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fabrication of highly effective mosquito nanolarvicides using an asian plant of ethno-pharmacological interest, priyangu (aglaia elaeagnoidea): toxicity on non-target mosquito natural enemies.mosquitoes threaten the lives of humans, livestock, pets and wildlife around the globe, due to their ability to vector devastating diseases. aglaia elaeagnoidea, commonly known as priyangu, is widely employed in asian traditional medicine and pest control. medicinal activities include anti-inflammatory, analgesic, anticancer, and anesthetic actions. flavaglines, six cyclopenta[b]benzofurans, a cyclopenta[bc]benzopyran, a benzo[b]oxepine, and an aromatic butyrolactone showed antifungal properties ...201728390026
toxicity of herbal extracts used in ethno-veterinary medicine and green-encapsulated zno nanoparticles against aedes aegypti and microbial pathogens.dengue and chikungunya are arboviral diseases mainly vectored by the mosquito aedes aegypti. presently, there is no treatment for these viral diseases and their prevention is still based on vector control measures. nanopesticides fabricated using herbal extracts as reducing and capping agents currently represent an excellent platform for pest control. in this scenario, the present study assessed the acute toxicity of seven plants employed in ethno-veterinary medicine of southern india, as well a ...201728389893
new records of aedes aegypti in southern oklahoma, 2016.aedes aegypti is an important subtropical vector species and is predicted to have a limited year-round distribution in the southern united states. collection of the species has not been officially verified in oklahoma since 1940. adult mosquitoes were collected in 42 sites across 7 different cities in oklahoma using 3 different mosquito traps between may and september 2016. between july and september 2016, 88 ae. aegypti adults were collected at 18 different sites in 4 different cities across so ...201728388329
insecticidal and repellent activity of several plant-derived essential oils against aedes aegypti.we examined the pupicidal, adulticidal, repellent, and oviposition-deterrent activities of essential oils (eos) from lippia alba, l. origanoides, eucalyptus citriodora, cymbopogon citratus, cymbopogon flexuosus, citrus sinensis , cananga odorata , swinglea glutinosa, and tagetes lucida plants against aedes aegypti under laboratory conditions. pupicidal and adulticidal activities were assessed at exploratory concentrations of 250, 310, and 390 parts per million (ppm); and 30, 300, and 1,000 ppm, ...201728388322
entomological investigations during early stages of a chikungunya outbreak in the united states virgin islands, 2014.during the 2014 chikungunya (chik) outbreak in the caribbean, we performed entomological surveys on 3 united states virgin islands (usvi): st. croix, st. thomas, and st. john. we aimed to evaluate the potential for chikungunya virus (chikv) transmission in the usvi. the surveys took place between june 19, 2014, and june 29, 2014, during the dry season in usvi. a total of 1,929 adult mosquitoes belonging to 4 species- culex quinquefasciatus (68.4%), aedes aegypti (29.7%), ae. mediovittatus (1.3%) ...201728388320
optimal control problems of mosquito-borne disease subject to changes in feeding behavior of aedes mosquitoes.dengue viruses (denv) are transmitted to humans by the bite of aedes mosquitoes. it is known that dengue virus infection in aedes aegypti female mosquitoes makes a change in the feeding behavior of the infected mosquitoes. in this study, using the forces of infection, we incorporated the effect of changes in the feeding behavior of mosquitoes into the standard vector-borne sir-si model. it has been proved that both a single-strain model and a two-strain model exhibit forward bifurcations. moreov ...201728385591
in the hunt for genomic markers of metabolic resistance to pyrethroids in the mosquito aedes aegypti: an integrated next-generation sequencing approach.the capacity of aedes mosquitoes to resist chemical insecticides threatens the control of major arbovirus diseases worldwide. until alternative control tools are widely deployed, monitoring insecticide resistance levels and identifying resistance mechanisms in field mosquito populations is crucial for implementing appropriate management strategies. metabolic resistance to pyrethroids is common in aedes aegypti but the monitoring of the dynamics of resistant alleles is impeded by the lack of robu ...201728379969
catalase protects aedes aegypti from oxidative stress and increases midgut infection prevalence of dengue but not zika.digestion of blood in the midgut of aedes aegypti results in the release of pro-oxidant molecules that can be toxic to the mosquito. we hypothesized that after a blood meal, the antioxidant capacity of the midgut is increased to protect cells against oxidative stress. concomitantly, pathogens present in the blood ingested by mosquitoes, such as the arboviruses dengue and zika, also have to overcome the same oxidative challenge, and the antioxidant program induced by the insect is likely to influ ...201728379952
effects of socio-demographic characteristics and household water management on aedes aegypti production in suburban and rural villages in laos and thailand.dengue fever is a mosquito-borne disease accounting for 50-100 million annual cases globally. laos and thailand are countries in south-east asia where the disease is endemic in both urban and rural areas. household water storage containers, which are favourable breeding sites for dengue mosquitoes, are common in these areas, due to intermittent or limited access to water supply. this study assessed the effect of household water management and socio-demographic risk factors on aedes aegypti infes ...201728376893
emergence of zika virus epidemic and the national response in jamaica.jamaica, along with the americas, experienced major epidemics of arboviral diseases transmitted by the aedes aegypti mosquito in recent years. these include dengue fever in 2012, chikungunya fever in 2014 and zika virus infection (zikv) in 2016. we present the emergence of the zikv epidemic in jamaica and outline the national response.201628375542
new insights into the in vitro development and virulence of culicinomyces spp. as fungal pathogens of aedes aegypti.culicinomyces spp. (hypocreales: cordycipitaceae) are facultative fungal pathogens affecting the larval stages from a range of mosquito species and are especially notable in their ability to infect hosts through the digestive tract after conidial ingestion. while culicinomyces spp. were studied mainly in the 1980s, little is yet known about inter- and intraspecific variability of the in vitro development of these fungi at different temperatures, and nothing is known about the impact of serial ho ...201728372997
erratum: efficacy of some wearable devices compared with spray-on insect repellents for the yellow fever mosquito, aedes aegypti (l.) (diptera: culicidae). 201728365772
inhibition of zika virus by wolbachia in aedes aegypti.through association with cases of microcephaly in 2015, zika virus (zikv) has transitioned from a relatively unknown mosquito-transmitted pathogen to a global health emergency, emphasizing the need to improve existing mosquito control programs to prevent future disease outbreaks. the response to zika must involve a paradigm shift from traditional to novel methods of mosquito control, and according to the world health organization should incorporate the release of mosquitoes infected with the bac ...201628357366
does zika virus cause microcephaly - applying the bradford hill viewpoints.zika virus has been documented since 1952, but been associated with mild, self-limiting disease. zika virus is classified as an arbovirus from a family of flaviviridae and primarily spread by aedes aegypti mosquitos. however, in a large outbreak in brazil in 2015, zika virus has been associated with microcephaly.201728357156
evolutionary origin and function of nox4-art, an arthropod specific nadph oxidase.nadph oxidases (nox) are ros producing enzymes that perform essential roles in cell physiology, including cell signaling and antimicrobial defense. this gene family is present in most eukaryotes, suggesting a common ancestor. to date, only a limited number of phylogenetic studies of metazoan noxes have been performed, with few arthropod genes. in arthropods, only nox5 and duox genes have been found and a gene called noxm was found in mosquitoes but its origin and function has not been examined. ...201728356077
larvicidal activity of ottonia anisum metabolites against aedes aegypti: a potential natural alternative source for mosquito vector control in brazil.aedes aegypti mosquito is the principal vector of the viruses responsible for urban yellow fever, dengue, dengue haemorrhagic fever, as well as zika and chikungunya in brazil. the present study was aimed to investigate the insecticidal potential of the extract and fractions of ottonia anisum, along with special metabolites isolated from it, as natural alternatives against larvae (l3) of ae. aegypti, vector of potentially deadly tropical infections in brazil.201728352047
host-virus interaction of zika virus in modulating disease pathogenesis.the zika virus (zikv) is a newly emerging pathogen that has resulted in a worldwide epidemic. it primarily spreads either through infected aedes aegypti or aedes albopictus mosquitos leading to severe neurological disorders such as microcephaly and guillain-barré syndrome in susceptible individuals. the mode of zikv entry into specific cell types such as: epidermal keratinocytes, fibroblasts, immature dendritic cells (idcs), and stem-cell-derived human neural progenitors has been determined thro ...201728349242
combined target site (kdr) mutations play a primary role in highly pyrethroid resistant phenotypes of aedes aegypti from saudi arabia.pyrethroid resistance is a threat to effective vector control of aedes aegypti, the vector of dengue, zika and other arboviruses, but there are many major knowledge gaps on the mechanisms of resistance. in jeddah and makkah, the principal dengue-endemic areas of saudi arabia, pyrethroids are used widely for ae. aegypti control but information about resistance remains sparse, and the underlying genetic basis is unknown. findings from an ongoing study in this internationally significant area are r ...201728347352
first report of the phe1534cys kdr mutation in natural populations of aedes albopictus from brazil.knockdown resistance (kdr), caused by alterations in the voltage-gated sodium channel (nav), is one of the mechanisms responsible for pyrethroid (py) resistance. in the asian tiger mosquito, aedes albopictus, at least four different mutations were described in the iiis6 nav segment in populations from asia, north america and europe. in contrast, in aedes aegypti at least 12 non-synonymous mutations have been reported at nine different codons, mostly in the iis6 and iiis6 nav segments. the phe153 ...201728347326
potential of aedes aegypti and aedes albopictus populations in the central african republic to transmit enzootic chikungunya virus strains.major chikungunya outbreaks have affected several central african countries during the past decade. the chikungunya virus (chikv) was isolated from humans and sylvan mosquitoes in the central african republic (car) during the 1970 and 1980s but has not been found recently, despite the presence of aedes albopictus since 2010. the risk of a massive chikungunya epidemic is therefore potentially high, as the human populations are immunologically naïve and because of the presence of the mosquito vect ...201728347325
the toxicity of angiotensin converting enzyme inhibitors to larvae of the disease vectors aedes aegypti and anopheles gambiae.the control of mosquitoes is threatened by the appearance of insecticide resistance and therefore new control chemicals are urgently required. here we show that inhibitors of mosquito peptidyl dipeptidase, a peptidase related to mammalian angiotensin-converting enzyme (ace), are insecticidal to larvae of the mosquitoes, aedes aegypti and anopheles gambiae. ace inhibitors (captopril, fosinopril and fosinoprilat) and two peptides (trypsin-modulating oostatic factor/tmof and a bradykinin-potentiati ...201728345667
novel synthesis of gold nanoparticles using artemisia vulgaris l. leaf extract and their efficacy of larvicidal activity against dengue fever vector aedes aegypti l.the aedes aegypti l. mosquito transmits dengue and yellow fever, which cause millions of death every year. dengue is a mosquito-borne viral disease that has rapidly spread worldwide particularly in countries with tropical and subtropical climates areas. the present study denotes a simple and eco-friendly biosynthesis of gold nanoparticles using artemisia vulgaris l. leaf extract as reducing agent. the synthesized gold nanoparticles were characterized by uv-visible spectroscopy, x-ray diffraction ...201728341392
preparing the united states for zika virus: pre-emptive vector control and personal protection.discovered in 1947 in a monkey in the zika forest of uganda, zika virus was dismissed as a cause of a mild illness that was confined to africa and southeast asia and transmitted by aedes mosquitoes. in 2007, zika virus appeared outside of its endemic borders in an outbreak on the south pacific island of yap. in 2013, zika virus was associated with a major neurological complication, guillain-barré syndrome, in a larger outbreak in the french polynesian islands. from the south pacific, zika invade ...201628340908
rapid surveillance for vector presence (rsvp): development of a novel system for detecting aedes aegypti and aedes albopictus.the globally important zika, dengue and chikungunya viruses are primarily transmitted by the invasive mosquitoes, aedes aegypti and aedes albopictus. in australia, there is an increasing risk that these species may invade highly urbanized regions and trigger outbreaks. we describe the development of a rapid surveillance for vector presence (rsvp) system to expedite presence- absence surveys for both species.201728339458
spatial quantification of the world population potentially exposed to zika virus.zika virus is an emerging flaviviridae virus, which has spread rapidly in the last few years. it has raised concern because it has been associated with fetus microcephaly when pregnant women are infected. the main vector is the mosquito aedes aegypti , distributed in tropical areas.201728338754
de novo assembly of the aedes aegypti genome using hi-c yields chromosome-length scaffolds.the zika outbreak, spread by the aedes aegypti mosquito, highlights the need to create high-quality assemblies of large genomes in a rapid and cost-effective way. here we combine hi-c data with existing draft assemblies to generate chromosome-length scaffolds. we validate this method by assembling a human genome, de novo, from short reads alone (67× coverage). we then combine our method with draft sequences to create genome assemblies of the mosquito disease vectors aeaegypti and culex quinquefa ...201728336562
functional and nonfunctional forms of cquior91, an odorant selectivity subunit of culex quinquefasciatus.in culex quinquefasciatus, cquior91 is the ortholog of 2 larvae-specific odorant receptors (ors) from anopheles gambiae (agam\or40, previously shown to respond to several odorant ligands including the broad-spectrum repellent n,n-diethyl-3-methylbenzamide, deet) and aedes aegypti (aaeg\or40). when we cloned full-length cquior91 from a culex quinquefasciatus larval head rna sample, we found 2 alleles of this or, differing at 9 residues. functional analysis using the xenopus oocyte expression syst ...201728334229
the relationship between entomological indicators of aedes aegypti abundance and dengue virus infection.routine entomological monitoring data are used to quantify the abundance of ae. aegypti. the public health utility of these indicators is based on the assumption that greater mosquito abundance increases the risk of human denv transmission, and therefore reducing exposure to the vector decreases incidence of infection. entomological survey data from two longitudinal cohort studies in iquitos, peru, linked with 8,153 paired serological samples taken approximately six months apart were analyzed. i ...201728333938
screening of wolbachia endosymbiont infection in aedes aegypti mosquitoes using attenuated total reflection mid-infrared spectroscopy.dengue fever is the most common mosquito transmitted viral infection afflicting humans, estimated to generate around 390 million infections each year in over 100 countries. the introduction of the endosymbiotic bacterium wolbachia into aedes aegypti mosquitoes has the potential to greatly reduce the public health burden of the disease. this approach requires extensive polymerase chain reaction (pcr) testing of the wolbachia-infection status of mosquitoes in areas where wolbachia-a. aegypti are r ...201728332822
boswellia ovalifoliolata (burseraceae) essential oil as an eco-friendly larvicide? toxicity against six mosquito vectors of public health importance, non-target mosquito fishes, backswimmers, and water bugs.the use of synthetic pesticides to control vector populations is detrimental to human health and the environment and may lead to the development of resistant strains. plants can be alternative sources of safer compounds effective on mosquito vectors. in this study, the mosquito larvicidal activity of boswellia ovalifoliolata leaf essential oil (eo) was evaluated against anopheles stephensi, anopheles subpictus, aedes aegypti, aedes albopictus, culex quinquefasciatus, and culex tritaeniorhynchus. ...201728332087
ascension island: a survey to assess the presence of zika virus vectors.following concerns regarding the spread of zika virus, joint services health unit (cyprus) were tasked to carry out a mosquito survey on the ascension island, south atlantic. this was to determine if vectors of the virus such as aedes aegypti and aedes albopictus were introduced and established on the island.201728331047
spatial-temporal analysis of dengue deaths: identifying social vulnerabilities.currently, dengue fever, chikungunya fever, and zika virus represent serious public health issues in brazil, despite efforts to control the vector, the aedes aegypti mosquito.201728327810
a new paradigm for aedes spp. surveillance using gravid ovipositing sticky trap and ns1 antigen test kit.dengue remains a serious public health problem in southeast asia and has increased 37-fold in malaysia compared to decades ago. new strategies are urgently needed for early detection and control of dengue epidemics.201728327173
potential high-risk areas for zika virus transmission in the contiguous united states.to understand where transmission of zika virus has the highest likelihood to occur in the contiguous united states with regard to its transmission both sexually and via aedes aegypti mosquito bites.201728323468
biologic evidence required for zika disease enhancement by dengue antibodies.the sudden appearance of overt human zika virus infections that cross the placenta to damage fetal tissues, target sexual organs, and are followed in some instances by guillain-barré syndrome raises questions regarding whether these outcomes are caused by genetic mutations or if prior infection by other flaviviruses affects disease outcome. because dengue and zika viruses co-circulate in the urban aedes aegypti mosquito-human cycle, a logical question, as suggested by in vitro data, is whether d ...201728322690
retention of duplicated long-wavelength opsins in mosquito lineages by positive selection and differential expression.opsins are light sensitive receptors associated with visual processes. insects typically possess opsins that are stimulated by ultraviolet, short and long wavelength (lw) radiation. six putative lw-sensitive opsins predicted in the yellow fever mosquito, aedes aegypti and malaria mosquito, anopheles gambiae, and eight in the southern house mosquito, culex quinquefasciatus, suggest gene expansion in the family culicidae (mosquitoes) relative to other insects. here we report the first detailed mol ...201728320313
occurrence of concurrent infections with multiple serotypes of dengue viruses during 2013-2015 in northern kerala, india.dengue is a global human public health threat, causing severe morbidity and mortality. the occurrence of sequential infection by more than one serotype of dengue virus (denv) is a major contributing factor for the induction of dengue hemorrhagic fever (dhf) and dengue shock syndrome (dss), two major medical conditions caused by denv infection. however, there is no specific drug or vaccine available against dengue infection. there are reports indicating the increased incidence of concurrent infec ...201728316881
frequency of aedes sp. linnaeus (diptera: culicidae) and associated entomofauna in bromeliads from a forest patch within a densely urbanized area.little is known about the relationship between the presence of aedes, abiotic factors and the entomofauna existing in phytotelmatas. the aim of this study was to identify biotic and abiotic factors associated with the presence of aedes in bromeliads sites located in a forest fragment in rio de janeiro, brazil. in the study area, eight bromeliads from the aechmea genus were chosen and kept in landscape form. physical and chemical variables were measured. collected aquatic insects were identified ...201728315189
a dynamic survival detection and analysis system for mosquito larvae viability in drug assays.bioinformatics studies have emerged in the domain of larval behavior analysis in recent years. a dynamic survival detection and analysis system for automatically monitoring a large amount of mosquito larvae in bioassays with multiwell plates by acquiring and processing videos is proposed in this article. in our system, equipment is designed for acquiring the video of the mosquito larvae in several multiwell plates simultaneously by a camera, and a video analysis module is developed for detecting ...201728314109
interspecific variation in desiccation survival time of aedes (stegomyia) mosquito eggs is correlated with habitat and egg size.survival times of eggs under three humidity conditions (42%, 68%, 88% rh) were investigated among aedes (stegomyia) mosquitoes from temperate and tropical zones (5 species and 20 geographical strains). this subgenus tends to occupy small aquatic sites as larvae, where desiccation resistance of eggs is necessary during habitat drought. interspecific comparison showed that the egg survival time was correlated with egg volume and dryness of source locality, and probably with habitat. aedes aegypti ...199228313521
sublethal effects of iridovirus disease in a mosquito.recognition of the importance of debilitating effects of insect virus diseases is currently growing. commonly observed effects of sublethal infection at the individual level include extended development times, reduced pupal and adult weights, and lowered fecundity. however, for the most part, sublethal infections are assumed to be present in survivors of an inoculum challenge, rather than demonstrated to be present by microscopy or molecular techniques. invertebrate iridescent viruses are dsdna ...199928307761
toxicity of spinosad to temephos-resistant aedes aegypti populations in brazil.the mosquito aedes aegypti is the primary vector of different arboviruses and represents a major public health problem. several brazilian populations of ae. aegypti have developed resistance to temephos, the most used organophosphate larvicide. new tools which are less harmful to the environment and safer for humans are becoming increasingly important to control this insect vector. spinosad, an aerobic fermentation product of a soil actinobacteria, has a favorable environmental profile. it prese ...201728301568
evaluation of reference genes at different developmental stages for quantitative real-time pcr in aedes aegypti.the mosquito aedes aegypti (ae. aegypti) is the most notorious vector of illness-causing viruses such as dengue, chikugunya, and zika. although numerous genetic expression studies utilizing quantitative real-time pcr (qpcr) have been conducted with regards to ae. aegypti, a panel of genes to be used suitably as references for the purpose of expression-level normalization within this epidemiologically important insect is presently lacking. here, the usability of seven widely-utilized reference ge ...201728300076
dengue virus ns2b/ns3 protease inhibitors exploiting the prime side.the mosquito-transmitted dengue virus (denv) infects millions of people in tropical and sub-tropical regions. maturation of denv particles requires proper cleavage of the viral polyprotein, including processing 8 of the 13 substrate cleavage sites by dengue ns2b-s3 protease. with no available direct-acting antiviral targeting denv, ns2-s3 protease is a promising target for inhibitor design. current design efforts focus on the nonprime side of the denv protease active site, resulting in highly hy ...201728298600
computer-aided structure based drug design approaches for the discovery of new anti-chikv agents.chikungunya is a viral infection caused by chikungunya virus (chikv), an arbovirus transmitted through mosquito (aedes aegypti and aedes albopictus) bite. the virus from sylvatic cycle in africa mutated to new vector adaptation and became one of the major emerging and re-emerging viral infections in the past decade, affecting more than 40 countries. efforts are being made by many researches to develop means to prevent and control the infection through vaccines and vector control strategy. on the ...201728294048
correction: laboratory strains of aedes aegypti are competent to brazilian zika virus.[this corrects the article doi: 10.1371/journal.pone.0171951.].201728288192
variation in aedes aegypti mosquito competence for zika virus transmission.to test whether zika virus has adapted for more efficient transmission by aedes aegypti mosquitoes, leading to recent urban outbreaks, we fed mosquitoes from brazil, the dominican republic, and the united states artificial blood meals containing 1 of 3 zika virus strains (senegal, cambodia, mexico) and monitored infection, dissemination, and virus in saliva. contrary to our hypothesis, cambodia and mexica strains were less infectious than the senegal strain. only mosquitoes from the dominican re ...201728287375
<i>aedes aegypti</i> control through modernized, integrated vector management.in the context of the ongoing, unprecedented zika virus outbreak in the americas, the world health organization has expressed its support for developing and up-scaling three novel approaches to controlling the aedes aegypti mosquito: the sterile insect technique (sit), the release of insects carrying dominant lethal genes (ridl) and the release of wolbachia-infected mosquitoes. whereas the former two approaches are temporary insect population suppression strategies, wolbachia infection is a self ...201728286698
longitudinal study of thai people media exposure, knowledge, and behavior on dengue fever prevention and control.dengue hemorrhagic fever is transmitted through a bite by a dengue -infected aedes aegypti mosquito. it was first reported in the mid -20th century in thailand, and since then its epidemiology has been of great concern and has spread all across the country. the alarming incidence of dengue posed a serious threat to human health in all major cities of thailand. this study was aimed at identifying the level of awareness of dengue fever in thai population knowledge for prevention and control, and m ...201728285970
repellency of the origanum onites l. essential oil and constituents to the lone star tick and yellow fever mosquito.the oregano, origanum onites l., essential oil (eo) was tested in laboratory behavioural bioassays for repellent activity against amblyomma americanum (l.) and aedes aegypti (l.). the o. onites eo was characterised using gc-fid and gc-ms. carvacrol (75.70%), linalool (9.0%), p-cymene (4.33%) and thymol (1.9%) were the most abundant compounds. at a concentration of 0.413 mg oil/cm(2) of filter paper, o. onites eo repelled 100% of the ticks tested and at 0.103 mg oil/cm(2) of filter paper, 66.7% o ...201728278656
vertical transmission of zika virus by aedes aegypti and ae. albopictus mosquitoes.to determine the potential role of vertical transmission in zika virus expansion, we evaluated larval pools of perorally infected aedes. aegypti and ae. albopictus adult female mosquitoes; ≈1/84 larvae tested were zika virus-positive; and rates varied among mosquito populations. thus, vertical transmission may play a role in zika virus spread and maintenance.201728277199
mosquito-borne diseases and omics: salivary gland proteome of the female aedes aegypti mosquito.the female aedes aegypti mosquito is an important vector for several tropical and subtropical diseases such as dengue, chikungunya, and zika and yellow fever. the disease viruses infect the mosquito and subsequently spread to the salivary glands after which the viruses can be transmitted to humans with probing or feeding by the mosquito. omics systems sciences offer the opportunity to characterize vectors and can inform disease surveillance, vector control and development of innovative diagnosti ...201728271980
the microbiome composition of aedes aegypti is not critical for wolbachia-mediated inhibition of dengue virus.dengue virus (denv) is primarily vectored by the mosquito aedes aegypti, and is estimated to cause 390 million human infections annually. a novel method for denv control involves stable transinfection of ae. aegypti with the common insect endosymbiont wolbachia, which mediates an antiviral effect. however, the mechanism by which wolbachia reduces the susceptibility of ae. aegypti to denv is not fully understood. in this study we assessed the potential of resident microbiota, which can play impor ...201728267749
cre/lox-recombinase-mediated cassette exchange for reversible site-specific genomic targeting of the disease vector, aedes aegypti.site-specific genome modification (ssm) is an important tool for mosquito functional genomics and comparative gene expression studies, which contribute to a better understanding of mosquito biology and are thus a key to finding new strategies to eliminate vector-borne diseases. moreover, it allows for the creation of advanced transgenic strains for vector control programs. ssm circumvents the drawbacks of transposon-mediated transgenesis, where random transgene integration into the host genome r ...201728266580
chemicals isolated from justicia adhatoda linn reduce fitness of the mosquito, aedes aegypti l.extracts from justicia adhatoda l. (acanthaceae) strongly reduced the fitness of the mosquito, aedes aegypti linn. the methanolic extracts inhibited several enzymes responsible for protecting insects from oxidative and other damage, including glutathione-s-transferase, superoxide dismutase, cytochrome p450, and α- and β-esterases. they increased repellency (maximum repellency at 100 ppm) in host-seeking adult females using the "arm-in cage assay." histopathological examination showed the extract ...201728266058
the rnai pathway plays a small part in wolbachia-mediated blocking of dengue virus in mosquito cells.wolbachia pipientis is an insect endosymbiont known to limit the replication of viruses including dengue and zika in their primary mosquito vector, aedes aegypti. wolbachia is being released into mosquito populations globally in a bid to control the diseases caused by these viruses. it is theorized that wolbachia's priming of the insect immune system may confer protection against subsequent viral infection. other hypotheses posit a role for competition between wolbachia and viruses for host cell ...201728262718
comparative luciferase assay for establishing reliable in vitro screening system of juvenile hormone agonists.the cultured cell-based in vitro assay using the stringency of ligand-receptor interactions is typically useful for screening certain hormone agonists from among a very large number of molecules. however, ligands are frequently altered or modified through evolution; indeed, even in the same receptor orthologs, different ligand sensitivity profiles are considered to arise among species and/or taxa. such ligand transition has been observed in juvenile hormone (jh), one of the most important endocr ...201728261832
blood feeding status, gonotrophic cycle and survivorship of aedes (stegomyia) aegypti (l.) (diptera: culicidae) caught in churches from merida, yucatan, mexico.blood-feeding status, gonotrophic cycle, and survival rates of aedes (stegmyia) aegypti (l.) was investigated in catholic churches from merida, yucatan. female ae. aegypti were caught using backpack aspirator during 25 consecutive days in rainy (2015) and dry season (2016). blood-feeding status was determined by external examination of the abdomen and classified as unfed, fed, and gravid. daily changes in the parous-nulliparous ratio were recorded, and the gonotrophic cycle length was estimated ...201728258352
insecticide susceptibility status and major detoxifying enzymes' activity in aedes albopictus (skuse), vector of dengue and chikungunya in northern part of west bengal, india.mosquitoes belonging to aedes genus, aedes aegypti and aedes albopictus transmit many globally important arboviruses including dengue (denv) and chikungunya (chikv). vector control with the use of insecticide remains the suitable method of choice to stop the transmission of these diseases. however, vector control throughout the world is failing to achieve its target results because of the worldwide development of insecticide resistance in mosquitoes. to assess the insecticide susceptibility stat ...201728254583
mosgctl-7, a c-type lectin protein, mediates japanese encephalitis virus infection in mosquitoes.japanese encephalitis virus (jev) is an arthropod-borne flavivirus prevalent in asia and the western pacific, and is the leading cause of viral encephalitis. jev is maintained in a transmission cycle between mosquitoes and vertebrate hosts, but the molecular mechanisms by which the mosquito vector participates in transmission are unclear. we investigated the expression of all c-type lectins during jev infection in aedes aegypti. c-type lectin mosgctl-7 (aael002524) was significantly up-regulated ...201728250133
a mesh-duox pathway regulates homeostasis in the insect gut.the metazoan gut harbours complex communities of commensal and symbiotic bacterial microorganisms. the quantity and quality of these microorganisms fluctuate dynamically in response to physiological changes. the mechanisms that hosts have developed to respond to and manage such dynamic changes and maintain homeostasis remain largely unknown. here, we identify a dual oxidase (duox)-regulating pathway that contributes to maintaining homeostasis in the gut of both aedes aegypti and drosophila melan ...201728248301
population genomics reveals that an anthropophilic population of aedes aegypti mosquitoes in west africa recently gave rise to american and asian populations of this major disease vector.the mosquito aedes aegypti is the main vector of dengue, zika, chikungunya and yellow fever viruses. this major disease vector is thought to have arisen when the african subspecies ae. aegypti formosus evolved from being zoophilic and living in forest habitats into a form that specialises on humans and resides near human population centres. the resulting domestic subspecies, ae. aegypti aegypti, is found throughout the tropics and largely blood-feeds on humans.201728241828
zika in rio de janeiro: assessment of basic reproduction number and comparison with dengue outbreaks.zika virus infection was declared a public health emergency of international concern in february 2016 in response to the outbreak in brazil and its suspected link with congenital anomalies. in this study, we use notification data and disease natural history parameters to estimate the basic reproduction number (r 0) of zika in rio de janeiro, brazil. we also obtain estimates of r 0 of dengue from time series of dengue cases in the outbreaks registered in 2002 and 2012 in the city, when denv-3 and ...201728240195
allergenome characterization of the mosquito aedes aegypti.saliva and muscle derived mosquito allergens have been purified and characterized. however, the complete set of allergens remains to be elucidated. in this study, we identified and characterized ige-binding proteins from the mosquito species a. aegypti.201728235135
chikungunya infection: a global public health menace.chikungunya virus (chikv) has been involved in epidemics in african and asian subcontinents and, of late, has transcended to affect the americas. aedes aegypti and aedes albopictus are the major vectors for chikv infection, which results in dissemination of virus to various vital organs. entry of virus into these tissues causes infiltration of innate immune cells, monocytes, macrophages, neutrophils, natural killer cells, and adaptive immune cells. macrophages bearing the replicating virus, in t ...201728233156
combining contact tracing with targeted indoor residual spraying significantly reduces dengue transmission.the widespread transmission of dengue viruses (denv), coupled with the alarming increase of birth defects and neurological disorders associated with zika virus, has put the world in dire need of more efficacious tools for aedes aegypti-borne disease mitigation. we quantitatively investigated the epidemiological value of location-based contact tracing (identifying potential out-of-home exposure locations by phone interviews) to infer transmission foci where high-quality insecticide applications c ...201728232955
microbiota activates imd pathway and limits sindbis infection in aedes aegypti.aedes aegypti is the main vector of important arboviruses such as dengue, zika and chikungunya. during infections mosquitoes can activate the immune pathways toll, imd and jak/stat to limit pathogen replication.201728231846
characterization of carbonic anhydrase 9 in the alimentary canal of aedes aegypti and its relationship to homologous mosquito carbonic anhydrases.in the mosquito midgut, luminal ph regulation and cellular ion transport processes are important for the digestion of food and maintenance of cellular homeostasis. ph regulation in the mosquito gut is affected by the vectorial movement of the principal ions including bicarbonate/carbonate and protons. as in all metazoans, mosquitoes employ the product of aerobic metabolism carbon dioxide in its bicarbonate/carbonate form as one of the major buffers of cellular and extracellular ph. the conversio ...201728230813
relish2 mediates bursicon homodimer-induced prophylactic immunity in the mosquito aedes aegypti.bursicon is a neuropeptide hormone consisting of two cystine-knot proteins (burs α and burs β), responsible for cuticle tanning and other developmental processes in insects. recent studies show that each bursicon subunit forms homodimers that induce prophylactic immunity in drosophila melanogaster. here, we investigated the hypothesis that bursicon homodimers act in prophylactic immunity in insects, and possibly arthropods, generally, using the mosquito, aedes aegypti. we found that burs α and b ...201728225068
transcriptome-wide microrna and target dynamics in the fat body during the gonadotrophic cycle of aedes aegypti.the mosquito aedes aegypti is a major vector of numerous viral diseases, because it requires a blood meal to facilitate egg development. the fat body, a counterpart of mammalian liver and adipose tissues, is the metabolic center, playing a key role in reproduction. therefore, understanding of regulatory networks controlling its functions is critical, and the role of micrornas (mirnas) in the process is largely unknown. we aimed to explore mirna expression and potential targets in the female fat ...201728223504
development of dirofilaria immitis and dirofilaria repens in aedes japonicus and aedes geniculatus.the mosquito-borne filarial nematodes dirofilaria immitis and dirofilaria repens primarily affect dogs but also cats, causing heartworm disease or subcutaneous dirofilariosis, respectively, and both may also cause zoonotic diseases in humans. several mosquito species have been reported as competent vectors for these nematodes, but no data are available for the invasive mosquito species aedes japonicus (theobald, 1901). the objective of this study was to describe the development of both d. immiti ...201728219407
regulation of antimicrobial peptides in aedes aegypti aag2 cells.antimicrobial peptides (amps) are an important group of immune effectors that play a role in combating microbial infections in invertebrates. most of the current information on the regulation of insect amps in microbial infection have been gained from drosophila, and their regulation in other insects are still not completely understood. here, we generated an amp induction profile in response to infections with some gram-negative, -positive bacteria, and fungi in aedes aegypti embryonic aag2 cell ...201728217557
comparative analysis of mosquito (diptera: culicidae: aedes aegypti liston) responses to the insecticide temephos and plant derived essential oil derived from piper betle l.resistance to treatments with temephos or plant derived oil, pb-cvo, between a field collected wild strain (ws) and a susceptible laboratory strain (ls) of ae. aegypti were measured. the temephos (0.1mg/l) showed the greatest percentage of mosquito mortality compared to pb-cvo (1.5mg/l) in ls ae. aegypti. however, ws ae. aegypti was not significantly affected by temephos (0.1mg/l) treatment compare to the pb-cvo (1.5mg/l). however, both strains (ls and ws) when treated with pb-cvo (1.5mg/l) disp ...201728213320
household dengue prevention interventions, expenditures, and barriers to aedes aegypti control in machala, ecuador.the aedes aegypti mosquito is an efficient vector for the transmission of zika, chikungunya, and dengue viruses, causing major epidemics and a significant social and economic burden throughout the tropics and subtropics. the primary means of preventing these diseases is household-level mosquito control. however, relatively little is known about the economic burden of ae. aegypti control in resource-limited communities. we surveyed residents from 40 households in a high-risk community at the urba ...201728212349
dengue: an analysis of epidemiological pattern over a six year period.dengue is an arboviral infection transmitted among humans by aedes aegypti, the urban vector and aedes albopictus as a maintenance vector in sub-urban and rural areas. the diagnosis is routinely established by detection of ns1 antigen and/or by igm antibodies testing. dengue infects 50-100 million cases per year worldwide with atleast half million serious cases needing hospitalization. therefore, it is necessary to keep the surveillance ongoing to timely look out for changes occurring in disease ...201628208855
leaking containers: success and failure in controlling the mosquito aedes aegypti in brazil.in 1958, the pan american health organization declared that brazil had successfully eradicated the mosquito aedes aegypti, responsible for the transmission of yellow fever, dengue fever, chikungunya, and zika virus. yet in 2016 the brazilian minister of health described the situation of dengue fever as "catastrophic." discussing the recent epidemic of zika virus, which amplified the crisis produced by the persistence of dengue fever, brazil's president declared in january 2016 that "we are in th ...201728207332
acoustic control of mosquito larvae in artificial drinking water containers.emerging technology designed to kill mosquito larvae with sound waves may present a nonchemical and nonbiological alternative to reduce larval populations of key medically important mosquito species such as aedes aegypti in containers or catchments of water. these devices could benefit integrated vector management programs facing public resistance to the use of chemical or biological larvicides in stored drinking water. in this study we investigate the efficacy of a larvasonic sd-mini acoustic l ...201628206869
effect of photoperiod on permethrin resistance in aedes aegypti.living organisms have been exposed to light-dark cycles that allowed them to adapt to different ecological niches. circadian cycles affect hormone release, metabolism, and response to xenobiotic compounds. current studies have shown that insect susceptibility to toxic agents depends on circadian cycles, mainly because the biochemical processes involved in detoxification and responses to oxidative stress are modulated by this process. the goal of this study was to determine the effect of photoper ...201628206856
does zika virus infection affect mosquito response to repellents?the world health organization (who) recommends that people travelling to or living in areas with zika virus (zikv) outbreaks or epidemics adopt prophylactic measures to reduce or eliminate mosquito bites, including the use of insect repellents. it is, however, unknown whether repellents are effective against zikv-infected mosquitoes, in part because of the ethical concerns related to exposing a human subject's arm to infected mosquitoes in the standard arm-in-cage assay. we used a previously dev ...201728205633
bombyx mori and aedes aegypti form multi-functional immune complexes that integrate pattern recognition, melanization, coagulants, and hemocyte recruitment.the innate immune system of insects responds to wounding and pathogens by mobilizing multiple pathways that provide both systemic and localized protection. key localized responses in hemolymph include melanization, coagulation, and hemocyte encapsulation, which synergistically seal wounds and envelop and destroy pathogens. to be effective, these pathways require a targeted deposition of their components to provide protection without compromising the host. extensive research has identified a larg ...201728199361
a large scale biorational approach using bacillus thuringiensis israeliensis (strain am65-52) for managing aedes aegypti populations to prevent dengue, chikungunya and zika transmission.aedes aegypti is a container-inhabiting mosquito and a vector of dengue, chikungunya, and zika viruses. in 2009 several cases of autochthonous dengue transmission were reported in key west, florida, usa prompting a comprehensive response to control a. aegypti. in key west, larvae of this mosquito develop in containers around human habitations which can be numerous and labor intensive to find and treat. aerial applications of larvicide covering large areas in a short time can be an efficient and ...201728199323
a cdna clone-launched platform for high-yield production of inactivated zika vaccine.a purified inactivated vaccine (piv) using the zika virus (zikv) puerto rico strain prvabc59 showed efficacy in monkeys, and is currently in a phase i clinical trial. high-yield manufacture of this piv is essential for its development and vaccine access. here we report an infectious cdna clone-launched platform to maximize its yield. a single ns1 protein substitution (k265e) was identified to increase zikv replication on vero cells (a cell line approved for vaccine production) for both cambodian ...201728196656
meteorological variables and mosquito monitoring are good predictors for infestation trends of aedes aegypti, the vector of dengue, chikungunya and zika.aedes aegypti is an important vector for arboviroses and widely distributed throughout the world. climatic factors can influence vector population dynamics and, consequently, disease transmission. the aim of this study was to characterize the temporal dynamics of an ae. aegypti population and dengue cases and to investigate the relationship between meteorological variables and mosquito infestation.201728193291
prevalence of dengue and diversity of cultivable bacteria in vector aedes aegypti (l.) from two dengue endemic districts, kanchanpur and parsa of nepal.dengue fever, an endemic arboviral disease, represents one of the major public health concerns in nepal. it is transmitted by bites of infected aedes aegypti and aedes albopictus, the former being primary vector. the bacterial community plays a significant role in biology of mosquitoes; however, the bacterial communities of primary vector a. aegypti remain unstudied in nepal. the study was designed to determine the rate of dengue seropositivity and to explore the bacterial diversity of a. aegypt ...201728193261
aedes-borne virus-mosquito interactions: mass spectrometry strategies and findings.aedes-borne viruses are responsible for high-impact neglected tropical diseases and unpredictable outbreaks such as the ongoing zika epidemics. aedes mosquitoes spread different arboviruses such as dengue virus (denv), chikungunya virus (chikv), and zika virus, among others, and are responsible for the continuous emergence and reemergence of these pathogens. these viruses have complex transmission cycles that include two hosts, namely the aedes mosquito as a vector and susceptible vertebrate hos ...201728192064
leptolegnia chapmanii (straminipila: peronosporomycetes) as a future biorational tool for the control of aedes aegypti (l.).the aim of the present review is to summarize the current knowledge about leptolegnia chapmanii as a pathogen of mosquito larvae. to this end, we present data on its identification, distribution, host range and effects on non-target organisms, effects of environmental factors, in vitro growth, release and persistence in anthropic environments, and effect combined with other insecticides. the data presented allow confirming its potential as a biocontrol agent.201728188766
levels of insecticide resistance to deltamethrin, malathion, and temephos, and associated mechanisms in aedes aegypti mosquitoes from the guadeloupe and saint martin islands (french west indies).in the guadeloupe and saint martin islands, aedes aegypti mosquitoes are the only recognized vectors of dengue, chikungunya, and zika viruses. for around 40 years, malathion was used as a mosquito adulticide and temephos as a larvicide. since the european union banned the use of these two insecticide molecules in the first decade of the 21st century, deltamethrin and bacillus thuringiensis var. israelensis are the remaining adulticide and larvicide, respectively, used in guadeloupe. in order to ...201728187780
zika and chikungunya: mosquito-borne viruses in a changing world.the reemergence and growing burden of mosquito-borne virus infections have incited public fear and growing research efforts to understand the mechanisms of infection-associated health outcomes and to provide better approaches for mosquito vector control. while efforts to develop therapeutics, vaccines, and novel genetic mosquito-control technologies are underway, many important underlying ecological questions remain that could significantly enhance our understanding and ability to predict and pr ...201728187236
laboratory strains of aedes aegypti are competent to brazilian zika virus.the zika virus outbreaks are unprecedented human threat in relation to congenital malformations and neurological/autoimmune complications. since this virus has high potential to spread in regions presenting the vectors, improvement in mosquito control is a top priority. thus, aedes aegypti laboratory strains will be fundamental to support studies in different research fields implicated on zika-mosquito interactions which are the basis for the development of innovative control methods. in this se ...201728187183
aedes aegypti larvicidal sesquiterpene alkaloids from maytenus oblongata.four new sesquiterpene alkaloids (1-4) with a β-dihydroagrofuran skeleton and a new triterpenoid (5) were isolated from an ethyl acetate extract of maytenus oblongata stems. their structures were elucidated using 1d and 2d nmr spectroscopy as well as ms and ecd experiments. the m. oblongata stem etoac extract and the pure compounds isolated were tested for larvicidal activity against aedes aegypti under laboratory conditions, and compounds 2 and 3 were found to be active.201728186749
zika virus and neuroscience: the need for a translational collaboration.zika virus (zikv) has become a major challenge for scientists and health agencies. zikv's involvement with human fetal microcephaly and guillain-barré syndrome and its transmission through aedes africanus and aedes aegypti mosquitos highlighted the epidemiological and neurological risks associated to zikv infection. in 2013, zikv arrives in brazil but the first outbreak in the country was reported in 2015. here, we used the web of science as a search tool for comparing the evolution of world and ...201728185126
environmental and social change drive the explosive emergence of zika virus in the americas.since zika virus (zikv) was detected in brazil in 2015, it has spread explosively across the americas and has been linked to increased incidence of microcephaly and guillain-barré syndrome (gbs). in one year, it has infected over 500,000 people (suspected and confirmed cases) in 40 countries and territories in the americas. along with recent epidemics of dengue (denv) and chikungunya virus (chikv), which are also transmitted by aedes aegypti and ae. albopictus mosquitoes, the emergence of zikv s ...201728182667
single molecule rna sequencing uncovers trans-splicing and improves annotations in anopheles stephensi.single molecule real-time (smrt) sequencing has recently been used to obtain full-length cdna sequences that improve genome annotation and reveal rna isoforms. here, we used one such method called isoform sequencing from pacific biosciences (pacbio) to sequence a cdna library from the asian malaria mosquito anopheles stephensi. more than 600 000 full-length cdnas, referred to as reads of insert, were identified. owing to the inherently high error rate of pacbio sequencing, we tested different ap ...201728181326
predicting the potential role of non-human hosts in zika virus maintenance.arboviruses are often maintained in complex cycles involving vertebrates such as mammals or birds and blood-feeding mosquitoes. however, the role of wildlife hosts in their emergence or re-emergence in human populations has received little attention. the recent emergence of zika virus in america, and previous occurrences of chikungunya and dengue, forces us to confront a potential new disease-emergence phenomenon. using a spatial data mining framework to identify potential biotic interactions, b ...201728180996
xanthine dehydrogenase-1 silencing in aedes aegypti mosquitoes promotes a blood feeding-induced adulticidal activity.aedesaegypti has 2 genes encoding xanthine dehydrogenase (xdh). we analyzed xdh1 and xdh2 gene expression by real-time quantitative pcr in tissues from sugar- and blood-fed females. differential xdh1 and xdh2 gene expression was observed in tissues dissected throughout a time course. we next exposed females to blood meals supplemented with allopurinol, a well-characterized xdh inhibitor. we also tested the effects of injecting double-stranded rna (dsrna) against xdh1, xdh2, or both. disruption o ...201728179423
severe chikungunya infection in northern mozambique: a case report.although chikungunya virus has rapidly expanded to several countries in sub-saharan africa, little attention has been paid to its control and management. until recently, chikungunya has been regarded as a benign and self-limiting disease. in this report we describe the first case of severe chikungunya disease in an adult patient in pemba, mozambique.201728179029
mosquitocidal activity and mode of action of the isoxazoline fluralaner.mosquitoes, such as aedes aegypti and anopheles gambiae, are important vectors of human diseases. fluralaner, a recently introduced parasiticide, was evaluated as a mosquitocide in this study. on ae. aegypti and an. gambiae fourth-instar larvae, fluralaner had 24-h lc50 (lethal concentration for 50% mortality) values of 1.8 ppb and 0.4 ppb, respectively. following topical application to adult ae. aegypti, fluralaner toxicity reached a plateau in about 3 days, with 1- and 3-day ld50 (lethal dose ...201728178191
sweet waste extract uptake by a mosquito vector: survival, biting, fecundity responses, and potential epidemiological significance.in nature, adult mosquitoes typically utilize nectar as their main energy source, but they can switch to other as yet unidentified sugary fluids. contemporary lifestyles, with their associated unwillingness to consume leftovers and improper disposal of waste, have resulted in the disposal of huge amounts of waste into the environment. such refuse often contains unfinished food items, many of which contain sugar and some of which can collect water from rain and generate juices. despite evidence t ...201728174057
reducing biting rates of aedes aegypti with metofluthrin: investigations in time and space.indoor residual spraying is key to dengue control in cairns and other parts of northern queensland, australia, where aedes aegypti is prevalent, but the strategy faces challenges with regards to slow application time and, therefore, community coverage. a faster potential improvement might be the use of polyethylene netting impregnated with the volatile pyrethroid metofluthrin (sumione™). this formulation was assessed in rooms in three houses in cairns, australia. one emanator was placed in each ...201728173824
larvicidal activity of blumea eriantha essential oil and its components against six mosquito species, including zika virus vectors: the promising potential of (4e,6z)-allo-ocimene, carvotanacetone and dodecyl acetate.the effective and environmentally sustainable control of mosquitoes is a challenge of essential importance. this is due to the fact that some invasive mosquitoes, with special reference to the aedes genus, are particularly difficult to control, due to their high ecological plasticity. moreover, the indiscriminate overuse of synthetic insecticides resulted in undesirable effects on human health and non-target organisms, as well as resistance development in targeted vectors. here, the leaf essenti ...201728168560
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