Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| an aedes aegypti-associated fungus increases susceptibility to dengue virus by modulating gut trypsin activity. | transmission of dengue virus (denv) requires successful completion of the infection cycle in the aedes aegypti vector, which is initiated in the midgut tissue after ingestion of an infectious blood meal. while certain ae. aegypti midgut-associated bacteria influence virus infection, little is known about the midgut-associated fungi (mycobiota), and how its members might influence susceptibility to denv infection. we show that a talaromyces (tsp_pr) fungus, isolated from field-caught ae. aegypti, ... | 2017 | 29205153 |
| alternative patterns of sex chromosome differentiation in aedes aegypti (l). | some populations of west african aedes aegypti, the dengue and zika vector, are reproductively incompatible; our earlier study showed that divergence and rearrangements of genes on chromosome 1, which bears the sex locus (m), may be involved. we also previously described a proposed cryptic subspecies senaae (pk10, senegal) that had many more high inter-sex fst genes on chromosome 1 than did ae.aegypti aegypti (aaa, pai lom, thailand). the current work more thoroughly explores the significance of ... | 2017 | 29202694 |
| global transcriptome analysis of aedes aegypti mosquitoes in response to zika virus infection. | zika virus (zikv) of the flaviviridae family is a recently emerged mosquito-borne virus that has been implicated in the surge of the number of microcephaly instances in south america. the recent rapid spread of the virus led to its declaration as a global health emergency by the world health organization. the virus is transmitted mainly by the mosquito aedes aegypti, which is also the vector of dengue virus; however, little is known about the interactions of the virus with the mosquito vector. i ... | 2017 | 29202041 |
| global risk mapping for major diseases transmitted by aedes aegypti and aedes albopictus. | the objective of this study was to map the global risk of the major arboviral diseases transmitted by aedes aegypti and aedes albopictus by identifying areas where the diseases are reported, either through active transmission or travel-related outbreaks, as well as areas where the diseases are not currently reported but are nonetheless suitable for the vector. | 2017 | 29196275 |
| larvicidal and residual activity of imidazolium salts against aedes aegypti (diptera: culicidae). | aedes aegypti is an important species of mosquito, which can transmit several arboviruses such as dengue fever, yellow fever, chikungunya, and zika. as these mosquitoes are becoming resistant to most chemical insecticides used in the world, studies with new larvicides should be prioritized. based on the known biological profile of imidazolium salts (is), the objective of this study was to evaluate the potential of six is as larvicides against ae. aegypti, which was tested against ae. aegypti lar ... | 2017 | 29193680 |
| first insights on insecticidal activity upon aedes aegypti and partial biochemical characterization of a novel low molecular mass chymotrypsin-trypsin inhibitor purified from lonchocarpus sericeus seeds. | arboviroses such as dengue, zika and chikungunya represent a serious issue in public health due to the absence of approved vaccine or specific antiviral drugs. one way to prevent these diseases is by combating the vector mosquito, aedes aegypti (diptera), which has serine proteases in the midgut. protease inhibitors are molecules that can block enzyme activity impairing digestion and nutrition that can lead to death. thus, we purified and characterized a novel chymotrypsin- trypsin inhibitor fro ... | 2017 | 29193604 |
| chikungunya: molecular aspects, clinical outcomes and pathogenesis. | the alarming worldwide emergence of the chikungunya virus began in the last decade. since the first autochthonous transmission in mexico in november of 2014, the virus has spread throughout the country, resulted in multiple outbreaks. this virus produces an acute and self-limiting disease characterized by fever, polyarthralgia, myalgia, exanthema, and general malaise. it is transmitted to humans by the bite of aedes aegypti and a. albopictus mosquitoes. the fact that the clinical presentation is ... | 2017 | 29265115 |
| characteristics of aedes aegypti adult mosquitoes in rural and urban areas of western and coastal kenya. | aedes aegypti is the main vector for yellow fever, dengue, chikungunya and zika viruses. recent outbreaks of dengue and chikungunya have been reported in kenya. presence and abundance of this vector is associated with the risk for the occurrence and transmission of these diseases. this study aimed to characterize the presence and abundance of ae. aegypti adult mosquitoes from rural and urban sites in western and coastal regions of kenya. presence and abundance of ae. aegypti adult mosquitoes wer ... | 2017 | 29261766 |
| individual co-variation between viral rna load and gene expression reveals novel host factors during early dengue virus infection of the aedes aegypti midgut. | dengue virus (denv) causes more human infections than any other mosquito-borne virus. the current lack of antiviral strategies has prompted genome-wide screens for host genes that are required for denv infectivity. earlier transcriptomic studies that identified denv host factors in the primary vector aedes aegypti used inbred laboratory colonies and/or pools of mosquitoes that erase individual variation. here, we performed transcriptome sequencing on individual midguts in a field-derived ae. aeg ... | 2017 | 29261661 |
| assessment of maldi-tof mass spectrometry for filariae detection in aedes aegypti mosquitoes. | matrix assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof ms) is an emerging tool for routine identification of bacteria, archaea and fungi. it has also been recently applied as an accurate approach for arthropod identification. preliminary studies have shown that the maldi-tof ms was able to differentiate whether ticks and mosquitoes were infected or not with some bacteria and plasmodium parasites, respectively. the aim of the present study was to test the efficien ... | 2017 | 29261659 |
| origin of a high-latitude population of aedes aegypti in washington, dc. | an overwintering population of aedes aegypti has been documented in the capitol hill neighborhood of washington, dc, since 2011. mitochondrial cytochrome oxidase i (mtcoi) sequence data presented in a previous study traced the origin to the new world. here, we use microsatellite and snp chip data along with more mitochondrial dna (mtdna) sequences on washington ae. aegypti samples and samples from potential sources to further narrow the origin of this population. genetically, washington ae. aegy ... | 2017 | 29260658 |
| from ground pools to treeholes: convergent evolution of habitat and phenotype in aedes mosquitoes. | invasive mosquito species are responsible for millions of vector-borne disease cases annually. the global invasive success of aedes mosquitoes such as aedes aegypti and aedes albopictus has relied on the human transport of immature stages in container habitats. however, despite the importance of these mosquitoes and this ecological specialization to their widespread dispersal, evolution of habitat specialization in this group has remained largely unstudied. we use comparative methods to evaluate ... | 2017 | 29258425 |
| zika virus exhibits lineage-specific phenotypes in cell culture, in aedes aegypti mosquitoes, and in an embryo model. | zika virus (zikv) has quietly circulated in africa and southeast asia for the past 65 years. however, the recent zikv epidemic in the americas propelled this mosquito-borne virus to the forefront of flavivirus research. based on historical evidence, zikv infections in africa were sporadic and caused mild symptoms such as fever, skin rash, and general malaise. in contrast, recent asian-lineage zikv infections in the pacific islands and the americas are linked to birth defects and neurological dis ... | 2017 | 29258204 |
| seasonal and spatial quantitative changes in aedes aegypti under distinctly different ecological areas of lahore, pakistan. | to find out the variations in larval and adult density of aedes aegypti in different seasons under different ecological conditions. | 2017 | 29256519 |
| social-ecological factors and preventive actions decrease the risk of dengue infection at the household-level: results from a prospective dengue surveillance study in machala, ecuador. | in ecuador, dengue virus (denv) infections transmitted by the aedes aegypti mosquito are among the greatest public health concerns in urban coastal communities. community- and household-level vector control is the principal means of controlling disease outbreaks. this study aimed to assess the impact of knowledge, attitudes, and practices (kaps) and social-ecological factors on the presence or absence of denv infections in the household. | 2017 | 29253873 |
| an approach of the exact linearization techniques to analysis of population dynamics of the mosquito aedes aegypti. | dengue fever, chikungunya and zika are caused by different viruses and mainly transmitted by aedes aegypti mosquitoes. these diseases have received special attention of public health officials due to the large number of infected people in tropical and subtropical countries and the possible sequels that those diseases can cause. in severe cases, the infection can have devastating effects, affecting the central nervous system, muscles, brain and respiratory system, often resulting in death. vaccin ... | 2017 | 29253493 |
| aedes aegypti resistance development to commonly used insecticides in jakarta, indonesia. | aedes aegypti is the primary vector of various relevant arthropod-borne viral infectious diseases worldwide. the mosquito control is still mainly performed by using insecticides but their effectiveness is increasingly questioned nowadays. we here conducted a study on ae. aegypti resistance development towards several commonly used insecticides in the capital city of jakarta, indonesia. in order to achieve this goal, ae. aegypti eggs from jakarta were collected with ovitraps and hatched in the in ... | 2017 | 29253003 |
| aedes aegypti microrna mir-2b regulates ubiquitin-related modifier to control chikungunya virus replication. | arboviruses that replicate in mosquitoes activate innate immune response within mosquitoes. regulatory non-coding micrornas (mirna) are known to be modulated in mosquitoes during chikungunya infection. however, information about targets of these mirnas is scant. the present study was aimed to identify and analyze targets of mirnas that are regulated during chikungunya virus (chikv) replication in aedes aegypti cells and in the mosquito. employing next-generation sequencing technologies, we ident ... | 2017 | 29247247 |
| zika virus can be venereally transmitted between aedes aegypti mosquitoes. | alternative transmission routes have been described for zika virus (zikv). here, we assessed for the first time the venereal transmission of zikv between aedes aegypti under laboratory conditions. | 2017 | 29246237 |
| insecticide susceptibility screening against culex and aedes (diptera: culicidae) mosquitoes from the united states. | mosquitoes exposed to sublethal doses of insecticides may be selected for resistance to insecticide active ingredients (ais). mosquitoes are exposed to ais through agricultural, public/private mosquito control programs, homeowners, and other sources. hence, mosquito control programs should routinely measure the resistance/susceptibility status of mosquito populations of public health concern. the objectives here were to determine resistance status for six ais used in adult mosquito control in th ... | 2017 | 29186579 |
| involvement of cecropin b in the formation of the aedes aegypti mosquito cuticle. | in this study, we found a mosquito antimicrobial peptide (amp), aedes aegypti cecropin b (aacec b), was expressed constitutively in pupae. knockdown in the pupae of aacec b using double-stranded rna (dsrna) resulted in high mortality, the emergence of deformed adults and an impairment of pharate adult cuticle formation with fewer lamellae being deposited and the helicoidal pattern of the chitin microfibrils being disorganized. simultaneous injection of aacec b dsrna and aacec b peptide into pupa ... | 2017 | 29180688 |
| a peptide signaling system that rapidly enforces paternity in the aedes aegypti mosquito. | female aedes aegypti mosquitoes typically mate only once with one male in their lifetime, a behavior known as "monandry" [1]. this single mating event provisions the female with sufficient sperm to fertilize the >500 eggs she will produce during her ∼4- to 6-week lifespan in the laboratory [2]. successful mating induces lifetime refractoriness to subsequent insemination by other males, enforcing the paternity of the first male [3-5]. ae. aegypti mate in flight near human hosts [6], and females b ... | 2017 | 29174895 |
| emergent acoustic order in arrays of mosquitoes. | the yellow fever mosquito aedes aegypti forms aerial swarms that serve as mating aggregations [1]. despite lacking the remarkable collective order of other animal ensembles, such as fish and birds [2], the kinematic properties of these swarms bear the hallmarks of local interaction and global cohesion [3,4]. however, the mechanisms responsible for collective behaviour in mosquitoes are not well understood. mosquitoes use their antennae as hearing organs to locate and interact with one another vi ... | 2017 | 29161555 |
| dengue virus detection in aedes aegypti larvae and pupae collected in rural areas of anapoima, cundinamarca, colombia. | there is a high incidence and prevalence of dengue in the department of cundinamarca, and recently aedes aegypti, the main vector of dengue virus (denv), was detected in some of its rural areas. | 2017 | 29161491 |
| effect of the type and number of washes in the efficacy of long lasting insecticidal nets for aedes aegypti control in girardot, colombia. | the use of long lasting insecticidal materials has shown promising results in the control of aedes aegypti. | 2017 | 29161489 |
| virological surveillance of aedes (stegomyia) aegypti and aedes (stegomyia) albopictus as support for decision making for dengue control in medellín. | dengue represents an important public health problem in colombia. no treatment is available and the vaccine has not been approved in all countries, hence, actions should be strengthened to mitigate its impact through the control of aedes aegypti, the vector mosquito. in colombia, surveillance is done using entomological indexes and case notification, which is usually informed late, leading to untimely interventions. viral detection in urban mosquitoes using molecular techniques provides more acc ... | 2017 | 29161487 |
| aedes albopictus (skuse, 1894) infected with the american-asian genotype of dengue type 2 virus in medellín suggests its possible role as vector of dengue fever in colombia. | aedes aegypti and ae. albopictus are recognized vectors of dengue, yellow fever, chikungunya and zika arboviruses in several countries worldwide. in colombia, ae. albopictus geographical distribution has increased to include highly populated cities such as cali and medellín. although this species has been frequently found in urban and semi-urban zones in the country, its role as vector of the dengue fever is poorly known. | 2017 | 29161485 |
| [laboratory susceptibility tests of aedes aegypti and culex quinquefasciatus larvae to the entomopathogenic nematode heterorhabditis bacteriophora]. | aedes aegypti is the vector of dengue, yellow fever, zika and chikungunya viruses, and culex quinquefasciatus is the vector of st. louis and west nile encephalitis viruses. | 2017 | 29161479 |
| [spatial distribution of potential and positive aedes aegypti breeding sites]. | the spatial distribution of aedes aegypti is heterogeneous, and the interaction between positive and potential breeding sites located both inside and outside homes is one of the most difficult aspects to characterize in vector control programs. | 2017 | 29161478 |
| [spatial distribution of aedes aegypti (diptera: culicidae) in the rural area of two municipalities of cundinamarca, colombia]. | it is difficult to adapt or create new strategies for dengue control in rural areas due to the dispersion of homes and the lack of knowledge about the presence and behavior of the vector in these areas. spatial data analysis could help understand time and space dynamics related to the transmission of vector-borne diseases in rural areas and, thus, contribute to the prevention and control of dengue. | 2017 | 29161476 |
| alternative control of aedes aegypti resistant to pyrethroids: lethal and sublethal effects of monoterpenes bioinsecticides. | the mosquito aedes aegypti is intensely controlled because it is vector of viruses that cause innumerous diseases, especially in tropical regions. due to the indiscriminate use of insecticides, populations from different regions have been resistant to pyrethroids. here, we analyzed the lethal and sublethal effects of essential oil of aristolochia trilobata and its major compounds on a. aegypti from susceptible and pyrethroid resistant populations. | 2017 | 29160036 |
| upregulation of aedes aegypti vago1 by wolbachia and its effect on dengue virus replication. | dengue infection along with its related disease conditions poses a significant threat to human health. the pathogen responsible for this infection is dengue virus (denv) which is primarily transmitted to humans through the bites of aedes aegypti mosquitoes. unavailability of a potent vaccine has recently sparked renewed research endeavours aimed at vector control. to date, wolbachia as an endosymbiotic bacterium has shown promise as a novel biocontrol agent to restrict denv replication in the ve ... | 2017 | 29157676 |
| a call to introduce structured zika surveillance in india. | india has the climatic conditions conducive to year-round transmission of zika virus, and a structured disease surveillance program should be implemented to prevent an outbreak. such a program should (i) start screening before an outbreak arises; (ii) collect baseline data to assess future disease risk and monitor potential birth defects; and (iii) provide new insights into the ecology of the disease and inform public health policy following the one health concept. | 2017 | 29153262 |
| effective population sizes of a major vector of human diseases, aedes aegypti. | the effective population size (ne ) is a fundamental parameter in population genetics that determines the relative strength of selection and random genetic drift, the effect of migration, levels of inbreeding, and linkage disequilibrium. in many cases where it has been estimated in animals, ne is on the order of 10%-20% of the census size. in this study, we use 12 microsatellite markers and 14,888 single nucleotide polymorphisms (snps) to empirically estimate ne in aedes aegypti, the major vecto ... | 2017 | 29151858 |
| vectors, hosts, and control measures for zika virus in the americas. | we examine zika virus (zikv) from an ecological perspective and with a focus on the americas. we assess (1) the role of wildlife in zikv disease ecology, (2) how mosquito behavior and biology influence disease dynamics, and (3) how nontarget species and ecosystems may be impacted by vector control programs. our review suggests that free-ranging, non-human primates may be involved in zikv transmission in the old world; however, other wildlife species likely play a limited role in maintaining or t ... | 2017 | 29150828 |
| the introduction of dengue follows transportation infrastructure changes in the state of acre, brazil: a network-based analysis. | human mobility, presence and passive transportation of aedes aegypti mosquito, and environmental characteristics are a group of factors which contribute to the success of dengue spread and establishment. to understand this process, we assess data from dengue national and municipal basins regarding population and demographics, transportation network, human mobility, and ae. aegypti monitoring for the brazilian state of acre since the first recorded dengue case in the year 2000 to the year 2015. d ... | 2017 | 29149175 |
| baccharis reticularia dc. and limonene nanoemulsions: promising larvicidal agents for aedes aegypti (diptera: culicidae) control. | baccharis reticularia dc. is a plant species from the asteraceae family that is endemic to brazil. despite the great importance of baccharis genus, no study has been carried out regarding either the phytochemical composition of b. reticularia or the evaluation of its larvicidal potential. considering the intrinsic immiscibility of essential oils, this study shows larvicidal nanoemulsions containing the b. reticularia phytochemically characterized essential oil and its main constituent against ae ... | 2017 | 29149027 |
| [dengue fever : symptoms, epidemiology, entomology, pathogen diagnosis and prevention]. | dengue infections are among the most frequent causes of febrile disease in tropical climates. infections are caused by a flavivirus transmitted by the aedes mosquito. aedes aegypti mosquitos are the main transmitters of dengue viruses. since these insects are both diurnal and container breeders, particular prevention and control measures are required. symptom severity varies and can range from a mild, flu-like clinical picture to severe hemorrhage and shock. the most common symptoms experienced ... | 2017 | 29147722 |
| development and physiological effects of an artificial diet for wolbachia-infected aedes aegypti. | the endosymbiotic bacterium wolbachia spreads rapidly through populations of aedes aegypti mosquitoes, and strongly inhibits infection with key human pathogens including the dengue and zika viruses. mosquito control programs aimed at limiting transmission of these viruses are ongoing in multiple countries, yet there is a dearth of mass rearing infrastructure specific to wolbachia-infected mosquitoes. one example is the lack of a blood meal substitute, which accounts for the wolbachia-specific ph ... | 2017 | 29146940 |
| behavioral, climatic, and environmental risk factors for zika and chikungunya virus infections in rio de janeiro, brazil, 2015-16. | the burden of arboviruses in the americas is high and may result in long-term sequelae with infants disabled by zika virus infection (zikv) and arthritis caused by infection with chikungunya virus (chikv). we aimed to identify environmental drivers of arbovirus epidemics to predict where the next epidemics will occur and prioritize municipalities for vector control and eventual vaccination. we screened sera and urine samples (n = 10,459) from residents of 48 municipalities in the state of rio de ... | 2017 | 29145452 |
| zika virus: an updated review of competent or naturally infected mosquitoes. | zika virus (zikv) is an arthropod-borne virus (arbovirus) that recently caused outbreaks in the americas. over the past 60 years, this virus has been observed circulating among african, asian, and pacific island populations, but little attention has been paid by the scientific community until the discovery that large-scale urban zikv outbreaks were associated with neurological complications such as microcephaly and several other neurological malformations in fetuses and newborns. this paper is a ... | 2017 | 29145400 |
| shifting the paradigm in dirofilaria immitis prevention: blocking transmission from mosquitoes to dogs using repellents/insecticides and macrocyclic lactone prevention as part of a multimodal approach. | this study assessed the influence of a topical ectoparasiticide (dinotefuran-permethrin-pyriproxyfen, dpp, vectra® 3d, ceva animal health) combined with a macrocyclic lactone (milbemycin oxime, mbo, interceptor®, virbac) on transmission of heartworm l3 from mosquitoes to dogs and subsequent development of worms in treated dogs exposed to infected mosquitoes. | 2017 | 29143678 |
| blocking the transmission of heartworm (dirofilaria immitis) to mosquitoes (aedes aegypti) by weekly exposure for one month to microfilaremic dogs treated once topically with dinotefuran-permethrin-pyriproxyfen. | this study assessed the influence of a topical ectoparasiticide (dinotefuran-permethrin-pyriproxyfen, dpp, vectra®3d, ceva animal health) on the acquisition of heartworm microfilariae by mosquitoes exposed to microfilaremic dogs weekly for 1 month. | 2017 | 29143651 |
| corrigendum: rna-seq comparison of larval and adult malpighian tubules of the yellow fever mosquito aedes aegypti reveals life stage-specific changes in renal function. | [this corrects the article on p. 283 in vol. 8, pmid: 28536536.]. | 2017 | 29142533 |
| modelling dengue fever risk in the state of yucatan, mexico using regional-scale satellite-derived sea surface temperature. | accurately predicting vector-borne diseases, such as dengue fever, is essential for communities worldwide. changes in environmental parameters such as precipitation, air temperature, and humidity are known to influence dengue fever dynamics. furthermore, previous studies have shown how oceanographic variables, such as el niño southern oscillation (enso)-related sea surface temperature from the pacific ocean, influences dengue fever in the americas. however, literature is lacking on the use of re ... | 2017 | 28450208 |
| detecting the impact of temperature on transmission of zika, dengue, and chikungunya using mechanistic models. | recent epidemics of zika, dengue, and chikungunya have heightened the need to understand the seasonal and geographic range of transmission by aedes aegypti and ae. albopictus mosquitoes. we use mechanistic transmission models to derive predictions for how the probability and magnitude of transmission for zika, chikungunya, and dengue change with mean temperature, and we show that these predictions are well matched by human case data. across all three viruses, models and human case data both show ... | 2017 | 28448507 |
| juvenile hormone and its receptor methoprene-tolerant promote ribosomal biogenesis and vitellogenesis in the aedes aegypti mosquito. | juvenile hormone (jh) controls many biological activities in insects, including development, metamorphosis, and reproduction. in the aedes aegypti mosquito, a vector of dengue, yellow fever, chikungunya, and zika viruses, the metabolic tissue (the fat body, which is an analogue of the vertebrate liver) produces yolk proteins for developing oocytes. jh is important for the fat body to acquire competence for yolk protein production. however, the molecular mechanisms of how jh promotes mosquito rep ... | 2017 | 28446607 |
| species abundance and temporal variation of arbovirus vectors in brownsville, texas. | the recent outbreaks of the dengue fever and west nile viruses and the looming threats of the zika and chikungunya viruses highlight the importance of establishing effective, proactive arboviral surveillance in communities at high risk of transmission, such as those on the texas-mexico border. currently, there are no approved human vaccines available for these mosquito-borne diseases, so entomological control and case management are the only known methods for decreasing disease incidence. the pr ... | 2017 | 28444003 |
| dengue virus replicates and accumulates in aedes aegypti salivary glands. | dengue virus (denv) is an rna virus transmitted among humans by mosquito vectors, mainly aedes aegypti. denv transmission requires viral dissemination from the mosquito midgut to the salivary glands. during this process the virus undergoes several population bottlenecks, which are stochastic reductions in population size that restrict intra-host viral genetic diversity and limit the efficiency of natural selection. despite the implications for virus transmission and evolution, denv replication i ... | 2017 | 28431281 |
| yellow fever from angola and congo: a storm gathers. | in common with zika, chikungunya and dengue, yellow fever (yf) is an arthropod-borne flavivirus. it is transmitted between humans and from monkeys by mosquitoes of the aedes aegypti (its principal vector), haemogogus and albopictus varieties. three cycles of transmission may occur: urban; sylvatic; and intermediate. recently, sub-saharan africa has seen the resurgence of this neglected disease. the current yf outbreak in angola began in december 2015 in the capital luanda and by october 2016 the ... | 2017 | 28424031 |
| detection of a new pyrethroid resistance mutation (v410l) in the sodium channel of aedes aegypti: a potential challenge for mosquito control. | the yellow fever mosquito, aedes aegypti, particularly in neotropical regions, is the principal vector of dengue, yellow fever, zika and chikungunya viruses. pyrethroids remain one of the most used insecticides to control aedes mosquitoes, despite the development of pyrethroid resistance in many mosquito populations worldwide. here, we report a brazilian strain of a. aegypti with high levels (approximately 100-60,000 fold) of resistance to both type i and type ii pyrethroids. we detected two mut ... | 2017 | 28422157 |
| deploying dengue-suppressing wolbachia : robust models predict slow but effective spatial spread in aedes aegypti. | a novel strategy for controlling the spread of arboviral diseases such as dengue, zika and chikungunya is to transform mosquito populations with virus-suppressing wolbachia. in general, wolbachia transinfected into mosquitoes induce fitness costs through lower viability or fecundity. these maternally inherited bacteria also produce a frequency-dependent advantage for infected females by inducing cytoplasmic incompatibility (ci), which kills the embryos produced by uninfected females mated to inf ... | 2017 | 28411063 |
| severe dengue due to secondary hemophagocytic lymphohistiocytosis: a case study. | dengue, transmitted by the mosquito aedes aegypti affects millions of people worldwide every year. dengue induced hemophagocytic lymphohistiocytosis (hlh) is a serious condition and may prove fatal if not detected early and treated appropriately. diagnosis of hlh is challenging and usually missed as clinical and laboratory findings are nonspecific. moreover, the pathophysiology of the systemic inflammatory response syndrome and/or sepsis is remarkably similar to hlh. secondary hlh following infe ... | 2017 | 28409119 |
| vector competence and innate immune responses to dengue virus infection in selected laboratory and field-collected stegomyia aegypti (= aedes aegypti). | control of dengue virus (denv) transmission, primarily based on strategies to reduce populations of the principle vector stegomya aegypti (= aedes aegypti) (diptera: culicidae), is difficult to sustain over time. other potential strategies aim to manipulate characteristics such as vector competence (vc), the innate capacity of the vector to transmit the virus. previous studies have identified genetic factors, including differential expression of apoptosis-related genes, associated with the refra ... | 2017 | 28407282 |
| advances in vector control science: rear-and-release strategies show promise… but don't forget the basics. | both chikungunya and zika viruses have recently swept from africa across the pacific to the americas, causing major outbreaks of disease in humans. in the meantime, dengue epidemics continue throughout the tropics. traditional vector control programs based on strategies from 1950s and 1960s have been relatively ineffective in combating recent epidemics. in response, new methods involving the rearing and releasing of large numbers of mosquitoes to eliminate or modify local aedes populations are b ... | 2017 | 28403439 |
| aedes aegypti (diptera: culicidae) abundance model improved with relative humidity and precipitation-driven egg hatching. | we propose an improved aedes aegypti (l.) abundance model that takes into account the effect of relative humidity (rh) on adult survival, as well as rainfall-triggered egg hatching. the model uses temperature-dependent development rates described in the literature as well as documented estimates for mosquito survival in environments with high rh, and for egg desiccation. we show that combining the two additional components leads to better agreement with surveillance trap data and with dengue inc ... | 2017 | 28402546 |
| repellency of 29 synthetic and natural commercial topical insect repellents against aedes aegypti (diptera: culicidae) in central mexico. | in mexico, the use of repellents to prevent insects from landing and biting is a common practice. however, variation in the efficiency of natural and synthetic repellents has been observed. in this study, we evaluated the repellency and protection time of 16 synthetic and 13 natural-based commercial products against aedes aegypti (l.) from an endemic dengue area (jojutla, morelos) in central mexico. the "arm exposure" cage test was used to assess the efficacy of the repellents. tests were conduc ... | 2017 | 28402436 |
| why is aedes aegypti linnaeus so successful as a species? | diseases transmitted by mosquitoes impose enormous burden towards human morbidity and mortality. over the last three decades, brazil has suffered from severe dengue epidemics. in september 2014, this situation is further complicated by the introduction of two other viruses, zika and chikungunya, placing brazil in a triple epidemic. in this article, we discuss the biology of aedes aegypti linnaeus, and the principal initiatives currently used to control mosquito populations and the diseases they ... | 2017 | 28401481 |
| dengue vectors in urban and suburban assam, india: entomological observations. | dengue is rapidly becoming established in north-east india and spreading, on account of rapid urbanization and population movement, with reported morbidity and attributable death cases. this study aims to determine the seasonal abundance of aedes (stegomyia) albopictus and aedes (stegomyia) aegypti in guwahati metropolis and suburban settlements; to characterize the breeding resources for these mosquitoes; and to ascertain the status of their susceptibility to adulticides and larvicides. | 2017 | 28607255 |
| zika virus alters the microrna expression profile and elicits an rnai response in aedes aegypti mosquitoes. | zika virus (zikv), a flavivirus transmitted primarily by aedes aegypti, has recently spread globally in an unprecedented fashion, yet we have a poor understanding of host-microbe interactions in this system. to gain insights into the interplay between zikv and the mosquito, we sequenced the small rna profiles in zikv-infected and non-infected ae. aegypti mosquitoes at 2, 7 and 14 days post-infection. zika induced an rnai response in the mosquito with virus-derived short interfering rnas and piwi ... | 2017 | 28715413 |
| biopolymer zein-coated gold nanoparticles: synthesis, antibacterial potential, toxicity and histopathological effects against the zika virus vector aedes aegypti. | the control of multidrug-resistant bacteria as well as insect pests and vectors is timely and important now a days. the present study was designed to evaluate the in vitro antibacterial, antibiofilm and mosquito larvicidal effects of gold nanoparticles synthesized using the zein biopolymer (ze-aunps) against gram positive (bacillus pumilus and bacillus subtilis), gram negative (shigella sonnei and pseudomonas aeruginosa) bacteria and third instar larvae of the dengue and zika virus vector aedes ... | 2017 | 28654862 |
| cell fusing agent virus and dengue virus mutually interact in aedes aegypti cell lines. | the genus flavivirus contains more than 70 single-stranded, positive-sense arthropod-borne rna viruses. some flaviviruses are particularly medically important to humans and other vertebrates including dengue virus (denv), west nile virus, and yellow fever virus. these viruses are transmitted to vertebrates by mosquitoes and other arthropod species. mosquitoes are also infected by insect-specific flaviviruses (isfs) that do not appear to be infective to vertebrates. cell fusing agent virus (cfav) ... | 2017 | 28761113 |
| experimental infection with and maintenance of cell fusing agent virus (flavivirus) in aedes aegypti. | during the past two decades, there has been a dramatic increase in the recognition and characterization of novel insect-specific flaviviruses (isfvs). some of these agents are closely related to important mosquito-borne flavivirus pathogens. results of experimental studies suggest that mosquitoes and mosquito cell cultures infected with some isfvs are refractory to superinfection with related flavivirus pathogens; and it has been proposed that isfvs potentially could be used to alter the vector ... | 2017 | 28719335 |
| activation of aedes aegypti prophenoloxidase-3 and its role in the immune response against entomopathogenic fungi. | serine protease cascade-mediated melanization is an important innate immune response in insects and crustaceans, which involves the proteolytic activation of prophenoloxidase (ppo). in this study, we investigated the role of aedes aegypti ppo3 in antifungal immune defence. we expressed and purified recombinant ppo3 (rppo3) in escherichia coli and demonstrated that rppo3 was activated by ethanol and, to a lesser extent, by cetylpyridinium chloride. in the presence of cu(2+) , rppo3 exhibited enzy ... | 2017 | 28556276 |
| understanding the role of micrornas in the interaction of aedes aegypti mosquitoes with an insect-specific flavivirus. | the flavivirus genus contains some of the most prevalent vector-borne viruses, such as the dengue, zika and yellow fever viruses that cause devastating diseases in humans. however, the insect-specific clade of flaviviruses is restricted to mosquito hosts, albeit they have retained the general features of the genus, such as genome structure and replication. the interactions between insect-specific flaviviruses (isfs) and their mosquito hosts are largely unknown. pathogenic flaviviruses are known ... | 2017 | 28699859 |
| transposition of mutator-like transposable elements (mules) resembles hat and transib elements and v(d)j recombination. | mutator-like transposable elements (mules) are widespread across fungal, plant and animal species. despite their abundance and importance as genetic tools in plants, the transposition mechanism of the mule superfamily was previously unknown. discovery of the muta1 element from aedes aegypti and its successful transposition in yeast facilitated the characterization of key steps in muta1 transposition. here we show that purified transposase binds specifically to the muta1 ends and catalyzes excisi ... | 2017 | 28482040 |
| ascogregarina taiwanensis infection in aedes aegypti and aedes albopictus in santa catarina, south brazil. | this study registers ascogregarina spp. infection in field populations of aedes aegypti and aedes albopictus in a subtropical region of brazil. | 2017 | 28562761 |
| molecular characterization of the gene profile of bacillus thuringiensis berliner isolated from brazilian ecosystems and showing pathogenic activity against mosquito larvae of medical importance. | the occurrence of aedes aegypti, culex quinquefasciatus, and mosquitoes of the genus anopheles potentiate the spread of several diseases, such as dengue, zika, chikungunya, urban yellow fever, filariasis, and malaria, a situation currently existing in brazil and in latin america. control of the disease vectors is the most effective tool for containing the transmission of the pathogens causing these diseases, and the bacterium bacillus thuringiensis var. israelensis has been widely used and has s ... | 2017 | 28823909 |
| mosquitocidal potential of silver nanoparticles synthesized using local isolates of bacillus thuringiensis subsp. israelensis and their synergistic effect with a commercial strain of b. thuringiensis subsp. israelensis. | control of larval stages of aedes aegypti is considered an effective approach for preventing outbreaks of dengue fever. in this work, silver nanoparticles (ag nps) were synthesized using the supernatant and insecticidal proteins from local isolates of bacillus thuringiensis subsp. israelensis (bti). mosquitocidal activity assays against a. aegypti larvae revealed that the highest toxicity was obtained from the ag nps synthesized using supernatant of bti k55 and the inclusion proteins of bti k46 ... | 2017 | 28754251 |
| toward mosquito control with a green alga: expression of cry toxins of bacillus thuringiensis subsp. israelensis (bti) in the chloroplast of chlamydomonas. | we are developing chlamydomonas strains that can be used for safe and sustainable control of mosquitoes, because they produce proteins from bacillus thuringiensis subsp. israelensis (bti) in the chloroplast. chlamydomonas has a number of advantages for this approach, including genetic controls that are not generally available with industrial algae. the bti toxin has been used for mosquito control for > 30 years and does not engender resistance; it contains three cry proteins, cry4aa (135 kda), c ... | 2017 | 28713202 |
| molecular characterization of indigenous bacillus thuringiensis strains isolated from kashmir valley. | bacillus thuringiensis (bt) being an eco-friendly bioinsecticide is effectively used in pest management strategies and, therefore, isolation and identification of new strains effective against a broad range of target pests is important. in the present study, new indigenous b. thuringiensis strains were isolated and investigated so that these could be used as an alternative and/or support the current commercial strains/cry proteins in use. for this, 159 samples including soil, leaf and spider web ... | 2017 | 28597156 |
| functional characterization of aedes aegypti alkaline phosphatase alp1 involved in the toxicity of cry toxins from bacillus thuringiensis subsp. israelensis and jegathesan. | presently three major groups of proteins from aedes aegypti, cadherin, alkaline phosphatases (alp) and aminopeptidases n (apn), have been identified as cry11aa toxin receptors. to further characterize their role on toxicity, transgenic mosquitoes with silenced aedes cadherin expression were previously generated and the role of cadherin in mediating the toxicity of four different mosquitocidal toxins (cry11aa, cry11ba, cry4aa and cry4ba) was demonstrated. here, we investigated the role of another ... | 2017 | 28587836 |
| evaluating the vector control potential of the in2care® mosquito trap against aedes aegypti and aedes albopictus under semifield conditions in manatee county, florida. | successful integrated vector management programs may need new strategies in addition to conventional larviciding and adulticiding strategies to target aedes aegypti and ae. albopictus, which can develop in small, often cryptic, artificial and natural containers. the in2care® mosquito trap was recently developed to target and kill larval and adult stages of these invasive container-inhabiting aedes mosquitoes by utilizing autodissemination. gravid females that visit the trap pick up pyriproxyfen ... | 2017 | 28854105 |
| mosquitoes can harbour yeasts of clinical significance and contribute to their environmental dissemination. | there is still a lack of studies on fungal microbiota in mosquitoes, compared with the number available on bacterial microbiota. this study reports the identification of yeasts of clinical significance in laboratory mosquito species: anopheles gambiae, anopheles stephensi, culex quinquefasciatus, aedes albopictus and aedes aegypti. among the yeasts isolated, they focused on the opportunistic pathogen candida parapsilosis, since there is a need to better understand breakthrough candidaemia with r ... | 2017 | 28714286 |
| vector competence of populations of aedes aegypti from three distinct cities in kenya for chikungunya virus. | in april, 2004, chikungunya virus (chikv) re-emerged in kenya and eventually spread to the islands in the indian ocean basin, south-east asia, and the americas. the virus, which is often associated with high levels of viremia in humans, is mostly transmitted by the urban vector, aedes aegypti. the expansion of chikv presents a public health challenge both locally and internationally. in this study, we investigated the ability of ae. aegypti mosquitoes from three distinct cities in kenya; mombasa ... | 2017 | 28820881 |
| co-infection of mosquitoes with chikungunya and dengue viruses reveals modulation of the replication of both viruses in midguts and salivary glands of aedes aegypti mosquitoes. | arthropod-borne virus (arbovirus) infections cause several emerging and resurgent infectious diseases in humans and animals. chikungunya-affected areas often overlap with dengue-endemic areas. concurrent dengue virus (denv) and chikungunya virus (chikv) infections have been detected in travelers returning from regions of endemicity. chikv and denv co-infected aedes albopictus have also been collected in the vicinity of co-infected human cases, emphasizing the need to study co-infections in mosqu ... | 2017 | 28777313 |
| high-resolution structure of a kazal-type serine protease inhibitor from the dengue vector aedes aegypti. | blood-feeding exoparasites are rich sources of protease inhibitors, and the mosquito aedes aegypti, which is a vector of dengue virus, yellow fever virus, chikungunya virus and zika virus, is no exception. aati is a single-domain, noncanonical kazal-type serine proteinase inhibitor from a. aegypti that recognizes both digestive trypsin-like serine proteinases and the central protease in blood clotting, thrombin, albeit with an affinity that is three orders of magnitude lower. here, the 1.4 å res ... | 2017 | 28777090 |
| evidence of dengue and chikungunya virus co-infection and circulation of multiple dengue serotypes in a recent indian outbreak. | in india, dengue endemic areas overlap with chikungunya-affected areas and both the viruses are transmitted by same vector, aedes aegypti - thereby increasing opportunity of co-infection by both viruses. present study was carried out to understand the denv-chikv infection dynamics during recent outbreaks in eastern india (west bengal state) and its implication on disease manifestations. blood was collected from 326 symptomatic febrile patients. patients' serum was subjected to serological diagno ... | 2017 | 28756561 |
| transmission risk of two chikungunya lineages by invasive mosquito vectors from florida and the dominican republic. | between 2014 and 2016 more than 3,800 imported human cases of chikungunya fever in florida highlight the high risk for local transmission. to examine the potential for sustained local transmission of chikungunya virus (chikv) in florida we tested whether local populations of aedes aegypti and aedes albopictus show differences in susceptibility to infection and transmission to two emergent lineages of chikv, indian ocean (ioc) and asian genotypes (ac) in laboratory experiments. all examined popul ... | 2017 | 28749964 |
| assessment of the probability of autochthonous transmission of chikungunya virus in canada under recent and projected climate change. | chikungunya virus (chikv) is a reemerging pathogen transmitted by aedes aegypti and aedes albopictus mosquitoes. the ongoing caribbean outbreak is of concern due to the potential for infected travelers to spread the virus to countries where vectors are present and the population is susceptible. although there has been no autochthonous transmission of chikv in canada, there is concern that both ae. albopictus and chikv will become established, particularly under projected climate change. we devel ... | 2017 | 28731409 |
| the medicinal chemistry of chikungunya virus. | arthropod-borne viruses (arboviruses) are an important threat to human and animal health globally. among these, zoonotic diseases account for billions of cases of human illness and millions of deaths every year, representing an increasing public health problem. chikungunya virus belongs to the genus alphavirus of the family togariridae, and is transmitted mainly by the bite of female mosquitoes of the aedes aegypti and/or a. albopictus species. the focus of this review will be on the medicinal c ... | 2017 | 28689975 |
| high risk for chikungunya virus to initiate an enzootic sylvatic cycle in the tropical americas. | chikungunya virus (chikv) has dispersed in the americas since 2013, and its range of distribution has overlapped large forested areas. herein, we assess vector competence of two sylvatic neotropical mosquito species, haemagogus leucocelaenus and aedes terrens, to evaluate the risk of chikv to initiate a sylvatic cycle in the continent. | 2017 | 28662031 |
| first report of naturally infected aedes aegypti with chikungunya virus genotype ecsa in the americas. | the worldwide expansion of new emergent arboviruses such as chikungunya and zika reinforces the importance in understanding the role of mosquito species in spreading these pathogens in affected regions. this knowledge is essential for developing effective programs based on species specificity to avoid the establishment of endemic transmission cycles sustained by the identified local vectors. although the first autochthonous transmission of chikungunya virus was described in 2014 in the north of ... | 2017 | 28614394 |
| immunoglobulin-responsive chikungunya encephalitis: two case reports. | chikungunya virus is an alphavirus transmitted by the mosquito aedes, mainly aedes aegypti and aedes albopictus, that can cause acute illness, mostly self-limited, characterized by fever, maculopapular rash, and disabling polyarthritis/arthralgia, with an incubation period of 1 to 12 days. chikungunya was largely regarded as a non-fatal and self-limited disease, but recently, serious cases have been reported including some with severe involvement of the nervous system, such as meningoencephaliti ... | 2017 | 28577289 |
| mosquito co-infection with zika and chikungunya virus allows simultaneous transmission without affecting vector competence of aedes aegypti. | zika virus (zikv) and chikungunya virus (chikv) are highly pathogenic arthropod-borne viruses that are currently a serious health burden in the americas, and elsewhere in the world. zikv and chikv co-circulate in the same geographical regions and are mainly transmitted by aedes aegypti mosquitoes. there is a growing number of case reports of zikv and chikv co-infections in humans, but it is uncertain whether co-infection occurs via single or multiple mosquito bites. here we investigate the poten ... | 2017 | 28570693 |
| wmel limits zika and chikungunya virus infection in a singapore wolbachia-introgressed ae. aegypti strain, wmel-sg. | zika (zikv) and chikungunya (chikv) viruses are emerging aedes-borne viruses that are spreading outside their known geographic range and causing wide-scale epidemics. it has been reported that these viruses can be transmitted efficiently by ae. aegypti. recent studies have shown that ae. aegypti when transinfected with certain wolbachia strains shows a reduced replication and dissemination of dengue (denv), chikungunya (chikv), and yellow fever (yfv) viruses. the aim of this study was to determi ... | 2017 | 28542240 |
| [arboviruses also have an american dream]. | some arboviruses that originated in the old world have been introduced by humans into the american continent. the first of them was the yellow fever virus, coming from the west african coast with slaves in the 17th-19th centuries, followed by dengue viruses, which were always prevalent within the americas. next was thewest nile virus, introduced in new york in 1999, that spread in only a few years over the whole continent. then, chikungunya virus arrived on saint martin island in 2013 after its ... | 2017 | 28509947 |
| the midgut transcriptome of aedes aegypti fed with saline or protein meals containing chikungunya virus reveals genes potentially involved in viral midgut escape. | the mosquito aedes aegypti is the primary vector for medically important arthropod-borne viruses, including chikungunya virus (chikv). following oral acquisition, an arbovirus has to persistently infect several organs in the mosquito before becoming transmissible to another vertebrate host. a major obstacle an arbovirus has to overcome during its infection cycle inside the mosquito is the midgut escape barrier, representing the exit mechanism arboviruses utilize when disseminating from the midgu ... | 2017 | 28506207 |
| clinical features and neurologic complications of children hospitalized with chikungunya virus in honduras. | the first case of chikungunya virus in honduras was identified in 2014. the virus has spread widely across honduras via the aedes aegypti mosquito, leading to an outbreak of chikungunya virus (chikv) in 2015 that significantly impacted children. a retrospective chart review of 235 children diagnosed with chikv and admitted to the national autonomous university of honduras hospital escuela (hospital escuela) in tegucigalpa, honduras, was accomplished with patients who were assessed for clinical f ... | 2017 | 28459170 |
| co-circulation of dengue virus serotypes with chikungunya virus in madhya pradesh, central india. | dengue and chikungunya present with very similar signs and symptoms in the initial stage of illness and so it is difficult to distinguish them clinically. both are transmitted by aedes aegypti and aedes albopictus mosquitoes. this study was conducted with the aim to explore the co-circulation of dengue and chikungunya viruses in central india. | 2017 | 28607252 |
| ensuring successful introduction of wolbachia in natural populations of aedes aegypti by means of feedback control. | the control of the spread of dengue fever by introduction of the intracellular parasitic bacterium wolbachia in populations of the vector aedes aegypti, is presently one of the most promising tools for eliminating dengue, in the absence of an efficient vaccine. the success of this operation requires locally careful planning to determine the adequate number of individuals carrying the wolbachia parasite that need to be introduced into the natural population. the introduced mosquitoes are expected ... | 2017 | 28856446 |
| influence of the mixtures composed of slow-release insecticide formulations against aedes aegypti mosquito larvae reared in pond water. | vector borne diseases remain the major source of illness and death worldwide. aedes aegypti is the primary carrier of dengue and dengue haemorrhagic fever in many developing countries in the tropical world. because a. aegypti populations are becoming more and more resistant to conventional and non conventional insecticides, alternative strategies have to be rapidly implemented in the future for dengue vector control. the present study aimed to evaluate the larvicidal efficacy of slow-release for ... | 2017 | 28855810 |
| human movement, cooperation and the effectiveness of coordinated vector control strategies. | vector-borne disease transmission is often typified by highly focal transmission and influenced by movement of hosts and vectors across different scales. the ecological and environmental conditions (including those created by humans through vector control programmes) that result in metapopulation dynamics remain poorly understood. the development of control strategies that would most effectively limit outbreaks given such dynamics is particularly urgent given the recent epidemics of dengue, chik ... | 2017 | 28855386 |
| a statewide survey for container-breeding mosquitoes in mississippi. | container-breeding mosquitoes are important in public health due to outbreaks of zika, chikungunya, and dengue viruses. this paper documents the distribution of container-breeding mosquito species in mississippi, with special emphasis on the genus aedes. five sites in each of the 82 mississippi counties were sampled monthly between may 1 and august 31, 2016, and 50,109 mosquitoes in 14 species were collected. the most prevalent and widely distributed species found was ae. albopictus, being found ... | 2017 | 28854113 |
| adulticidal susceptibility evaluation of aedes albopictus using new diagnostic doses in penang island, malaysia. | insecticide-based vector control approaches are facing challenges due to the development of resistance in vector mosquitoes. therefore, a proper resistance surveillance program using baseline lethal concentrations is crucial for resistance management strategies. currently, the world health organization's (who) diagnostic doses established for aedes aegypti and anopheles species are being used to study the resistance status of aedes albopictus. in this study, we established the diagnostic doses f ... | 2017 | 28854111 |
| toxicological effects of chemical constituents from piper against the environmental burden aedes aegypti liston and their impact on non-target toxicity evaluation against biomonitoring aquatic insects. | dengue is the most rapidly spreading mosquito-borne viral disease in the world. the mosquito, aedes aegypti, also spreads yellow fever, chikungunya, and zika virus. as the primary vector for dengue, ae. aegypti now occurs in over 20 countries and is a serious concern with reports of increasing insecticide resistance. developing new treatments to manage mosquitoes are needed. formulation of crude volatile oil from piper betle leaves (pb-cvo) was evaluated as a potential treatment which showed lar ... | 2017 | 28852982 |
| the elimination of the dengue vector, aedes aegypti, from brisbane, australia: the role of surveillance, larval habitat removal and policy. | aedes aegypti (l.) (diptera: culicidae) is a highly invasive mosquito whose global distribution has fluctuated dramatically over the last 100 years. in australia the distribution of ae. aegypti once spanned the eastern seaboard, for 3,000 km north to south. however, during the 1900s this distribution markedly reduced and the mosquito disappeared from its southern range. numerous hypotheses have been proffered for this retraction, however quantitative evidence of the mechanisms driving the disapp ... | 2017 | 28846682 |
| zika, chikungunya, and other emerging vector-borne viral diseases. | arthropod-borne viruses (arboviruses) have a long history of emerging to infect humans, but during recent decades, they have been spreading more widely and affecting larger populations. this is due to several factors, including increased air travel and uncontrolled mosquito vector populations. emergence can involve simple spillover from enzootic (wildlife) cycles, as in the case of west nile virus accompanying geographic expansion into the americas; secondary amplification in domesticated animal ... | 2017 | 28846489 |
| sero-surveillance of dengue in the city lahore, pakistan. | to conduct sero-surveillance of dengue cases, and to find out the demographic and socio-economic status of dengue patients. | 2017 | 28839300 |
| carryover effects of larval exposure to different environmental bacteria drive adult trait variation in a mosquito vector. | conditions experienced during larval development of holometabolous insects can affect adult traits, but whether differences in the bacterial communities of larval development sites contribute to variation in the ability of insect vectors to transmit human pathogens is unknown. we addressed this question in the mosquito aedes aegypti, a major arbovirus vector breeding in both sylvatic and domestic habitats in sub-saharan africa. targeted metagenomics revealed differing bacterial communities in th ... | 2017 | 28835919 |