Publications

biological control of mosquitoes in scrap tires in brownsville, texas, usa and matamoros, tamaulipas, mexico.	dengue periodically circulates in southern texas and neighboring tamaulipas, mexico; thus, a closer examination of human and vector ecology at the northern limits of north american transmission may improve prevention activities. scrap tires produce large mosquito populations and increase the risk of dengue transmission. some households choose not to pay tire disposal fees, and many tires are illegally dumped in residential areas. biological control may provide low-cost and environmentally friend ...	2014	25102598
mosquito communities in nova iguaçu natural park, rio de janeiro, brazil.	abstract. in order to gather information on the culicid fauna of nova iguaçu municipal park, rio de janeiro, brazil, adult and immature stages were collected with the centers for disease control and prevention miniature light traps, and dippers and suction tubes, respectively. in all, 828 adult and 990 immature specimens were collected belonging to 12 genera. among the species collected were aedes aegypti, ae. albopictus, ae. fluviatilis, ae. scapularis, haemagogus leucocelaenus, and psorophora ...	2014	25102590
standardization of a bottle assay--an indigenous method for laboratory and field monitoring of insecticide resistance and comparison with who adult susceptibility test.	the who adult susceptibility test is in use for insecticide resistance monitoring. presently, materials are being imported from the universiti sains malaysia, malaysia and sometimes it is cost prohibitive. as an alternative, we present here a method of bottle bioassay using indigenous material. different aspects related to the assay were studied and validated in the field. bottle assay was standardized in the laboratory by using locally sourced material and laboratory-maintained insecticide-susc ...	2014	25098343
repellency of essential oils extracted from thai native plants against aedes aegypti (linn.) and culex quinquefasciatus (say).	repellent activity of essential oils derived from 10 thai native plants, belonging to three families were evaluated against female aedes aegypti and culex quinquefasciatus and to compare them with a commercial chemical repellents (deet; n,n-diethyl-3-methylbenzamide 20% w/w; sketolene shield). each test repellent was applied at 1, 5, and 10% concentrations for testing by arm in cage method. the results showed significant differences in repellency among the repellents by mosquito species. the pro ...	2014	25088471
[dengue fever in mainland france].	dengue fever is the most widespread distributed vector borne viral disease. it is transmitted through the bites of aedes aegypti and aedes albopictus mosquitoes. with the expansion of aedes albopictus and increasing travel exchange, it is no longer limited to the tropical zone and transmission has been documented in temperate areas. in mainland france, where aedes albopictus has been present and disseminating since 2004, 2 episodes of autochthonous transmission occurred in 2010 and in 2013. cont ...	2014	25080833
hot temperatures can force delayed mosquito outbreaks via sequential changes in aedes aegypti demographic parameters in autocorrelated environments.	aedes aegypti l. (diptera: culicidae) is a common pantropical urban mosquito, vector of dengue, yellow fever and chikungunya viruses. studies have shown ae. aegypti abundance to be associated with environmental fluctuations, revealing patterns such as the occurrence of delayed mosquito outbreaks, i.e., sudden extraordinary increases in mosquito abundance following transient extreme high temperatures. here, we use a two-stage (larvae and adults) matrix model to propose a mechanism for environment ...	2014	23537497
implications of saline concentrations for the performance and competitive interactions of the mosquitoes aedes aegypti (stegomyia aegypti) and aedes albopictus (stegomyia albopictus).	aedes albopictus (stegomyia albopictus) (diptera: culicidae) has probably supplanted aedes aegypti (stegomyia aegypti) throughout most of its historical range in the u.s.a., although ae. aegypti still exists in large coastal cities in southern florida. we measured salt concentrations in field containers along an axis perpendicular to the coast and examined intraspecific outcomes in these species under different salt concentrations in a factorial study using varying intra- and interspecific densi ...	2014	23607885
residual and pre-treatment application of starycide insect growth regulator (triflumuron) to control aedes aegypti in containers.	dengue is not endemic in australia, although the vector mosquito aedes aegypti is. in order to prevent dengue establishment, australia maintains tight control of vector mosquitoes. tight control programs often necessitate regular repeat application of treatments, and this is resource intensive. we sought to investigate the efficacy and longevity of triflurumon starycide® insect growth regulator under field conditions in tropical australia against aedes. we compared two doses (0.48 and 0.96 ppm), ...	2014	23653423
chemometric studies on potential larvicidal compounds against aedes aegypti.	the mosquito aedes aegypti (diptera, culicidae) is the vector of yellow and dengue fever. in this study, chemometric tools, such as, principal component analysis (pca), consensus pca (cpca), and partial least squares regression (pls), were applied to a set of fifty five active compounds against ae. aegypti larvae, which includes terpenes, cyclic alcohols, phenolic compounds, and their synthetic derivatives. the calculations were performed using the volsurf+ program. cpca analysis suggests that t ...	2014	23676010
the diel copulation periodicity of the mosquito, aedes aegypti (l.) (diptera: culicidae) at indoor and outdoor sites in trinidad, west indies.	the diel copulation periodicity of domestic aedes aegypti in the field in trinidad, west indies was monitored weekly at indoor and outdoor sites over a 16 month period. at indoor sites two significant (p<0.02) peaks in copulation occurred between 06.00 and 08.00 (25% of copulation events) and between 16.00 and 18.00 (24%): at outdoor sites the copulation periodicity was similar to that observed indoors with an early morning peak in copulation at 06.00-08.00 (30%) and a late evening peak at 16.00 ...	2014	23850504
modelling interventions during a dengue outbreak.	we present a stochastic dynamical model for the transmission of dengue that considers the co-evolution of the spatial dynamics of the vectors (aedes aegypti) and hosts (human population), allowing the simulation of control strategies adapted to the actual evolution of an epidemic outbreak. we observed that imposing restrictions on the movement of infected humans is not a highly effective strategy. in contrast, isolating infected individuals with high levels of compliance by the human population ...	2014	23800514
will people change their vector-control practices in the presence of an imperfect dengue vaccine?	human behaviours, which are influenced by social, cultural, economic and political factors, can increase or decrease the risk of dengue infection, depending on the relationship with the insect vector. because no vaccine is currently available, the spread of dengue can only be curtailed by controlling vector populations (aedes aegypti and others) and by protecting individuals. this study tested the hypothesis that dengue-affected populations are likely to relax their vector-control habits if a po ...	2014	23735007
seasonal and nonseasonal dynamics of aedes aegypti in rio de janeiro, brazil: fitting mathematical models to trap data.	mathematical models suggest that seasonal transmission and temporary cross-immunity between serotypes can determine the characteristic multi-year dynamics of dengue fever. seasonal transmission is attributed to the effect of climate on mosquito abundance and within host virus dynamics. in this study, we validate a set of temperature and density dependent entomological models that are built-in components of most dengue models by fitting them to time series of ovitrap data from three distinct neig ...	2014	23933186
[aedes aegypti l. and aedes albopictus skuse mosquitoes are a new biological threat to the south of russia].		2014	23088153
[determination in vivo of the role of esterase and glutathione transferase enzymes in pyrethroid resistance of aedes aegypti (diptera: culicidae)].	an in vivo study of two synergists, that is, triphenil phosphate -specific esterase inhibitor- and ethacrynic acid -specific gluthation transferase inhibitor- was performed to determine if these enzymes were responsible for pyrethroid resistance of aedes aegypti. to this end, two insecticide resistant aedes aegypti strains were used, one strain selected with temephos by six selection generations (san-f6) and the other strain with delmamethrin by 12 selection generations (san-f12), being both str ...	2014	23427458
[insecticide resistance mechanisms of aedes aegypti (diptera: culicidae) from two peruvian provinces].	insecticide resistance of aedes aegypti larvae and adults from two peruvian provinces, that is, trujillo and tumbes provinces, was conducted. high infestation indexes and extensive use of insecticides based on the vector surveillance and control strategy of the ministry of public health prevailed in these places. larval bioassays revealed susceptibility to organophosphorate insecticide called malathion in trujillo strain, it being moderate to fention and fenitrotion and high to chlorpyriphos and ...	2014	23427457
invasion of aedes albopictus (diptera: culicidae) into central africa: what consequences for emerging diseases?	aedes albopictus, a mosquito native to asia, has invaded all five continents during the past three decades. it was reported in central africa in the 2000s, first in cameroon, and, since then, has colonised almost all countries of the region. the species, originally considered a secondary vector of dengue viruses, has been showed to play a major role in transmission of chikungunya virus in numerous countries, including in the central african region. we review the current spread of ae. albopictus ...	2015	25885461
the first imported case infected with chikungunya virus in korea.	chikungunya is caused by an arbovirus transmitted by aedes mosquito vector. with the increase of habitat of mosquito by global warming and frequent international travel and interchange, chikungunya reemerged and showed global distribution recently. until now there has not been reported any case infected with chikungunya virus in korea. a 23-year-old man has been the republic of the philippines for 1 week, and visited our emergency center due to fever and back pain. chikungunya viral infection wa ...	2015	25844264
proteomics profiling of chikungunya-infected aedes albopictus c6/36 cells reveal important mosquito cell factors in virus replication.	chikungunya virus (chikv) is the only causative agent of chikv fever with persistent arthralgia, and in some cases may lead to neurological complications which can be highly fatal, therefore it poses severe health issues in many parts of the world. chikv transmission can be mediated via the aedes albopictus mosquito; however, very little is currently known about the involvement of mosquito cellular factors during chikv-infection within the mosquito cells. unravelling the neglected aspects of mos ...	2015	25738640
a multipurpose, high-throughput single-nucleotide polymorphism chip for the dengue and yellow fever mosquito, aedes aegypti.	the dengue and yellow fever mosquito, aedes aegypti, contributes significantly to global disease burden. genetic study of aedes aegypti is essential to understanding its evolutionary history, competence as a disease vector, and the effects and efficacy of vector control methods. the prevalence of repeats and transposable elements in the aedes aegypti genome complicates marker development and makes genome-wide genetic study challenging. to overcome these challenges, we developed a high-throughput ...	2015	25721127
a case of diabetic ketoacidosis following chikungunya virus infection.	chikungunya is a mosquito-borne viral disease that has recently become endemic in the caribbean, including the island of puerto rico. we present the case of a 50-year-old puerto rican man who traveled to st. louis for business and was diagnosed with acute chikungunya virus infection with atypical features causing diabetic ketoacidosis. this case highlights the need to keep tropical infectious diseases on the differential diagnosis in appropriate individuals and the ways in which tropical infecti ...	2015	26033023
influence of breeding site availability on the oviposition behaviour of aedes aegypti.	despite the importance of the mosquito aedes aegypti in the transmission of arboviruses, such as yellow fever, chikungunya fever and dengue fever, some aspects of their behaviour remain unknown. in the present study, the oviposition behaviour of ae. aegypti females that were exposed to different densities of breeding sites (2, 4, 8 and 16) was evaluated in laboratory and semi-field conditions. the number of breeding sites that were used was proportional to the number available, but tended toward ...	2015	26154742
complete genome sequence of chikungunya virus isolated from an aedes aegypti mosquito during an outbreak in yemen, 2011.	chikungunya virus is recognized as a serious public health problem. the complete genome was sequenced for a chikungunya virus isolated from the mosquito aedes aegypti during a 2011 outbreak in al hodayda, yemen, which resulted in significant human fatalities. phylogenetic analysis demonstrated that this yemeni isolate is most closely related to indian ocean strains of the east/central/south african genotype.	2015	26184944
an outbreak of chikungunya in rural bangladesh, 2011.	the first identified chikungunya outbreak occurred in bangladesh in 2008. in late october 2011, a local health official from dohar sub-district, dhaka district, reported an outbreak of undiagnosed fever and joint pain. we investigated the outbreak to confirm the etiology, describe the clinical presentation, and identify associated vectors.	2015	26161995
differential analysis of the secretome of wrl68 cells infected with the chikungunya virus.	the chikungunya virus (chikv) is an arthropod borne virus. in the last 50 years, it has been the cause of numerous outbreaks in tropical and temperate regions, worldwide. there is limited understanding regarding the underlying molecular mechanisms involved in chikv replication and how the virus interacts with its host. in the present study, comparative proteomics was used to identify secreted host proteins that changed in abundance in response to early chikv infection. two-dimensional gel electr ...	2015	26083627
caribbean and la réunion chikungunya virus isolates differ in their capacity to induce proinflammatory th1 and nk cell responses and acute joint pathology.	chikungunya virus (chikv) is a mosquito-borne arthralgic alphavirus that has garnered international attention as an important emerging pathogen since 2005. more recently, it invaded the caribbean islands and the western hemisphere. intriguingly, the current chikv outbreak in the caribbean is caused by the asian chikv genotype, which differs from the la réunion lr2006 opy1 isolate belonging to the indian ocean lineage. here, we adopted a systematic and comparative approach against lr2006 opy1 to ...	2015	25995257
chikungunya virus transmission potential by local aedes mosquitoes in the americas and europe.	chikungunya virus (chikv), mainly transmitted in urban areas by the mosquitoes aedes aegypti and aedes albopictus, constitutes a major public health problem. in late 2013, chikv emerged on saint-martin island in the caribbean and spread throughout the region reaching more than 40 countries. thus far, ae. aegypti mosquitoes have been implicated as the sole vector in the outbreaks, leading to the hypothesis that chikv spread could be limited only to regions where this mosquito species is dominant.	2015	25993633
a combination of doxycycline and ribavirin alleviated chikungunya infection.	lack of vaccine and effective antiviral drugs against chikungunya virus (chikv) outbreaks have led to significant impact on health care in the developing world. here, we evaluated the antiviral effects of tetracycline (tetra) derivatives and other common antiviral agents against chikv. our results showed that within the tetra derivatives group, doxycycline (doxy) exhibited the highest inhibitory effect against chikv replication in vero cells. on the other hand, in the antiviral group ribavirin ( ...	2015	25970853
high rate of subclinical chikungunya virus infection and association of neutralizing antibody with protection in a prospective cohort in the philippines.	chikungunya virus (chikv) is a globally re-emerging arbovirus for which previous studies have indicated the majority of infections result in symptomatic febrile illness. we sought to characterize the proportion of subclinical and symptomatic chikv infections in a prospective cohort study in a country with known chikv circulation.	2015	25951202
seroepidemiological survey of chikungunya in and around the regions of bijapur (vijayapura - north karnataka).	chikungunya is a debilitating, non-fatal, mosquito borne viral fever caused by chikungunya virus (chiva). the disease is transmitted to humans by the bite of aedes aegypti and aedes albopictus mosquitoes. severe outbreaks of chikungunya have been reported in several countries of africa and asia. chikungunya fever is characterized by fever with sudden onset, arthralgia, rash, headache and myalgia. however, arthralgia is painful and long-lasting, affecting primarily the peripheral joints.	2015	26155474
small mosquitoes, large implications: crowding and starvation affects gene expression and nutrient accumulation in aedes aegypti.	environmental factors such as temperature, nutrient availability, and larval density determine the outcome of postembryonic development in mosquitoes. suboptimal temperatures, crowding, and starvation during the larval phase reduce adult mosquito size, nutrient stores and affect vectorial capacity.	2015	25924822
socio-economic and climate factors associated with dengue fever spatial heterogeneity: a worked example in new caledonia.	understanding the factors underlying the spatio-temporal distribution of infectious diseases provides useful information regarding their prevention and control. dengue fever spatio-temporal patterns result from complex interactions between the virus, the host, and the vector. these interactions can be influenced by environmental conditions. our objectives were to analyse dengue fever spatial distribution over new caledonia during epidemic years, to identify some of the main underlying factors, a ...	2015	26624008
potential of selected senegalese aedes spp. mosquitoes (diptera: culicidae) to transmit zika virus.	zika virus (zikv; genus flavivirus, family flaviviridae) is an emerging virus of medical importance maintained in a zoonotic cycle between arboreal aedes spp. mosquitoes and nonhuman primates in african and asian forests. serological evidence and virus isolations have demonstrated widespread distribution of the virus in senegal. several mosquito species have been found naturally infected by zikv but little is known about their vector competence.	2015	26527535
short-term suppression of aedes aegypti using genetic control does not facilitate aedes albopictus.	under permit from the national biosafety commission for the use of genetically modified organisms, releases of a genetically engineered self-limiting strain of aedes aegypti (ox513a) were used to suppress urban pest ae. aegypti in west panama. experimental goals were to assess the effects on a coexisting population of ae. albopictus and examine operational parameters with relevance to environmental impact.	2015	26374668
detection of chikungunya virus in nepal.	chikungunya virus (chikv) is an emerging alphaviral disease and a public health problem in south asia including nepal in recent years. in this study, sera were collected from patients presenting with fever, headache, muscular pain, fatigue, and joint pain of both upper and lower extremities. a total of 169 serum samples were tested for chikv and dengue virus (denv) by using immunoglobulin m (igm) and immunoglobulin g (igg) antibody using enzyme-linked immunosorbent assay (elisa) method during au ...	2015	26195462
psychiatric morbidity in patients with chikungunya fever: first report from india.	chikungunya fever is an acute illness caused by an arbovirus and has various complications like neurological, psychological, dermatological and even multi organ failure. psychiatric co-morbidity is not very well studied till now. this is the first report from india.	2015	26557595
french invasive asian tiger mosquito populations harbor reduced bacterial microbiota and genetic diversity compared to vietnamese autochthonous relatives.	the asian tiger mosquito aedes albopictus is one of the most significant pathogen vectors of the twenty-first century. originating from asia, it has invaded a wide range of eco-climatic regions worldwide. the insect-associated microbiota is now recognized to play a significant role in host biology. while genetic diversity bottlenecks are known to result from biological invasions, the resulting shifts in host-associated microbiota diversity has not been thoroughly investigated. to address this su ...	2015	26441903
the chikungunya epidemic: a look at five cases.	chikungunya is an infection caused by the chikungunya virus and transmitted by the bite of infected mosquito. the most common symptoms of chikungunya virus infection are fever, joint pain or rash. chikungunya virus outbreaks had been identified in countries in africa, asia, europe, and the indian and pacific oceans. in late 2013, the first local transmission of chikungunya virus in the americas was identified in caribbean countries and territories. chikungunya virus disease became a nationally n ...	2015	26793467
fitness cost in field and laboratory aedes aegypti populations associated with resistance to the insecticide temephos.	the continued use of chemical insecticides in the context of the national program of dengue control in brazil has generated a high selective pressure on the natural populations of aedes aegypti, leading to their resistance to these compounds in the field. fitness costs have been described as adaptive consequences of resistance. this study evaluated the biological and reproductive performance of a. aegypti strains and a field population resistant to temephos, the main larvicide used for controlli ...	2015	26715037
correlation of clinicohaematological parameters in paediatric dengue: a retrospective study.	dengue is one of the arthropod-borne (arbo) viral diseases transmitted by female mosquito aedes aegypti. dengue fever has a wide spectrum of clinical presentation ranging from flu-like illness to severe complicated stage of dengue hemorrhagic fever leading to mortality. this was a retrospective study conducted in a tertiary care hospital in coastal karnataka, south india, to know the correlation between the clinical presentation and haematological parameters in the paediatric cases presented wit ...	2015	26819620
peridomestic infection as a determining factor of dengue transmission.	the study of endemic dengue transmission is essential for proposing alternatives to impact its burden. the traditional paradigm establishes that transmission starts around cases, but there are few studies that determine the risk.	2015	26671573
human social behavior and demography drive patterns of fine-scale dengue transmission in endemic areas of colombia.	dengue is known to transmit between humans and a. aegypti mosquitoes living in neighboring houses. although transmission is thought to be highly heterogeneous in both space and time, little is known about the patterns and drivers of transmission in groups of houses in endemic settings. we carried out surveys of pcr positivity in children residing in 2-block patches of highly endemic cities of colombia. we found high levels of heterogeneity in pcr positivity, varying from less than 30% in 8 of th ...	2015	26656072
assessing the presence of wuchereria bancrofti in vector and human populations from urban communities in conakry, guinea.	the global programme to eliminate lymphatic filariasis was launched in 2000 with the goal of interrupting transmission of lymphatic filariasis (lf) through multiple rounds of mass drug administration (mda). in guinea, there is evidence of ongoing lf transmission, but little is known about the most densely populated parts of the country, including the capital conakry. in order to guide the lf control and elimination efforts, serological and entomological surveys were carried out to determine whet ...	2015	26410739
annona muricata (annonaceae): a review of its traditional uses, isolated acetogenins and biological activities.	annona muricata is a member of the annonaceae family and is a fruit tree with a long history of traditional use. a. muricata, also known as soursop, graviola and guanabana, is an evergreen plant that is mostly distributed in tropical and subtropical regions of the world. the fruits of a. muricata are extensively used to prepare syrups, candies, beverages, ice creams and shakes. a wide array of ethnomedicinal activities is contributed to different parts of a. muricata, and indigenous communities ...	2015	26184167
interaction of a dengue virus ns1-derived peptide with the inhibitory receptor kir3dl1 on natural killer cells.	killer immunoglobulin-like receptors (kirs) interact with human leucocyte antigen (hla) class i ligands and play a key role in the regulation and activation of nk cells. the functional importance of kir-hla interactions has been demonstrated for a number of chronic viral infections, but to date only a few studies have been performed in the context of acute self-limited viral infections. during our investigation of cd8(+) t cell responses to a conserved hla-b57-restricted epitope derived from den ...	2015	26439909
production of infectious dengue virus in aedes aegypti is dependent on the ubiquitin proteasome pathway.	dengue virus (denv) relies on host factors to complete its life cycle in its mosquito host for subsequent transmission to humans. denv first establishes infection in the midgut of aedes aegypti and spreads to various mosquito organs for lifelong infection. curiously, studies have shown that infectious denv titers peak and decrease thereafter in the midgut despite relatively stable viral genome levels. however, the mechanisms that regulate this decoupling of infectious virion production from vira ...	2015	26566123
a re-examination of the history of etiologic confusion between dengue and chikungunya.	contrary to the perception of many researchers that the recent invasion of chikungunya (chik) in the western hemisphere marked the first episode in history, a recent publication reminded them that chik had prevailed in the west indies and southern regions of the united states from 1827-1828 under the guise of "dengue" (den), and that many old outbreaks of so-called "dengue" actually represented the chik cases erroneously identified as "dengue." in hindsight, this confusion was unavoidable, given ...	2015	26562299
dengue virus infection alters post-transcriptional modification of micrornas in the mosquito vector aedes aegypti.	recent discoveries regarding the importance of isomirs have increased our understanding of the regulatory complexities of the mirnaome. observed changes in the mirna profiles in mosquitoes infected with flaviviruses have implicated small rnas in the interactions between viruses and their vectors. here we analysed the isomir profiles of both uninfected and infected aedes aegypti mosquitoes with the major human pathogen dengue virus (denv). we found that several specific isomirs were significantly ...	2015	26514826
the effects of socioeconomic and environmental factors on the incidence of dengue fever in the pearl river delta, china, 2013.	an outbreak of dengue fever (df) occurred in guangdong province, china in 2013 with the highest number of cases observed within the preceding ten years. df cases were clustered in the pearl river delta economic zone (prd) in guangdong province, which accounted for 99.6% of all cases in guangdong province in 2013. the main vector in prd was aedes albopictus. we investigated the socioeconomic and environmental factors at the township level and explored how the independent variables jointly affect ...	2015	26506616
wolbachia and the insect immune system: what reactive oxygen species can tell us about the mechanisms of wolbachia-host interactions.	wolbachia are intracellular bacteria that infect a vast range of arthropod species, making them one of the most prevalent endosymbionts in the world. wolbachia's stunning evolutionary success is mostly due to their reproductive parasitism but also to mutualistic effects such as increased host fecundity or protection against pathogens. however, the mechanisms underlying wolbachia phenotypes, both parasitic and mutualistic, are only poorly understood. moreover, it is unclear how the insect immune ...	2015	26579107
copulation activity, sperm production and conidia transfer in aedes aegypti males contaminated by metarhizium anisopliae: a biological control prospect.	dengue is the most prevalent arboviral disease transmitted by aedes aegypti worldwide, whose chemical control is difficult, expensive, and of inconsistent efficacy. releases of metarhizium anisopliae--exposed ae. aegypti males to disseminate conidia among female mosquitoes by mating represents a promising biological control approach against this important vector. a better understanding of fungus virulence and impact on reproductive parameters of ae. aegypti, is need before testing auto-dissemina ...	2015	26473490
economic burden of dengue virus infection at the household level among residents of puerto maldonado, peru.	dengue virus (denv) was reintroduced to peru in the 1990s and has been reported in puerto maldonado (population ~65,000) in the peruvian southern amazon basin since 2000. this region also has the highest human migration rate in the country, mainly from areas not endemic for denv. the objective of this study was to assess the proportion of household income that is diverted to costs incurred because of dengue illness and to compare these expenses between recent migrants (rms) and long-term residen ...	2015	26217040
region-wide synchrony and traveling waves of dengue across eight countries in southeast asia.	dengue is a mosquito-transmitted virus infection that causes epidemics of febrile illness and hemorrhagic fever across the tropics and subtropics worldwide. annual epidemics are commonly observed, but there is substantial spatiotemporal heterogeneity in intensity. a better understanding of this heterogeneity in dengue transmission could lead to improved epidemic prediction and disease control. time series decomposition methods enable the isolation and study of temporal epidemic dynamics with a s ...	2015	26438851
widespread signatures of local mrna folding structure selection in four dengue virus serotypes.	it is known that mrna folding can affect and regulate various gene expression steps both in living organisms and in viruses. previous studies have recognized functional rna structures in the genome of the dengue virus. however, these studies usually focused either on the viral untranslated regions or on very specific and limited regions at the beginning of the coding sequences, in a limited number of strains, and without considering evolutionary selection.	2015	26449467
a survey of the 2014 dengue fever epidemic in guangzhou, china.	in 2014, a serious dengue outbreak in guangzhou occurred, consisting of 37 354 laboratory confirmed cases of infection. in this study, the clinical picture of dengue fever due to dengue virus (denv) type 1 in guangzhou was described. clinical and laboratory data collected by studying 726 sera of suspected clinical cases from hospitals and 328 sera of healthy persons from two residence communities were analyzed during the outbreak, and 484 patients were diagnosed with an acute dengue infection. f ...	2015	26954995
gene expression changes in the salivary glands of anopheles coluzzii elicited by plasmodium berghei infection.	malaria is a devastating infectious disease caused by plasmodium parasites transmitted through the bites of infected anopheles mosquitoes. salivary glands are the only mosquito tissue invaded by plasmodium sporozoites, being a key stage for the effective parasite transmission, making the study of anopheles sialome highly relevant.	2015	26395987
a dengue outbreak on a floating village at cat ba island in vietnam.	a dengue outbreak in an ecotourism destination spot in vietnam, from september to november 2013, impacted a floating village of fishermen on the coastal island of cat ba. the outbreak raises questions about how tourism may impact disease spread in rural areas.	2015	26395076
dengue: etiology of acute febrile illness in abidjan, côte d'ivoire, in 2011-2012.	the burden of dengue in africa is not well understood. a prospective study was conducted in abidjan, côte d'ivoire from december 2011 to december 2012 to estimate the proportion of dengue and malaria cases among febrile patients during a period when dengue was not known to be circulating in the region, and to describe the clinical and virological characteristics of laboratory-diagnosed dengue cases.	2015	26385938
inferring protective cd8+ t-cell epitopes for ns5 protein of four serotypes of dengue virus chinese isolates based on hla-a, -b and -c allelic distribution: implications for epitope-based universal vaccine design.	dengue is one of the most globally serious vector-borne infectious diseases in tropical and subtropical areas for which there are currently no effective vaccines. the most highly conserved flavivirus protein, ns5, is an indispensable target of cd8+ t-cells, making it an ideal vaccine design target. using the immune epitope database (iedb), cd8+ t-cell epitopes of the dengue virus (denv) ns5 protein were predicted by genotypic frequency of the hla-a,-b, and-c alleles in chinese population. antige ...	2015	26381649
use of centrifugal filter devices to concentrate dengue virus in mosquito per os infection experiments.	dengue virus (denv) is an arbovirus transmitted to humans by the bite of infected aedes mosquitoes. experimental per os infection of mosquitoes with denv is usually a preliminary step in virus/vector studies but it requires being able to prepare artificial blood-meals with high virus titers. we report here the convenient use of centrifugal filter devices to quickly concentrate denv particles in cell-culture supernatants. the median viral titer in concentrated-supernatants was 8.50 log10 tcid50/m ...	2015	26372347
identification of essential containers for aedes larval breeding to control dengue in dhaka, bangladesh.	dengue fever (df), one of the most important emerging arboviral diseases, is transmitted through the bite of container breeding mosquitoes aedes aegypti and aedes albopictus. a household entomological survey was conducted in dhaka from august through october 2000 to inspect water-holding containers in indoor, outdoor, and rooftop locations for aedes larvae. the objective of this study was to determine mosquito productivity of each container type and to identify some risk factors of households in ...	2015	26865829
impact of human mobility on the emergence of dengue epidemics in pakistan.	the recent emergence of dengue viruses into new susceptible human populations throughout asia and the middle east, driven in part by human travel on both local and global scales, represents a significant global health risk, particularly in areas with changing climatic suitability for the mosquito vector. in pakistan, dengue has been endemic for decades in the southern port city of karachi, but large epidemics in the northeast have emerged only since 2011. pakistan is therefore representative of ...	2015	26351662
epidemiological scenario of dengue in brazil.	dengue is the most important reemerging mosquito-borne viral disease worldwide. it is caused by any of four dengue virus types or serotypes (denv-1 to denv-4) and is transmitted by mosquitoes from the genus aedes. ecological changes have favored the geographic expansion of the vector and, since the dengue pandemic in the asian and pacific regions, the infection became widely distributed worldwide, reaching brazil in 1845. the incidence of dengue in brazil has been frequently high, and the number ...	2015	26413514
source and purity of dengue-viral preparations impact requirement for enhancing antibody to induce elevated il-1β secretion: a primary human monocyte model.	dengue virus is a major global health threat and can lead to life-threatening hemorrhagic complications due to immune activation and cytokine production. cross-reactive antibodies to an earlier dengue virus infection are a recognized risk factor for severe disease. these antibodies bind heterologous dengue serotypes and enhance infection into fc-receptor-bearing cells, a process known as antibody-dependent enhancement of infection. one crucial cytokine seen elevated in severe dengue patients is ...	2015	26301593
regulation of gene expression patterns in mosquito reproduction.	in multicellular organisms, development, growth and reproduction require coordinated expression of numerous functional and regulatory genes. insects, in addition to being the most speciose animal group with enormous biological and economical significance, represent outstanding model organisms for studying regulation of synchronized gene expression due to their rapid development and reproduction. disease-transmitting female mosquitoes have adapted uniquely for ingestion and utilization of the hug ...	2015	26274815
expression of dengue virus ns3 protein in drosophila alters its susceptibility to infection.	we developed a drosophila model in which the dengue virus ns3 protein is expressed in a tissue specific and inducible manner. dengue virus ns3 is a multifunctional protein playing a major role during viral replication. both protease and helicase domains of ns3 are interacting with human and insect host proteins including innate immune components of the host machinery. we characterized the ns3 transgenic flies showing that ns3 expression did not affect fly development. to further study the links ...	2015	26267447
cross-sectional community-based study of the socio-demographic factors associated with the prevalence of dengue in the eastern part of sudan in 2011.	dengue is caused by an arthropod-borne flavivirus. infection can be either primary or secondary based on serology, with each stage of the disease characterized by specific serological conversion and antibody formation. further study is needed to fully identify the factors associated with and predisposing to dengue infection. the objective of this study was to identify socio-demographic factors associated with the prevalence of dengue serotypes in kassala state in the eastern part of sudan in 201 ...	2015	26084275
activation of tlr2 and tlr6 by dengue ns1 protein and its implications in the immunopathogenesis of dengue virus infection.	dengue virus (dv) infection is the most prevalent mosquito-borne viral disease and its manifestation has been shown to be contributed in part by the host immune responses. in this study, pathogen recognition receptors, toll-like receptor (tlr) 2 and tlr6 were found to be up-regulated in dv-infected human pbmc using immunofluorescence staining, flow cytometry and western blot analyses. using elisa, il-6 and tnf-α, cytokines downstream of tlr2 and tlr6 signaling pathways were also found to be up-r ...	2015	26226614
characterization of n-glycan structures on the surface of mature dengue 2 virus derived from insect cells.	denv envelope glycoprotein (e) is responsible for interacting with host cell receptors and is the main target for the development of a dengue vaccine based on an induction of neutralizing antibodies. it is well known that denv e glycoprotein has two potential n-linked glycosylation sites at asn67 and asn153. the n-glycans of e glycoprotein have been shown to influence the proper folding of the protein, its cellular localization, its interactions with receptors and its immunogenicity. however, th ...	2015	26208004
interaction of mean temperature and daily fluctuation influences dengue incidence in dhaka, bangladesh.	local weather influences the transmission of the dengue virus. most studies analyzing the relationship between dengue and climate are based on relatively coarse aggregate measures such as mean temperature. here, we include both mean temperature and daily fluctuations in temperature in modelling dengue transmission in dhaka, the capital of bangladesh. we used a negative binomial generalized linear model, adjusted for rainfall, anomalies in sea surface temperature (an index for el niño-southern os ...	2015	26161895
evidence based community mobilization for dengue prevention in nicaragua and mexico (camino verde, the green way): cluster randomized controlled trial.	to test whether community mobilization adds effectiveness to conventional dengue control.	2015	26156323
the global compendium of aedes aegypti and ae. albopictus occurrence.	aedes aegypti and ae. albopictus are the main vectors transmitting dengue and chikungunya viruses. despite being pathogens of global public health importance, knowledge of their vectors' global distribution remains patchy and sparse. a global geographic database of known occurrences of ae. aegypti and ae. albopictus between 1960 and 2014 was compiled. herein we present the database, which comprises occurrence data linked to point or polygon locations, derived from peer-reviewed literature and un ...	2015	26175912
targeted release and fractionation reveal glucuronylated and sulphated n- and o-glycans in larvae of dipteran insects.	mosquitoes are important vectors of parasitic and viral diseases with anopheles gambiae transmitting malaria and aedes aegypti spreading yellow and dengue fevers. using two different approaches (solid-phase extraction and reversed-phase or hydrophilic interaction hplc fractionation followed by maldi-tof ms or permethylation followed by nsi-ms), we examined the n-glycans of both a. gambiae and a. aegypti larvae and demonstrate the presence of a range of paucimannosidic glycans as well as bi- and ...	2015	26047717
reproduction-immunity trade-offs in insects.	immune defense and reproduction are physiologically and energetically demanding processes and have been observed to trade off in a diversity of female insects. increased reproductive effort results in reduced immunity, and reciprocally, infection and activation of the immune system reduce reproductive output. this trade-off can manifest at the physiological level (within an individual) and at the evolutionary level (genetic distinction among individuals in a population). the resource allocation ...	2015	26667271
comparative transcriptomic analysis of immune responses of the migratory locust, locusta migratoria, to challenge by the fungal insect pathogen, metarhizium acridum.	the migratory locust, locusta migratoria manilensis, is an immensely destructive agricultural pest that forms a devastating and voracious gregarious phase. the fungal insect pathogen, metarhizium acridum, is a specialized locust pathogen that has been used as a potent mycoinsecticide for locust control. little, however, is known about locust immune tissue, i.e. fat body and hemocyte, responses to challenge by this fungus.	2015	26503342
transcriptomic analysis of musca domestica to reveal key genes of the prophenoloxidase-activating system.	the propo system regulates melanization in arthropods. however, the genes that are involved in the propo system in housefly musca domestica remain unclear. thus, this study analyzed the combined transcriptome obtained from m. domestica larvae, pupae, and adults that were either normal or bacteria-challenged by an escherichia coli and staphylococcus aureus mixture. a total of 54,821,138 clean reads (4.93 gb) were yielded by illumina sequencing, which were de novo assembled into 89,842 unigenes. o ...	2015	26156588
overexpression of tyrosine hydroxylase and dopa decarboxylase associated with pupal melanization in spodoptera exigua.	melanism has been found in a wide range of species, but the molecular mechanisms involved remain largely elusive. in this study, we studied the molecular mechanisms of the pupal melanism in spodoptera exigua. the full length cdna sequences of tyrosine hydroxylase (th) and dopa decarboxylase (ddc), two key enzymes in the biosynthesis pathway of melanin, were cloned, and their temporal expression patterns in the integument were compared during the larval-pupal metamorphosis process of the s. exigu ...	2015	26084938
key enzymes and proteins of crop insects as candidate for rnai based gene silencing.	rna interference (rnai) is a mechanism of homology dependent gene silencing present in plants and animals. it operates through 21-24 nucleotides small rnas which are processed through a set of core enzymatic machinery that involves dicer and argonaute proteins. in recent past, the technology has been well appreciated toward the control of plant pathogens and insects through suppression of key genes/proteins of infecting organisms. the genes encoding key enzymes/proteins with the great potential ...	2015	25954206
exome and transcriptome sequencing of aedes aegypti identifies a locus that confers resistance to brugia malayi and alters the immune response.	many mosquito species are naturally polymorphic for their abilities to transmit parasites, a feature which is of great interest for controlling vector-borne disease. aedes aegypti, the primary vector of dengue and yellow fever and a laboratory model for studying lymphatic filariasis, is genetically variable for its capacity to harbor the filarial nematode brugia malayi. the genome of ae. aegypti is large and repetitive, making genome resequencing difficult and expensive. we designed exome captur ...	2015	25815506
fipronil and permethrin combination: a novel ectoparasiticide for dogs.		2015	25622552
an overview of malaria transmission from the perspective of amazon anopheles vectors.	in the americas, areas with a high risk of malaria transmission are mainly located in the amazon forest, which extends across nine countries. one keystone step to understanding the plasmodium life cycle in anopheles species from the amazon region is to obtain experimentally infected mosquito vectors. several attempts to colonise anopheles species have been conducted, but with only short-lived success or no success at all. in this review, we review the literature on malaria transmission from the ...	2015	25742262
evaluation of a protocol for remote identification of mosquito vector species reveals bg-sentinel trap as an efficient tool for anopheles gambiae outdoor collection in burkina faso.	feasibility and costs of monitoring efforts aimed to monitor mosquito species are strictly dependent on the presence of skilled entomologists directly in the field. however, in several contexts this is not possible or easy to organize, thus limiting the possibility to obtain crucial information on presence/abundance of potential disease vectors and of new invasive species. digital imaging approaches could be extremely useful in the frame of medical entomology to overcome this limit. this work de ...	2015	25888896
rotational symmetry of two pyrethroid receptor sites in the mosquito sodium channel.	voltage-gated sodium channels are the primary target of pyrethroid insecticides. although it is well known that specific mutations in insect sodium channels confer knockdown resistance (kdr) to pyrethroids, the atomic mechanisms of pyrethroid-sodium channel interactions are not clearly understood. previously, computer modeling and mutational analysis predicted two pyrethroid receptors, pyrethroid receptor site 1 (pyr1) (initial) and pyrethroid receptor site 2 (pyr2), located in the domain interf ...	2015	25972447
unraveling dual feeding associated molecular complexity of salivary glands in the mosquito anopheles culicifacies.	mosquito salivary glands are well known to facilitate meal acquisition, however the fundamental question on how adult female salivary gland manages molecular responses during sugar versus blood meal uptake remains unanswered. to investigate these responses, we analyzed a total of 58.5 million raw reads generated from two independent rnaseq libraries of the salivary glands collected from 3-4 day-old sugar and blood fed anopheles culicifacies mosquitoes. comprehensive functional annotation analysi ...	2015	26163527
small-scale evaluation of the efficacy and residual activity of alpha-cypermethrin wg (250 g ai/kg) for indoor spraying in comparison with alpha-cypermethrin wp (50 g ai/kg) in india.	indoor residual spraying (irs) with different formulations of insecticides is being used for the control of mosquito vectors in many countries. in the present study, residual efficacy and duration of effectiveness of irs with alpha-cypermethrin wg-sb (250 g ai/m2) formulation was compared with wp formulation (50 g ai/kg) in a small scale (phase ii) field trial.	2015	26022085
diversification of the genus anopheles and a neotropical clade from the late cretaceous.	the anopheles genus is a member of the culicidae family and consists of approximately 460 recognized species. the genus is composed of 7 subgenera with diverse geographical distributions. despite its huge medical importance, a consensus has not been reached on the phylogenetic relationships among anopheles subgenera. we assembled a comprehensive dataset comprising the coi, coii and 5.8s rrna genes and used maximum likelihood and bayesian inference to estimate the phylogeny and divergence times o ...	2015	26244561
modified mosquito landing boxes dispensing transfluthrin provide effective protection against anopheles arabiensis mosquitoes under simulated outdoor conditions in a semi-field system.	efforts to control malaria vectors have primarily focused on scaling-up of long-lasting insecticidal nets (llins) and indoor residual spraying. although highly efficient against indoor-biting and indoor-resting vectors, these interventions have lower impact on outdoor-biting mosquitoes. innovative vector control tools are required to prevent outdoor human-mosquito contacts. in this work, the potential of spatial repellents, delivered in an active system that requires minimal user compliance, to ...	2015	26104719
long non-coding rna discovery across the genus anopheles reveals conserved secondary structures within and beyond the gambiae complex.	long non-coding rnas (lncrnas) have been defined as mrna-like transcripts longer than 200 nucleotides that lack significant protein-coding potential, and many of them constitute scaffolds for ribonucleoprotein complexes with critical roles in epigenetic regulation. various lncrnas have been implicated in the modulation of chromatin structure, transcriptional and post-transcriptional gene regulation, and regulation of genomic stability in mammals, caenorhabditis elegans, and drosophila melanogast ...	2015	25903279
long-lasting permethrin-impregnated clothing protects against mosquito bites in outdoor workers.	outdoor exposure to mosquitoes is a risk factor for many diseases, including malaria and dengue. we have previously shown that long-lasting permethrin-impregnated clothing protects against tick and chigger bites in a double-blind randomized controlled trial in north carolina outdoor workers. here, we evaluated whether this clothing is protective against mosquito bites by measuring changes in antibody titers to mosquito salivary gland extracts. on average, there was a 10-fold increase in titer du ...	2015	26195460
how diverse detrital environments influence nutrient stoichiometry between males and females of the co-occurring container mosquitoes aedes albopictus, ae. aegypti, and culex quinquefasciatus.	allocation patterns of carbon and nitrogen in animals are influenced by food quality and quantity, as well as by inherent metabolic and physiological constraints within organisms. whole body stoichiometry also may vary between the sexes who differ in development rates and reproductive allocation patterns. in aquatic containers, such as tree holes and tires, detrital inputs, which vary in amounts of carbon and nitrogen, form the basis of the mosquito-dominated food web. differences in development ...	2015	26244643
fungal biological control agents for integrated management of culicoides spp. (diptera: ceratopogonidae) of livestock.	entomopathogenic fungi metarhizium anisopliae and beauveria bassiana had wide host range against insects and hence these are being exploited as fungal bio-pesticide on a large scale. both fungi are proved pesticides against many crop pests and farmers are well acquainted with their use on the field. thus, research was aimed to explore the potency of these fungal spores against larval and adult culicoides midges, a pest of livestock.	2015	27047065
microsatellite loci cross-species transferability in aedes fluviatilis (diptera:culicidae): a cost-effective approach for population genetics studies.	aedes fluviatilis is a neotropical mosquito species thought to be a potential vector of yellow fever viruses and can be infected with plasmodium gallinaceum in laboratory. a better understanding of its genetic structure is very important to understand its epidemiologic potential and how it is responding to urbanization. the objective of this study was to survey the transferability of microsatellites loci developed for other aedes to ae. fluviatilis.	2015	26667177
making mosquito taxonomy useful: a stable classification of tribe aedini that balances utility with current knowledge of evolutionary relationships.	the tribe aedini (family culicidae) contains approximately one-quarter of the known species of mosquitoes, including vectors of deadly or debilitating disease agents. this tribe contains the genus aedes, which is one of the three most familiar genera of mosquitoes. during the past decade, aedini has been the focus of a series of extensive morphology-based phylogenetic studies published by reinert, harbach, and kitching (rh&k). those authors created 74 new, elevated or resurrected genera from wha ...	2015	26226613
approaches to passive mosquito surveillance in the eu.	the recent emergence in europe of invasive mosquitoes and mosquito-borne disease associated with both invasive and native mosquito species has prompted intensified mosquito vector research in most european countries. central to the efforts are mosquito monitoring and surveillance activities in order to assess the current species occurrence, distribution and, when possible, abundance, in order to permit the early detection of invasive species and the spread of competent vectors. as active mosquit ...	2015	25567671
characterization and expression profiling of glutathione s-transferases in the diamondback moth, plutella xylostella (l.).	glutathione s-transferases (gsts) are multifunctional detoxification enzymes that play important roles in insects. the completion of several insect genome projects has enabled the identification and characterization of gst genes over recent years. this study presents a genome-wide investigation of the diamondback moth (dbm), plutella xylostella, a species in which the gsts are of special importance because this pest is highly resistant to many insecticides.	2015	25887517
remarkable repellency of ligusticum sinense (umbelliferae), a herbal alternative against laboratory populations of anopheles minimus and aedes aegypti (diptera: culicidae).	for personal protection against mosquito bites, user-friendly natural repellents, particularly from plant origin, are considered as a potential alternative to applications currently based on synthetics such as deet, the standard chemical repellent. this study was carried out in thailand to evaluate the repellency of ligusticum sinense hexane extract (lhe) against laboratory anopheles minimus and aedes aegypti, the primary vectors of malaria and dengue fever, respectively.	2015	26249666
generation of recombinant oropouche viruses lacking the nonstructural protein nsm or nss.	oropouche virus (orov) is a midge-borne human pathogen with a geographic distribution in south america. orov was first isolated in 1955, and since then, it has been known to cause recurring outbreaks of a dengue-like illness in the amazonian regions of brazil. orov, however, remains one of the most poorly understood emerging viral zoonoses. here we describe the successful recovery of infectious orov entirely from cdna copies of its genome and generation of orov mutant viruses lacking either the ...	2015	26699638
detection of insemination status in live aedes aegypti females.	using the technique described in this report, the presence or absence of sperm in spermathecae of female aedes aegypti is detectable without dissection. spermathecae of a lightly anesthetized female can be visualized by phase contrast microscopy through the distended abdomen, after the intersegmental membranes are stretched by ventral placement of a glass cover slip. most females recovered after the procedure were capable of subsequent reproductive activities. albeit tedious, this technique pres ...	2015	25721054
biocontrol evaluation of extracts and a major component, clusianone, from clusia fluminensis planch. & triana against aedes aegypti.	studies evaluated the effects of hexanic extracts from the fruits and flowers of clusia fluminensis and the main component of the flower extract, a purified benzophenone (clusianone), against aedes aegypti. the treatment of larvae with the crude fruit or flower extracts from c. fluminensis did not affect the survival ofae. aegypti (50 mg/l), however, the flower extracts significantly delayed development of ae. aegypti. in contrast, the clusianone (50 mg/l) isolate from the flower extract, repres ...	2015	26200711
functional characterization of aquaporins and aquaglyceroporins of the yellow fever mosquito, aedes aegypti.	after taking vertebrate blood, female mosquitoes quickly shed excess water and ions while retaining and concentrating the mostly proteinaceous nutrients. aquaporins (aqps) are an evolutionary conserved family of membrane transporter proteins that regulate the flow of water and in some cases glycerol and other small molecules across cellular membranes. in a previous study, we found six putative aqp genes in the genome of the yellow fever mosquito, ae. aegypti, and demonstrated the involvement of ...	2015	25589229