| proline can be utilized as an energy substrate during flight of aedes aegypti females. | in order to determine whether proline can be utilized as fuel during flight of aedes aegypti, proline, alanine, and glutamine concentrations were monitored at 0, 30 and 60 min after flight using sugar-fed males and females, and blood meal-fed females. in sugar-fed and blood meal-fed females, flight lead to a significant decrease in proline and a significant increase in glutamine concentration in both hemolymph and thorax. only during flight after a blood meal was a significant increase in the al ... | 2003 | 12804719 |
| insecticidal activity and chemical composition of volatile oils from hyptis martiusii benth. | the essential oils from leaves and inflorescences of hyptis martiusii benth were analyzed by gc-ms. twenty-six compounds representing 93.2% of the essential oil of leaves were characterized; delta-3-carene (22.5%), 1,8-cineole (24.27%), beta-caryophyllene (6.15%), and bicyclogermacrene (6.32%) were found as the major components. in the essential oil of inflorescences 27 compounds representing 87.7% of the oil were identified. the major components were delta-3-carene (13.5%), alpha-pinene (5.78%) ... | 2003 | 12797740 |
| effects of putative diuretic factors on intracellular second messenger levels in the malpighian tubules of aedes aegypti. | intracellular levels of the second messengers, 3',5'-cyclic adenosine monophosphate (camp) and inositol 1,4,5-trisphosphate (ip(3)) were measured in the malpighian tubules of aedes aegypti following the in vitro application of 5-hydroxytryptamine (5-ht) and the putative mosquito diuretic peptides, culex salinarius diuresin and mosquito leucokinins (culekinin depolarizing peptides (cdps) i, ii, iii, a. aegypti leucokinin peptides (alps) i, ii, iii). the c. salinarius diuresin significantly (p<0.0 ... | 1999 | 12770358 |
| the effect of putative diuretic factors on in vivo urine production in the mosquito, aedes aegypti. | changes in total urine production were measured in the mosquito, aedes aegypti, following injection of 5-hydroxytryptamine (5-ht), culex salinarius diuresin, culekinin depolarizing peptides (cdp-i, ii and iii) or the a. aegypti leucokinin peptides (alp-i, ii and iii). all stimulated total urine production in a dose dependent manner. 5-ht was the least potent in urine production experiments with ed(50) values nearly 100-fold higher than other diuretic agonists. doses greater than 2x10(-4) &mgr;mo ... | 1999 | 12770357 |
| sensitivity of the mosquito aedes aegypti (culicidae) labral apical chemoreceptors to phagostimulants. | the feeding of aedes aegypti (l.) on blood and nectar is induced by phagostimulants: adenine nucleotides and sugars respectively. this work examines the responses of the four chemoreceptor cells in the labral apical sensilla to these phagostimulants. the apical chemoreceptors can detect the presence of adenine nucleotides. this part of the response is in good agreement with the gorging behavior. the output of the chemoreceptors cannot distinguish among different adenine nucleotides or among thei ... | 1999 | 12770348 |
| a molecular view of trypsin synthesis in the midgut of aedes aegypti. | ingestion of a blood meal induces two phases of trypsin synthesis in the midgut of aedes aegypti females. the first phase, which encompasses the first 4-6 hours following a blood meal, is characterized by the presence of small amounts of early trypsin. the second phase, which occurs between 8 and 36 hours after blood feeding, is characterized by the presence of large amounts of late trypsin. a specific form of regulation of trypsin synthesis characterizes each phase: early trypsin synthesis is r ... | 1999 | 12770346 |
| sensitivity of the mosquito aedes aegypti (culicidae) labral apical chemoreceptors to blood plasma components. | the phagostimulants from the cellular fraction of blood induce gorging of aedes aegypti (l.), and this process is enhanced by some plasma components. this project examines the responses of the labral apical chemoreceptors to plasma components enhancing phagostimulation. from the electrophysiological responses of the labral apical chemoreceptors four cells were identified by the waveform of their action potentials. three of the cells (cell 2, cell 3 and cell 4) responded in a dose dependent manne ... | 1999 | 12770332 |
| localization of the mosquito insulin receptor homolog (mir) in reproducing yellow fever mosquitoes (aedes aegypti). | the female mosquito takes a blood meal to produce a batch of eggs. initiation of egg maturation and growth of oocytes is governed by several endocrine factors. peptide factors from the brain are involved in this process and some are also responsible for the induction of ecdysone secretion. the latter appears to be required to maintain a high rate of vitellogenin synthesis. by analogy with the known functions of insulin-like molecules (e.g. bombyxins) which in insects activate the secretion of ec ... | 1998 | 12770311 |
| factors modulating the blood feeding behavior and the electrophysiological responses of labral apical chemoreceptors to adenine nucleotides in the mosquito aedes aegypti (culicidae). | the feeding of aedes aegypti (l.) on blood is induced by the presence of phagostimulants: adenine nucleotides. three chemoreceptive cells in the labral apical sensilla can distinguish the presence of adenine nucleotides depending on the other stimulus components. this work aims at correlating the sensory information arising from the labral apical sensilla with the feeding behavior in response to the same stimuli. the saline stimulating solution, containing adenine nucleotides, is modulated by ch ... | 1999 | 12770292 |
| adaptation of aedes aegypti (diptera: culicidae) oviposition behavior in response to humidity and diet. | effects of humidity and sugar concentration on the fecundity, temporal oviposition patterns and survival of a tropical strain of aedes aegypti (l.) were investigated. fecundity was significantly reduced by low humidity, but was not affected by sugar concentration. low humidity caused a significant decrease in percentage survival after 19 days as compared to high humidity. oviposition was inhibited by host availability for eight successive days. when access to a host was no longer provided, ovipo ... | 1999 | 12770289 |
| intracellular and luminal ph measurements of malpighian tubules of the mosquito aedes aegypti: the effects of camp. | in order to understand the critical role that hydrogen ions play in fluid secretion in malpighian tubules, intracellular and luminal ph and k+ measurements were performed in isolated malpighian tubules of the yellow fever mosquito (aedes aegypti). the intracellular ph was 7.03+/-0.05 (n=15 malpighian tubules (mt)) and the luminal ph was 7.19+/-0.09 (n=99 mt) when bathed in saline at a ph of 7.0. the lumen potential is positive, thus net proton secretion into the lumen is active. the intracellula ... | 1999 | 12770272 |
| oscillations of voltage and resistance in malpighian tubules of aedes aegypti. | the transepithelial voltage (v(t)) of isolated malpighian tubules of the yellow fever mosquito aedes aegypti spontaneously oscillates in more than half the tubules. typically, v(t) decreases and then rises at a frequency of 2 oscillations/min with a duration of 16 s. in 6 isolated perfused tubules studied in detail, v(t) oscillates between 50.5 mv and 15.7 mv in parallel with (1) oscillations of the transepithelial resistance (r(t)) between 7.61 komegacm and 3.63 komegacm, (2) oscillations of th ... | 2000 | 12770237 |
| a temporal profile of the endocrine control of trypsin synthesis in the yellow fever mosquito, aedes aegypti. | trypsin synthesis and secretion is induced after the female mosquito takes a blood meal. its peak activity has been shown to be proportional to the amount and quality of food uptake. further regulatory elements, hormones from the brain and the ovary, increase the synthethic rate of trypsin in the midgut by a factor of two. we investigated the temporal effect of removing the humoral factors by decapitation and ovariectomy. trypsin synthesis was reduced to less than half its normal output when the ... | 1998 | 12770164 |
| midgut exopeptidase activities in aedes aegypti are induced by blood feeding. | midgut extracts from aedes aegypti females exhibited hydrolytic activities against synthetic substrates for carboxypeptidase a, carboxyopeptidase b and leucine-aminopeptidase. the three activities showed a broad ph optimum, with maximum activities at ph between 6.5 and 8.5. enzymatic activities were further characterized by testing the effects of a variety of protease inhibitors. captopril and 1-10-phenantroline inhibited the activities of carboxypeptidases a and b, while leuhistin, amastatin an ... | 2002 | 12770120 |
| membrane conductances of principal cells in malpighian tubules of aedes aegypti. | two-electrode voltage clamp (tevc) methods were used to explore conductive transport pathways in principal cells, the dominant cell type in malpighian tubules of the yellow fever mosquito. the basolateral membrane of principal cells had a voltage (v(bl)) of -85.1 mv in 49 principal cells under control conditions. measures of the input resistance r(pc) together with membrane fractional resistance yielded estimates of the conductance of the basolateral membrane (g(bl) = 1.48 &mgr;s) and the apical ... | 2002 | 12770112 |
| lipid metabolism during sequential gonotrophic cycles in large and small female aedes aegypti. | the lipid metabolism was investigated during six gonotrophic cycles of aedes aegypti. females of constant body size were analyzed for their total lipid content: large females with a body size of 41.06 (wing length cubed) and small females with 15.63. their lipid contents at eclosion were compared to lipid values after two days of sugar-feeding, shortly before a blood meal, after oviposition, of their total egg batches, and again before the next blood meal, with intermittent access to sugar for t ... | 2002 | 12770082 |
| a ribosome-free extract from cultured cells improves recovery of polysomes from the mosquito fat body: analysis of vitellogenin and ribosomal protein rpl34 gene expression. | we have examined the association of ribosomal protein rpl34 mrna with polysomes in aedes albopictus c7-10 cells in culture using a simple, two-step sucrose gradient. in growing cells, 40-50% of the ribosomes were engaged on polysomes. this proportion could be increased to 80% when metabolism was stimulated by refeeding the cells with fresh medium. conversely, ribosomes shifted off polysomes when cells were starved with phosphate-buffered saline or cell lysates were treated with puromycin. when s ... | 2002 | 12770046 |
| the role of hemolymph proline as a nitrogen sink during blood meal digestion by the mosquito aedes aegypti. | mosquitoes utilize the amino acids derived from blood meal protein to produce egg proteins. but the amino acids can also be used to produce egg lipid or can be oxidized for energy production. these latter two processes result in the release of nitrogen as toxic ammonia. therefore, amino acids must be processed in such a way that amino acid nitrogen can be incorporated into non-toxic waste products. proline is the predominant amino acid in the hemolymph of the adult female mosquito aedes aegypti. ... | 2003 | 12770004 |
| sequential deposition of yolk components during oogenesis in an insect, aedes aegypti (diptera: culicidae). | vitellogenesis in aedes aegypti of uniform body size was followed at 27 degrees c in narrow time intervals throughout their first reproductive cycle by measuring the length, diameter, and volume of follicles and oocytes, the latter as an expression of the yolk mass (vitellus). independent of all experimental conditions, a two-step process of elongation was recognized for both follicle length and yolk length, so that growth curves were consistently composed of two linear regressions with differen ... | 2003 | 12770000 |
| synthesis and sar of cis-1-benzoyl-1,2,3,4-tetrahydroquinoline ligands for control of gene expression in ecdysone responsive systems. | a library of 35 cis-1-benzoyl-2-methyl-4-(phenylamino)-1,2,3,4-tetrahydroquinolines was prepared. the compounds bore various substitutuents on the benzoyl ring, at the 4-position of the phenylamino ring and at the 6-position of the tetrahydroquinoline ring. the compounds were assayed for their ability to cause expression of a reporter gene downstream of an ecdysone response element in a mammalian cell line engineered to express the ecdysone receptor from aedes aegypti. in general, compounds with ... | 2003 | 12749904 |
| stimulation of jh biosynthesis by the corpora allata of adult female aedes aegypti in vitro: effect of farnesoic acid and aedes allatotropin. | previous studies have demonstrated that the synthesis of juvenile hormone (jh) by the isolated corpora allata (ca) complex in vitro as well as the jh titer in the yellow fever mosquito aedes aegypti are elevated before feeding and low after a blood meal. in the present study, we used an in vitro radiochemical assay to analyze the effect of farnesoic acid (fa) and aedes allatotropin (aedes-at) on the biosynthesis of jh and methyl farnesoate (mf) by the isolated ca complex of a. aegypti adult fema ... | 2003 | 12728004 |
| [toxicity of the ethanol extract of magonia pubescens on larvae aedes aegypti]. | crude ethanol extracted from magonia pubescens trunks was utilized to prove its effect in the intestinal tract of aedes aegypti 3rd instar larvae. exposure times (2, 4, 6, 8, 10, 12, and 13 hours) were tested to verify when the morphological alterations begin to occur, in the intestinal tract of the larvae. the toxic effect of the extract was mainly in the midgut, beginning at the anterior midgut and followed through to posterior midgut. the main alterations observed were partial or total cell d ... | 2013 | 12715059 |
| larvicidal and chemosterilant activity of the acetone fraction of petroleum ether extract from argemone mexicana l seed. | the acetone fraction of the petroleum ether extract of seeds from argemone mexicana l. exhibited larvicidal and growth inhibiting activity against the second instar larvae of aedes aegypti (linn). this activity occurred at higher concentrations (200, 100, 50 and 25 ppm). chemosterilant activity, including reduction in blood meal utilization (27.70%), reduction in fecundity (19.00%), formation of larval-pupal intermediates, formation of pupal-adult intermediates, adult mortality and sterility of ... | 2003 | 12699943 |
| pyrethroid and ddt cross-resistance in aedes aegypti is correlated with novel mutations in the voltage-gated sodium channel gene. | samples of the dengue vector mosquito aedes aegypti (l.) (diptera: culicidae) were collected from 13 localities between 1995 and 1998. two laboratory strains, bora (french polynesia) and aeae, were both susceptible to ddt and permethrin; all other strains, except larentuka (indonesia) and bouaké (ivory coast), contained individual fourth-instar larvae resistant to permethrin. ten strains were subjected to a range of biochemical assays. many strains had elevated carboxylesterase activity compared ... | 2003 | 12680930 |
| antigenic relationships between adult and larval anopheles tessellatus midgut glycoproteins and the midguts of other vector mosquitoes. | glycoproteins expressed on the surface of midgut (mg) epithelium and the peritrophic matrix (pm) of vector mosquitoes (diptera: culicidae) are candidate molecules for interacting with pathogens. antisera produced against anopheles tessellatus theobald female mg lectin-binding proteins (concanavalin a and wheat germ agglutinin) were used in western blots to investigate mg/pm antigenic relationships between adult and larval an. tessellatus and with the mg glycoproteins of other vector mosquitoes: ... | 2003 | 12680921 |
| bioactivity of selected plant essential oils against the yellow fever mosquito aedes aegypti larvae. | the bioactivity of 14 essential oils from five plants has been studied using the brine shrimp lethality test and the aedes aegypti larvicidal assay. all essential oils screened had lc50 values smaller than 200 microg/ml, showing significant lethality against brine shrimp. in addition, nine of the 14 essential oils tested showed toxicity against the fourth-instar a. aegypti larvae in 24 h (lc50<100 microg/ml). of these, the leaf and bark essential oils of cryptomeria japonica demonstrated high la ... | 2003 | 12676507 |
| fipronil metabolism and dissipation in a simplified aquatic ecosystem. | several phenylpyrazole derivatives are selective inhibitors of chloride channel activities in insects. in this chemical family, fipronil is a powerful insecticide now widely used for several purposes. the dissipation of this molecule in a simplified aquatic ecosystem has been studied for 3 months, using (14)c-labeled fipronil. the main features of the complex process leading to fipronil transformation in this system were the following. the fipronil aqueous solution was submitted to two chemical ... | 2003 | 12590480 |
| field evaluation of cypermethrin and cyfluthrin against dengue vectors in a housing estate in malaysia. | cynoff 25ulv (cypermethrin 25 g/l) and solfac ul015 (cyfluthrin 1.5% w/v) were evaluated against the sentinel sugar-fed adults and 4th-instar larvae of aedes aegypti in a housing estate endemic of dengue in malaysia. the impact of both pyrethroids on field populations of aedes albopictus and aedes aegypti larvae was monitored weekly using bottle containers. both cynoff 25ulv and solfac ul015 showed adulticidal effects and larvicidal effects. this field trial using cynoff 25ulv against dengue vec ... | 2002 | 12546459 |
| mosquito (aedes aegypti ) aquaporin, present in tracheolar cells, transports water, not glycerol, and forms orthogonal arrays in xenopus oocyte membranes. | previous results showed that mrna encoding a putative aquaporin (aqp) (genbank accession number af218314) is present in the tracheolar cells associated with female aedes aegypti malpighian tubules. in this study, immunohistochemistry detected the protein, aeaaqp, also in tracheolar cells, suggesting its involvement in water movement in the respiratory system. when expressed in xenopus oocytes, aeaaqp increased the osmotic water permeability from 15 x 10(-6) to 150 x 10(-6) m x s-1, which was inh ... | 2003 | 12542692 |
| characterization of three toll-like genes from mosquito aedes aegypti. | three toll-related genes (aetoll1a, aetoll1b and aetoll5) were cloned and characterized from the yellow fever vector mosquito, aedes aegypti. all three genes exhibited high levels of amino acid sequence similarity with drosophila melanogaster (dm)toll1 and dmtehao (toll5). aetoll1a and aetoll1b are 1124 and 1076 amino acid residues long, respectively. both contain a carboxyl extension downstream of the toll/interleukin-1 receptor (tir) domain. aetoll5 is 1007 residues long and, like dmtehao, lac ... | 2003 | 12542637 |
| from est sequence to spider silk spinning: identification and molecular characterisation of nephila senegalensis major ampullate gland peroxidase nspox. | spider dragline silk is renowned as one of the toughest materials of its kind. in nature, spider silks are spun out of aqueous solutions under environmental conditions. this is in contrast to production of most synthetic fibres, where hazardous solvents, high temperatures and pressure are used. in order to identify some of the chemical processes involved in spider silk spinning, we have produced a collection of cdna sequences from specific regions of nephila senegalensis major ampullate gland. w ... | 2003 | 12535681 |
| [susceptibility to the infection of anopheles nuneztovari gabaldón and aedes aegypti l with romanomermis iyengari welch (rhabditida: mermitidae)]. | a laboratory-based study was performed in order to assess the infestation capacity of the mosquito parasite nematode romanomermis iyengari welch, on anopheles nuneztovari gabaldón and aedes aegypti l. for each mosquito species, nine hundred (900) i to iii instars larvae were taken. these larvae were infested with pre-parasitic larvae of r. iyengari in proportions of 5:1 and 10:1. the infestation averages obtained were 3.9 and 6.7 for anopheles nuneztovari, and 1.9 and 4.7 for aedes aegypti respe ... | 2002 | 12520998 |
| the dependence of electrical transport pathways in malpighian tubules on atp. | the relationship between the intracellular atp concentration [atp](i) and the electrical properties of principal cells was investigated in malpighian tubules of the yellow fever mosquito, aedes aegypti. under control conditions, [atp](i) was 0.91 mmol l(-1), the input resistance of the principal cell (r(pc)) was 334.1 k omega, and the basolateral membrane was marked by a large k(+)-conductance and a membrane voltage (v(bl)) of -75.8 mv. peritubular cyanide (cn, 0.3 mmol l(-1)) reduced [atp](i) t ... | 2003 | 12477894 |
| characterization and cdna cloning of an immune-induced lysozyme from cultured aedes albopictus mosquito cells. | protein chemistry and cdna sequencing were used to identify an aedes albopictus mosquito lysozyme secreted after treatment of cultured cells with heat-killed bacteria. on acid gels, the putative lysozyme activity ran just ahead of the cecropin band. elution of this activity yielded a single band on sds gels, with a mass of approximately 14 kda. mass spectral analysis of the silver-stained band uncovered five tryptic peptides with masses that matched peptides from an aedes aegypti lysozyme, which ... | 2003 | 12477497 |
| use of subtracted libraries and macroarray to isolate developmentally specific genes from the mosquito, aedes aegypti. | subtracted cdna libraries were screened with cdna macroarrays to isolate larval and pupal stage-specific genes from aedes aegypti. of 103 partial cdnas sequenced from the 4th instar subtracted cdna library, 62 have counterpart genes in other organisms while 41 of them have no significant similarity to any known genes. sequences of 116 partial cdna clones from the pupal subtracted library revealed that 57 belong to unknown genes and 59 have homologous genes in other organisms. results of cdna mac ... | 2002 | 12429127 |
| presence of chitinase and beta-n-acetylglucosaminidase in the aedes aegypti. a chitinolytic system involving peritrophic matrix formation and degradation. | measurement of the hydrolysis of specific fluorogenic substrates by spectrophotometry as well as the substrate activity-sds-page gel analysis of the chitinolytic activity in aedes aegypti guts showed that both chitinase and beta-n-acetylglucosaminidase are present and physiologically active. both enzymes were present even in guts from unfed insects, but the activities increased rapidly after feeding on blood or an artificial protein-free diet. chitinase activity was predominantly of the 'endo'-t ... | 2002 | 12429124 |
| repellent activity of constituents identified in foeniculum vulgare fruit against aedes aegypti (diptera: culicidae). | the repellent activity of materials derived from the methanol extract of fruits from foeniculum vulgareagainst hungry aedes aegypti females was examined using skin and patch tests and compared with that of the commercial n,n-diethyl-m-toluamide (deet) and (z)-9-octadecenoic acid. the biologically active constituents of the foeniculum fruits were characterized as (+)-fenchone and (e)-9-octadecenoic acid by spectroscopic analyses. responses varied according to compound, dose, and exposure time. in ... | 2002 | 12428949 |
| cloning and tissue distribution of two na+/h+ exchangers from the malpighian tubules of aedes aegypti. | a na(+)/h(+) exchanger (nhe) on the apical membrane of mosquito malpighian tubule (mt) cells is believed to participate in the blood-feeding mosquito's vital secretion of na(+) and fluid. this study presents the molecular cloning, primary structure, and tissue distribution of two cdnas encoding aedes aegypti mosquito mt nhes. the cdna sequences were obtained from mosquito mt total rna using reverse transcription-polymerase chain reaction (rt-pcr) and 5' and 3' rapid amplification of cdna ends (r ... | 2002 | 12386840 |
| operational note indoor low-volume spray trials: handheld equipment evaluation. | four handheld aerosol-mist generators and 1 thermal fog generator were evaluated initially for their ability to deliver low flow rates (< or = 5 ml/min) of resmethrin insecticide. two generators, the london fog eliminator and clarke pl, were then selected from that group to conduct a treatment of a block of 16 residences (4 x 4 grid) vs. direct treatment of individual residences to determine the most efficacious method of treating a group of small dwellings or a village. data are presented on dr ... | 2002 | 12322948 |
| effectiveness and residual activity comparison of granular formulations of insect growth regulators pyriproxyfen and s-methoprene against florida mosquitoes in laboratory and outdoor conditions. | effectiveness and residual activity tests of granular formulations of 2 insect growth regulators (igrs), s-methoprene and pyriproxyfen, against laboratory-reared larvae of 5 colonized mosquitoes, aedes aegypti, aedes albopictus, aedes taeniorhynchus, anopheles quadrimaculatus, and culex nigripalpus, were conducted in the laboratory and outdoors in plastic tubs. culex quinquefasciatus was exposed to these two igrs in the laboratory only. each igr formulation was applied at 0.02 and 0.05 ppm activ ... | 2002 | 12322941 |
| mosquito vector control and biology in latin america--a twelfth symposium. denver, colorado, february 2002. summaries. | the 12th annual latin american symposium presented by the american mosquito control association (amca) was held as part of the 68th annual meeting in denver, co, in february 2002. the principal objective, as for the previous 11 symposia, was to promote participation in the amca by vector control specialists, public health workers, and academicians from latin america. this publication includes summaries of 35 presentations that were given orally in spanish or presented as posters by participants ... | 2002 | 12322934 |
| cloning and sequence analysis of mosquito defensin genes. | to clone the defensin genes from the chinese main mosquito vectors and conduct sequnce analysis. | 2002 | 12297467 |
| molecular biology of mosquito vitellogenesis: from basic studies to genetic engineering of antipathogen immunity. | elucidation of molecular mechanisms underlying stage- and tissue-specific expression of genes activated by a blood meal is of great importance for current efforts directed towards utilizing molecular genetics to develop novel strategies of mosquito and pathogen control. regulatory regions of such genes can be used to express anti-pathogen effector molecules in engineered vectors in a precise temporal and spatial manner, designed to maximally affect a pathogen. the fat body is a particularly impo ... | 2002 | 12225918 |
| toward a description of the sialome of the adult female mosquito aedes aegypti. | to describe the set of mrna and protein expressed in the salivary glands (sialome) of aedes aegypti mosquitoes, we randomly sequenced a full-length cdna library of this insect and performed edman degradation of pvdf-transferred protein bands from salivary homogenates. we found 238 cdna clusters which contained those coding for 10 of the 11 proteins found by aminoterminal degradation. all six previously described salivary proteins were found in this library. full-length sequences of 32 novel cdna ... | 2002 | 12213246 |
| ribonucleotide reductase subunits from the yellow fever mosquito, aedes aegypti: cloning and expression. | ribonucleotide reductase catalyses the de novo synthesis of deoxyribonucleotides. class i reductases use an iron center to generate a tyrosyl free radical that can initiate formation of the deoxyribonucleotide. these reductases are alpha 2 beta 2 holoenzymes, and the subunits are denoted as r1 and r2. r1 contains the allosteric binding site and the active site, whereas r2 contains a binuclear iron center that initiates formation of the tyrosyl radical. we have cloned and sequenced the cdnas enco ... | 2002 | 12213240 |
| the chemical characterization of the epicuticular hydrocarbons of aedes aegypti (diptera: culicidae). | the chemical characterization of the hydrocarbon fraction of the epicuticular lipids of the vector mosquito aedes aegypti (linnaeus) was performed using gas chromatography (gc) and gas chromatography-electron impact mass spectrometry (gc-ms). seventy eight compounds were detected in purified hexane extracts and of these, 42 hydrocarbons were identified and several of the remaining compounds were partially characterized. the hydrocarbon classes present were n-alkanes, monomethylalkanes, dimethyla ... | 2002 | 12191436 |
| neuropeptide f and its expression in the yellow fever mosquito, aedes aegypti. | a neuropeptide f (npf) was isolated from an extract of adult aedes aegypti mosquitoes based on its immunoreactivity in a radioimmunoassay for drosophila npf. after sequencing the peptide, cdnas encoding the npf were identified from head and midgut. these cdnas encode a prepropeptide containing a 36 amino acid peptide with an amidated carboxyl terminus, and its sequence shows it to be a member of the neuropeptide f/y superfamily. immunocytochemistry and northern blots confirmed that both the brai ... | 2002 | 12182937 |
| leucokinin activates ca(2+)-dependent signal pathway in principal cells of aedes aegypti malpighian tubules. | the role of ca(2+) in mediating the diuretic effects of leucokinin-viii was studied in isolated perfused malpighian tubules of the yellow fever mosquito, aedes aegypti. peritubular leucokinin-viii (1 microm) decreased the transepithelial resistance from 11.2 to 2.6 komega. cm, lowered the transepithelial voltage from 42.8 to 2.7 mv, and increased transepithelial cl(-) diffusion potentials 5.1-fold. in principal cells of the tubules, leucokinin-viii decreased the fractional resistance of the baso ... | 2002 | 12167601 |
| linkage map organization of expressed sequence tags and sequence tagged sites in the mosquito, aedes aegypti. | a composite genetic linkage map for the yellow fever mosquito aedes aegypti was constructed based on restriction fragment length polymorphism (rflp), single nucleotide polymorphism (snp) and single strand conformation polymorphism (sscp) markers. the map consists of 146 marker loci distributed across 205 cm, and includes several morphological mutant marker loci. most of the genetic markers are derived from random cdnas or ae. aegypti genes of known function. a number of markers are derived from ... | 2002 | 12144703 |
| does temperature affect the outcome of larval competition between aedes aegypti and aedes albopictus? | the superior larval competitive ability of aedes albopictus has been proposed to explain the recent displacement of aedes aegyptiby the former species inparts of the southeastern u.s. ae. aegypti persists, however, in sympatry with ae. albopictus in urban areas of southern louisiana, florida, and texas, and the impact of larval competition between these species has not been investigated at higher temperatures that may be characteristic of these urban environments. we compared growth and survivor ... | 2002 | 12125878 |
| evaluation of various models of propane-powered mosquito traps. | large cage and field studies were conducted to determine the efficacy of various models of propane-powered mosquito traps. these traps utilized counterflow technology in conjunction with catalytic combustion to produce attractants (carbon dioxide, water vapor, and heat) and a thermoelectric generator that converted excess heat into electricity for stand-alone operation. the cage studies showed that large numbers of aedes aegypti and ochlerotatus taeniorhynchus were captured and that each progres ... | 2002 | 12125861 |
| an ecological survey of dengue vector mosquitos in central lao pdr. | an ecological survey of dengue vector mosquitos was carried out in june 2000 in central lao pdr. two areas in khammouane province, nongbok and thakhek, were selected for the survey. of the 7 mosquito species identified, aedes aegypti was dominant in both study areas. the container index for ae. aegypti in nongbok was 51.8% and was significantly higher than that of thakhek (40.2%); moreover, significant differences between the study areas were found with records to containers and to the condition ... | 2002 | 12118462 |
| a genetic correlation between age at pupation and melanization immune response of the yellow fever mosquito aedes aegypti. | to investigate the evolutionary cost of an immune response, we selected six lines of the mosquito aedes aegypti for earlier or later pupation and measured the extent to which this selection procedure changed the mosquito's ability to encapsulate and melanize a negatively charged sephadex bead. after 10 generations of selection, the age at pupation in the two selection regimes differed by about 0.7 days, accompanied by an increase of wing length of the mosquitoes selected for late pupation. among ... | 2002 | 12093022 |
| mouthparts of male aedes (stegomyia) mosquitoes. | mouthparts of adult males of 17 strains of 8 species from the subgenus stegomyia of the genus aedes, including 5 strains of aedes aegypti and 6 strains of aedes albopictus, were examined. lengths of maxillae, mandibles, maxillary palpi, and proboscises were measured under light microscopy and their detailed structures were examined by scanning electron microscopy. lengths were presented as ratios to proboscis lengths. in contrast to previous reports, mandibles were found in all 5 strains of male ... | 2002 | 12083358 |
| point source inoculation of mesocyclops (copepoda: cyclopidae) gives widespread control of ochlerotatus and aedes (diptera: culicidae) immatures in service manholes and pits in north queensland, australia. | this study details the novel application of predacious copepods, genus mesocyclops, for control of ochlerotatus tremulus (theobald) group and aedes aegypti (l.) mosquito larvae in subterranean habitats in north queensland, australia. during june 1997, 50 mesocyclops sp. 1 were inoculated into one service manhole in south townsville. wet season rainfall and flooding in both 1998 and 2000 was responsible for the dispersal of copepods via the underground pipe system to 29 of 35 manholes over an are ... | 2002 | 12061442 |
| a piperidine amide extracted from piper longum l. fruit shows activity against aedes aegypti mosquito larvae. | mosquito larvicidal activity of piper longum fruit-derived materials against the fourth-instar larvae of aedes aegypti was examined. a crude methanol extract of p. longum fruits was found to be active against the larvae, and the hexane fraction of the methanol extract showed a strong larvicidal activity of 100% mortality. the biologically active component of p. longum fruits was characterized as pipernonaline by spectroscopic analyses. the lc(50) value of pipernonaline was 0.25 mg/l. the toxicit ... | 2002 | 12059157 |
| neuroendocrine modulation of olfactory sensory neuron signal reception via axo-dendritic synapses in the antennae of the mosquito, aedes aegypti. | an ultrastructural study of the antennae of the yellow fever mosquito, aedes aegypti, revealed that as in the salt marsh mosquito, culex salinarius, the first flagellar segment of both sexes of a. aegypti contain neuroendocrine cells. these cells not only extend an axon via the antennal nerve to the antennal lobe of the deutocerebrum, but project collaterals to the periphery of the antennae, where they modulate the antennal sensory neurons by forming synapses with the dendrites of these afferent ... | 2002 | 12059042 |
| derris (lonchocarpus) urucu (leguminosae) extract modifies the peritrophic matrix structure of aedes aegypti (diptera:culicidae). | aqueous suspension of ethanol extracts of derris (lonchocarpus) urucu (leguminosae), collected in the state of amazonas, brazil, were tested for larvicidal activity against the mosquito aedes aegypti (diptera:culicidae). the aim of this study was to observe the alterations of peritrophic matrix in ae. aegypti larvae treated with an aqueous suspension of d. urucu extract. different concentrations of d. urucu root extract were tested against fourth instar larvae. one hundred percent mortality was ... | 2002 | 12051197 |
| variation of the oviposition preferences of aedes aegypti in function of substratum and humidity. | two aedes aegypti (l.) populations were studied in the laboratory regarding the preference for three types of breeding sites, i.e., flasks containing only water, flasks with a plant and flasks with a stick. each of these breeding units was placed in one cage and the choice of the oviposition sites was determined for individual females and three females per experimental unit at two humidity levels. preference for ovipositing on the water surface was observed and varied according to experimental u ... | 2002 | 12048575 |
| formulation of an effective mosquito-repellent topical product from lemongrass oil. | ointment and cream formulations of lemongrass oil in different classes of base and the oil in liquid paraffin solution have been evaluated for mosquito repellency in a topical application. mosquito repellency was tested by determining the bite-deterrence of product samples applied on an experimental bird's skin against a 2-day starved culture of aedes aegypti l. mosquitoes. the 1%v/v solution and 15%v/w cream and ointment preparations of the oil exhibited > or =50% repellency lasting 2-3 h, whic ... | 2002 | 12046869 |
| software for pattern recognition of the larvae of aedes aegypti and aedes albopictus. | software for pattern recognition of the larvae of mosquitoes aedes aegypti and aedes albopictus, biological vectors of dengue and yellow fever, has been developed. rapid field identification of larva using a digital camera linked to a laptop computer equipped with this software may greatly help prevention campaigns. | 2003 | 12045822 |
| [climate and the superimposed distribution of aedes aegypti and aedes albopictus on infestation of são paulo state, brazil]. | to study the influence of climatic factors and superimposed distribution of aedes aegypti and aedes albopictus populations on their geographic dispersal in the state of são paulo. | 2002 | 12045797 |
| characterization of hemocytes from the yellow fever mosquito, aedes aegypti. | mosquitoes are the most important arthropod disease vectors, transmitting a broad range of pathogens that cause diseases such as malaria, lymphatic filariasis, and yellow fever. mosquitoes and other insects are able to mount powerful cellular and humoral immune responses against invading pathogens. to date, most studies have concentrated on the humoral response. in the current study we describe the hemocytes (blood cells) of the yellow fever mosquito, aedes aegypti, by means of morphology, lecti ... | 2002 | 12029490 |
| comparison of cetirizine, ebastine and loratadine in the treatment of immediate mosquito-bite allergy. | people frequently experience whealing and delayed papules from mosquito bites. whealing is mediated by antisaliva immunoglobulin (ig)e antibodies and histamine. cetirizine, ebastine and loratadine have earlier shown effects on mosquito-bite reactions but no comparative studies exist. | 2002 | 12028119 |
| highly-substrate active isoenzyme acetylcholinesterase-ii, in rosy eye mutant of aedes aegypti mosquito. | insecticide bioassays were carried out on larvae and adults of rosy eye mutant and wildtype strains of a. aegypti. both the strains were equally susceptible to ddt, malathion and deltamethrin. biochemical assays showed an increase in acetylcholinesterase enzyme (ache) activity in all the stages of mutant strain with both the substrates i.e. acetylthiocholine iodide and s-butyrylthiocholine iodide. however, there was no difference in the percent inhibition of enzyme activity with propoxur in thes ... | 2001 | 12018585 |
| glucosamine:fructose-6-phosphate aminotransferase: gene characterization, chitin biosynthesis and peritrophic matrix formation in aedes aegypti. | glucosamine:fructose-6-phosphate aminotransferase (gfat) catalyses the formation of glucosamine 6-phosphate and is the first and rate-limiting enzyme of the hexosamine biosynthetic pathway. the final product of the hexosamine pathway, udp-n-acetyl glucosamine, is an active precursor of numerous macromolecules containing amino sugars, including chitin in fungi and arthropods. chitin is one of the essential components of insect cuticle and peritrophic matrix. the peritrophic matrix is produced in ... | 2002 | 12000639 |
| survey of aedes aegypti eggs in and around homes in tucson, arizona. | the abundance and location of eggs of aedes aegypti were evaluated through inspection of 24 tucson homes during the winter of 1998-99 to assess the potential of the mosquitoes to overwinter in this area. eggs were found either indoors or outdoors in 46% of residences surveyed. after immersion in water, about 23% of eggs found indoors hatched, and about 5% of eggs found outdoors hatched. | 2002 | 11998933 |
| two isoforms of the early e74 gene, an ets transcription factor homologue, are implicated in the ecdysteroid hierarchy governing vitellogenesis of the mosquito, aedes aegypti. | in the anautogenous mosquito, aedes aegypti, vitellogenesis is under the strict control of 20-hydroxyecdysone (20e), which is produced via a blood meal-activated hormonal cascade. several genes of the ecdysteroid-regulatory hierarchy are conserved between vitellogenesis in mosquitoes and metamorphosis in drosophila. we report characterization of two isoforms of the mosquito early e74 gene (aae74), which have a common c-terminal ets dna-binding domain and unique n-termini. they exhibited a high l ... | 2002 | 11997188 |
| development and applications of transgenesis in the yellow fever mosquito, aedes aegypti. | transgenesis technology has been developed for the yellow fever mosquito, aedes aegypti. successful integration of exogenous dna into the germline of this mosquito has been achieved with the class ii transposable elements, hermes, mariner and piggybac. a number of marker genes, including the cinnabar(+) gene of drosophila melanogaster, and fluorescent protein genes, can be used to monitor the insertion of these elements. the availability of multiple elements and marker genes provides a powerful ... | 2002 | 11985858 |
| effect of the synergist, piperonyl butoxide, on the development of deltamethrin resistance in yellow fever mosquito, aedes aegypti l. (diptera: culicidae). | the larvae and adults of aedes aegypti were tested for the potential to develop resistance to the synthetic pyrethroid, deltamethrin, alone or a combination of deltamethrin with the synergist, piperonyl butoxide (pbo). although continuous larval selection for 40 generations resulted in 703-fold resistance, the resistance ratio in the adults was only 1.3. similarly, adult selections with deltamethrin showed a resistance ratio of less than four after 40 generations, indicating differential respons ... | 2002 | 11948970 |
| investigation of the larvicidal activity of pothomorphe peltata and isolation of the active constituent. | the insecticidal activity of the leaves of pothomorphe peltata (piperaceae) was evaluated on aedes aegypti larvae. the active methanol extract was subjected to a bioactivity-directed fractionation, monitored by the larvicidal assay, and led to the isolation of a catechol derivative, 4-nerolidylcatechol. this compound was quite potent against aedes aegypti larvae (lc(50) = 9.1 microg/ml). | 2002 | 11933144 |
| winter intervention against aedes aegypti (diptera: culicidae) larvae in subterranean habitats slows surface recolonization in summer. | at semiarid charters towers, north queensland, australia, the importance of aedes aegypti (l.) in wells was assessed in relation to the colonization of surface habitats during the wet season. from april to july 1999, 10 wells (five positive for ae. aegypti) were monitored to assess their status and larvae population numbers therein. all surface containers located within a 100 m radius of each well were removed, treated with s-methoprene or sealed to prevent the utilization of these containers by ... | 2002 | 11931036 |
| characterization of the aeahp gene and its expression in the mosquito aedes aegypti (diptera: culicidae). | the sequence and tissue expression of the gene encoding a peptide hormone aea-hp-i, known to inhibit host-seeking behavior, has been characterized for the yellowfever mosquito, aedes aegypti (l.). the open reading frame reveals a prepropeptide that would be processed into three identical peptides. the gene contains four short introns and exists as a single genomic copy. transcripts of the gene were present in the brain, terminal ganglion, and midgut of adults, and in females, its expression prof ... | 2002 | 11931033 |
| carbonic anhydrase in the midgut of larval aedes aegypti: cloning, localization and inhibition. | the larval mosquito midgut exhibits one of the highest ph values known in a biological system. while the ph inside the posterior midgut and gastric caeca ranges between 7.0 and 8.0, the ph inside the anterior midgut is close to 11.0. alkalization is likely to involve bicarbonate/carbonate ions. these ions are produced in vivo by the enzymatic action of carbonic anhydrase. the purpose of this study was to investigate the role of this enzyme in the alkalization mechanism, to establish its presence ... | 2002 | 11907049 |
| aedes aegypti peritrophic matrix and its interaction with heme during blood digestion. | a large amount of heme is produced upon digestion of red cell hemoglobin in the midgut of mosquitoes. the interaction between heme and the peritrophic matrix (pm) was studied in aedes aegypti. by light microscopy, the pm appeared as a light brownish layer between the intestinal epithelium and the alimentary bolus. this natural color can be attributed to the presence of heme bound to the matrix. in histochemical studies, a diffuse peroxidase activity of the heme molecules was clearly observed bet ... | 2002 | 11891128 |
| organization and developmental expression of the mosquito vitellogenin receptor gene. | vitellogenin is a precursor of the major yolk protein, vitellin. it is internalized by developing oocytes via receptor-mediated endocytosis. previously, we characterized the vitellogenin receptor (vgr) from oocytes of the mosquito aedes aegypti [sappington, t.w., kokoza,v.a., cho,w.l. and raikhel,a.s. (1996) molecular characterization of the mosquito vitellogenin receptor reveals unexpected high homology to the drosophila yolk protein receptor. proc natl acad sci usa 93: 8934-8939]. the vgr rece ... | 2001 | 11881811 |
| msqtc3, a tc3-like transposon in the yellow fever mosquito aedes aegypti. | a novel transposon, msqtc3-aa, has been discovered in the yellow fever mosquito, aedes aegypti. evidence of its past mobility is presented. there are approximately 100 copies of msqtc3-aa in a. aegypti, eight of which have been isolated and sequenced. all sequenced copies are more than 99% identical to their consensus, indicating recent mobilization. the msqtc3-aa consensus contains imperfect terminal inverted repeats (tirs) and an open reading frame (orf) interrupted by an intron. sequence, str ... | 2001 | 11881806 |
| 3-hydroxykynurenine transaminase identity with alanine glyoxylate transaminase. a probable detoxification protein in aedes aegypti. | this study describes the functional characterization of a specific mosquito transaminase responsible for catalyzing the transamination of 3-hydroxykynurenine (3-hk) to xanthurenic acid (xa). the enzyme was purified from aedes aegypti larvae by ammonium sulfate fractionation, heat treatment, and various chromatographic techniques, plus non-denaturing electrophoresis. the purified transaminase has a relative molecular mass of 42,500 by sds-page. n-terminal and internal sequencing of the purified p ... | 2002 | 11880382 |
| dengue in children: critical points in management. | dengue fever (df) is endemic and occasionally epidemic in many tropical and subtropical areas inhabited by the mosquito vector aedes aegypti. infection with dengue may present with varied clinical manifestations. significant morbidity and mortality can result if early recognition and monitoring of its severe forms (dhf and dss) is not done and if appropriate medical intervention is delayed. this article reviews several critical points in the basic evaluation and management of dengue in the pedia ... | 2001 | 11845665 |
| [high esterases as mechanism of resistance to organophosphate insecticides in aedes aegypti strains]. | five strains of aedes aegypti l. one from cuba and 4 from venezuela were evaluated to determine their resistance to organophosphate insecticides (temephos, chlorpiriphos and pirimiphos methyl). in the venezuelan strains only apure showed resistance to temephos. in tachira and miranda moderate resistance values were noted (fr50 5 to 10x) for chlorpiriphos and high levels of resistance (fr > 10x) to this insecticide were found in aragua. all the venezuelan strains showed high levels of resistance ... | 2009 | 11826536 |
| [adaptation of microtitration plate methods for quantification of the activity of esterases and glutathione-s-transferase in aedes aegypti]. | the detection techniques for the activity of non-specific esterases and glutathione-s-transferase in culex quinquefasciatus were modified to detect such enzymes in aedes aegypti(l). the optimal concentration values of substrate (saturating concentration) and the optimum reading time for reaction were determined for each enzyme by using 4 aedes aegypti strains: one from cuba 2 from venezuela and one susceptible reference strain. the frequency of non-specific esterases turned out to be 0.76 in mir ... | 2009 | 11826535 |
| survey of container-breeding mosquitoes from the florida keys, monroe county, florida. | a survey of container-breeding mosquitoes was conducted on urban islands (big coppitt key, rockland key, key west, and stock island) and rural islands (big pine key, cudjoe key, little torch key, no name key, ramrod key, saddlebunch keys, sugarloaf key, and summerland key) within the florida keys. five mosquito species were collected: aedes aegypti, culex nigripalpus, cx. quinquefasciatus, cx. salinarius, and ochlerotatus taeniorhynchus. plastic buckets, trash cans, and discarded plastic contain ... | 2001 | 11804461 |
| oviposition attractancy of an infusion from a wood inhabiting fungus for vector mosquitoes. | review of literature indicates that none of the chemicals so far studied for oviposition attractancy was found to be promising for operational use. efforts are being made to find an effective substance to corner a large number of ovipositing females to selective breeding sites for ease of control operation. this will reduce insecticidal consumption and will be cost-effective. this study is aimed to evaluate the oviposition attractancy of aqueous infusion from a wood inhabiting fungus (polyporus ... | 2001 | 11762202 |
| stereochemical effects in an insect repellent. | racemic 1-[3-cyclohexen-1-ylcarbonyl]-2-methylpiperidine repels blood-feeding arthropods such as mosquitoes, chiggers, and ticks. the compound contains two asymmetric carbon atoms and the racemate consists of four stereoisomers. quantitative mosquito bioassays using aedes aegypti (l.) showed that (1s,2's) and (1r,2's) configurations were 2.8-3.1 and 1.6-1.8 times more effective, respectively, than the other two stereoisomers in reducing mosquito bites. (1s,2's) was 2.5 more repellent than the ra ... | 2001 | 11761378 |
| [emergence of aedes albopictus in artificial containers]. | to estimate the potential of daily average emergence of aedes albopictus adult females. since this species is very close to aedes aegypti, although it has not been implicated in the spread of dengue in brazil. | 2001 | 11723517 |
| a predictor variable for efficacy of lagenidium giganteum produced in solid-state cultivation. | lagenidium giganteum was cultivated on solid media in the absence of free water and evaluated for efficacy against second-instar aedes aegypti mosquito larvae in 100-ml bioassays. bioassay variables included level of media addition, co(2) evolution rate (cer) and cell density. logistic regression was performed on bioassay infection observations to determine if the tested variables were correlated to the probability of attaining at least 80% infection. both cer (p=0.003) and number of cells (p=0. ... | 2001 | 11687931 |
| aedes aegypti glutamine synthetase: expression and gene structure. | the peritrophic matrix (pm) is the first natural barrier a mosquito-borne parasite faces when ingested with a blood meal; consequently, understanding the biology of pm formation could provide novel transmission control strategies. because the pm is composed of chitin (a molecule of repeating units of n-acetyl glucosamine), glycoproteins and glucose, characterizing the regulation of enzymes involved in chitin production should provide information concerning factors that influence pm formation. we ... | 2001 | 11674996 |
| efficient transformation of the yellow fever mosquito aedes aegypti using the piggybac transposable element vector pbac[3xp3-egfp afm]. | we report efficient germ-line transformation in the yellow fever mosquito aedes aegypti accomplished using the piggybac transposable element vector pbac[3xp3-egfp afm]. two transgenic lines were established and characterized; each contained the vg-defensin a transgene with strong eye-specific expression of the enhanced green fluorescent protein (egfp) marker gene regulated by the artificial 3xp3 promoter. southern blot hybridization and inverse pcr analyses of genomic dna demonstrated a precise ... | 2001 | 11583926 |
| stable, germ-line transformation of culex quinquefasciatus (diptera: culicidae). | a hermes-based transposable element transformation system incorporating an enhanced green fluorescent protein (egfp) marker was used to produce two transgenic lines of culex quinquefasciatus (say). the transformation frequency was approximately 12% and transformation of culex was shown to be dependent on the presence of hermes transposase. injected culex embryos were treated with four different heat shock regimes, two of which produced transformed individuals. these individuals were mated with w ... | 2001 | 11580043 |
| deltamethrin tolerance & associated cross resistance in aedes aegypti from mysore. | the indiscriminate use of insecticides in public health and agriculture has led to the development of resistance to these insecticides in the vector mosquitoes. to understand the development of resistance to synthetic pyrethroids, selection studies on aedes aegypti were done at mysore. | 2001 | 11525152 |
| cloning and expression analysis of a 5ht7-like serotonin receptor cdna from mosquito aedes aegypti female excretory and respiratory systems. | in the mosquito aedes aegypti, 5-ht changes the endogenous rhythm of contractions in the female hindgut and increases fluid secretion in the larval malpighian tubule. the role of 5-ht as a diuretic hormone in adults has been questioned. we cloned a cdna encoding a serotonin receptor from a female a. aegypti malpighian tubule library that is similar to the 5-ht7 receptor from drosophila melanogaster. the transcript was localized in the tracheolar cells associated with the female malpighian tubule ... | 2001 | 11520359 |
| two-dimensional electrophoretic analysis of aedes aegypti mosquito fat body proteins during a gonotropic cycle. | we have used two-dimensional polyacrylamide gel electrophoresis to examine changes in the relative abundance and diversity of non-secreted proteins in aedes aegypti fat body preparations during a reproductive cycle. electrophoretic profiles were evaluated at four time points after eclosion, and at five time points after a blood meal. in contrast to the dramatic changes in abundance of specific secreted proteins such as vitellogenin, our results show that the complement of proteins internal to th ... | 2001 | 11504406 |
| the application of ethanol-extracted gloriosa superba for metaphase chromosome preparation in mosquitos. | the application of ethanol-extracted gloriosa superba for metaphase chromosome preparation in adult and 4th larva aedes aegypti revealed that 0.5-8% ethanol-extracted gl. superba solution could be used instead of 1% colchicine in hanks' balanced salt solution. for adult mosquitos, the metaphase rates and average number of metaphase chromosomes per positive mosquito after intrathoracic inoculation with 1-2% ethanol-extracted gl. superba solution were 100% and 11.8 (2-16) -12.6 (3-28) in females, ... | 2001 | 11485100 |
| susceptibility of adult field strains of aedes aegypti and aedes albopictus in singapore to pirimiphos-methyl and permethrin. | the susceptibilities of field strains (fl) of aedes aegypti and ae. albopictus adult females to pirimiphos-methyl and permethrin were investigated and compared with a susceptible laboratory strain (s) by using a filter paper (12 x 15 cm) impregnation method. the resistance ratio between the 50% lethal concentration values (rr50) of the fl and the s strains shows that the rr50 of fl ae. aegypti for pirimiphos-methyl was 1.5 and the rr50 of fl ae. aegypti for permethrin was 12.9. the rr50 of fl ae ... | 2001 | 11480824 |
| laboratory evaluation of two native fishes from tropical north queensland as biological control agents of subterranean aedes aegypti. | the ability of 2 freshwater fishes, eastern rainbow fish melanotaenia splendida splendida and fly-specked hardyhead craterocephalus stercusmuscarum stercusmuscarum, native to north queensland to prey on immature aedes aegypti was evaluated under laboratory conditions. the predation efficiency of the 2 species was compared to the exotic guppy, poecilia reticulata, which is commonly used as a biological control agent of mosquito larvae. of the 3 fish species tested, m. s. splendida was shown to be ... | 2001 | 11480819 |
| aedes aegypti: size, reserves, survival, and flight potential. | female aedes aegypti of small and large body sizes were fed ad libitum from eclosion with eight different concentrations of sucrose from 0.1% to 50%; females were also starved with access to water. for each experiment we determined the survivorship of such populations. the 90%, 50%, 10%, and maximal survivorships followed linear regressions with the logarithm of the sucrose concentration. for each condition we measured the extent of synthesis of glycogen and lipid reserves. there was a critical ... | 2001 | 11469181 |
| effect of feronia limonia on mosquito larvae. | bioassay guided fractionation of the acetone extract of feronia limonia dried leaves afforded a potent mosquito larvicide, identified as n-hexadecanoic acid and found to be effective against fourth instar larvae of culex quinquefasciatus, anopheles stephensi and aedes aegypti, with lc50 of 129.24, 79.58 and 57.23 ppm, respectively. | 2000 | 11449505 |
| cdna cloning and transcript distribution of two different neuroparsin precursors in the desert locust, schistocerca gregaria. | neuroparsins were originally identified in locust corpus cardiacum extracts as folliculostatic or 'antigonadotropic' neuropeptides. this paper presents the cloning of two different neuroparsin precursor cdnas from the brain of the desert locust, schistocerca gregaria. the first transcript encodes the precursor (scg-npp1) of s. gregaria neuroparsin a and b, whereas the second codes for a novel neuroparsin-related peptide precursor (scg-npp2). both precursors display significant sequence similarit ... | 2001 | 11422514 |
| localization of an insulin-like peptide in brains of two flies. | a nucleotide sequence (genbank ai134194) was identified in the database of the berkeley drosophila genome project based on the similarity of its translated sequence to insulin-like peptides known for other insects. the putative a chain of the drosophila peptide was synthesized and used to produce an antiserum for immunocytochemistry. medial neurosecretory cells and their axons were immunostained in whole brains of drosophila larvae and adults. in larvae, immunostained axons from these cells exte ... | 2001 | 11396725 |
| involvement of ligninlike compounds in toxicity of dietary alder leaf litter against mosquito larvae. | the toxicological characteristics of dietary decomposed alder leaf litter against mosquito larvae were further investigated through enzymatic and chemical purification of a phenoliclike cell-wall fraction isolated from crude litter. the toxicity of the subfractions obtained was controlled by standard bioassays on third instars of aedes aegypti chosen as a reference target species. enzymatic hydrolyses of the cell-wall fraction were performed with caylase, pectolyase, esterase, and beta-glycosida ... | 2001 | 11382061 |