Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| alternative nf-κb isoforms in the drosophila neuromuscular junction and brain. | the drosophila nf-κb protein dorsal is expressed at the larval neuromuscular junction, where its expression appears unrelated to known dorsal functions in embryonic patterning and innate immunity. using confocal microscopy with domain-specific antisera, we demonstrate that larval muscle expresses only the b isoform of dorsal, which arises by intron retention. we find that dorsal b interacts with and stabilizes cactus at the neuromuscular junction, but exhibits cactus independent localization and ... | 2015 | 26167685 |
| stimulating anopheles gambiae swarms in the laboratory: application for behavioural and fitness studies. | male anopheles mosquitoes that swarm rely in part on features of the environment including visual stimuli to locate swarms. swarming is believed to be the primary behaviour during which mating occurs in the field, but is not a common behaviour in the laboratory. features that stimulate male anopheles gambiae g3 strain swarming were created in novel large indoor cages. | 2015 | 26169677 |
| the performance of the sd bioline dengue duo® rapid immunochromatographic test kit for the detection of ns1 antigen, igm and igg antibodies during a dengue type 1 epidemic in jamaica. | dengue is an important mosquito-borne viral infection that affects millions of persons worldwide. early diagnosis is necessary to effect appropriate management and decrease mortality. immunochromatographic tests are advantageous in producing dengue test results within 30 min but these results should be sensitive and specific. in this study we evaluated the diagnostic performance of the sd bioline dengue duo® rapid immunochromatographic test kit. a panel of 309 dengue and 30 non-dengue single ser ... | 2015 | 26173484 |
| high nucleotide diversity and limited linkage disequilibrium in helicoverpa armigera facilitates the detection of a selective sweep. | insecticides impose extreme selective pressures on populations of target pests and so insecticide resistance loci of these species may provide the footprints of 'selective sweeps'. to lay the foundation for future genome-wide scans for selective sweeps and inform genome-wide association study designs, we set out to characterize some of the baseline population genomic parameters of one of the most damaging insect pests in agriculture worldwide, helicoverpa armigera. to this end, we surveyed nine ... | 2015 | 26174024 |
| in-depth characterization of trypsin-like serine peptidases in the midgut of the sugar fed culex quinquefasciatus. | culex quinquefasciatus is a hematophagous insect from the culicidae family that feeds on the blood of humans, dogs, birds and livestock. this species transmits a wide variety of pathogens between humans and animals. the midgut environment is the first location of pathogen-vector interactions for blood-feeding mosquitoes and the expression of specific peptidases in the early stages of feeding could influence the outcome of the infection. trypsin-like serine peptidases belong to a multi-gene famil ... | 2015 | 26174750 |
| the global compendium of aedes aegypti and ae. albopictus occurrence. | aedes aegypti and ae. albopictus are the main vectors transmitting dengue and chikungunya viruses. despite being pathogens of global public health importance, knowledge of their vectors' global distribution remains patchy and sparse. a global geographic database of known occurrences of ae. aegypti and ae. albopictus between 1960 and 2014 was compiled. herein we present the database, which comprises occurrence data linked to point or polygon locations, derived from peer-reviewed literature and un ... | 2015 | 26175912 |
| web platform using digital image processing and geographic information system tools: a brazilian case study on dengue. | dengue fever is endemic in asia, the americas, the east of the mediterranean and the western pacific. according to the world health organization, it is one of the diseases of greatest impact on health, affecting millions of people each year worldwide. a fast detection of increases in populations of the transmitting vector, the aedes aegypti mosquito, is essential to avoid dengue outbreaks. unfortunately, in several countries, such as brazil, the current methods for detecting populations changes ... | 2015 | 26178732 |
| pest control and resistance management through release of insects carrying a male-selecting transgene. | development and evaluation of new insect pest management tools is critical for overcoming over-reliance upon, and growing resistance to, synthetic, biological and plant-expressed insecticides. for transgenic crops expressing insecticidal proteins from the bacterium bacillus thuringiensis ('bt crops') emergence of resistance is slowed by maintaining a proportion of the crop as non-bt varieties, which produce pest insects unselected for resistance. while this strategy has been largely successful, ... | 2015 | 26179401 |
| mechanism of ammonia excretion in the freshwater leech nephelopsis obscura: characterization of a primitive rh protein and effects of high environmental ammonia. | remarkably little is known about nitrogenous excretion in freshwater invertebrates. in the current study, the nitrogen excretion mechanism in the carnivorous ribbon leech, nephelopsis obscura, was investigated. excretion experiments showed that the ribbon leech is ammonotelic, excreting 166.0 ± 8.6 nmol·grams fresh weight (gfw)(-1)·h(-1) ammonia and 14.7 ± 1.9 nmol·gfw(-1)·h(-1) urea. exposure to high and low ph hampered and enhanced, respectively, ammonia excretion rates, indicating an acid-lin ... | 2015 | 26180186 |
| the hidden burden of dengue and chikungunya in chennai, india. | dengue and chikungunya are rapidly expanding viruses transmitted by mosquitoes of the genus aedes. few epidemiological studies have examined the extent of transmission of these infections in south india despite an increase in the number of reported cases, and a high suitability for transmission. | 2015 | 26181441 |
| bacterial exposure at the larval stage induced sexual immune dimorphism and priming in adult aedes aegypti mosquitoes. | gender differences in the immune response of insects are driven by natural selection for females and sexual selection for males. these natural forces entail a multitude of extrinsic and intrinsic factors involved in a genotype-environment interaction that results in sex-biased expression of the genes shared by males and females. however, little is known about how an infection at a particular ontogenetic stage may influence later stages, or how it may impact sexual immune dimorphism. using aedes ... | 2015 | 26181517 |
| new state records of mosquitoes for colorado. | the 1967 treatment of the mosquitoes of colorado by harmston and lawson and subsequent publications have recorded 46 culicid species from colorado. as part of a study to create an updated synopsis of the mosquitoes of colorado, adult trapping at numerous localities was conducted in colorado during the summers of 2013 and 2014. this review also included an examination of mosquito specimens in various relevant museum collections. aedes (ochlerotatus) niphadopsis and ae. (och.) spencerii spencerii ... | 2015 | 26181688 |
| evaluation of three commercial backpack sprayers with aqualuer® 20-20 against caged adult aedes aegypti. | three commercially available backpack sprayers were evaluated with aqualuer® 20-20 (20.6% permethrin, active ingredient; 20.6% piperonyl butoxide, technical) against caged adult aedes aegypti in semifield trials in northeastern florida. two battery-powered sprayers, birchmeier and hudson, were compared with the standard hand-pump solo 425 sprayer, which is currently used in pest management operations. physical characteristics, droplet analysis, and overall ease of use were documented. multiple d ... | 2015 | 26181698 |
| annona muricata (annonaceae): a review of its traditional uses, isolated acetogenins and biological activities. | annona muricata is a member of the annonaceae family and is a fruit tree with a long history of traditional use. a. muricata, also known as soursop, graviola and guanabana, is an evergreen plant that is mostly distributed in tropical and subtropical regions of the world. the fruits of a. muricata are extensively used to prepare syrups, candies, beverages, ice creams and shakes. a wide array of ethnomedicinal activities is contributed to different parts of a. muricata, and indigenous communities ... | 2015 | 26184167 |
| tissue barriers to arbovirus infection in mosquitoes. | arthropod-borne viruses (arboviruses) circulate in nature between arthropod vectors and vertebrate hosts. arboviruses often cause devastating diseases in vertebrate hosts, but they typically do not cause significant pathology in their arthropod vectors. following oral acquisition of a viremic bloodmeal from a vertebrate host, the arbovirus disease cycle requires replication in the cellular environment of the arthropod vector. once the vector has become systemically and persistently infected, the ... | 2015 | 26184281 |
| characterization and biotoxicity of hypnea musciformis-synthesized silver nanoparticles as potential eco-friendly control tool against aedes aegypti and plutella xylostella. | two of the most important challenges facing humanity in the 21st century comprise food production and disease control. eco-friendly control tools against mosquito vectors and agricultural pests are urgently needed. insecticidal products of marine origin have a huge potential to control these pests. in this research, we reported a single-step method to synthesize silver nanoparticles (agnp) using the aqueous leaf extract of the seaweed hypnea musciformis, a cheap, nontoxic and eco-friendly materi ... | 2015 | 26184431 |
| complete genome sequence of chikungunya virus isolated from an aedes aegypti mosquito during an outbreak in yemen, 2011. | chikungunya virus is recognized as a serious public health problem. the complete genome was sequenced for a chikungunya virus isolated from the mosquito aedes aegypti during a 2011 outbreak in al hodayda, yemen, which resulted in significant human fatalities. phylogenetic analysis demonstrated that this yemeni isolate is most closely related to indian ocean strains of the east/central/south african genotype. | 2015 | 26184944 |
| an insight into the functional role of thioredoxin reductase, a selenoprotein, in maintaining normal native microbiota in the gulf coast tick (amblyomma maculatum). | tick selenoproteins have been associated with antioxidant activity in ticks. thioredoxin reductase (trxr), also a selenoprotein, belongs to the pyridine nucleotide-disulphide oxidoreductase family of proteins and is an important antioxidant. molecular interactions between native microbiota and tick hosts have barely been investigated to date. in this study, we determined the functional role of trxr in tick feeding and in maintenance of the native microbial community. trxr transcript levels remai ... | 2015 | 26184979 |
| the efficacy of chinese medicinal herbs towards grape phylloxera (daktulosphaira vitifoliae fitch) (hemiptera, phylloxeridae). | bio-insecticidal effects of seven chinese medicinal herbs on mortality, fecundity, developmental periods and life table parameters of the grape phylloxera were investigated. in an excised root bioassay experiment aqueous extracts from seven chinese medicinal herbs increased grape phylloxera first instar mortality (26.00-38.50%) compared to other instars. the intrinsic rate of increase (rm), finite rate of increase (λ), fecundity rate and net reproductive rate (r0) were significantly reduced by a ... | 2015 | 26186216 |
| mapping of the sequences directing localization of the drosophila germ cell-expressed protein (gce). | drosophila melanogaster germ cell-expressed protein (gce) belongs to the family of bhlh-pas transcription factors that are the regulators of gene expression networks that determine many physiological and developmental processes. gce is a homolog of d. melanogaster methoprene tolerant protein (met), a key mediator of anti-metamorphic signaling in insects and the putative juvenile hormone receptor. recently, it has been shown that the functions of met and gce are only partially redundant and tissu ... | 2015 | 26186223 |
| a new membrane lipid raft gene spflt-1 facilitating the endocytosis of vibrio alginolyticus in the crab scylla paramamosain. | pathogens can enter their host cells by way of endocytosis in which the membrane lipid raft gene flotillins are probably involved in the invasion process and this is an important way to cause infection. in this study, a new gene spflt-1 was identified in scylla paramamosain, which shared high identity with the flotillin-1 of other species. the spflt-1 gene was widely distributed in tissues and showed the highest level of mrna transcripts in the hemocytes. this gene might be a maternal gene based ... | 2015 | 26186350 |
| nuclease tudor-sn is involved in tick dsrna-mediated rna interference and feeding but not in defense against flaviviral or anaplasma phagocytophilum rickettsial infection. | tudor staphylococcal nuclease (tudor-sn) and argonaute (ago) are conserved components of the basic rna interference (rnai) machinery with a variety of functions including immune response and gene regulation. the rnai machinery has been characterized in tick vectors of human and animal diseases but information is not available on the role of tudor-sn in tick rnai and other cellular processes. our hypothesis is that tick tudor-sn is part of the rnai machinery and may be involved in innate immune r ... | 2015 | 26186700 |
| predicting scenarios for successful autodissemination of pyriproxyfen by malaria vectors from their resting sites to aquatic habitats; description and simulation analysis of a field-parameterizable model. | large-cage experiments indicate pyriproxifen (ppf) can be transferred from resting sites to aquatic habitats by anopheles arabiensis--malaria vector mosquitoes to inhibit emergence of their own offspring. ppf coverage is amplified twice: (1) partial coverage of resting sites with ppf contamination results in far higher contamination coverage of adult mosquitoes because they are mobile and use numerous resting sites per gonotrophic cycle, and (2) even greater contamination coverage of aquatic hab ... | 2015 | 26186730 |
| a corpora allata farnesyl diphosphate synthase in mosquitoes displaying a metal ion dependent substrate specificity. | farnesyl diphosphate synthase (fpps) is a key enzyme in isoprenoid biosynthesis, it catalyzes the head-to-tail condensation of dimethylallyl diphosphate (dmapp) with two molecules of isopentenyl diphosphate (ipp) to generate farnesyl diphosphate (fpp), a precursor of juvenile hormone (jh). in this study, we functionally characterized an aedes aegypti fpps (aafpps) expressed in the corpora allata. aafpps is the only fpps gene present in the genome of the yellow fever mosquito, it encodes a 49.6 k ... | 2015 | 26188328 |
| mosquitoes use vision to associate odor plumes with thermal targets. | all moving animals, including flies, sharks, and humans, experience a dynamic sensory landscape that is a function of both their trajectory through space and the distribution of stimuli in the environment. this is particularly apparent for mosquitoes, which use a combination of olfactory, visual, and thermal cues to locate hosts. mosquitoes are thought to detect suitable hosts by the presence of a sparse co₂ plume, which they track by surging upwind and casting crosswind. upon approach, local cu ... | 2015 | 26190071 |
| tree holes as larval habitats for aedes aegypti in urban, suburban and forest habitats in a dengue affected area. | aedes aegypti (l.) (diptera: culicidae), the main vector of dengue and urban yellow fever in the world, is highly adapted to the human environment. artificial containers are the most common larval habitat for the species, but it may develop in tree holes and other phytotelmata. this study assessed whether tree holes in san ramón de la nueva orán, a city located in subtropical montane moist forest where dengue outbreaks occur, are relevant as larval habitat for ae. aegypti and if the species may ... | 2015 | 26193903 |
| metazoan trna introns generate stable circular rnas in vivo. | we report the discovery of a class of abundant circular noncoding rnas that are produced during metazoan trna splicing. these transcripts, termed trna intronic circular (tric)rnas, are conserved features of animal transcriptomes. biogenesis of tricrnas requires anciently conserved trna sequence motifs and processing enzymes, and their expression is regulated in an age-dependent and tissue-specific manner. furthermore, we exploited this biogenesis pathway to develop an in vivo expression system f ... | 2015 | 26194134 |
| nonfunctional ingestion of plant mirnas in silkworm revealed by digital droplet pcr and transcriptome analysis. | since a plant mirna (mir168) cross-regulating a mammalian transcript was reported, mirna-mediated cross-kingdom communication has become one of the most compelling but controversial topics. in the present study, we used silkworm and mulberry, which is a model for studies on the interactions between the insect and its host plant, to address whether mirna-mediated cross-kingdom communication is a common phenomenon. the results of ta clone, sanger sequencing and droplet digital pcr demonstrated tha ... | 2015 | 26195298 |
| long-lasting permethrin-impregnated clothing protects against mosquito bites in outdoor workers. | outdoor exposure to mosquitoes is a risk factor for many diseases, including malaria and dengue. we have previously shown that long-lasting permethrin-impregnated clothing protects against tick and chigger bites in a double-blind randomized controlled trial in north carolina outdoor workers. here, we evaluated whether this clothing is protective against mosquito bites by measuring changes in antibody titers to mosquito salivary gland extracts. on average, there was a 10-fold increase in titer du ... | 2015 | 26195460 |
| detection of chikungunya virus in nepal. | chikungunya virus (chikv) is an emerging alphaviral disease and a public health problem in south asia including nepal in recent years. in this study, sera were collected from patients presenting with fever, headache, muscular pain, fatigue, and joint pain of both upper and lower extremities. a total of 169 serum samples were tested for chikv and dengue virus (denv) by using immunoglobulin m (igm) and immunoglobulin g (igg) antibody using enzyme-linked immunosorbent assay (elisa) method during au ... | 2015 | 26195462 |
| underrecognition of dengue during 2013 epidemic in luanda, angola. | during the 2013 dengue epidemic in luanda, angola, 811 dengue rapid diagnostic test-positive cases were reported to the ministry of health. to better understand the magnitude of the epidemic and identify risk factors for dengue virus (denv) infection, we conducted cluster surveys around households of case-patients and randomly selected households 6 weeks after the peak of the epidemic. of 173 case cluster participants, 16 (9%) exhibited evidence of recent denv infection. of 247 random cluster pa ... | 0 | 26196224 |
| stress responses of small heat shock protein genes in lepidoptera point to limited conservation of function across phylogeny. | the small heat shock protein (shsp) family is thought to play an important role in protein refolding and signal transduction, and thereby protect organisms from stress. however little is known about shsp function and conservation across phylogenies. in the current study, we provide a comprehensive assessment of small hsp genes and their stress responses in the oriental fruit moth (ofm), grapholita molesta. fourteen small heat shock proteins of ofm clustered with related hsps in other lepidoptera ... | 2015 | 26196395 |
| spatial distribution of dengue in a brazilian urban slum setting: role of socioeconomic gradient in disease risk. | few studies of dengue have shown group-level associations between demographic, socioeconomic, or geographic characteristics and the spatial distribution of dengue within small urban areas. this study aimed to examine whether specific characteristics of an urban slum community were associated with the risk of dengue disease. | 2015 | 26196686 |
| scrutinizing the immune defence inventory of camponotus floridanus applying total transcriptome sequencing. | defence mechanisms of organisms are shaped by their lifestyle, environment and pathogen pressure. carpenter ants are social insects which live in huge colonies comprising genetically closely related individuals in high densities within nests. this lifestyle potentially facilitates the rapid spread of pathogens between individuals. in concert with their innate immune system, social insects may apply external immune defences to manipulate the microbial community among individuals and within nests. ... | 2015 | 26198742 |
| biocontrol evaluation of extracts and a major component, clusianone, from clusia fluminensis planch. & triana against aedes aegypti. | studies evaluated the effects of hexanic extracts from the fruits and flowers of clusia fluminensis and the main component of the flower extract, a purified benzophenone (clusianone), against aedes aegypti. the treatment of larvae with the crude fruit or flower extracts from c. fluminensis did not affect the survival ofae. aegypti (50 mg/l), however, the flower extracts significantly delayed development of ae. aegypti. in contrast, the clusianone (50 mg/l) isolate from the flower extract, repres ... | 2015 | 26200711 |
| full-length infectious clone of a low passage dengue virus serotype 2 from brazil. | full-length dengue virus (denv) cdna clones are an invaluable tool for many studies, including those on the development of attenuated or chimeric vaccines and on host-virus interactions. furthermore, the importance of low passage denv infectious clones should be highlighted, as these may harbour critical and unique strain-specific viral components from field-circulating isolates. the successful construction of a functional brazilian low passage denv serotype 2 full-length clone through homologou ... | 2015 | 26200712 |
| reception of odors and repellents in mosquitoes. | mosquitoes use their sense of smell to find hosts, nectar, and oviposition sites, and to avoid repellents. a small number of mosquito species are adapted to feed on humans and have a major impact on public health by transmitting diseases such as malaria, dengue and filariasis. the application of odorants for behavioral control has not been fully realized yet due to complexity of the mosquito olfactory system. recent progress in molecular and computational tools has enabled rigorous investigation ... | 2015 | 26202080 |
| characterization of the honeybee amnav1 channel and tools to assess the toxicity of insecticides. | pollination is important for both agriculture and biodiversity. for a significant number of plants, this process is highly, and sometimes exclusively, dependent on the pollination activity of honeybees. the large numbers of honeybee colony losses reported in recent years have been attributed to colony collapse disorder. various hypotheses, including pesticide overuse, have been suggested to explain the disorder. using the xenopus oocytes expression system and two microelectrode voltage-clamp, we ... | 2015 | 26202396 |
| application of wmelpop wolbachia strain to crash local populations of aedes aegypti. | the endosymbiotic bacteria wolbachia pipientis (wmel strain) has been successfully established in several populations of aedes aegypti, the primary dengue vector. the virulent wolbachia strain wmelpop is known to cause several pathological impacts (increased egg mortality, life shortening, etc.) reducing overall fitness in the mosquito ae. aegypti. increased egg mortality could substantially reduce egg banks in areas with a lengthy monsoonal dry season, and be employed to eliminate local populat ... | 2015 | 26204449 |
| identifying genomic changes associated with insecticide resistance in the dengue mosquito aedes aegypti by deep targeted sequencing. | the capacity of mosquitoes to resist insecticides threatens the control of diseases such as dengue and malaria. until alternative control tools are implemented, characterizing resistance mechanisms is crucial for managing resistance in natural populations. insecticide biodegradation by detoxification enzymes is a common resistance mechanism; however, the genomic changes underlying this mechanism have rarely been identified, precluding individual resistance genotyping. in particular, the role of ... | 2015 | 26206155 |
| correction: evaluation of a push-pull approach for aedes aegypti (l.) using a novel dispensing system for spatial repellents in the laboratory and in a semi-field environment. | 2015 | 26207826 | |
| characterization of n-glycan structures on the surface of mature dengue 2 virus derived from insect cells. | denv envelope glycoprotein (e) is responsible for interacting with host cell receptors and is the main target for the development of a dengue vaccine based on an induction of neutralizing antibodies. it is well known that denv e glycoprotein has two potential n-linked glycosylation sites at asn67 and asn153. the n-glycans of e glycoprotein have been shown to influence the proper folding of the protein, its cellular localization, its interactions with receptors and its immunogenicity. however, th ... | 2015 | 26208004 |
| proteomic analysis reveals that cop9 signalosome complex subunit 7a (csn7a) is essential for the phase transition of migratory locust. | the migratory locust displays a reversible, density-dependent transition between the two phases of gregaria and solitaria. this phenomenon is a typical kind of behavior plasticity. here, we report that cop9 signalosome complex subunit 7a (csn7a) is involved in the regulation of locust phase transition. firstly, 90 proteins were identified to express differentially between the two phases by quantitative proteomic analysis. gregaria revealed higher levels in proteins related to structure formation ... | 2015 | 26212173 |
| a novel laminin β gene bmlanb1-w regulates wing-specific cell adhesion in silkworm, bombyx mori. | laminins are important basement membrane (bm) components with crucial roles in development. the numbers of laminin isoforms in various organisms are determined by the composition of the different α, β, and γ chains, and their coding genes, which are variable across spieces. in insects, only two α, one β, and one γ chains have been identified thus far. here, we isolated a novel laminin β gene, bmlanb1-w, by positional cloning of the mutant (crayfish, cf) with blistered wings in silkworm. gene str ... | 2015 | 26212529 |
| the seasonal reproduction number of dengue fever: impacts of climate on transmission. | background. dengue fever is a mosquito-borne viral disease and a regular epidemic in thailand. the peak of the dengue epidemic period is around june to august during the rainy season. it is believed that climate is an important factor for dengue transmission. method. a mathematical model for vector-host infectious disease was used to calculate the impacts of climate to the transmission of dengue virus. in this study, the data of climate and dengue fever cases were derived from chiang mai during ... | 2015 | 26213648 |
| mammalian cochlea as a physics guided evolution-optimized hearing sensor. | nonlinear physics plays an essential role in hearing. we demonstrate on a mesoscopic description level that during the evolutionary perfection of the hearing sensor, nonlinear physics led to the unique design of the cochlea observed in mammals, and that this design requests as a consequence the perception of pitch. our insight challenges the view that mostly genetics is responsible for the uniformity of the construction of the mammalian hearing sensor. our analysis also suggests that scaleable a ... | 2015 | 26216397 |
| economic burden of dengue virus infection at the household level among residents of puerto maldonado, peru. | dengue virus (denv) was reintroduced to peru in the 1990s and has been reported in puerto maldonado (population ~65,000) in the peruvian southern amazon basin since 2000. this region also has the highest human migration rate in the country, mainly from areas not endemic for denv. the objective of this study was to assess the proportion of household income that is diverted to costs incurred because of dengue illness and to compare these expenses between recent migrants (rms) and long-term residen ... | 2015 | 26217040 |
| in vitro and in vivo host range of anopheles gambiae densovirus (agdnv). | agdnv is a powerful gene transduction tool and potential biological control agent for anopheles mosquitoes. using a gfp reporter virus system, we investigated agdnv host range specificity in four arthropod cell lines (derived from an. gambiae, aedes albopictus and drosophila melanogaster) and six mosquito species from 3 genera (an. gambiae, an. arabiensis, an. stephensi, ae. albopictus, ae. aegypti and culex tarsalis). in vitro, efficient viral invasion, replication and gfp expression was only o ... | 2015 | 26220140 |
| transcriptional analysis of susceptible and resistant european corn borer strains and their response to cry1f protoxin. | despite a number of recent reports of insect resistance to transgenic crops expressing insecticidal toxins from bacillus thuringiensis (bt), little is known about the mechanism of resistance to these toxins. the purpose of this study is to identify genes associated with the mechanism of cry1f toxin resistance in european corn borer (ostrinia nubilalis hübner). for this, we compared the global transcriptomic response of laboratory selected resistant and susceptible o. nubilalis strain to cry1f to ... | 2015 | 26220297 |
| host-feeding pattern of aedes aegypti and aedes albopictus (diptera: culicidae) in heterogeneous landscapes of south andaman, andaman and nicobar islands, india. | mosquito foraging behavior is a determinant of host-vector contact and has an impact on the risk of arboviral epidemics. therefore, blood-feeding patterns is a useful tool for assessing the role in pathogen transmission by vector mosquitoes. competent vectors of dengue and chikungunya viz. aedes aegypti and aedes albopictus are widely prevalent in the andaman and nicobar archipelago. considering the vector potential, medical importance of both these mosquito species and lack of information on ho ... | 2015 | 26220560 |
| chemical component and proteomic study of the amphibalanus (= balanus) amphitrite shell. | as typical biofoulers, barnacles possess hard shells and cause serious biofouling problems. in this study, we analyzed the protein component of the barnacle amphibalanus (= balanus) amphitrite shell using gel-based proteomics. the results revealed 52 proteins in the a. amphitrite shell. among them, 40 proteins were categorized into 11 functional groups based on kog database, and the remaining 12 proteins were unknown. besides the known proteins in barnacle shell (sipc, carbonic anhydrase and aci ... | 2015 | 26222041 |
| weather regulates location, timing, and intensity of dengue virus transmission between humans and mosquitoes. | dengue is one of the most aggressively expanding mosquito-transmitted viruses. the human burden approaches 400 million infections annually. complex transmission dynamics pose challenges for predicting location, timing, and magnitude of risk; thus, models are needed to guide prevention strategies and policy development locally and globally. weather regulates transmission-potential via its effects on vector dynamics. an important gap in understanding risk and roadblock in model development is an e ... | 2015 | 26222979 |
| enhanced cuticular penetration as the mechanism for synergy of insecticidal constituents of rosemary essential oil in trichoplusia ni. | synergistic interactions between constituents of essential oils have been reported for several areas of research. in the present study, mechanisms that could explain the synergistic action of the two major insecticidal constituents of rosemary oil, 1,8-cineole and camphor against the cabbage looper, trichoplusia ni were investigated. 1,8-cineole was more toxic than camphor when applied topically to larvae, and when coadministered in their ratio naturally occurring in rosemary oil, the binary mix ... | 2015 | 26223769 |
| antiviral phosphorodiamidate morpholino oligomers are protective against chikungunya virus infection on cell-based and murine models. | chikungunya virus (chikv) infection in human is associated with debilitating and persistent arthralgia and arthritis. currently, there is no specific vaccine or effective antiviral available. anti-chikv phosphorodiamidate morpholino oligomer (cpmo) was evaluated for its antiviral efficacy and cytotoxcity in human cells and neonate murine model. two cpmos were designed to block translation initiation of a highly conserved sequence in chikv non-structural and structural polyprotein, respectively. ... | 2015 | 26224141 |
| two inwardly rectifying potassium channels, irk1 and irk2, play redundant roles in drosophila renal tubule function. | inwardly rectifying potassium channels play essential roles in renal physiology across phyla. barium-sensitive k(+) conductances are found on the basolateral membrane of a variety of insect malpighian (renal) tubules, including drosophila melanogaster. we found that barium decreases the lumen-positive transepithelial potential difference in isolated perfused drosophila tubules and decreases fluid secretion and transepithelial k(+) flux. in those insect species in which it has been studied, trans ... | 2015 | 26224687 |
| the asian rice gall midge (orseolia oryzae) mitogenome has evolved novel gene boundaries and tandem repeats that distinguish its biotypes. | the complete mitochondrial genome of the asian rice gall midge, orseolia oryzae (diptera; cecidomyiidae) was sequenced, annotated and analysed in the present study. the circular genome is 15,286 bp with 13 protein-coding genes, 22 trnas and 2 ribosomal rna genes, and a 578 bp non-coding control region. all protein coding genes used conventional start codons and terminated with a complete stop codon. the genome presented many unusual features: (1) rearrangement in the order of trnas as well as pr ... | 2015 | 26226163 |
| making mosquito taxonomy useful: a stable classification of tribe aedini that balances utility with current knowledge of evolutionary relationships. | the tribe aedini (family culicidae) contains approximately one-quarter of the known species of mosquitoes, including vectors of deadly or debilitating disease agents. this tribe contains the genus aedes, which is one of the three most familiar genera of mosquitoes. during the past decade, aedini has been the focus of a series of extensive morphology-based phylogenetic studies published by reinert, harbach, and kitching (rh&k). those authors created 74 new, elevated or resurrected genera from wha ... | 2015 | 26226613 |
| activation of tlr2 and tlr6 by dengue ns1 protein and its implications in the immunopathogenesis of dengue virus infection. | dengue virus (dv) infection is the most prevalent mosquito-borne viral disease and its manifestation has been shown to be contributed in part by the host immune responses. in this study, pathogen recognition receptors, toll-like receptor (tlr) 2 and tlr6 were found to be up-regulated in dv-infected human pbmc using immunofluorescence staining, flow cytometry and western blot analyses. using elisa, il-6 and tnf-α, cytokines downstream of tlr2 and tlr6 signaling pathways were also found to be up-r ... | 2015 | 26226614 |
| draft genome of the most devastating insect pest of coffee worldwide: the coffee berry borer, hypothenemus hampei. | the coffee berry borer, hypothenemus hampei, is the most economically important insect pest of coffee worldwide. we present an analysis of the draft genome of the coffee berry borer, the third genome for a coleopteran species. the genome size is ca. 163 mb with 19,222 predicted protein-coding genes. analysis was focused on genes involved in primary digestion as well as gene families involved in detoxification of plant defense molecules and insecticides, such as carboxylesterases, cytochrome p450 ... | 2015 | 26228545 |
| combination immunotherapy in the treatment of chronic bilateral panuveitis and uveitic glaucoma during acute dengue fever infection in the caribbean. | ocular manifestations of the dengue fever virus include bilateral panuveitis that can occur after the acute systemic infection has resolved. in most reported cases, the inflammation resolves with topical or systemic steroid therapy. we report a case of chronic, refractory bilateral panuveitis and uveitic glaucoma that began during the acute phase of the systemic infection and required treatment with oral steroids, multiple steroid-sparing agents, and surgical therapy for glaucoma. | 2015 | 26229512 |
| extracellular synthesis of silver nanoparticles using bacillus megaterium against malarial and dengue vector (diptera: culicidae). | biosynthesis of silver nanoparticles has provoked nowadays and alternative to physical and chemical approaches. in the present study, silver nanoparticles (agnps) were synthesized extracellular method using bacillus megaterium. the agnps formations were confirmed initially through color change, and the aliquots were characterized through uv-visible spectrophotometer, followed by scanning electron microscopy (sem), energy-dispersive x-ray (edx) spectroscopy, and fourier transform infrared (ftir) ... | 2015 | 26231835 |
| molecular insight into dengue virus pathogenesis and its implications for disease control. | dengue virus (denv) is a mosquito-transmitted rna virus that infects an estimated 390 million humans each year. here, we review recent advances in our understanding of the biology of denv and describe knowledge gaps that have impacted the development of effective vaccines and therapeutics. | 0 | 26232221 |
| [neonatal dengue. presentation of clinical cases]. | dengue is an infectious disease caused by a flavivirus, with four serotypes, transmitted by the mosquito aedes aegypti. in mexico it is a public health problem, especially in the region of central and southeast of the country. the disease can be asymptomatic or present serious forms and even death. it is confirmed by detection of the ns1 antigen; igm antibodies, polymerase chain reaction and virus isolation. the vertical transmission to de newborn has been little studied. 7 cases in neonates fro ... | 2015 | 26233977 |
| cry4ba and cyt1aa proteins from bacillus thuringiensis israelensis: interactions and toxicity mechanism against aedes aegypti. | individual crystal proteins from bacillus thuringiensis israelensis exhibit variable levels of insecticidal activities against mosquito larvae. in all cases, they are much less active compared to the whole crystal proteins due to described complex synergistic interactions among them. in the present study we investigated the effects of cyt1a98 (a cyt1aa type protein) on cry4blb (a cry4ba type toxin) insecticidal activity toward the dengue vector aedes aegypti. the bioassay analyses demonstrated t ... | 2015 | 26238170 |
| the genetic architecture of a complex trait: resistance to multiple toxins produced by bacillus thuringiensis israelensis in the dengue and yellow fever vector, the mosquito aedes aegypti. | the bacterial insecticide bacillus thuringiensis subsp. israelensis (bti) is an increasingly popular alternative to chemical insecticides for controlling mosquito populations. because bti toxicity relies on the action of four main toxins, resistance to bti is very likely a complex phenotype involving several genes simultaneously. dissecting the underlying genetic basis thus requires associating a quantitative measure of resistance to genetic variation at many loci in a segregating population. he ... | 2015 | 26238211 |
| plant-borne ovicides in the fight against mosquito vectors of medical and veterinary importance: a systematic review. | mosquitoes (diptera: culicidae) are a huge threat for millions of people worldwide, since they act as vectors for devastating parasites and pathogens. culicidae control is of crucial importance. mosquito eggs, larvae, and pupae are usually targeted using organophosphates, insect growth regulators, and microbial agents. indoor residual spraying and insecticide-treated bed nets are also employed. however, these chemicals have negative effects on human health and the environment, and induce resista ... | 2015 | 26239801 |
| association among house infestation index, dengue incidence, and sociodemographic indicators: surveillance using geographic information system. | we identified dengue transmission areas by using the geographic information systems located at local surveillance units of the itaboraí municipality in state of rio de janeiro. we considered the association among the house infestation index, the disease incidence, and sociodemographic indicators during a prominent dengue outbreak in 2007 and 2008. | 2015 | 26243266 |
| better to be in bad company than to be alone? aedes vectors respond differently to breeding site quality in the presence of others. | this study focuses on two competing species, aedes aegypti and aedes albopictus (diptera: culicidae), both invasive mosquitoes of the new world. context-specific competition between immature forms inside containers seems to be an important determinant of the coexistence or displacement of each species in different regions of the world. here, competition experiments developed at low density (one, two or three larvae) and receiving four different resource food concentration, were designed to test ... | 2015 | 26244511 |
| diversification of the genus anopheles and a neotropical clade from the late cretaceous. | the anopheles genus is a member of the culicidae family and consists of approximately 460 recognized species. the genus is composed of 7 subgenera with diverse geographical distributions. despite its huge medical importance, a consensus has not been reached on the phylogenetic relationships among anopheles subgenera. we assembled a comprehensive dataset comprising the coi, coii and 5.8s rrna genes and used maximum likelihood and bayesian inference to estimate the phylogeny and divergence times o ... | 2015 | 26244561 |
| how diverse detrital environments influence nutrient stoichiometry between males and females of the co-occurring container mosquitoes aedes albopictus, ae. aegypti, and culex quinquefasciatus. | allocation patterns of carbon and nitrogen in animals are influenced by food quality and quantity, as well as by inherent metabolic and physiological constraints within organisms. whole body stoichiometry also may vary between the sexes who differ in development rates and reproductive allocation patterns. in aquatic containers, such as tree holes and tires, detrital inputs, which vary in amounts of carbon and nitrogen, form the basis of the mosquito-dominated food web. differences in development ... | 2015 | 26244643 |
| forced egg retention and oviposition behavior of malaria, dengue and filariasis vectors to a topical repellent diethyl-phenylacetamide. | egg retention and oviposition behavior of four species of mosquito vectors viz., anopheles stephensi, aedes aegypti, aedes albopictus, and culex quinquefasciatus to a topical insect repellent diethyl-phenylacetamide (depa) at 0.1-1000 mg/l was investigated under laboratory conditions. based on oviposition activity indices, depa demonstrated concentration dependent oviposition deterrent effect to a. stephensi (-0.18 to -0.97), a. aegypti (-0.18 to -0.91) and a. albopictus (-0.50 to -0.98) females ... | 2015 | 26245028 |
| effect of chloroxylon swietenia dc bark extracts against culex quinquefasciatus, aedes aegypti, and anopheles stephensi larvae. | mosquitoes are the vector of more diseases and cause major health problems like malaria, dengue, chikungunya, and lymphatic filariasis. this article deals with the mosquito larvicidal activity of chloroxylon swietenia dc bark extracts against late third instar larvae of culex quinquefasciatus, aedes aegypti, and anopheles stephensi. methanolic crude extract of ch. swietenia bark was obtained by soxhlet apparatus and aqueous crude extract by cold percolation method. the range of concentrations of ... | 2015 | 26246308 |
| the usual suspects: comparison of the relative roles of potential urban chikungunya virus vectors in australia. | the global re-emergence of chikungunya virus (chikv) over the last decade presents a serious public health risk to australia. an increasing number of imported cases further underline the potential for local transmission to occur if local mosquitoes bite an infected traveller. laboratory experiments have identified a number of competent australian mosquito species, including the primary vectors of chikv abroad, aedes aegypti and aedes albopictus, and local endemic species aedes vigilax and aedes ... | 2015 | 26247366 |
| drosophila kash-domain protein klarsicht regulates microtubule stability and integrin receptor localization during collective cell migration. | during collective migration of the drosophila embryonic salivary gland, cells rearrange to form a tube of a distinct shape and size. here, we report a novel role for the drosophila klarsicht-anc-syne homology (kash) domain protein klarsicht (klar) in the regulation of microtubule (mt) stability and integrin receptor localization during salivary gland migration. in wild-type salivary glands, mts became progressively stabilized as gland migration progressed. in embryos specifically lacking the kas ... | 2015 | 26247519 |
| mosquitocidal and oviposition repellent activities of the extracts of seaweed bryopsis pennata on aedes aegypti and aedes albopictus. | the ever-increasing threat from infectious diseases and the development of insecticide resistance in mosquito populations drive the global search for new natural insecticides. the aims of this study were to evaluate the mosquitocidal activity of the extracts of seaweed bryopsis pennata against dengue vectors aedes aegypti and aedes albopictus, and determine the seaweed's toxic effect on brine shrimp nauplii (as a non-target organism). in addition, the chemical compositions of the active larvicid ... | 2015 | 26247928 |
| molecular cloning and functional studies of two kazal-type serine protease inhibitors specifically expressed by nasonia vitripennis venom apparatus. | two cdna sequences of kazal-type serine protease inhibitors (kspis) in nasonia vitripennis, nvkspi-1 and nvkspi-2, were characterized and their open reading frames (orfs) were 198 and 264 bp, respectively. both nvkspi-1 and nvkspi-2 contained a typical kazal-type domain. real-time quantitative pcr (rt-qpcr) results revealed that nvkspi-1 and nvkspi-2 mrnas were mostly detected specifically in the venom apparatus, while they were expressed at lower levels in the ovary and much lower levels in oth ... | 2015 | 26248077 |
| remarkable repellency of ligusticum sinense (umbelliferae), a herbal alternative against laboratory populations of anopheles minimus and aedes aegypti (diptera: culicidae). | for personal protection against mosquito bites, user-friendly natural repellents, particularly from plant origin, are considered as a potential alternative to applications currently based on synthetics such as deet, the standard chemical repellent. this study was carried out in thailand to evaluate the repellency of ligusticum sinense hexane extract (lhe) against laboratory anopheles minimus and aedes aegypti, the primary vectors of malaria and dengue fever, respectively. | 2015 | 26249666 |
| trends in genome dynamics among major orders of insects revealed through variations in protein families. | insects belong to a class that accounts for the majority of animals on earth. with over one million identified species, insects display a huge diversity and occupy extreme environments. at present, there are dozens of fully sequenced insect genomes that cover a range of habitats, social behavior and morphologies. in view of such diverse collection of genomes, revealing evolutionary trends and charting functional relationships of proteins remain challenging. | 2015 | 26251035 |
| regulation of physiological processes by micrornas in insects. | micrornas (mirnas) are small non-coding rnas that function in gene regulatory processes in plants and animals by targeting sites within messenger rna. in insects, mirnas have been shown to regulate a variety of physiological processes throughout insect development, including molting, metamorphosis, oogenesis, embryogenesis, behavior and host-pathogen interactions. the roles of mirnas in the model organism, drosophila melanogaster, have been studied extensively due to the conserved nature of mirn ... | 0 | 26251827 |
| the mysterious multi-modal repellency of deet. | deet is the most effective insect repellent available and has been widely used for more than half a century. here, i review what is known about the olfactory and contact mechanisms of deet repellency. for mosquitoes, deet has at least two molecular targets: odorant receptors (ors) mediate the effect of deet at a distance, while unknown chemoreceptors mediate repellency upon contact. additionally, the ionotropic receptor ir40a has recently been identified as a putative deet chemosensor in drosoph ... | 0 | 26252744 |
| laboratory evaluation of acute toxicity of the essential oil of allium tuberosum leaves and its selected major constituents against apolygus lucorum (hemiptera: miridae). | the aim of this research was to evaluate acute toxicity of the essential oil of leaves of chinese chives, allium tuberosum rottler ex spreng (asparagales: alliaceae) and its major constituents against apolygus lucorum meyer-dür (hemiptera: miridae). the essential oil of a. tuberosum leaves was obtained by hydrodistillation and analyzed by gas chromatography and gas chromatography-mass spectrometry. the major constituents of the oil were sulfur-containing compounds, including allyl methyl trisulf ... | 2015 | 26254289 |
| metazseckb: the human and animal secretome and subcellular proteome knowledgebase. | the subcellular location of a protein is a key factor in determining the molecular function of the protein in an organism. metazseckb is a secretome and subcellular proteome knowledgebase specifically designed for metazoan, i.e. human and animals. the protein sequence data, consisting of over 4 million entries with 121 species having a complete proteome, were retrieved from uniprotkb. protein subcellular locations including secreted and 15 other subcellular locations were assigned based on eithe ... | 2015 | 26255309 |
| 20-hydroxyecdysone stimulation of juvenile hormone biosynthesis by the mosquito corpora allata. | juvenile hormone iii (jh) is synthesized by the corpora allata (ca) and plays a key role in mosquito development and reproduction. jh titer decreases in the last instar larvae allowing pupation and metamorphosis to progress. as the anti-metamorphic role of jh comes to an end, the ca of the late pupa (or pharate adult) becomes again "competent" to synthesize jh, which plays an essential role orchestrating reproductive maturation. 20-hydroxyecdysone (20e) prepares the pupae for ecdysis, and would ... | 2015 | 26255691 |
| multiple factors contribute to anautogenous reproduction by the mosquito aedes aegypti. | aedes aegypti is an anautogenous mosquito that must blood feed on a vertebrate host to produce and lay a clutch of eggs. the rockpool mosquito, georgecraigius atropalpus, is related to a. aegypti but is a facultatively autogenous species that produces its first clutch of eggs shortly after emerging without blood feeding. consumption of a blood meal by a. aegypti triggers the release of ovary ecdysteroidogenic hormone (oeh) and insulin-like peptide 3 (ilp3) from the brain, which stimulate egg for ... | 2015 | 26255841 |
| antiviral and antioxidant activities of sulfated galactomannans from plants of caatinga biome. | dengue represents a serious social and economic public health problem; then trying to contribute to improve its control, the objective of this research was to develop phytoterapics for dengue treatment using natural resources from caatinga biome. galactomannans isolated from adenanthera pavonina l., caesalpinia ferrea mart., and dimorphandra gardneriana tull were chemically sulfated in order to evaluate the antioxidant, and antiviral activities and the role in the inhibition of virus denv-2 in v ... | 2015 | 26257815 |
| parity and longevity of aedes aegypti according to temperatures in controlled conditions and consequences on dengue transmission risks. | in guadeloupe, aedes aegypti mosquitoes are the only vectors of dengue and chikungunya viruses. for both diseases, vector control is the only tool for preventing epidemics since no vaccine or specific treatment is available. however, to efficiently implement control of mosquitoes vectors, a reliable estimation of the transmission risks is necessary. to become infective an ae. aegypti female must ingest the virus during a blood meal and will not be able to transmit the virus during another blood- ... | 2015 | 26258684 |
| atp binding cassette transporter mediates both heme and pesticide detoxification in tick midgut cells. | in ticks, the digestion of blood occurs intracellularly and proteolytic digestion of hemoglobin takes place in a dedicated type of lysosome, the digest vesicle, followed by transfer of the heme moiety of hemoglobin to a specialized organelle that accumulates large heme aggregates, called hemosomes. in the present work, we studied the uptake of fluorescent metalloporphyrins, used as heme analogs, and amitraz, one of the most regularly used acaricides to control cattle tick infestations, by rhipic ... | 2015 | 26258982 |
| engaging scientists: an online survey exploring the experience of innovative biotechnological approaches to controlling vector-borne diseases. | pioneering technologies (e.g., nanotechnology, synthetic biology or climate engineering) are often associated with potential new risks and uncertainties that can become sources of controversy. the communication of information during their development and open exchanges between stakeholders is generally considered a key issue in their acceptance. while the attitudes of the public to novel technologies have been widely considered there has been relatively little investigation of the perceptions an ... | 2015 | 26259589 |
| effect of ultraviolet-a radiation on the production of leptolegnia chapmanii (saprolegniales: saprolegniaceae) zoospores on dead aedes aegypti (diptera: culicidae) larvae and their larvicidal activity. | impact of uv-radiation in entomopathogens in aquatic environments remains little investigated. the present study reports on the effect of uv-a on the larvicidal activity of leptolegnia chapmanii zoospores in aedes aegypti; on the production of zoospores in larvae killed by the pathogen and then exposed to uv-a; and on the activity of these zoospores against healthy larvae. whereas the virulence of free zoospores in a. aegypti larvae was affected by a uv-a exposure time longer than 10min, product ... | 2015 | 26259676 |
| diversity and abundance of mosquitoes (diptera:culicidae) in an urban park: larval habitats and temporal variation. | urban parks are areas designated for human recreation but also serve as shelter and refuge for populations of several species of native fauna, both migratory and introduced. in brazil, the effect of annual climate variations on aedes aegypti and dengue epidemics in large cities like são paulo is well known, but little is known about how such variations can affect the diversity of mosquito vectors in urban parks and the risk of disease transmission by these vectors. this study investigates the in ... | 2015 | 26259817 |
| the essential role of vitellogenin receptor in ovary development and vitellogenin uptake in bactrocera dorsalis (hendel). | the vitellogenin receptor (vgr) functions as an essential component in uptaking and transporting vitellogenin (vg) in female adults, which is involved in ovary development and oviposition. this study aimed to clarify the molecular characteristics and function of vgr in the oriental fruit fly bactrocera dorsalis (hendel). here, we identified the full-length of bdvgr (genbank accession no. jx469118), encoding a 1925 residue (aa) protein with a 214.72 kda molecular mass and several typical motifs o ... | 2015 | 26262609 |
| partial characterization of venom from the colombian spider phoneutria boliviensis (aranae:ctenidae). | we report on the first studies on the characterization of venom from phoneutria boliviensis (aranae:ctenidae) (f. o. pickard-cambridge, 1897), done with colombian species. after the electrostimulation extraction process, the venom showed physicochemical properties corresponding to a colorless and water-soluble liquid with a density of 0.86 mg/ml and 87% aqueous content. p. boliviensis venom and rp-hplc fractions showed hemolytic activity and hydrolyzed the synthetic substrate 4-nitro-3-octanoylo ... | 2015 | 26264023 |
| mosquito larvicidal and pupaecidal potential of prodigiosin from serratia marcescens and understanding its mechanism of action. | mosquitoes spread lethal diseases like malaria and dengue fever to humans. considering mosquito vector control as one of the best alternatives to reduce new infections, here we have analyzed the effect of purified pigment prodigiosin extracted from serratia marcescens (nmcc 75) against larval and pupal stages of aedes aegypti and anopheles stephensi mosquitoes. mosquito larvicidal activities of purified prodigiosin revealed lc50 values of 14 ± 1.2, 15.6 ± 1.48, 18 ± 1.3, 21 ± 0.87 µg/ml against ... | 2015 | 26267052 |
| expression of dengue virus ns3 protein in drosophila alters its susceptibility to infection. | we developed a drosophila model in which the dengue virus ns3 protein is expressed in a tissue specific and inducible manner. dengue virus ns3 is a multifunctional protein playing a major role during viral replication. both protease and helicase domains of ns3 are interacting with human and insect host proteins including innate immune components of the host machinery. we characterized the ns3 transgenic flies showing that ns3 expression did not affect fly development. to further study the links ... | 2015 | 26267447 |
| practices of dengue fever prevention and the associated factors among the orang asli in peninsular malaysia. | dengue is prevalent among malaysia's indigenous peoples, known as the orang asli, and it poses a serious health threat to them. the study aims to look at the socio-demographic factors, health beliefs, and knowledge about dengue and its association to dengue prevention practices among orang asli communities in peninsular malaysia. | 2015 | 26267905 |
| assessment of the impact of potential tetracycline exposure on the phenotype of aedes aegypti ox513a: implications for field use. | aedes aegypti is the primary vector of dengue fever, a viral disease which has an estimated incidence of 390 million infections annually. conventional vector control methods have been unable to curb the transmission of the disease. we have previously reported a novel method of vector control using a tetracycline repressible self-limiting strain of ae. aegypti ox513a which has achieved >90% suppression of wild populations. | 2015 | 26270533 |
| candidate biomarkers for mosquito age-grading identified by label-free quantitative analysis of protein expression in aedes albopictus females. | we applied a "shotgun" approach based on nanoliquid chromatography-high resolution mass spectrometry associated to label free quantification (lfq) to identify proteins varying with age, independently from the physiological state, in aedes albopictus, a mosquito species which in the last decades invaded temperate regions in north america and europe, creating concerns for associated high nuisance and risk of arbovirus transmission. the combined "shotgun" and lfq approach was shown to be highly sui ... | 2015 | 26271156 |
| antiviral defense mechanisms in honey bees. | honey bees are significant pollinators of agricultural crops and other important plant species. high annual losses of honey bee colonies in north america and in some parts of europe have profound ecological and economic implications. colony losses have been attributed to multiple factors including rna viruses, thus understanding bee antiviral defense mechanisms may result in the development of strategies that mitigate colony losses. honey bee antiviral defense mechanisms include rna-interference ... | 0 | 26273564 |
| acute toxicity and dermal and eye irritation of the aqueous and hydroalcoholic extracts of the seeds of "zapote" pouteria mammosa (l.) cronquist. | the common use of pouteria mammosa (l.) cronquist, "mamey or zapote," in food and ethnobotanic medicine shows its low or absent toxicity as fruit extracts prepared from seeds. however, it is essential to conduct security trials to scientifically support their use in drug therapy. this study evaluated the aqueous and hydroalcoholic extract (25%) acute oral toxicity, obtained from the seeds of p. mammosa, in sprague dawley rats and dermal and eye irritability in new zealand rabbits. the 404 and 40 ... | 2015 | 26273696 |
| spatial and temporal clustering of chikungunya virus transmission in dominica. | using geo-referenced case data, we present spatial and spatio-temporal cluster analyses of the early spread of the 2013-2015 chikungunya virus (chikv) in dominica, an island in the caribbean. spatial coordinates of the locations of the first 417 reported cases observed between december 15th, 2013 and march 11th, 2014, were captured using the global positioning system (gps). we observed a preponderance of female cases, which has been reported for chikv outbreaks in other regions. we also noted st ... | 2015 | 26274813 |