Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| the use of a chemically defined artificial diet as a tool to study aedes aegypti physiology. | aedes aegypti mosquitoes obtain from vertebrate blood nutrients that are essential to oogenesis, such as proteins and lipids. as with all insects, mosquitoes do not synthesize cholesterol but take it from the diet. here, we used a chemically defined artificial diet, hereafter referred to as substitute blood meal (sbm), that was supplemented with cholesterol to test the nutritional role of cholesterol. sbm-fed and blood-fed mosquitoes were compared regarding several aspects of the insect physiolo ... | 2015 | 26578294 |
| screening for mosquito larvicidal activity of thai mushroom extracts with special reference to steccherinum sp against aedes aegypti (l.) (diptera: culicidae). | for over 50 years, biological control of mosquito larvae has depended mainly on plant extracts, fish, bacteria, protozoa, filamentous fungi, viruses or nematodes. in this study, we screened 143 mushroom samples from 44 confirmed species in thailand for their mosquito larvicidal activity. one g% (w/v) aqueous extracts of dried powdered mushroom samples were tested against 3rd stage aedes aegypti larvae. four mushroom species, namely, thaeogyroporus porentosus, xylaria nigripes, chlorophyllum sp a ... | 2015 | 26867377 |
| absence of aedes aegypti (l.) on an ecological island: competitive exclusion? | ovitrap surveillance was conducted in 2012 and 2006 in malay and aboriginal villages on carey island. in each village, standard ovitraps were placed indoors and outdoors at randomly selected houses/locations. all l3 larvae recovered were identified up to species level. results demonstrated that only larvae of aedes albopictus were found in all the positive ovitraps placed indoors and outdoors. in 2012, a high ovitrap index (oi) of 66.7% indoor and 84.0% outdoor in the malay village; and 62.5% in ... | 2015 | 26863856 |
| forced egg retention induced by diethyl-phenylacetamide diminishes the fecundity and longevity of dengue vectors. | oviposition is an important event in the life cycle of mosquito which is a decisive factor for the fate of its progeny. if oviposition is prevented, the life cycle of mosquito is disrupted and the population growth can be reduced. diethyl-phenylacetamide (depa) is an effective topical repellent against various haematophagous insects. the study reports the egg retention effect and subsequent lethal effect of depa on the gravid females of aedes aegypti and ae. albopictus mosquitoes. | 2015 | 26714511 |
| synthesis of silver nanoparticles using catharanthus roseus root extract and its larvicidal effects. | phytosynthesis of silver nanoparticles has attracted considerable attention due to their biocompatibility, low toxicity, cost-effectiveness and being a novel method has an eco-friendly approach. biological activity of root extracts as well as synthesized silver nanoparticles of catharanthus roseus were evaluated against larvae of aedes aegyptiand culex quinquefasciatus. the structure and proportion of the synthesized nanoparticles was defined by exploitation ultraviolet spectrophotometry, x-ray ... | 2015 | 26688962 |
| effect of isodillapiole on the expression of the insecticide resistance genes gste7 and cyp6n12 in aedes aegypti from central amazonia. | the yellow fever mosquito aedes (stegomyia) aegypti is the main vector of dengue arbovirus and other arboviruses. dengue prevention measures for the control of a. aegypti involve mainly the use of synthetic insecticides. the constant use of insecticides has caused resistance in this mosquito. alternative studies on plant extracts and their products have been conducted with the aim of controlling the spread of the mosquito. dillapiole is a compound found in essential oils of the plant piper adunc ... | 2015 | 26681019 |
| aedes aegypti eggs oviposited on water surface collected from field ovitraps in nova iguaçu city, brazil. | aedes aegypti eggs can be collected from the water surface. | 2015 | 26676505 |
| suitability of mixing fluorescent dye in adulticides and its impact on droplet characteristics and pesticide efficacy. | fluorescent dyes are commonly used to help visualize insecticidal droplets or to trace movement of insecticides; however, the effect these dyes have on the insecticide's efficacy and droplet characteristics is unknown. this study evaluated the effects of mixing uvitex ob fluorescent dye with 5 adulticides on their efficacy in a wind tunnel. efficacy was determined via droplet size characteristics, spray flux (active ingredient [ai] deposition), and female adult aedes aegypti mortality. fyfanon® ... | 2015 | 26675457 |
| interspecific cross-mating between aedes aegypti and aedes albopictus laboratory strains: implication of population density on mating behaviors. | mating behavior between aedes aegypti and ae. albopictus, established colony strains were examined under laboratory conditions (30-cm(3) screened cages) for 5 consecutive days. the effect of selected male densities (30, 20, 10) and female density (20) on the number of swarming, mating pairs, eggs produced, and inseminated females were evaluated. male densities significantly increased swarming behavior, mating pairs, and egg production of heterospecific females, but female insemination was reduce ... | 2015 | 26675452 |
| new records of aedes aegypti at the southern limit of its distribution in buenos aires province, argentina. | 2015 | 26611978 | |
| the effects of plant essential oils on escape response and mortality rate of aedes aegypti and anopheles minimus. | the high throughput screening system (hitss) has been applied in insecticide behavioral response studies with various mosquito species. in general, chemical or natural compounds can produce a range of insect responses: contact irritancy, spatial repellency, knock-down, and toxicity. this study characterized these actions in essential oils derived from citronella, hairy basil, catnip, and vetiver in comparison to deet and picaridin against aedes aegypti and anopheles minimus mosquito populations. ... | 2015 | 26611967 |
| intraspecific variation in desiccation survival time of aedes aegypti (l.) mosquito eggs of australian origin. | aedes aegypti (l.) mosquitoes preferentially oviposit in natural and artificial receptacles where their eggs are able to withstand drying as water levels fluctuate. desiccation-resistant eggs also increase the potential for establishment in non-native habitats while providing logistical impediments to control programs. viability and mean survival times of eggs stored under three dryness conditions for up to 367 days were investigated among three field-derived colonies of australian ae. aegypti t ... | 2015 | 26611964 |
| larvicidal activity, inhibition effect on development, histopathological alteration and morphological aberration induced by seaweed extracts in aedes aegypti (diptera: culicidae). | to investigate the larvicidal activity, inhibition effect on development, histopathological alteration and morphological aberration induced by the extracts derived from seaweeds bryopsis pennata (b. pennata), sargassum binderi (s. binderi) and padina australis in aedes aegypti (ae. aegypti) larvae and to characterize the phytochemical components of the three seaweeds. | 2015 | 26706671 |
| trace samples of human blood in mosquitoes as a forensic investigation tool. | investigations of any type of crime invariably starts at the crime scene by collecting evidence. thus, the purpose of this research was to collect and analyze an entomological trace from an environment that is similar to those of indoor crime scenes. hematophagous mosquitoes were collected from two residential units; saliva of volunteers that were residents in the units was also collected for genetic analysis as reference samples. we examined the allele frequencies of 15 short tandem repeat loci ... | 2015 | 26600546 |
| [zika virus: another emerging arbovirus in venezuela?]. | current health conditions of venezuela, together with the tropical proliferation of different types of aedes (aegypti and albopictus), suggest the arrival to this country of zika virus (zikv). the pan american health organization urges latin american countries to take measures to prevent the zikv entry and advises that these measures must be designed to detect the introduction of zikv in an area, track its spread and actively monitor the disease. in venezuela and other latin-american countries, ... | 2015 | 26710538 |
| comparative efficacy of existing surveillance tools for aedes aegypti in western kenya. | all traditional surveillance techniques for aedes aegypti have been developed for the cosmopolitan domestic subspecies ae. aegypti aegypti, and not the sylvatic subspecies, ae. aegypti formosus. the predominant form in western kenya is ae. aegypti formosus that is rarely associated with human habitations but is linked to transmission of sylvatic dengue virus strains. we compared five surveillance methods for their effectiveness in sampling ae. aegypti formosus with the goal of determining a sust ... | 2015 | 26611965 |
| natural vertical transmission of dengue viruses in aedes aegypti in selected sites in cebu city, philippines. | we attempted to determine the vertical transmission of dengue virus (denv) in aedes aegypti in selected sites in cebu city, philippines. mosquito sub-adults were collected monthly from households and the field during the wet-dry-wet season from november, 2011 to july, 2012 and were laboratory-reared to adults. viral rna extracts in mosquitoes were assayed by hemi-nested rt-pcr. results showed that 62 (36.26%; n=679) out of 171 mosquito pools (n=2,871) were denv+. the minimum infection rate (mir) ... | 2015 | 26611963 |
| innate antiviral immunity against dengue virus. | dengue virus (denv), the most prevalent mosquito-borne viral diseases in humans worldwide, causes dengue fever, a mild form of the disease, as well as dengue hemorrhagic fever/dengue shock syndrome, a more severe form which can be life-threatening. the four serotypes of denv (denv1-4) are positive-sense, single stranded rna virus belonging to the flaviviridae family and are transmitted by aedes aegypti and aedes albopictus mosquitoes. together, they are estimated to cause almost 100 million symp ... | 2015 | 26559229 |
| [estimation of aedes aegypti (l.) (diptera: culicidae) productivity in households and public spaces in a dengue endemic city in colombia]. | aedes aegypti is a vector for the dengue virus in colombia. its productivity can be estimated using pupal counts. | 2015 | 26535548 |
| essential oils from zanthoxylum fagara wild lime, ruta chalepensis l. and thymus vulgaris l.: composition and activity against aedes aegypti larvae. | the dengue virus is transmitted by aedes aegypti. several plants are used to control this mosquito. in the present study the chemical composition of the essential oils of ruta chalepensis, zanthoxylum fagara and thymus vulgaris were analyzed, and their activities against larvae of two a. aegypti populations were evaluated. the major compounds found in t. vulgaris were thymol and -cymene at 39.8% and 30.5%, respectively, with the major components being oxygenated monoterpenes and monoterpene hyd ... | 2015 | 26525020 |
| dengue fever and dengue hemorrhagic fever in adults. | dengue fever and dengue hemorrhagic fever are re-emerging diseases that are endemic in the tropics. the global prevalence of dengue cases has increased in south-east asia, africa, the western pacific, and the americas. the increasingly widespread distribution and the rising incidence of dengue virus infections are related to increased distribution of aedes aegypti, an increasingly urban population, and increasing air travel. several southeast asian countries show that the age of the reported den ... | 2015 | 26506734 |
| a role for vector control in dengue vaccine programs. | development and deployment of a successful dengue virus (denv) vaccine has confounded research and pharmaceutical entities owing to the complex nature of denv immunity and concerns over exacerbating the risk of denv hemorrhagic fever (dhf) as a consequence of vaccination. thus, consensus is growing that a combination of mitigation strategies will be needed for denv to be successfully controlled, likely involving some form of vector control to enhance a vaccine program. we present here a determin ... | 2015 | 26478199 |
| erratum to: nanoparticles in the fight against mosquito-borne diseases: bioactivity of bruguiera cylindrica-synthesized nanoparticles against dengue virus den-2 (in vitro) and its mosquito vector aedes aegypti (diptera: culicidae). | 2015 | 26412056 | |
| sterol carrier protein 2, a critical host factor for dengue virus infection, alters the cholesterol distribution in mosquito aag2 cells. | host factors that enable dengue virus (denv) to propagate in the mosquito host cells are unclear. it is known that cellular cholesterol plays an important role in the life cycle of denv in human host cells but unknown if the lipid requirements differ for mosquito versus mammalian. in mosquito aedes aegypti, sterol carrier protein 2 (scp-2) is critical for cellular cholesterol homeostasis. in this study, we identified scp-2 as a critical host factor for denv production in mosquito aag2 cells. tre ... | 2015 | 26336241 |
| comparison of the insecticidal characteristics of commercially available plant essential oils against aedes aegypti and anopheles gambiae (diptera: culicidae). | aedes aegypti and anopheles gambiae are two mosquito species that represent significant threats to global public health as vectors of dengue virus and malaria parasites, respectively. although mosquito populations have been effectively controlled through the use of synthetic insecticides, the emergence of widespread insecticide-resistance in wild mosquito populations is a strong motivation to explore new insecticidal chemistries. for these studies, ae. aegypti and an. gambiae were treated with c ... | 2015 | 26336230 |
| detection of dengue virus in mosquito extracts and human clinical samples using a field expedient molecular platform. | dengue fever occurs in localized outbreaks and can significantly erode troop strength and mission readiness. timely identification of dengue virus (denv) provides for rapid and appropriate patient management decisions, such as medical evacuation and supportive therapies, as well as help to promote force health protection through vector control and personal protective measures. the "ruggedized" advanced pathogen identification device is a field-friendly pcr (polymerase chain reaction) platform th ... | 2015 | 26327544 |
| nanoparticles in the fight against mosquito-borne diseases: bioactivity of bruguiera cylindrica-synthesized nanoparticles against dengue virus den-2 (in vitro) and its mosquito vector aedes aegypti (diptera: culicidae). | mosquitoes are blood-feeding insects serving as the most important vectors for spreading human pathogens and parasites. dengue is a viral disease mainly vectored through the bite of aedes mosquitoes. its transmission has recently increased in urban and semi-urban areas of tropical and subtropical regions worldwide, becoming a major international public health concern. there is no specific treatment for dengue. its prevention and control solely depend on effective vector control measures. mangrov ... | 2015 | 26290219 |
| role of aedes aegypti and aedes albopictus during the 2011 dengue fever epidemics in hanoi, vietnam. | to record the human cases of dengue fever (df) and investigate the aedes mosquito species circulating during the hanoi 2011 df epidemics. | 2015 | 26276285 |
| different serotypes of dengue virus (denv) imported by polish travellers from dengue endemic areas to poland. | dengue viruses are the most widespread arboviruses (transmitted mainly by aedes aegypti and ae. albopictus mosquitoes), which have shown an unexpected geographic expansion. there are four dengue virus serotypes: denv-1, denv-2, denv-3, and denv-4. subsequent infections increase the risk of developing severe dengue fever. | 2015 | 26119675 |
| detection of dengue virus in individual aedes aegypti mosquitoes in delhi, india. | delhi, the capital city of india, has so far witnessed several outbreaks of dengue fever since 1967 (last one reported in 2013). improved virological and entomological surveillance are the only tools that can help in prevention of dengue as well as in the development of dengue control programmes. the aim of the study was to conduct a prospective field study to detect dengue virus in adult aedes aegypti mosquitoes collected from various localities represented by different socioeconomic groups in ... | 2015 | 26119544 |
| [vertical transmission of dengue virus in aedes aegypti, peru]. | 2015 | 26102125 | |
| [dengue in peru: a quarter century after its reemergence]. | a health problem each time more frequent and dispersed in tropical and subtropical areas of the world, including peru where it entered in 1990, is dengue. it is produced by the dengue virus with four serotypes and transmitted by aedes aegypti, a vector that coexists with humans and whose presence is favored by deficient sanitary, social and economic conditions. manifestations of severe forms of the disease such as shock and bleeding, are related to the frequent co-circulation of the four serotyp ... | 2015 | 26102118 |
| green-synthesized silver nanoparticles as a novel control tool against dengue virus (den-2) and its primary vector aedes aegypti. | dengue is an arthropod-borne viral infection mainly vectored through the bite of aedes mosquitoes. recently, its transmission has strongly increased in urban and semi-urban areas of tropical and sub-tropical regions worldwide, becoming a major international public health concern. there is no specific treatment for dengue. its prevention and control solely depends on effective vector control measures. in this study, we proposed the green-synthesis of silver nanoparticles (agnp) as a novel and eff ... | 2015 | 26063530 |
| development and validation of an arthropod maceration protocol for zoonotic pathogen detection in mosquitoes and fleas. | arthropod-borne diseases remain a pressing international public health concern. while progress has been made in the rapid detection of arthropod-borne pathogens via quantitative real-time (qpcr), or even hand-held detection devices, a simple and robust maceration and nucleic acid extraction method is necessary to implement biosurveillance capabilities. in this study, a comparison of maceration techniques using five types of beads followed by nucleic acid extraction and detection were tested usin ... | 2015 | 26047188 |
| dengue virus detection in aedes aegypti larvae from southeastern brazil. | the transmission of dengue, the most important arthropod-borne viral disease in brazil, has been intensified over the past decades, along with the accompanying expansion and adaptation of its aedes vectors. in the present study, we mapped dengue vectors in ouro preto and ouro branco, minas gerais, by installing ovitraps in 32 public schools. the traps were examined monthly between september, 2011 through july, 2012 and november, 2012 to april, 2013. the larvae were reared until the fourth stadiu ... | 2015 | 26047186 |
| sero-prevalence of dengue infections in the kassala state in the eastern part of the sudan in 2011. | dengue fever is a vector-borne disease that is transmitted to humans by infected aedes aegypti mosquitoes. the eastern part of the sudan is one of the regions that is affected by dengue virus circulation. in this study, we estimated the prevalence of dengue infections in the kassala state in the eastern part of the sudan. the study objective was to estimate the sero-prevalence of dengue igg/igm antibodies in the kassala locality in 2011. this was a cross sectional community-based study that util ... | 2015 | 25975993 |
| natural transovarial transmission of dengue virus 4 in aedes aegypti from cuiabá, state of mato grosso, brazil. | dengue is the most prevalent arboviral disease in tropical areas. in mato grosso, outbreaks are reported every year, but studies on dengue in this state are scarce. | 2015 | 25860459 |
| susceptibility of indigenous and transplanted mosquito spp. to dengue virus in japan. | dengue fever, an acute, mosquito-borne, febrile illness caused by flavivirus spp., is a problem in africa, south and southeast asia, latin america, and the caribbean. a dengue outbreak occurred after nearly 70 years of absence or no detection, and then 158 autochthonous cases occurred in japan from august to october 15, 2014. the most competent mosquito vectors for dengue virus transmission were aedes aegypti and a. albopictus. since a. albopictus is widely distributed across japan and a. aegypt ... | 2015 | 25766611 |
| assessment of the relationship between entomologic indicators of aedes aegypti and the epidemic occurrence of dengue virus 3 in a susceptible population, são josé do rio preto, são paulo, brazil. | the aims of this study were to describe the occurrence of dengue in space and time and to assess the relationships between dengue incidence and entomologic indicators. we selected the dengue autochthonous cases that occurred between september 2005 and august 2007 in são josé do rio preto to calculate incidence rates by month, year and census tracts. the monthly incidence rates of the city were compared to the monthly breteau indices (bi) of the são josé do rio region. between december 2006 and f ... | 2015 | 25484110 |
| dengue virus 4 (denv-4) re-emerges after 30 years in brazil: cocirculation of denv-2, denv-3, and denv-4 in bahia. | dengue fever (df) is a mosquito-borne viral disease of great concern in tropical and subtropical regions of the world. one important cause of the increase in df is rapid development and urbanization has led to proliferation of the aedes aegypti mosquito, the vector responsible for transmission of the illness. surveillance of dengue virus (denv) infection in brazil shows the predominance of denv-1, denv-2, and denv-3 until 2010. this study reports the reappearance of denv-4 in brazil for the firs ... | 2015 | 25420650 |
| dengue fever in europe: could there be an epidemic in the future? | dengue virus (denv) is the arbovirus with the widest impact on human health. although its dispersal is partially conditioned by environmental constraints that limit the distribution of its main vector (aedes aegypti), denv has been spreading geographically in recent times, but mostly afflicting tropical and subtropical regions. with no prophylactic vaccine or specific therapeutics available, vector control remains the best alternative to restrain its circulation. moreover, the establishment of t ... | 2015 | 25399556 |
| protein-protein interactions between a. aegypti midgut and dengue virus 2: two-hybrid screens using the midgut cdna library. | dengue virus (denv) is principally transmitted by the aedes aegypti mosquito. to date, mosquito population control remains the key strategy for reducing the continuing spread of denv. the focus on the development of new vector control strategies through an understanding of the mosquito-virus relationship is essential, especially targeting the midgut, which is the first mosquito organ exposed to denv infection. | 2015 | 26719940 |
| first evidence of dengue virus infection in wild caught mosquitoes during an outbreak in assam, northeast india. | dengue is one of the major public health problems worldwide, transmitted mainly by aedes aegypti and ae. albopictus mosquitoes. rapid urbanisation and industrialisation have led to an increase in vector population in northeastern states of india. in 2013, guwahati, the capital city of assam, india experienced an outbreak of dengue. this study was undertaken with an objective to determine infection rates of dengue viruses (denv) in both the established vectors present in this region. | 2015 | 26714508 |
| the consequences of co-infections for parasite transmission in the mosquito aedes aegypti. | co-infections may modify parasite transmission opportunities directly as a consequence of interactions in the within-host environment, but also indirectly through changes in host life history. furthermore, host and parasite traits are sensitive to the abiotic environment with variable consequences for parasite transmission in co-infections. we investigate how co-infection of the mosquito aedes aegypti with two microsporidian parasites (vavraia culicis and edhazardia aedis) at two levels of larva ... | 2015 | 25311642 |
| interaction of lysinibacillus sphaericus binary toxin with mosquito larval gut cells: binding and internalization. | the binary toxin produced by lysinibacillus sphaericus is composed of bina and binb subunits. together, but not separately, the two subunits are highly toxic to culex quinquefasciatus larvae, but show no toxicity to aedes aegypti. the molecular mechanism underlying intoxication has not been clearly elucidated. the present study compares the binding and the internalization of binary toxin into the midgut epithelial cells of susceptible c. quinquefasciatus mosquito larvae with those of bin-refract ... | 2015 | 26408968 |
| functional implications of the peptidoglycan recognition proteins in the immunity of the yellow fever mosquito, aedes aegypti. | peptidoglycan recognition proteins (pgrps) play essential roles in the immune systems of insects and higher animals against certain pathogens, including bacteria. in insects, most studies on the functions of pgrps have been performed in drosophila, with only limited studies in mosquitoes, which are important disease vectors. in the present study, we analysed the pgrp sequences of the yellow fever mosquito, aedes aegypti, acquired from two genome databases, and identified a total of seven pgrp ge ... | 2015 | 25588548 |
| molecular characterization of an apolipophorin-iii gene from the chinese oak silkworm, antheraea pernyi (lepidoptera: saturniidae). | apolipophorin-iii (apolp-iii) acts in lipid transport, lipoprotein metabolism, and innate immunity in insects. in this study, an apolp-iii gene of antheraea pernyi pupae (ap-apolp-iii) was isolated and characterized. the full-length cdna of ap-apolp-iii is 687 bp, including a 5'-untranslated region (utr) of 40 bp, 3'-utr of 86 bp and an open reading frame of 561 bp encoding a polypeptide of 186 amino acids that contains an apolipophorin-iii precursor domain (pf07464). the deduced ap-apolp-iii pr ... | 2015 | 25348706 |
| vector competence of aedes aegypti mosquitoes for filarial nematodes is affected by age and nutrient limitation. | mosquitoes are one of the most important vectors of human disease. the ability of mosquitoes to transmit disease is dependent on the age structure of the population, as mosquitoes must survive long enough for the parasites to complete their development and infect another human. age could have additional effects due to mortality rates and vector competence changing as mosquitoes senesce, but these are comparatively poorly understood. we have investigated these factors using the mosquito aedes aeg ... | 2015 | 25446985 |
| mosquito larvicidal and pupaecidal potential of prodigiosin from serratia marcescens and understanding its mechanism of action. | mosquitoes spread lethal diseases like malaria and dengue fever to humans. considering mosquito vector control as one of the best alternatives to reduce new infections, here we have analyzed the effect of purified pigment prodigiosin extracted from serratia marcescens (nmcc 75) against larval and pupal stages of aedes aegypti and anopheles stephensi mosquitoes. mosquito larvicidal activities of purified prodigiosin revealed lc50 values of 14 ± 1.2, 15.6 ± 1.48, 18 ± 1.3, 21 ± 0.87 µg/ml against ... | 2015 | 26267052 |
| chemical composition and biological activity of essential oils of dracocephalum heterophyllum and hyssopus officinalis from western himalaya. | the essential oils of two representatives of the lamiaceae, dracocephalum heterophyllum benth. and hyssopus officinalis l., are described for their antifungal, antibacterial, larvicidal and inect biting deterrent activities. additionally, the chemical compositions of the essential oils, analyzed by simultaneous gc-ms and gc-fid, and odor descriptions are given. the main components of h. officinalis oil were pinocarvone, cis-pinocamphone, and β-pinene. citronellol was found as the main compound o ... | 2015 | 25920235 |
| behavioral response of aedes aegypti (diptera: culicidae) larvae to synthetic and natural attractants and repellents. | aedes aegypti (l.) (diptera: culicidae) is the key vector of three important arboviral diseases: dengue, yellow fever, and chikungunya. immature stages of this species inhabit human-made containers placed in residential landscapes. in this study, we evaluated a few compounds in a sensitive behavioral assay with ae. aegypti larvae. the orientation of larvae to different compounds was surveyed using a performance index (pi). the pi represents the response to each odorant, where a value of +1 is in ... | 2015 | 26352935 |
| efficient transposition of the youngest miniature inverted repeat transposable element family of yellow fever mosquito in yeast. | miniature inverted repeat transposable elements (mites) are often the most numerous dna transposons in plant and animal genomes. the dramatic amplification of mite families during evolution is puzzling, because the transposase sources for the vast majority of mite families are unknown. the yellow fever mosquito genome contains > 220-mb mite sequences; however, transposition activity has not been demonstrated for any of the mite families. the gnome elements are the youngest mite family in this ge ... | 2015 | 25754725 |
| di-rhamnolipid is a mosquito pupicidal metabolite from pseudomonas fluorescens (vcrc b426). | pseudomonas fluorescens migula (vcrc b426) produces a secondary metabolite, which was found to be active against pupae of vector mosquitoes namely culex quinquefasciatus, anopheles stephensi and aedes aegypti. the mosquito pupicidal metabolite from p. fluoescens was mass produced and separated by ethyl acetate extraction and purified further by silica gel column chromatography, fplc, hplc and tlc. the purified metabolite was characterized by nmr, ft-ir, lc-ms and maldi-tof. the ft-ir, (1)h and ( ... | 2015 | 25912083 |
| inter-epidemic abundance and distribution of potential mosquito vectors for rift valley fever virus in ngorongoro district, tanzania. | background rift valley fever (rvf) is a mosquito-borne viral zoonosis that primarily affects ruminants but also has the capacity to infect humans. objective to determine the abundance and distribution of mosquito vectors in relation to their potential role in the virus transmission and maintenance in disease epidemic areas of ngorongoro district in northern tanzania. methods a cross-sectional entomological investigation was carried out before the suspected rvf outbreak in october 2012. mosquitoe ... | 2015 | 28156815 |
| effects of artesunate treatment on plasmodium gallinaceum transmission in the vectors aedes aegypti and culex quinquefasciatus. | in the absence of vaccines, chemotherapy is an effective and economical way for controlling malaria. development of anti-malarial drugs that target pathogenic blood stage parasites and gametocytes is preferable for the treatment as it can alleviate the host's morbidity and mortality and block transmission of the plasmodium parasite. recently, our laboratory has developed an in vivo transmission blocking assay that involves administration of 7 consecutive daily doses of a test compound into domes ... | 2015 | 25466617 |
| effects of proteinase inhibitor from adenanthera pavonina seeds on short- and long term larval development of aedes aegypti. | currently, one of the major global public health concerns is related to the transmission of dengue/yellow fever virus by the vector aedes aegypti. the most abundant digestive enzymes in ae. aegypti midgut larvae are trypsin and chymotrypsin. since protease inhibitors have the capacity to bind to and inhibit the action of insect digestive proteinases, we investigated the short- and long-term effects of adenanthera pavonina seed proteinase inhibitor (apti) on ae. aegypti larvae, as well as a possi ... | 2015 | 25796215 |
| [management of the yellow fever epidemic in 2010 in séguéla (côte d'ivoire): value of multidisciplinary investigation]. | in august 2010, five positive cases of yellow fever were reported in the region of séguéla, in the northwest of côte d'ivoire, affected by an armed conflict since 2002. in order to evaluate the extent of yellow fever virus circulation and the risk for local people, a multidisciplinary investigation was carried out by the côte-d'ivoire ministry of health and public hygiene. | 2015 | 25629680 |
| virulency of novel nanolarvicide from trichoderma atroviride against aedes aegypti (linn.): a clsm analysis. | aedes aegypti is the vector for transmitting dengue, chikungunya, and yellow fever. these diseases' transmission has increased predominantly in urban and semi-urban areas as a major public health concern. in present investigation, trichoderma atroviride culture filtrates were used for the synthesis of silver nanoparticle. moreover, t. atroviride is a free-living and rapidly growing fungi common in soil and root ecosystem. this fungi is an exceptionally good model for biocontrol and more signific ... | 2015 | 25907629 |
| efficacy of tolypocladium cylindrosporum against aedes aegypti eggs, larvae and adults. | the aim of this study was to test the activity of seven tolypocladium cylindrosporum isolates against different developmental stages of aedes aegypti. | 2015 | 26332164 |
| [detection of flavivirus in mosquitoes (diptera: culicidae) from easter island-chile]. | flaviviruses are arthropod-borne viruses, mainly by mosquitoes of the genera aedes and culex (culicidae) that are detected in tropical and subtropical areas. main flaviviruses of public health importance are: dengue, west nile virus, yellow fever, among others. in continental chile, flaviviruses has not been detected. however, there are indigenous cases of dengue detected in easter island since 2002, as the presence of its vector aedes aegypti. the aim of this study was: to determine diversity o ... | 2015 | 25860055 |
| ovicidal and adulticidal potential of leaf and seed extract of albizia lebbeck (l.) benth. (family: fabaceae) against culex quinquefasciatus, aedes aegypti, and anopheles stephensi (diptera: culicidae). | several diseases are associated to the mosquito-human interaction. mosquitoes are the carriers of severe and well-known illnesses such as malaria, arboviral encephalitis, dengue fever, chikungunya fever, west nile virus, and yellow fever. these diseases produce significant morbidity and mortality in humans and livestock around the world. in the present study, hexane, benzene, chloroform, ethyl acetate, and methanol extracts of leaf and seed of albizia lebbeck were assayed for their toxicity agai ... | 2015 | 25681143 |
| laboratory evaluation of indian medicinal plants as repellents against malaria, dengue, and filariasis vector mosquitoes. | mosquito-borne diseases have an economic impact, including loss in commercial and labor outputs, particularly in countries with tropical and subtropical climates; however, no part of the world is free from vector-borne diseases. mosquitoes are the carriers of severe and well-known illnesses such as malaria, arboviral encephalitis, dengue fever, chikungunya fever, west nile virus, and yellow fever. these diseases produce significant morbidity and mortality in humans and livestock around the world ... | 2015 | 25399815 |
| dengue fever in china: an emerging problem demands attention. | 2015 | 26038760 | |
| trade-off between thermal tolerance and insecticide resistance in plutella xylostella. | fitness costs associated with resistance to insecticides have been well documented, usually at normal temperature conditions, in many insect species. in this study, using chlorpyrifos-resistant homozygote (rr) and chlorpyrifos-susceptible homozygote (ss) of resistance ace1 allele of plutella xylostella (dbm), we confirmed firstly that high temperature experience in pupal stage influenced phenotype of wing venation in insecticide-resistant and insecticide-susceptible plutella xylostella, and ss d ... | 2015 | 25691976 |
| choroidal neovascularization induced by immunogenic alteration of the retinal pigment epithelium in dengue fever. | to report the first case of choroidal neovascularization (cnv) secondary to dengue fever. | 2015 | 25759665 |
| investigation of mosquito oviposition pheromone as lethal lure for the control of aedes aegypti (l.) (diptera: culicidae). | the trend in chemical insecticide development has focused on improving the efficacy against mosquitoes while reducing the environmental impact. lethal lures apply an "attract-and-kill" strategy that draws the insect to the killing agent rather than bringing the killing agent to the insect. | 2015 | 25588346 |
| daily forecast of dengue fever incidents for urban villages in a city. | instead of traditional statistical models for large spatial areas and weekly or monthly temporal units, what public health workers urgently need is a timely risk prediction method for small areas. this risk prediction would provide information for early warning, target surveillance and intervention. | 2015 | 25636965 |
| identification of plant compounds that disrupt the insect juvenile hormone receptor complex. | insects impact human health through vector-borne diseases and cause major economic losses by damaging crops and stored agricultural products. insect-specific growth regulators represent attractive control agents because of their safety to the environment and humans. we identified plant compounds that serve as juvenile hormone antagonists (pjhans). using the yeast two-hybrid system transformed with the mosquito jh receptor as a reporter system, we demonstrate that pjhans affect the jh receptor, m ... | 2015 | 25624480 |
| horizontal transmission of an r4 clade non-long terminal repeat retrotransposon between the divergent aedes and anopheles mosquito genera. | aaegr4_1 and agamr4_1 are the sole r4 clade non-long terminal repeat (non-ltr) retrotransposons in aedes aegypti and anopheles gambiae, two species that diverged approximately 145-200 million years ago. twelve full-length copies were found in ae. aegypti and have less than 1% nucleotide (nt) divergence, suggesting recent activity on an evolutionary time scale. five of these copies have intact open reading frames and the 3.6 kb open reading frame of aaegr4_1.1 has 78% nt identity to agamr4_1.1. n ... | 2015 | 25615532 |
| standard operating procedures for standardized mass rearing of the dengue and chikungunya vectors aedes aegypti and aedes albopictus (diptera: culicidae) - i - egg quantification. | quantification of eggs prior to rearing the immature stages of mosquitoes is an essential step in establishing a standardized mass rearing system. to develop a simple and accurate method of egg quantification for aedes aegypti and aedes albopictus, the relationship between egg number and weight, as well as egg number and volume, were studied. | 2015 | 25614052 |
| microrna-8 targets the wingless signaling pathway in the female mosquito fat body to regulate reproductive processes. | female mosquitoes require a blood meal for reproduction, and this blood meal provides the underlying mechanism for the spread of many important vector-borne diseases in humans. a deeper understanding of the molecular mechanisms linked to mosquito blood meal processes and reproductive events is of particular importance for devising innovative vector control strategies. we found that the conserved microrna mir-8 is an essential regulator of mosquito reproductive events. two strategies to inhibit m ... | 2015 | 25605933 |
| insecticide-mediated up-regulation of cytochrome p450 genes in the red flour beetle (tribolium castaneum). | some cytochrome p450 (cyp) genes are known for their rapid up-regulation in response to insecticide exposures in insects. to date, however, limited information is available with respect to the relationships among the insecticide type, insecticide concentration, exposure duration and the up-regulated cyp genes. in this study, we examined the transcriptional response of eight selected cyp genes, including cyp4g7, cyp4q4, cyp4br3, cyp12h1, cyp6bk11, cyp9d4, cyp9z5 and cyp345a1, to each of four inse ... | 2015 | 25607733 |
| the abcs of eye color in tribolium castaneum: orthologs of the drosophila white, scarlet, and brown genes. | in drosophila melanogaster, each of the three paralogous abc transporters, white, scarlet and brown, is required for normal pigmentation of the compound eye. we have cloned the three orthologous genes from the beetle tribolium castaneum. conceptual translations of tribolium white (tcw), scarlet (tcst), and brown (tcbw) are 51, 48, and 32% identical to their respective drosophila counterparts. we have identified loss-of-eye-pigment strains that bear mutations in tcw and tcst: the tcw gene in the ... | 2015 | 25555987 |
| a novel mechanism of latency in matrix metalloproteinases. | the matrix metalloproteinases (mmps) are a family of secreted soluble or membrane-anchored multimodular peptidases regularly found in several paralogous copies in animals and plants, where they have multiple functions. the minimal consensus domain architecture comprises a signal peptide, a 60-90-residue globular prodomain with a conserved sequence motif including a cysteine engaged in "cysteine-switch" or "velcro" mediated latency, and a catalytic domain. karilysin, from the human periodontopath ... | 2015 | 25555916 |
| uncovering the novel characteristics of asian honey bee, apis cerana, by whole genome sequencing. | the honey bee is an important model system for increasing understanding of molecular and neural mechanisms underlying social behaviors relevant to the agricultural industry and basic science. the western honey bee, apis mellifera, has served as a model species, and its genome sequence has been published. in contrast, the genome of the asian honey bee, apis cerana, has not yet been sequenced. a. cerana has been raised in asian countries for thousands of years and has brought considerable economic ... | 2015 | 25553907 |
| annotation and expression analysis of cuticular proteins from the tobacco hornworm, manduca sexta. | the insect cuticle is a unique material that covers the exterior of the animal as well as lining the foregut, hindgut, and tracheae. it offers protection from predators and desiccation, defines body shape, and serves as an attachment site for internal organs and muscle. it has demonstrated remarkable variations in hardness, flexibility and elasticity, all the while being light weight, which allows for ease of movement and flight. it is composed primarily of chitin, proteins, catecholamines, and ... | 2015 | 25576653 |
| tsetse gmmsrpn10 has anti-complement activity and is important for successful establishment of trypanosome infections in the fly midgut. | the complement cascade in mammalian blood can damage the alimentary tract of haematophagous arthropods. as such, these animals have evolved their own repertoire of complement-inactivating factors, which are inadvertently exploited by blood-borne pathogens to escape complement lysis. unlike the bloodstream stages, the procyclic (insect) stage of trypanosoma brucei is highly susceptible to complement killing, which is puzzling considering that a tsetse takes a bloodmeal every 2-4 days. in this stu ... | 2015 | 25569180 |
| pyrethroid-resistance and presence of two knockdown resistance (kdr) mutations, f1534c and a novel mutation t1520i, in indian aedes aegypti. | control of aedes aegypti, the mosquito vector of dengue, chikungunya and yellow fever, is a challenging task. pyrethroid insecticides have emerged as a preferred choice for vector control but are threatened by the emergence of resistance. the present study reports a focus of pyrethroid resistance and presence of two kdr mutations--f1534c and a novel mutation t1520i, in ae. aegypti from delhi, india. | 2015 | 25569164 |
| origins of context-dependent gene repression by capicua. | receptor tyrosine kinase (rtk) signaling pathways induce multiple biological responses, often by regulating the expression of downstream genes. the hmg-box protein capicua (cic) is a transcriptional repressor that is downregulated in response to rtk signaling, thereby enabling rtk-dependent induction of cic targets. in both drosophila and mammals, cic is expressed as two isoforms, long (cic-l) and short (cic-s), whose functional significance and mechanism of action are not well understood. here ... | 2015 | 25569482 |
| engineered mosquitoes to fight mosquito borne diseases: not a merely technical issue. | malaria, dengue and other mosquito-borne diseases pose dramatic problems of public health, particularly in tropical and sub-tropical countries. historically, vector control has been one of the most successfully strategies to eradicate some mosquito-borne diseases, as witnessed by malaria eradication in mediterranean regions such as italy and greece. vector control through insecticides has been used worldwide; unfortunately, it is losing effectiveness due to spread of resistances. control of mosq ... | 2015 | 25495663 |
| the molecular and immunochemical expression of innexins in the yellow fever mosquito, aedes aegypti: insights into putative life stage- and tissue-specific functions of gap junctions. | gap junctions (gj) mediate direct intercellular communication by forming channels through which certain small molecules and/or ions can pass. connexins, the proteins that form vertebrate gj, are well studied and known to contribute to neuronal, muscular and epithelial physiology. innexins, the gj proteins of insects, have only recently received much investigative attention and many of their physiological roles remain to be determined. here we characterize the molecular expression of six innexin ... | 2015 | 25585357 |
| a genome-wide analysis of antimicrobial effector genes and their transcription patterns in manduca sexta. | antimicrobial proteins/peptides (amps) are effectors of innate immune systems against pathogen infection in multicellular organisms. over half of the amps reported so far come from insects, and these effectors act in concert to suppress or kill bacteria, fungi, viruses, and parasites. in this work, we have identified 86 amp genes in the manduca sexta genome, most of which seem likely to be functional. they encode 15 cecropins, 6 moricins, 6 defensins, 3 gallerimycins, 4 x-tox splicing variants, ... | 2015 | 25662101 |
| bacillus thuringiensis subsp. israelensis producing endochitinase chia74δsp inclusions and its improved activity against aedes aegypti. | the objective of this study was to produce stable inclusions of chitinase chia74δsp in bacillus thuringiensis subsp. israelensis (bti) and to assay its insecticidal activity against aedes aegypti larvae. | 2015 | 26434743 |
| s argassum muticum-synthesized silver nanoparticles: an effective control tool against mosquito vectors and bacterial pathogens. | mosquito-borne diseases represent a deadly threat for millions of people worldwide. furthermore, pathogens and parasites polluting water also constitute a severe plague for populations of developing countries. in this research, silver nanoparticles (agnp) were synthesized using the aqueous extract of the seaweed sargassum muticum. the production of agnp was confirmed by surface plasmon resonance band illustrated in uv-vis spectrophotometry. agnp were characterized by ftir, sem, edx, and xrd anal ... | 2015 | 26281786 |
| extracellular synthesis of silver nanoparticles using bacillus megaterium against malarial and dengue vector (diptera: culicidae). | biosynthesis of silver nanoparticles has provoked nowadays and alternative to physical and chemical approaches. in the present study, silver nanoparticles (agnps) were synthesized extracellular method using bacillus megaterium. the agnps formations were confirmed initially through color change, and the aliquots were characterized through uv-visible spectrophotometer, followed by scanning electron microscopy (sem), energy-dispersive x-ray (edx) spectroscopy, and fourier transform infrared (ftir) ... | 2015 | 26231835 |
| [ecology of aedes aegypti and aedes albopictus in the americas and disease transmission]. | the recent range expansion of the mosquito aedes albopictus has been associated in some areas by declines in abundance or local elimination of aedes aegypti, but the two species still coexist in large regions of the americas. we offer a summary of the possible mechanisms responsible for the abundance and displacement pattern observed and of their significance in terms of disease transmission. among these mechanisms we may mention the competition for limiting resources, the differences in the abi ... | 2015 | 26535539 |
| insecticidal potency of aspergillus terreus against larvae and pupae of three mosquito species anopheles stephensi, culex quinquefasciatus, and aedes aegypti. | microbial control agents offer alternatives to chemical pest control, as they can be more selective than chemical insecticides. the present study evaluates the mosquito larvicidal and pupicidal potential of fungus mycelia using ethyl acetate and methanol solvent extracts produced by aspergillus terreus against anopheles stephensi, culex quinquefasciatus, and aedes aegypti. the a. terreus mycelia were extracted after 15 days from sabouraud dextrose broth medium. the ethyl acetate extracts showed ... | 2015 | 26139412 |
| genome mining offers a new starting point for parasitology research. | parasites including helminthes, protozoa, and medical arthropod vectors are a major cause of global infectious diseases, affecting one-sixth of the world's population, which are responsible for enormous levels of morbidity and mortality important and remain impediments to economic development especially in tropical countries. prevalent drug resistance, lack of highly effective and practical vaccines, as well as specific and sensitive diagnostic markers are proving to be challenging problems in p ... | 2015 | 25563615 |
| [effect of bacillus thuringiensis var. israelensis (bti) on detoxification enzyme activity of larvae of culex pipiens pallens and aedes aegypti]. | to investigate the effect of bacillus thuringiensis var. israelensis (bti) on the activities of three detoxification enzymes of culex pipiens pallens and aedes aegypti larvae. | 2015 | 26767261 |
| evaluating liquid and granular bacillus thuringiensis var. israelensis broadcast applications for controlling vectors of dengue and chikungunya viruses in artificial containers and tree holes. | the principal vectors of chikungunya and dengue viruses typically oviposit in water-filled artificial and natural containers, including tree holes. despite the risk these and similar tree hole-inhabiting mosquitoes present to global public health, surprisingly few studies have been conducted to determine an efficient method of applying larvicides specifically to tree holes. the stihl sr 450, a backpack sprayer commonly utilized during military and civilian vector control operations, may be suita ... | 2015 | 26335473 |
| susceptibility profile of aedes aegypti from santiago island, cabo verde, to insecticides. | in 2009, cabo verde diagnosed the first dengue cases, with 21,137 cases reported and aedes aegypti was identified as the vector. since the outbreak, chemical insecticides and source reduction were used to control the mosquito population. this study aimed to assess the susceptibility of a. aegypti populations from santiago, cabo verde to insecticides and identify the mechanisms of resistance. samples of a. aegypti eggs were obtained at two different time periods (2012 and 2014), using ovitraps in ... | 2015 | 26307496 |
| the genetic architecture of a complex trait: resistance to multiple toxins produced by bacillus thuringiensis israelensis in the dengue and yellow fever vector, the mosquito aedes aegypti. | the bacterial insecticide bacillus thuringiensis subsp. israelensis (bti) is an increasingly popular alternative to chemical insecticides for controlling mosquito populations. because bti toxicity relies on the action of four main toxins, resistance to bti is very likely a complex phenotype involving several genes simultaneously. dissecting the underlying genetic basis thus requires associating a quantitative measure of resistance to genetic variation at many loci in a segregating population. he ... | 2015 | 26238211 |
| cry4ba and cyt1aa proteins from bacillus thuringiensis israelensis: interactions and toxicity mechanism against aedes aegypti. | individual crystal proteins from bacillus thuringiensis israelensis exhibit variable levels of insecticidal activities against mosquito larvae. in all cases, they are much less active compared to the whole crystal proteins due to described complex synergistic interactions among them. in the present study we investigated the effects of cyt1a98 (a cyt1aa type protein) on cry4blb (a cry4ba type toxin) insecticidal activity toward the dengue vector aedes aegypti. the bioassay analyses demonstrated t ... | 2015 | 26238170 |
| evidence of two mechanisms involved in bacillus thuringiensis israelensis decreased toxicity against mosquito larvae: genome dynamic and toxins stability. | biopesticides based on bacillus thuringiensis israelensis are the most used and most successful around the world. this bacterium is characterized by a dynamic genome able to win or lose genetic materials which leads to a decrease in its effectiveness. the detection of such phenomena is of great importance to monitor the stability of b. thuringiensis strains in industrial production processes of biopesticides. new local b. thuringiensis israelensis isolates were investigated. they present variabl ... | 2015 | 26070692 |
| effects of the p20 protein from bacillus thuringiensis israelensis on insecticidal crystal protein cry4ba. | the accessory protein p20 from bacillus thuringiensis israelensis has been defined as an important molecular chaperone for forming crystal cyt1aa, and enhancing cry11aa and cry4aa expression. to investigate its putative role in cry4ba delta-endotoxin production and toxicity, a p20 gene was cloned and introduced into b. thuringiensis recombinant strain expressing cry4ba type gene (cry4blb). the delta-endotoxin synthesis was enhanced by 262%. the generated inclusions were assayed against third ins ... | 2015 | 25931398 |
| two specific membrane-bound aminopeptidase n isoforms from aedes aegypti larvae serve as functional receptors for the bacillus thuringiensis cry4ba toxin implicating counterpart specificity. | the interaction between bacillus thuringiensis cry toxins and their receptors on midgut cells of susceptible insect larvae is the critical determinant in toxin specificity. besides gpi-linked alkaline phosphatase in aedes aegypti mosquito-larval midguts, membrane-bound aminopeptidase n (aaeapn) is widely thought to serve as a cry4ba receptor. here, two full-length aaeapn isoforms, aaeapn2778 and aaeapn2783, predicted to be gpi-linked were cloned and successfully expressed in spodoptera frugiperd ... | 2015 | 25871797 |
| maintenance of residual activity of bt toxin by using natural and synthetic dyes: a novel approach for sustainable mosquito vector control. | mosquito control protein from bacillus thuringiensis gets inactivated with exposure to sunlight. to address this issue, the potential of synthetic and natural dye was investigated as sunlight protectants. bt sv2 in absence of dyes when exposed to sunlight showed reduced effectiveness against the fourth instars of mosquito larvae. whereas acriflavin, congo red and violacein were able to maintain 86.4%, 91.6% and 82.2% mosquito larvicidal efficacy of bt sv2 against ivth instars larvae of anopheles ... | 2015 | 25699646 |
| functional assembly of 260-kda oligomers required for mosquito-larvicidal activity of the bacillus thuringiensis cry4ba toxin. | oligomerization has been shown to contribute to the toxicity of bacillus thuringiensis cry toxins. mutations have been made in the cry4ba toxin and resulted in toxic to non-toxic mutants toward aedes aegypti larvae. in this study, cry4ba wild type and mutants were analyzed for oligomer formation in vitro, biochemical properties and their relationships with larvicidal activity. in vitro, the cry4ba forms two-main types of the oligomers including (1) the 260-kda and larger oligomers, which assembl ... | 2015 | 25687547 |
| characterisation of novel bacillus thuringiensis isolates against aedes aegypti (diptera: culicidae) and ceratitis capitata (diptera: tephridae). | bacillus thuringiensis is successfully used in pest management strategies as an eco-friendly bioinsecticide. isolation and identification of new strains with a wide variety of target pests is an ever growing field. in this paper, new b. thuringiensis isolates were investigated to search for original strains active against diptera and able to produce novel toxins that could be used as an alternative for the commercial h14 strain. biochemical and molecular characterization revealed a remarkable di ... | 2015 | 25433312 |
| [vital study of the fatty body of larvae of aedes aegypti l. infected with densonucleosis virus]. | 2015 | 67557 |