Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| serum composition of aedes aegypti (diptera: culicidae) larvae and the production of an oviposition repellent are influenced by infection with the entomopathogenic digenean plagiorchis elegans (trematoda: plagiorchiidae), starvation, and crowding. | subjecting aedes aegypti (l.) larvae to conditions that induced the production of oviposition repellency also reduced their wet and dry weights and the concentration of total serum carbohydrates, amino acids, and proteins. thus, infection with metacercariae of the entomopathogenic digenean plagiorchis elegans (rudolphi), starvation for 7 d, or crowding for 2 d reduced larval dry weights by as much as 32, 20, and 23%, respectively, and wet weights by 20, 14, and 11%, respectively. total serum car ... | 1998 | 9538578 |
| expression and rapid purification of an aedes aegypti salivary allergen by a baculovirus system. | mosquito salivary proteins cause allergic reactions in humans. recombinant salivary allergens will facilitate both the diagnosis and immunotherapy of mosquito allergy. the aed a 1, a 68-kd apyrase in the saliva of aedes aegypti, has been demonstrated to be an allergen which binds to the ige of mosquito-allergic subjects. the baculovirus expression vector pbluebachis c equipped with an n-terminal histidine tag was used to express the aed a 1 protein. the cdna coding for the 3' significant portion ... | 1998 | 9531167 |
| identification of a steroidogenic neurohormone in female mosquitoes. | in the female mosquito, aedes aegypti, neurohormones are released from the brain in response to a blood meal and stimulate the ovaries to secrete ecdysteroid hormones, which modulate yolk protein synthesis in the fat body. neuropeptides with this bioactivity were isolated from head extracts, and partial sequences from these peptides when aligned gave a 31-residue sequence at the amino terminus. oligonucleotide primers for this sequence were used to amplify with the polymerase chain reaction a ge ... | 1998 | 9461584 |
| effect of heavy metals on aedes aegypti (diptera: culicidae) larvae. | studies were conducted to determine the biological effects of heavy metals on the development of aedes aegypti. embryos immersed in 32 ppm cu or 5 ppm cd did not hatch. the arrest of hatching was in part reversible by removal of the heavy metals. the mortality rate of third-instar larvae exposed to heavy metals for 24 h was metal and dose dependent; the 50% lethal concentration (lc50) endpoints were 3.1, 16.5, and 33 ppm for hg, cd, and cu, respectively. interestingly, a proportion of aedes aegy ... | 1998 | 9515074 |
| isolation of the v-atpase a and c subunit cdnas from mosquito midgut and malpighian tubules. | using conserved amino acid sequences for the design of oligonucleotide primers, we isolated cdna clones for two subunits of the v-atpase from the midgut and malpighian tubules of aedes aegypti larvae. the 3.1 kb cdna of the a subunit of the peripheral catalytic v1 sector codes for a protein of 68.6 kda. the protein contains conserved motifs, including an atp/gtp binding site, found in all other a subunits. southern analysis using the a subunit as a probe suggests the presence of only a single co ... | 1998 | 9397516 |
| natriuretic and depolarizing effects of a stable fly (stomoxys calcitrans) factor on malpighian tubules. | a two-step hplc purification procedure resulted in a factor from the stable fly that depolarizes the lumen-negative transepithelial voltage (v(t)) of the adult stable fly malpighian tubule. when applied to tubules of the female mosquito, aedes aegypti, this factor partially mimics the electrophysiological actions of the mosquito natriuretic factor (mnf). it also selectively increases active transepithelial na transport by the mosquito malpighian tubule. the blood meal causes a transient increase ... | 1997 | 12770470 |
| breeding habitats of aedes aegypti (l) and aedes. albopictus (skuse) in villages of barru, south sulawesi, indonesia. | the breeding habitats of the dengue vector, aedes aegypti and aedes albopictus, were studied using larval collection method inside and outside houses in 6 villages of barru, south sulawesi, indonesia from july 1994 to august 1995. aedes aegypti was the dominant species, being abundant indoors especially in the coastal areas. aedes albopictus was breeding primarily in outdoor containers in the hill and mountain areas. earthen jar was the most common breeding habitat of aedes aegypti in all villag ... | 1997 | 9656413 |
| effect of sublethal dosages of malathion on the oral susceptibility of aedes aegypti to dengue-2 virus infection. | 1997 | 9561626 | |
| survey of cyclopids (crustacea, copepoda) in brazil and preliminary screening of their potential as dengue vector predators. | cyclopid copepods are known to be good mosquito controllers, specially as regards the larvae of the dengue vectors aedes aegypti and ae. albopictus. | 1997 | 9515258 |
| hemagglutinins in mosquitoes and their role in the immune response to brugia malayi (filarioidea:nematoda) larvae. | hemagglutinins were determined in six species of mosquitoes that are susceptible and refractory to brugia malayi (filarioidea: nematoda). high titers of hemagglutinins were found in the salivary gland extract and in the body fluid of a completely refractory species, aedes taeniorhynchus, and in partially refractory species, anopheles quadrimculatus but low levels of hemagglutinins were also present in the body fluid of aedes aegypti (black-eye, liverpool strain), a susceptible species. hemagglut ... | 1997 | 9505436 |
| [atypical activity rhythm in aedes aegypti in a sub-sudanian savannah zone of côte d'ivoire]. | ae. aegypti is vector of many arboviruses of medical and/or economical importance. it transmits the yellow fever, dengue and chikungunya viruses. small water collections constitute its potential breeding sites. three different populations of ae. aegypti are known in africa: a selvatic population breeding in tree holes and rock holes, a population breeding in discarded containers in peri-domestic area and a third population which breeds in drinking water containers and various receptacles inside ... | 1997 | 9507772 |
| reduced transdermal absorption of n,n-diethyl-m-toluamide from a new topical insect repellent formulation. | extensive absorption of the topical insect repellent n, n-diethyl-m-toluamide (deet) causes systemic and local toxicities. this report describes the preparation and characterization of a new insect repellent formulation (fa), a peg-polyacrylic acid polymer system, for its deet release, in vitro skin permeation, and in vivo transdermal absorption properties; and for its relative repellency against aedes aegypti mosquitoes. deet release and skin permeation were studied in franz diffusion cells. de ... | 1997 | 9552429 |
| dengue--a re-emerging infectious disease in singapore. | despite its well-established integrated nationwide aedes mosquito control programme which incorporates source reduction, public health education and law enforcement, singapore has not been spared from the regional resurgence of dengue. the disease incidence has been increasing from 9.3 per 100,000 in 1988 to 102.7 per 100,000 in 1996 at the time when the aedes house index (hi) has dropped to around 1% from > 25% in the 1960s. majority of the cases reported from 1990 to 1996 were dengue fever (df ... | 1997 | 9494676 |
| parasites of the asian tiger mosquito and other container-inhabiting mosquitoes (diptera:culicidae) in northcentral florida. | seven microorganisms including 4 protozoans, 2 fungi, and a bacterium infected aedes albopictus (skuse) larvae collected from 12 counties in northecentral florida. ae albopictus and 14 other species of mosquitoes were collected from tires, flower-holding vases in cemeteries, other types of artificial containers, and treeholes. ascogregarina taiwanensis (lien & levine) was the most common parasite of ae. albopictus throughout the year. the microsporidium vavraia culicis (weiser) infected aedes ae ... | 1997 | 9103767 |
| 4-deoxyannomontacin and (2,4-cis and trans)-annomontacinone, new bioactive mono-tetrahydrofuran annonaceous acetogenins from goniothalamus giganteus. | 4-deoxyannomontacin (1) and a mixture of (2,4-cis and trans)-annomontacinone (2), new bioactive mono-tetrahydrofuran (thf) gamma-lactone and keto-lactone acetogenins, respectively, as well as five known mono-thf acetogenins [xylomaticin, longifolicin, longicoricin, (2,4-cis and trans)-gigantetrocinone, and (2,4-cis and trans)-gigantetroneninone], were isolated from the bark of goniothalamus giganteus (annonaceae) by activity-directed fractionation using the brine shrimp lethality test (bst). the ... | 1997 | 9113333 |
| influence of plant abundance on nectar feeding by aedes aegypti (diptera: culicidae) in southern mexico. | the availability of flowering plants affected the sugar feeding rates of female aedes aegypti (l.) in 4 areas of a small city in southern mexico. the proportion of mosquitoes containing sugar varied from 8 to 21% in 4 areas in direct relation to blooming plant abundance. human density was similar in the 4 areas (range, 3.9-5.4 per house), whereas the number of flowering plants per house increased on the outskirts (range, 3.1-5.4 plants per house). equal proportions of sugar positive females were ... | 1997 | 9439110 |
| evaluation of commercial insect repellents on human skin against aedes aegypti (diptera: culicidae). | an olfactometer was used to evaluate the efficacy of selected commercial insect repellent products against aedes aegypti (l.). a comparison of 12 commercial repellent products was made on human skin. the products tested included 2 natural oil insect repellent formulations (buzz away and green ban) containing plant extracts, 2 proprietary products (skin-so-soft lotion and bath oil), and 8 commercial deet preparations in various concentrations and forms of spray, aerosol, stick, cream, and lotion. ... | 1997 | 9439116 |
| evidence for two distinct members of the amylase gene family in the yellow fever mosquito, aedes aegypti. | genomic dna fragments encoding a salivary gland-specific alpha-amylase gene, amylase i (amy i), and an additional amylase, amylase ii (amyii) of the yellow fever mosquito, aedes aegypti, were isolated and characterized. two independently isolated dna fragments, g34-f and g34-14a, encode polymorphic alleles of amy i. a 3.2 kilobase (kb) ecor i fragment of g34-f, f2, has been sequenced in its entirety and contains 832 base pairs (bp) of the 5'-end, non-coding and putative promoter regions that are ... | 1997 | 9443377 |
| chikungunya in thailand: a re-emerging disease? | the first reported case of chikungunya virus diagnosed by serology in thailand was in 1960 and the last one was in 1991. the disease surveillance system does not specifically include chikungunya cases and the signs and symptoms are similar to these of dengue fever/dengue hemorrhagic fever (df/dhf), rubella, and fever of unknown origin (fuo); thus cases might often be reported under those diagnoses. during the rainy season of 1995 (jun-aug), there were at least 2 reported chikungunya outbreaks wh ... | 1997 | 9444021 |
| first evidence of natural vertical transmission of yellow fever virus in aedes aegypti, its epidemic vector. | entomological investigations were conducted in 1995 in senegal, following a yellow fever (yf) outbreak. a total of 1125 mosquitoes collected in the field, including males, females and 12-48 h old newly emerged adults reared from wild-caught larvae, were tested for yf virus. among the 22 species captured, aedes aegypti was the most common. 'wild' vectors of yf were also captured, including a. furcifer, a. metallicus and a. luteocephalus. in all, 28 yf virus isolations were made: 19 from a. aegypt ... | 1997 | 9463659 |
| evolution of repellent tolerances in representative arthropods. | eight commercial insect repellents were tested against ornithodoros parkeri (acari: argasidae), dermacentor variabilis (acari: ixodidae), aedes aegypti (diptera: culicidae), and xenopsylla cheopis (siphonaptera: pulicidae). patterns of tolerance to the test materials were distinctive for each test species. levels of tolerance were coded as character state 0 (sensitive), 1 (intermediate), or 2 (tolerant) and mapped on a cladogram reflecting the accepted classification of the test species. charact ... | 1997 | 9474558 |
| performance of ulv formulations (pesguard 102/vectobac 12as) against three mosquito species. | adulticidal and larvicidal performances of a water-based pyrethroid microemulsion pesguard ps 102 (ai d-allethrin and d-phenothrin, both at 5.0% w/w) and vectobac 12as, an aqua-suspension bacillus thuringiensis israelensis (b.t.i.) formulation (ai 1,200 itu/mg) were assessed against mosquitoes aedes aegypti, aedes albopictus, and culex quinquefasciatus using a leco ulv fog generator model 1600 and a scorpion 20 ulv airblast sprayer. laboratory-cultured mosquito adults and larvae were used for ef ... | 1997 | 9474567 |
| the mossie-buster: a hose-driven insecticide delivery tool for the control of container-breeding mosquitoes. | a novel insecticide delivery tool, the mossie-buster, was recently developed to control larval populations from urban breeding sites in townsville, australia. this functional and user-friendly control device directly targets the main breeding sites of aedes aegypti (l.) with a focused delivery of insecticides. the mossie-buster comprises a commercially available device and attachments that mix an insecticide solution into the flow of water emitted from a hose. attached to the device is a trigger ... | 1997 | 9474568 |
| a novel insecticidal serotype of clostridium bifermentans. | a novel clostridium bifermentans strain toxic to mosquito larvae on ingestion was isolated from a soil sample collected from secondary forest floor. this strain was designated as serovar paraiba (c. b. paraiba) according to its specific h antigen. clostridium bifermentans paraiba is most toxic to anopheles maculatus theobald larvae (lc50 = 0.038 mg/liter), whereas toxicity to aedes aegypti (linn.) (lc50 = 0.74 mg/liter) and culex quinquefasciatus say (lc50 = 0.11 mg/liter) larvae was 20 and 3 ti ... | 1997 | 9474569 |
| differential accumulation and tissue distribution of mosquito hexamerins during metamorphosis. | the pupal hexamerins were characterized for two mosquitoes representative of the culicine and anopheline families, aedes aegypti and anopheles gambiae. like higher diptera, both mosquito species express two types of hexamerins, hex-1 and hex-2, whose subunits are distinguished by different levels of methionine and aromatic amino acids. in a. aegypti there are two heterohexamers, aahex-1 and aahex-2. in a. gambiae there are two homohexamers, aghex-1.1 and aghex-1.2, and one heterohexamer, aghex-2 ... | 1997 | 9474778 |
| global situation of dengue and dengue haemorrhagic fever, and its emergence in the americas. | about two-thirds of the world's population live in areas infested with dengue vectors, mainly aedes aegypti. all four dengue viruses are circulating, sometimes simultaneously, in most of these areas. it is estimated that up to 80 million persons become infected annually although marked underreporting results in the notification of much smaller figures. currently dengue is endemic in all continents except europe and epidemic dengue haemorrhagic fever (dhf) occurs in asia, the americas and some pa ... | 1997 | 9477544 |
| impact of dengue virus infection on feeding behavior of aedes aegypti. | in addition to heavily infecting the salivary glands of aedes aegypti (l.) mosquitoes, dengue viruses produce a significant infection of the nervous system, involving the brain, johnston's organ, compound eye, and thoracic and abdominal ganglion. to determine if dengue infection affects feeding behavior of ae. aegypti we measured feeding times, counted the number of feeding delays or interruptions, and by in situ immunocytochemistry techniques determined the spatial and temporal distribution of ... | 1997 | 9288801 |
| venereal and vertical transmission of the aedes albopictus parvovirus in aedes aegypti mosquitoes. | following per oral infection of aedes aegypti larvae with aedes albopictus parvovirus (aapv), infected males and females adults were tested for their ability to transmit the virus venereally and vertically, respectively. both types of transmission were observed. a low percentage (2.2%) of aapv-free females were found contaminated by the virus after mating with aapv-infected males. although no significant difference was observed in the fecundity of orally infected and virus-free females, 17.1% of ... | 1997 | 9288802 |
| a fitness advantage for aedes aegypti and the viruses it transmits when females feed only on human blood. | literature on arthropod-borne diseases has traditionally supported the notion that mosquito vectors maintain a feeding duality that includes vertebrate blood meals for egg development and sugar meals from plants for the synthesis of flight and survival energy reserves. aedes aegypti was found to deviate from that feeding pattern by obtaining a reproductive advantage when feeding only on human blood. female mosquitoes fed human blood alone had a greater net replacement rate and intrinsic rate of ... | 1997 | 9288822 |
| factors influencing the duration of blood-feeding by laboratory-reared and wild aedes aegypti (diptera: culicidae) from trinidad, west indies. | the time taken by aedes aegypti to take a bloodmeal was determined under laboratory and field conditions in trinidad. eggs from field-collected females were reared under nutrient-limited (low diet) and nutrient-adequate (high diet) conditions. blood-feeding times indicated that 71% of the females from the low-diet group were fast feeders (< 2 min) compared with 40% of the female from the high-diet group. subsequent tests of the f1 and f2 progeny of the fast-feeding females indicated that the fas ... | 1997 | 9307662 |
| activity of dengue-2 virus and prevalence of aedes aegypti in the chirimiri colliery area, madhya pradesh, india. | repeated outbreaks of a suspected viral fever in chirimiri colliery area, madhya pradesh were reported since 1990. the area consists of an agglomeration of sprawling settlements at varying altitudes of 816 to 890 m and it has partial sylvan cover. during a 1992 outbreak 25 patients' sera were tested, of which 13 showed seropositivity to dengue (den) by mac-elisa test; den-2 was isolated from aedes aegypti collected from two of the eight settlements of the area. the principal vector, ae. aegypti, ... | 1997 | 9322295 |
| detection of dengue virus from field aedes aegypti and aedes albopictus adults and larvae. | mosquito adults and larvae were collected from dengue high risk areas and transported to the laboratory for identification. identified mosquitos were pooled according to the species, date and locality and stored at -70 degrees c. a total of 1,385 pools of aedes albopictus and 267 pools of ae. aegypti were collected from major towns in 12 states in peninsular malaysia. virus isolation was carried out using cell culture (c6/36 clone) of ae. albopictus and detection of dengue virus by the peroxidas ... | 1997 | 9322296 |
| does transovarial transmission of dengue virus occur in malaysian aedes aegypti and aedes albopictus? | 1997 | 9322311 | |
| (2,4-cis and trans)-gigantecinone and 4-deoxygigantecin, bioactive nonadjacent bis-tetrahydrofuran annonaceous acetogenins, from goniothalamus giganteus. | two new acetogenins, (2,4-cis and trans)-gigantecinone (1), isolated as a mixture, and 4-deoxygigantecin (2), a known acetogenin whose absolute stereochemistry has not been determined previously, were isolated using activity-directed fractionation, from the bark of goniothalamus giganteus. a key step in solving their absolute stereochemistries was the preparation of 1,4-diol formaldehyde acetal derivatives (1b and 2a). using the advanced mosher ester method and circular dichroism, the absolute s ... | 1997 | 9322367 |
| dengue infection in paracambi, state of rio de janeiro, 1990-1995. | a seroepidemiological survey was carried out during 1994 in the municipality of paracambi, state of rio de janeiro. haemagglutination inhibition test positivity was detected in 145 out of 370 (39.2%) schoolchildren. the frequency of positive test by sex was 53.8% (78/145) female and 46.2% (67/145) male. distribution by age showed the increasing of antibody positivity in older children. strains of dengue virus type 1 and dengue virus type 2 were isolated before (1990) showing the co-circulation o ... | 1997 | 9322424 |
| immune responsiveness in vector insects. | 1997 | 9326575 | |
| midgut-specific immune molecules are produced by the blood-sucking insect stomoxys calcitrans. | we have cloned and sequenced two defensins, smd1 and smd2, from anterior midgut tissue of the blood-sucking fly stomoxys calcitrans. the dna and n-terminal protein sequences suggest both are produced as prepropeptides. smd1 differs from the classic defensin pattern in having an unusual six-amino acid-long n-terminal sequence. both smd1 and smd2 have lower pi points and charge than insect defensins derived from fat body/hemocytes. northern analysis shows both of these defensin molecules are tissu ... | 1997 | 9326639 |
| cloning, expression and toxicity of a mosquitocidal toxin gene of bacillus thuringiensis subsp. medellin. | bacillus thuringiensis (bt) subsp. medellin (btmed) produces parasporal crystalline inclusions which are toxic to mosquito larvae. it has been shown that the inclusions of this bacterium contain mainly proteins of 94, 68 and 28-30 kda. ecori partially digested total dna of btmed was cloned by using the lambda zap ii cloning kit. recombinant plaques were screened with a mouse polyclonal antibody raised against the 94 kda crystal protein of btmed. one of the positive plaques was selected, and by i ... | 1997 | 9332588 |
| [reflection on 2 current viral diseases: yellow fever and dengue]. | yellow fever and dengue are two current viral diseases induced by flaviviruses and usually transmitted by the same mosquito vector, aedes aegypti. from 1987 to 1991, 18,753 cases of yellow fever, mainly from africa, have been notified to who, leading to 4,522 deaths. on the other hand, who estimates that 2.5 billions individuals living in tropical areas are at risk to contract dengue fevers. in fact, 500,000 patients are hospitalized each year for dengue hemorrhagic fever/dengue shock syndrome a ... | 1997 | 9347008 |
| impact of dengue virus infection and its control. | dengue virus infection has been counted among emerging and re-emerging diseases because of (1) the increasing number of patients, (2) the expansion of epidemic areas, and (3) the appearance of severe clinical manifestation of dengue hemorrhagic fever (dhf)/dengue shock syndrome (dss), which is often fatal if not properly treated. in the meantime, there are no effective dengue control measures: a dengue vaccine is still under development and vector control does not provide a long-lasting effect. ... | 1997 | 9348165 |
| mosquito clathrin heavy chain: analysis of protein structure and developmental expression in the ovary during vitellogenesis. | we have deduced the amino acid sequences of clathrin heavy chain (chc) polypeptides based on cdna and genomic clones from the mosquito, aedes aegypti. two isoforms which differ in the very beginning of the n-terminal domain, ovary-specific aachca and somatic-specific aachcb, were identified, characterized and compared to one another as well as to chc polypeptides from different species. the 1682 amino acid sequence of the aachca isoform predicts a molecular mass (m[r]) of 191,743 daltons and an ... | 1997 | 9359577 |
| improvement of bacillus sphaericus toxicity against dipteran larvae by integration, via homologous recombination, of the cry11a toxin gene from bacillus thuringiensis subsp. israelensis. | integrative plasmids were constructed to enable integration of foreign dna into the chromosome of bacillus sphaericus 2297 by in vivo recombination. integration of the apha3 kanamycin resistance gene by a two-step procedure demonstrated that this strategy was applicable with antibiotic resistance selection. hybridization experiments evidenced two copies of the operon encoding the binary toxin from b. sphaericus in the recipient strain. the bacillus thuringiensis subsp. israelensis cry11aal gene ... | 1997 | 9361428 |
| chymotrypsin inhibitors in mosquitoes: activity profile during development and after blood feeding. | chymotrypsin and trypsin inhibitors persist throughout all developmental instars of aedes aegypti. after a blood meal, inhibitor activity against chymotrypsin was more than double that of sugar-fed females, but only weak activity was detected in midguts where proteinase inhibitors has been thought to regulate proteinases during blood digestion. a fourfold increase in the ratio of abdominal/thoracic inhibitor activity after the blood meal strongly suggested that fat body, or other abdominal tissu ... | 1997 | 9366013 |
| secretory and internalization pathways of mosquito yolk protein precursors. | the vitellogenic female fat body of the mosquito aedes aegypti produces three yolk protein precursors that are deposited in the yolk bodies of developing oocytes: vitellogenin, vitellogenic carboxypeptidase (vcp), and 44-kda protein (44kp). we have used gold immunocytochemistry to investigate the pathways of their secretion in fat body trophocytes and their internalization by oocytes. in fat body trophocytes, all three yolk protein precursors are co-localized in the golgi complex and secretory g ... | 1997 | 9377633 |
| aedes aegypti in córdoba province, argentina. | in 1955, the area infested by aedes aegypti in argentina was estimated as 1,500,000 km2; and in 1963, the species was considered to be eradicated from argentina. in 1995, the argentine ministry of health reported reinfestation by ae. aegypti. during 1994-95, the ministry of health of córdoba province, zoonosis department, established a surveillance system for ae. aegypti in córdoba province, argentina. this report is a summary of results obtained thus far. in total, 74 localities in córdoba prov ... | 1997 | 9383767 |
| molecular cloning and characterization of a novel mosquitocidal protein gene from bacillus thuringiensis subsp. fukuokaensis. | a novel mosquitocidal protein gene, cry20aa, was cloned from bacillus thuringiensis subsp. fukuokaensis (h-3a: 3d: 3e). the gene product, cry20aa, was naturally truncated and had a molecular mass of 86,138 da. the cry20aa protein possessed five conserved sequence blocks, as do most other insecticidal cry toxins. however, an amino acid comparison of cry20aa with other mosquitocidal toxins, including cry4a, cry4b, cry10a, cry11a, and cry11b, demonstrated that cry20aa was quite different from other ... | 1997 | 9406385 |
| mosquito larvicidal activity of transgenic anabaena strain pcc 7120 expressing combinations of genes from bacillus thuringiensis subsp. israelensis. | various combinations of the genes cryiva (cry4a), cryivd (cry11a), and p20 from bacillus thuringiensis subsp. israelensis were introduced into the nitrogen-fixing cyanobacterium anabaena sp. strain pcc 7120 by means of escherichia coli-anabaena shuttle vector prl488p and were expressed under control of two tandem strong promoters, a cyanobacterial promoter (ppsba) and an e. coli t7 promoter (pa1). two of the clones carrying cryiva plus cryivd, one with p20 and one without p20, displayed toxicity ... | 1997 | 9406420 |
| [impregnation of uniforms with permethrin as a mean of protection of working personnel exposed to contact with hematophagous arthropods]. | laboratory bioassays were conducted to evaluate the effectiveness of permethrin-impregnated clothing against biting arthropods. military battle dress uniforms were impregnated at a rate of 0.12-0.15 mg active ingredient (a.i.)/cm2. gas chromatographic analysis of cloth patches treated showed that over 50% of the chemical remained after 6 cycles of washing. this amount of a.i. (0.06-0.07 mg/cm2) was effective in providing protection against aedes aegypti and ixodes ricinus. mosquitoes and ticks w ... | 1997 | 9409928 |
| human dirofilariasis caused by dirofilaria (nochtiella) repens in sri lanka. | human dirofilariasis due to dirofilaria (nochtiella) repens is a common zoonotic infection in sri lanka. todate 70 cases are on record, and they include 3 expatriates from russia, england and korea, who were undoubtedly infected in sri lanka. around 30-60% of dogs are infected with d. repens in various parts of the country and the mosquito vectors are aedes aegypti, armigeres subalbatus, mansonia uniformis and m. annulifera. unlike in other countries of the old world infection is most common in ... | 1997 | 9802095 |
| [dengue in panama, 1993]. | up to 1993, panama was the only country in central america where the autochthonous transmission of dengue virus had been detected without experimenting an explosive epidemic despite being reinfected with the aedes aegypti mosquito since 1985. the characteristics of this first outbreak reported on november 19, 1993, are described in this paper. it is shown that even when there is a program for the surveillance and control of dengue, which considers low levels of aedes aegypti infection and a syst ... | 1997 | 9685969 |
| dengue: a literature review and case study of travelers from the united states, 1986-1994. | dengue fever is an acute, mosquito-transmitted viral disease characterized by fever, headache, arthralgia, myalgia, rash, nausea, and vomiting. infections are caused by any of four virus serotypes (den-1, den-2, den-3, and den-4). the incidence of dengue is increasing in most tropical areas throughout the world (fig. 1). although dengue is not endemic in the continental united states, hawaii, or alaska, more than 500 laboratory-positive cases of introduced dengue were reported from 1977 through ... | 1997 | 9815484 |
| mosquito hexamerins: characterization during larval development. | we report here the first examination of hexamerins expressed during mosquito larval development. haemolymph proteins from fourth-instar larvae of six species representing the two major subfamilies of mosquitoes were characterized by immunoblotting using antisera to calliphorin, the major hexamerin of the blowfly. calliphora vicina, or to lsp1 or lsp2, the two distinct hexamerins of drosophila melanogaster. in each mosquito species the antisera demonstrated the presence of multiple abundant hexam ... | 1997 | 9013251 |
| bioactive compounds from taiwania cryptomerioides. | two new sesquiterpenes, 10 alpha-hydroxyamorphan-4-en-3-one (1) and 4 alpha-methylcadinane-4 alpha-methyl-1 alpha,2 alpha,10 alpha-triol (2), together with four known compounds, sesquiterpenes 10 alpha-hydroxycadinan-4-en-3-one (3) and alpha-cadinol (4), diterpene ferruginol and lignan helioxanthin, were isolated from the whole plant of taiwania cryptomerioides under bioassay-guided fractionations. the structures of 1 and 2 were elucidated mainly by the nmr spectroscopic analyses. bioactivities ... | 1997 | 9014350 |
| identification of a gene for cyt1a-like hemolysin from bacillus thuringiensis subsp. medellin and expression in a crystal-negative b. thuringiensis strain. | a gene designated cyt1ab1, encoding a 27,490-da protein, was isolated from bacillus thuringiensis subsp. medellin (h30 serotype) by using an oligonucleotide probe corresponding to the cyt1aa1 gene. the sequence of the cyt1ab1 protein, as deduced from the sequence of the cyt1ab1 gene, was 86% identical to that of the cyt1aa1 protein and 32% identical to that of the cyt2aa1 protein from b. thuringiensis subsp. kyushuensis. the cyt1ab1 gene was flanked upstream by a p21 gene, in the same orientatio ... | 1997 | 9023925 |
| characterization of aedes communis, aedes aegypti and anopheles stephensi mosquito saliva antigens by immunoblotting. | mosquito bites cause immediate wheals and delayed bite papules in sensitized subjects having saliva-specific ige and igg4 antibodies. at present, mosquito saliva antigens are not well characterized. | 1997 | 9030098 |
| virulence, parasite mode of transmission, and host fluctuating asymmetry. | horizontally transmitted parasites are broadly predicted to be more virulent, or costly to host fitness, than those with vertical transmission. this is mainly because vertical transmission, from host parent to offspring, explicitly links the reproductive interests of both parties. underlying this prediction is a general assumption that parasite transmission success is positively correlated with its virulence. we report results where infection of larval yellow fever mosquitoes aedes aegypti with ... | 1997 | 9061958 |
| the putative cell cycle gene, enhancer of rudimentary, encodes a highly conserved protein found in plants and animals. | the enhancer of rudimentary gene, e(r), in drosophila melanogaster encodes a protein, er, whose function has been implicated in pyrimidine biosynthesis and the cell cycle (wojcik et al. (1994) genetics 138, 1163-1170). in order to identify conserved regions of the protein and potentially important functional domains, the e(r) gene was cloned and sequenced from two other insects (drosophila virilis and aedes aegypti) and three vertebrates (homo sapiens, mus musculus, and brachydanio rerio) and se ... | 1997 | 9074495 |
| pupal survey: an epidemiologically significant surveillance method for aedes aegypti: an example using data from trinidad. | this report documents the results of a country-wide pupal survey of aedes aegypti (l.) conducted in trinidad. the survey was designed to identify the important ae. aegypti-producing containers, importance being a function of a container's abundance and its productivity. results are summarized on a country-wide basis and by county: urban versus rural comparisons are also made. numerically, the most common water-filled containers positive for the larvae or pupae of ae. aegypti (foci) were outdoor ... | 1997 | 9080874 |
| effects of the neem tree compounds azadirachtin, salannin, nimbin, and 6-desacetylnimbin on ecdysone 20-monooxygenase activity. | the effects of azadirachtin, salannin, nimbin, and 6-desacetylnimbin on ecdysone 20-monooxygenase (e-20-m) activity were examined in three insect species. homogenates of wandering stage third instar larvae of drosophila melanogaster, or abdomens from adult female aedes aegypti, or fat body or midgut from fifth instar larvae of manduca sexta were incubated with radiolabeled ecdysone and increasing concentrations (from 1 x 10(-8) to 1 x 10(-3) m) of the four compounds isolated from seed kernels of ... | 1997 | 9131784 |
| analysis of the mosquito lysosomal aspartic protease gene: an insect housekeeping gene with fat body-enhanced expression. | in the fat body of insects with cyclic egg maturation, lysosomes play a critical role in the termination of vitellogenesis by selectively degrading the secretory machinery involved in the massive production of yolk protein precursors. to investigate this fat body-specific lysosomal activity in the mosquito, a cathepsin d-like aspartic protease (lap) was previously purified and its cdna cloned. here we report the isolation of the aalap gene from an aedes aegypti genomic library. the transcribed r ... | 1997 | 9134712 |
| biological effects of canatoxin in different insect models: evidence for a proteolytic activation of the toxin by insect cathepsinlike enzymes. | canatoxin is a toxic protein isolated from the jackbean, canavalia ensiformis. the toxin injected intraperitoneally is lethal for mice and rats; however, it is inactive if given orally. in this study, manduca sexta (l.) (lepidoptera), schistocerca americana (drury) (orthoptera), drosophila melanogaster (l.) (diptera), aedes aegypti (l.) (diptera), rhodnius prolixus (stål) (hemiptera), and callosobruchus maculatus (f.) (coleoptera) were fed on canatoxin-containing diets. no effects were seen in m ... | 1997 | 9145031 |
| factors favoring houseplant container infestation with aedes aegypti larvae in marília, são paulo, brazil. | since reinvasion of são paulo state by the aedes aegypti (l.) mosquito in 1985, flower pots and vases have been important larval habitats despite educational messages focusing on their control. the objectives of this study were to characterize flower pots and vases as larval habitats with respect to the quantities present and infested, the types of plants involved, and the specific locations of the mosquito larvae; to explore local names for houseplants; and to examine factors affecting acceptan ... | 1997 | 9149524 |
| evaluation of caribbean strains of macrocyclops and mesocyclops (cyclopoida:cyclopidae) as biological control tools for the dengue vector aedes aegypti. | fifteen caribbean strains of copepods were assessed for their predation ability against mosquito larvae. macrocyclops albidus from nariva. mesocyclops aspericornis from oropouche, and mesocyclops longisetus from e1 socorro, trinidad, were most effective against aedes aegypti but not against culex quinquefasciatus. mesocyclops longisetus and me. aspericornis prevented any mosquito survival over 25 wk of observation despite weekly challenges with ae. aegypti. the copepods were tolerant to dosages ... | 1997 | 9152870 |
| attractant resting boxes for rapid collection and surveillance of aedes aegypti (l.) inside houses. | three different resting station designs made of cardboard covered with black cloth were evaluated for their attractiveness to aedes aegypti populations resting inside houses in thailand. box designs attracted more females (but not males) than an open-panel design. thirty to 60% of all adult ae. aegypti resting inside houses were collected by aspirating from 2-4 resting boxes placed inside houses. tall boxes (90 cm) did not attract more females or males than short boxes (45 cm). forty-two percent ... | 1997 | 9152871 |
| efficacy of carbon dioxide, 1-octen-3-ol, and lactic acid in modified fay-prince traps as compared to man-landing catch of aedes aegypti. | the attractants 1-octen-3-ol and lactic acid significantly decreased catches of aedes aegypti in townsville, australia, by 50% in a controlled laboratory environment and by 100% in the field when compared to carbon dioxide baited bidirectional fay-prince trap catches. evaluation of an omnidirectional alteration on a bidirectional fay-prince trap revealed no significant improvement in catch size when compared to both the bidirectional trap and man-landing catch (mlc). cumulative evening mlc (1730 ... | 1997 | 9152877 |
| investigation of mosquito-specific larvicidal activity of a soil isolate of bacillus thuringiensis serovar canadensis. | the lc50 value of alkali-solubilized parasporal inclusion proteins of a diptera-specific strain, belonging to bacillus thuringiensis serovar canadensis, was 2.4 microg/ml for larvae of the mosquito, aedes aegypti. a significant loss in larvicidal activity occurred when solubilized inclusion proteins were treated with a. aegypti larval gut extract, silkworm (bombyx mori) larval gut juice, and the proteinase k. approximately 90% of the larvicidal activity was destroyed upon treatment with protease ... | 1997 | 9175558 |
| comparisons of genetic variability and genome structure among mosquito strains selected for refractoriness to a malaria parasite. | restriction fragment length polymorphism (rflp) markers were used to evaluate aedes aegypti genome structure and genetic variability within and between substrains selected for different levels of refractoriness to the malaria parasite, plasmodium gallinaceum. the moyo-r substrain was previously selected for complete refractoriness and the moyo-is substrain for intermediate susceptibility from the moyo-in-dry (moyo) strain by selective inbreeding (f = 0.5). eighteen mapped rflp markers were used ... | 1997 | 9183846 |
| analysis of molecular forms and pharmacological properties of acetylcholinesterase in several mosquito species. | two acetylcholinesterases (ache1 and ache2) have recently been characterized in the common mosquito culex pipiens. this situation appeared to be an exception among insects, where only one acetylcholinesterase gene had previously been repeatedly reported. in the present study, acetylcholinesterase was studied in five mosquito species: aedes aegypti, anopheles gambiae, anopheles stephensi, culiseta longeareolata and culex hortensis, in order to test whether or not two different acetylcholinesteras ... | 1997 | 9185166 |
| failure of aedes aegypti to become infected by feeding on dengue virus-infected immuno-comprised nude mice. | 1997 | 9199718 | |
| three novel families of miniature inverted-repeat transposable elements are associated with genes of the yellow fever mosquito, aedes aegypti. | three novel families of transposable elements, wukong, wujin, and wuneng, are described in the yellow fever mosquito, aedes aegypti. their copy numbers range from 2,100 to 3,000 per haploid genome. there are high degrees of sequence similarity within each family, and many structural but not sequence similarities between families. the common structural characteristics include small size, no coding potential, terminal inverted repeats, potential to form a stable secondary structure, a+t richness, ... | 1997 | 9207116 |
| experimental transmission of nocturnally periodic wuchereria bancrofti to indian leaf monkey (presbytis entellus). | successful experimental transmission of the human lymphatic dwelling nocturnal periodic strain of wuchereria bancrofti has been achieved from man to indian leaf monkey (presbytis entellus) through a susceptible strain of aedes aegypti. the prepatent period varied between 195 and 240 days. microfilaria (mf) levels were in general low and the peak count was attained at 1800 hr. of the adult worms 5-12.5% were recovered from male langur while females revealed poor (2.54%) recovery. this host--paras ... | 1997 | 9207745 |
| characterization of bacillus thuringiensis isolated from infections in burn wounds. | four strains of bacillus thuringiensis were isolated from infections in burn wounds and from water used in the treatment of burn wounds. the strains produced large parasporal inclusion bodies composed of 141, 83, and 81 kda protoxins. the four strains were tested for insecticidal activity against larvae of pieris brassicae and aedes aegypti but showed no activity; vero cell assays for the production of enterotoxins were also negative. attempts to classify the strains according to flagellar h-ser ... | 1997 | 9215586 |
| mixed-species plasmodium infections of anopheles (diptera:culicidae) | mixed-pathogen infections of vectors rarely are considered in the epidemiological literature, although they may occur in nature. a review of published reports shows that many anopheles species are capable of carrying sporozoites of > 1 plasmodium species, of doing so simultaneously in field conditions, and of acquiring and transmitting these in experimental situations. mixed-species infections in mosquito populations occur at frequencies greater than or equal to the product of the constituent sp ... | 1997 | 9220675 |
| infection and transmission of plasmodium gallinaceum (eucoccida: plasmodiidae) in aedes aegypti (diptera: culicidae): effect of preinfection sugar meals and postinfection blood meals. | frequency of sugar feeding and blood feeding can have an impact on the infection by and transmission of malaria parasites. data presented here indicate that frequent blood feeding has a deleterious effect on infection by malaria parasites in aedes aegypti. in addition, mosquitoes that do not blood feed, but instead feed on sugar alone after an infected blood meal, have a higher rate of parasite transmission than mosquitoes fed additional blood meals. | 1997 | 9221737 |
| efficacy of altosid pellets and granules against aedes aegypti in ornamental bromeliads. | the tank bromeliad billbergia pyramidalis was treated with 2 doses (0.5 and 2 g) of altosid granules or pellets for the control of aedes aegypti l. emergence inhibition (ei) for all mosquito pupae (including natural populations) in the center wells and leaf axils was > 90% for at least 6 and 12 months for both doses of granules and pellets, respectively. no significant difference in %ei was found between center wells and leaf axils. | 1997 | 9249661 |
| [a description of aedes aegypti colonization in the region of são josé do rio preto, são paulo]. | the aim of this study is to describe the colonization by the aedes aegypti in the region. a survey carried out in 1985 detected the species in são josé do rio preto. the mosquito has spread and reached the 30 countries of the region till 1988. in the district and rural areas, the first vector focus was found out in 1987 in one of the 29 districts and rural areas, having spread to the others till 1991. the foci have been mainly identified through larval researches in locations with a great concen ... | 1997 | 9265223 |
| the white gene from the yellow fever mosquito, aedes aegypti. | we report the cloning and primary characterization of both cdna and genomic fragments from the white gene of the yellow fever mosquito, aedes aegypti. comparisons of the conceptual translation product with white genes from four other species within the order diptera show that the ae. aegypti gene is most similar to the white gene of the mosquito vector of human malaria, anopheles gambiae (86% identity and 92% similarity). the analysis of the primary sequence of genomic dna at the 5'-end of the c ... | 1997 | 9272447 |
| site-directed mutagenesis of an acetylcholinesterase gene from the yellow fever mosquito aedes aegypti confers insecticide insensitivity. | insecticide resistance is a serious problem facing the effective control of insect vectors of disease. insensitive acetylcholinesterase (ache) confers resistance to organophosphorus (op) and carbamate insecticides and is a widespread resistance mechanism in vector mosquitoes. although the point mutations that underlie ache insensitivity have been described from drosophila, the colorado potato beetle, and house flies, no resistance associated mutations have been documented from mosquitoes to date ... | 1997 | 9371089 |
| selected lines of aedes aegypti with persistently distorted sex ratios. | a breeding scheme to isolate x chromosomes sensitive to drive by the t8 (trinidad) y chromosome of aedes aegypti (the md haplotype) is reported. crosses with an australian strain th.i (thursday island) revealed not only sensitive and resistant x chromosomes but also some with the capacity to drive against the t8 y chromosome. four strains were created in which sex ratio was male-distorted (28-36 per cent female) for 10 generations, with no regression towards sexual parity. the proportion of fema ... | 1997 | 9353868 |
| oviposition responses of aedes aegypti and ae. atropalpus (diptera: culicidae) females to waters from conspecific and heterospecific normal larvae and from larvae infected with plagiorchis elegans (trematoda: plagiorchiidae). | ovipositing aedes aegypti (l.) females were attracted to waters in which uninfected aedes atropalpus (coquillett) larvae had been reared, but were repelled by waters from larvae of either species infected with the entomopathogenic digenean plagiorchis elegans (rudolphi). in contrast, ae. atropalpus females were attracted to or repelled by waters from conspecific uninfected and infected larvae, respectively, but did not respond to corresponding waters from ae. aegypti larvae. the sensitivity of o ... | 1997 | 9379463 |
| isolation and properties of drosophila melanogaster ferritin--molecular cloning of a cdna that encodes one subunit, and localization of the gene on the third chromosome. | ferritin was purified from iron-fed drosophila melanogaster extracts by centrifugation in a gradient of potassium bromide. on polyacrylamide gel electrophoresis, the product showed two protein bands corresponding to the ferritin monomer and dimer. electrophoresis following dissociation with sds and 2-mercaptoethanol revealed three strong bands of approximately 25, 26, and 28 kda. n-terminal amino acid sequences were identical for the 25-kda and 26-kda subunits, but different for the 28-kda subun ... | 1997 | 9266686 |
| effect of ebastine on mosquito bites. | mosquito bites usually cause wealing and delayed bite papules. cetirizine decreases wealing, bite papules and pruritus but the effect of other antihistamines on mosquito bites is unknown. we studied the effect of ebastine in 30 mosquito bite-sensitive adult subjects. ebastine 10 mg or 20 mg and placebo were given for 4 days in a cross-over fashion. aedes aegypti bites were given on forearms. the size of the bite lesions and pruritus (visual analogue score) were measured at 15 min, 2, 6, and 24 h ... | 1997 | 9228228 |
| reduced transfer of male accessory gland proteins and monandry in female aedes aegypti mosquitoes. | we examined whether female aedes aegypti (l.) mosquitoes would remate after they first mated with a male that was reared on a suboptimal larval diet and that as a result, transferred reduced amounts of male accessory gland proteins. accessory gland proteins from males labeled with 3h leucine were not detected in females allowed to male mate with the labeled males after they first mated with either low diet or high diet males. the amount of the male accessory gland protein transferred by smaller, ... | 1997 | 9221745 |
| zebedee: a novel copia-ty1 family of transposable elements in the genome of the medically important mosquito aedes aegypti. | we have utilised pcr to directly identify a novel family of copia-ty1 retrotransposable elements (rtps) in the genome of the mosquito aedes aegypti. two members of the family have been sequenced in their entirety and their structural characteristics determined. zebedeei is 3505 bp long and appears to be flanked by 21bp direct repeat sequences. a single open reading frame (orf) of 972 amino acids has the coding potential for a polyprotein with homology corresponding to the conserved amino acid mo ... | 1997 | 9197409 |
| transposition of the hermes element in embryos of the vector mosquito, aedes aegypti. | using a plasmid-based transpositional recombination assay in vivo, we have demonstrated that hermes, a short inverted repeat type transposable element from musca domestica, can transpose in aedes aegypti embryos. hermes transpositions in ae. aegypti have all the characteristics observed during hermes transposition in its host m. domestica and in related species. these characteristics include an absolute dependence on the expression of the hermes transposase and a preference for the integration s ... | 1997 | 9219363 |
| molecular characterization of a defensin gene from the mosquito, aedes aegypti. | insect immune proteins, defensins, are inducible anti-gram-positive bacterial peptides. we report here the identification of two defensin genes from the mosquito, aedes aegypti, which encode a large 541 bp transcript (aadef ala) and a small 473 bp transcript (aadef asm). the cdna corresponding to aadef ala was cloned, sequenced, and compared with the previously reported aadef asm cdna. the aadef ala gene was isolated through genomic library screening and characterized. it putative regulatory reg ... | 1997 | 9219362 |
| fish landmarks for aedes aegypti chromosomes. | aedes aegypti metaphase chromosome landmarks have been developed so that each chromosome of the haploid genome can be unambiguously identified and oriented by fluorescence in situ hybridization (fish) and digital imaging microscopy. the fish tags were derived from three cosmids that contain repetitive ae. aegypti sequences and their unique fish tagging characteristics are demonstrated. the sequence of the three chromosomal tags revealed that the chromosome 1 tag is an 18s fragment from the ribos ... | 1997 | 9099584 |
| insulin-mediated secretion of ecdysteroids from mosquito ovaries and molecular cloning of the insulin receptor homologue from ovaries of bloodfed aedes aegypti. | the reproductive cycle of female mosquitoes is activated by ingestion of blood from vertebrate hosts. shortly after feeding, neurohormones are released from the brain neurosecretory system and stimulate the ovaries to secrete ecdysteroids, which are necessary for vitellogenesis by the fat body. because bombyxins, which are insulin-like peptides, stimulate ecdysteriodgenesis in silkworm larvae, we tested porcine insulin and found that it activates ecdysteroidogenesis and protein synthesis in ovar ... | 1997 | 9099579 |
| a single cdna encodes all three aedes leucokinins, which stimulate both fluid secretion by the malpighian tubules and hindgut contractions. | a cdna encoding preproleucokinin was isolated from a cdna library of the mosquito aedes aegypti. the deduced amino acid sequence of aedes preproleucokinin contains a putative signal peptide of 18 amino acid residues and a 210-amino acid residue proleucokinin. within the proleucokinin are encoded one copy each of the aedes leucokinins 1, 2, and 3 isolated previously from this species (veenstra, j. a. (1994) biochem. biophys. res. commun. 202, 715-719). all three aedes leucokinins depolarize the t ... | 1997 | 9099680 |
| inheritance of larval resistance to permethrin in aedes aegypti and association with sex ratio distortion and life history variation. | the genetic mechanisms that confer larval permethrin resistance were investigated in two strains of aedes aegypti, vectors of yellow fever and dengue hemorrhagic fever. larval resistance to permethrin in an ae. aegypti field-collected resistant couva (r) strain was associated with the sex-determining locus by analysis of backcrosses to the susceptible rockefeller (s) strain. the median lethal concentrations (lc50s) of these strains were 23.1 (95% confidence interval = 22.0-24.3) and 2.2 (2.0-2.3 ... | 1997 | 9158058 |
| cdna cloning and pattern of expression of an adult, female-specific chymotrypsin from aedes aegypti midgut. | a cdna for a midgut chymotrypsin, induced by a blood meal, has been cloned and sequenced from the mosquito aedes aegypti. the 938 base sequence codes for a 268 amino acid protein, which contains an 18-residue signal peptide and a seven-residue activation peptide. the deduced amino acid sequence contains several features typical of chymotrypsin proteases, including the catalytic triad of serine proteases and the residues that determine the chymotrypsin substrate specificity pocket. the chymotryps ... | 1997 | 9134710 |
| aedes aegypti surveillance and correlation with the occurrence of dengue fever in bahia, brazil. | due to the high frequency of dengue fever cases and the presumed association of such an epidemic with an increase in the population of the mosquito vector, aedes aegypti, we examined the records of the ministry of health in the state of bahia, brazil, regarding the monitoring of domestic mosquito larvae in municipalities throughout the state. the "house index" number for larvae in domestic water reservoirs was determined for each municipality based on annual surveys from 1990 to 1994, and in 199 ... | 1997 | 11107237 |
| effects of two insect growth regulators on the susceptibility of aedes aegypti (diptera:culicidae) to molinema dessetae (nematoda:filarioidea). | the effects of 2 growth regulators, diflubenzuron (dfb) and oms 2017, on the ability of females aedes aegypti to become infected with molinema dessetae was studied under laboratory conditions. oms 2017 and dfb had no effect on either the amount of blood ingested or the microfilarial load. the infective potential of females that survived dfb treatment was significantly greater than untreated females, but there was no difference between oms 2017-treated and control females. the percentage of infec ... | 1997 | 9152874 |
| methoprene affects the rotation of the male terminalia of aedes aegypti mosquitoes. | the rotation of the male terminalia of aedes aegypti mosquitoes was investigated with chemical surgical, and mechanical methods. neither decapitation nor ventral nerve cord transection affected the postemergence rotation, but methoprene administered to 4th-instar larvae and early instar pupae slowed rotation significantly and, in the case of pupal treatment, also interfered with successful insemination. methoprene appeared to interfere with the developmental maturation of the cross muscles locat ... | 1997 | 9152867 |
| biochemical, molecular, and phylogenetic analysis of pyruvate carboxylase in the yellow fever mosquito, aedes aegypti. | pyruvate carboxylase (pc, pyruvate: carbon dioxide ligase [adp-forming], ec 6.4.1.1) was purified from the yellow fever mosquito, aedes aegypti. the purified pc showed two polypeptides of similar m(r) (133 and 128 k). the n-terminal sequences of both polypeptides were shown to be very similar, if not identical. a polyclonal antiserum against the 133 kda polypeptide cross-reacted strongly with the 128 kda polypeptide. pc was found in all tissues examined. using a semi-quantitative western blot as ... | 1997 | 9066123 |
| juvenile hormone controls early trypsin gene transcription in the midgut of aedes aegypti. | early trypsin is a female-specific protease present in the aedes aegypti midgut during the first few hours after ingestion of a blood meal. the enzymatic activity of early trypsin plays an essential role in the transcriptional activation of the late trypsin gene, which encodes the major midgut endoprotease involved in blood meal protein digestion. transcription of the early trypsin gene is part of the normal post-emergence maturation of the midgut in the adult female. abdominal ligation within 1 ... | 1997 | 9013256 |
| identification of three allatostatins and their cdna from the mosquito aedes aegypti. | three allatostatins have been isolated from the mosquito aedes aegypti. these peptides have the following structures: ser-pro-lys-tyr-asn-phc-gly-leu-amide, leu-pro-his-tyr-asn-phe-gly-leu-amide, and arg-val-tyr-asp-phe-gly-leu-amide. a cdna encoding these peptides was isolated from an abdominal ganglia cdna library and sequenced. it was found to encode two additional allatostatins: ala-ser-ala-tyr-arg-tyr-his-phe-gly-leu-amide and leu-pro-asn-arg-tyr-asn-phe-gly-leu-amide. northern analysis of ... | 1997 | 9357049 |
| structure of the gypsy moth vitellogenin gene. | genomic clones containing the vitellogenin (vg) gene from the gypsy moth were isolated from two genomic libraries and characterized. the nucleotide sequence of a 16,132 bp region of the gypsy moth genome was determined which included a 3,666 bp region upstream from the transcription initiation site and 499 bp region downstream from the transcribed region. primer extension analysis was performed to identify the transcription initiation site. gene sequence confirmed the sequence of vgmrna recently ... | 1997 | 9327584 |
| central projections of the maxillary and antennal nerves in the mosquito aedes aegypti | in the mosquito aedes aegypti, co2-sensitive receptor neurones are located together with two other types of chemoreceptor neurones in club-shaped sensilla basiconica on the most distal segment of the maxillary palps. in order to identify the central target neuropiles of these neurones and to determine whether antennal receptor neurones project into the same area, the palpal and antennal nerves were labelled by anterograde staining with horseradish peroxidase and by experimentally induced degener ... | 1997 | 9319784 |
| the feasibility of eradicating aedes aegypti in the americas. | 1997 | 9128110 |