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laboratory and semi-field evaluations of two (transfluthrin) spatial repellent devices against aedes aegypti (l.) (diptera: culicidae).two transfluthrin-based spatial repellent products (raid dual action insect repellent and home freshener and raid shield (currently not commercially available), sc johnson, racine wi) were evaluated for spatial repellent effects against female aedes aegypti (l.) mosquitoes under laboratory (wind tunnel) and semi-field (outdoor enclosure) conditions. the placement of either product in the wind tunnel significantly reduced host-seeking behaviors. the mean baseline (control) landing counts for the ...201728511270
reemergence of yellow fever in ethiopia after 50 years, 2013: epidemiological and entomological investigations.yellow fever (yf) is a viral hemorrhagic disease transmitted by aedes mosquito species. approximately, 200,000 cases and 30,000 deaths occur worldwide every year. in ethiopia, the last outbreak was reported in 1966 with 2200 cases and 450 deaths. a number of cases with deaths from unknown febrile illness reported from south ari district starting from november 2012. this investigation was conducted to identify the causative agent, source of the outbreak and recommend appropriate interventions.201728506254
aedes aegypti (l.) mosquitoes in trinidad, west indies: longevity case studies.few laboratory and field studies have reported long survival periods for ae. aegypti females and even fewer have designed experiments to characterize this important life history trait. this study was conducted under laboratory conditions to determine the number of blood meals taken by individual females, the number of eggs laid per individual female, the length of the gonotrophic cycle, and the duration of female survival. the results showed individual females oviposited between 670 and 1,500 eg ...201728504438
influence of shading and pedestrian traffic on the preference of aedes (stegomyia) aegypti (diptera: culicidae) for oviposition microenvironments.aedes aegypti mosquitoes are highly adaptable to abiotic stimuli. to evaluate the influence of shading and pedestrian traffic on the preference of ae. aegypti for oviposition microenvironments, 20 sites were sampled weekly using ovitraps within the perimeter of universidade federal do espírito santo, located in são mateus, espírito santo, brazil. a spatial and statistical analysis was performed in order to assess the relationship between shading time, pedestrian traffic, and the presence of biol ...201728504433
isolation of an insulin-like peptide from the asian malaria mosquito, anopheles stephensi, that acts as a steroidogenic gonadotropin across diverse mosquito taxa.many insulin-like peptides (ilps) have been identified in insects, yet only a few were isolated in their native form for structural and functional studies. antiserum produced to ilp3 in aedes aegypti was used in a radioimmunoassay to monitor the purification of an ilp from heads of adult an. stephensi and recognized the ilp in other immunoassays. the structure of the purified peptide matched that predicted for the ilp3 in this species. the native form stimulated ecdysteroid production by ovaries ...201728502740
metazoan ribosome inactivating protein encoding genes acquired by horizontal gene transfer.ribosome inactivating proteins (rips) are rna n-glycosidases that depurinate a specific adenine residue in the conserved sarcin/ricin loop of 28s rrna. these enzymes are widely distributed among plants and their presence has also been confirmed in several bacterial species. recently, we reported for the first time in silico evidence of rip encoding genes in metazoans, in two closely related species of insects: aedes aegypti and culex quinquefasciatus. here, we have experimentally confirmed the p ...201728500327
larval interference competition between the native neotropical mosquito limatus durhamii and the invasive aedes aegypti improves the fitness of both species.interspecific competition with native species during biological invasions can sometimes limit alien expansion. we aimed to determine the potential ecological effects of limatus durhamii theobald 1901, a native neotropical mosquito (diptera: culicidae) species, on the invasive species aedes (stegomyia) aegypti (linnaeus 1762) that breeds in the same artificial water containers. development time and adult dry mass were measured in 3 rearing conditions: control (a single larva), intraspecific compe ...201728497885
assessing the effect of selection with deltamethrin on biological parameters and detoxifying enzymes in aedes aegypti (l.).resistance to insecticides through one or several mechanisms has a cost for an insect in various parameters of its biological cycle. the present study evaluated the effect of deltamethrin on detoxifying enzymes and biological parameters in a population of aedes aegypti selected for 15 generations. the enzyme activities of alpha- and beta-esterases, mixed-function oxidases and glutathione-s-transferases were determined during selection, along with biological parameters.201728485040
what does heat tell a mosquito? characterization of the orientation behaviour of aedes aegypti towards heat sources.the use of heat as a cue for the orientation of haematophagous insects towards hot-blooded hosts has been acknowledged for many decades. in mosquitoes, thermoreception has been studied at the molecular, physiological and behavioural levels, and the response to heat has been evaluated in multimodal contexts. however, a direct characterization of how these insects evaluate thermal sources is still lacking. in this study we characterize aedes aegypti thermal orientation using a simple dual choice p ...201728479122
eco-friendly synthesis of silver nanoparticles and its larvicidal property against fourth instar larvae of aedes aegypti.in this study, larvicidal activity of silver nanoparticles (agnps) synthesised using apple extract against fourth instar larvae of aedes aegypti was determined. as a result, the agnps showed moderate larvicidal effects against ae. aegypti larvae (lc50 = 15.76 ppm and lc90 = 27.7 ppm). in addition, comparison of larvicidal activity performance of agnps at high concentration prepared using two different methods showed that ae. aegypti larvae was fully eliminated within the duration of 2.5 h. from ...201728476997
photo-induced and phytomediated synthesis of silver nanoparticles using derris trifoliata leaf extract and its larvicidal activity against aedes aegypti.due to the increased development of resistance of vectors against synthetic insecticides and chemical drugs, plant based insecticides serve as promising biocontrol agents for effective vector control. green approach for the synthesis of nanoparticles has been attained using environmentally safe, non-toxic plant extracts. the present study was aimed to investigate the potent larvicidal activity of silver nanoparticles (agnps) produced by derris trifoliata leaf extract in relation to the various c ...201728460330
allergens involved in the cross-reactivity of aedes aegypti with other arthropods.cross-reactivity between aedes aegypti and mites, cockroaches, and shrimp has been previously suggested, but the involved molecular components have not been fully described.201728434865
n-aryl-n'-ethyleneaminothioureas effectively inhibit acetylcholinesterase 1 from disease-transmitting mosquitoes.vector control of disease-transmitting mosquitoes by insecticides has a central role in reducing the number of parasitic- and viral infection cases. the currently used insecticides are efficient, but safety concerns and the development of insecticide-resistant mosquito strains warrant the search for alternative compound classes for vector control. here, we have designed and synthesized thiourea-based compounds as non-covalent inhibitors of acetylcholinesterase 1 (ache1) from the mosquitoes anoph ...201728433681
identification of the septate junction protein gliotactin in the mosquito aedes aegypti: evidence for a role in increased paracellular permeability in larvae.septate junctions (sjs) regulate paracellular permeability across invertebrate epithelia. however, little is known about the function of sj proteins in aquatic invertebrates. in this study, a role for the transmembrane sj protein gliotactin (gli) in the osmoregulatory strategies of larval mosquito (aedes aegypti) was examined. differences in gli transcript abundance were observed between the midgut, malpighian tubules, hindgut and anal papillae of a. aegypti, which are epithelia that participate ...201728432154
allatotropin: a pleiotropic neuropeptide that elicits mosquito immune responses.allatotropins (at) are neuropeptides with pleotropic functions on a variety of insect tissues. they affect processes such as juvenile hormone biosynthesis, cardiac rhythm, oviduct and hindgut contractions, nutrient absorption and circadian cycle. the present work provides experimental evidence that at elicits immune responses in two important mosquito disease vectors, anopheles albimanus and aedes aegypti. hemocytes and an immune-competent mosquito cell line responded to at by showing strong mor ...201728426765
efficacy of some wearable devices compared with spray-on insect repellents for the yellow fever mosquito, aedes aegypti (l.) (diptera: culicidae).the current zika health crisis in the americas has created an intense interest in mosquito control methods and products. mosquito vectors of zika are of the genus aedes, mainly the yellow fever mosquito, aedes aegypti. l. the use of repellents to alter mosquito host seeking behavior is an effective method for the prevention of mosquito-borne diseases. a large number of different spray-on repellents and wearable repellent devices are commercially available. the efficacies of many repellents are u ...201728423421
habitat and density of oviposition opportunity influences aedes aegypti (diptera: culicidae) flight distance.understanding the dispersal of aedes (aegypti (l.) diptera: culicidae) after consuming a potentially infectious bloodmeal is an important part of controlling the spread of the arboviruses it transmits. because of the impact on abundance, removal of oviposition sites is a key component of vector control. however, source reduction around a case may encourage dispersal of potentially infected vectors. we compare the effect of oviposition site availability on ae. aegypti dispersal behavior within 30 ...201728419273
the impact of temperature and body size on fundamental flight tone variation in the mosquito vector aedes aegypti (diptera: culicidae): implications for acoustic lures.aedes aegypti (l.) males use female flight tone as a means of mate localization. by playing the sound of a flying female, males can be attracted to a trap to monitor mosquito populations and the progress of transgenic male releases. however, the female flight tone used to attract males needs to be optimized to maximize trap effectiveness. the fundamental frequency of female flight tone could be influenced by both body size and ambient temperature. however, no analysis yet has considered both the ...201728402550
immunohistochemical mapping and transcript expression of the gpa2/gpb5 receptor in tissues of the adult mosquito, aedes aegypti.gpa2/gpb5 is a glycoprotein hormone found in most bilateral metazoans including the mosquito, aedes aegypti. to elucidate physiological roles and functions of gpa2/gpb5, we aim to identify prospective target tissues by examining the tissue- and sex-specific expression profile of its receptor, the leucine-rich repeat-containing g protein-coupled receptor 1 (lgr1) in the adult mosquito. western analyses using a heterologous system with cho-k1 cells, transiently expressing a. aegypti lgr1, yielded ...201728401307
full-genome dengue virus sequencing in mosquito saliva shows lack of convergent positive selection during transmission byaedes aegypti.like other pathogens with high mutation and replication rates, within-host dengue virus (denv) populations evolve during infection of their main mosquito vector,aedes aegypti. within-host denv evolution during transmission provides opportunities for adaptation and emergence of novel virus variants. recent studies of denv genetic diversity failed to detect convergent evolution of adaptive mutations in mosquito tissues such as midgut and salivary glands, suggesting that convergent positive selecti ...201729497564
[zika: probability of establishment of its vector, aedes aegypti, in chile].the zika virus has raised world alarm in recent years, representing a major public health problem. in this study we evaluated the potential risk of exposure to zika virus in chile, associated with the probability of establishment of the vector aedes aegypti in the country. niche modelling techniques were used to project the bioclimatic requirements of the vector (global niches), identifying zones of high suitability for the species within the country. then, the potential distribution of the vect ...201729488548
[(assessment of risk for arbovirus infections in russia)].the sharply increased incidence and geographical expansion of arbovirus diseases in recent years suggest that some russian areas are vulnerable to vector-borne diseases and that it is important to elaborate control programs to ensure bio- logical safety in our country. moreover, the main vectors of the pathogens of arbovirus infections (zika fever, dengue fever, chikungunya fevers), such as aedes aegypti and ae.albopictus, have been registered on the black sea coast of the caucasus. the programs ...201730721608
larvicidal and pupicidal activities of crude and fractionated extracts of acacia pennata (l.) willd. subsp insuavis shoot tips against aedes aegypti (l.) (diptera: culicidae).acacia pennata subsp insuavis, or cha-om in thai, is a common vegetablefound in thailand. it has been used as a medicinal herb for a long time. from theliterature, antinociceptive, anti-inflammatory, antimicrobial, and anti-helminthicactivities were reported. in this study, we investigated two new actions of thisplant: larvicide and pupicide. the crude ethanolic and fractionated extracts of a.pennata shoot tips were tested against aquatic stages of the dengue virus vector,aedes aegypti mosquito. ...201729644817
[clinical and epidemiological profile of chikungunya infection in hospital patients in 2015 in mérida, mexicoperfil clínico-epidemiológico da infecção por chikungunya em casos hospitalares atendidos em 2015 em mérida, méxico].describe the epidemiological profile and analyze clinical manifestations by age group in chikungunya patients treated in 2015 in a mérida, mexico general hospital.201731391823
erratum: efficacy of some wearable devices compared with spray-on insect repellents for the yellow fever mosquito, aedes aegypti (l.) (diptera: culicidae). 201728365772
tropical viruses approaching europe.during the last years, headlines about the advance of tropical infectious diseases, which are transmitted by mosquitos, become more frequent. some of these mosquitos are classified as invasive species in europe. the world health organization (who) and the european center for disease prevention and control (ecdc) are enhancing the observation of the geographical spread of these species. especially the asian tiger mosquito and the yellow fever mosquito are in focus. in germany the vector competenc ...201729952164
tropical viruses approaching europe.during the last years, headlines about the advance of tropical infectious diseases, which are transmitted by mosquitos, become more frequent. some of these mosquitos are classified as invasive species in europe. the world health organization (who) and the european center for disease prevention and control (ecdc) are enhancing the observation of the geographical spread of these species. especially the asian tiger mosquito and the yellow fever mosquito are in focus. in germany the vector competenc ...201729952163
fly ash-based water dispersible powder formulation of bacillus thuringiensis var. israelensis: development & laboratory evaluation against mosquito immatures.bacillus thuringiensis var. israelensis (bti) formulations are presently being used for insect control. in this study, a water dispersible powder (wdp) formulation using fly ash (fa) as a carrier material was developed and studied for its activity against the larval stages of major mosquito vector species.201729664029
infectivity of chordodes nobilii larvae (gordiida: nematomorpha).the gordiids are freshwater representatives of the parasite phylum nematomorpha that function asa link between aquatic and terrestrial ecosystems. in recent years, different ecotoxicologic studies have beenmade with the south-american gordiid species, chordodes nobilii, that have demonstrated the capacity of thisgroup to act as a bioindicator of contamination. despite the gordiida’s ecologic relevance, further studies arestill needed to elucidate different aspects of the biology of the class, an ...201729465953
environmental factors can influence dengue reported cases.global climate changes directly affect the natural environment and contribute to an increase in the transmission of diseases by vectors. among these diseases, dengue is at the top of the list. the aim of our study was to understand the consequences of temporal variability of air temperature in the occurrence of dengue in an area comprising seven municipalities of the greater são paulo.201729451659
estimating the size of aedes aegypti populations from dengue incidence data: implications for the risk of yellow fever outbreaks.in this paper we present a model to estimate the density of aedes mosquitoes in a community affected by dengue. the method consists in fitting a continuous function to the incidence of dengue infections, from which the density of infected mosquitoes is derived straightforwardly. further derivations allow the calculation of the latent and susceptible mosquitoes' densities, the sum of the three equals the total mosquitoes' density. the method is illustrated with the case of the risk of urban yello ...201730137722
evaluation of phytosynthesised silver nanoparticles from leaf extracts of leucas aspera and hyptis suaveolens and their larvicidal activity against malaria, dengue and filariasis vectors.the present study deals with the green synthesis of silver nanoparticle from the aqueous leaf extracts of leucas aspera and hyptis suaveolens as reducing agent and to investigate the larvicidal activity of synthesized silver nanoparticles. the synthesized silver nanoparticles were characterized by ultraviolet and visible absorption spectroscopy (uv), fourier transform-infrared spectroscopy (ft-ir), x-ray spectroscopy (xrd), field emission scanning electron microscope (fesem) and high-resonance t ...201729774292
biological control of anopheles darlingi, aedes aegypti and culex quinquefasciatus larvae using shrimps.mosquitoes can act as vectors of important diseases such as malaria, dengue, zika virus, yellow fever, chikungunya and mayaro fever, in addition to filariasis. the use of insecticides, larvicides, bed nets and repellents, besides the use of drugs as chemoprevention and the treatment of the sick are currently the pillars of the control of these vectors. we studied the biological control against of anopheles darlingi, aedes aegypti and culex quinquefasciatus larvae using shrimps of the species m. ...201729774286
ultrasound is not useful as a screening tool for dengue fever.dengue fever is a tropical disease that is transmitted by female aedes aegypti mosquitos. early diagnosis is necessary to reduce the mortality and morbidity associated with the disease. a combination of clinical, laboratory, and sonography findings can be potentially helpful in making an early diagnosis of dengue fever. there is paucity of literature on the use of ultrasound for dengue fever screening; hence, the primary objective of the study was to evaluate the utility of ultrasound as a scree ...201729657635
co-breeding association of aedes albopictus (skuse) and aedes aegypti (linnaeus) (diptera: culicidae) in relation to location and container size.the occurrence of major outbreaks of dengue, and other vector borne diseases such as chikungunya and zika in tropical and subtropical regions has rendered control of the diseases a top-priority for many affected countries including malaysia. control of the mosquito vectors aedes aegypti and aedes albopictus through the reduction of breeding sites and the application of insecticides to kill immature forms and adults are the main control efforts to combat these diseases. the present study describe ...201829644025
evaluation of a simple polytetrafluoroethylene (ptfe)-based membrane for blood-feeding of malaria and dengue fever vectors in the laboratory.controlled blood-feeding is essential for maintaining laboratory colonies of disease-transmitting mosquitoes and investigating pathogen transmission. we evaluated a low-cost artificial feeding (af) method, as an alternative to direct human feeding (dhf), commonly used in mosquito laboratories.201829642937
yellow fever.the yellow fever (yf) virus is a flavivirus, transmitted by haemagogus, sabethes or aedes aegypti mosquitoes. the disease is endemic in forest areas in africa and latin america leading to epizootics in monkeys that constitute the reservoir of the disease. there are two forms of yf: sylvatic, transmitted accidentally when approaching the forests, and urban, which can be perpetuated by aedes aegypti. in brazil, the last case of urban yf occurred in 1942. since then, there has been an expansion of ...201829641667
identification of entomopathogenic nematodes and symbiotic bacteria from nam nao national park in thailand and larvicidal activity of symbiotic bacteria against aedes aegypti and aedes albopictus.entomopathogenic nematodes (epns) that are symbiotically associated with xenorhabdus and photorhabdus bacteria can kill target insects via direct infection and toxin action. there are limited reports identifying such organisms in the national park of thailand. therefore, the objectives of this study were to identify epns and symbiotic bacteria from nam nao national park, phetchabun province, thailand and to evaluate the larvicidal activity of bacteria against aedes aegypti and ae. albopictus. a ...201829641570
suppression of the pelo protein by wolbachia and its effect on dengue virus in aedes aegypti.the endosymbiont wolbachia is known to block replication of several important arboviruses, including dengue virus (denv), in the mosquito vector aedes aegypti. so far, the exact mechanism of this viral inhibition is not fully understood. a recent study in drosophila melanogaster has demonstrated an interaction between the pelo gene and drosophila c virus. in this study, we explored the possible involvement of the pelo protein, that is involved in protein translation, in wolbachia-mediated antivi ...201829641562
exploring mosquito fauna of majuro atoll (republic of marshall islands) in the context of zika outbreak.first autochthonous zika clinical case was reported in the republic of marshall islands (rmi) on majuro atoll in february 2016. an entomological survey of mosquito larvae and adult populations was carried out in four areas of majuro, the most populated atoll of rmi encompassing different habitats (forest, rural, or urban) including some with confirmed clinical zika cases to evaluate which mosquito species could be involved in the zika transmission. a total of 2,367 immature and adult mosquito sp ...201829635382
a sex-structured model with birth pulse and release strategy for the spread of wolbachia in mosquito population.dengue fever is one of the most important diseases causing illness and death all over the world, which brings tremendous threat to peoples' life and property security, especially in the undeveloped areas. the main vector, aedes aegypti, must be controlled to prevent the transmission of dengue. there are a variety of methods to control it. wolbachia is an innovative bacterium which breaks the dengue transmission cycle for its characteristics of cytoplasmic incompatibility and maternal transmissio ...201829625205
efficacy of aedes aegypti control by indoor ultra low volume (ulv) insecticide spraying in iquitos, peru.aedes aegypti is a primary vector of dengue, chikungunya, zika, and urban yellow fever viruses. indoor, ultra low volume (ulv) space spraying with pyrethroid insecticides is the main approach used for ae. aegypti emergency control in many countries. given the widespread use of this method, the lack of large-scale experiments or detailed evaluations of municipal spray programs is problematic.201829624581
differences in the replicative capacities of clinical isolates of dengue virus in c6/36 cells and in urban populations of aedes aegypti from colombia, south america.dengue, the most prevalent arboviral disease worldwide, is caused by any of the four dengue virus (denv) serotypes that co-circulate constantly in hyperendemic areas such as medellin (colombia), and these serotypes are transmitted by mosquitoes of the genus aedes. in this study, we evaluated the replicative capacity of strains isolated in medellin between 2003 and 2007 in c6/36 cells and in colonies of aedes aegypti collected during 2010-2011 from high or low-incidence areas within the same city ...201830165044
direct nucleic acid analysis of mosquitoes for high fidelity species identification and detection of wolbachia using a cellphone.manipulation of natural mosquito populations using the endosymbiotic bacteria wolbachia is being investigated as a novel strategy to reduce the burden of mosquito-borne viruses. to evaluate the efficacy of these interventions, it will be critical to determine wolbachia infection frequencies in aedes aegypti mosquito populations. however, current diagnostic tools are not well-suited to fit this need. morphological methods cannot identify wolbachia, immunoassays often suffer from low sensitivity a ...201830161131
increased adult aedes aegypti and culex quinquefasciatus (diptera: culicidae) abundance in a dengue transmission hotspot, compared to a coldspot, within kaohsiung city, taiwan.the assumption that vector abundance differences might drive spatial and temporal heterogeneities in vector-borne disease transmission is common, though data supporting it is scarce. here, we present data from two common mosquito species aedes aegypti (linnaeus) and culex quinquefasciatus say, biweekly sampled as adults, from march 2016 through december 2017, with bg-sentinel traps in two neighboring districts of kaohsiung city (kc), taiwan. one district has historically been a dengue transmissi ...201830104501
impact of mosquito age and insecticide exposure on susceptibility of aedes albopictus (diptera: culicidae) to infection with zika virus.zika virus (zikv) is primarily transmitted to humans by aedes aegypti and ae. albopictus. vector⁻virus interactions influencing vector competence vary and depend on biological and environmental factors. a mosquito's chronological age may impact its immune response against virus infection. insecticides, source reduction, and/or public education are currently the best defense against mosquitoes that transmit zikv. this study assessed the effects of a mosquito's chronological age at time of infecti ...201830103526
regulation of midgut cell proliferation impacts aedes aegypti susceptibility to dengue virus.aedes aegypti is the vector of some of the most important vector-borne diseases like dengue, chikungunya, zika and yellow fever, affecting millions of people worldwide. the cellular processes that follow a blood meal in the mosquito midgut are directly associated with pathogen transmission. we studied the homeostatic response of the midgut against oxidative stress, as well as bacterial and dengue virus (denv) infections, focusing on the proliferative ability of the intestinal stem cells (isc). i ...201829782512
zika virus: report from the task force on tropical diseases by the world federation of societies of intensive and critical care medicine.zika fever is an acute infectious disease caused by the zika virus (zikv), an rna virus belonging to the genus flavivirus. zikv can be transmitted by bites from aedes aegypti and aedes albopictus species, sexual activity, blood transfusion and from mother to fetus during pregnancy and delivery. a total of 50 territories and countries in the americas had reported zikv infections. for those with symptoms, the presentation is similar to dengue fever with body aches, joint pain, fatigue, malaise, fe ...201829779827
genome sequence analysis of a novel bacillus thuringiensis strain blb406 active against aedes aegypti larvae, a novel potential bioinsecticide.blb406 is a novel isolate of bacillus thuringiensis with a larvicidal activity against aedes aegypti larvae. it displays original plasmidic and crystal protein patterns. the present work reported molecular and bioinformatic analyses for the genome sequence of blb406 using miseq illumina next-generation sequencing technology. the reads were assembled by velvet tool. using rast program and pgaap, the genome of blb406 strain was shown to contain 6297 genes corresponding to 5924 protein coding seque ...201829778876
chemical profile and pharmacological effects of the resin and essential oil from bursera slechtendalii: a medicinal "copal tree" of southern mexico.this study reports on the volatile profile and pharmacological activities of the essential oil and resin from bursera slechtendalii, an aromatic medicinal tree commercialized in southern mexico. the essential oil contained 25 volatiles including p-cymen-8-ol (25.4-35.7%), n-hexyl hexanoate (6.8-17.6%) and n-octyl hexanoate (7.6-14.8%), whereas the crystallized resin contained 14 volatiles, including n-octyl acetate (29.6-34.2%), limonene (15.8-18.7%) and n-octyl butyrate (12.6-17-6%). the inhala ...201829777752
modeling dengue vector population using remotely sensed data and machine learning.mosquitoes are vectors of many human diseases. in particular, aedes ægypti (linnaeus) is the main vector for chikungunya, dengue, and zika viruses in latin america and it represents a global threat. public health policies that aim at combating this vector require dependable and timely information, which is usually expensive to obtain with field campaigns. for this reason, several efforts have been done to use remote sensing due to its reduced cost. the present work includes the temporal modeling ...201829777650
use of bead-based serologic assay to evaluate chikungunya virus epidemic, haiti.the index case of chikungunya virus (chikv) in haiti was reported during early 2014; the vector, the pervasive aedes aegypti mosquito, promoted rapid spread throughout the country. during december 2014-february 2015, we collected blood samples from 4,438 persons at 154 sites (62 urban, 92 rural) throughout haiti and measured chikv igg by using a multiplex bead assay. overall chikv seroprevalence was 57.9%; differences between rural (mean 44.9%) and urban (mean 78.4%) areas were pronounced. logis ...201829774861
erratum to "comparative oviposition site selection in containers by aedes aegypti and aedes albopictus (diptera: culicidae) from florida". 201829618025
a comparative analysis of corpora allata-corpora cardiaca microrna repertoires revealed significant changes during mosquito metamorphosis.the corpora allata (ca) are a pair of endocrine glands with neural connections to the brain and close association with another neuroendocrine organ, the corpora cardiaca (cc). the ca from adult female aedes aegypti mosquitoes synthesize fluctuating levels of juvenile hormone (jh), which have been linked to the ovarian development and are influenced by nutritional signals. in this study, we investigated the potential involvement of micrornas (mirnas), a type of small non-coding rnas, in the regul ...201829605533
mosquito responses to trait- and density-mediated interactions of predation.mosquito and predatory larvae often share the same habitat. predators may influence mosquito prey populations through both lethal effect and non-lethal pathways. a series of experimental manipulations were used to distinguish between lethal (density-mediated interaction) and non-lethal (trait-mediated interaction) effects in a model system comprised of invasive prey mosquito, aedes aegypti, and a predatory mosquito toxorhynchites rutilus. treatments with predators present or manipulations mimick ...201829594613
effect of relaxation of deltamethrin pressure on metabolic resistance in a pyrethroid-resistant aedes aegypti (diptera: culicidae) strain harboring fixed p989p and g1016g kdr alleles.mutation of the voltage-gated sodium channel genes or knockdown resistance (kdr) and metabolic resistance in aedes aegypti (l.) (diptera: culicidae) are important resistance mechanisms against pyrethroids. the present study investigated the effect of relaxation of deltamethrin selection pressure on the level of mixed-function oxidases (mfo), when the allele frequency of s989p+v1016g mutations is fixed in a resistant ae. aegypti strain (upk-r) from chiang mai, thailand. the mosquitoes were divide ...201829590378
maternal invasion history of aedes aegypti and aedes albopictus into the isthmus of panama: implications for the control of emergent viral disease agents.despite an increase in dengue outbreaks and the arrival of chikungunya and zika disease in panama, studies on the demographic history of the invasive aedes mosquitoes that are the principle vectors of these diseases are still lacking in this region. here, we assess the genetic diversity of these mosquitoes in order to decipher their invasion histories into the isthmus of panama. dna sequences from the mitochondrial cytochrome c oxidase i gene obtained from 30 localities in 10 provinces confirmed ...201829579112
frequency and intensity of pyrethroid resistance through the cdc bottle bioassay and their association with the frequency of kdr mutations in aedes aegypti (diptera: culicidae) from mexico.the control of aedes aegypti (l.), the main urban vector that causes arboviral diseases such as dengue, chikungunya and zika, has proved to be a challenge because of a rapid increase in insecticide resistance. therefore, adequate monitoring of insecticide resistance is an essential element in the control of ae. aegypti and the diseases it transmits. we estimated the frequency and intensity (resistance frequency rapid diagnostic test [f-rdt] and resistance intensity rapid diagnostic test [i-rdt]) ...201829575404
population dynamics of engineered underdominance and killer-rescue gene drives in the control of disease vectors.a number of different genetics-based vector control methods have been proposed. two approaches currently under development in aedes aegypti mosquitoes are the two-locus engineered underdominance and killer-rescue gene drive systems. each of these is theoretically capable of increasing in frequency within a population, thus spreading associated desirable genetic traits. thus they have gained attention for their potential to aid in the fight against various mosquito-vectored diseases. in the case ...201829570717
repellent activity of trig (n-n diethyl benzamide) against man-biting mosquitoes.a study was conducted to assess efficacy of a new repellent brand trig (15% n-n diethyl benzamide) when compared to deet (20% n-n methyl toluamide). the repellents were tested in laboratory and field. in the laboratory, the repellence was tested on human volunteers, by exposing their repellent-treated arms on starved mosquitoes in cages for 3 minutes at hourly intervals, while counting the landing and probing attempts. anopheles gambiae and aedes aegypti mosquitoes were used. field evaluation wa ...201829552040
hr38, an ortholog of nr4a family nuclear receptors, mediates 20-hydroxyecdysone regulation of carbohydrate metabolism during mosquito reproduction.the aedes aegypti mosquito is the principal vector for many dangerous human viral diseases. carbohydrate metabolism (cm) is essential for supplying the energy necessary for host seeking, blood digestion and rapid egg development of this vector insect. the steroid hormone 20-hydroxyecdysone (20e) and the ecdysone receptor (ecr) are important regulators of cm, coordinating it with female reproductive events. we report here that the nr4a nuclear receptor ahr38 plays a critical role in mediating the ...201829526770
jh biosynthesis and hemolymph titers in adult male aedes aegypti mosquitoes.juvenile hormone (jh) is a major hormonal regulator in insects. in aedes aegypti females, jh signals the completion of the ecdysis to the adult stage and initiates reproductive processes. although the regulation of jh synthesis and titer in ae. aegypti females has been extensively studied, relatively little is known about changes of jh synthesis and titers in male mosquitoes, as well as on the roles of jh controlling male reproductive biology. a better understanding of male mosquito reproductive ...201829526769
mosquito pornoscopy: observation and interruption of aedes aegypti copulation to determine female polyandric event and mixed progeny.ades aegypti is the most important arbovirus vector in the world, and new strategies are under evaluation. biological studies mentioning the occurrence of a second mate in aedes aegypti can interfere with vector control program planning, which involves male mosquito release technique. this study presents different experiments to show the occurrence of mixed progeny. mixed male crosses (using a combination of different type of males in confinement with virgin females) showed no polyandric female. ...201829518112
comparative oviposition site selection in containers by aedes aegypti and aedes albopictus (diptera: culicidae) from florida.invasive container mosquito species aedes aegypti (l.) and ae. albopictus (skuse) frequently co-occur in nature where they are typically segregated spatially by distinctive macrohabitat preferences that may select for different oviposition strategies. in a standard cage environment, we compared oviposition site selection by individual gravid females of these species exposed to variable numbers of water-holding cups and the presence or absence of a rugose wettable container lining. offered plasti ...201829514300
building infestation index for aedes aegypti and occurrence of dengue fever in the municipality of foz do iguaçu, paraná, brazil, from 2001 to 2016.the building infestation index (bii) uses the rapid assay of the larval index for aedes aegypti (liraa) to express the relationship between positive and surveyed properties. we evaluated liraa and the relationship between the bii and climate variables for dengue cases in foz do iguaçu municipality, paraná.201829513847
occurrence of sweet refuse at disposal sites: rainwater retention capacity and potential breeding opportunities for aedes aegypti.nectar is the staple diet of adult mosquitoes in the wild, but its availability is inconsistent and can be affected by rainfall. in urban centers, aedes vectors commonly use man-made containers as their major habitat; however, they can colonize any items replenished by rainfall. garbage output has increased significantly in recent years, at a time when collection frequency is reducing. such garbage usually includes organic components, some of which are sweet and can be fed upon by other animals ...201829512008
the non-canonical notch signaling is essential for the control of fertility in aedes aegypti.the notch signaling pathway is a highly evolutionarily-conserved cell-cell signaling pathway that regulates many events during development. it plays a pivotal role in the regulation of fundamental cellular processes, such as cell proliferation, stem cell maintenance, and differentiation during embryonic and adult development. however, functions of notch signaling in aedes aegypti, the major mosquito vector for dengue, are largely unknown. in this study, we identified a unique feature of a. aegyp ...201829505577
dengue knowledge in indoor dengue patients from low socioeconomic class; aetiology, symptoms, mode of transmission and prevention.dengue fever has emerged as an emerging public health issue during last decade bearing significant morbidity and economic burden particularly in third world countries. current study aims to assess various domains of knowledge of indoor dengue patients..201829504327
resistance of aedes aegypti (diptera: culicidae) populations to deltamethrin, permethrin, and temephos in cambodia.dengue fever is a major public health concern, including 185,000 annual cases in cambodia. aedes aegypti is the primary vector for dengue transmission and is targeted with insecticide treatments. this study characterized the insecticide resistance status of ae aegypti from rural and urban locations. the susceptibility to temephos, permethrin, and deltamethrin of ae aegypti was evaluated in accordance with world health organization instructions. all the field populations showed lower mortality ra ...201829502428
bacterial microbiota of aedes aegypti mosquito larvae is altered by intoxication with bacillus thuringiensis israelensis.insect microbiota is a dynamic microbial community that can actively participate in defense against pathogens. bacillus thuringiensis (bt) is a natural entomopathogen widely used as a bioinsecticide for pest control. although bt's mode of action has been extensively studied, whether the presence of microbiota is mandatory for bt to effectively kill the insect is still under debate. an association between a higher tolerance and a modified microbiota was already evidenced but a critical point rema ...201829499735
anti-diuretic action of a capa neuropeptide against a subset of diuretic hormones in the disease vector aedes aegypti.the mosquito aedes aegypti is a vector responsible for transmitting various pathogens to humans, and their prominence as chief vectors of human disease is largely due to their anthropophilic blood feeding behaviour. larval stage mosquitoes must deal with the potential dilution of their haemolymph in freshwater, whereas the haematophagus a. aegypti female faces the challenge of excess ion and water intake after a blood meal. the excretory system, composed of the malpighian tubules (mts) and hindg ...201829496779
dengue in araraquara, state of são paulo: epidemiology, climate and aedes aegypti infestation.objective to describe the epidemiology of dengue in a medium-sized city in the state of são paulo. methods data, such as circulating serotypes, severe cases and deaths, age group, sex, among others, were obtained on reported and confirmed dengue cases in araraquara, state of são paulo, between 1991 and 2015. climatic and infestation data were also analyzed. these variables were evaluated descriptively, using statistical measures such as frequencies, averages, minimum and maximum. dengue incidenc ...201829489994
truck-mounted natular 2ec (spinosad) ulv residual treatment in a simulated urban environment to control aedes aegypti and aedes albopictus in north florida.preemptive treatment of dry habitats with an ultra-low volume (ulv) residual larvicide may be effective in an integrated vector management program to control populations of container-inhabiting aedes mosquitoes, key vectors of zika, dengue, and chikungunya viruses. we exposed dry, artificial containers placed in exposed and protected locations to natular 2ec (spinosad) larvicide applied with a truck-mounted ulv sprayer in a simulated urban setting in north florida, and later introduced water and ...201831442121
ultra-low volume application of spinosad (natular 2ec) larvicide as a residual in a tropical environment against aedes and anopheles species.we investigated the efficacy of a liquid larvicide, natular 2ec® (spinosad), applied with ultra-low volume sprayer as a residual application during the dry season in southeastern thailand against 4 medically important species-aedes aegypti, ae. albopictus, anopheles dirus, and an. minimus. we found that this larvicide could be applied as a residual to dry areas known to collect water and potentially still be effective after rains or irrigation, which could increase the flexibility and efficiency ...201831442120
effect of travel speed on dispersion of aqualuer 20-20 sprayed by a truck-mounted ultra-low-volume sprayer against caged aedes aegypti1.the effect of travel speed of a truck-mounted ultra-low-volume (ulv) sprayer on its application efficacy was studied at st. johns county fairground, elkton, fl, during summer 2015. the efficacy was assessed by spray deposition, droplet size spectrum, and 24-h mortality of caged adult aedes aegypti, using 2 rows of sampling locations, 15 m apart and spread up to 122 m from the spray. each location had a bioassay cage and an impinger droplet sampler, 1 m apart from each other, at 1.5 m off the gro ...201831442115
application efficacy of vectobac wdg against larval aedes aegypti using thermal fog technology.the effectiveness of thermal fog application of bacillus thuringiensis israelensis (vectobac® wdg) against larval aedes aegypti was evaluated in open and forest canopied environments in northeastern florida, using a handheld igeba® tf34 thermal fogger (with water conversion kit). the product was applied at the maximum label rate of 1 kg/ha to linear transects, in each environment, containing empty 0.5-liter cups. after application, late-2nd to early-3rd instars of ae. aegypti were added to each ...201831442110
ultra-low volume application of spinosad (natular 2ec) as a residual in a hot-arid environment against aedes aegypti.the invasive aedes aegypti is an important disease vector increasing in frequency in hot-arid regions of the usa such as the southwest. within hot-arid surroundings this mosquito may be confined to peridomestic locations that tend to be cooler and humid, such as in lush, irrigated ornamental vegetation surrounding homes. however, to reach these habitat refugia, ultra-low volume (ulv) applications of insecticides targeting this mosquito must retain efficacy after being sprayed from the air or str ...201831442108
mosquitocidal activity of a naturally occurring isochroman and synthetic analogs from the plant pathogenic fungus, diaporthe eres against aedes aegypti (diptera: culicidae).the culture filtrate of a plant pathogenic fungus that infects english ivy (hedera helix l., araliaceae) was investigated for mosquitocidal constituents by bioassay-guided isolation. the fungus responsible for pathogenic effects on the plant h. helix has been identified as diaporthe eres nitschke by molecular techniques. the mosquito adulticidal constituent in the culture filtrate was identified as 3,4-dihydro-8-hydroxy-3,5-dimethylisocoumarin (1) by spectroscopic techniques. laboratory bioassay ...201829474636
comparative analysis of major mosquito vectors response to seed-derived essential oil and seed pod-derived extract from acacia nilotica.botanical metabolites are increasingly realized as potential replacements to chemical insecticides. in the present study, acacia nilotica seed essential oil and seed pod solvent extracts were tested for bioefficacy against three important types of mosquitoes. mortality was recorded 24 h post-treatment, while smoke toxicity of adult mosquitoes was recorded at 10 min intervals for 40 min. seed pod powder was extracted with different solvents and hydrodistilled seed oil chemical constituents were d ...201829473901
socioeconomic and human behavioral factors associated with aedes aegypti (diptera: culicidae) immature habitat in tucson, az.aedes aegypti (l.; diptera: culicidae) has been established in the southwestern united states for several decades, but relationships between humans and mosquitoes in this arid region are not well-characterized. in august 2012, the outdoor premises of 355 houses within 20 neighborhoods in tucson, arizona were surveyed for containers that could provide larval habitat for ae. aegypti mosquitoes. at the same time, a knowledge, attitudes and practices (kap) questionnaire was administered to a residen ...201829471405
salinity tolerant aedes aegypti and ae. albopictus-infection with dengue virus and contribution to dengue transmission in a coastal peninsula.aedes aegypti and ae. albopictus are major arboviral vectors that are considered to lay eggs, and undergo preimaginal development only in fresh water collections. however, recently they have been also shown to develop in coastal brackish water habitats. the ability of the biologically variant salinity-tolerant aedes vectors to transmit arboviral diseases is unknown. we therefore, investigated the infection of salinity-tolerant aedes mosquitoes with dengue virus (denv) and analysed dengue inciden ...201829916445
screening of natural wolbachia infection in aedes aegypti, aedes taeniorhynchus and culex quinquefasciatus from guadeloupe (french west indies).guadeloupe islands are threatened by several mosquito-borne viruses such as dengue, chikungunya, zika and west nile virus. it appears essential to look for alternative mosquito control methods such as the incompatible insect technique (itt) aiming at sterilizing wild females by inundative releases of incompatible males. before considering the implementation of such a strategy, the characterization of genetic diversity of the endocellular bacterium wolbachia regarding the local mosquito populatio ...201829908171
prediction and prevention of urban arbovirus epidemics: a challenge for the global virology community.the recent emergence and rapid spread of zika virus in tropical regions of the western hemisphere took arbovirologists and public health officials by surprise, and the earlier transfers of west nile and chikungunya viruses from the old to the new world were also unexpected. these pandemics underscore the increasing threat of zoonotic arboviruses, especially those that are capable of entering into human-amplified, urban transmission cycles transmitted by aedes (stegomyia) aegypti and sometimes ot ...201829906475
lack of evidence for natural wolbachia infections in aedes aegypti (diptera: culicidae).wolbachia is a genus of endosymbiotic bacteria that infects 66% of all insect species. its major impact on insects is in reproduction: sterility, production of one sex, and/or parthenogenesis. another effect was discovered when the disease-transmitting mosquito, aedes aegypti linnaeus (diptera: culicidae), was infected with wolbachia isolated from drosophila: infected female mosquitoes became less capable of transmitting diseases such as dengue fever and chikungunya. this has led to releases of ...201829901734
restoration of pyrethroid susceptibility in a highly resistant aedes aegypti population.insecticide resistance has evolved in disease vectors worldwide, creating the urgent need to either develop new control methods or restore insecticide susceptibility to regain use of existing tools. here we show that phenotypic susceptibility can be restored in a highly resistant field-derived strain of aedes aegypti in only 10 generations through rearing them in the absence of insecticide.201829899128
signifying zika: heterogeneity in the representations of the virus by history of infection.despite having been broadly advertised by the mass media, many negative consequences of the zika virus have been less significant than originally predicted. it is likely that after a few months from the epidemic's onset, personal experience with the virus has altered the person's way to deal with the disease. this study explores the relation between exposure to zika virus and the social representation of the epidemic. more specifically, one analyzes if increased exposure to the risk of zika infe ...201829898004
evaluation of the effects of the larvicides temephos on reproductive performance, embryofetal development and dna integrity of swiss mice.temephos is considered the gold standard by the ministry of health for controlling the larvae of the mosquito aedes aegypti. the present study evaluated the effects of temephos larvicide on the reproductive performance, embryo-fetal development and dna integrity of swiss mice. this study used 30 pregnant female mice: 10 were controls treated with drinking water at a dosage of 0.1 ml/10 g (body weight - b.w., administered orally - a.o.), and 20 were treated with temephos at doses of 0.0043 mg/kg ...201829891373
what does the future hold for yellow fever virus? (i).the recent resurgence of yellow fever virus (yfv) activity in the tropical regions of africa and south america has sparked renewed interest in this infamous arboviral disease. yellow fever virus had been a human plague for centuries prior to the identification of its urban transmission vector, the aedes (stegomyia) aegypti (linnaeus) mosquito species, and the development of an efficient live-attenuated vaccine, the yf-17d strain. the combination of vector-control measures and vaccination campaig ...201829890711
a transcriptomic survey of the impact of environmental stress on response to dengue virus in the mosquito, aedes aegypti.populations of aedes aegypti naturally exhibit variable susceptibility to dengue viruses. this natural variation can be impacted by nutritional stress resulting from larval-stage crowding, indicating the influence of environment components on the adult mosquito immune response. in particular, larval crowding was previously shown to reduce the susceptibility of adult females of a trinidad field isolate of a. aegypti to the dengue serotype 2 (jam1409) virus. here, we present the first whole transc ...201829889847
target and non-target toxicity of fern extracts against mosquito vectors and beneficial aquatic organisms.dengue and malaria are significant mosquito-borne diseases that are rapidly spread worldwide, mainly in temperate countries. pteridophytes were identified to be a significant source of novel mosquitocidal agents. the present research was to explore the eco-friendly larvicides from methanol extracts of ferns, viz., actiniopteris radiata, adiantum caudatum, cheilanthes swartzii, hemionitis arifolia and lycopodium clavatum. the larvicidal potential of the extracts screened using larvae of dengue ve ...201829885618
viral determinants and vector competence of zika virus transmission.zika virus (zikv) has emerged as a new global health threat. since its first discovery in zika forest in uganda, this virus has been isolated from several mosquito species, including aedes aegypti and aedes albopictus. the geographical distribution of these mosquito species across tropical and subtropical regions has led to several outbreaks, including the recent pandemic in brazil, followed by the pacific islands and other areas of north and south america. this has gained attention of the scien ...201829875751
multiscale approach to deciphering the molecular mechanisms involved in the direct and intergenerational effect of ibuprofen on mosquito aedes aegypti.the anti-inflammatory ibuprofen is a ubiquitous surface water contaminant. however, the chronic impact of this pharmaceutical on aquatic invertebrate populations remains poorly understood. in model insect aedes aegypti, we investigated the intergenerational consequences of parental chronic exposure to an environmentally relevant concentration of ibuprofen. while exposed individuals did not show any phenotypic changes, their progeny showed accelerated development and an increased tolerance to sta ...201829874051
effects of temephos, permethrin, and eucalyptus nitens essential oil on survival and swimming behavior of aedes aegypti and anopheles pseudopunctipennis (diptera: culicidae) larvae.an essential strategy to deal with mosquito-borne diseases is the control of larvae in their development sites. the mosquitoes anopheles pseudopunctipennis (theobald) (diptera: culicidae), a malaria vector, and aedes aegypti (l.) (diptera: culicidae), vector of dengue, zika, yellow fever, and chikungunya viruses, breed in very different habitats. insecticide treatments of mosquito larvae focus mainly on their lethal effects. however, insecticide degradation or the poor dosage of larvicides will ...201829873741
vertical transmission of dengue virus in the yogyakarta airport area.international health regulations controls international travel including human movement, disease vector, and imported items to prevent the spread of dengue, especially in seaports, airports, and border crossing posts. this study aimed to determine dengue transovarial transmission index (tti) and distribution of dengue virus in the areas around adisucipto airport of yogyakarta, indonesia.201829871615
differential effects of temperature and mosquito genetics determine transmissibility of arboviruses by aedes aegypti in argentina.aedes aegypti (l.) (diptera: culicidae) have a global distribution and are the primary vector of a number of mosquito-borne viruses responsible for epidemics throughout the americas. as in much of south america, the threat from pathogens including dengue virus (denv; flaviviridae, flavivirus) and chikungunya virus (chikv; togaviridae, alphavirus) has increased in argentina in recent years. the complexity of transmission cycles makes predicting the occurrence and intensity of arbovirus outbreaks ...201829869610
serosurvey of human antibodies recognizing aedes aegypti d7 salivary proteins in colombia.dengue is one of the most geographically significant mosquito-borne viral diseases transmitted by aedes mosquitoes. during blood feeding, mosquitoes deposit salivary proteins that induce antibody responses. these can be related to the intensity of exposure to bites. some mosquito salivary proteins, such as d7 proteins, are known as potent allergens. the antibody response to d7 proteins can be used as a marker to evaluate the risk of exposure and disease transmission and provide critical informat ...201829868532
effect of the topical repellent para-menthane-3,8-diol on blood feeding behavior and fecundity of the dengue virus vector aedes aegypti.dengue fever is an acute disease caused by the dengue virus and transmitted primarily by the mosquito aedes aegypti. the current strategy for dengue prevention is vector control including the use of topical repellents to reduce mosquito biting. although n,n-diethyl-m-methylbenzamide (deet) is the most common active ingredient in topical repellent products, para-menthane-3,8-diol (pmd) is also used commercially. studies have indicated pmd reduced biting by 90⁻95% for up to 6⁻8 h, similar to the e ...201829867036
pyrethroid resistance in the dengue vector aedes aegypti in southeast asia: present situation and prospects for management.human arboviral diseases transmitted by aedes aegypti such as dengue, zika, chikungunya and yellow fever remain global public health threats to date. of these diseases, dengue fever is particularly prevalent in southeast asia. relentless vector control efforts are performed to curtail disease transmissions through which pyrethroid insecticides are broadly used as the first line of defense to control ae. aegypti, especially in the course of disease outbreaks. here, we compile the largest contempo ...201829866193
molecular and functional characterization of single-box high-mobility group b (hmgb) chromosomal protein from aedes aegypti.high-mobility group b (hmgb) proteins have highly conserved, unique dna-binding domains, hmg boxes, that can bind non-b-type dna structures, such as bent, kinked and unwound structures, with high affinity. hmgb proteins also promote dna bending, looping and unwinding. in this study, we determined the role of the aedes aegypti single hmg-box domain protein aahmgb; characterized its structure, spatiotemporal expression levels, subcellular localization, and nucleic acid binding activities; and comp ...201829859286
technologies to combat aedes mosquitoes: a model based on smart city.aedes aegypti and aedes albopictus mosquitoes are responsible for the transmission of diseases such as dengue fever, yellow fever, chikungunya fever, zika virus fever, some of which can cause irreversible central nervous system problems and death. this study investigates what technologies are being used for combatting and monitoring the aedes mosquitoes and to propose joining these technologies into a single and complete solution using the smart cities concept. a search for newscasts on google a ...201829857404
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