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[entomological evaluation of the risk of urban outbreak of yellow fever in 2008 in abidjan, côte d'ivoire].in april 2008, seven years after the outbreak of 2001, abidjan faced another epidemic of yellow fever. three weeks after the case detected in the commune of treichville at 28(th) of april 2008, two other cases were notified, the first one in port-bouet and the second one in cocody located respectively in the south and north of the city of abidjan. in order to determine a large-scale risk of urban yellow fever epidemic, epidemiological and entomological investigations were conducted by the minist ...201623693032
development and evaluation of a pyriproxyfen-treated device to control the dengue vector, aedes aegypti (l.) (diptera:culicidae).the resurgence of dengue fever and the chikungunya epidemic make the control of aedes aegypti mosquitoes, the vectors of these diseases, critically important. we developed and evaluated an ae. aegypti control device that is visually-attractive to mosquitoes. this pyriproxyfen-treated device was evaluated for its impact on ae. aegypti egg production and population dynamics in dengue-endemic areas in thailand. the device consists of a "high rise" shaped ovitrap/ resting station covered with black ...201323691625
relationship between rainfall and aedes larval population at two insular sites in pulau ketam, selangor, malaysia.two insular settlements (kampung pulau ketam and kampung sungai lima) were selected to study the population dynamics of aedes aegypti and aedes albopictus mosquitoes, vectors of dengue and chikungunya infections. ovitrap surveillance was conducted between october 2007 and october 2008. there was an inverse negative association between ovitrap index and rainfall at the time of collection, probably because rainfall increased the number of available oviposition sites. rainfall and ovitrap index wer ...201323691624
mosquitocidal properties of basella rubra and cleome viscosa against aedes aegypti (linn.) (diptera:culicidae).to investigate the larvicidal and ovicidal activities of acetone, benzene, hexane and methanol leaf extracts of basella rubra and cleome viscosa against dengue vector, aedes aegypti.201323690200
temporal and spatial distribution of dengue vector mosquitoes and their habitat patterns in penang island, malaysia.we studied the diversity of aedes breeding sites in various urban, suburban, and rural areas over time between february 2009 and february 2010 in the dengue endemic areas of penang island, malaysia. we categorized the breeding sites and efficiency, and identified the key breeding containers. among the 3 areas, the rural areas produced the highest container index (55), followed by suburban (42) and urban (32) areas. the numbers of key premises and containers were significantly higher (p < 0.000) ...201323687853
gene flow pattern among aedes aegypti populations in mexico.patterns of gene flow vary greatly among aedes aegypti populations throughout mexico. the populations are panmictic along the pacific coast, isolated by distance in northeast mexico, and exhibit moderate gene flow across the yucatan peninsula. nine ae. aegypti collections from 6 cities in oaxaca, mexico, were taken to examine the local patterns of gene flow. genetic variation was examined in a 387-bp region of the nicotinamide adenine dinucleotide dehydrogenase subunit 4 mitochondrial gene (nd4) ...201323687850
[characterization of bacillus thuringrensis var. israelesis larvicide granules to larvae of mosquitoes].to develop a new formulation of bacillus thuringrensis var. israelesis (bti) granules and assess its effect on mosquito control.201323687813
chemometric studies on potential larvicidal compounds against aedes aegypti.the mosquito aedes aegypti (diptera, culicidae) is the vector of yellow and dengue fever. in this study, chemometric tools, such as, principal component analysis (pca), consensus pca (cpca), and partial least squares regression (pls), were applied to a set of fifty five active compounds against ae. aegypti larvae, which includes terpenes, cyclic alcohols, phenolic compounds, and their synthetic derivatives. the calculations were performed using the volsurf+ program. cpca analysis suggests that t ...201423676010
mosquito larvicidal and ovicidal activity of puffer fish extracts against anopheles stephensi, culex quinquefasciatus and aedes aegypti (diptera: culicidae).the extracts of liver (le), ovary (oe), skin (se) and muscle (me) tissues of four species of puffer fishes viz., arothron hispidus, lagocephalus inermis, lagocephalus scleratus and chelonodon patoca were evaluated against larvae and eggs of three mosquito vectors, anopheles stephensi, culex quinquefasciatus and aedes aegypti. the lc50 values were 1194.26, 1382.73 (le); 1421.42, 1982.73 (oe); 7116.86, 15038.98 (me) and 10817.8 ppm (se) for an. stephensi and cx. quinquefasciatus respectively for a ...201323665705
aedes aegypti disregard humidity-related conditions with adequate nutrition.weather variations have clear associations with the epidemiology of dengue fever and populations of aedes aegypti mosquitoes. data on humidity associations, however, lags with respect to its effect on host-biting, nectar-seeking and survival. this experimental study on ae. aegypti, sourced from the arid tropics, investigated the effect of low and high relative humidity and diet in relation to host-biting, temporal variations in feeding frequency, and mosquito mortality. in each environmental set ...201323665702
population structure of the mosquito aedes aegypti (stegomyia aegypti) in pakistan.eleven microsatellite markers were used to determine the genetic population structure and spread of aedes aegypti (stegomyia aegypti) (diptera: culicidae) in pakistan using mosquitoes collected from 13 different cities. there is a single genetic cluster of ae. aegypti in pakistan with a pattern of isolation by distance within the population. the low level of isolation by distance suggests the long-range passive dispersal of this mosquito, which may be facilitated by the tyre trade in pakistan. a ...201323662926
residual and pre-treatment application of starycide insect growth regulator (triflumuron) to control aedes aegypti in containers.dengue is not endemic in australia, although the vector mosquito aedes aegypti is. in order to prevent dengue establishment, australia maintains tight control of vector mosquitoes. tight control programs often necessitate regular repeat application of treatments, and this is resource intensive. we sought to investigate the efficacy and longevity of triflurumon starycide® insect growth regulator under field conditions in tropical australia against aedes. we compared two doses (0.48 and 0.96 ppm), ...201423653423
larvicidal effect of aqueous and ethanolic extracts of senna alata on anopheles gambiae, culex quinquefasciatus and aedes aegypti.senna alata is locally used in south eastern nigeria in the treatment of several infections which include ringworm and other parasitic skin diseases.the larvicidal activities of aqueous and ethanolic leaf and stem extracts of s. alata were evaluated in static bioassays, on fourth instar larvae of anopheles gambiae, culex quinquefasciatus and aedes aegypti, at extract concentrations of 0.15, 0.30, 0.45, 0.60 and 0.75% w/v, for 72 hours. mortality of larvae exposed to the different extracts increa ...201323625430
spatiotemporal patterns of aedes aegypti populations in cairns, australia: assessing drivers of dengue transmission.to identify the meteorological drivers of dengue vector density and determine high- and low-risk transmission zones for dengue prevention and control in cairns, australia.201323617766
modelling aedes aegypti mosquito control via transgenic and sterile insect techniques: endemics and emerging outbreaks.the invasion of pest insects often changes or destroys a native ecosystem, and can result in food shortages and disease endemics. issues such as the environmental effects of chemical control methods, the economic burden of maintaining control strategies and the risk of pest resistance still remain, and mosquito-borne diseases such as malaria and dengue fever prevail in many countries, infecting over 100 million worldwide in 2010. one environmentally friendly method for mosquito control is the st ...201323608633
implications of saline concentrations for the performance and competitive interactions of the mosquitoes aedes aegypti (stegomyia aegypti) and aedes albopictus (stegomyia albopictus).aedes albopictus (stegomyia albopictus) (diptera: culicidae) has probably supplanted aedes aegypti (stegomyia aegypti) throughout most of its historical range in the u.s.a., although ae. aegypti still exists in large coastal cities in southern florida. we measured salt concentrations in field containers along an axis perpendicular to the coast and examined intraspecific outcomes in these species under different salt concentrations in a factorial study using varying intra- and interspecific densi ...201423607885
larvicidal activity against aedes aegypti of pacharin from bauhinia acuruana.the aim of the present study was to evaluate the activity of pacharin isolated from the ethanol extract from roots of bauhinia acuruana on third-instar larvae of aedes aegypti linn. (diptera: culicidae). the crude ethanol extract showed larvicidal activity at the concentration of 500 μg/ml. given this larvicidal activity, this extract was submitted to chromatographic fractionation on a silica gel column eluted with n-hexane, dichloromethane, ethyl ether, ethyl acetate, and methanol in order to i ...201323604564
public health significance of invasive mosquitoes in europe.there are currently five invasive aedes mosquito species known to be established in europe, namely aedes albopictus, aedes aegypti, aedes japonicus, aedes atropalpus and aedes koreicus. aedes albopictus and aedes aegypti are the incriminated vectors in the recent outbreaks of chikungunya and dengue fever in europe. however, both laboratory experiments and field observations indicate that these invasive mosquitoes have a potential to also transmit other pathogens of public health importance. incr ...201323574618
insecticide resistance in two aedes aegypti (diptera: culicidae) strains from costa rica.dengue (family flaviridae, genus flavivirus, denv) and dengue hemorrhagic fever (dhf) are presently important public health problems in costa rica. the primary strategy for disease control is based on reducing population densities of the main mosquito vector aedes aegypti (l.) (diptera: culicidae). this is heavily dependent on use of chemical insecticides, thus the development of resistance is a frequent threat to control program effectiveness. the objective of this study was to determine the le ...201323540124
larvicidal and cytotoxic activities of extracts from 11 native plants from northeastern mexico.of all mosquito-borne viral diseases, dengue is spreading most rapidly worldwide. conventional chemical insecticides (e.g., organophosphates and carbamates) effectively kill mosquitoes at their larval stage, but are toxic to humans. natural product-based insecticides may be highly specific. herein, we report the insecticidal activities of 11 native mexican plants against aedes aegypti (l). ether extracts of ambrosia confertiflora de candolle, thymus vulgaris (l.), and zanthoxylum fagara (l.), an ...201323540118
a dengue vector surveillance by human population-stratified ovitrap survey for aedes (diptera: culicidae) adult and egg collections in high dengue-risk areas of taiwan.aedes aegypti l. is the primary dengue vector in southern taiwan. this article is the first report on a large-scale surveillance program to study the spatial-temporal distribution of the local ae. aegytpi population using ovitraps stratified according to the human population in high dengue-risk areas. the sampling program was conducted for 1 yr and was based on weekly collections of eggs and adults in kaohsiung city. in total, 10,380 ovitraps were placed in 5,190 households. paired ovitraps, one ...201323540112
hot temperatures can force delayed mosquito outbreaks via sequential changes in aedes aegypti demographic parameters in autocorrelated environments.aedes aegypti l. (diptera: culicidae) is a common pantropical urban mosquito, vector of dengue, yellow fever and chikungunya viruses. studies have shown ae. aegypti abundance to be associated with environmental fluctuations, revealing patterns such as the occurrence of delayed mosquito outbreaks, i.e., sudden extraordinary increases in mosquito abundance following transient extreme high temperatures. here, we use a two-stage (larvae and adults) matrix model to propose a mechanism for environment ...201423537497
larvicidal activities and chemical composition of essential oils from piper klotzschianum (kunth) c. dc. (piperaceae).volatile oils from fresh roots, stems, leaves and seeds of piper klotzschianum (piperaceae) were obtained by hydrodistillation and analysed by gc-fid and gc-ms. in total, 25 components, representing more than 95% of the examined oils, were identified. the essential oils were evaluated against artemia salina leach nauplii and fourth-instar aedes aegypti larvae.201323526766
calcitonin receptor 1 (aedaegpcrcal1) hindgut expression and direct role in myotropic action in females of the mosquito aedes aegypti (l.).in anautogenous mosquitoes such as aedes aegypti females the calcitonin-like diuretic hormone 31 (dh₃₁) stimulates natriuretic fluid excretion from the malpighian tubules (mts) after a blood meal. we previously cloned and functionally characterized aedaegpcrcal1 from a. aegypti, the ortholog of the drosophila melanogaster dh₃₁ receptor and immunolocalized it in the mts. however, localization of the calcitonin receptor-like receptor 1 (gpcrcal1) in the hindgut of any insect is unknown, and specif ...201323523607
synthesis and biological activity of substituted urea and thiourea derivatives containing 1,2,4-triazole moieties.a series of novel thiourea and urea derivatives containing 1,2,4-triazole moieties were synthesized and evaluated for their antifungal and larvicidal activity. triazole derivatives 3a-e and 4a-e were synthesized by reacting thiocarbohydrazide with thiourea and urea compounds 1a-e and 2a-e, respectively, in a 130-140 °c oil bath. the proposed structures of all the synthesized compounds were confirmed using elemental analysis, uv, ir, 1h-nmr and mass spectroscopy. all compounds were evaluated for ...201323519199
diffusion of community empowerment strategies for aedes aegypti control in cuba: a muddling through experience.effective participatory strategies in dengue control have been developed and assessed as small-scale efforts. the challenge is to scale-up and institutionalize these strategies within dengue control programs. we describe and critically analyze the diffusion process of an effective empowerment strategy within the cuban aedes aegypti control program, focusing on decision-making at the national level, to identify ways forward to institutionalize such strategies in cuba and elsewhere. from 2005 to 2 ...201323517703
[identification and spatial distribution of key premises for aedes aegypti in the porto dantas neighborhood, aracaju, sergipe state, brazil, 2007-2008].currently the best way to avoid new dengue epidemics is to control the mosquito vector aedes aegypti. this study thus aimed to identify and analyze the spatial distribution of "key premises" for ae. aegypti in aracaju, sergipe state, brazil. three entomological surveys were performed prior to, during, and after the dengue epidemic and in different conditions of precipitation: the end of the rainy season, beginning of the rainy season, and the dry season. key premises were identified by positivit ...201323459822
[segregation of aedes aegypti and culex quinquefasciatus (diptera: culicidae) niche under laboratory conditions].aedes and culex are two mosquito genuses of epidemiological importance, being aedes aegypti and culex quinquefasciatus the predominant ones in urban areas. it is common to find both of them associated in the same breeding sites where they share a lot of things despite the literature.201323444638
[residual effectiveness of temephos observed in a mexican southeast city affected by dengue].to study the effect of water extraction and renewal on the residual action of temephos applied at 1ppm to cement water-storage containers at home.201323444636
further insecticidal activities of essential oils from lippia sidoides and croton species against aedes aegypti l.this study assessed new insecticidal activities of essential oils from lippia sidoides and croton species (croton zehntneri, croton nepetaefolius, croton argyrophylloides, and croton sonderianus) against aedes aegypti mosquito. in addition, the acute toxicity upon mice was determined. all essential oils showed inhibition of egg hatching, with ic50 values ranging from 66.4 to 143.2 μg ml(-1), larvicidal activity with lc50 ranging from 25.5 to 94.6 μg ml(-1), and pupicidal action with pc50 ranging ...201323435925
comparison of aedes aegypti (diptera: culicidae) resting behavior on two fabric types under consideration for insecticide treatment in a push-pull strategy.aedes aegypti (l.), the primary vector of dengue and dengue hemorrhagic fever, breeds and rests predominately inside human dwellings. with no current vaccine available, vector control remains the mainstay for dengue management and novel approaches continue to be needed to reduce virus transmission. this requires a full understanding of ae. aegypti ecology to design effective strategies. one novel approach is the use of contact irritants at target resting sites inside homes to make the surface un ...201323427653
[k-othrine wg250 (deltamethrine)-based treatment in an area characterized by high infestation rates of aedes aegypti].dengue and its lethal form, that is, dengue hemorrhagic fever, has significantly increased in las americas, being the aedes aegypti the main and the only transmitting vector till now.201323424809
[predatory capacity of macrobrachium tenellum on aedes aegypti larvae in lab conditions].in the last few years, a lot of importance has been given to natural predators against aedes aegypti. several organisms have been studied both in lab and in the field so as to find out their capacity to devour mosquito larvae. high densities of macrobrachium tenellum are found in natural conditions, it is not aggressive and may stand wide ranges of temperature, rates of salinity and oxygen concentrations.201523424807
[impact of an educational intervention carried out in anti-vector campaign workers].aedes aegypti is a common vector in cuba, which damages human health. santiago de cuba is one of the provinces with high number of mosquito foci in a sustained way. therefore, it is very important, from the social viewpoint, to carry out educational interventions with anti-vector campaign workers in mella municipality in this province.201323424806
[partial characterization of esterase activity in a temephos-resistant aedes aegypti strain].the esterase enzymes have been defined as the mechanism of resistance to temephos in aeges aegypti in cuba, which is the most used larvacide worldwide.201323424802
insecticidal and biting deterrent activity of rose-scented geranium (pelargonium spp.) essential oils and individual compounds against stephanitis pyrioides and aedes aegypti.rose-scented geranium, pelargonium spp., essential oils from the cultivars 'bourbon', 'china', 'egypt', 'rober's lemon rose' and 'frensham' were analyzed by gc-fid and gc-ms. a total of 136 compounds were identified from five essential oils, constituting 85.5-99.7% of the oils. essential oils and pure compounds were evaluated for their insecticidal activity against stephanitis pyrioides and larvicidal and biting deterrent activity against aedes aegypti.201323423995
larval rearing temperature influences the effect of malathion on aedes aegypti life history traits and immune responses.the effects of anthropogenic chemical contaminants on aquatic organisms are largely influenced by underlying environmental conditions. this study evaluated how larval rearing temperature influences the impact of malathion on the fitness of the yellow fever mosquito aedes aegypti. larvae were exposed to water control, and low (0.03mg/l) or high (0.05mg/l) malathion dose at 20°c, 25°c and 30°c and emergence rate, time to emergence, female fecundity and expression of genes encoding two antimicrobia ...201323419321
field-collected permethrin-resistant aedes aegypti from central thailand contain point mutations in the domain iis6 of the sodium channel gene (kdr).one of the mechanisms responsible for pyrethroid resistance in mosquitoes is mutations in domain iis6 of voltage-gated sodium channel gene (kdr). aedes aegypti larvae were collected from the central provinces of thailand (bangkok, prachin buri and ratchaburi) and colonized until they became adults. partial fragment of kdr of permethrin-resistant mosquitoes were amplified by rt-pcr and sequenced. among the four nucleotide mutations detected, two mutations resulted in two amino acid substitutions, ...201223413701
the key breeding sites by pupal survey for dengue mosquito vectors, aedes aegypti (linnaeus) and aedes albopictus (skuse), in guba, cebu city, philippines.we conducted this study to assess how well a pupal survey of dengue mosquito vectors, aedes aegypti and aedes albopictus, is able to target the most productive breeding sites. the study was carried out monthly during the rainy season (8 months) in 2008 in cuba, cebu city, philippines. the hypotheses tested were: 1) most pupae of ae. aegypti or ae. albopictus were produced in a few types of breeding sites and 2) the most productive types of breeding sites for each species were the most abundant. ...201223413699
gustatory receptor neuron responds to deet and other insect repellents in the yellow-fever mosquito, aedes aegypti.three gustatory receptor neurons were characterized for contact chemoreceptive sensilla on the labella of female yellow-fever mosquitoes, aedes aegypti. the neuron with the smallest amplitude spike responded to the feeding deterrent, quinine, as well as n,n-diethyl-3-methylbenzamide and other insect repellents. two other neurons with differing spikes responded to salt (nacl) and sucrose. this is the first report of a gustatory receptor neuron specific for insect repellents in mosquitoes and may ...201323407786
the component of carica papaya seed toxic to a. aegypti and the identification of tegupain, the enzyme that generates it.as aedes aegypti transmits the etiologic agents of both yellow and dengue fever; vector control is considered essential to minimise their incidence. the aim of this work was to identify the component of carica papaya seed toxic to a. aegypti, and the identification of tegupain, the enzyme that generates it. aqueous extracts (1%, w/v) of the seed tegument and cotyledon of c. papaya are not larvicidal isolately. however, a mixture of 17μgml(-1) tegument extract and 27μgml(-1) cotyledon extract cau ...201323402920
evolutionary history of eukaryotic α-glucosidases from the α-amylase family.although some α-glucosidases from the α-amylase family (glycoside hydrolase family gh13) have been studied extensively, their exact number, organization on the chromosome, and orthology/paralogy relationship were unknown. this was true even for important disease vectors where gut α-glucosidase is known to be receptor for the bin toxin used to control the population of some mosquito species. in some cases orthologs from related species were studied intensively, while potentially important paralog ...201323397242
studies on effects of andrographis paniculata (burm.f.) and andrographis lineata nees (family: acanthaceae) extracts against two mosquitoes culex quinquefasciatus (say.) and aedes aegypti (linn.).to investigate the studies on effects of andrographis paniculata (a. paniculata) (burm.f.) and andrographis lineata (a. lineata) nees (family: acanthaceae) extracts against two mosquitoes culex quinquefasciatus (cx. quinquefasciatus) (say.) and aedes aegypti (ae. aegypti) (linn.).201323375029
growth-disrupting, larvicidal and neurobehavioral toxicity effects of seed extract of seseli diffusum against aedes aegypti (l.) (diptera: culicidae).plant extracts that kill, deform the post-embryonic molting stages of mosquitoes could be a valuable approach in integrated vector management programmes to replace synthetic chemical pesticides, containing persistent toxic substances. crude ethanolic seed extract was obtained by maceration of seeds of indian celery, seseli diffusum (apiaceae). the ethanolic seed extract induced strong neurobehavioral toxicity against the 4th instar larvae of aedes aegypti which resulted in a high level of larval ...201323357563
green synthesis of silver nanoparticles using murraya koenigii leaf extract against anopheles stephensi and aedes aegypti.mosquitoes transmit serious human diseases, causing millions of deaths every year. the use of synthetic insecticides to control vector mosquitoes has caused physiological resistance and adverse environmental effects in addition to high operational cost. insecticides of synthesized natural products for vector control have been a priority in this area. in the present study, the activity of silver nanoparticles (agnps) synthesized using murraya koenigii plant leaf extract against first to fourth in ...201323322327
the efficacy of a combined larvicide-adulticide in ultralow volume and fumigant canister formulations in controlling the dengue vector aedes aegypti (diptera: culicidae) in northwest of argentina.the efficacy of an ultralow volume formulation (ulv) and fumigant canister, containing both permethrin and pyriproxyfen, was compared with that of standard permethrin applications in a field assay conducted in banda del río salí, tucumán (north-western argentina). five treatment areas were established: first area was sprayed with a ulv formulation of 10 % permethrin, a second area was treated using a fumigant canister containing 10 % permethrin and 3 % pyriproxyfen, the third area was sprayed wi ...201323322325
larvicidal isoxazoles: synthesis and their effective susceptibility towards aedes aegypti larvae.twenty 3,5-disubstituted isoxazoles have been synthesized and tested against fourth instar aedes aegypti larvae. in the synthesis of title compounds, modifications have been made in the c-5 side-chain with a view to test their larvicidal activity. these isoxazoles have been obtained by 1,3-dipolar cycloaddition of arylnitrile oxides to terminal alkynes which furnished the desired products in 20% to 79% yields. a comparative study of the larvicidal activity between 3-(3-aryl-isoxazol-5-yl)-propan ...201323321014
mosquito attractant blends to trap host seeking aedes aegypti.aedes aegypti is the key vector of three important arboviral diseases -dengue, yellow fever and chikungunya. to identify volatile chemicals which could be used in odour based traps for aedes mosquito surveillance, a few synthetic compounds and compound blends have been evaluated in an indigenously designed olfactometer. a total of 24 compounds and seven compound blends were screened against unfed adult female ae. aegypti mosquitoes for attraction and compared with control group. the attractancy ...201323306388
dengue vector control and surveillance during a major outbreak in a coastal red sea area in sudan.an unprecedented dengue outbreak occurred in 2010 in port sudan city, sudan. dengue incidence was 94 cases per 10 000 observed over 17 epidemiological weeks (total cases = 3 765). we report here the impact of the vector control response plan to the outbreak, which mainly entailed house inspection and insecticide space spraying. in total 3 048 houses were inspected during vector surveillance and 19 794 larvae and 3 240 pupae of aedes aegypti were collected. entomological indices decreased during ...201223301396
spatial and temporal patterns of abundance of aedes aegypti l. (stegomyia aegypti) and aedes albopictus (skuse) [stegomyia albopictus (skuse)] in southern florida.invasion by mosquito vectors of disease may impact the distribution of resident mosquitoes, resulting in novel patterns of vectors and concomitant risk for disease. one example of such an impact is the invasion by aedes albopictus (skuse) [stegomyia albopictus (skuse)] (diptera: culicidae) of north america and this species' interaction with aedes aegypti l. (stegomyia aegypti l). we hypothesized that ae. aegypti would be found in urban, coastal areas that experience hotter and drier conditions, ...201323278304
ovicidal, repellent, adulticidal and field evaluations of plant extract against dengue, malaria and filarial vectors.mosquitoes are insect vectors responsible for the transmission of parasitic and viral infections to millions of people worldwide, with substantial morbidity and mortality. infections transmitted by mosquitoes include malaria, yellow fever, chikungunya, filariasis and other arboviruses. insecticides of botanical origin may serve as suitable alternative biocontrol techniques in the future. the adulticidal activities of crude hexane, benzene, ethyl acetate, acetone and methanol leaf extracts of aca ...201323271569
synergistic actions of formamidine insecticides on the activity of pyrethroids and neonicotinoids against aedes aegypti (diptera: culicidae).formamidines are unique insecticides and acaricides that elicit multiple effects in controlling insects. here, we tested two formamidines, amitraz, and chlordimeform, for their synergistic actions on type ii pyrethroids and neonicotinoids to increase their larvicidal actions on the fourth instars of aedes aegypti l. an organophosphate insecticide was used as a negative control. after 24 h, the synergism of formamidines was highest on imidacloprid, followed by two type ii pyrethroids, deltamethri ...201223270169
laboratory evaluation techniques to investigate the spatial potential of repellents for push and pull mosquito control systems.a protocol has been developed for the indoor evaluation of candidate spatial repellents intended for use in push and pull systems. single treatments (catnip oil, 1-methylpiperazine, and homopiperazine) and a mixture of catnip oil and homopiperazine were tested with yellow-fever mosquitoes (aedes aegypti) in y-tube olfactometers to determine 1) if these compounds inhibited mosquito host-seeking at short distances and 2) if results obtained in olfactometer tests can be correlated with a larger sca ...201223270167
aedes aegypti (diptera: culicidae) biting deterrence: structure-activity relationship of saturated and unsaturated fatty acids.in this study we evaluated the biting deterrent effects of a series of saturated and unsaturated fatty acids against aedes aegypti (l), yellow fever mosquito (diptera: culicidae) using the k & dbioassay module system. saturated (c6:0 to c16:0 and c18:0) and unsaturated fatty acids (c11:1 to c14:1, c16:1, c18:1, and c18:2) showed biting deterrence index (bdi) values significantly greater than ethanol, the negative control. among the saturated fatty acids, mid chain length acids (c10:0 to c13:0) s ...201223270165
aedes (ochlerotatus) epactius along an elevation and climate gradient in veracruz and puebla states, méxico.we report on the collection ofimmatures of aedes (ochlerotatus) epactius dyar & knab from artificial containers during july through september 2011 in 12 communities located along an elevation and climate gradient extending from sea level in veracruz state to high elevations (>2,000 m) in veracruz and puebla states, méxico. ae. epactius was collected from 11 of the 12 study communities; the lone exception was the highest elevation community along the transect (>2,400 m). this mosquito species was ...201223270151
integration of botanical and bacterial insecticide against aedes aegypti and anopheles stephensi.the present study evaluated the orthosiphon thymiflorus leaf extract and the bacterial insecticide spinosad, testing the first to fourth instars larvae and pupae of two important vector mosquitoes, viz., aedes aegypti, anopheles stephensi. the fresh leaves of o. thymiflorus were washed thoroughly in tap water and shade-dried at room temperature (28 ± 2 °c) for 5 to 8 days. the air-dried materials were powdered separately using a commercial electrical blender. from the plants, 500 g powder was ma ...201323242266
ongoing outbreak of dengue type 1 in the autonomous region of madeira, portugal: preliminary report.following the identification of two autochthonous cases of dengue type 1 on 3 october 2012, an outbreak of dengue fever has been reported in madeira, portugal. as of 25 november, 1,891 cases have been detected on the island where the vector aedes aegypti had been established in some areas since 2005. this event represents the first epidemic of dengue fever in europe since 1928 and concerted control measures have been initiated by local health authorities.201223231893
[evaluation of effectiveness of several repellents against mosquito bites available at the polish market].background. mosquitoes are blood-sucking insects, nuisance to humans and animals. their bites cause itching and allergic reactions. these insects are also vectors of several viruses, bacteria and parasites. protection against mosquitoes is therefore justified and desirable. this can give repellents and products for protection small outdoor areas such as terraces, home gardens. objective. the aim of this study was to evaluate the effectiveness of eight selected products with different formulation ...201223230720
discovery of antagonists of tick dopamine receptors via chemical library screening and comparative pharmacological analyses.ticks transmit a wide variety of disease causing pathogens to humans and animals. considering the global health impact of tick-borne diseases, there is a pressing need to develop new methods for vector control. we are exploring arthropod dopamine receptors as novel targets for insecticide/acaricide development because of their integral roles in neurobiology. herein, we developed a screening assay for dopamine receptor antagonists to further characterize the pharmacological properties of the two ...201223213654
efficacy of six neonicotinoid insecticides alone and in combination with deltamethrin and piperonyl butoxide against pyrethroid-resistant aedes aegypti and anopheles gambiae (diptera: culicidae).in this study, carried out on pyrethroid-resistant aedes aegypti and anopheles gambiae, different combinations containing pyrethroid deltamethrin, piperonyl butoxide (pbo) synergist and six neonicotinoid insecticides (acetamiprid, nitenpyram, thiamethoxam, clothianidin, imidacloprid and thiacloprid) were tested. the bioassays were carried out by tarsal contact on impregnated paper on ae. aegypti and with impregnated pieces of netting on an. gambiae.201323208775
ecological interactions in aedes species on reunion island.two invasive, container-breeding mosquito species, aedes aegypti (stegomyia aegypti) and aedes albopictus (stegomyia albopicta) (diptera: culicidae), have different distribution patterns on reunion island. aedes albopictus occurs in all areas and ae. aegypti colonizes only some restricted areas already occupied by ae. albopictus. this study investigates the abiotic and biotic ecological mechanisms that determine the distribution of aedes species on reunion island. life history traits (duration o ...201323205822
productivity and population density estimates of the dengue vector mosquito aedes aegypti (stegomyia aegypti) in australia.new mosquito control strategies centred on the modifying of populations require knowledge of existing population densities at release sites and an understanding of breeding site ecology. using a quantitative pupal survey method, we investigated production of the dengue vector aedes aegypti (l.) (stegomyia aegypti) (diptera: culicidae) in cairns, queensland, australia, and found that garden accoutrements represented the most common container type. deliberately placed 'sentinel' containers were se ...201323205694
bdelloid rotifer, philodina species in the breeding containers of aedes aegypti and aedes albopictus.the vector mosquitoes of dengue and chikungunya fever, aedes aegypti and aedes albopictus have adapted to feed on humans and undergo larval and pupal development in natural and artificial freshwater collections. although several studies reported, still, much information is required to understand the successful survival of aedes mosquitoes in small temporary containers. in an investigation conducted in the chikungunya affected areas of kerala state, india, the presence of bdelloid rotifer, philod ...201223202612
oral receptivity of aedes aegypti from cape verde for yellow fever, dengue, and chikungunya viruses.at the end of 2009, 21,313 cases of dengue-3 virus (denv-3) were reported in the islands of cape verde, an archipelago located in the atlantic ocean 570 km from the coast of western africa. it was the first dengue outbreak ever reported in cape verde. mosquitoes collected in july 2010 in the city of praia, on the island of santiago, were identified morphologically as aedes aegypti formosus. using experimental oral infections, we found that this vector showed a moderate ability to transmit the ep ...201323199267
protein kinase a-dependent and -independent activation of the v-atpase in malpighian tubules of aedes aegypti.transepithelial ion transport in insect malpighian tubules is energized by an apical v-atpase. in hematophagous insects, a blood meal during which the animal ingests huge amounts of salt and water stimulates transepithelial transport processes linked to v-atpase activation, but how this is accomplished is still unclear. here we report that membrane-permeant derivatives of camp increase the bafilomycin-sensitive atpase activity in malpighian tubules of aedes aegypti twofold and activate atp-depen ...201323197085
pre-imaginal development of aedes aegypti in brackish and fresh water urban domestic wells in sri lanka. 201223181875
the influence of local environment on the aging and mortality of aedes aegypti (l.): case study in fortaleza-ce, brazil.it is generally assumed that the daily probability of survival of mosquitoes is independent of age. to test this assumption we have conducted a three-year experimental fieldwork study (2005-2007) at fortaleza-ce in brazil, determining daily survival rates of the dengue vector aedes aegypti (l.). survival rates of adult ae. aegypti may be age-dependent and the statistical analysis is a sensitive approach for comparing patterns of mosquito survival. the mosquito survival data were better fit by a ...201223181868
synergistic effect of eugenia jambolana linn. and solidago canadensis linn. leaf extracts with deltamethrin against the dengue vector aedes aegypti linn. at mysore.with the goal in mind to minimize the application of environmentally hazardous chemical insecticides, the larvicidal activity of two plant extracts along with deltamethrin was studied at university of mysore. the extracts of solidago canadensis and eugenia jambolana were employed for working out the synergistic efficacy against aedes aegypti larvae, as the extracts of both the plants exhibited high efficacy when applied individually. the deltamethrin when analyzed separately, lc50 and lc90 value ...201323179216
evaluation of matrix-assisted laser desorption/ionization time of flight mass spectrometry for the identification of ceratopogonid and culicid larvae.matrix-assisted laser desorption/ionization time of flight mass spectrometry (maldi-tof ms) was evaluated for the rapid identification of ceratopogonid larvae. optimal sample preparation as evaluated with laboratory-reared biting midges culicoides nubeculosus was the homogenization of gut-less larvae in 10% formic acid, and analysis of 0.2 mg/ml crude protein homogenate mixed with sa matrix at a ratio of 1:1.5. using 5 larvae each of 4 ceratopogonid species (c. nubeculosus, c. obsoletus, c. deco ...201323171762
chemical constituents and larvicidal potential of feronia limonia leaf essential oil against anopheles stephensi, aedes aegypti and culex quinquefasciatus.in the present investigation, the leaf essential oil of feronia limonia was evaluated for chemical constituents and mosquito larvicidal activity against the larvae of anopheles stephensi, aedes aegypti and culex quinquefasciatus. gc and gc-ms analyses revealed that the essential oil contain 51 compounds. estragole (34.69 %) and β-pinene(23.59 %) were identified as the major constituents followed by methyl (z)-caryophyllene (11.05 %), eugenol (6.50 %), linalool (3.97 %), phytol (3.27 %), sabinene ...201323160893
larvicidal activity against aedes aegypti of essential oils from northeast brazil.the larvicidal activities of essential oils from the leaves of artemisia vulgaris l., cymbopogon flexuosus (nees ex steud.) wats. and piper tuberculatum jacq. were evaluated using third-instar larvae of aedes aegypti. the essential oils were obtained by hydrodistillation and analyzed by gc and gc-ms. the essential oil of p. tuberculqatum had the lowest lc50 value (106.3 +/- 2.2 microg/ml), followed by that of a. vulgaris (114.1 +/- 1.7 microg/ml) and c. flexuosus (121.6 +/- 0.8 micro/ml). the re ...201223157019
larvicidal activity of isoflavonoids from muellera frutescens extracts against aedes aegypti.the biological activity of extracts from the leaves, bark and roots of muellera frutescens, an amazonian ichtyotoxic plant, were evaluated to find new environmentally safe insecticides. the n-hexane extracts of bark, leaf, and root showed a strong toxic activity against aedes aegypti mosquito larvae. bioguided fractionation of the bark extract led to the isolation of seven isoflavonoids (12a-hydroxyelliptone, elliptone, (-)-variabilin, rotenone, rotenolone, tephrosin and deguelin). rotenone and ...201223156998
fitness evaluation of two brazilian aedes aegypti field populations with distinct levels of resistance to the organophosphate temephos.in brazil, decades of dengue vector control using organophosphates and pyrethroids have led to dissemination of resistance. although these insecticides have been employed for decades against aedes aegypti in the country, knowledge of the impact of temephos resistance on vector viability is limited. we evaluated several fitness parameters in two brazilian ae. aegypti populations, both classified as deltamethrin resistant but with distinct resistant ratios (rr) for temephos. the insecticide-suscep ...201223147149
four additional hepatozoon species (apicomplexa: hepatozoidae) from north florida ratsnakes, genus pantherophis.records from a colubrid host are reported for hepatozoon horridus, described originally from a viperid snake. hepatozoon horridus in pantherophis guttatus (colubridae) has gamonts 14-18.0 by 4.0-5.5 microm, with length by width (lw) 60-99 microm2, and l/w ratio 2.5-3.9. spherical to elongate, usually ovoid oocysts with l/w ratio 1.0-3.7 contain 16-160 spherical to usually ovoid sporocysts 15-31 by 14-26 microm, with l/w ratio 1.0-1.4, that contain 5-24 sporozoites. two additional hepatozoon spec ...201223136796
genetically modified mosquito: the malaysian public engagement experience.on december 21, 2010, 6000 genetically modified (gm) mosquitoes were released in an uninhabited forest in malaysia. the purpose of the deliberate release was a limited “marked release and recapture” (mrr) experiment, a standard ecological method in entomology, to evaluate under field conditions, the flight distance and longevity of the sterile male aedes aegypti strain ox513a(my1), a gm strain. as with any other gm technologies, the release was received with mixed responses. as the scientific co ...201223125042
biting deterrence and insecticidal activity of hydrazide-hydrazones and their corresponding 3-acetyl-2,5-disubstituted-2,3-dihydro-1,3,4-oxadiazoles against aedes aegypti.taking into account the improvement in insecticidal activity by the inclusion of fluorine in the hydrazone moiety, the authors synthesized new 4-fluorobenzoic acid hydrazides and 3-acetyl-2,5-disubstituted-2,3-dihydro-1,3,4-oxadiazoles, substituting a phenyl group or a heteroaryl ring carrying one or two atoms of f, cl and br, and investigated their biting deterrent and larvicidal activities against aedes aegypti for the first time.201323117879
synergistic larvicidal effect and morphological alterations induced by ethanolic extracts of annona muricata and piper nigrum against the dengue fever vector aedes aegypti.phytopesticide combinations of different botanical sources are seldom reported. annona muricata seed and piper nigrum fruit ethanolic extracts enriched in acetogenins and piperamides, respectively, were synergistically used as larvicides against the dengue fever vector aedes aegypti.201323112098
household disposables as breeding habitats of dengue vectors: linking wastes and public health.an assessment of the household wastes as larval habitats of the dengue vectors was made considering kolkata, india, as geographical area. wastes of four major categories, namely, earthen, porcelain, plastic and coconut shells were monitored for positive with immature of either aedes aegypti or aedes albopictus. twenty six types of wastes with varying size and shape, resembling containers, were identified that hosted mosquito immature. the number of waste containers positive for aedes immature va ...201323107350
[aedes aegypti l. and aedes albopictus skuse mosquitoes are a new biological threat to the south of russia]. 201423088153
insecticide susceptibility status of field-collected aedes (stegomyia) aegypti (l.) at a dengue endemic site in shah alam, selangor, malaysia.biweekly ovitrap surveillance (os) was conducted for a year (august 2007 - september 2008) at two different dengue endemic sites in shah alam, selangor, malaysia, 50 km from kuala lumpur. aedes aegypti collected from these 2 locations were raised to the f3 stage and subjected to a who standard bioassay method to determine lethal time (lt) against pyrethroids (permethrin 0.75%, cyfluthrin 0.15%), organophosphates (malathion 5.0%, fenitrothion 1.0%), carbamates (propoxur 0.1%, bendiocarb 0.1%) and ...201223082552
proof of concept for a novel insecticide bioassay based on sugar feeding by adult aedes aegypti (stegomyia aegypti).aedes aegypti l. (stegomyia aegypti) (diptera: culicidae) is the principal vector of dengue and yellow fever viruses in tropical and subtropical regions of the world. disease management is largely based on mosquito control achieved by insecticides applied to interior resting surfaces and through space sprays. population monitoring to detect insecticide resistance is a significant component of integrated disease management programmes. we developed a bioassay method for assessing insecticide susce ...201323077986
dengue-2 virus carrying capacity of thai aedes aegypti strains with different susceptibility to deltamethrin.deltamethrin-resistant aedes aegypti currently threatens the effectiveness of dengue hemorrhagic fever control operations in thailand. although a previous study has suggested that insecticide resistance may increase ae. aegypti susceptibility to dengue-2 virus infection, our experimental data showed no significant association between laboratory-induced deltamethrin-resistance in a thai ae. aegypti isolate and its susceptibility to dengue -2 infection.201223077842
an eco-physiological model of the impact of temperature on aedes aegypti life history traits.physiological processes mediate the impact of ecological conditions on the life histories of insect vectors. for the dengue/chikungunya mosquito, aedes aegypti, three life history traits that are critical to urban population dynamics and control are: size, development rate and starvation mortality. in this paper we make use of prior laboratory experiments on each of these traits at 2°c intervals between 20 and 30°c, in conjunction with eco-evolutionary theory and studies on a.aegypti physiology, ...201223068992
molecular mechanisms associated with increased tolerance to the neonicotinoid insecticide imidacloprid in the dengue vector aedes aegypti.mosquitoes are vectors of several major human diseases and their control is mainly based on the use of chemical insecticides. resistance of mosquitoes to organochlorines, organophosphates, carbamates and pyrethroids led to a regain of interest for the use of neonicotinoid insecticides in vector control. the present study investigated the molecular basis of neonicotinoid resistance in the mosquito aedes aegypti. a strain susceptible to insecticides was selected at the larval stage with imidaclopr ...201323058251
biosynthesized silver nanoparticles from pedilanthus tithymaloides leaf extract with anti-developmental activity against larval instars of aedes aegypti l. (diptera; culicidae).mosquitoes transmit dreadful diseases to human beings wherein biological control of these vectors using plant-derived molecules would be an alternative to reduce mosquito population. aqueous leaf extract and green synthesized silver nanoparticles (ag nps) from pedilanthus tithymaloides (l.) poit. were investigated for their efficacy against the dengue vector aedes aegypti l. (diptera; culicidae). the biologically synthesized ag nps were characterized by uv-vis spectrum, x-ray diffraction, fourie ...201323052770
laboratory observations on the larvicidal efficacy of three plant species against mosquito vectors of malaria, dengue/dengue hemorrhagic fever (df/dhf) and lymphatic filariasis in the semi-arid desert.comparative larvicidal efficacy of aqueous and organic solvent extracts from seeds, leaves and flowers of three desert plants viz. calotropis procera (aiton), tephrosia purpurea (l.) pers. and prosopis juliflora (sw.) dc. was evaluated against anopheles stephensi (liston), aedes aegypti (linnaeus) and culex quinquefasciatus (say). for this purpose larvae of all the three mosquito species were reared in the laboratory and studies carried out on late 3rd or early 4th instars using standard who tec ...201223029912
challenges in the control of aedes aegypti. 201223011453
field evaluations of disposable sticky lures for surveillance of aedes aegypti (stegomyia aegypti) and culex quinquefasciatus in jakarta.from december 1997 to april 1998, disposable sticky lures (1608 lure days) were trialled in homes in north jakarta, indonesia as surveillance tools for aedes aegypti (stegomyia aegypti) (diptera: culicidae) and culex quinquefasciatus (diptera: culicidae), referenced to indoor resting adult collections (92 × 10 min). the lures collected 89.4% of the total of 1339 ae. aegypti and 92.1% of the total of 1272 cx. quinquefasciatus collected by all methods. because there were no significant differences ...201323002913
morphological and morphometrical assessment of spermathecae of aedes aegypti females.the vectorial capacity of aedes aegypti is directly influenced by its high reproductive output. nevertheless, females are restricted to a single mating event, sufficient to acquire enough sperm to fertilize a lifetime supply of eggs. how ae. aegypti is able to maintain viable spermatozoa remains a mystery. male spermatozoa are stored within either of two spermathecae that in ae. aegypti consist of one large and two smaller organs each. in addition, each organ is divided into reservoir, duct and ...201222990957
adulticidal properties of cardiospermum halicacabum plant extracts against three important vector mosquitoes.to determine adulticidal activitiy of hexane, ethyl acetate, benzene, chloroform and methanol leaf extracts of cardiospermum halicacabum against culex quinquefasciatus (cx. quinquefasciatus), aedes aegypti (ae. aegypti) and anopheles stephensi (an. stephensi).201222957423
insecticide susceptibility status in three medically important species of mosquitoes, anopheles stephensi, aedes aegypti and culex quinquefasciatus, from bruhat bengaluru mahanagara palike, karnataka, india.development of resistance among mosquito vectors of diseases is of growing concern in many countries of the subtropics, including much of india. the distributions of susceptibility/resistance to insecticides are scarcely recorded and are unknown in many parts of india. this manuscript reports the susceptibility status among strains of anopheles stephensi, aedes aegypti and culex quinquefasciatus collected from different geographical areas of bruhat bengaluru mahanagara palike (bbmp), karnataka, ...201322926921
impact of environmental temperatures on resistance to organophosphate insecticides in aedes aegypti from trinidad.to examine the effects of increasing larval rearing temperatures on the resistance status of trinidadian populations of aedes aegypti to organophosphate (op) insecticides.201222910718
field comparison of thermal and non-thermal ultra-low-volume applications using water and diesel as solvents for managing dengue vector, aedes aegypti.to compare the effectiveness on aedes aegypti (linneo) (diptera: culicidae) of a larvicide-adulticide ulv formulation applied by a thermal or a cold fogger using different solvents.201222906100
effect of ageratum houstonianum mill. (asteraceae) leaf extracts on the oviposition activity of anopheles stephensi, aedes aegypti and culex quinquefasciatus (diptera: culicidae).plant extracts have been studied extensively for their insecticidal activity against immature stages and adult mosquitoes. they have also been reported to influence the habitat preference of ovipositing mosquitoes. ageratum houstonianum, a medicinal plant belonging to the family asteraceae, has been reported to possess insecticidal activity, and in the present study, the ovipositional attractant/deterrent activity was studied. the effect of ageratum houstonianum crude leaf extracts on the ovipos ...201222903449
mosquito larvicidal, pupicidal, adulticidal, and repellent activity of artemisia nilagirica (family: compositae) against anopheles stephensi and aedes aegypti.mosquito-borne diseases have an economic impact, including loss in commercial and labor outputs, particularly in countries with tropical and subtropical climates; however, no part of the world is free from vector-borne diseases. the aim of the present study, to evaluate the larvicidal, pupicidal, repellent, and adulticidal activities of methanol crude extract of artemisia nilagirica were assayed for their toxicity against two important vector mosquitoes, viz., anopheles stephensi and aedes aegyp ...201222903417
larvicidal potential of mimusops elengi against aedes aegypti (l) and culex quinquefasciatus (say). 201222898484
toxicity and synergic repellency of plant essential oil mixtures with vanillin against aedes aegypti (diptera: culicidae).in all triplicate tests of six plant essential oils and of vanillin mixtures, we corroborated strong insecticidal and repellent activities against adult aedes aegypti (l.). essential oils with potent toxic fumigant activities also exhibited repellency. compared with n, n-diethyl-3-methylbenzamide, 5% of the essential oil concentrations of cassia, lemongrass, lemon eucalyptus, and xanthoxylum oils did not show repellent effects. however, a composition oflemongrass oil, xanthoxylum oil, and vanill ...201222897048
temperature-mediated differential expression of immune and stress-related genes in aedes aegypti larvae.the mechanisms by which natural populations of vector mosquitoes cope with daily and seasonal fluctuations in temperature are poorly understood. we examined the effect of water temperature on expression of stress and immune-related genes in aedes aegypti larvae. aedes aegypti 3rd instars were exposed for 24 h to one of 7 constant temperatures (10 degrees c, 15 degrees c, 20 degrees c, 25 degrees c [control], 32 degrees c, 36 degrees c, or 40 degrees c) and expression of antimicrobial peptides (c ...201222894117
statement of retraction: "larvicidal activity of 4-hydroxycoumarin derivatives against aedes aegypti". pharmaceutical biology 2011 49:2, 190-3. 201222881145
[a school program for dengue control in honduras: from knowledge to action].the environmental school program (pea, for its spanish acronym), a dengue control initiative focused on primary schools that took place during 2005-2010 in several cities in honduras, is described. the environmental health program was designed to increase knowledge and develop skills in the identification and control of aedes aegypti breeding sites, as well as in water and solid waste management. the results, as measured by behavioral change and reduced larval indices, were satisfactory in the m ...201222858820
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