Publications

improving dengue virus capture rates in humans and vectors in kamphaeng phet province, thailand, using an enhanced spatiotemporal surveillance strategy.	dengue is of public health importance in tropical and sub-tropical regions. dengue virus (denv) transmission dynamics was studied in kamphaeng phet province, thailand, using an enhanced spatiotemporal surveillance of 93 hospitalized subjects with confirmed dengue (initiates) and associated cluster individuals (associates) with entomologic sampling. a total of 438 associates were enrolled from 208 houses with household members with a history of fever, located within a 200-m radius of an initiate ...	2015	25986580
synergistic action of octopamine receptor agonists on the activity of selected novel insecticides for control of dengue vector aedes aegypti (diptera: culicidae) mosquito.	studying insecticide resistance in mosquitoes has attracted the attention of many scientists to elucidate the pathways of resistance development and to design novel strategies in order to prevent or minimize the spread and evolution of resistance. here, we tested the synergistic action of piperonyl butoxide (pbo) and two octopamine receptor (or) agonists, amitraz (amz) and chlordimeform (cdm) on selected novel insecticides to increase their lethal action on the fourth instar larvae of aedes aegy ...	2015	25987220
unique biochemical and molecular biological mechanism of synergistic actions of formamidine compounds on selected pyrethroid and neonicotinoid insecticides on the fourth instar larvae of aedes aegypti (diptera: culicidae).	we recently reported that formamidine pesticides such as amitraz and chlordimeform effectively synergize toxic actions of certain pyrethroid and neonicotinoid insecticides in some insect species on the 4th instar larvae of aedes aegypti. here we studied the biochemical basis of the synergistic actions of the formamidines in amplifying the toxicity of neonicotinoids and pyrethroids such as dinotefuran and thiamethoxam, as well as deltamethrin-fenvalerate type of pyrethroids. we tested the hypothe ...	2015	25987221
the dengue vaccine pipeline: implications for the future of dengue control.	dengue has become the most rapidly expanding mosquito-borne infectious disease on the planet, surpassing malaria and infecting at least 390 million people per year. there is no effective treatment for dengue illness other than supportive care, especially for severe cases. symptoms can be mild or life-threatening as in dengue hemorrhagic fever and dengue shock syndrome. vector control has been only partially successful in decreasing dengue transmission. the potential use of safe and effective tet ...	2015	25989449
basophil activation by mosquito extracts in patients with hypersensitivity to mosquito bites.	hypersensitivity to mosquito bites (hmb) is a cutaneous disorder belonging to the group of epstein-barr virus (ebv)-associated t/natural killer (nk)-cell lymphoproliferative diseases, and is primarily mediated by ebv-infected nk cells. it is characterized by intense local skin reactions accompanied by general symptoms after mosquito bites, and infiltration of ebv-infected nk cells into the bite sites. however, the mechanisms underlying these reactions have not been fully examined. we recently de ...	2015	25990049
weather variability associated with aedes (stegomyia) aegypti (dengue vector) oviposition dynamics in northwestern argentina.	this study aims to develop a forecasting model by assessing the weather variability associated with seasonal fluctuation of aedes aegypti oviposition dynamic at a city level in orán, in northwestern argentina. oviposition dynamics were assessed by weekly monitoring of 90 ovitraps in the urban area during 2005-2007. correlations were performed between the number of eggs collected weekly and weather variables (rainfall, photoperiod, vapor pressure of water, temperature, and relative humidity) with ...	2015	25993415
chikungunya virus transmission potential by local aedes mosquitoes in the americas and europe.	chikungunya virus (chikv), mainly transmitted in urban areas by the mosquitoes aedes aegypti and aedes albopictus, constitutes a major public health problem. in late 2013, chikv emerged on saint-martin island in the caribbean and spread throughout the region reaching more than 40 countries. thus far, ae. aegypti mosquitoes have been implicated as the sole vector in the outbreaks, leading to the hypothesis that chikv spread could be limited only to regions where this mosquito species is dominant.	2015	25993633
mosquitocidal properties of igg targeting the glutamate-gated chloride channel in three mosquito disease vectors (diptera: culicidae).	the glutamate-gated chloride channel (glucl) is a highly sensitive insecticide target of the avermectin class of insecticides. as an alternative to using chemical insecticides to kill mosquitoes, we tested the effects of purified immunoglobulin g (igg) targeting the extracellular domain of glucl from anopheles gambiae (agglucl) on the survivorship of three key mosquito disease vectors: anopheles gambiae s.s., aedes aegypti and culex tarsalis. when administered through a single blood meal, anti-a ...	0	25994632
caribbean and la réunion chikungunya virus isolates differ in their capacity to induce proinflammatory th1 and nk cell responses and acute joint pathology.	chikungunya virus (chikv) is a mosquito-borne arthralgic alphavirus that has garnered international attention as an important emerging pathogen since 2005. more recently, it invaded the caribbean islands and the western hemisphere. intriguingly, the current chikv outbreak in the caribbean is caused by the asian chikv genotype, which differs from the la réunion lr2006 opy1 isolate belonging to the indian ocean lineage. here, we adopted a systematic and comparative approach against lr2006 opy1 to ...	2015	25995257
dengue and hepatitis e virus infection in pregnant women in eastern sudan, a challenge for diagnosis in an endemic area.	dengue fever and hepatitis e virus infection are both a public health problem in developing countries due to poor sanitation. infection with viral hepatitis and dengue fever can present with similar clinical such and fever, headache and abortion. this study was conducted in port-sudan city in the eastern part of the country. elisa and real time pcr tests were used to detect the infection. a total number of 39 pregnant women with a mean age 26 ±7.8 were included in the study. all of them had feve ...	2014	25995787
separation of binding protein of celangulin v from the midgut of mythimna separata walker by affinity chromatography.	celangulin v, an insecticidal compound isolated from the root bark of chinese bittersweet, can affect the digestive system of insects. however, the mechanism of how celangulin v induces a series of symptoms is still unknown. in this study, affinity chromatography was conducted through coupling of celangulin v-6-aminoacetic acid ester to the cnbr-activated sepharose 4b. sds-page was used to analyze the collected fraction eluted by celangulin v. eight binding proteins (zinc finger protein, thiored ...	2015	25996604
arterial hypertension and skin allergy are risk factors for progression from dengue to dengue hemorrhagic fever: a case control study.	currently, knowledge does not allow early prediction of which cases of dengue fever (df) will progress to dengue hemorrhagic fever (dhf), to allow early intervention to prevent progression or to limit severity. the objective of this study is to investigate the hypothesis that some specific comorbidities increase the likelihood of a df case progressing to dhf.	2015	25996882
agency, access, and anopheles: neighborhood health perceptions and the implications for community health interventions in accra, ghana.	social and environmental factors are increasingly recognized for their ability to influence health outcomes at both individual and neighborhood scales in the developing urban world. yet issues of spatial heterogeneity in these complex environments may obscure unique elements of neighborhood life that may be protective or harmful to human health. resident perceptions of neighborhood effects on health may help to fill gaps in our interpretation of household survey results and better inform how to ...	2015	25997424
sex determination. a male-determining factor in the mosquito aedes aegypti.	sex determination in the mosquito aedes aegypti is governed by a dominant male-determining factor (m factor) located within a y chromosome-like region called the m locus. here, we show that an m-locus gene, nix, functions as an m factor in a. aegypti. nix exhibits persistent m linkage and early embryonic expression, two characteristics required of an m factor. nix knockout with clustered regularly interspaced short palindromic repeats (crispr)-cas9 resulted in largely feminized genetic males and ...	2015	25999371
draft genome sequence of chromobacterium vaccinii, a potential biocontrol agent against mosquito (aedes aegypti) larvae.	chromobacterium vaccinii has been isolated only from cranberry bogs in massachusetts. while it is unknown what role these bacteria play in their natural environments, they hold potential as biological control agents against the larvae of insect pests. potential virulence genes were identified, including the violacein synthesis pathway, siderophores, and chitinases.	2015	25999572
dengue: moving from current standard of care to state-of-the-art treatment.	treatment of dengue remains supportive in the absence of targeted antiviral therapy or approved vaccines. responsive fluid management is key to preventing progression to shock or other severe manifestations. the dynamic natural history of dengue infection and its influence on hemodynamic homeostasis needs to be carefully considered in the planning of individualized therapy. though largely self-limiting, the sheer burden of dengue disease on the global population will result in atypical manifesta ...	0	25999799
pathways of amino acid degradation in nilaparvata lugens (stål) with special reference to lysine-ketoglutarate reductase/saccharopine dehydrogenase (lkr/sdh).	nilaparvata lugens harbors yeast-like symbionts (ylss). in present paper, a genome-wide analysis found 115 genes from ni. lugens and 90 genes from ylss that were involved in the metabolic degradation of 20 proteinogenic amino acids. these 205 genes encoded for 77 enzymes. accordingly, the degradation pathways for the 20 amino acids were manually constructed. it is postulated that ni. lugens can independently degrade fourteen amino acids (threonine, alanine, glycine, serine, aspartate, asparagine ...	2015	26000452
asymmetric mating interference between two related mosquito species: aedes (stegomyia) albopictus and aedes (stegomyia) cretinus.	aedes (stegomyia) albopictus (skuse) and aedes (stegomyia) cretinus edwards are closely related mosquito species with common morphological features and bio-ecological similarities. recent mosquito surveillance in athens, greece, showed that they are sympatric mosquito species, with ae. albopictus [corrected] developing quite higher population densities than ae. cretinus [corrected] . the potential of mating interference between these species was investigated by reciprocal and homologous mating e ...	2015	26001099
high throughput profiling of the cotton bollworm helicoverpa armigera immunotranscriptome during the fungal and bacterial infections.	innate immunity is essential in defending against invading pathogens in invertebrates. the cotton bollworm, helicoverpa armigera (hübner) is one of the most destructive lepidopteran pests, which causes enormous economic losses in agricultural production worldwide. the components of the immune system are largely unknown in this insect. the application of entomopathogens is considered as an alternative to the chemical insecticides for its control. however, few studies have focused on the molecular ...	2015	26001831
annona muricata leaf extract-mediated silver nanoparticles synthesis and its larvicidal potential against dengue, malaria and filariasis vector.	mosquitoes transmit several diseases which cause millions of deaths every year. the use of synthetic insecticides to control mosquitoes caused diverse effects to the environment, mammals, and high manufacturing cost. the present study was aimed to test the larvicidal activity of green synthesized silver nanoparticles using annona muricata plant leaf extract against third instar larvae of three medically important mosquitoes, i.e., aedes aegypti, anopheles stephensi, and culex quinquefasciatus. t ...	2015	26002825
sweetgum: an ancient source of beneficial compounds with modern benefits.	sweetgum trees are large, deciduous trees found in asia and north america. sweetgum trees are important resources for medicinal and other beneficial compounds. many of the medicinal properties of sweetgum are derived from the resinous sap that exudes when the outer bark of the tree has been damaged. the sap, known as storax, has been used for centuries to treat common ailments such as skin problems, coughs, and ulcers. more recently, storax has proven to be a strong antimicrobial agent even agai ...	2015	26009686
echinacea purpurea: pharmacology, phytochemistry and analysis methods.	echinacea purpurea (asteraceae) is a perennial medicinal herb with important immunostimulatory and anti-inflammatory properties, especially the alleviation of cold symptoms. the plant also attracted scientists' attention to assess other aspects of its beneficial effects. for instance, antianxiety, antidepression, cytotoxicity, and antimutagenicity as induced by the plant have been revealed in various studies. the findings of the clinical trials are controversial in terms of side effects. while s ...	2015	26009695
plant essential oils synergize and antagonize toxicity of different conventional insecticides against myzus persicae (hemiptera: aphididae).	plant-derived products can play an important role in pest management programs. essential oils from lavandula angustifolia (lavender) and thymus vulgaris (thyme) and their main constituents, linalool and thymol, respectively, were evaluated for insecticidal activity and synergistic action in combination with insecticides against green peach aphid, myzus persicae (sulzer) (hemiptera: aphididae). the essential oils and their main constituents exerted similar insecticidal activity when aphids were e ...	2015	26010088
accuracy of clinical diagnosis of dengue episodes in the rv144 hiv vaccine efficacy trial in thailand.	rv144 was a community-based hiv vaccine efficacy trial conducted in hiv-uninfected adults in thailand, where dengue virus continues to cause a large number of infections every year. we attempted to document the accuracy of clinically diagnosed dengue episodes reported as serious adverse events (saes) and adverse events (aes) and examine whether dengue serology would support the clinical diagnosis. subjects without a clinical dengue diagnosis but with an infection or idiopathic fever were selecte ...	2015	26011728
prospective cohort study with active surveillance for fever in four dengue endemic countries in latin america.	to prepare for a phase iii dengue vaccine efficacy trial, 20 investigational sites were selected for this observational study to identify dengue infections in a closed cohort (n = 3,000 children 9-16 years of age). of 255 acute febrile episodes experienced by 235 children, 50 (21.3%) were considered serologically probable dengue, and 18 (7.7%) were considered virologically confirmed (i.e., dengue ns1 antigen positive) dengue cases. considering the disease-free and at-risk period from study start ...	2015	26013373
the baculovirus antiapoptotic p35 protein functions as an inhibitor of the host rna interference antiviral response.	rna interference (rnai) is considered an ancient antiviral defense in diverse organisms, including insects. virus infections generate double-strand rnas (dsrnas) that trigger the rnai machinery to process dsrnas into virus-derived short interfering rnas (vsirnas), which target virus genomes, mrnas, or replication intermediates. viruses, in turn, have evolved viral suppressors of rnai (vsrs) to counter host antiviral rnai. following recent discoveries that insects mount an rnai response against d ...	2015	26018163
[omics of vector mosquitoes: a big data platform for vector biology and vector-borne diseases].	recently the studies on mosquito genomics, transcriptomics and small rnaomics developed rapidly with the novel biotechnologies of the next generation sequencing techniques. the genome sequences of several important vector mosquitoes including anopheles gambiae, culex quinquefasciatus, and aedes aegypti have been published. the genome sizes vary among the different species of mosquitoes and are consistent with the number of the repeat regions. the released genome sequences facilitate gene cloning ...	2015	26018253
dengue in an area of the colombian caribbean.	in colombia, dengue is an endemic disease and the four serotypes have been reported.	2015	26019378
blood serum and bsa, but neither red blood cells nor hemoglobin can support vitellogenesis and egg production in the dengue vector aedes aegypti.	aedes aegypti is the major vector of dengue, yellow fever and chikungunya viruses that put millions of people in endemic countries at risk. mass rearing of this mosquito is crucial for strategies that use modified insects to reduce vector populations and transmission of pathogens, such as sterile insect technique or population replacement. a major problem for vector mosquito mass rearing is the requirement of vertebrate blood for egg production since it poses significant costs as well as potenti ...	2015	26020000
predicting unprecedented dengue outbreak using imported cases and climatic factors in guangzhou, 2014.	dengue is endemic in more than 100 countries, mainly in tropical and subtropical regions, and the incidence has increased 30-fold in the past 50 years. the situation of dengue in china has become more and more severe, with an unprecedented dengue outbreak hitting south china in 2014. building a dengue early warning system is therefore urgent and necessary for timely and effective response.	2015	26020627
dengue awareness in latin american populations: a questionnaire study.	dengue is an escalating public health concern in latin american countries with a dramatic increase of cases reported during the past decade. the objectives of this study were to identify and provide insights into current management and attitudes toward dengue and to understand attitudes to vaccination and current behaviors to prevent dengue in mexico and colombia.	2015	26021614
expression of active trypsin-like serine peptidases in the midgut of sugar-feeding female anopheles aquasalis.	anopheles aquasalis is a dipteran of the family culicidae that is widely distributed in the coastal regions of south and central america. this species acts as a vector of plasmodium vivax, an important etiological agent of malaria, which represents a serious public health problem. in mosquitoes, trypsin-like serine proteases are important in blood meal digestion, immune responses and reproductive functions. the study of peptidases expressed in the mosquito midgut is essential to understanding th ...	2015	26021986
small-scale evaluation of the efficacy and residual activity of alpha-cypermethrin wg (250 g ai/kg) for indoor spraying in comparison with alpha-cypermethrin wp (50 g ai/kg) in india.	indoor residual spraying (irs) with different formulations of insecticides is being used for the control of mosquito vectors in many countries. in the present study, residual efficacy and duration of effectiveness of irs with alpha-cypermethrin wg-sb (250 g ai/m2) formulation was compared with wp formulation (50 g ai/kg) in a small scale (phase ii) field trial.	2015	26022085
the evolutionary diversity of insect retinal mosaics: common design principles and emerging molecular logic.	independent evolution has resulted in a vast diversity of eyes. despite the lack of a common bauplan or ancestral structure, similar developmental strategies are used. for instance, different classes of photoreceptor cells (prs) are distributed stochastically and/or localized in different regions of the retina. here, we focus on recent progress made towards understanding the molecular principles behind patterning retinal mosaics of insects, one of the most diverse groups of animals adapted to li ...	2015	26025917
mosquito attraction: crucial role of carbon dioxide in formulation of a five-component blend of human-derived volatiles.	behavioral responses of the malaria mosquito anopheles coluzzii (an. gambiae sensu stricto molecular 'm form') to an expanded blend of human-derived volatiles were assessed in a dual-port olfactometer. a previously documented attractive three-component blend consisting of nh3, (s)-lactic acid, and tetradecanoic acid served as the basis for expansion. adding 4.5% co2 to the basic blend significantly enhanced its attractiveness. expansion of the blend with four human-derived c4-volatiles was then ...	2015	26026743
spatial models for prediction and early warning of aedes aegypti proliferation from data on climate change and variability in cuba.	climate variability, the primary expression of climate change, is one of the most important environmental problems affecting human health, particularly vector-borne diseases. despite research efforts worldwide, there are few studies addressing the use of information on climate variability for prevention and early warning of vector-borne infectious diseases.	2015	26027583
the case for a 'one health' approach to combating vector-borne diseases.		2015	26027713
differential expression of midgut proteins in trypanosoma brucei gambiense-stimulated vs. non-stimulated glossina palpalis gambiensis flies.	the unicellular pathogenic protozoan trypanosoma brucei gambiense is responsible for the chronic form of sleeping sickness. this vector-borne disease is transmitted to humans by the tsetse fly of the group glossina palpalis, including the subspecies g. p. gambiensis, in which the parasite completes its developmental cycle. sleeping sickness control strategies can therefore target either the human host or the fly vector. indeed, suppression of one step in the parasite developmental cycle could ab ...	2015	26029185
a metagenomic survey of viral abundance and diversity in mosquitoes from hubei province.	mosquitoes as one of the most common but important vectors have the potential to transmit or acquire a lot of viruses through biting, however viral flora in mosquitoes and its impact on mosquito-borne disease transmission has not been well investigated and evaluated. in this study, the metagenomic techniquehas been successfully employed in analyzing the abundance and diversity of viral community in three mosquito samples from hubei, china. among 92,304 reads produced through a run with 454 gs fl ...	2015	26030271
incongruent nuclear and mitochondrial genetic structure of new world screwworm fly populations due to positive selection of mutations associated with dimethyl- and diethyl-organophosphates resistance.	livestock production is an important economic activity in brazil, which has been suffering significant losses due to the impact of parasites. the new world screwworm (nws) fly, cochliomyia hominivorax, is an ectoparasite and one of the most important myiasis-causing flies endemic to the americas. the geographic distribution of nws has been reduced after the implementation of the sterile insect technique (sit), being eradicated in north america and part of central america. in south america, c. ho ...	2015	26030866
a case of diabetic ketoacidosis following chikungunya virus infection.	chikungunya is a mosquito-borne viral disease that has recently become endemic in the caribbean, including the island of puerto rico. we present the case of a 50-year-old puerto rican man who traveled to st. louis for business and was diagnosed with acute chikungunya virus infection with atypical features causing diabetic ketoacidosis. this case highlights the need to keep tropical infectious diseases on the differential diagnosis in appropriate individuals and the ways in which tropical infecti ...	2015	26033023
rna interference-mediated antiviral defense in insects.	small interfering rna (sirna)-mediated rna interference (rnai) pathways are critical for the detection and inhibition of rna virus replication in insects. recent work has also implicated rnai pathways in the establishment of persistent virus infections and in the control of dna virus replication. accumulating evidence suggests that diverse double-stranded rnas produced by rna and dna viruses can trigger rnai responses yet many viruses have evolved mechanisms to inhibit rnai defenses. therefore, ...	0	26034705
analysing chemical attraction of gravid anopheles gambiae sensu stricto with modified bg-sentinel traps.	cues that guide gravid anopheles gambiae sensu lato to oviposition sites can be manipulated to create new strategies for monitoring and controlling malaria vectors. however, progress towards identifying such cues is slow in part due to the lack of appropriate tools for investigating long-range attraction to putative oviposition substrates. this study aimed to develop a relatively easy-to-use bioassay system that can effectively analyse chemical attraction of gravid anopheles gambiae sensu strict ...	2015	26036270
emerging virus diseases: can we ever expect the unexpected?	emerging virus diseases are a major threat to human and veterinary public health. with new examples occurring approximately one each year, the majority are viruses originating from an animal host. of the many factors responsible, changes to local ecosystems that perturb the balance between pathogen and principal host species is one of the major drivers, together with increasing urbanization of mankind and changes in human behavior. many emerging viruses have rna genomes and as such are capable o ...	2012	26038413
two disciplines, one priority: the seamless integration of human and veterinary microbiology is urgent.		0	26038422
a threshold analysis of dengue transmission in terms of weather variables and imported dengue cases in australia.	dengue virus (denv) transmission in australia is driven by weather factors and imported dengue fever (df) cases. however, uncertainty remains regarding the threshold effects of high-order interactions among weather factors and imported df cases and the impact of these factors on autochthonous df. a time-series regression tree model was used to assess the threshold effects of natural temporal variations of weekly weather factors and weekly imported df cases in relation to incidence of weekly auto ...	2013	26038449
the rift valley fever accessory proteins nsm and p78/nsm-gn are distinct determinants of virus propagation in vertebrate and invertebrate hosts.	rift valley fever virus (rvfv) is an enzootic virus circulating in africa that is transmitted to its vertebrate host by a mosquito vector and causes severe clinical manifestations in humans and ruminants. rvfv has a tripartite genome of negative or ambisense polarity. the m segment contains five in-frame aug codons that are alternatively used for the synthesis of two major structural glycoproteins, gn and gc, and at least two accessory proteins, nsm, a 14-kda cytosolic protein, and p78/nsm-gn, a ...	2014	26038497
dengue fever in china: an emerging problem demands attention.		2015	26038760
factors responsible for the emergence of arboviruses; strategies, challenges and limitations for their control.	slave trading of africans to the americas, during the 16th to the 19th century was responsible for the first recorded emergence in the new world of two arthropod-borne viruses (arboviruses), yellow fever virus and dengue virus. many other arboviruses have since emerged from their sylvatic reservoirs and dispersed globally due to evolving factors that include anthropological behaviour, commercial transportation and land-remediation. here, we outline some characteristics of these highly divergent ...	2015	26038768
prolyl oligopeptidase from the blood fluke schistosoma mansoni: from functional analysis to anti-schistosomal inhibitors.	blood flukes of the genus schistosoma cause schistosomiasis, a parasitic disease that infects over 240 million people worldwide, and for which there is a need to identify new targets for chemotherapeutic interventions. our research is focused on schistosoma mansoni prolyl oligopeptidase (smpop) from the serine peptidase family s9, which has not been investigated in detail in trematodes.	2015	26039195
human-mediated marine dispersal influences the population structure of aedes aegypti in the philippine archipelago.	dengue virus (denv) is an extraordinary health burden on global scale, but still lacks effective vaccine. the philippines is endemic for dengue fever, but massive employment of insecticides favored the development of resistance mutations in its major vector, aedes aegypti. alternative vector control strategies consist in releasing artificially modified mosquitos in the wild, but knowledge on their dispersal ability is necessary for a successful implementation. despite being documented that ae. a ...	2015	26039311
sequence-independent characterization of viruses based on the pattern of viral small rnas produced by the host.	virus surveillance in vector insects is potentially of great benefit to public health. large-scale sequencing of small and long rnas has previously been used to detect viruses, but without any formal comparison of different strategies. furthermore, the identification of viral sequences largely depends on similarity searches against reference databases. here, we developed a sequence-independent strategy based on virus-derived small rnas produced by the host response, such as the rna interference ...	2015	26040701
evaluation of the efficacy, potential for vector transmission, and duration of immunity of mp-12, an attenuated rift valley fever virus vaccine candidate, in sheep.	rift valley fever virus (rvfv) causes serious disease in ruminants and humans in africa. in north america, there are susceptible ruminant hosts and competent mosquito vectors, yet there are no fully licensed animal vaccines for this arthropod-borne virus, should it be introduced. studies in sheep and cattle have found the attenuated strain of rvfv, mp-12, to be both safe and efficacious based on early testing, and a 2-year conditional license for use in u.s. livestock has been issued. the purpos ...	2015	26041042
prototype early warning systems for vector-borne diseases in europe.	globalization and environmental change, social and demographic determinants and health system capacity are significant drivers of infectious diseases which can also act as epidemic precursors. thus, monitoring changes in these drivers can help anticipate, or even forecast, an upsurge of infectious diseases. the european environment and epidemiology (e3) network has been built for this purpose and applied to three early warning case studies: (1) the environmental suitability of malaria transmissi ...	2015	26042370
the genome of diuraphis noxia, a global aphid pest of small grains.	the russian wheat aphid, diuraphis noxia kurdjumov, is one of the most important pests of small grains throughout the temperate regions of the world. this phytotoxic aphid causes severe systemic damage symptoms in wheat, barley, and other small grains as a direct result of the salivary proteins it injects into the plant while feeding.	2015	26044338
effects of α,β-unsaturated lactones on larval survival and gut trypsin as well as oviposition response of aedes aegypti.	lactones are organic cyclic esters that have been described as larvicides against aedes aegypti and as components of oviposition pheromone of culex quinquefasciatus. this work describes the effect of six α,β-unsaturated lactones (5a-5f) on survival of a. aegypti fourth instar larvae (l4). it is also reported the effects of the lactones on l4 gut trypsin activity and oviposition behavior of a. aegypti females. five lactones were able to kill l4 being the lactones 5a (lc50 of 39.05 ppm), 5e (lc50 ...	2015	26044355
germ line transformation and in vivo labeling of nuclei in diptera: report on megaselia abdita (phoridae) and chironomus riparius (chironomidae).	to understand how and when developmental traits of the fruit fly drosophila melanogaster originated during the course of insect evolution, similar traits are functionally studied in variably related satellite species. the experimental toolkit available for relevant fly models typically comprises gene expression and loss as well as gain-of-function analyses. here, we extend the set of available molecular tools to piggybac-based germ line transformation in two satellite fly models, megaselia abdit ...	2015	26044750
molecular analysis of atypical family 18 chitinase from fujian oyster crassostrea angulata and its physiological role in the digestive system.	chitinolytic enzymes have an important physiological significance in immune and digestive systems in plants and animals, but chitinase has not been identified as having a role in the digestive system in molluscan. in our study, a novel chitinase homologue, named ca-chit, has been cloned and characterized as the oyster crassostrea angulate. the 3998bp full-length cdna of ca-chit consisted of 23bp 5-utr, 3288 orf and 688bp 3-utr. the deduced amino acids sequence shares homologue with the chitinase ...	2015	26046992
carbamate and pyrethroid resistance in the akron strain of anopheles gambiae.	insecticide resistance in the malaria vector, anopheles gambiae, is a serious problem, epitomized by the multi-resistant akron strain, originally isolated in the country of benin. here we report resistance in this strain to pyrethroids and ddt (13-fold to 35-fold compared to the susceptible g3 strain), but surprisingly little resistance to etofenprox, a compound sometimes described as a "pseudo-pyrethroid." there was also strong resistance to topically-applied commercial carbamates (45-fold to 8 ...	2015	26047119
volatile semiochemical-conditioned attraction of the male yellow fever mosquito, aedes aegypti, to human hosts.	we investigated the olfactory responses of male mosquitoes to kairomones of vertebrate hosts in a dual-port olfactometer. the behavioral responses of unmated and mated male and female mosquitoes from one to ten days old to human odors were compared to the odors of different human hosts. to evaluate the relationship between the age of male mosquitoes and their responses, we performed experiments with males at different ages. unmated ae. aegypti males, one to two days old, did not fly upwind to hu ...	2015	26047178
new baseline environmental assessment of mosquito ecology in northern haiti during increased urbanization.	the catastrophic 2010 earthquake in port-au-prince, haiti, led to the large-scale displacement of over 2.3 million people, resulting in rapid and unplanned urbanization in northern haiti. this study evaluated the impact of this unplanned urbanization on mosquito ecology and vector-borne diseases by assessing land use and change patterns. land-use classification and change detection were carried out on remotely sensed images of the area for 2010 and 2013. change detection identified areas that we ...	0	26047183
dengue virus detection in aedes aegypti larvae from southeastern brazil.	the transmission of dengue, the most important arthropod-borne viral disease in brazil, has been intensified over the past decades, along with the accompanying expansion and adaptation of its aedes vectors. in the present study, we mapped dengue vectors in ouro preto and ouro branco, minas gerais, by installing ovitraps in 32 public schools. the traps were examined monthly between september, 2011 through july, 2012 and november, 2012 to april, 2013. the larvae were reared until the fourth stadiu ...	2015	26047186
development and validation of an arthropod maceration protocol for zoonotic pathogen detection in mosquitoes and fleas.	arthropod-borne diseases remain a pressing international public health concern. while progress has been made in the rapid detection of arthropod-borne pathogens via quantitative real-time (qpcr), or even hand-held detection devices, a simple and robust maceration and nucleic acid extraction method is necessary to implement biosurveillance capabilities. in this study, a comparison of maceration techniques using five types of beads followed by nucleic acid extraction and detection were tested usin ...	2015	26047188
lethal ovitrap deployment for aedes aegypti control: potential implications for non-target organisms.	in australia, dengue control combines source reduction with lethal ovitraps to reduce aedes aegypti populations during outbreaks. lethal ovitraps are considered a sustainable and environmentally friendly method of controlling container-inhabiting mosquitoes, however, to-date, this claim has not been quantified. this study assesses the potential impact of lethal ovitraps on non-target organisms when used to control ae. aegypti in tropical australia. for retention of specimens, we substituted stan ...	2015	26047194
the effect of photoperiod on life history and blood-feeding activity in aedes albopictus and aedes aegypti (diptera: culicidae).	several studies have examined how climatic variables such as temperature and precipitation may affect life history traits in mosquitoes that are important to disease transmission. despite its importance as a seasonal cue in nature, studies investigating the influence of photoperiod on such traits are relatively few. this study aims to investigate how photoperiod alters life history traits, survival, and blood-feeding activity in aedes albopictus (skuse) and aedes aegypti (linnaeus). we performed ...	2015	26047197
characterization of adelphocoris suturalis (hemiptera: miridae) transcriptome from different developmental stages.	adelphocoris suturalis is one of the most serious pest insects of bt cotton in china, however its molecular genetics, biochemistry and physiology are poorly understood. we used high throughput sequencing platform to perform de novo transcriptome assembly and gene expression analyses across different developmental stages (eggs, 2(nd) and 5(th) instar nymphs, female and male adults). we obtained 20 gb of clean data and revealed 88,614 unigenes, including 23,830 clusters and 64,784 singletons. thes ...	2015	26047353
targeted release and fractionation reveal glucuronylated and sulphated n- and o-glycans in larvae of dipteran insects.	mosquitoes are important vectors of parasitic and viral diseases with anopheles gambiae transmitting malaria and aedes aegypti spreading yellow and dengue fevers. using two different approaches (solid-phase extraction and reversed-phase or hydrophilic interaction hplc fractionation followed by maldi-tof ms or permethylation followed by nsi-ms), we examined the n-glycans of both a. gambiae and a. aegypti larvae and demonstrate the presence of a range of paucimannosidic glycans as well as bi- and ...	2015	26047717
a standard cytogenetic map of culex quinquefasciatus polytene chromosomes in application for fine-scale physical mapping.	southern house mosquito culex quinquefasciatus belongs to the c. pipiens cryptic species complex, with global distribution and unclear taxonomy. mosquitoes of the complex can transmit human and animal pathogens, such as filarial worm, west nile virus and avian malarial plasmodium. physical gene mapping is crucial to understanding genome organization, function, and systematic relationships of cryptic species, and is a basis for developing new vector control strategies. however, physical mapping w ...	2015	26048143
assessing the epidemiological effect of wolbachia for dengue control.	dengue viruses cause more human morbidity and mortality than any other arthropod-borne virus. dengue prevention relies mainly on vector control; however, the failure of traditional methods has promoted the development of novel entomological approaches. although use of the intracellular bacterium wolbachia to control mosquito populations was proposed 50 years ago, only in the past decade has its use as a potential agent of dengue control gained substantial interest. here, we review evidence that ...	2015	26051887
the venomous cocktail of the vampire snail colubraria reticulata (mollusca, gastropoda).	hematophagy arose independently multiple times during metazoan evolution, with several lineages of vampire animals particularly diversified in invertebrates. however, the biochemistry of hematophagy has been studied in a few species of direct medical interest and is still underdeveloped in most invertebrates, as in general is the study of venom toxins. in cone snails, leeches, arthropods and snakes, the strong target specificity of venom toxins uniquely aligns them to industrial and academic pur ...	2015	26054852
proteomic analysis of immunogenic proteins from salivary glands of aedes aegypti.	humans develop anti-salivary proteins after arthropod bites or exposure to insect salivary proteins. this reaction indicates that vector bites have a positive effect on the host immune response, which can be used as epidemiological markers of exposure to the vector. our previous study identified two immunogenic proteins with molecular weights of 31 kda and 56 kda from salivary gland extract (sge) of aedes aegypti that cross-reacted with serum samples from dengue hemorrhagic fever (dhf) patients ...	2015	26054892
functional development of carbon dioxide detection in the maxillary palp of anopheles gambiae.	olfactory information drives several behaviours critical for the survival and persistence of insect pests and vectors. insect behaviour is variable, linked to their biological needs, and regulated by physiological dynamics. for mosquitoes, co2 is an important cue that signifies the presence of a host, and which elicits activation and attraction. to investigate the genetic basis of olfactory modulation in mosquitoes, we assayed changes in co2 detection from receptor gene expression through physio ...	2015	26056246
a critical role for clsp2 in the modulation of antifungal immune response in mosquitoes.	entomopathogenic fungi represent a promising class of bio-insecticides for mosquito control. thus, detailed knowledge of the molecular mechanisms governing anti-fungal immune response in mosquitoes is essential. in this study, we show that clsp2 is a modulator of immune responses during anti-fungal infection in the mosquito aedes aegypti. with a fungal infection, the expression of the clsp2 gene is elevated. clsp2 is cleaved upon challenge with beauveria bassiana conidia, and the liberated clsp2 ...	2015	26057557
characterization of the doublesex gene within the culex pipiens complex suggests regulatory plasticity at the base of the mosquito sex determination cascade.	the doublesex gene controls somatic sexual differentiation of many metazoan species, including the malaria mosquito anopheles gambiae and the dengue and yellow fever vector aedes aegypti (diptera: culicidae). as in other studied dipteran dsx homologs, the gene maintains functionality via evolutionarily conserved protein domains and sex-specific alternative splicing. the upstream factors that regulate splicing of dsx and the manner in which they do so however remain variable even among closely re ...	2015	26058583
abundant aedes (stegomyia) aegypti aegypti mosquitoes in the 2014 dengue outbreak area of mozambique.	in early 2014, dengue cases were reported from northern mozambique, 30 years after the last outbreak. we identified potential dengue vector species in three northern towns, pemba, nampula and nacala, and one southern town, maputo, during the outbreak in april 2014. a major dengue vector species, aedes (stegomyia) aegypti, was found in all these towns. the dominant vector subspecies in the northern towns was aedes aegypti aegypti, while ae. aegypti formosus was dominant in maputo. considering the ...	2015	26060423
recent advances in dengue: relevance to puerto rico.	dengue represents an increasingly important public health challenge in puerto rico, with recent epidemics in 2007, 2010, and 2012-2013. although recent advances in dengue vaccine development offer hope for primary prevention, the role of health professionals in the diagnosis and management of dengue patients is paramount. case definitions for dengue, dengue with warning signs, and severe dengue provide a framework to guide clinical decision-making. furthermore, the differentiation between dengue ...	0	26061055
the spatiotemporal trajectory of a dengue epidemic in a medium-sized city.	understanding the transmission dynamics of infectious diseases is important to allow for improvements of control measures. to investigate the spatiotemporal pattern of an epidemic dengue occurred at a medium-sized city in the northeast region of brazil in 2009, we conducted an ecological study of the notified dengue cases georeferenced according to epidemiological week (ew) and home address. kernel density estimation and space-time interaction were analysed using the knox method. the evolution o ...	2015	26061236
the chikungunya virus capsid protein contains linear b cell epitopes in the n- and c-terminal regions that are dependent on an intact c-terminus for antibody recognition.	chikungunya virus (chikv) is an arthropod-borne agent that causes severe arthritic disease in humans and is considered a serious health threat in areas where competent mosquito vectors are prevalent. chikv has recently been responsible for several millions of cases of disease, involving over 40 countries. the recent re-emergence of chikv and its potential threat to human health has stimulated interest in better understanding of the biology and pathogenesis of the virus, and requirement for impro ...	2015	26061335
a defensin from the model beetle tribolium castaneum acts synergistically with telavancin and daptomycin against multidrug resistant staphylococcus aureus.	the red flour beetle tribolium castaneum is a common insect pest and has been established as a model beetle to study insect development and immunity. this study demonstrates that defensin 1 from t. castaneum displays in vitro and in vivo antimicrobial activity against drug resistant staphylococcus aureus strains. the minimum inhibitory concentration (mic) of defensin 1 against 11 reference and clinical staphylococcal isolates was between 16-64 μg/ml. the putative mode of action of the defensin p ...	2015	26062137
evaluation of the toxicity and repellence of an organic fatty acids mixture (c8910) against insecticide susceptible and resistant strains of the major malaria vector anopheles funestus giles (diptera: culicidae).	malaria vector control relies principally on the use of insecticides, especially pyrethroids. because of the increasing occurrence of insecticide resistance in target vector populations, the development of new insecticides, particularly those with novel modes of action, is particularly important, especially in terms of managing insecticide resistance. the c8910 formulation is a patented mixture of compounds comprising straight-chain octanoic, nonanoic and decanoic saturated fatty acids. this com ...	2015	26062763
electrophysiological and behavioral characterization of bioactive compounds of the thymus vulgaris, cymbopogon winterianus, cuminum cyminum and cinnamomum zeylanicum essential oils against anopheles gambiae and prospects for their use as bednet treatments.	laboratory and field studies showed that repellent, irritant and toxic actions of common public health insecticides reduce human-vector contact and thereby interrupt disease transmission. one of the more effective strategies to reduce disease risk involves the use of long-lasting treated bednets. however, development of insecticide resistance in mosquito populations makes it imperative to find alternatives to these insecticides. our previous study identified four essential oils as alternatives t ...	2015	26063119
green-synthesized silver nanoparticles as a novel control tool against dengue virus (den-2) and its primary vector aedes aegypti.	dengue is an arthropod-borne viral infection mainly vectored through the bite of aedes mosquitoes. recently, its transmission has strongly increased in urban and semi-urban areas of tropical and sub-tropical regions worldwide, becoming a major international public health concern. there is no specific treatment for dengue. its prevention and control solely depends on effective vector control measures. in this study, we proposed the green-synthesis of silver nanoparticles (agnp) as a novel and eff ...	2015	26063530
an unusual role for doublesex in sex determination in the dipteran sciara.	the gene doublesex, which is placed at the bottom of the sex-determination gene cascade, plays the ultimate discriminatory role for sex determination in insects. in all insects where this gene has been characterized, the dsx premessenger rna (pre-mrna) follows a sex-specific splicing pattern, producing male- and female-specific mrnas encoding the male-dsxm and female-dsxf proteins, which determine male and female development, respectively. this article reports the isolation and characterization ...	2015	26063659
entomological indicators during transmission season of dengue in silvassa (india).	the entomological surveillance was conducted in urban, semi-urban/slum, industrial and residential areas during main transmission period from june to november 2012. in residential sites house index was 41.7-35.0, breteau index 71.7-136.7 and container index 11.6-20.2. during transmission period all the values ware much higher than the threshold level. the causes of high values of entomological indicator appeared to be rapid industrialization, unawareness of the conditions or factors that can exa ...	2013	26064033
intricate predatory decisions by a mosquito-specialist spider from malaysia.	paracyrba wanlessi is a southeast asian jumping spider (salticidae) that lives in the hollow internodes of fallen bamboo and preys on the larvae, pupae and adults of mosquitoes. in contrast to evarcha culicivora, an east african salticid that is also known for actively targeting mosquitoes as preferred prey, there was no evidence of p. wanlessi choosing mosquitoes on the basis of species, sex or diet. however, our findings show that p. wanlessi chooses mosquitoes significantly more often than a ...	2014	26064534
conidia of the insect pathogenic fungus, metarhizium anisopliae, fail to adhere to mosquito larval cuticle.	adhesion of conidia of the insect pathogenic fungus, metarhizium anisopliae, to the arthropod host cuticle initially involves hydrophobic forces followed by consolidation facilitated by the action of extracellular enzymes and secretion of mucilage. gene expression analysis and atomic force microscopy were used to directly quantify recognition and adhesion between single conidia of m. anisopliae and the cuticle of the aquatic larval stage of aedes aegypti and a representative terrestrial host, te ...	2014	26064542
distinct sets of piwi proteins produce arbovirus and transposon-derived pirnas in aedes aegypti mosquito cells.	the piwi-interacting rna (pirna) pathway is essential for transposon silencing in many model organisms. its remarkable efficiency relies on a sophisticated amplification mechanism known as the ping-pong loop. in alphavirus-infected aedes mosquitoes, pirnas with sequence features that suggest ping-pong-dependent biogenesis are produced from viral rna. the piwi family in aedes mosquitoes is expanded when compared to other model organisms, raising the possibility that individual piwi proteins have ...	2015	26068474
eilat virus induces both homologous and heterologous interference.	most alphaviruses are mosquito-borne and exhibit a broad host range, infecting many different vertebrates including birds, rodents, equids, and humans. occasionally, alphaviruses can spill over into the human population and cause disease characterized by debilitating arthralgia or fatal encephalitis. recently, a unique alphavirus, eilat virus (eilv), was described that readily infects mosquito but not vertebrate cell lines. here, we investigated the ability of eilv to induce superinfection exclu ...	2015	26068885
relationship between insecticide resistance and kdr mutations in the dengue vector aedes aegypti in southern china.	aedes aegypti is an important vector for dengue virus and thus has been targeted with pyrethroid insecticides in many areas of the world. as such, resistance has been detected to several of these insecticides, including in china, but the mechanisms of the resistance are not well understood in this country.	2015	26068925
development of a hamster model for chikungunya virus infection and pathogenesis.	chikungunya virus is transmitted by mosquitoes and causes severe, debilitating infectious arthritis in humans. the need for an animal model to study the disease process and evaluate potential treatments is imminent as the virus continues its spread into novel geographic locations. golden hamsters (mesocricetus auratus) are often used as outbred laboratory animal models for arboviral diseases. here we demonstrate that hamsters inoculated with chikungunya virus developed viremia and histopathologi ...	2015	26070211
evidence of two mechanisms involved in bacillus thuringiensis israelensis decreased toxicity against mosquito larvae: genome dynamic and toxins stability.	biopesticides based on bacillus thuringiensis israelensis are the most used and most successful around the world. this bacterium is characterized by a dynamic genome able to win or lose genetic materials which leads to a decrease in its effectiveness. the detection of such phenomena is of great importance to monitor the stability of b. thuringiensis strains in industrial production processes of biopesticides. new local b. thuringiensis israelensis isolates were investigated. they present variabl ...	2015	26070692
rice ragged stunt virus-induced apoptosis affects virus transmission from its insect vector, the brown planthopper to the rice plant.	most plant viruses that seriously damage agricultural crops are transmitted by insects. however, the mechanisms enabling virus transmission by insect vectors are poorly understood. the brown planthopper (nilaparvata lugens) is one of the most serious rice pests, causing extensive damage to rice plants by sucking the phloem sap and transmitting viruses, including rice ragged stunt virus (rrsv). in this study, we investigated the mechanisms of rrsv transmission from its insect vector to the rice p ...	2015	26073458
function and evolution of micrornas in eusocial hymenoptera.	the emergence of eusociality ("true sociality") in several insect lineages represents one of the most successful evolutionary adaptations in the animal kingdom in terms of species richness and global biomass. in contrast to solitary insects, eusocial insects evolved a set of unique behavioral and physiological traits such as reproductive division of labor and cooperative brood care, which likely played a major role in their ecological success. the molecular mechanisms that support the social reg ...	2015	26074950
asymmetric effects of native and exotic invasive shrubs on ecology of the west nile virus vector culex pipiens (diptera: culicidae).	exotic invasive plants alter the structure and function of native ecosystems and may influence the distribution and abundance of arthropod disease vectors by modifying habitat quality. this study investigated how invasive plants alter the ecology of culex pipiens, an important vector of west nile virus (wnv) in northeastern and midwestern regions of the united states.	2015	26076589
complete dosage compensation in anopheles stephensi and the evolution of sex-biased genes in mosquitoes.	complete dosage compensation refers to hyperexpression of the entire x or z chromosome in organisms with heterogametic sex chromosomes (xy male or zw female) in order to compensate for having only one copy of the x or z chromosome. recent analyses suggest that complete dosage compensation, as in drosophila melanogaster, may not be the norm. there has been no systematic study focusing on dosage compensation in mosquitoes. however, analysis of dosage compensation in anopheles mosquitoes provides o ...	2015	26078263
characterization of anopheles gambiae (african malaria mosquito) ferritin and the effect of iron on intracellular localization in mosquito cells.	ferritin is a 24-subunit molecule, made up of heavy chain (hc) and light chain (lc) subunits, which stores and controls the release of dietary iron in mammals, plants, and insects. in mosquitoes, dietary iron taken in a bloodmeal is stored inside ferritin. our previous work has demonstrated the transport of dietary iron to the ovaries via ferritin during oogenesis. we evaluated the localization of ferritin subunits inside ccl-125 [aedes aegypti linnaeus (diptera: culicidae), yellow fever mosquit ...	2015	26078302
identification and knockdown of the olfactory receptor (orco) in gypsy moth, lymantria dispar.	the gypsy moth, lymantria dispar, is an important economic pest that causes large-scale damage to forests worldwide. because of its important role in initiating and controlling insect behavior, olfaction-and olfaction-based pest management-has drawn increasing attention from entomologists. in this study, we identified the gene that encodes the olfactory receptor co-receptor (orco). through amino acid sequence alignment, we found that ldisorco shares high identity with other orco proteins from di ...	2015	26078719
assessment of arbovirus surveillance 13 years after introduction of west nile virus, united states.	before 1999, the united states had no appropriated funding for arboviral surveillance, and many states conducted no such surveillance. after emergence of west nile virus (wnv), federal funding was distributed to state and selected local health departments to build wnv surveillance systems. the council of state and territorial epidemiologists conducted assessments of surveillance capacity of resulting systems in 2004 and in 2012; the assessment in 2012 was conducted after a 61% decrease in federa ...	0	26079471
localization and role of inward rectifier k(+) channels in malpighian tubules of the yellow fever mosquito aedes aegypti.	malpighian tubules of adult female yellow fever mosquitoes aedes aegypti express three inward rectifier k(+) (kir) channel subunits: aekir1, aekir2b and aekir3. here we 1) elucidate the cellular and membrane localization of these three channels in the malpighian tubules, and 2) characterize the effects of small molecule inhibitors of aekir1 and aekir2b channels (vu compounds) on the transepithelial secretion of fluid and electrolytes and the electrophysiology of isolated malpighian tubules. usin ...	2015	26079629
circumsporozoite protein as a potential target for antimalarials.	since the discovery of circumsporozoite protein (csp), a major sporozoite surface antigen, by ruth nussenzweig and victor nussenzweig in the early 1980s, the role of csp in protection against malaria has been extensively investigated. several monoclonal antibodies against csp have been generated to date, with some of them mediating antimalarial protection upon passive transfer into animals. genetically engineered transgenic mosquitoes producing the anti-csp antibody have recently been generated ...	2015	26081442