Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| vector competence of the aedes aegypti population from santiago island, cape verde, to different serotypes of dengue virus. | dengue is an arboviral disease caused by dengue virus (denv), whose main vectors are the mosquitoes aedes aegypti and aedes albopictus. a. aegypti is the only denv vector in cape verde, an african country that suffered its first outbreak of dengue in 2009. however, little is known about the variation in the level of vector competence of this mosquito population to the different denv serotypes. this study aimed to evaluate the vector competence of a. aegypti from the island of santiago, cape verd ... | 2015 | 25888847 |
| evaluation of a protocol for remote identification of mosquito vector species reveals bg-sentinel trap as an efficient tool for anopheles gambiae outdoor collection in burkina faso. | feasibility and costs of monitoring efforts aimed to monitor mosquito species are strictly dependent on the presence of skilled entomologists directly in the field. however, in several contexts this is not possible or easy to organize, thus limiting the possibility to obtain crucial information on presence/abundance of potential disease vectors and of new invasive species. digital imaging approaches could be extremely useful in the frame of medical entomology to overcome this limit. this work de ... | 2015 | 25888896 |
| transcriptomic insights on the abc transporter gene family in the salmon louse caligus rogercresseyi. | atp-binding cassette (abc) protein family encode for membrane proteins involved in the transport of various biomolecules through the cellular membrane. these proteins have been identified in all taxa and present important physiological functions, including the process of insecticide detoxification in arthropods. for that reason the ectoparasite caligus rogercresseyi represents a model species for understanding the molecular underpinnings involved in insecticide drug resistance. | 2015 | 25889038 |
| modelling sterile insect technique to control the population of anopheles gambiae. | there is a renewed effort to develop novel malaria control strategies as even well-implemented existing malaria control tools may fail to block transmission in some regions. currently, transgenic implementations of the sterile insect technique (sit) such as the release of insects with a dominant lethal, homing endonuclease genes, or flightless mosquitoes are in development. these implementations involve the release of transgenic male mosquitoes whose matings with wild females produce either no v ... | 2015 | 25889145 |
| utility of mosquito surveillance data for spatial prioritization of vector control against dengue viruses in three brazilian cities. | vector control remains the primary defense against dengue fever. its success relies on the assumption that vector density is related to disease transmission. two operational issues include the amount by which mosquito density should be reduced to minimize transmission and the spatio-temporal allotment of resources needed to reduce mosquito density in a cost-effective manner. recently, a novel technology, mi-dengue, was implemented city-wide in several brazilian cities to provide real-time mosqui ... | 2015 | 25889533 |
| impacts of the creation, expansion and management of english wetlands on mosquito presence and abundance - developing strategies for future disease mitigation. | the incidence of mosquito-borne diseases is increasing in europe, partly due to the incursion of a number of invasive species known to be vectors of dengue and chikungunya viruses, but also due to the involvement of native species in the transmission of west nile virus and malaria. for some of these pathogens, there is a risk of the re-emergence of vector-borne diseases that were once widespread in europe, but declined partly due to large-scale land-drainage projects. some mosquito species explo ... | 2015 | 25889666 |
| novel mutations on the ace-1 gene of the malaria vector anopheles albimanus provide evidence for balancing selection in an area of high insecticide resistance in peru. | resistance to multiple classes of insecticides has been detected in the malaria vector anopheles albimanus in northwest peru. acetylcholinesterase (ache) insensitivity has previously been associated with resistance to organophosphate (op) and carbamate (ca) insecticides in arthropods. a single point mutation on the ace-1 gene (g119s) associated with resistance to ops and cas has been described previously in four anopheline species, but not in field-collected an. albimanus. the present study aime ... | 2015 | 25889700 |
| frizzled 2 is a key component in the regulation of tor signaling-mediated egg production in the mosquito aedes aegypti. | the wnt signaling pathway was first discovered as a key event in embryonic development and cell polarity in drosophila. recently, several reports have shown that wnt stimulates translation and cell growth by activating the mtor pathway in mammals. previous studies have demonstrated that the target of rapamycin (tor) pathway plays an important role in mosquito vitellogenesis. however, the interactions between these two pathways are poorly understood in the mosquito. in this study, we hypothesized ... | 2015 | 25890109 |
| wing sexual dimorphism of pathogen-vector culicids. | sexual dimorphism in animals has been studied from different perspectives for decades. in 1874 darwin hypothesized that it was related to sexual selection, and even after nearly 140 years, when additional empirical data has become available and the subject has been investigated from a contemporary viewpoint, this idea is still supported. although mosquito (culicidae) wings are of great importance as they play a sex-specific role, little is known about wing sexual dimorphism in these pathogen-vec ... | 2015 | 25890192 |
| density of aedes aegypti and aedes albopictus and its association with number of residents and meteorological variables in the home environment of dengue endemic area, são paulo, brazil. | measure the populations of ae. aegypti and ae. albopictus adults according to sex and location inside or outside the residence, estimate ae. aegypti female density per house and per resident, and test the association with abiotic factors. | 2015 | 25890384 |
| managing dengue fever in primary care: a practical approach. | dengue is a common cause of illness seen in primary care in the tropical and subtropical countries. an understanding of the course of disease progression, risk factors, recognition of the warning signs and look out for clinical problems during the different phases of the disease will enable primary care physicians to manage dengue fever in an appropriate and timely manner to reduce morbidity and mortality. | 2014 | 25893065 |
| cloning and expressing a highly functional and substrate specific farnesoic acid o-methyltransferase from the asian citrus psyllid (diaphorina citri kuwayama). | the asian citrus psyllid, diaphorina citri, transmits a phloem-limited bacterium, candidatus 'liberibacter' asiaticus that causes citrus greening disease. because juvenile hormone (jh) plays an important role in adult and nymphal development, we studied the final steps in jh biosynthesis in d. citri. a putative jh acid methyltransferase ortholog gene (jmtd) and its cognate cdna were identified by searching d. citri genome database. expression analysis shows expression in all life stages. in adul ... | 2015 | 25893162 |
| spatio-temporal distribution of aedes aegypti (diptera: culicidae) mitochondrial lineages in cities with distinct dengue incidence rates suggests complex population dynamics of the dengue vector in colombia. | aedes aegypti is the primary vector of the four serotypes of dengue virus (denv1-4), chikungunya and yellow fever virus to humans. previous population genetic studies have revealed a particular genetic structure among the vector populations in the americas that suggests differences in the ability to transmit denv. in colombia, despite its high epidemiologic importance, the genetic population structure and the phylogeographic depiction of ae. aegypti, as well as its relationship with the epidemio ... | 2015 | 25893246 |
| male seminal fluid substances affect sperm competition success and female reproductive behavior in a seed beetle. | male seminal fluid proteins are known to affect female reproductive behavior and physiology by reducing mating receptivity and by increasing egg production rates. such substances are also though to increase the competitive fertilization success of males, but the empirical foundation for this tenet is restricted. here, we examined the effects of injections of size-fractioned protein extracts from male reproductive organs on both male competitive fertilization success (i.e., p2 in double mating ex ... | 2015 | 25893888 |
| tracing the evolutionary origins of insect renal function. | knowledge on neuropeptide receptor systems is integral to understanding animal physiology. yet, obtaining general insight into neuropeptide signalling in a clade as biodiverse as the insects is problematic. here we apply fluorescent analogues of three key insect neuropeptides to map renal tissue architecture across systematically chosen representatives of the major insect orders, to provide an unprecedented overview of insect renal function and control. in endopterygote insects, such as drosophi ... | 2015 | 25896425 |
| the deadly ebola threat in the midst of an overwhelming dengue epidemic. | the recent death tolls and morbidities associated with two deadly viral haemorrhagic fevers (vhfs), i.e., ebola and dengue, are simply shocking. by the end of august 2014, 65 672 people were afflicted with dengue fever (df) in malaysia, with 9505 from kelantan, and there were 128 reported deaths. more astounding are the death tolls associated with ebola: 3091 deaths from 6574 reported cases so far. it is not difficult to imagine the potential disaster if ebola spreads beyond africa. vhfs are cha ... | 0 | 25897277 |
| global transcriptional dynamics of diapause induction in non-blood-fed and blood-fed aedes albopictus. | aedes albopictus is a vector of increasing public health concern due to its rapid global range expansion and ability to transmit dengue virus, chikungunya virus and a wide range of additional arboviruses. traditional vector control strategies have been largely ineffective against ae. albopictus and novel approaches are urgently needed. photoperiodic diapause is a crucial ecological adaptation in a wide range of temperate insects. therefore, targeting the molecular regulation of photoperiodic dia ... | 2015 | 25897664 |
| allatotropin modulates myostimulatory and cardioacceleratory activities in rhodnius prolixus (stal). | haematophagous insects can ingest large quantities of blood in a single meal and eliminate high volumes of urine in the next few hours. this rise in diuresis is possible because the excretory activity of the malpighian tubules is facilitated by an increase in haemolymph circulation as a result of intensification of aorta contractions combined with an increase of the anterior midgut peristaltic waves. it has been previously described that haemolymph circulation during post-prandial diuresis is st ... | 2015 | 25897783 |
| chikungunya virus outbreak, dominica, 2014. | 0 | 25898214 | |
| east/central/south african genotype chikungunya virus, brazil, 2014. | 0 | 25898939 | |
| decrease of larval and subsequent adult anopheles sergentii populations following feeding of adult mosquitoes from bacillus sphaericus-containing attractive sugar baits. | bacillus sphaericus is a mosquito-larvae pathogen which proliferates in the host cadavers, spreading and preserving the infection within the larval habitats for prolonged periods. in this pilot field study, we presented b. sphaericus-containing attractive sugar baits (asb) to wild anopheles sergentii adults, with the assumption that bait-fed, b. sphaericus-carrying mosquitoes are able to efficiently transmit the pathogen to the larval habitats, causing larval mortality and leading to a decrease ... | 2015 | 25899788 |
| heritability of attractiveness to mosquitoes. | female mosquitoes display preferences for certain individuals over others, which is determined by differences in volatile chemicals produced by the human body and detected by mosquitoes. body odour can be controlled genetically but the existence of a genetic basis for differential attraction to insects has never been formally demonstrated. this study investigated heritability of attractiveness to mosquitoes by evaluating the response of aedes aegypti (=stegomyia aegypti) mosquitoes to odours fro ... | 2015 | 25901606 |
| susceptibility to temephos, permethrin and deltamethrin of aedes aegypti (diptera: culicidae) from muang district, phitsanulok province, thailand. | to investigate the susceptibility to temephos, permethrin and deltamethrin of aedes aegypti (ae. aegypti), collected from areas with high incidence of dengue hemorrhagic fever cases in phitsanulok province, thailand. | 2015 | 25901918 |
| long non-coding rna discovery across the genus anopheles reveals conserved secondary structures within and beyond the gambiae complex. | long non-coding rnas (lncrnas) have been defined as mrna-like transcripts longer than 200 nucleotides that lack significant protein-coding potential, and many of them constitute scaffolds for ribonucleoprotein complexes with critical roles in epigenetic regulation. various lncrnas have been implicated in the modulation of chromatin structure, transcriptional and post-transcriptional gene regulation, and regulation of genomic stability in mammals, caenorhabditis elegans, and drosophila melanogast ... | 2015 | 25903279 |
| effect of repeat human blood feeding on wolbachia density and dengue virus infection in aedes aegypti. | the introduction of the endosymbiotic bacterium, wolbachia into aedes aegypti populations is a novel approach to reduce disease transmission. the presence of wolbachia limits the ability of the mosquito to transmit dengue virus (denv) and the strength of this effect appears to correlate with wolbachia densities in the mosquito. there is also some evidence that wolbachia densities may increase following the consumption of a bloodmeal. here we have examined whether multiple blood feeds lead to inc ... | 2015 | 25903749 |
| can plant viruses cross the kingdom border and be pathogenic to humans? | phytoviruses are highly prevalent in plants worldwide, including vegetables and fruits. humans, and more generally animals, are exposed daily to these viruses, among which several are extremely stable. it is currently accepted that a strict separation exists between plant and vertebrate viruses regarding their host range and pathogenicity, and plant viruses are believed to infect only plants. accordingly, plant viruses are not considered to present potential pathogenicity to humans and other ver ... | 2015 | 25903834 |
| efficacy of insecticide treated nets against mixed population of mosquitoes at dayalbagh-agra. | 0 | 25905088 | |
| a small antigenic determinant of the chikungunya virus e2 protein is sufficient to induce neutralizing antibodies which are partially protective in mice. | the mosquito-borne chikungunya virus (chikv) causes high fever and severe joint pain in humans. it is expected to spread in the future to europe and has recently reached the usa due to globalization, climate change and vector switch. despite this, little is known about the virus life cycle and, so far, there is no specific treatment or vaccination against chikungunya infections. we aimed here to identify small antigenic determinants of the chikv e2 protein able to induce neutralizing immune resp ... | 2015 | 25905779 |
| from lab to field: the influence of urban landscapes on the invasive potential of wolbachia in brazilian aedes aegypti mosquitoes. | the symbiotic bacterium wolbachia is currently being trialled as a biocontrol agent in several countries to reduce dengue transmission. wolbachia can invade and spread to infect all individuals within wild mosquito populations, but requires a high rate of maternal transmission, strong cytoplasmic incompatibility and low fitness costs in the host in order to do so. additionally, extensive differences in climate, field-release protocols, urbanization level and human density amongst the sites where ... | 2015 | 25905888 |
| a bayesian hierarchical model for estimation of abundance and spatial density of aedes aegypti. | strategies to minimize dengue transmission commonly rely on vector control, which aims to maintain ae. aegypti density below a theoretical threshold. mosquito abundance is traditionally estimated from mark-release-recapture (mrr) experiments, which lack proper analysis regarding accurate vector spatial distribution and population density. recently proposed strategies to control vector-borne diseases involve replacing the susceptible wild population by genetically modified individuals' refractory ... | 2015 | 25906323 |
| virulency of novel nanolarvicide from trichoderma atroviride against aedes aegypti (linn.): a clsm analysis. | aedes aegypti is the vector for transmitting dengue, chikungunya, and yellow fever. these diseases' transmission has increased predominantly in urban and semi-urban areas as a major public health concern. in present investigation, trichoderma atroviride culture filtrates were used for the synthesis of silver nanoparticle. moreover, t. atroviride is a free-living and rapidly growing fungi common in soil and root ecosystem. this fungi is an exceptionally good model for biocontrol and more signific ... | 2015 | 25907629 |
| antiviral activity of an n-allyl acridone against dengue virus. | dengue virus (denv), a member of the family flaviviridae, is at present the most widespread causative agent of a human viral disease transmitted by mosquitoes. despite the increasing incidence of this pathogen, there are no antiviral drugs or vaccines currently available for treatment or prevention. in a previous screening assay, we identified a group of n-allyl acridones as effective virus inhibitors. here, the antiviral activity and mode of action targeted to viral rna replication of one of th ... | 2015 | 25908170 |
| toll signals regulate dorsal-ventral patterning and anterior-posterior placement of the embryo in the hemipteran rhodnius prolixus. | insect embryonic dorso-ventral patterning depends greatly on two pathways: the toll pathway and the bone morphogenetic protein pathway. while the relative contribution of each pathway has been investigated in holometabolous insects, their role has not been explored in insects with a hemimetabolous type of development. the hemimetabolous insect rhodnius prolixus, an important vector of chagas disease in the americas, develops from an intermediate germ band and displays complex movements during ka ... | 2014 | 25908955 |
| small bite, big threat: the burden of vector-borne diseases. | 0 | 25909072 | |
| protobug: functional families from the complete proteomes of insects. | protobug (http://www.protobug.cs.huji.ac.il) is a database and resource of protein families in arthropod genomes. protobug platform presents the relatedness of complete proteomes from 17 insects as well as a proteome of the crustacean, daphnia pulex. the represented proteomes from insects include louse, bee, beetle, ants, flies and mosquitoes. based on an unsupervised clustering method, protein sequences were clustered into a hierarchical tree, called protobug. protobug covers about 300,000 sequ ... | 2015 | 25911153 |
| di-rhamnolipid is a mosquito pupicidal metabolite from pseudomonas fluorescens (vcrc b426). | pseudomonas fluorescens migula (vcrc b426) produces a secondary metabolite, which was found to be active against pupae of vector mosquitoes namely culex quinquefasciatus, anopheles stephensi and aedes aegypti. the mosquito pupicidal metabolite from p. fluoescens was mass produced and separated by ethyl acetate extraction and purified further by silica gel column chromatography, fplc, hplc and tlc. the purified metabolite was characterized by nmr, ft-ir, lc-ms and maldi-tof. the ft-ir, (1)h and ( ... | 2015 | 25912083 |
| a neuron-specific antiviral mechanism prevents lethal flaviviral infection of mosquitoes. | mosquitoes are natural vectors for many etiologic agents of human viral diseases. mosquito-borne flaviviruses can persistently infect the mosquito central nervous system without causing dramatic pathology or influencing the mosquito behavior and lifespan. the mechanism by which the mosquito nervous system resists flaviviral infection is still largely unknown. here we report that an aedes aegypti homologue of the neural factor hikaru genki (aahig) efficiently restricts flavivirus infection of the ... | 2015 | 25915054 |
| structures and functions of insect arylalkylamine n-acetyltransferase (iaanat); a key enzyme for physiological and behavioral switch in arthropods. | the evolution of n-acetyltransfeases (nats) seems complex. vertebrate arylalkylamine n-acetyltransferase (aanat) has been extensively studied since it leads to the synthesis of melatonin, a multifunctional neurohormone prevalent in photoreceptor cells, and is known as a chemical token of the night. melatonin also serves as a scavenger for reactive oxygen species. this is also true with invertebrates. nat therefore has distinct functional implications in circadian function, as timezymes (aanat), ... | 2015 | 25918505 |
| chemical composition and biological activity of essential oils of dracocephalum heterophyllum and hyssopus officinalis from western himalaya. | the essential oils of two representatives of the lamiaceae, dracocephalum heterophyllum benth. and hyssopus officinalis l., are described for their antifungal, antibacterial, larvicidal and inect biting deterrent activities. additionally, the chemical compositions of the essential oils, analyzed by simultaneous gc-ms and gc-fid, and odor descriptions are given. the main components of h. officinalis oil were pinocarvone, cis-pinocamphone, and β-pinene. citronellol was found as the main compound o ... | 2015 | 25920235 |
| the expression profile of aedes albopictus mirnas is altered by dengue virus serotype-2 infection. | aedes albopictus is an important vector of dengue virus (denv) and it has quickly invaded the tropical and temperate environments worldwide. a few studies have shown that, micrornas (mirnas) regulate mosquito defense against pathogens. however, there is no systematic analysis of the impact of denv infection on mirna expression in ae. albopictus. we conducted this study to investigate the mirna expression of ae. albopictus upon denv-2 infection using illumina rna sequencing. | 2015 | 25922662 |
| field evaluation of a push-pull system to reduce malaria transmission. | malaria continues to place a disease burden on millions of people throughout the tropics, especially in sub-saharan africa. although efforts to control mosquito populations and reduce human-vector contact, such as long-lasting insecticidal nets and indoor residual spraying, have led to significant decreases in malaria incidence, further progress is now threatened by the widespread development of physiological and behavioural insecticide-resistance as well as changes in the composition of vector ... | 2015 | 25923114 |
| a household serosurvey to estimate the magnitude of a dengue outbreak in mombasa, kenya, 2013. | dengue appears to be endemic in africa with a number of reported outbreaks. in february 2013, several individuals with dengue-like illnesses and negative malaria blood smears were identified in mombasa, kenya. dengue was laboratory confirmed and an investigation was conducted to estimate the magnitude of local transmission including a serologic survey to determine incident dengue virus (denv) infections. consenting household members provided serum and were questioned regarding exposures and medi ... | 2015 | 25923210 |
| native wolbachia from aedes albopictus blocks chikungunya virus infection in cellulo. | wolbachia, a widespread endosymbiont of terrestrial arthropods, can protect its host against viral and parasitic infections, a phenotype called "pathogen blocking". however, in some cases wolbachia may have no effect or even enhance pathogen infection, depending on the host-wolbachia-pathogen combination. the tiger mosquito aedes albopictus is naturally infected by two strains of wolbachia, walba and walbb, and is a competent vector for different arboviruses such as dengue virus (denv) and chiku ... | 2015 | 25923352 |
| cathepsin gene family reveals transcriptome patterns related to the infective stages of the salmon louse caligus rogercresseyi. | cathepsins are proteases involved in the ability of parasites to overcome and/or modulate host defenses so as to complete their own lifecycle. however, the mechanisms underlying this ability of cathepsins are still poorly understood. one excellent model for identifying and exploring the molecular functions of cathepsins is the marine ectoparasitic copepod caligus rogercresseyi that currently affects the chilean salmon industry. using high-throughput transcriptome sequencing, 56 cathepsin-like se ... | 2015 | 25923525 |
| sequence analysis of insecticide action and detoxification-related genes in the insect pest natural enemy pardosa pseudoannulata. | the pond wolf spider pardosa pseudoannulata, an important natural predatory enemy of rice planthoppers, is found widely distributed in paddy fields. however, data on the genes involved in insecticide action, detoxification, and response are very limited for p. pseudoannulata, which inhibits the development and appropriate use of selective insecticides to control insect pests on rice. we used transcriptome construction from adult spider cephalothoraxes to analyze and manually identify genes encon ... | 2015 | 25923714 |
| novel insights into the insect trancriptome response to a natural dna virus. | little is known about invertebrate responses to dna viruses. here, we infect a commercially important pest moth species plodia interpunctella with its naturally infecting dna virus. we sequenced, assembled and annotated the complete transcriptome of the moth, and a partial transcriptome of the virus. we then tested for differential gene expression between moths that were exposed to the virus and controls. | 2015 | 25924671 |
| small mosquitoes, large implications: crowding and starvation affects gene expression and nutrient accumulation in aedes aegypti. | environmental factors such as temperature, nutrient availability, and larval density determine the outcome of postembryonic development in mosquitoes. suboptimal temperatures, crowding, and starvation during the larval phase reduce adult mosquito size, nutrient stores and affect vectorial capacity. | 2015 | 25924822 |
| an improved odor bait for monitoring populations of aedes aegypti-vectors of dengue and chikungunya viruses in kenya. | effective surveillance and estimation of the biting fraction of aedes aegypti is critical for accurate determination of the extent of virus transmission during outbreaks and inter-epidemic periods of dengue and chikungunya fever. here, we describe the development and use of synthetic human odor baits for improved sampling of adult ae. aegypti, in two dengue and chikungunya fevers endemic areas in kenya; kilifi and busia counties. | 2015 | 25924877 |
| biocomputational characterization and evolutionary analysis of bubaline dicer1 enzyme. | dicer, an ribonuclease type iii type endonuclease, is the key enzyme involved in biogenesis of micrornas (mirnas) and small interfering rnas (sirnas), and thus plays a critical role in rna interference through post transcriptional regulation of gene expression. this enzyme has not been well studied in the indian water buffalo, an important species known for disease resistance and high milk production. in this study, the primary coding sequence (5,778 bp) of bubaline dicer (genbank: ab969677.1) w ... | 0 | 25925065 |
| the field evaluation of a push-pull system to control malaria vectors in northern belize, central america. | campaigns for the continued reduction and eventual elimination of malaria may benefit from new and innovative vector control tools. one novel approach being considered uses a push-pull strategy, whereby spatial repellents are used in combination with outdoor baited traps. the desired effect is the behavioural manipulation of mosquito populations to elicit movement of vectors away from people and into traps. | 2015 | 25925395 |
| the changing epidemiology of dengue in china, 1990-2014: a descriptive analysis of 25 years of nationwide surveillance data. | dengue has been a notifiable disease in china since 1 september 1989. cases have been reported each year during the past 25 years of dramatic socio-economic changes in china, and reached a historical high in 2014. this study describes the changing epidemiology of dengue in china during this period, to identify high-risk areas and seasons and to inform dengue prevention and control activities. | 2015 | 25925417 |
| the re-emergence of dengue in china. | the number of reports in the literature on dengue outbreaks in various parts of south china is increasing. this trend is likely contributed to by multiple factors, chief among which is the increase in trade and human movement in and out of china from the southeast asian region where dengue is firmly endemic. however, a holistic picture of dengue in china and how the public health authorities are responding to this global health challenge has been missing. in a research article published in bmc m ... | 2015 | 25925732 |
| microarray: a global analysis of biomineralization-related gene expression profiles during larval development in the pearl oyster, pinctada fucata. | the molluscan pinctada fucata is an important pearl-culturing organism to study biomineralization mechanisms. several biomineralization-related genes play important roles regulating shell formation, but most previous work has focused only on their functions in adult oysters. few studies have investigated biomineralization during larval development, when the shell is initially constructed and formed until the juvenile stage in dissoconch shells. here, we report, for the first time, a global gene ... | 2015 | 25927556 |
| larvicidal activity and possible mode of action of four flavonoids and two fatty acids identified in millettia pinnata seed toward three mosquito species. | aedes aegypti and aedes albopictus and culex pipiens pallens mosquitoes transmit dengue fever and west nile virus diseases, respectively. this study was conducted to determine the toxicity and mechanism of action of four flavonoids and two fatty acids from millettia pinnata (fabaceae) seed as well as six pure fatty acids and four fatty acid esters toward third instar larvae from insecticide-susceptible c. pipiens pallens and a. aegypti as well as wild a. albopictus. efficacy of 12 experimental l ... | 2015 | 25928224 |
| evolution of the rag1-rag2 locus: both proteins came from the same transposon. | the rag1 and rag2 proteins are essential subunits of the v(d)j recombinase that is required for the generation of the enormous variability of antibodies and t-cell receptors in jawed vertebrates. it was demonstrated previously that the 600-aa catalytic core of rag1 evolved from the transposase of the transib superfamily transposons. however, although homologs of rag1 and rag2 genes are adjacent in the purple sea urchin genome, a transposon encoding both proteins so far has not been reported. her ... | 2015 | 25928409 |
| effects of the p20 protein from bacillus thuringiensis israelensis on insecticidal crystal protein cry4ba. | the accessory protein p20 from bacillus thuringiensis israelensis has been defined as an important molecular chaperone for forming crystal cyt1aa, and enhancing cry11aa and cry4aa expression. to investigate its putative role in cry4ba delta-endotoxin production and toxicity, a p20 gene was cloned and introduced into b. thuringiensis recombinant strain expressing cry4ba type gene (cry4blb). the delta-endotoxin synthesis was enhanced by 262%. the generated inclusions were assayed against third ins ... | 2015 | 25931398 |
| costly inheritance and the persistence of insecticide resistance in aedes aegypti populations. | global emergence of arboviruses is a growing public health concern, since most of these diseases have no vaccine or prevention treatment available. in this scenario, vector control through the use of chemical insecticides is one of the most important prevention tools. nevertheless, their effectiveness has been increasingly compromised by the development of strong resistance observed in field populations, even in spite of fitness costs usually associated to resistance. using a stage-structured de ... | 2015 | 25933383 |
| wolbachia influences the production of octopamine and affects drosophila male aggression. | wolbachia bacteria are endosymbionts that infect approximately 40% of all insect species and are best known for their ability to manipulate host reproductive systems. though the effect wolbachia infection has on somatic tissues is less well understood, when present in cells of the adult drosophila melanogaster brain, wolbachia exerts an influence over behaviors related to olfaction. here, we show that a strain of wolbachia influences male aggression in flies, which is critically important in mat ... | 2015 | 25934616 |
| antennal transcriptome analysis of odorant reception genes in the red turpentine beetle (rtb), dendroctonus valens. | the red turpentine beetle (rtb), dendroctonus valens leconte (coleoptera: curculionidae, scolytinae), is a destructive invasive pest of conifers which has become the second most important forest pest nationwide in china. dendroctonus valens is known to use host odors and aggregation pheromones, as well as non-host volatiles, in host location and mass-attack modulation, and thus antennal olfaction is of the utmost importance for the beetles' survival and fitness. however, information on the genes ... | 2015 | 25938508 |
| a dengue virus type 4 model of disseminated lethal infection in ag129 mice. | dengue is a mosquito-borne disease of global public health significance that is caused by four serologically and genetically related viruses (denv-1 to denv-4). most human denv infections are asymptomatic, but clinical cases can range in severity from a relatively mild self-limiting illness to a severe life-threatening disease. infection with one serotype of denv results in life-long homotypic immunity but only short term heterotypic protection. there are no licensed vaccines or antivirals for d ... | 2015 | 25938762 |
| cd8+ t-cell responses in flavivirus-naive individuals following immunization with a live-attenuated tetravalent dengue vaccine candidate. | we are developing a live-attenuated tetravalent dengue vaccine (tdv) candidate based on an attenuated dengue 2 virus (tdv-2) and 3 chimeric viruses containing the premembrane and envelope genes of dengue viruses (denvs) -1, -3, and -4 expressed in the context of the attenuated tdv-2 genome (tdv-1, tdv-3, and tdv-4, respectively). in this study, we analyzed and characterized the cd8(+) t-cell response in flavivirus-naive human volunteers vaccinated with 2 doses of tdv 90 days apart via the subcut ... | 2015 | 25943203 |
| involvement of metabolic resistance and f1534c kdr mutation in the pyrethroid resistance mechanisms of aedes aegypti in india. | pesticide resistance poses a serious problem for worldwide mosquito control programs. resistance to insecticides can be caused by an increased metabolic detoxification of the insecticide and/or by target site insensitivity. in the present study, we estimated the tolerance of indian aedes aegypti populations using adult bioassays that revealed high resistance levels of the field populations to permethrin (rr-6, 5.8 and 5.1 folds) compared to our susceptible population. enzymatic assays revealed i ... | 2015 | 25944353 |
| fluid absorption in the isolated midgut of adult female yellow fever mosquitoes (aedes aegypti). | the transepithelial voltage (vte) and the volume of isolated posterior midguts of adult female yellow fever mosquitoes (aedes aegypti) were monitored. in all experiments, the initial vte after filling the midgut was lumen negative, but subsequently became lumen positive at a rate of approximately 1 mv min(-1). simultaneously, the midgut volume decreased, indicating spontaneous fluid absorption. when the midguts were filled and bathed with mosquito saline, the average rate of fluid absorption was ... | 2015 | 25944920 |
| structure of uridine diphosphate n-acetylglucosamine pyrophosphorylase from entamoeba histolytica. | uridine diphosphate n-acetylglucosamine pyrophosphorylase (uap) catalyzes the final step in the synthesis of udp-glcnac, which is involved in cell-wall biogenesis in plants and fungi and in protein glycosylation. small-molecule inhibitors have been developed against uap from trypanosoma brucei that target an allosteric pocket to provide selectivity over the human enzyme. a 1.8 å resolution crystal structure was determined of uap from entamoeba histolytica, an anaerobic parasitic protozoan that c ... | 2015 | 25945709 |
| mass trapping with mosquitraps does not reduce aedes aegypti abundance. | the objective of this study was to evaluate the effectiveness of aedes aegypti mass trapping using the sticky trap mosquitrap (mqt) by performing a cluster randomised controlled trial in manaus, state of amazonas, brazil. after an initial questionnaire and baseline monitoring of adult ae. aegypti abundance with bg-sentinel (bgs) traps in six clusters, three clusters were randomly assigned to the intervention arm where each participating household received three mqts for mass trapping during 17 m ... | 2015 | 25946154 |
| a public health risk assessment for yellow fever vaccination: a model exemplified by an outbreak in the state of são paulo, brazil. | we propose a method to analyse the 2009 outbreak in the region of botucatu in the state of são paulo (sp), brazil, when 28 yellow fever (yf) cases were confirmed, including 11 deaths. at the time of the outbreak, the secretary of health of the state of são paulo vaccinated one million people, causing the death of five individuals, an unprecedented number of yf vaccine-induced fatalities. we apply a mathematical model described previously to optimise the proportion of people who should be vaccina ... | 2015 | 25946247 |
| waiting for chikungunya fever in argentina: spatio-temporal risk maps. | chikungunya virus (chikv) transmission has been detected in america in 2013 and recently reached south up to bolivia, brazil and paraguay, bordering countries of argentina. the presence of the mosquito aedes aegypti in half of the country together with the regional context drove us to make a rapid assessment of transmission risk. temperature thresholds for vector breeding and for virus transmission, together with adult activity from the literature, were mapped on a monthly basis to estimate risk ... | 0 | 25946252 |
| double trouble: combined action of meiotic drive and wolbachia feminization in eurema butterflies. | arthropod sex ratios can be manipulated by a diverse range of selfish genetic elements, including maternally inherited wolbachia bacteria. feminization by wolbachia is rare but has been described for eurema mandarina butterflies. in this species, some phenotypic and functional females, thought to be zz genetic males, are infected with a feminizing wolbachia strain, wfem. meanwhile, heterogametic wz females are not infected with wfem. here, we establish a quantitative pcr assay allowing reliable ... | 0 | 25948567 |
| chemical composition and larvicidal activity of essential oils extracted from brazilian legal amazon plants against aedes aegypti l. (diptera: culicidae). | the mosquito aedes aegypti l. (diptera: culicidae) is the major vector of dengue and chikungunya fever. the lack of effective therapies and vaccines for these diseases highlights the need for alternative strategies to control the spread of virus. therefore, this study investigated the larvicidal potential of essential oils from common plant species obtained from the chapada das mesas national park, brazil, against third instar a. aegypti larvae. the chemical composition of these oils was determi ... | 2015 | 25949264 |
| in vitro antibacterial and in vivo cytotoxic activities of grewia paniculata. | grewia paniculata (family: malvaceae) has been used to treat inflammation, respiratory disorders and fever. it is additionally employed for other health conditions including colds, diarrhea and as an insecticide in bangladesh. the aim of the present study was to investigate the antibacterial and cytotoxic activities of different extracts of grewia paniculata. | 0 | 25949950 |
| a critical assessment of vector control for dengue prevention. | recently, the vaccines to vaccinate (v2v) initiative was reconfigured into the partnership for dengue control (pdc), a multi-sponsored and independent initiative. this redirection is consistent with the growing consensus among the dengue-prevention community that no single intervention will be sufficient to control dengue disease. the pdc's expectation is that when an effective dengue virus (denv) vaccine is commercially available, the public health community will continue to rely on vector cont ... | 2015 | 25951103 |
| effect of larval competition on extrinsic incubation period and vectorial capacity of aedes albopictus for dengue virus. | despite the growing awareness that larval competition can influence adult mosquito life history traits including susceptibility to pathogens, the net effect of larval competition on human risk of exposure to mosquito-borne pathogens remains poorly understood. we examined how intraspecific larval competition affects dengue-2 virus (denv-2) extrinsic incubation period and vectorial capacity of its natural vector aedes albopictus. adult ae. albopictus from low and high-larval density conditions wer ... | 2015 | 25951173 |
| high rate of subclinical chikungunya virus infection and association of neutralizing antibody with protection in a prospective cohort in the philippines. | chikungunya virus (chikv) is a globally re-emerging arbovirus for which previous studies have indicated the majority of infections result in symptomatic febrile illness. we sought to characterize the proportion of subclinical and symptomatic chikv infections in a prospective cohort study in a country with known chikv circulation. | 2015 | 25951202 |
| key enzymes and proteins of crop insects as candidate for rnai based gene silencing. | rna interference (rnai) is a mechanism of homology dependent gene silencing present in plants and animals. it operates through 21-24 nucleotides small rnas which are processed through a set of core enzymatic machinery that involves dicer and argonaute proteins. in recent past, the technology has been well appreciated toward the control of plant pathogens and insects through suppression of key genes/proteins of infecting organisms. the genes encoding key enzymes/proteins with the great potential ... | 2015 | 25954206 |
| novel detection of insecticide resistance related p450 genes and transcriptome analysis of the hemimetabolous pest erthesina fullo (thunberg) (hemiptera: heteroptera). | erthesina fullo (thunberg, 1783) is an economically important heteropteran species in china. since only three nucleotide sequences of this species (coi, 16s rrna, and 18s rrna) appear in the genbank database so far, no analysis of the molecular mechanisms underlying e. fullo's resistance to insecticide and environmental stress has been accomplished. we reported a de novo assembled and annotated transcriptome for adult e. fullo using the illumina sequence system. a total of 53,359,458 clean reads ... | 2015 | 25955554 |
| oxime derivatives with larvicidal activity against aedes aegypti l. | oximes containing secondary metabolites constitute an important group of bioactive compounds and have been described and frequently updated in the literature due to their pharmacological properties. thus, the aim of this study was to evaluate the larvicidal activity of a series of fourteen structurally related [1,4]-benzoquinone mono-oximes on third-instar aedes aegypti larvae and to investigate structure-activity relationships (sar) of these compounds. results of larvicidal assay revealed that ... | 2015 | 25956398 |
| the burden of dengue and chikungunya worldwide: implications for the southern united states and california. | dengue virus (denv) spreads to humans through the bite of an infected aedes aegypti or aedes albopictus mosquito and is a growing public health threat to both industrialized and developing nations worldwide. outbreaks of autochthonous dengue in the united states occurred extensively in the past but over the past 3 decades have again taken place in florida, hawaii, and texas as well as in american samoa, guam, northern mariana islands, puerto rico, and the us virgin islands. as the aedes vectors ... | 0 | 25960096 |
| morbidity rate prediction of dengue hemorrhagic fever (dhf) using the support vector machine and the aedes aegypti infection rate in similar climates and geographical areas. | in the past few decades, several researchers have proposed highly accurate prediction models that have typically relied on climate parameters. however, climate factors can be unreliable and can lower the effectiveness of prediction when they are applied in locations where climate factors do not differ significantly. the purpose of this study was to improve a dengue surveillance system in areas with similar climate by exploiting the infection rate in the aedes aegypti mosquito and using the suppo ... | 2015 | 25961289 |
| alphaviruses in gene therapy. | alphavirus vectors present an attractive approach for gene therapy applications due to the rapid and simple recombinant virus particle production and their broad range of mammalian host cell transduction. mainly three types of alphavirus vectors, namely naked rna, recombinant particles and dna/rna layered vectors, have been subjected to preclinical studies with the goal of achieving prophylactic or therapeutic efficacy, particularly in oncology. in this context, immunization with alphavirus vect ... | 2015 | 25961488 |
| the repellent deet potentiates carbamate effects via insect muscarinic receptor interactions: an alternative strategy to control insect vector-borne diseases. | insect vector-borne diseases remain one of the principal causes of human mortality. in addition to conventional measures of insect control, repellents continue to be the mainstay for personal protection. because of the increasing pyrethroid-resistant mosquito populations, alternative strategies to reconstitute pyrethroid repellency and knock-down effects have been proposed by mixing the repellent deet (n,n-diethyl-3-methylbenzamide) with non-pyrethroid insecticide to better control resistant ins ... | 2015 | 25961834 |
| costs of dengue in three french territories of the americas: an analysis of the hospital medical information system (pmsi) database. | dengue is a major emerging public health concern in tropical and subtropical countries. severe dengue can lead to hospitalisation and death. this study was performed to assess the economic burden of hospitalisations for dengue from 2007 to 2011 in three french territories of the americas where dengue is endemic (french guiana, martinique and guadeloupe). | 2015 | 25962729 |
| insect phylogenomics. | phylogenomics, the integration of phylogenetics with genome data, has emerged as a powerful approach to study the evolution and systematics of species. recently, several studies employing phylogenomic tools have provided better insights into insect evolution. next-generation sequencing methods are now increasingly used by entomologists to generate genomic and transcript sequences of various insect species and strains. these data provide opportunities for comparative genomics and large-scale mult ... | 2015 | 25963452 |
| diploid males support a two-step mechanism of endosymbiont-induced thelytoky in a parasitoid wasp. | haplodiploidy, where females develop from diploid, fertilized eggs and males from haploid, unfertilized eggs, is abundant in some insect lineages. some species in these lineages reproduce by thelytoky that is caused by infection with endosymbionts: infected females lay haploid eggs that undergo diploidization and develop into females, while males are very rare or absent. it is generally assumed that in thelytokous wasps, endosymbionts merely diploidize the unfertilized eggs, which would then tri ... | 2015 | 25963738 |
| is transfusion-transmitted dengue fever a potential public health threat? | dengue is an arboviruses due to single-stranded enveloped ribonucleic acid viruses, named dengue viruses (denv), that include four serotypes and are mainly transmitted via the bite of mosquitoes of the genus aedes (a. aegypti and a. albopictus). the distribution of the disease was historically limited to intertropical areas; however, during the last thirty years, the perimeter of the disease extended considerably and temperate areas are now at risk of outbreaks. the present global burden of deng ... | 0 | 25964876 |
| coffee and its waste repel gravid aedes albopictus females and inhibit the development of their embryos. | dengue is a prevalent arboviral disease and the development of insecticide resistance among its vectors impedes endeavors to control it. coffee is drunk by millions of people daily worldwide, which is associated with the discarding of large amounts of waste. coffee and its waste contain large amounts of chemicals many of which are highly toxic and none of which have a history of resistance in mosquitoes. once in solution, coffee is brownish in colour, resembling leaf infusion, which is highly at ... | 2015 | 25966847 |
| contrasting host-pathogen interactions and genome evolution in two generalist and specialist microsporidian pathogens of mosquitoes. | obligate intracellular pathogens depend on their host for growth yet must also evade detection by host defenses. here we investigate host adaptation in two microsporidia, the specialist edhazardia aedis and the generalist vavraia culicis, pathogens of disease vector mosquitoes. genomic analysis and deep rna-seq across infection time courses reveal fundamental differences between these pathogens. e. aedis retains enhanced cell surface modification and signalling capacity, upregulating protein tra ... | 2015 | 25968466 |
| imidacloprid impairs the post-embryonic development of the midgut in the yellow fever mosquito stegomyia aegypti (=aedes aegypti). | the mosquito stegomyia aegypti (=aedes aegypti) (diptera: culicidae) is a vector for the dengue and yellow fever viruses. as blood digestion occurs in the midgut, this organ constitutes the route of entry of many pathogens. the effects of the insecticide imidacloprid on the survival of st. aegypti were investigated and the sub-lethal effects of the insecticide on midgut development were determined. third instar larvae were exposed to different concentrations of imidacloprid (0.15, 1.5, 3.0, 6.0 ... | 2015 | 25968596 |
| dengue virus ns proteins inhibit rig-i/mavs signaling by blocking tbk1/irf3 phosphorylation: dengue virus serotype 1 ns4a is a unique interferon-regulating virulence determinant. | dengue virus (denv) replication is inhibited by the prior addition of type i interferon or by rig-i agonists that elicit rig-i/mavs/tbk1/irf3-dependent protective responses. denv infection of primary human endothelial cells (ecs) results in a rapid increase in viral titer, which suggests that denv inhibits replication-restrictive rig-i/interferon beta (ifn-β) induction pathways within ecs. our findings demonstrate that denv serotype 4 (denv4) nonstructural (ns) proteins ns2a and ns4b inhibited r ... | 2015 | 25968648 |
| a combination of doxycycline and ribavirin alleviated chikungunya infection. | lack of vaccine and effective antiviral drugs against chikungunya virus (chikv) outbreaks have led to significant impact on health care in the developing world. here, we evaluated the antiviral effects of tetracycline (tetra) derivatives and other common antiviral agents against chikv. our results showed that within the tetra derivatives group, doxycycline (doxy) exhibited the highest inhibitory effect against chikv replication in vero cells. on the other hand, in the antiviral group ribavirin ( ... | 2015 | 25970853 |
| do we need a vaccine against chikungunya? | during the last decade, the chikungunya (chikv) virus has expanded its range of activity, conquering new territories and becoming an important global health threat. in particular, the challenge represented by the recent emergence of chikv in the americas has strengthened the need of a safe and effective vaccine. although research on vaccines against chikv has been slow, a few vaccine candidates have been tested over the years. inactivated and attenuated vaccine candidates have shown promising re ... | 2015 | 25971340 |
| rotational symmetry of two pyrethroid receptor sites in the mosquito sodium channel. | voltage-gated sodium channels are the primary target of pyrethroid insecticides. although it is well known that specific mutations in insect sodium channels confer knockdown resistance (kdr) to pyrethroids, the atomic mechanisms of pyrethroid-sodium channel interactions are not clearly understood. previously, computer modeling and mutational analysis predicted two pyrethroid receptors, pyrethroid receptor site 1 (pyr1) (initial) and pyrethroid receptor site 2 (pyr2), located in the domain interf ... | 2015 | 25972447 |
| modeling the indirect effect of wolbachia on the infection dynamics of horizontally transmitted viruses. | intracellular bacteria of the genus wolbachia are widely distributed in arthropods. there is growing empirical evidence that wolbachia directly interacts with viruses and other parasites inside the arthropod host, sometimes resulting in low or no pathogen replication. previous theoretical studies showed that this direct effect of wolbachia can result in a reduced virus prevalence (within the population), suggesting that wolbachia could be used in the biological control of vector-borne diseases ( ... | 2015 | 25972858 |
| computational identification of novel micrornas and their targets in the malarial vector, anopheles stephensi. | micrornas are a ~22 nucleotide small non-coding rnas found in animals, plants and viruses. they regulate key cellular processes by enhancing, degrading or silencing protein coding targets. currently most of the data on mirna is available from drosophila . given their important post-transcriptional role in several organisms, there is a need to understand the mirna mediated processes in normal and abnormal conditions. here we report four novel micrornas ast - mir - 2502, ast - mir - 2559, ast - mi ... | 2015 | 25972985 |
| larvicidal activity against aedes aegypti of foeniculum vulgare essential oils from portugal and cape verde. | dengue is a potentially fatal mosquito-borne infection with 50 million cases per year and 2.5 billion people vulnerable to the disease. this major public health problem has recurrent epidemics in latin america and occurred recently in cape verde and madeira island. the lack of anti-viral treatment or vaccine makes the control of mosquito vectors a high option to prevent virus transmission. essential oil (eo) constituents can affect insect's behaviour, being potentially effective in pest control. ... | 2015 | 25973508 |
| schinus terebinthifolius leaf extract causes midgut damage, interfering with survival and development of aedes aegypti larvae. | in this study, a leaf extract from schinus terebinthifolius was evaluated for effects on survival, development, and midgut of a. aegypti fourth instar larvae (l4), as well as for toxic effect on artemia salina. leaf extract was obtained using 0.15 m nacl and evaluated for phytochemical composition and lectin activity. early l4 larvae were incubated with the extract (0.3-1.35%, w/v) for 8 days, in presence or absence of food. polymeric proanthocyanidins, hydrolysable tannins, heterosid and aglyco ... | 2015 | 25974067 |
| sero-prevalence of dengue infections in the kassala state in the eastern part of the sudan in 2011. | dengue fever is a vector-borne disease that is transmitted to humans by infected aedes aegypti mosquitoes. the eastern part of the sudan is one of the regions that is affected by dengue virus circulation. in this study, we estimated the prevalence of dengue infections in the kassala state in the eastern part of the sudan. the study objective was to estimate the sero-prevalence of dengue igg/igm antibodies in the kassala locality in 2011. this was a cross sectional community-based study that util ... | 2015 | 25975993 |
| emergence and potential for spread of chikungunya virus in brazil. | in december 2013, an outbreak of chikungunya virus (chikv) caused by the asian genotype was notified in the caribbean. the outbreak has since spread to 38 regions in the americas. by september 2014, the first autochthonous chikv infections were confirmed in oiapoque, north brazil, and in feira de santana, northeast brazil. | 2015 | 25976325 |
| mutual exclusion of asaia and wolbachia in the reproductive organs of mosquito vectors. | wolbachia is a group of intracellular maternally inherited bacteria infecting a high number of arthropod species. their presence in different mosquito species has been largely described, but aedes aegypti, the main vector of dengue virus, has never been found naturally infected by wolbachia. similarly, malaria vectors and other anophelines are normally negative to wolbachia, with the exception of an african population where these bacteria have recently been detected. asaia is an acetic acid bact ... | 2015 | 25981386 |
| mutant: a family of mutator-like transposable elements targeting ta microsatellites in medicago truncatula. | transposable elements (tes) are mobile dna segments, abundant and dynamic in plant genomes. because their mobility can be potentially deleterious to the host, a variety of mechanisms evolved limiting that negative impact, one of them being preference for a specific target insertion site. here, we describe a family of mutator-like dna transposons in medicago truncatula targeting ta microsatellites. we identified 218 copies of mutants and an element carrying a complete orf encoding a mudra-like tr ... | 2015 | 25981486 |
| experience- and age-mediated oviposition behaviour in the yellow fever mosquito stegomyia aegypti (=aedes aegypti). | in repeated behaviours such as those of feeding and reproduction, past experiences can inform future behaviour. by altering their behaviour in response to environmental stimuli, insects in highly variable landscapes can tailor their behaviour to their particular environment. in particular, female mosquitoes may benefit from plasticity in their choice of egg-laying site as these sites are often temporally variable and clustered. the opportunity to adapt egg-laying behaviour to past experience als ... | 2015 | 25982411 |