| infection and pathogenicity of the mosquito densoviruses aednv, hednv, and apednv in aedes aegypti mosquitoes (diptera: culicidae). | these studies compared three genetically distinct mosquito densoviruses aedes aegypti (aednv), hemagogus equinus (hednv), and aedes peruvian (apednv) densoviruses in a laboratory investigation to begin to evaluate their potential as mosquito control agents. a real-time polymerase chain reaction (pcr) assay for quantification of viral genomes and a standardized mosquito infection protocol were developed. mortality associated with exposure to aednv increased in a dose-dependent manner, with the ma ... | 2004 | 15666733 |
| molecular cloning and transcriptional activation of lysozyme-encoding cdnas in the mosquito aedes aegypti. | lysozymes are enzymes characterized by their ability to break down bacterial cell walls. in insects certain lysozymes are only found in the midgut, whereas others are only found in the haemolymph and fat body after immune challenge. we identified two lysozyme-encoding cdnas from aedes aegypti. both deduced protein sequences are basic in nature, contain 148 amino acids including eight highly conserved cysteine residues, and their genomic sequences contain a single intron. transcriptional profiles ... | 2005 | 15663778 |
| aedes aegypti transferrin. gene structure, expression pattern, and regulation. | mosquitoes and all other insects so far examined have an abundant haemolymph transferrin (tsf). the exact function of these proteins has not been determined, but they may be involved in iron transport, in oogenesis and in innate immune defence against parasites and pathogens. the tsf gene of aedes aegypti has been cloned and sequenced. it contains a single small intron, which contrasts it to vertebrate tsf genes that contain up to sixteen introns. the promoter region of the gene is rich in putat ... | 2005 | 15663777 |
| larvicidal activity of plant extracts used alone and in combination with known synthetic larvicidal agents against aedes aegypti. | larvicidal activity of acetone and petroleum ether extracts of four plants murraya koenigii, coriandrum sativum, ferula asafoetida, trigonella foenum graceum and synthetic larvicides fenthion and temephos used alone and in combination was carried out against a. aegypti larvae under laboratory conditions. optimum values were found out by using different concentrations of each plant (ranged 25 ppm-900 ppm) and both synthetic larvicides (ranged 0.01-7.5 ppm). the synergistic study was carried out b ... | 2004 | 15623234 |
| characterization of a silkworm thioredoxin peroxidase that is induced by external temperature stimulus and viral infection. | a thioredoxin peroxidase (tpx) that reduces h(2)o(2) was firstly characterized in the lepidopteran insect, silkworm bombyx mori. the b. mori tpx (bmtpx) cdna contains an open reading frame of 585 bp encoding 195 amino acid residues and possesses two cysteine residues that are characteristic of 2-cys subgroup of peroxiredoxin family. the deduced amino acid sequence of the bmtpx cdna showed 78% identity to drosophila melanogaster (dmtpx-1), 73% to aedes aegypti (aatpx), and 54-48% to other insect ... | 2005 | 15607657 |
| behavioral responses of aedes aegypti (diptera: culicidae) exposed to deltamethrin and possible implications for disease control. | behavioral responses of nine aedes aegypti (l.) strains, six from recent field collections and three from the long-established laboratory colonies, were tested under laboratory-controlled conditions by using an excito-repellency test system. all nine strains showed significant behavioral escape responses when exposed to deltamethrin at the standard field dose (0.02 g/m2), regardless of background insecticide susceptibility status (susceptible or tolerant/resistant). insecticide contact irritancy ... | 2004 | 15605644 |
| reinvestigation of an endogenous meiotic drive system in the mosquito, aedes aegypti (diptera: culicidae). | we have initiated efforts to determine the molecular basis for the m(d) meiotic drive system in the mosquito, aedes aegypti. the effect of the m(d) gene is a highly male-biased sex ratio, but varies depending on the frequency and sensitivity of a susceptible responder m(s) allele. the m(d) system has potential as a mechanism for driving trangenes for pathogen resistance into natural ae. aegypti populations. because all previously existing laboratory strains carrying the m(d) gene have been lost, ... | 2004 | 15605641 |
| formation and loss of large, unstable tandem arrays of the piggybac transposable element in the yellow fever mosquito, aedes aegypti. | the class ii transposable element, piggybac, was used to transform the yellow fever mosquito, aedes aegypti. in two transformed lines only 15-30% of progeny inherited the transgene, with these individuals displaying mosaic expression of the egfp marker gene. southern analyses, gene amplification of genomic dna, and plasmid rescue experiments provided evidence that these lines contained a high copy number of piggybac transformation constructs and that much of this dna consisted of both donor and ... | 2004 | 15587266 |
| laboratory studies on the predatory potential of dragon-fly nymphs on mosquito larvae. | biocontrol potential of dragonfly nymph, brachythemis contaminata fabricius against the larvae of anopheles stephensi, culex. quinquefasciatus and aedes aegypti was studied under laboratory conditions. it was found that dragonfly nymph had highest predation efficacy against an. stephensi followed by cx. quinquefasciatus and ae. aegypti. feeding rate increased with decrease in prey size/stage. analysis of data indicated that dragonfly nymphs have good predatory potential and can be used as a biol ... | 2003 | 15562955 |
| photoelectric sensing device for recording mosquito host-seeking behavior in the laboratory. | a new automatic device for recording the host-seeking behavior of mosquitoes was designed using photoelectric sensors. the host-seeking rhythms of several species of mosquitoes were recorded under laboratory conditions. use of co2, in addition to heating and black color to activate mosquito flight, proved to be important for evaluation of the present recording device. the diel rhythms of nonblood-fed aedes albopictus (skuse), aedes aegypti (l.), culex quinquefasciatus say, culex tritaeniorhynchu ... | 2004 | 15535615 |
| comparative efficacy of ir3535 and deet as repellents against adult aedes aegypti and culex quinquefasciatus. | arm-in-cage laboratory evaluations of 2 proprietary formulations of the mosquito repellents ir3535 and n,n-diethyl-3-methylbenzamide (deet; aqueous cream, hydroalcoholic spray) were made with 10 and 20% concentrations of each repellent. also, 4 commercially available products containing ir3535 (expedition insect repellent 20.07% active ingredient [ai], bug guard plus with spf30 sunscreen 7.5% ai, bug guard plus with spf15 sunscreen 7.5% ai, and bug guard plus 7.5% ai) were tested. all comparison ... | 2004 | 15532931 |
| efficacy of methyl bromide fumigation and insecticidal dips against aedes eggs on lucky bamboo. | importation of lucky bamboo (dracaena sp.) stalk clusters has led to the accidental importation of aedes albopictus in some countries. current methods of fumigation using methyl bromide are harmful to live plants and thus are not acceptable. we exposed aedes aegypti eggs to methyl bromide at rates of 32 g/m3 for 2 and 3 h and 48 g/m3 for 3 h, achieving complete control in 0/5, 1/5, and 5/5 replicates, respectively. submersion for 1 min in aqueous solutions of 0.04% active ingredient (ai) lambda- ... | 2004 | 15532928 |
| larvicidal action of ethanolic extracts from fruit endocarps of melia azedarach and azadirachta indica against the dengue mosquito aedes aegypti. | ethanolic extracts from the kernels of ripe fruits from the indian lilac melia azedarach and from the well-known neem tree, azadirachta indica were assayed against larvae of aedes aegypti, the mosquito vector of dengue fever. the lethality bioassays were carried out according to the recommendations of the world health organization. extracts were tested at doses ranging from 0.0033 to 0.05 g% in an aqueous medium for 24 and 48 h, at 25 or 30 degrees c, with or without feeding of the larvae. lc50, ... | 2004 | 15530964 |
| molecular characterization of a novel peroxidase involved in aedes aegypti chorion protein crosslinking. | peroxidase-catalyzed chorion or eggshell protein crosslinking is an important biochemical event contributing to the formation of a protective chorion or eggshell in insects. although the survival of the developing or developed embryo in the environment before hatching relies on the protection of the chorion, the identity of the peroxidative enzyme responsible for mediating chorion protein crosslinking has never been identified in any insect species. in this report, we describe the determination ... | 2004 | 15522615 |
| chemical composition and larvicidal activity of the essential oils from eupatorium betonicaeforme (d.c.) baker (asteraceae). | the volatile composition of the essential oils from leaves and roots of eupatorium betonicaeforme (d.c.) baker was analyzed by gc-ms. a total of 12 compounds were identified. beta-caryophyllene (12.4-41.7%), alpha-humulene (11.7-14.6%), gamma-muurolene (10.4-19.0%), bicyclogermacrene (15.0-17.5%), 2,2-dimethyl-6-vinylchroman-4-one (10.3-25.5%), and 2-senecioyl-4-vinylphenol (8.5-41.0%) were the most prominent constituents. the former two compounds were isolated and characterized by spectroscopic ... | 2004 | 15506805 |
| the aeact-4 gene is expressed in the developing flight muscles of female aedes aegypti. | population reduction of mosquitoes is an effective method for controlling dengue fever and malaria transmission. recent developments in control techniques include proposals to construct transgenic strains of mosquitoes carrying dominant, conditional-lethal genes under the control of sex- and stage-specific promoters. in order to identify such promoters, subtractive cdna libraries derived from male and female pupal mrna of the yellow fever mosquito, aedes aegypti, were constructed and screened. a ... | 2004 | 15373813 |
| expression of a sterol carrier protein-x gene in the yellow fever mosquito, aedes aegypti. | the sterol carrier protein-x (scp-x), a peroxisomal thiolase/nonspecific lipid binding protein, was characterized in the yellow fever mosquito, aedes aegypti. the aedes aegypti scp-x (aescp-x) has 83% and 75% similarities to drosophila and mammalian scp-x, respectively. however, the aescp-x gene did not produce multiple transcripts, which is characteristic of the vertebrate scp-x gene. levels of aescp-x transcription were higher in larvae and pupae. gut tissue showed the highest level of aescp-x ... | 2004 | 15373808 |
| the anterior stomach of larval mosquitoes (aedes aegypti): effects of neuropeptides on transepithelial ion transport and muscular motility. | the present investigation studied the influence of a number of neuropeptides on semi-open preparations of the isolated and perfused anterior stomach of larval aedes aegypti. effects of peptides were observed on the lumen negative transepithelial voltage (vte) that is present with serotonin in the bath; this voltage most likely reflects active hco3- secretion involved in alkalization of the larval anterior stomach. the five different a. aegypti allatostatins (allatostatin a 1-5) all affected vte ... | 2004 | 15371480 |
| [larvicidal activity of tannins isolated of magonia pubescens st. hil. (sapindaceae) against aedes aegypti (diptera, culicidae)]. | phytochemical study of the larvicidal fractions which were carried out for the first time, isolated of the magonia pubescens, monitored by the study of efficacy against the 3rd larval instar of aedes aegypti, in the search of alternatives for the control of that mosquito and to obtain structures susceptible to chemical improving of the activity for the synthetic via of other derived. the fractions with biological activity were monitored chemically through chromatography of thin layer, using as r ... | 2006 | 15361956 |
| utilization of pre-existing energy stores of female aedes aegypti mosquitoes during the first gonotrophic cycle. | pre-existing energy reserves may play an important role in regulating the utilization of blood meal proteins in female anautogenous mosquitoes. determining the fate of reserves derived from the sugar meal and larval food during the first gonotrophic cycle would help to elucidate the relative contributions of larval and adult nutrition to survival and reproduction. we measured the allocation of pre-blood-meal reserves to egg production or energy production during the first gonotrophic cycle by us ... | 2004 | 15350611 |
| intriguing olfactory proteins from the yellow fever mosquito, aedes aegypti. | four antennae-specific proteins (aaegobp1, aaegobp2, aaegobp3, and aaegasp1) were isolated from the yellow fever mosquito, aedes aegypti and their full-length cdnas were cloned. rt-pcr indicated that they are expressed in female and, to a lesser extent, in male antennae, but not in control tissues (legs). aaegobp1 and aaegobp3 showed significant similarity to previously identified mosquito odorant-binding proteins (obps) in cysteine spacing pattern and sequence. two of the isolated proteins have ... | 2004 | 15338030 |
| mosquito larvicidal properties of essential oil of an indigenous plant, ipomoea cairica linn. | laboratory bioassay of the essential oil extracted from an indigenous plant, ipomoea cairica, commonly known as 'railway creeper', was carried out against the larvae of four vector species of mosquitoes in order to evaluate its mosquito larvicidal effect. bioassay test revealed that the essential oil of the plant possess remarkable larvicidal properties as it could induce 100% mortality in the larvae of culex tritaeniorhynchus (100 ppm), aedes aegypti (120 ppm), anopheles stephensi (120 ppm), an ... | 2004 | 15329451 |
| evaluation of the colchicine-like activity of gloriosa superba-extracted fractions for mosquito (diptera: culicidae) cytogenetic study. | four fractions of gloriosa superba l., i.e., hexane fraction, dichloromethane fraction 1, dichloromethane fraction 2, and methanol fraction, were investigated for colchicine-like activity using a mosquito cytogenetic assay. the results revealed that the latter three fractions yielded promisingly high colchicine-like activity, whereas the hexane fraction yielded very low activity compared with 1% colchicine in a 0.85% sodium chloride solution. the metaphase rates and average number of metaphase c ... | 2004 | 15311459 |
| food as a limiting factor for aedes aegypti in water-storage containers. | an understanding of the ecological factors that regulate natural populations of aedes aegypti mosquitoes can improve control and reduce the incidence of dengue (df) and dengue hemorrhagic fever (dhf) in tropical areas. we investigated whether immature ae. aegypti in water-storage containers from an urban area were under food limitation. we used starvation resistance (number of days alive without food) as an indicator of the feeding history in third-instar ae. aegypti larvae. resistance to starva ... | 2004 | 15266737 |
| repellency of aromatic medicinal plant extracts and a steam distillate to aedes aegypti. | the repellent activity of methanol extracts from 23 aromatic medicinal plant species and a steam distillate against female blood-starved aedes aegypti was examined in the laboratory by skin test and compared with that of n,n-diethyl-m-toluamide (deet). responses varied according to plant species. at a dose of 0.1 mg/cm2, the repellency of extracts of cinnamomum cassia bark (91%), nardostachys chinensis rhizome (81%), paeonia suffruticosa root bark (80%), and cinnamomum camphora steam distillate ... | 2004 | 15264623 |
| [social representations of the relationships between plant vases and the dengue vector]. | to investigate how houseplant 'caretakers' represent the relationship between plant vases and breeding sites for the dengue vector, in order to reformulate educational policies. | 2004 | 15243671 |
| aedes aegypti genomics. | the mosquito, aedes aegypti, is the primary, worldwide arthropod vector for the yellow fever and dengue viruses. as it is also one of the most tractable mosquito species for laboratory studies, it has been and remains one of the most intensively studied arthropod species. this has resulted in the development of detailed genetic and physical maps for ae. aegypti and considerable insight into its genome organization. the research community is well-advanced in developing important molecular tools t ... | 2004 | 15242713 |
| tn5 as an insect gene vector. | the purpose of this study was to explore alternatives to insect-derived transposable elements as insect gene vectors with the intention of improving existing insect transgenesis methods. the mobility properties of the bacterial transposon, tn5, were tested in mosquitoes using a transient transposable element mobility assay and by attempting to create transgenic insects. tn5 synaptic complexes were assembled in vitro in the absence of mg(2+) and co-injected with a target plasmid into developing y ... | 2004 | 15242711 |
| the mechanism of action of the antidiuretic peptide tenmo adfa in malpighian tubules of aedes aegypti. | the mechanism of action of tenebrio molitor antidiuretic factor 'a' (tenmo adfa) was explored in isolated malpighian tubules of aedes aegypti. in the ramsay assay of fluid secretion, tenmo adfa (10(-9) mol l(-1)) significantly inhibited the rate of fluid secretion from 0.94 nl min(-1) to 0.44 nl min(-1) without significant effects on the concentrations of na+, k+ and cl- in secreted fluid. in isolated perfused tubules, tenmo adfa had no effect on the transepithelial voltage (vt) and resistance ( ... | 2004 | 15235016 |
| critical period for pupal commitment in the yellow fever mosquito, aedes aegypti. | changes in ecdysteroid levels that lead to commitment of pupal and adult development were studied in the yellow fever mosquito, aedes aegypti. application of juvenile hormone at the time of pupal commitment usually results in delay or blockage of pupal and adult development. with methoprene, a juvenile hormone mimic, the jh sensitive period was found to be within 19 h after ecdysis to the fourth instar, at which time methoprene treatment caused a delay in pupation. consistent with this observati ... | 2004 | 15234627 |
| reduced juvenile hormone synthesis in mosquitoes with low teneral reserves reduces ovarian previtellogenic development in aedes aegypti. | we investigated the relationship among nutritional reserves, previtellogenic ovary development and juvenile hormone (jh) synthesis in aedes aegypti female mosquitoes. by raising larvae under different nutritional regimes, two adult phenotypes (large and small females) were generated, which differed significantly in size at eclosion (measured by wing length). we measured the total amount of protein, lipids and glycogen in newly emerged (teneral) large and small females. teneral reserves were sign ... | 2004 | 15201301 |
| differential modulation of murine host immune response by salivary gland extracts from the mosquitoes aedes aegypti and culex quinquefasciatus. | mosquitoes (diptera: culicidae) are major vectors of numerous infectious agents. compounds in mosquito saliva not only facilitate blood-feeding, but may also have an impact upon the immune system of vertebrate hosts. consequently, the exposure to mosquito saliva may influence pathogen transmission, establishment and disease development. using two medically important vector mosquitoes, aedes aegypti (l.) and culex quinquefasciatus say, we examined the effects of mosquito saliva on immune cells of ... | 2004 | 15189245 |
| in vitro repellency of n,n-diethyl-3-methylbenzamide and n,n-diethylphenylacetamide analogs against aedes aegypti and anopheles stephensi (diptera: culicidae). | seventeen analogs of the repellent compounds n,n-diethyl-3-methylbenzamide (deet) and n,n-diethylphenylacetamide (depa) were evaluated in vitro for repellency against laboratory-reared aedes aegypti (l.) and anopheles stephensi liston mosquitoes by using a modified membrane blood feeding test system. the system was a valuable tool for comparing the effective concentrations of the repellent analogs. additionally, this method used easily controlled test conditions, allowing completion of the many ... | 2004 | 15185946 |
| alkalization of larval mosquito midgut and the role of carbonic anhydrase in different species of mosquitoes. | we have previously demonstrated the involvement of carbonic anhydrase (ca) in the alkalization mechanism of the aedes aegypti larval midgut. in this study, we used hansson's histochemistry to examine the distribution of the enzyme in the midgut of six different species of mosquito larvae (aedes aegypti, aedes albopictus, culex quinquefasciatus, culex nigripalpus, ochlerotatus taeniorhynchus, anopheles quadrimaculatus). additionally, we quantitated ca content in the gastric caeca, anterior and po ... | 2004 | 15171946 |
| variation of genetic expression during development, revealed by esterase patterns in aedes aegypti (diptera, culicidae). | polyacrylamide gel electrophoresis was used to analyze esterase patterns during development of aedes aegypti from the cities of marília and são josé do rio preto (sjrp), brazil. the zymograms showed a total of 23 esterase bands, 22 of which were in the specimens from marília and 19 in those from sjrp. these esterase bands were considered to be the product of 23 alleles distributed tentatively in eight genetic loci. most of the alleles were developmentally regulated. the larval stage expressed th ... | 2004 | 15168721 |
| [effect of temperature on longevity and infection with the nematode strelkovimermis spiculatus (nemata: mermithidae), a parasite of mosquitoes]. | strelkovimermis spiculatus is a common parasite of culicid species in argentina. effect of temperature on longevity and infectivity of juvenile preparasites of s. spiculatus was determined at 4, 10, 20 and 27 degrees c. three containers with 100 ml of dechlorinate water and 300 preparasites (12 hour-old), were placed for each day and temperature, during 40 days (total = 480 containers). survived preparasites were counted on 12 containers per day (three for each temperature). when number of survi ... | 2006 | 15162782 |
| ph tolerances and regulatory abilities of freshwater and euryhaline aedine mosquito larvae. | the ph regulatory abilities of two members of the mosquito tribe aedini, known to have dramatically different saline tolerances, are investigated. the freshwater mosquito aedes aegypti and the euryhaline ochlerotatus taeniorhynchus tolerate very similar ph ranges. both species complete larval development in waters ranging from ph 4 to ph 11, but naïve larvae always die in water of ph 3 or 12. across the ph range 4-11, the hemolymph ph of o. taeniorhynchus is maintained constant while that of a. ... | 2004 | 15159434 |
| differences in the effects of salinity on larval growth and developmental programs of a freshwater and a euryhaline mosquito species (insecta: diptera, culicidae). | the effects of salinity on growth and development of the euryhaline ochlerotatus taeniorhynchus and the freshwater aedes aegypti are compared. o. taeniorhynchus grow larger, and have greater intrinsic growth rates, than a. aegypti. females of each species attain greater mass, take longer to develop, and have greater growth rates than do males. in o. taeniorhynchus, pupal mass, larval stage duration and growth rates (dry mass) increase with salinity, whereas growth rates (wet mass) remain constan ... | 2004 | 15159433 |
| subcellular localization of the mosquito sterol carrier protein-2 and sterol carrier protein-x. | subcellular distribution of aedes aegypti sterol carrier protein-2 (aescp-2) and aescp-x was studied using electron microscopy. in both cultured a. aegypti cells and in the larval midgut, aescp-2 was detected mostly in the cytosol, with some labeling mitochondria and nucleus, but not in membranous vesicles. the widespread distribution of aescp-2 in the midgut epithelium is consistent with its potential lipid transfer function in all phases of cholesterol absorption. in contrast, aescp-x was foun ... | 2004 | 15145982 |
| yellow fever in the americas. | dutch slave traders brought yellow fever to the americas from africa during the mid-seventeenth century. for the next two and a half centuries, the disease terrorized seaports throughout the americas. proof of the mosquito hypothesis was delayed because of two aspects of the disease: patients are viremic only during the first several days of clinical illness, and most mosquitoes require about 2 weeks of viral incubation before becoming infectious. control of aedes aegypti in urban centers failed ... | 2004 | 15145381 |
| the early gene e74b isoform is a transcriptional activator of the ecdysteroid regulatory hierarchy in mosquito vitellogenesis. | in the mosquito aedes aegypti, blood feeding activates vitellogenesis that involves yolk protein precursor (ypp) genes in an insect metabolic tissue, the fat body. vitellogenesis is regulated by the 20-hydroxyecdysone (20e) regulatory hierarchy, in which the ets-domain protein e74 is a key transcriptional regulator. the mosquito aae74 gene encodes two isoforms-aae74a and aae74b. both aae74 isoforms are 20e-inducible early gene products. aae74b reaches its maximal expression at 10(-7)m of 20e, wh ... | 2004 | 15130514 |
| [productivity of aedes albopictus' breeding containers in paraíba valley, brazil]. | to estimate the potential productivity of artificial, permanent, and natural breeding containers of aedes albopictus, potential vector species of dengue. | 2004 | 15122376 |
| temephos resistance in two forms of aedes aegypti and its significance for the resistance mechanism. | aedes aegypti, at the larval stage, has been subjected to the temephos selection in laboratory. the level of temephos resistance was detected in a microplate by biochemical assay using who bioassay technique. the major enzyme-based resistance mechanisms involved in temephos resistance include elevated nonspecific esterase, oxidase and insensitive acetylcholinesterase. after 19 generations of temephos selection, the selected group showed resistance ratios of 4.64 and 16.92, when compared with a n ... | 2003 | 15115088 |
| the transepithelial voltage of the isolated anterior stomach of mosquito larvae (aedes aegypti): pharmacological characterization of the serotonin-stimulated cells. | the lumen-negative transepithelial voltage (v(te)) of the isolated and perfused anterior stomach of mosquito larvae (aedes aegypti) was studied with a 'semi-open' preparation in which one end of the gut was ligated onto a perfusion pipette and the other end remained open to the bath. all experiments were performed with serotonin-stimulated preparations. v(te) was abolished after addition of 2.5 mmol l(-1) dinitrophenol and depended on the presence of cl(-). na(+) substitution experiments showed ... | 2004 | 15107433 |
| [a simulation model for the control of the aedes aegypti, the mosquito vector of dengue and yellow fever, by the crustacean mesocyclops spp]. | a simulation model is presented to show the predation dynamics of mesocyclops spp. over aedes aegypti. | 2006 | 15106875 |
| [evaluation of the inhibiting activity of the diflubenzuron on the ecdysis of larvae of aedes aegypti (linnaeus, 1762) (diptera, culicidae)]. | the inhibiting activity of diflubenzuron on the ecdysis of aedes aegypti larvae was evaluated, with a view to using this product in mosquito control. this study also aimed to determine the interaction between this product, the type of artificial containers and the susceptibility of the mosquito. bioassays were carried out in the backyard of a residence, using seven kinds of artificial habitats: tires, glass, concrete roofing, cans, plastic containers, cement and pottery. in each kind of artifici ... | 2013 | 15094897 |
| metabolic fate of [14c]-labeled meal protein amino acids in aedes aegypti mosquitoes. | we developed a method to follow the metabolic fate of [(14)c]-labeled euglena gracilis protein amino acids in aedes aegypti mosquitoes under three different adult nutritional regimes. quantitative analysis of blood meal protein amino acid metabolism showed that most of the carbon of the amino acids was either oxidized to co(2) or excreted as waste. under the three different adult nutritional regimes, no significant differences in the metabolism of amino acids were found, which indicated that the ... | 2004 | 15081827 |
| mechanisms of k+ transport across basolateral membranes of principal cells in malpighian tubules of the yellow fever mosquito, aedes aegypti. | the mechanisms of k(+) entry from the hemolymph into principal cells of malpighian tubules were investigated in the yellow fever mosquito, aedes aegypti. the k(+) channel blocker ba(2+) (5 mmol l(-1)) significantly decreased transepithelial (tep) fluid secretion (v(s)) from 0.84 nl min(-1) to 0.37 nl min(-1) and decreased the k(+) concentration in secreted fluid from 119.0 mmol l(-1) to 54.3 mmol l(-1) with no change in the cl(-) concentration. even though the na(+) concentration increased signi ... | 2004 | 15073198 |
| field validation of aedes aegypti (diptera: culicidae) age estimation by analysis of cuticular hydrocarbons. | in previous studies, we developed linear regression models to age-grade female aedes aegypti l. reared and maintained under controlled laboratory conditions. the models were based on temporal differences between two cuticular hydrocarbons, pentacosane (c25h52) and nonacosane (c29h60), which were extracted from ae. aegypti legs and analyzed by gas-liquid chromatography. these initial models predicted adult female age up to 165 dd (12-15 calendar d at 28 degrees c). the age of older mosquitoes, ho ... | 2004 | 15061283 |
| laboratory evaluation of methanolic extract of atlantia monophylla (family: rutaceae) against immature stages of mosquitoes and non-target organisms. | methanolic extracts of the leaves of atlantia monophylla (rutaceae) were evaluated for mosquitocidal activity against immature stages of three mosquito species, culex quinquefasciatus, anopheles stephensi, and aedes aegypti in the laboratory. larvae of cx. quinquefasciatus and pupae of an. stephensi were found more susceptible, with lc50 values of 0.14 mg/l and 0.05 mg/l, respectively. insect growth regulating activity of this extract was more pronounced against ae. aegypti, with ei50 value 0.00 ... | 2004 | 15057359 |
| [risk perception and strategies for mass communication on dengue in the americas]. | dengue is clearly a very serious public health problem. in the americas the number of dengue cases has been increasing since the 1960s, and outbreaks of the disease have been occurring more frequently. furthermore, the density of infestation with the disease vector, the aedes aegypti mosquito, is high in the americas. the general strategy for preventing and controlling dengue and dengue hemorrhagic fever is based on promoting behavior changes that lead to incorporating the community in controlli ... | 2004 | 15030659 |
| an immune signalling kinase aamek3 from mosquitoes: cdna cloning and characterization. | in mammals, the mitogen-activated protein (map) kinase pathway is one of the four major signalling systems that respond to stress and inflammatory stimuli. a full-length cdna corresponding to aedes aegypti map kinase kinase 3 (aamek3) was cloned and sequenced. it is 1.7 kb and contains an open reading frame of 334 amino acids and eleven conserved kinase domains, including signatures of a putative serine/threonine kinase active site and an atp binding site. the messenger (mrna) and protein expres ... | 2003 | 14986920 |
| three regulatory regions of the aedes aegypti glutamine synthetase gene differentially regulate expression: identification of a crucial regulator in the first exon. | aedes aegyptiglutamine synthetase (gs) is expressed constitutively at various developmental stages and its relative mrna abundance increases in the midgut following blood feeding in support of the biosynthesis of chitin, a component of the peritrophic matrix. to understand the regulation of gs expression better, gs-luciferase reporter fusion genes were constructed and analysed in transiently transfected c6/36 cells. these studies have identified three gs regions: gs-a, -b and -c (c1, c2) that ar ... | 2003 | 14986918 |
| in vitro expression and pharmacology of the 5-ht7-like receptor present in the mosquito aedes aegypti tracheolar cells and hindgut-associated nerves. | we have previously reported the cloning of a 5-hydroxytryptamine receptor (aedes 5-ht7-like receptor) from adult aedes aegypti. for functional expression of the aedes 5-ht7-like receptor, cho-k1 cells were stably transfected with a receptor expression construct, pc5-ht7. the aedes 5-ht7-like receptor positively coupled to gs protein, increasing intracellular camp in response to 5-ht; adenylyl cyclase activity was induced in a concentration-dependent, saturable manner. only 5-ht, and not octopami ... | 2003 | 14986917 |
| mucigerin, a new coumarin from calophyllum mucigerum (guttiferae). | our recent studies on the stem bark of calophyllum mucigerum (guttiferae) have yielded a new coumarin mucigerin, a prenylated xanthone cudraxanthone c and the common steroidal triterpenes friedelin and stigmasterol. structural elucidations of these compounds were achieved using 1h nmr, 13c nmr, dept, cosy, hetcor and hmbc experiments while ms gave the molecular masses. cytotoxic assays using cem-ss cell line (t-lymphoblastic leukemia) on the crude extracts of the stem bark indicated some activit ... | 2004 | 14984084 |
| biochemical and cytoimmunological evidence for the control of aedes aegypti larval trypsin with aea-tmof. | trypsin and chymotrypsin-like enzymes were detected in the gut of aedes aegypti in the four larval instar and pupal developmental stages. although overall the amount of trypsin synthesized in the larval gut was 2-fold higher than chymotrypsin, both enzymes are important in food digestion. feeding aea-trypsin modulating oostatic factor (tmof) to ae. aegypti and culex quinquefasciatus larvae inhibited trypsin biosynthesis in the larval gut, stunted larval growth and development, and caused mortali ... | 2004 | 14981657 |
| [domestic water reservoir as breeding site of aedes aegypti]. | | 2004 | 14963555 |
| the impact of selective oviposition and infection with plagiorchis elegans on aedes aegypti pre-imago population dynamics at optimal food availability. | progressive changes in the attraction of waters harboring pre-imago populations of aedes aegypti exposed to different levels of the entomopathogenic digenean plagiorchis elegans to ovipositing conspecific females were assessed under conditions of optimal food availability. the impact of ovipositional preference and parasitic infection on population structure and development was investigated. probabilities that larvae progress from one stage to the next or die within 24 h were calculated for all ... | 2003 | 14765660 |
| use of a geographic information system for defining spatial risk for dengue transmission in bangladesh: role for aedes albopictus in an urban outbreak. | we used conventional and spatial analytical tools to characterize patterns of transmission during a community-wide outbreak of dengue fever and dengue hemorrhagic fever in dhaka, bangladesh in 2000. a comprehensive household-level mosquito vector survey and interview was conducted to obtain data on mosquito species and breeding as well as illness consistent with dengue. clusters of dengue illnesses and high-density vector populations were observed in a distinct sector of the city. dengue cluster ... | 2003 | 14740881 |
| laboratory and field evaluation of novaluron, a new acylurea insect growth regulator, against aedes aegypti (diptera: culicidae). | novaluron, a new chitin synthesis inhibitor type of insect growth regulator, was evaluated in the laboratory and field against larvae of the mosquito aedes aegypti (l.). in the laboratory, the technical material showed a high level of activity against 2nd and 4th instar larvae. the inhibition of emergence (ie) was 100% at concentrations of 0.25 to 1.0 microg/l. second instars were slightly more susceptible than fourth instars. at the high concentration (1 ppb), most of the mortality occurred in ... | 2003 | 14714674 |
| laboratory evaluation of 18 repellent compounds as oviposition deterrents of aedes albopictus and as larvicides of aedes aegypti, anopheles quadrimaculatus, and culex quinquefasciatus. | among 18 experimental skin repellent compounds tested at five concentrations in the laboratory as oviposition deterrents against aedes albopictus. 12 compounds showed significant activity, with median effective dose values ranging from 0.005 to 0.052%. the test compounds also were evaluated in the laboratory as larvicides against laboratory-reared 4th instars of aedes aegypti, anopheles quadrimaculatus, and culex quinquefasciatus with the same concentrations employed for the oviposition deterren ... | 2003 | 14710743 |
| effects of leucokinin-viii on aedes malpighian tubule segments lacking stellate cells. | the diuretic peptide leucokinin is known to increase fluid secretion in malpighian tubules of the yellow fever mosquito aedes aegypti by increasing a transepithelial cl(-) conductance. the present study sought to examine whether stellate cells provided this transepithelial conductance in aedes malpighian tubules as they do in drosophila malpighian tubules. aedes malpighian tubule segments with and without stellate cells were perfused in vitro for measurements of the transepithelial voltage (v(t) ... | 2004 | 14691099 |
| relative efficacy of five synthetic pyrethroids against four vector mosquitoes, anopheles culicifacies, anopheles stephensi, culex quinquefasciatus and aedes aegypti. | | 2013 | 14686108 |
| [dengue] | objective: to review the literature about epidemiological, clinical and preventive aspects of hemorrhagic dengue fever in children, and to provide brazilian pediatricians with an update on dengue methods: we reviewed medline and classic texts.results: dengue is an arbovirus disease, transmitted by aedes aegypti, a mosquito that feeds indoors and is disseminated in brazil. its clinical presentation ranges from asymptomatic forms to acute disease with fever, myalgia, headache and rash. evolution w ... | 1999 | 14685479 |
| methoprene interferes with mosquito midgut remodeling during metamorphosis. | many juvenile hormone analogs interfere with insect metamorphosis, a property that makes them useful in insect control and as investigatorial tools with which to study metamorphic mechanisms. we report that one such analog, methoprene, interferes with mosquito metamorphic midgut remodeling. methoprene treated aedes aegypti (l.), aedes albopictus (skuse), and culex quinquefasciatus (say) fourth instars pupate, but the pupal midguts are morphologically similar to larval midguts. the degree of midg ... | 2003 | 14680117 |
| learning and memory in the mosquito aedes aegypti shown by conditioning against oviposition deterrence. | oviposition site choice by female mosquitoes, aedes aegypti (l) (diptera: culicidae), was affected by rearing them in water treated with 0.016% of the repellent mozaway trade mark containing citronella and neem. given a choice between a bowl of repellent-treated and a bowl of untreated water, ae. aegypti reared in untreated water strongly preferred to oviposit on the clean water (93-98%) instead of repellent-treated water. this demonstrates effective deterrence of oviposition by dilute mozaway t ... | 2003 | 14651662 |
| sex-specific reaction norms to intraspecific larval competition in the mosquito aedes aegypti. | as the relationship between a given life-history trait and fitness is not necessarily the same for the two sexes, an 'intersexual ontogenetic conflict' may arise. we analysed the phenotypic reaction to intraspecific larval competition of the mosquito, aedes aegypti, asking: (i) do both sexes pay the cost of competition with the same life-history traits and are they equal competitors? (ii) is there a specific cost of competition beyond sharing food resources? we found that competition incurs a sp ... | 2003 | 14632235 |
| creb isoform represses yolk protein gene expression in the mosquito fat body. | in mosquitoes, the steroid 20-hydroxyecdysone (20e) is the main regulator of yolk protein precursor (ypp) gene expression. however, peptide hormones have also been implicated. to investigate involvement of the camp-mediated signal-transduction cascade in regulation of mosquito vitellogenic events, we cloned an aedes aegypti camp response element binding protein (aacreb). the aacreb contained the domains characteristic to members of the camp response element binding protein (creb) family of trans ... | 2003 | 14615059 |
| chitinolytic activities in the gut of aedes aegypti (diptera:culicidae) larvae and their role in digestion of chitin-rich structures. | mosquito larvae are believed to be capable of digesting chitin, an insoluble polysaccharide of n-acetylglucosamine, for their nutritional benefit. studies based on physiological and biochemical assays were conducted in order to detect the presence of chitinase activities in the gut of the detritus-feeding aedes aegypti larvae. larvae placed for 24 h in suspensions of chitin azure were able to digest the ingested chitin. semi-denaturing page using glycol chitin and two fluorogenic substrate analo ... | 2003 | 14613799 |
| activity of the corpora allata of adult female aedes aegypti: effects of mating and feeding. | the synthesis of juvenile hormone iii (jh iii) by the isolated corpora allata (ca) of aedes aegypti adult female was studied using an in vitro radiochemical assay. we dissected the corpora allata-corpora cardiaca (ca-cc) complex attached to a piece of aorta. the complex was left connected to the intact head capsule to facilitate the visualization and transfer of the glands. a linear increase in the cumulative amount of biosynthesized jh iii was found for at least the first 6 h of incubation; app ... | 2003 | 14599502 |
| synergistic attraction of aedes aegypti (l.) to binary blends of l-lactic acid and acetone, dichloromethane, or dimethyl disulfide. | kairomones produced by humans provide female anthropophilic mosquitoes with vital cues used in host-seeking for a blood meal. these chemicals are emanated primarily by the skin and provide the mosquitoes a means to orient themselves to humans at a relatively close range. chemical studies of these emanations have provided new ideas for the formulation of attractant blends. we report mosquito attraction responses for three binary blends and their separate components. the blends are comprised of l- ... | 2003 | 14596278 |
| effects of a brassinosteroid analogue to mosquito larvae. | many plant compounds are able to modulate herbivore growth and reproduction by directly interacting with steroid hormones systems. in insects, several classes of phytochemicals, including brassinosteroids and related substances, interfere with molting and reproduction. the effects of the molting-hormone antagonist pesticide di-31, a brassinosteroid analogue, on aedes aegypti mosquito larvae were tested in two different exposure scenarios. after static exposure of first-instar larvae, the calcula ... | 2003 | 14575682 |
| [household water reservoirs and control of aedes aegypti]. | water reservoirs for domestic use are important sites for the development of aedes aegypti. in poor areas, these reservoirs are often located outdoors upon flat rooftops. in modern architecture buildings, however, the same is seen even in high-class condominiums. special attention should be given to these modern architectural constructions during entomological surveillance. | 2003 | 14569348 |
| [scanning electron microscopy of eggs of haemagogus leucocelaenus (diptera: culicidae)]. | to observe morphological details of the eggs of haemagogus (conopostegus) leucocelaenus, seen for the first time via scanning electron microscopy (sem), with morphometric analysis of the main structures. | 2003 | 14569344 |
| 1,5-disubstituted imidazoles inhibit juvenile hormone biosynthesis by the corpora allata of the mosquito aedes aegypti. | we investigated the effect of fifteen 1,5-disubstituted imidazoles (1,5-dis) on juvenile hormone iii (jh iii) and methyl farnesoate (mf) biosynthesis by the corpora allata (ca) of the mosquito aedes aegypti in vitro. four compounds (th-35, th-83, th-62 and th-28) significantly decreased jh biosynthesis in the ca dissected from 3-day old sugar-fed females. the decrease of jh synthesis was not always associated with increased mf. th-30 and th-83 increased mf levels, while th-85 and th-61 significa ... | 2003 | 14568578 |
| blood meal induces global changes in midgut gene expression in the disease vector, aedes aegypti. | blood feeding is an essential developmental process for many arthropods and plays a significant role in disease transmission. understanding physiological responses in the midgut is important because it is the primary site of blood meal digestion and pathogenic infection. processes that occur in the midgut in response to a blood meal have been studied but are poorly understood. here, we use cdna microarrays to examine midgut gene expression on a global level in response to blood feeding to assist ... | 2003 | 14563362 |
| effect of rotenoids from the seeds of millettia dura on larvae of aedes aegypti. | a crude chloroform extract of seeds of millettia dura dunn (leguminosae) showed high activity (lc50 = 3.5 microg ml(-1) at 24 h) against second-instar larvae of the mosquito, aedes aegypti l (diptera: culicidae). the rotenoids, deguelin and tephrosin, isolated from the seeds of this plant also showed potent activities, with lc50 values of 1.6 and 1.4 microg ml(-1) at 24 h, respectively. the related rotenoids millettone and millettosin were inactive at 20 microg ml(-1). saturation at the b/c ring ... | 2003 | 14561074 |
| mosquito vector control and biology in latin america--an eleventh symposium. | the 11th annual latin american symposium presented by the american mosquito control association (amca) was held as part of the 67th annual meeting in dallas, tx, in february 2001. the principal objective, as for the previous 10 symposia, was to promote participation in the amca by vector control specialists, public health workers, and academicians from latin america. this publication includes summaries of 45 presentations that were given orally in spanish or presented as posters by participants ... | 2001 | 14529084 |
| comparative toxicity of permethrin- and bifenthrin-treated cloth fabric for anopheles farauti and aedes aegypti. | in this laboratory study, we applied 3 formulations of permethrin (peregin 500 ec, dragnet 500 ec, and dragnet 100 me) and 2 of bifenthrin (biflex 10 me and talstar 80 sc) to swatches of australian military shirt fabric. the knockdown and mortality of anopheles farauti and aedes aegypti after exposure to treated fabrics were compared. the mortality of an. farauti exposed to permethrin-treated swatches for 3 min in world health organization test kits was 94.2-100% after initial treatment and fell ... | 2003 | 14524552 |
| laboratory evaluation of toxicity of 16 insect repellents in aerosol sprays to adult mosquitoes. | sixteen commercial insect repellents (6 botanical and 10 synthetic organic products) in spray formulations were evaluated in the laboratory for adult knockdown (kd) and mortality of laboratory-reared female aedes aegypti, aedes albopictus, and anopheles quadrimaculatus. all tested products produced significant posttreatment kd and 24 h mortality of all 3 mosquito species. in our study, the synthetic organic repellents induced faster kd and kd of higher magnitude in adult mosquitoes than the bota ... | 2003 | 14524551 |
| an adulticidal sticky ovitrap for sampling container-breeding mosquitoes. | the efficacy of a standard ovitrap and an ovitrap featuring an internal wall covered by a polybutylene adhesive was compared in field studies in cairns, australia. the sticky ovitrap was as effective as the standard ovitrap in detecting aedes aegypti, with 67.5% and 64% of traps positive for ae. aegypti, respectively. significantly higher numbers of ae. aegypti were collected by traps set outside rather than inside premises. sticky ovitraps also readily collected ochlerotatus notoscriptus and, e ... | 2003 | 14524545 |
| quantitative evaluation of funnel traps for sampling immature aedes aegypti from water storage jars. | the trend is increasing to incorporate assessments of abundance into surveys for immature aedes aegypti to identify the most important types of containers that should be targeted for control. in this study, we examined whether funnel traps could be used to sample immature ae. aegypti from water storage jars ranging in size from 0.28-m diameter (30 liters) to 0.52-m diameter (150 liters). the effects of jar size and duration of funnel trap sampling were investigated and a set of calibration facto ... | 2003 | 14524543 |
| the peritrophic matrix limits the rate of digestion in adult anopheles stephensi and aedes aegypti mosquitoes. | the peritrophic matrix (pm) is a chitin-containing acellular sheath that surrounds the blood meal and separates the food bolus from the midgut epithelium. intense molecular traffic through the pm occurs during digestion. digestive enzymes secreted by the midgut epithelium must traverse the pm to reach their substrates in the food bolus, and digestion products must cross the pm in the opposite direction to be absorbed by the epithelial cells. here we report that the pm limits the rate of digestio ... | 2003 | 14511821 |
| transport mechanisms of diuresis in malpighian tubules of insects. | we have studied malpighian tubules of aedes aegypti using a variety of methods: ramsay fluid secretion assay, electron probe analysis of secreted fluid, in vitro microperfusion and two-electrode voltage clamp. collectively, these methods have allowed us to elucidate transepithelial transport mechanisms under control conditions and in the presence of diuretic peptides. mosquito natriuretic peptide (mnp), a corticotropin-releasing factor (crf)-like diuretic peptide, selectively increases transepit ... | 2003 | 14506220 |
| gene vector and transposable element behavior in mosquitoes. | the development of efficient germ-line transformation technologies for mosquitoes has increased the ability of entomologists to find, isolate and analyze genes. the utility of the currently available systems will be determined by a number of factors including the behavior of the gene vectors during the initial integration event and their behavior after chromosomal integration. post-integration behavior will determine whether the transposable elements being employed currently as primary gene vect ... | 2003 | 14506218 |
| insecticide susceptibility status of aedes aegypti to ddt and dieldrin in desert and non-desert parts of rajasthan. | seasonal prevalence and insecticide susceptibility tests were carried out on the adults of aedes aegypti, the vector of dengue and dengue hemorrhagic fever(df/dhf), in three desert (bikaner, jaisalmer and jodhpur) and three non-desert (alwar, ajmer and jaipur) districts of rajasthan. among the three species viz. ae. aegypti, ae. vittatus and ae. w-albus encountered during the survey the former was the most prevalent species. studies were carried out both in rural and urban areas against differen ... | 2003 | 12974421 |
| larvicidal activity of the essential oil from lippia sidoides cham. against aedes aegypti linn. | the aim of this work was to study the larvicidal activity of lippia sidoides essential oil against aedes aegypti larvae. the essential oil and its hydrolate (saturated solution of essential oil in water) were obtained by vapor extraction and their chemical composition determined by gl-chromatography coupled to mass spectroscopy. bioassays were run with the essential oil, pure and diluted hydrolate and with their main constituents thymol and carvacrol. the results obtained showed that l. sidoides ... | 2003 | 12937776 |
| [larval susceptibility of aedes aegypti and culex quinquefasciatus populations to chemical insecticides]. | to evaluate the susceptibility to chemical insecticides of culex quinquefasciatus and aedes aegypt larvae from areas subjected to control treatments or not. | 2003 | 12937715 |
| regulation of tight junction permeability with switch-like speed. | the case is made that tight junctions can undergo large reversible conductance changes in a matter of seconds and yet preserve their permselectivity. the diuretic peptide leucokinin transforms (renal) malpighian tubules of the yellow fever mosquito from a moderately tight epithelium to a leaky epithelium by increasing the chloride-conductance of the paracellular shunt pathway. the nine-fold increase in the paracellular chloride-conductance brings about a non-selective stimulation of transepithel ... | 2003 | 12920403 |
| post-integration behavior of a mos1 mariner gene vector in aedes aegypti. | the post-integration behavior of insect gene vectors will determine the types of applications for which they can be used. transposon mutagenesis, enhancer trapping, and the use of transposable elements as genetic drive systems in insects requires transposable elements with high rates of remobilization in the presence of transposase. we investigated the post-integration behavior of the mos1 mariner element in transgenic aedes aegypti by examining both germ-line and somatic transpositions of a non ... | 2003 | 12915177 |
| a technique for preparing polytene chromosomes from aedes aegypti (diptera, culicinae). | polytene chromosome preparations were obtained from larval, pupal and adult female malpighian tubules of aedes aegypti. the malpighian tubules of the pupae (0-4 h old) from larvae reared at 20 c provided the best cytogenetic analysis. the interaction of nucleic acids and proteins that influence the spreading of the chromosomes could be reduced with the preparation technique of the sheets submitted to a stronger treatment starting with the hypotony of tissue and successive bathings with acetic ac ... | 2003 | 12886421 |
| identification, functional characterization and expression of a lat type amino acid transporter from the mosquito aedes aegypti. | we isolated two cdnas from the mosquito aedes aegypti, an l-amino acid transporter (aealat) and a cd98 heavy chain (aeacd98hc). expression of aeacd98hc or aealat alone in xenopus oocyte did not induce amino acid transport activity. however, co-expression of aeacd98hc and aealat, which are postulated to form a heterodimer protein linked through a disulfide bond, showed significant increase in amino acid transport activity. this heterodimeric protein showed uptake specificity for large neutral and ... | 2003 | 12878228 |
| wax moth, galleria mellonella, high density lipophorin receptor: alternative splicing, tissue-specific expression, and developmental regulation. | a lipophorin (lp) receptor cdna from the fat body of galleria mellonella (lepidoptera) was cloned and sequenced. this is the first result in this order, lepidoptera. it showed the pattern of the vldl receptor belonging to the ldl receptor family. sequence homology with other lp receptors in insects, locusta migratoria and aedes aegypti, was 70 and 61%, respectively and each domain was highly conserved. polyclonal anti-lp receptor antibody prepared against expressed lp receptor fragment between l ... | 2003 | 12878223 |
| efficacy of two organophosphorus insecticides, reldan & dursban against the larvae of culex quinquefasciatus. | the resistance in vectors to conventional insecticides warrants development of newer insecticides for mosquito control besides use of other counter-measures. the present study was undertaken to test the efficacy of a new and toxicologically safe organophosphorus compound, reldan (chlorpyrifos-methyl) and compare its efficacy with dursban (chlorphyrifos-ethyl) against larvae of different mosquito vectors under laboratory conditions and against culex quinquefasciatus in three larval habitats. | 2003 | 12866823 |
| expression of an aedes aegypti cation-chloride cotransporter and its drosophila homologues. | insects maintain haemolymph homeostasis under different environmental conditions by modulating the concentrations of na+, k+ and cl- ions. one group of proteins involved in ion transport across cell membranes consists of cation-chloride cotransporters that form a family of structurally similar proteins. although much is known about these proteins in mammalian systems, our understanding of them in insects is lacking. the recent sequencing of two insect genomes, drosophila and anopheles, enabled u ... | 2003 | 12864912 |
| comparison of the mosquito-repelling efficacy of methyl neodecanamide (mnda) to that of deet. | the objective of this small pilot study was to assess the mosquito-repelling efficacy of methyl neodecanamide (mnda) relative to n,n-diethyl-3-methylbenzamide (deet) against anopheles stephensi, culex quinquefasciatus, and aedes aegypti under controlled laboratory conditions. in this study, subjects inserted both forearms (sham- and repellent-treated) into a test chamber containing 50 female mosquitoes. the number of mosquitoes landing on or probing each forearm during the 5-minute "forced-choic ... | 2006 | 12858227 |
| population and parity levels of aedes aegypti collected in tucson. | oviposition traps were used to follow changes in the population of aedes aegypti (l.) (diptera:culicidae) in a seven-block area in midtown region of tucson, arizona. about 20,000 eggs were collected over a period from 1 june to 14 october 2000. peak mosquito populations were correlated with the late summer rains. mosquitoes seeking a blood meal were collected and dissected to determine if they had previously fed, i.e. if they were parous. of the 241 females examined, 44% were parous, with a rang ... | 2003 | 12831130 |
| bromeliad-inhabiting mosquitoes in south florida: native and exotic plants differ in species composition. | at several locations in south florida, water impounded by the leaf axils of native or exotic bromeliads was sampled for immature mosquitoes. culex biscaynensis was the most commonly collected mosquito from exotic bromeliads at study sites in southeastern miami-dade county, whereas at nearby sites with native bromeliads, immature wyeomyia mitchellii were more abundant than immature culex biscaynensis. aquatic habitat size and persistence may be important factors favoring culex biscaynensis in exo ... | 2003 | 12831127 |
| physiological bases of mosquito ecology. | the research carried out during more than 30 years in the author's laboratory is briefly reviewed. quantitative analyses of basic physiological processes, such as growth and development, digestion and excretion, oogenesis and fecundity, reserve synthesis and resulting flight-potentials of aedes aegypti were summarized and compared with several other mosquito species, particularly with anopheles. these studies led to the recognition of distinctly different physiological strategies, for which the ... | 2003 | 12831123 |
| does s-methoprene affect oviposition by aedes aegypti in an ovitrap? | we conducted a field study to test if the addition of a pellet of s-methoprene (altosid pellets) to an ovitrap would affect oviposition by aedes aegypti or ochlerotatus notoscriptus. in 2 trials conducted near cairns, queensland, australia, no significant difference (p > 0.05) was found in the number of eggs of ae. aegypti or oc. notoscriptus laid in ovitraps with or without a methoprene pellet. these results suggest the addition of an s-methoprene pellet to ovitraps or sentinel tires could be e ... | 2003 | 12825672 |