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coexistence of philodina roseola (rotifera: bdelloidea) with larvae of aedes aegypti in india.the vector mosquitoes, aedes aegypti and aedes albopictus of dengue and chikungunya fever are closely associated with human habitations and adapted to feed on human blood. they undergo larval and pupal development in natural and artificial freshwater collections in the urban and peri-urban environment. although reports are available about the feeding behaviour of the thriving mosquito larvae, much information is still required to understand the successful survival of aedes mosquitoes in small an ...201425134889
oral susceptibility of aedes aegypti (diptera: culicidae) from senegal for dengue serotypes 1 and 3 viruses.to investigate the potential for domestic and wild populations of aedes aegypti from dakar and kedougou to develop a disseminated infection after exposure to denv-3 and denv-1.201425134541
aedes aegypti larvicide from the ethanolic extract of piper nigrum black peppercorns.due to unavailability of a vaccine and a specific cure to dengue, the focus nowadays is to develop an effective vector control method against the female aedes aegypti mosquito. this study aims to determine the larvicidal fractions from piper nigrum ethanolic extracts (pnpcme) and to elucidate the identity of the bioactive compounds that comprise these larvicidal fractions. larvicidal assay was performed by subjecting 3rd to 4th a. aegypti instar larvae to pnpcme of p. nigrum. the pnpcme exhibite ...201525118563
exploring new thermal fog and ultra-low volume technologies to improve indoor control of the dengue vector, aedes aegypti (diptera: culicidae).control of the mosquito vector, aedes aegypti (l.), inside human habitations must be performed quickly and efficiently to reduce the risk of transmission during dengue outbreaks. as part of abroad study to assess the efficacy of dengue vector control tools for the u.s. military, two pesticide delivery systems (ultra-low volume [ulv] and thermal fog) were evaluated for their ability to provide immediate control of ae. aegypti mosquitoes with a contact insecticide inside simulated urban structures ...201425118418
chemical composition, larvicidal, and biting deterrent activity of essential oils of two subspecies of tanacetum argenteum (asterales: asteraceae) and individual constituents against aedes aegypti (diptera: culicidae).water-distilled essential oils from dried aerial parts of tanacetum argenteum (lam.) willd. subsp. argenteum (lam.) and t. argenteum (lam.) willd. subsp. canum (c. koch) grierson were analyzed by gas chromatography and gas chromatography-mass spectrometry. in total, 27 and 32 components were identified representing 97.2 and 98.7% of essential oils of subsp. argenteum and canum, respectively. main compounds of t. argenteum subsp. argenteum were alpha-pinene (67.9%) and beta-pinene (4.8%), whereas ...201425118415
control of larval and egg development in aedes aegypti with rna interference against juvenile hormone acid methyl transferase.rna interference (rnai) is a powerful approach for elucidating gene functions in a variety of organisms, including mosquitoes and many other insects. little has been done, however, to harness this approach in order to control adult and larval mosquitoes. juvenile hormone (jh) plays a pivotal role in the control of reproduction in adults and metamorphism in larval mosquitoes. this report describes an approach to control aedes aegypti using rnai against jh acid methyl transferase (aeajhamt), the u ...201425111689
biological control of mosquitoes in scrap tires in brownsville, texas, usa and matamoros, tamaulipas, mexico.dengue periodically circulates in southern texas and neighboring tamaulipas, mexico; thus, a closer examination of human and vector ecology at the northern limits of north american transmission may improve prevention activities. scrap tires produce large mosquito populations and increase the risk of dengue transmission. some households choose not to pay tire disposal fees, and many tires are illegally dumped in residential areas. biological control may provide low-cost and environmentally friend ...201425102598
mosquito communities in nova iguaçu natural park, rio de janeiro, brazil.abstract. in order to gather information on the culicid fauna of nova iguaçu municipal park, rio de janeiro, brazil, adult and immature stages were collected with the centers for disease control and prevention miniature light traps, and dippers and suction tubes, respectively. in all, 828 adult and 990 immature specimens were collected belonging to 12 genera. among the species collected were aedes aegypti, ae. albopictus, ae. fluviatilis, ae. scapularis, haemagogus leucocelaenus, and psorophora ...201425102590
standardization of a bottle assay--an indigenous method for laboratory and field monitoring of insecticide resistance and comparison with who adult susceptibility test.the who adult susceptibility test is in use for insecticide resistance monitoring. presently, materials are being imported from the universiti sains malaysia, malaysia and sometimes it is cost prohibitive. as an alternative, we present here a method of bottle bioassay using indigenous material. different aspects related to the assay were studied and validated in the field. bottle assay was standardized in the laboratory by using locally sourced material and laboratory-maintained insecticide-susc ...201425098343
repellency of essential oils extracted from thai native plants against aedes aegypti (linn.) and culex quinquefasciatus (say).repellent activity of essential oils derived from 10 thai native plants, belonging to three families were evaluated against female aedes aegypti and culex quinquefasciatus and to compare them with a commercial chemical repellents (deet; n,n-diethyl-3-methylbenzamide 20% w/w; sketolene shield). each test repellent was applied at 1, 5, and 10% concentrations for testing by arm in cage method. the results showed significant differences in repellency among the repellents by mosquito species. the pro ...201425088471
[dengue fever in mainland france].dengue fever is the most widespread distributed vector borne viral disease. it is transmitted through the bites of aedes aegypti and aedes albopictus mosquitoes. with the expansion of aedes albopictus and increasing travel exchange, it is no longer limited to the tropical zone and transmission has been documented in temperate areas. in mainland france, where aedes albopictus has been present and disseminating since 2004, 2 episodes of autochthonous transmission occurred in 2010 and in 2013. cont ...201425080833
optimization of reaction conditions to fabricate nano-silver using couroupita guianensis aubl. (leaf & fruit) and its enhanced larvicidal effect.currently bioactive principles of plants and their nanoproducts have been extensively studied in agriculture and medicine. in this study couroupita guianensis aubl. leaf and fruit extracts were selected for rapid and cost-effective synthesis of silver nanoparticles (leaf-lagnps and fruit-fagnps). various physiological conditions such as temperature, ph, concentration of metal ions, stoichiometric proportion of reaction mixture and reaction time showed influence on the size, dispersity and synthe ...201525062056
evaluation of seed extracts from plants found in the caatinga biome for the control of aedes aegypti.dengue fever, currently the most important arbovirus, is transmitted by the bite of the aedes aegypti mosquito. given the absence of a prophylactic vaccine, the disease can only be controlled by combating the vector insect. however, increasing reports of resistance and environmental damage caused by insecticides have led to the urgent search for new safer alternatives. in this regard, plants stand out as a source of easy-to-obtain biodegradable insecticide molecules. twenty (20) plant seed extra ...201425056942
the excretion of nacl and kcl loads in mosquitoes. 1. control data.the handling of na(+) and k(+) loads was investigated in isolated malpighian tubules and in whole mosquitoes of aedes aegypti. isolated malpighian tubules bathed in na(+)-rich ringer solution secreted na(+)-rich fluid, and tubules bathed in k(+)-rich ringer solution secreted k(+)-rich fluid. upon na(+) loading the hemolymph, the mosquito removed 77% the injected na(+) within the next 30 min. the rapid onset and magnitude of this diuresis and the excretion of more na(+) than can be accounted for ...201425056103
toxicity of thiophenes from echinops transiliensis (asteraceae) against aedes aegypti (diptera: culicidae) larvae.structureactivity relationships of nine thiophenes, 2,2': 5',2″-terthiophene (1), 2-chloro-4-[5-(penta-1,3-diyn-1-yl)thiophen-2-yl]but-3-yn-1-yl acetate (2), 4-(2,2'-bithiophen-5-yl)but-3-yne-1,2-diyl diacetate (3), 4-[5-(penta-1,3-diyn-1-yl)thiophen-2-yl]but-3-yne-1,2-diyl diacetate (4), 4-(2,2'-bithiophen-5-yl)-2-hydroxybut-3-yn-1-yl acetate (5), 2-hydroxy-4-[5-(penta-1,3-diyn-1-yl)thiophen-2-yl]but-3-yn-1-yl acetate (6), 1-hydroxy-4-[5-(penta-1,3-diyn-1-yl)thiophen-2-yl]but-3-yn-2-yl acetate ...201425044586
effect of niloticin, a protolimonoid isolated from limonia acidissima l. (rutaceae) on the immature stages of dengue vector aedes aegypti l. (diptera: culicidae).the aim of the present study was to evaluate the mosquitocidal activity of fractions and a compound niloticin from the hexane extract of limonia acidissima l. leaves on eggs, larvae and pupae of aedes aegypti l. (diptera: culicidae). in these bioassays, the eggs, larvae and pupae were exposed to concentrations of 2.5, 5.0, 7.5 and 10.0ppm for fractions and 0.5, 1.0, 1.5 and 2.0ppm for compound. after 24h, the mortality was assessed and the lc50 and lc90 values were calculated for larvae and pupa ...201425019220
assessing dengue infection risk in the southern region of taiwan: implications for control.dengue, one of the most important mosquito-borne diseases, is a major international public health concern. this study aimed to assess potential dengue infection risk from aedes aegypti in kaohsiung and the implications for vector control. here we investigated the impact of dengue transmission on human infection risk using a well-established dengue-mosquito-human transmission dynamics model. a basic reproduction number (r 0)-based probabilistic risk model was also developed to estimate dengue inf ...201525007831
peroxiredoxin 5 from common cutworm (spodoptera litura) acts as a potent antioxidant enzyme.in this study, we describe the cloning and characterization of a prx from the common cutworm spodoptera litura (slprx5). the slprx5 cdna contains an open reading frame of 477 bp encoding a predicted protein of 159 amino acid residues, 16.902 kda, and an isoelectric point of 7.68. furthermore, the deduced amino acid sequence of the slprx5 cdna showed 86% identity to papilio xuthus prx5, 72% to aedes aegypti prx5, and 64-67% to other insect prxs. a phylogenetic analysis further revealed that the d ...201424998343
aedes aegypti (diptera: culicidae): evaluation of natural long-lasting materials containing pyriproxyfen to improve control strategies.natural materials such as beeswax or a paraffin/stearin mixture containing pyriproxyfen and used as a slow release formulation may convert any breeding place into a larvicidal ovitrap for aedes aegypti (l.) control. effectiveness and residual activity of beeswax and paraffin/stearin 1:1 discs containing from 10(-5) to 10(-1) % pyriproxyfen and sticked at the bottom of plastic jars were evaluated for adult emergence inhibition (ei) on late 3rd or early 4th instar a. aegypti larvae. at the initial ...201424974963
guppies as predators of common mosquito larvae in malaysia.observation on predation activities of guppies (poecilia reticulata) on the larvae of three species of mosquito, namely aedes albopictus, aedes aegypti, and culex quinquefasciatus was carried out under laboratory conditions. male and female guppies were used as predators for predation experiments on the 4th instars of mosquito larvae. the daily feeding rates comparing male and female guppies on mosquito larvae were different; the female guppies consumed more mosquito larvae than male guppies did ...201424968669
using garp to predict the range of aedes aegypti in china.dengue fever and dengue hemorrhagic fever are common mosquito-borne diseases in tropical and subtropical regions, and are mainly transmitted by the mosquito aedes aegypti (diptera: culicidae). the international trade of used tires, coupled with its anthropophilic habit, has enabled ae. aegypti to colonise new areas in china. we used genetic algorithum rule-set production (garp) to predict the putative current distribution of ae. aegypti based on data on its distribution 20 years ago and compared ...201424968668
application of quantitative pcr for quantization of densovirus genome.densovirus is classified as invertebrate virus belonging to the subfamily densovirinae of parvoviridae family. this group of viruses infects only insects and several densoviruses have been isolated from indigenous mosquitoes and mosquito cell lines. a number of mosquitoes, especially aedes aegypti and ae. albopictus are important vectors of viruses, which are the major causes of dengue hemorrhagic fever and yellow fever in humans. as densoviruses do not cause any pathology in humans, these virus ...201424964653
factors influencing the feeding response of laboratory-reared aedes aegypti.we evaluated the effects of membrane surface area (cm2), female density, and container/cage size on feeding response in laboratory-reared aedes aegypti. female density did not affect feeding rates at low surface areas, but higher density did significantly increase feeding as surface area increased. females in large, cloth cages fed less compared to those in large, plastic cups. the rate of feeding was higher when using live, anesthetized mice versus a membrane feeding system. the afrims insectar ...201424964652
ecobiosocial community intervention for improved aedes aegypti control using water container covers to prevent dengue: lessons learned from girardot colombia. 201424962850
insecticidal compounds from rhizophoraceae mangrove plants for the management of dengue vector aedes aegypti.mosquito control is facing a threat due to the emergence of resistance to synthetic insecticides. insecticides of botanical origin could serve as potential alternatives in future. larvicidal efficacies of different parts of mangrove plants belonging to rhizophoraceae family were tested against the late iv instar larvae of dengue vector, aedes aegypti.201424947217
frequency of v1016i and f1534c mutations in the voltage-gated sodium channel gene in aedes aegypti in venezuela.the v1016i and f1534c mutations in the voltage-gated sodium channel gene have been associated with resistance to pyrethroids and ddt in aedes aegypti mosquitoes. a study was carried out to determine the frequency of i1016 and c1534 by real-time pcr in five natural populations of ae. aegypti in venezuela during 2008, 2010 and 2012, as well as in a strain selected with 0.14 µg of deltamethrin for 15 generations.201524935645
a spatial model with pulsed releases to compare strategies for the sterile insect technique applied to the mosquito aedes aegypti.we present a simple mathematical model to replicate the key features of the sterile insect technique (sit) for controlling pest species, with particular reference to the mosquito aedes aegypti, the main vector of dengue fever. the model differs from the majority of those studied previously in that it is simultaneously spatially explicit and involves pulsed, rather than continuous, sterile insect releases. the spatially uniform equilibria of the model are identified and analysed. simulations are ...201424929226
polyenylcyclopropane carboxylic esters with high insecticidal activity.pyrethroids are synthetic derivatives of naturally occurring pyrethrum. these molecules are widely used in agriculture for ant, fly and mosquito control and for lawn and garden care. pyrethroids are the optically active esters of 2,2-dimethyl-3-(2-methylpropenyl)-cyclopropane carboxylic acid, also known as chrysanthemic acid. however, their intense use has resulted in the development of resistance in many insect species. herein, specific structural modifications of the pyrethroid scaffold and th ...201524899413
transcriptional analysis of four family 4 p450s in a puerto rico strain of aedes aegypti (diptera: culicidae) compared with an orlando strain and their possible functional roles in permethrin resistance.a field strain of aedes aegypti (l.) was collected from puerto rico in october 2008. based on ld50 values by topical application, the puerto rico strain was 73-fold resistant to permethrin compared with a susceptible orlando strain. in the presence of piperonyl butoxide, the resistance of puerto rico strain of ae. aegypti was reduced to 15-fold, suggesting that cytochrome p450-mediated detoxification is involved in the resistance of the puerto rico strain to permethrin. to determine the cytochro ...201424897853
developing exon-primed intron-crossing (epic) markers for population genetic studies in three aedes disease vectors.aedes aegypti, aedes notoscriptus, and aedes albopictus are important vectors of many arboviruses implicated in human disease such as dengue fever. genetic markers applied across vector species can provide important information on population structure, gene flow, insecticide resistance, and taxonomy, however, robust microsatellite markers have proven difficult to develop in these species and mosquitoes generally. here we consider the utility and transferability of 15 ribosome protein (rp) exon-p ...201524895297
climate change and the potential global distribution of aedes aegypti: spatial modelling using gis and climex.we examined the potential added risk posed by global climate change on the dengue vector aedes aegypti abundance using climex, a powerful tool for exploring the relationship between the fundamental and realised niche of any species. after calibrating the model using data from several knowledge domains, including geographical distribution records, we estimated potential distributions of the mosquito under current and future potential scenarios. the impact of climate change on its potential distri ...201424893017
survival of larvivorous fish used for biological control of aedes aegypti (diptera: culicidae) combined with different larvicides.to evaluate combinations of larvicides and fish which are predators of larvae of aedes aegypti, namely betta splendens, trichogaster trichopterus and poecilia reticulata.201424890120
biofabrication of ag nanoparticles using sterculia foetida l. seed extract and their toxic potential against mosquito vectors and hela cancer cells.a one-step and eco-friendly process for the synthesis of silver-(protein-lipid) nanoparticles (ag-pl nps) (core-shell) has been developed using the seed extract from wild indian almond tree, sterculia foetida (l.) (sterculiaceae). the reaction temperature played a major role in controlling the size and shell formation of nps. the amount of nps synthesized and qualitative characterization was done by uv-vis spectroscopy and transmission electron microscopy (tem), respectively. tem studies exhibit ...201424863217
bacteria as a source of oviposition attractant for aedes aegypti mosquitoes.since a safe and effective mass vaccination program against dengue fever is not presently available, a good way to prevent and control dengue outbreaks depends mainly on controlling the mosquito vectors. aedes aegypti mosquito populations can be monitored and reduced by using ovitraps baited with organic infusions. a series of laboratory experiments were conducted which demonstrated that the bacteria in bamboo leaf infusion produce volatile attractants and contact chemical stimulants attractive ...201424862053
sublethal effects of atrazine and glyphosate on life history traits of aedes aegypti and aedes albopictus (diptera: culicidae).although exposure of mosquito larvae to agricultural chemicals such as herbicides is common and widespread, our understanding of how these chemicals affect mosquito ecology and behavior is limited. this study investigated how an environmentally relevant concentration of two herbicides, atrazine and glyphosate, affects mosquito life history traits. one hundred and fifty (150) first instar aedes (stegomyia) aegypti (l.) or aedes (stegomyia) albopictus (skuse) larvae were reared in 1.6 l of live oa ...201424853538
defense response in non-genomic model species: methyl jasmonate exposure reveals the passion fruit leaves' ability to assemble a cocktail of functionally diversified kunitz-type trypsin inhibitors and recruit two of them against papain.multiplicity of protease inhibitors induced by predators may increase the understanding of a plant's intelligent behavior toward environmental challenges. information about defense mechanisms of non-genomic model plant passion fruit (passiflora edulis sims) in response to predator attack is still limited. here, via biochemical approaches, we showed its flexibility to build-up a broad repertoire of potent kunitz-type trypsin inhibitors (ktis) in response to methyl jasmonate. seven inhibitors (20- ...201424849173
biting deterrence, repellency, and larvicidal activity of ruta chalepensis (sapindales: rutaceae) essential oil and its major individual constituents against mosquitoes.the essential oil from aerial parts of ruta chalepensis l. (sapindales: rutaceae) was obtained by hydrodistillation, and its chemical profile was identified using gas chromatography and gas chromatography-mass spectrometry. compounds, 2-undecanone (43.2%), 2-nonanone (27.9%), and 2-nonyl acetate (10.6%) were the major constituents of the oil. biting deterrent activity of r. chalepensis essential oil at 10 and 50 microg/cm2, 2-undecanone at 8.5 microg/cm2, 2-nonanone at 9 microg/cm2, and 2-nonyl ...201324843931
pesticide-induced release from competition among competing aedes aegypti and aedes albopictus (diptera: culicidae).competitive interactions between mosquitoes aedes aegypti (l.) and aedes albopictus (skuse) may depend on environmental conditions. pesticides may alleviate density-dependent competition for limited food, and a differential species response to sublethal concentrations may modify interspecific competition. we tested the hypothesis that exposure to malathion alters interspecific resource competition between these two species. in the absence of malathion, ae. aegypti survivorship and per capita rat ...201324843928
characterization of the spatial and temporal dynamics of the dengue vector population established in urban areas of fernando de noronha, a brazilian oceanic island.aedes aegypti has played a major role in the dramatic expansion of dengue worldwide. the failure of control programs in reducing the rhythm of global dengue expansion through vector control suggests the need for studies to support more appropriated control strategies. we report here the results of a longitudinal study on ae. aegypti population dynamics through continuous egg sampling aiming to characterize the infestation of urban areas of a brazilian oceanic island, fernando de noronha. the spa ...201424832009
the 2014 fifa world cup: communicable disease risks and advice for visitors to brazil--a review from the latin american society for travel medicine (slamvi).the next fifa world cup will be held in brazil in june-july 2014. around 600,000 international visitors and participants (as well over 3 million domestic travelers) are expected. this event will take place in twelve cities. this event poses specific challenges, given its size and the diversity of attendees, including the potential for the transmission of imported or endemic communicable diseases, especially those that have an increased transmission rate as a result of close human proximity, eg, ...201424821081
oviposition by aedes aegypti and aedes albopictus: influence of congeners and of oviposition site characteristics.we investigated the oviposition behavior of ae. aegypti and ae. albopictus. in particular we examined whether small-scale site characteristics and the presence of conspecifics or congeners altered oviposition by these mosquitoes. various combinations of females of the two species were allowed to oviposit inside cages among either vegetation (potted plants) or structural components (wood and concrete blocks). numbers of eggs deposited per female were compared between species, sides, and treatment ...201424820572
determining the spatial autocorrelation of dengue vector populations: influences of mosquito sampling method, covariables, and vector control.we investigated spatial autocorrelation of female aedes aegypti l. mosquito abundance from bg-sentinel trap and sticky ovitrap collections in cairns, north queensland, australia. bg-sentinel trap collections in 2010 show a significant spatial autocorrelation across the study site and over a smaller spatial extent, while sticky ovitrap collections only indicate a non-significant, weak spatial autocorrelation. the bg-sentinel trap collections were suitable for spatial interpolation using ordinary ...201424820568
excito-repellency of essential oils against an aedes aegypti (l.) field population in thailand.an investigation of the behavioral responses of aedes aegypti (= stegomyia aegypti) to various concentrations of essential oils (2.5, 5, and 10%) extracted from hairy basil (ocimum americanum linn), ginger (zingiber officinale roscoe), lemongrass (cymbopogon citratus stapf), citronella grass (cymbopogon nardus rendle), and plai (zingiber cassumunar roxb) were performed using an excito-repellency test chamber. results showed that ae. aegypti exhibited varying levels of escape response in both the ...201424820563
utilization of larval and pupal detritus by aedes aegypti and aedes albopictus.the utilization of detritus sources by mosquito larvae during development may significantly affect adult life history traits and mosquito population growth. many studies have shown invertebrate carcasses to be an important detritus source in larval habitats, but little is known regarding how invertebrate carcasses are utilized by mosquito larvae. we conducted two studies to investigate the rate of detritus consumption and its effect on larval development and life history traits. overall, we foun ...201424820554
field evaluation of the response of aedes albopictus (stegomyia albopicta) to three oviposition attractants and different ovitrap placements using black and clear autocidal ovitraps in a rural area of same, timor-leste.known oviposition attractants or stimulants were compared, singly and in combination, using inexpensive autocidal ovitraps designed to trap emerging adults, in a rural area of timor-leste during the dry season. in this area, the dengue vector aedes albopictus (stegomyia albopicta) skuse (diptera: culicidae) was abundant, but aedes aegypti (stegomyia aegypti) l. was not detected. the attractants were: (a) a compound found in aedes eggs (dodecanoic acid); (b) components of nitrogen, phosphorous an ...201424805793
chemical composition and larvicidal activity of plant extracts from clausena dentata (willd) (rutaceae) against dengue, malaria, and filariasis vectors.mosquitoes in the larval stage are attractive targets for pesticides because mosquitoes breed in water, and thus, it is easy to deal with them in this habitat. the use of conventional pesticides in the water sources, however, introduces many risks to people and/or the environment. natural pesticides, especially those derived from plants, are more promising in this aspect. aromatic plants and their essential oils are very important sources of many compounds that are used in different respects. in ...201424802866
assessing the efficacy of candidate mosquito repellents against the background of an attractive source that mimics a human host.mosquito repellents are used around the globe to protect against nuisance biting and disease-transmitting mosquitoes. recently, there has been renewed interest in the development of repellents as tools to control the transmission of mosquito-borne diseases. we present a new bioassay for the accurate assessment of candidate repellent compounds, using a synthetic odour that mimics the odour blend released by human skin. using deet (n,n-diethyl-meta-toluamide) and pmd (p-menthane-3,8-diol) as refer ...201424797537
multi-scale analysis of the associations among egg, larval and pupal surveys and the presence and abundance of adult female aedes aegypti (stegomyia aegypti) in the city of merida, mexico.despite decades of research, there is still no agreement on which indices of aedes aegypti (stegomyia aegypti) (diptera: culicidae) presence and abundance better quantify entomological risk for dengue. this study reports the results of a multi-scale, cross-sectional entomological survey carried out in 1160 households in the city of merida, mexico to establish: (a) the correlation between levels of ae. aegypti presence and abundance detected with aspirators and ovitraps; (b) which immature and eg ...201424797405
spatio-temporal dynamics of dengue 2009 outbreak in córdoba city, argentina.during 2009 the biggest dengue epidemic to date occurred in argentina, affecting almost half the country. we studied the spatio-temporal dynamics of the outbreak in the second most populated city of the country, córdoba city. confirmed cases and the results of an aedes aegypti monitoring during the outbreak were geolocated. the imported cases began in january, and the autochthonous in march. thirty-three percent of the 130 confirmed cases were imported, and occurred mainly at the center of the c ...201424795212
[dengue-related knowledge, attitudes, and practices in primary schools in tapachula, chiapas, mexico].to identify dengue-related knowledge, attitudes, and practices among primary school students in tapachula, chiapas, mexico, before and after an educational intervention.201424793869
essential oils with insecticidal activity against larvae of aedes aegypti (diptera: culicidae).insecticidal activity of the essential oils (eos) isolated from tagetes lucida, lippia alba, lippia origanoides, eucalyptus citriodora, cymbopogon citratus, cymbopogon flexuosus, citrus sinensis, swinglea glutinosa, and cananga odorata aromatic plants, grown in colombia (bucaramanga, santander), and of a mixture of l. alba and l. origanoides eos were evaluated on aedes (stegomyia) aegypti rockefeller larvae. the eos were extracted by microwave-assisted hydrodistillation and characterized by gas ...201424781026
mosquito larvicidal properties of silver nanoparticles synthesized using heliotropium indicum (boraginaceae) against aedes aegypti, anopheles stephensi, and culex quinquefasciatus (diptera: culicidae).mosquitoes transmit dreadful diseases to human beings wherein biological control of these vectors using plant-derived molecules would be an alternative to reduce mosquito population. in the present study activity of aqueous leaf extract and silver nanoparticles (agnps) synthesized using helitropium indicum plant leaves against late third instar larvae of aedes aegypti, anopheles stephensi and culex quinquefasciatus. the range of varying concentrations of synthesized agnps (8, 16, 24, 32, and 40  ...201424770671
myotropic effects of fmrfamide containing peptides on the heart of the mosquito anopheles gambiae.fmrfamide-like peptides (flps) are produced by invertebrate and vertebrate animals, and regulate diverse physiological processes. in insects, several flps modulate heart physiology, with some increasing and others decreasing dorsal vessel contraction dynamics. here, we describe the fmrfamide gene structure in the mosquito, anopheles gambiae, quantify the developmental and spatial expression of fmrfamide and its putative receptor (fmrfamider), and show that the peptides fmrfamide and saldknfmrfam ...201424747482
cytotoxicity of piperamides towards aedes aegypti (diptera: culicidae).the effectiveness of the amides piplartine and piperlonguminine isolated from piper species for controlling l3 and l4 of aedes aegypti (l.) was assessed through bioassays at concentrations ranging from 1 to 300 g/l ml. piplartine reduced the mosquito development period and caused larval mortality only at concentrations > 100 microg/ml, whereas piperlonguminine resulted in an extended period of mosquito development (10 microg/ml) and caused 100% larval mortality (30 microg/ml) within 24 h. the to ...201424724297
sublethal effect of pyriproxyfen released from a fumigant formulation on fecundity, fertility, and ovicidal action in aedes aegypti (diptera: culicidae).dengue and dengue hemorrhagic fever are mosquito-borne viral diseases that coincide with the distribution of aedes aegypti (l.), the primary vector in the tropical and semitropical world. with no available vaccine, controlling the dengue vector is essential to prevent epidemics. the effects of the insect growth regulator pyriproxyfen on ae. aegypti adults that survived a treatment with a sublethal dose were investigated in the laboratory, including effects on their reproductive potential. pyripr ...201424724294
evaluation of the effectiveness of mass trapping with bg-sentinel traps for dengue vector control: a cluster randomized controlled trial in manaus, brazil.the objective of this study was to assess the effectiveness of bg-sentinel (bgs) traps for mass trapping at the household level to control the dengue vector, aedes aegypti (l.), in manaus (brazil) by performing a cluster randomized controlled trial. after an initial questionnaire and baseline monitoring, 6 out of 12 clusters were randomly allocated to the intervention arm, where participating premises received one bgs trap for mass trapping. the other six clusters did not receive traps and were ...201424724291
performance of the plant-based repellent tt-4302 against mosquitoes in the laboratory and field and comparative efficacy to 16 mosquito repellents against aedes aegypti (diptera: culicidae).repellent efficacy of the plant-based repellent, tt-4302 (5% geraniol), was compared with 16 other products in laboratory arm-in-cage trials against aedes aegypti (l). eight repellents (badger, bioud, burt's bees, california baby, cutter natural, ecosmart, herbal armor, and skinsmart) exhibited a mean repellency below 90% to ae. aegypti at 0.5 h after application. three repellents (buzz away extreme, cutter advanced, and off! botanicals lotion) fell below 90% repellency 1.5 h after application. ...201424724289
[evaluation of personal protective equipment used by malathion sprayers in dengue control in são paulo, brazil].malathion insecticide in vegetable oil is used to control aedes aegypti and is applied by spraying, which requires the use of personal protective equipment (ppe). the current study assessed the capacity of ppe suits to retain malathion. the study monitored field spraying in são paulo, brazil. before each spraying, feminine sanitary napkins were placed under and upon the ppe suit in the chest, back, and forearms. after spraying, the sanitary napkins were removed, labeled, and submitted to extract ...201424714946
phylogenetic analysis of the gst family in anopheles (nyssorhynchus) darlingi.anopheles darlingi root, 1926 and anopheles gambiae (diptera: culicidae) are the most important human malaria vectors in south america and africa, respectively. the two species are estimated to have diverged 100 million years ago. studies on the phylogenetics and evolution of gene sequences, such as glutathione s-transferase (gst) in disease-transmitting mosquitoes are scarce. the sigma class gst (kc890767) from the transcriptome of an. darlingi captured in the brazilian amazon was studied by in ...201424713199
design, synthesis, acetylcholinesterase inhibition and larvicidal activity of girgensohnine analogs on aedes aegypti, vector of dengue fever.girgensohnine alkaloid was used as a natural model in the design and generation of new alkaloid-like α-aminonitrile series that was completed by the use of ssa-catalyzed strecker reaction between commercial and inexpensive substituted benzaldehydes, piperidine (pyrrolidine, morpholine and n-methylpiperazine) and acetone cyanohydrin. calculated admetox parameters of the designed analogs revealed their good pharmacokinetic profiles indicating lipophilic characteristics. in vitro ache enzyme test s ...201424704612
homodimerization propensity of the intrinsically disordered n-terminal domain of ultraspiracle from aedes aegypti.the mosquito aedes aegypti is the principal vector of dengue, one of the most devastating arthropod-borne viral infections in humans. the isoform specific a/b region, called the n-terminal domain (ntd), is hypervariable in sequence and length and is poorly conserved within the ultraspiracle (usp) family. the usp protein together with ecdysteroid receptor (ecr) forms a heterodimeric complex. up until now, there has been little data on the molecular properties of the isolated usp-ntd. here, we des ...201424704038
larvicidal potential of wild mustard (cleome viscosa) and gokhru (tribulus terrestris) against mosquito vectors in the semi-arid region of western rajasthan.cleome viscosa l. (family: capparaceae) commonly known as tickweed or wild mustard and tribulus terrestris l. (family: zygophyllaceae) commonly known as gokhru, growing wildly in the desert areas in the monsoon and post monsoon season, are of great medicinal importance. comparative larvicidal efficacy of the extracts from seeds of c. viscosa and fruits and leaves of t. terrestris was evaluated against 3rd or early 4th stage larvae of anopheles stephensi (liston), aedes aegypti (linnaeus) and cul ...201424665757
aedes aegypti juvenile hormone acid methyl transferase, the ultimate enzyme in the biosynthetic pathway of juvenile hormone iii, exhibits substrate control.we report on the cloning, sequencing, characterization, 3d modeling and docking of aedes aegypti juvenile hormone acid methyl transferase (aeajhamt), the enzyme that converts juvenile hormone acid (jha) into juvenile hormone (jh). purified recombinant aeajhamt was extensively characterized for enzymatic activity and the michaelis menten kinetic parameters km, vmax, k(cat) (turn over number) and k(cat)/km (catalytic efficiency) using jha and its analogues as substrates. aeajhamt methylates jha ii ...201424657668
identification of life-stage and tissue-specific splice variants of an inward rectifying potassium (kir) channel in the yellow fever mosquito aedes aegypti.inward-rectifier potassium (kir) channels play key roles in nerve, muscle, and epithelial cells in mammals, but their physiological roles in insects remain to be determined. the yellow fever mosquito (aedes aegypti) possesses five different genes encoding kir channel subunits: kir1, kir2a, kir2b, kir2b', and kir3. we have recently cloned and characterized the kir1, kir2b, and kir3 cdnas in the renal (malpighian) tubules of adult female ae. aegypti. here we characterize the expression of the kir2 ...201424657620
[genetic differences between populations of aedes aegypti from municipalities in northern colombia, with high and low dengue incidence].aedes aegypti is the principal vector of dengue in urban areas. despite its epidemiological importance, the genetic variability of colombian populations of this species is unknown.201324652253
[determining the status of susceptibility to organophosphate, carbamate and pyrethroids insecticides in populations of aedes aegypti linneaus, 1762 (diptera: culicidae) in panamá].we studied the susceptibility to organophosphate, carbamate and pyrethroid insecticides of aedes aegypti from different regions of high transmission risk for dengue in panama.201324652251
low-cost and eco-friendly green synthesis of silver nanoparticles using feronia elephantum (rutaceae) against culex quinquefasciatus, anopheles stephensi, and aedes aegypti (diptera: culicidae).mosquitoes transmit serious human diseases, causing millions of deaths every year. the use of synthetic insecticides to control vector mosquitoes has caused physiological resistance and adverse environmental effects in addition to high operational cost. insecticides of synthesized natural products for vector control have been a priority in this area. in the present study, the larvicidal activity of silver nanoparticles (agnps) synthesized using feronia elephantum plant leaf extract against late ...201424647984
macroclimate determines the global range limit of aedes aegypti.aedes aegypti is the main vector of dengue and a number of other diseases worldwide. because of the domestic nature of this mosquito, the relative importance of macroclimate in shaping its distribution has been a controversial issue. we have captured here the worldwide macroclimatic conditions occupied by a. aegypti in the last century. we assessed the ability of this information to predict the species' observed distribution using supra-continental spatially-uncorrelated data. we further project ...201424643859
temporal genetic structure of major dengue vector aedes aegypti from manaus, amazonas, brazil.in recent years, high levels of aedes aegypti infestation and several dengue outbreaks with fatal outcome cases have been reported in manaus, state of amazonas, brazil. this situation made it important to understand the genetic structure and gene flow patterns among the populations of this vector in manaus, vital pieces of information for their management and development of new control strategies. in this study, we used nine microsatellite loci to examine the effect of seasonality on the genetic ...201424631342
[design and evaluation of a ovitrap for monitoring and control of aedes aegypti, dengue fever vector].to design and evaluate in field conditions an ovitrap with chemical attractants and a population regulator for the monitoring and control of dengue vectors.201424626622
the maxillary palp of aedes aegypti, a model of multisensory integration.female yellow-fever mosquitoes, aedes aegypti, are obligate blood-feeders and vectors of the pathogens that cause dengue fever, yellow fever and chikungunya. this feeding behavior concludes a series of multisensory events guiding the mosquito to its host from a distance. the antennae and maxillary palps play a major role in host detection and other sensory-mediated behaviors. compared to the antennae, the maxillary palps are a relatively simple organ and thus an attractive model for exploration ...201424613607
development of the gravid aedes trap for the capture of adult female container-exploiting mosquitoes (diptera: culicidae).monitoring dengue vector control by sampling adult aedes aegypti (l.) recently has been used to replace both larval and pupal surveys. we have developed and evaluated the gravid aedes trap (gat) through a sequential behavioral study. the gat does not require electricity to function, and trapped mosquitoes are identified easily during trap inspections. the gat concept relies on visual and olfactory cues to lure gravid ae. aegypti and an insecticide to kill trapped mosquitoes. gravid mosquitoes ar ...201424605470
evaluation of moringa oleifera seed lectin in traps for the capture of aedes aegypti eggs and adults under semi-field conditions.the water-soluble lectin isolated from moringa oleifera seeds (wsmol) is a larvicidal, ovicidal, and oviposition-stimulating agent against aedes aegypti under laboratory conditions. this study investigated the effect of wsmol in traps for the capture of a. aegypti eggs and adult females under semi-field conditions and determined whether gravid females could detect wsmol by an olfactory response. wsmol was isolated according to a previously described procedure using chitin chromatography. the bio ...201424604386
knockdown and repellent effect of permethrin-impregnated army uniform cloth against aedes aegypti after different cycles of washings.personnel protection is one of the methods for protection from bites of mosquitoes and other arthropod vectors transmitting many dreadful diseases. insect repellents and other plant products are normally used to ward off mosquitoes. application of synthetic pyrethroid permethrin on cloth is adopted for repelling arthropod vectors in many countries for military and civil purposes. in the present study, attempt has been made to impregnate permethrin in the army uniform cloth and to evaluate for it ...201424595642
the genetics of chemoreception in the labella and tarsi of aedes aegypti.the yellow-fever mosquito aedes aegypti is a major vector of human diseases, such as dengue, yellow fever, chikungunya and west nile viruses. chemoreceptor organs on the labella and tarsi are involved in human host evaluation and thus serve as potential foci for the disruption of blood feeding behavior. in addition to host detection, these contact chemoreceptors mediate feeding, oviposition and conspecific recognition; however, the molecular landscape of chemoreception in these tissues remains m ...201424582661
entomological surveillance, spatial distribution, and diversity of culicidae (diptera) immatures in a rural area of the atlantic forest biome, state of são paulo, brazil.because of the high adaptive capacity of mosquitoes, studies that focus on transitional environments become very important, such as those in rural areas, which are considered as bridges between wild diseases and human populations of urban areas. in this study, a survey of the existing species of mosquitoes was performed in an atlantic forest area of the city of santa bárbara d'oeste, são paulo state, brazil, using traps for immatures and analyzing the frequency and distribution of these insects ...201324581361
a new tent trap for monitoring the daily activity of aedes aegypti and aedes albopictus.in this study, we designed a new tent trap; the biodivector (bdv) tent trap, consisting of two rectangular tents that use human bait without endangering the technical personnel. the daily activity pattern of aedes aegypti and aedes albopictus in intra, peri, and extradomiciliary sites was studied in an endemic area of dengue in southern mexico by using the bdv tent trap. totals of 3,128 individuals of ae. aegypti and 833 ae. albopictus were captured. more ae. aegypti males than females were caug ...201324581356
aedes species in treeholes and fruit husks between dry and wet seasons in southeastern senegal.during the dry season in february, 2010 and the wet season in september, 2011 we sampled mosquito larvae and eggs from treeholes of seven native hardwood species and the husks of saba senegalensis in 18 sites in the pk-10 forest in southeastern senegal. larvae were reared to adults for species identification. in the dry season, we recovered 408 aedes mosquitoes belonging to seven species. aedes aegypti s.l. comprised 42.4% of the collection, followed by ae. unilineatus (39%). in contrast to repo ...201324581351
larvicidal, ovicidal, and oviposition-deterrent activities of four plant extracts against three mosquito species.in mosquito control programs, insecticides of botanical origin have the potential to eliminate eggs, larvae, and adults. so, the larvicidal, ovicidal, and oviposition-deterrent activities of petroleum ether and ethyl acetate extracts of the leaves of eugenia jambolana, solidago canadensis, euodia ridleyi, and spilanthes mauritiana were assayed against the three vector mosquito species, namely anopheles stephensi, aedes aegypti, and culex quinquefasciatus. the larval bioassay was conducted follow ...201424562451
an unusual cause of acute abdominal pain in dengue fever.dengue fever is an acute febrile viral disease caused by the bite of aedes aegypti mosquito. it is a major health problem especially in tropical and subtropical areas including south east asia and pakistan. in the past few years, dengue fever has been endemic in northern punjab. physicians managing dengue fever come across varied and uncommon complications of dengue fever. we report a case of dengue fever that developed severe right upper quadrant abdominal pain and induration after extreme retc ...201424560393
efficacy and safety of topical trikatu preparation in, relieving mosquito bite reactions: a randomized controlled trial.trikatu is composed of dried fruits of piper nigrum l and piper retrofractum vahl, and dried rhizomes of zingiber officinale r. although this preparation has been used to relieve pruritis, pain, and inflammation for a long time, there is no clinical evidence to confirm its efficacy and safety. therefore, we performed a double-blind, within person-randomized controlled study of 30 healthy volunteers to determine efficacy and safety of topical trikatu on mosquito bite reactions.201424559814
ecotoxicity and environmental risk assessment of larvicides used in the control of aedes aegypti to daphnia magna (crustacea, cladocera).dengue transmitted by mosquitoes of the genus aedes, species aegypti, is a major public health concern in brazil. the chemical control of the mosquito larvae has been performed with the larvicide temephos since 1967. however, vector resistance was reported to temephos in several brazilian states, and the ministry of health ordered the replacement of this larvicide by diflubenzuron (dfb), an inhibitor of chitin synthesis. both insecticides are diluted in water with larvae and are able to reach aq ...201424555645
larvicidal potential of silver nanoparticles synthesized from leucas aspera leaf extracts against dengue vector aedes aegypti.vector-borne diseases caused by mosquitoes are one of the major economic and health problems in many countries. the aedes aegypti mosquito is a vector of several diseases in humans like yellow fever and dengue. vector control methods involving the use of chemical insecticides are becoming less effective due to development of insecticides resistance, biological magnification of toxic substances through the food chain, and adverse effects on environmental quality and non-target organisms including ...201424553980
first record and demonstration of a southward expansion of aedes albopictus into orange walk town, belize, central america.the first record of aedes albopictus in northern belize was made in orange walk town, orange walk district, on november 3, 2011. aedes spp. larvae were collected during a routine ministry of health mosquito survey and reared to adults. upon emergence, a mixed population of aedes aegypti (35) and ae. albopictus (11) was observed (aegypti:albopictus = 3:1). subsequent larval and adult surveys in orange walk and corozal district, also in northern belize, have confirmed the presence of ae. albopictu ...201324551972
methoprene effects on survival and reproductive performance of adult female and male aedes aegypti.methoprene is a juvenile hormone analog commonly used for the control of mosquito larvae. it acts through interference with normal metamorphosis, resulting in mortality prior to and during adult emergence. methoprene is not commonly used for the control of adult mosquitoes, due to an absence of acute effects. here, we have evaluated for chronic effects caused by the exposure of adult aedes aegypti mosquitoes to methoprene. we applied methoprene to adults, both through 1) topical application to t ...201324551970
the risk of aedes aegypti breeding and premises condition in south mexico.a recent innovation instrumented for the dengue prevention and control program in mexico is the use of the premises condition index (pci) as an indicator of risk for the vector aedes aegypti infestation in dengue-endemic localities of mexico. this paper addresses whether further improvements for the dengue control program could be made if the prevalence and productivity of ae. aegypti populations could be reliably predicted using pci at the household level, as well as medium-sized neighborhoods. ...201324551966
vertical infestation of the dengue vectors aedes aegypti and aedes albopictus in apartments in kuala lumpur, malaysia.dengue is a serious public health problem in malaysia. the aim of this study was to compare the vertical infestation of aedes population in 2 apartments in kuala lumpur with different status of dengue incidence (i.e., high-dengue-incidence area and area with no reported dengue cases). the study was also conducted to assess the relationship between environmental factors such as rainfall, temperature, and humidity and aedes population that may influence aedes infestation. surveillance with a mosqu ...201324551965
serosal cuticle formation and distinct degrees of desiccation resistance in embryos of the mosquito vectors aedes aegypti, anopheles aquasalis and culex quinquefasciatus.given their medical importance, mosquitoes have been studied as vectors of parasites since the late 1800's. however, there are still many gaps concerning some aspects of their biology, such as embryogenesis. the embryonic desiccation resistance (edr), already described in aedes and anopheles gambiae mosquitoes, is a peculiar trait. freshly laid eggs are susceptible to water loss, a condition that can impair their viability. edr is acquired during embryogenesis through the formation of the serosa ...201424534672
growth and development of aedes aegypti larvae at limiting food concentrations.mosquitoes have a complex life-cycle with dramatic changes in shape, function, and habitat. aedes aegypti was studied by growing individual larvae at different concentrations of a defined rich food source. at higher food concentrations, rate of larval growth was faster, but the time required for 4th instar larvae to molt into the pupal stage was unexpectedly extended. these opposite tendencies resulted in constant times from hatching to pupation and up to adult eclosion at permissive food concen ...201424524949
oviposition and olfaction responses of aedes aegypti mosquitoes to insecticides.insecticide applications are not particularly effective on aedes aegypti mosquitoes which has been attributed to their 'closet' behaviour, or ability to rest in places that remain unexposed to insecticides. some researchers have suggested that insecticides repel mosquitoes, which would result in less exposure and increased dispersal. if repellence due to insecticides is a fact, acquiring a vector-borne disease, such as dengue, could legitimately be attributed to local vector control efforts and ...201324522139
relative developmental and reproductive fitness associated with f1534c homozygous knockdown resistant gene in aedes aegypti from thailand.the effect of permethrin resistance, conferred by a homozygous mutation (f1534c) in the voltage-gated sodium channel protein, upon the reproductive fitness of aedes aegypti (pmd-r strain) from thailand was evaluated by comparing with a pyrethroid-susceptible sub colony (pmd strain). the parameters evaluated included larval development time, pupation success, adult emergence, egg production and hatchability, mating ability, female wing length and adult longevity. larval development times were sim ...201324522132
release of thiotepa sterilized males into caged populations of aedes aegypti: life table analysis.successful sit trials against mosquitoes in the 1960-70s were achieved by sterilizing male mosquitoes using chemosterilants. their use was discontinued after concerns were raised about the effect of residues on non-target organisms, although scant evidence has been published. irradiation is an expensive process; chemosterilization could be an affordable option for implementing sit programs in developing countries. we compare life table parameters of three aedes aegypti populations comprising dif ...201424513037
aedes aegypti midgut remodeling during metamorphosis.the aedes aegypti midgut is restructured during metamorphosis; its epithelium is renewed by replacing the digestive and endocrine cells through stem or regenerative cell differentiation. shortly after pupation (white pupae) begins, the larval digestive cells are histolized and show signs of degeneration, such as autophagic vacuoles and disintegrating microvilli. simultaneously, differentiating cells derived from larval stem cells form an electron-dense layer that is visible 24h after pupation be ...201424472855
recent and future environmental suitability to dengue fever in brazil using species distribution model.dengue fever is a mosquito-borne disease that affects more than 2.5 billion people worldwide. here, we used the dataset of municipality infestation level from the brazilian health ministry with the aim of building vector distribution models to identify epidemiological hotspots.201424463584
effect of housing factors on infestation by aedes aegypti (l.) and aedes albopictus skuse in urban hanoi city, vietnam.to determine the effect of housing factors on infestation with aedes aegypti (l.) and aedes albopictus skuse we conducted an entomological survey and inspection of 267 urban houses in hanoi city, vietnam. two hundred ten pupae and 194 adult ae. aegypti were collected from 19 and 88 houses, respectively. one hundred eighty-one pupae and 24 adult ae. albopictus were collected from 21 and 14 houses, respectively. the presence of a private well was associated with increasing infestation with ae. aeg ...201324450235
effects of grapefruit (citrus paradisi macf) (rutaceae) peel oil against developmental stages of aedes aegypti (diptera: culicidae).laboratory bioassay of the essential oil extracted from the grapefruit (citrus paradisi) peel by steam distillation was carried out against the developmental stages of the yellow fever vector aedes aegypti to evaluate its toxicity, and ovicidal and larvicidal potency. volatile oil components isolated and characterized by coupled gas chromatography/mass spectrometry included varying levels of monoterpene aldehydes, alcohols, and esters. test results of the essential oil showed that egg hatching w ...201324450234
identification and expression profile analysis of putative odorant-binding proteins in sitodiplosis mosellana (gehin) (diptera: cecidomyiidae).odorant binding proteins (obps) contribute to the remarkable sensitivity of the insect's olfactory system and play important roles in the olfactory recognition. the orange blossom midge, sitodiplosis mosellana is a cereal specialist, and utilizes pheromone and host odorant as a cue for its mating and oviposition. however, obp genes have not been largely identified in s. mosellana. based on the sequenced transcriptome database, twenty-six obp genes were identified in s. mosellana for the first ti ...201424440700
larvicidal and pupicidal activities of essential oils from zingiberaceae plants against aedes aegypti (linn.) and culex quinquefasciatus say mosquitoes.we conducted this study to investigate the efficacy of herbal essential oils from 12 species of zingiberaceae plants to determine their larvicidal and pupicidal activity against fourth instar larvae and pupae of aedes aegypti and culex quinquefasciatus mosquitoes. probit analysis was used to analyze the data. larval mortality was recorded at 1, 5, 10, 15, 30 and 60 minutes and 24 hours. pupal mortality was recorded at 15 and 30 minutes and 1, 3, 6, 12, 24 and 48 hours. all the essential oils tes ...201324437311
comparative field efficacy of newly developed formulations of larvicides against aedes aegypti (l.) (diptera: culicidae).aedes aegypti (l.) is known as vector of dengue and chikungunya fever. larvicides are used to control this vector. we evaluated the efficacy of newly developed formulations of larvicides to control ae. aegypti under field conditions for 24 weeks post single application. mosdop p and mosdop tb containing diflubenzuron (2% and 40 mg/tablet, respectively) as the active ingredient, were applied at a dosage of 0.1 mg a.i./1 and mosquit tb10, mosquit tb100 and temecal containing temephos (1%, 10% and ...201324437310
transport of h(+), na(+) and k(+) across the posterior midgut of blood-fed mosquitoes (aedes aegypti).following ingestion of a blood meal, the adult female mosquito undergoes a massive diuresis during which na(+), cl(-) and water are secreted at high rates by the malpighian tubules. in the hours following completion of diuresis, digestion of the k(+)-rich blood cells provides a source of energy as well as amino acids for proteins in the developing eggs. although the transport of inorganic ions by the malpighian tubules of blood-fed mosquitoes has been extensively characterized, relatively little ...201424406662
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