exposed to 2 net and 2 cloth fabrics was estimated. there were no significant differences in the knockdown or mortality of an. farauti or aedes aegypti following 15-180-sec exposure to permethrin-treated bed nets and polyester/cotton fabric. despite the presence of permethrin (0.007-0.068 mg/cm2) in bed net material, 18.2-50% of an. farauti adults successfully obtained a blood meal through the nets, an ... | 1996 | 8827613 |
| effect of cetirizine on the inflammatory cells in mosquito bites. | mosquito bites frequently cause wealing and delayed papules which appear within a few hours after the bites and may persist for several days. cetirizine is an effective drug against mosquito bites by decreasing wealing and also the size and pruritus of the delayed bite papules. | 1996 | 8809428 |
| mosquito dopa decarboxylase cdna characterization and blood-meal-induced ovarian expression. | dopa decarboxylase (ddc) functions in insect catecholamine biochemistry to produce materials essential for cross-linking reactions that result in tanning and/or melanization, include tanning of the mosquito egg chorion and encapsulation of parasites. we have cloned ddc from the mosquito, aedes aegypti, and studied its expression in response to blood-feeding, which initiates events necessary for egg maturation in mosquitoes. the ae. aegypti ddc cdna was isolated via heterologous screening using a ... | 1996 | 8673262 |
| critical examination of aedes aegypti indices: correlations with abundance. | the following immature stage indices for aedes (stegomyia) aegypti surveillance were evaluated in four north queensland, australia towns with respect to their relationship to immature and adult female densities: breteau, house, container, larval density, stegomyia (and modifications thereof), and a newly created adult productivity index. spearman's correlations of indices that considered larval or immature (larvae and pupae) numbers had a better relationship with immature abundance but this was ... | 1996 | 8644913 |
| cloning and characterization of cdnas encoding the antibacterial peptide, defensin a, from the mosquito, aedes aegypti. | insect defensins are cationic, inducible antibacterial peptides. four full-length cdnas encoding defensin a from the mosquito aedes aegypti were cloned using polymerase chain reaction (pcr) and sequenced. all four cdnas are 473 base pairs long, bearing an open reading frame of 98 amino acids with a few substitutions in the signal peptide domain. the deduced amino acid sequence of aedes aegypti defensin (aadef) contains a signal peptide sequence of 18 amino acids followed by a 40-amino acid putat ... | 1996 | 8814787 |
| community involvement in the control of aedes aegypti. | in the past 15 years, there has been a dramatic resurgence of dengue and dengue hemorrhagic fever worldwide, with increased frequency of epidemics and geographic expansion of both the mosquito vectors and the viruses. the reasons for this resurgence are not well understood, but include uncontrolled and unplanned urbanization, increased movement of people and viruses by airplane and lack of effective control of aedes aegypti, the principal mosquito vector of dengue viruses. the recommended method ... | 1996 | 8740894 |
| fish digital imaging microscopy in mosquito genomics. | the yellow fever mosquito, aedes aegypti, transmits pathogens that affect both humans and livestock, and has been the focus of extensive research to identify genetic loci that may be useful in control strategies. fluorescence in situ hybridization (fish) and digital imaging microscopy have provided a rapid mechanism to populate the physical map with probes derived from genetic markers, cdnas and recombinant genomic libraries. when the physical and genetic linkage maps are aligned, map-based clon ... | 1996 | 15275237 |
| toxicity of insecticides to toxorhynchites splendens and three vector mosquitos and their sublethal effect on biocontrol potential of the predator. | toxicity of six larvicides ie fenthion, temephos, malathion, deltamethrin, alphamethrin (fendona), oms 3031 and five adulticides ie malathion, fenitrothion, propoxur, deltamethrin, permethrin to aedes aegypti, culex quiquefasciatus, anopheles stephensi and the predator, toxorhynchites splendens was studied for evaluating safety margin. concentrations of alphamethrin that killed 50% larvae of t. splendens were 53 and 12 times more than that which killed cx. quinquefasciatus and ae. aegypti. in ca ... | 1996 | 9031419 |
| involvement of peroxidase in chorion hardening in aedes aegypti. | peroxidase activity is detectable in aedes aegypti ovaries, containing developing eggs, at 24 h following blood feeding, and peak peroxidase activity is reached at 36-48 h after the blood-meal. peroxidase is associated with the chorion layer in mature eggs and the majority of the enzyme is released from the chorion layer by treating the isolated chorion fraction with sds/urea. analysis of the sds/urea solubilized chorion proteins using sds-page with tropolone/h2o2 or dopa staining verified the p ... | 1996 | 8900599 |
| purification and characterization of a major glutathione s-transferase from the mosquito anopheles dirus (species b). | the major form of glutathione s-transferase (gst) activity from the mosquito anopheles dirus (species b), a vector of malaria in thailand has been purified 421-fold. it constituted approx. 20% of the total measured cdnb conjugating activity in the homogenate. this enzyme appeared as a single band of 25.0 +/- 0.26 kda on sds-page and was kinetically characterized with 10 substrates and 4 inhibitors. the enzyme is capable of catalysing dehydrochlorination of 1,1,1-trichloro-2,2-bis-(p-chlorophenyl ... | 1996 | 8900597 |
| stable expression of insect gaba receptors in insect cell lines. promoters for efficient expression of drosophila and mosquito rdl gaba receptors in stably transformed mosquito cell lines. | we are interested in establishing stably transformed insect cell lines efficiently expressing the insect gamma-aminobutyric acid (gaba) receptor subunit gene resistance to dieldrin or rdl. in order to facilitate this we utilized a system based on stable transformation of aedes albopictus mosquito cell lines using the dihydrofolate reductase (dhfr) gene as a selected marker. here we report the production of stable mosquito cell lines carrying high copy numbers of rdl genes from both drosophila an ... | 1996 | 8601436 |
| functional analysis of a mosquito gamma-aminobutyric acid receptor gene promoter. | a single point mutation in the insect gamma-aminobutyric acid receptor (gabar)-encoding gene (rdl) confers high levels of resistance to cyclodienes in drosophila and other insects. we were interested in studying the promoter of this gene for two reasons. firstly, to define the elements underlying rdl expression. secondly, to identify the minimum set of regulatory elements necessary for construction of a functional rdl minigene. such an insecticide-resistance-associated minigene should form a str ... | 1996 | 8654932 |
| generation of expressed sequence tags and sequence-tagged sites as physical landmarks in the mosquito, aedes aegypti, genome. | twenty-three clones were developed as expressed sequence tags (ests) and sequence-tagged sites (stss) that provide broad coverage of the mosquito, aedes aegypti, genome at an average spacing of about 7.2 cm. each of these clones had been mapped previously as a restriction fragment length polymorphism (rflp) marker. nineteen of these clones represent anonymous cdnas and 4 clones represent known genes. forty-seven percent of the anonymous cdnas showed significant deduced amino acid sequence simila ... | 1996 | 8851807 |
| quiescent ovarioles in the mosquito aedes aegypti (l.) (diptera: culicidae). | | 1996 | 8729634 |
| ovulation and post-ovulation changes in the terminal region of the ovarioles of the mosquito aedes aegypti (l.) (diptera: culicidae). | detachment of the follicular epithelium from the chorion occurs prior to the passage of the mature egg into the calyx lumen, resulting in a mature oocyte lying free in an epithelial sac. the mature oocytes rupture the epithelial sac, including the tunica propria at the posterior pole, then break through the basal (or granular basal) bodies to enter the calyx lumen. just after ovulation, the formerly terminal parts of the ovariole remain attached to the posterior end of the egg sac near its openi ... | 1996 | 8729630 |
| leucokinin increases paracellular permeability in insect malpighian tubules | there are two major transport pathways across epithelia: a transcellular pathway through cells and a paracellular pathway between cells. previous electrophysiological studies in mosquito malpighian tubules suggested that the neuropeptide leucokinin-viii (lk-viii) increases the chloride permeability of the paracellular pathway. to test the effect of lk-viii on the paracellular pathway further, we measured transepithelial permeabilities of inulin and sucrose in isolated malpighian tubules from the ... | 1996 | 9320463 |
| [the prevention of dengue in guadeloupe]. | dengue is the only disease transmitted by mosquitoes in the guadeloupe islands of french antilles. two dengue outbreaks were reported in 1992 and 1994. aedes aegypti is recognized the only vector of dengue in guadeloupe, and the 200 l drums are the most important breeding-sites. vector control in guadeloupe includes community health education, larvicide and adulticide spraying, and the destruction of the breeding-sites. however, ae. aegypti control has limitations such as the lack of participati ... | 1996 | 8924773 |
| [oviposition and dispersion of aedes aegypti in an urban environment]. | it is generally accepted that female aedes aegypti do not fly more than 50-100 m in their entire lifetime, yet the rapidity with which this species colonizes new areas, and the explosive nature of dengue and yellow fever epidemics appear to contradict this. using molecular methods, we have confirmed that the ae. aegypti females lay small numbers of eggs at many sites. the distribution of available sites implies that the female may fly a considerable distance to deposit her whole egg batch. we de ... | 1996 | 8924769 |
| central olfactory pathways in mosquitoes and other insects. | studies of cns processing of olfactory information have contributed significantly to understanding olfactory-guided behaviour in insects. evidence in moths suggests that each glomerulus in the antennal lobes has a unique property: receptor-axon projections and dendritic arborizations of uniglomerular output neurons can relate to anatomically and functionally distinct glomeruli. similar correlations are not typical of the concentrically organized locust antennal lobes. insights about odour proces ... | 1996 | 8894298 |
| the premise condition index: a tool for streamlining surveys of aedes aegypti. | premise inspections and treatment of the larval habitats of container-breeding aedes aegypti are extremely labor intensive. since this means of control is the only one available in relation to dengue fever, this report presents an effective approach for streamlining premise surveys in north queensland, australia. from a survey of 877 premises in townsville, charters towers, and mingela/ravenswood in 1990, occupier and premise variables were collected to examine any relationships with the presenc ... | 1995 | 8561259 |