Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| metazoan ribosome inactivating protein encoding genes acquired by horizontal gene transfer. | ribosome inactivating proteins (rips) are rna n-glycosidases that depurinate a specific adenine residue in the conserved sarcin/ricin loop of 28s rrna. these enzymes are widely distributed among plants and their presence has also been confirmed in several bacterial species. recently, we reported for the first time in silico evidence of rip encoding genes in metazoans, in two closely related species of insects: aedes aegypti and culex quinquefasciatus. here, we have experimentally confirmed the p ... | 2017 | 28500327 |
| larval interference competition between the native neotropical mosquito limatus durhamii and the invasive aedes aegypti improves the fitness of both species. | interspecific competition with native species during biological invasions can sometimes limit alien expansion. we aimed to determine the potential ecological effects of limatus durhamii theobald 1901, a native neotropical mosquito (diptera: culicidae) species, on the invasive species aedes (stegomyia) aegypti (linnaeus 1762) that breeds in the same artificial water containers. development time and adult dry mass were measured in 3 rearing conditions: control (a single larva), intraspecific compe ... | 2017 | 28497885 |
| assessing the effect of selection with deltamethrin on biological parameters and detoxifying enzymes in aedes aegypti (l.). | resistance to insecticides through one or several mechanisms has a cost for an insect in various parameters of its biological cycle. the present study evaluated the effect of deltamethrin on detoxifying enzymes and biological parameters in a population of aedes aegypti selected for 15 generations. the enzyme activities of alpha- and beta-esterases, mixed-function oxidases and glutathione-s-transferases were determined during selection, along with biological parameters. | 2017 | 28485040 |
| what does heat tell a mosquito? characterization of the orientation behaviour of aedes aegypti towards heat sources. | the use of heat as a cue for the orientation of haematophagous insects towards hot-blooded hosts has been acknowledged for many decades. in mosquitoes, thermoreception has been studied at the molecular, physiological and behavioural levels, and the response to heat has been evaluated in multimodal contexts. however, a direct characterization of how these insects evaluate thermal sources is still lacking. in this study we characterize aedes aegypti thermal orientation using a simple dual choice p ... | 2017 | 28479122 |
| eco-friendly synthesis of silver nanoparticles and its larvicidal property against fourth instar larvae of aedes aegypti. | in this study, larvicidal activity of silver nanoparticles (agnps) synthesised using apple extract against fourth instar larvae of aedes aegypti was determined. as a result, the agnps showed moderate larvicidal effects against ae. aegypti larvae (lc50 = 15.76 ppm and lc90 = 27.7 ppm). in addition, comparison of larvicidal activity performance of agnps at high concentration prepared using two different methods showed that ae. aegypti larvae was fully eliminated within the duration of 2.5 h. from ... | 2017 | 28476997 |
| photo-induced and phytomediated synthesis of silver nanoparticles using derris trifoliata leaf extract and its larvicidal activity against aedes aegypti. | due to the increased development of resistance of vectors against synthetic insecticides and chemical drugs, plant based insecticides serve as promising biocontrol agents for effective vector control. green approach for the synthesis of nanoparticles has been attained using environmentally safe, non-toxic plant extracts. the present study was aimed to investigate the potent larvicidal activity of silver nanoparticles (agnps) produced by derris trifoliata leaf extract in relation to the various c ... | 2017 | 28460330 |
| allergens involved in the cross-reactivity of aedes aegypti with other arthropods. | cross-reactivity between aedes aegypti and mites, cockroaches, and shrimp has been previously suggested, but the involved molecular components have not been fully described. | 2017 | 28434865 |
| n-aryl-n'-ethyleneaminothioureas effectively inhibit acetylcholinesterase 1 from disease-transmitting mosquitoes. | vector control of disease-transmitting mosquitoes by insecticides has a central role in reducing the number of parasitic- and viral infection cases. the currently used insecticides are efficient, but safety concerns and the development of insecticide-resistant mosquito strains warrant the search for alternative compound classes for vector control. here, we have designed and synthesized thiourea-based compounds as non-covalent inhibitors of acetylcholinesterase 1 (ache1) from the mosquitoes anoph ... | 2017 | 28433681 |
| identification of the septate junction protein gliotactin in the mosquito aedes aegypti: evidence for a role in increased paracellular permeability in larvae. | septate junctions (sjs) regulate paracellular permeability across invertebrate epithelia. however, little is known about the function of sj proteins in aquatic invertebrates. in this study, a role for the transmembrane sj protein gliotactin (gli) in the osmoregulatory strategies of larval mosquito (aedes aegypti) was examined. differences in gli transcript abundance were observed between the midgut, malpighian tubules, hindgut and anal papillae of a. aegypti, which are epithelia that participate ... | 2017 | 28432154 |
| allatotropin: a pleiotropic neuropeptide that elicits mosquito immune responses. | allatotropins (at) are neuropeptides with pleotropic functions on a variety of insect tissues. they affect processes such as juvenile hormone biosynthesis, cardiac rhythm, oviduct and hindgut contractions, nutrient absorption and circadian cycle. the present work provides experimental evidence that at elicits immune responses in two important mosquito disease vectors, anopheles albimanus and aedes aegypti. hemocytes and an immune-competent mosquito cell line responded to at by showing strong mor ... | 2017 | 28426765 |
| efficacy of some wearable devices compared with spray-on insect repellents for the yellow fever mosquito, aedes aegypti (l.) (diptera: culicidae). | the current zika health crisis in the americas has created an intense interest in mosquito control methods and products. mosquito vectors of zika are of the genus aedes, mainly the yellow fever mosquito, aedes aegypti. l. the use of repellents to alter mosquito host seeking behavior is an effective method for the prevention of mosquito-borne diseases. a large number of different spray-on repellents and wearable repellent devices are commercially available. the efficacies of many repellents are u ... | 2017 | 28423421 |
| habitat and density of oviposition opportunity influences aedes aegypti (diptera: culicidae) flight distance. | understanding the dispersal of aedes (aegypti (l.) diptera: culicidae) after consuming a potentially infectious bloodmeal is an important part of controlling the spread of the arboviruses it transmits. because of the impact on abundance, removal of oviposition sites is a key component of vector control. however, source reduction around a case may encourage dispersal of potentially infected vectors. we compare the effect of oviposition site availability on ae. aegypti dispersal behavior within 30 ... | 2017 | 28419273 |
| the impact of temperature and body size on fundamental flight tone variation in the mosquito vector aedes aegypti (diptera: culicidae): implications for acoustic lures. | aedes aegypti (l.) males use female flight tone as a means of mate localization. by playing the sound of a flying female, males can be attracted to a trap to monitor mosquito populations and the progress of transgenic male releases. however, the female flight tone used to attract males needs to be optimized to maximize trap effectiveness. the fundamental frequency of female flight tone could be influenced by both body size and ambient temperature. however, no analysis yet has considered both the ... | 2017 | 28402550 |
| immunohistochemical mapping and transcript expression of the gpa2/gpb5 receptor in tissues of the adult mosquito, aedes aegypti. | gpa2/gpb5 is a glycoprotein hormone found in most bilateral metazoans including the mosquito, aedes aegypti. to elucidate physiological roles and functions of gpa2/gpb5, we aim to identify prospective target tissues by examining the tissue- and sex-specific expression profile of its receptor, the leucine-rich repeat-containing g protein-coupled receptor 1 (lgr1) in the adult mosquito. western analyses using a heterologous system with cho-k1 cells, transiently expressing a. aegypti lgr1, yielded ... | 2017 | 28401307 |
| synthesis and insecticidal activity of novel pyrimidine derivatives containing urea pharmacophore against aedes aegypti. | aedes aegypti is a major mosquito vector for the transmission of serious diseases, especially dengue and yellow fever. more than 1 billion people in developing countries are at risk. the widespread and continual use of pesticides can lead to resistant mosquitoes. in order to maintain mosquito control gains, it is critical to develop and evaluate novel bioactive molecules that differ in mode of action from currently used products. | 2017 | 27448764 |
| changes in infestation sites of female aedes aegypti in northeast brazil. | we report the behavioral changes in use of oviposition sites in aedes aegypti in fortaleza, brazil. | 2017 | 27598639 |
| cost of intensive routine control and incremental cost of insecticide-treated curtain deployment in a setting with low aedes aegypti infestation. | information regarding the cost of implementing insecticide-treated curtains (itcs) is scarce. therefore, we evaluated the itc implementation cost, in addition to the costs of intensive conventional routine activities of the aedes control program in the city of guantanamo, cuba. | 2017 | 27598627 |
| sexual chemoecology of mosquitoes (diptera, culicidae): current knowledge and implications for vector control programs. | mosquitoes (diptera: culicidae) act as vectors of medical and veterinary importance, due to their ability to transmit many pathogens and parasites. renewed interest has been recently devoted to the potential of sterile insect technique (sit) for mosquito suppression. however, the success of the sit is mostly dependent on the ability of sterile males to compete for mates with the wild ones in the field. nevertheless, little is known on the sexual chemical ecology of mosquitoes, with special refer ... | 2017 | 27692501 |
| long-lasting permethrin-impregnated clothing: protective efficacy against malaria in hyperendemic foci, and laundering, wearing, and weathering effects on residual bioactivity after worst-case use in the rain forests of french guiana. | personal protective measures against hematophagous vectors constitute the first line of defense against arthropod-borne diseases. however, guidelines for the standardized testing and licensing of insecticide-treated clothing are still lacking. the aim of this study was to analyze the preventive effect of long-lasting polymer-coated permethrin-impregnated clothing (ptbdu) against malaria after exposure to high-level disease transmission sites as well as the corresponding loss of permethrin and bi ... | 2017 | 27942961 |
| impact of terminalia chebula retz. against aedes aegypti l. and non-target aquatic predatory insects. | aedes aegypti linn is one of the most important mosquito species. the vectors are responsible for causing deadly diseases like dengue and dengue hemorrhagic fever. several chemical pesticides used to control these dengue vectors caused severe toxic significances on human health and other non-target beneficial insects. therefore the current investigation has been made to access the bio-efficacy of the crude seed extracts of t. chebula against the dengue vector ae. aegypti. the gc-ms analysis of c ... | 2017 | 27940415 |
| field comparisons of the gravid aedes trap (gat) and bg-sentinel trap for monitoring aedes albopictus (diptera: culicidae) populations and notes on indoor gat collections in vietnam. | we report on the use of the gravid aedes trap (gat) as a surveillance device for aedes albopictus (skuse) relative to the bg-sentinel (bgs) trap in field studies conducted in trenton, nj, and on hammond island, queensland, australia. a parallel study conducted in nha trang, vietnam, assessed the use of the gat as an indoor surveillance device as well as the use of canola oil as a noninsecticide killing agent. in trenton and hammond island, the gat collected fewer male (0.40 ± 0.12 and 0.43 ± 0.3 ... | 2017 | 27707983 |
| salinity responsive aquaporins in the anal papillae of the larval mosquito, aedes aegypti. | the larvae of the mosquito, aedes aegypti normally inhabit freshwater (fw) where they face dilution of body fluids by osmotic influx of water. in response, the physiological actions of the anal papillae result in ion uptake while the malpighian tubules and rectum work in concert to excrete excess water. in an apparent paradox, the anal papillae express aquaporins (aqps) and are sites of water permeability which, if aqps are expressed by the epithelium, apparently exaggerates the influx of water ... | 2017 | 27642095 |
| the formulation of the essential oil of piper aduncum linnaeus (piperales: piperaceae) increases its efficacy as an insect repellent. | the essential oil (eo) of piper aduncum linnaeus, known as 'sireh lada' to locals malaysian, has the potential to be used as an alternative to synthetic insect repellents such as n,n-diethyl-meta-toluamide. however, the eo's efficacy as a repellent decreases after application due to the high volatility of its active ingredients. a number of studies have showed that optimizing the formulation of plant-based eos can improve their efficacy as repellents. the present study sought to evaluate the eff ... | 2017 | 27819201 |
| mosquito populations dynamics associated with climate variations. | mosquitoes are responsible for the transmission of numerous serious pathogens. members of the aedes and culex genera, which include many important vectors of mosquito-borne diseases, are highly invasive and adapted to man-made environments. they are spread around the world involuntarily by humans and are highly adapted to urbanized environments, where they are exposed to climate-related abundance drivers. we investigated culicidae fauna in two urban parks in the city of são paulo to analyze the ... | 2017 | 27810426 |
| essential oils enhance the toxicity of permethrin against aedes aegypti and anopheles gambiae. | insecticide resistance and growing public concern over the safety and environmental impacts of some conventional insecticides have resulted in the need to discover alternative control tools. naturally occurring botanically-based compounds are of increased interest to aid in the management of mosquitoes. susceptible strains of aedes aegypti (linnaeus) (diptera: culicidae) and anopheles gambiae (meigen) (diptera: culicidae) were treated with permethrin, a common type-i synthetic pyrethroid, using ... | 2017 | 27800630 |
| how much does inbreeding reduce heterozygosity? empirical results from aedes aegypti. | deriving strains of mosquitoes with reduced genetic variation is useful, if not necessary, for many genetic studies. inbreeding is the standard way of achieving this. full-sib inbreeding the mosquito aedes aegypti for seven generations reduced heterozygosity to 72% of the initial heterozygosity in contrast to the expected 13%. this deviation from expectations is likely due to high frequencies of deleterious recessive alleles that, given the number of markers studied (27,674 single nucleotide pol ... | 2017 | 27799643 |
| repellency and larvicidal activity of essential oils from xylopia laevigata, xylopia frutescens, lippia pedunculosa, and their individual compounds against aedes aegypti linnaeus. | in order to find new alternatives for vector control and personal protection, we evaluated the larvicidal and repellent activity of essentials oils from plants found in the northeast of brazil against aedes aegypti linnaeus mosquitoes. the plants tested include xylopia laevigata, xylopia frutescens, and lippia pedunculosa and their major compounds, piperitenone oxide, and (r)-limonene. the essential oil of l. pedunculosa and its major volatile compounds were shown to be toxic for ae. aegypti lar ... | 2017 | 27844468 |
| dynamic expression of genes encoding subunits of inward rectifier potassium (kir) channels in the yellow fever mosquito aedes aegypti. | inward rectifier potassium (kir) channels play fundamental roles in neuromuscular, epithelial, and endocrine function in mammals. recent research in insects suggests that kir channels play critical roles in the development, immune function, and excretory physiology of fruit flies and/or mosquitoes. moreover, our group has demonstrated that mosquito kir channels may serve as valuable targets for the development of novel insecticides. here we characterize the molecular expression of 5 mrnas encodi ... | 2017 | 27836744 |
| establishment of aedes aegypti (l.) in mountainous regions in mexico: increasing number of population at risk of mosquito-borne disease and future climate conditions. | the study was conducted in the central region of veracruz mexico, in the metropolitan area of xalapa. it is a mountainous area where aedes aegypti (l.) is not currently endemic. an entomological survey was done along an elevation gradient using the ae. aegypti occurrences at different life cycle stages. seven sites were sampled and a total of 24 mosquito species were recorded: 9 species were found in urban areas, 18 in non-urban areas with remnant vegetation, and 3 occurred in both environments. ... | 2017 | 27863974 |
| aedes aegypti rhesus glycoproteins contribute to ammonia excretion by larval anal papillae. | in larval aedes aegypti, transcripts of the rhesus-like glycoproteins aerh50-1 and aerh50-2 have been detected in the anal papillae, sites of ammonia (nh3/nh4(+)) excretion; however, these putative ammonia transporters have not been previously localized or functionally characterized. in this study, we show that the aerh50s co-immunolocalize with apical v-type h(+)-atpase as well as with basal na(+)/k(+)-atpase in the epithelium of anal papillae. the double-stranded rna-mediated knockdown of aerh ... | 2017 | 27885043 |
| long-term spatio-temporal dynamics of the mosquito aedes aegypti in temperate argentina. | buenos aires city is located near the southern limit of the distribution of aedes aegypti (diptera: culicidae). this study aimed to assess long-term variations in the abundance of ae. aegypti in buenos aires in relation to changes in climatic conditions. ae. aegypti weekly oviposition activity was analyzed and compared through nine warm seasons from 1998 to 2014, with 200 ovitraps placed across the whole extension of the city. the temporal and spatial dynamics of abundances were compared among s ... | 2017 | 27876100 |
| salinity alters snakeskin and mesh transcript abundance and permeability in midgut and malpighian tubules of larval mosquito, aedes aegypti. | this study examined the distribution and localization of the septate junction (sj) proteins snakeskin (ssk) and mesh in osmoregulatory organs of larval mosquito (aedes aegypti), as well as their response to altered environmental salt levels. ssk and mesh transcripts and immunoreactivity were detected in tissues of endodermal origin such as the midgut and malpighian tubules of a. aegypti larvae, but not in ectodermally derived hindgut and anal papillae. immunolocalization of ssk and mesh in the m ... | 2017 | 27988380 |
| diverse subcellular localizations of the insect cmp-sialic acid synthetases. | the occurrence and biological importance of sialic acid (sia) and its metabolic enzymes in insects have been studied using drosophila melanogaster. the most prominent feature of d. melanogaster cmp-sia synthetase (dmcss) is its golgi-localization, contrasted with nuclear localization of vertebrate csss. however, it remains unclear if the golgi-localization is common to other insect csss and why it happens. to answer these questions, aedes aegypti (mosquito) css (aacss) and tribolium castaneum (b ... | 2017 | 27986833 |
| expression of heat shock proteins (hsps) in aedes aegypti (l) and aedes albopictus (skuse) (diptera: culicidae) larvae in response to thermal stress. | climatic changes are responsible, to a certain extent for the occurrence and spread of arboviral pathogens world over. temperature is one of the important abiotic factors influencing the physiological processes of mosquitoes. several genes of heat shock protein (hsp) families are known to be expressed in mosquitoes, which aid in overcoming stress induced by elevated temperature. in order to understand expression of hsp family genes in the andaman population of aedes aegypti and aedes albopictus, ... | 2017 | 28024869 |
| mosquito repellent activity of volatile oils from selected aromatic plants. | essential oils from fresh leaves of four aromatic plants viz., ocimum sanctum, mentha piperita, eucalyptus globulus and plectranthus amboinicus were extracted by hydrodistillation. the test solutions were prepared as 20% essential oil in ethanol and positive control as 20% deet in ethanol. essential oil blend was prepared as 5% concentration. nulliparous, 3-5-day-old female adult aedes aegypti mosquitoes were used for repellency screening as per icmr protocol. the study showed that the repellenc ... | 2017 | 28013374 |
| influence of the length of storage on aedes aegypti (diptera: culicidae) egg viability. | aedes aegypti (l.) is one of the most important arboviral vectors worldwide. vector control is targeted at immature and adult stages; however, eggs are resistant to desiccation and may repopulate treated areas long after treatment ceases. we investigated the effect of age on ae. aegypti egg hatching rates using newly colonized populations (f2) from an arid region. we found a strongly negative association where older eggs had lower hatch rates. the capacity of eggs to survive for long periods of ... | 2017 | 28011737 |
| indoor resting behavior of aedes aegypti (diptera: culicidae) in acapulco, mexico. | the markedly anthropophilic and endophilic behaviors of aedes aegypti (l.) make it a very efficient vector of dengue, chikungunya, and zika viruses. although a large body of research has investigated the immature habitats and conditions for adult emergence, relatively few studies have focused on the indoor resting behavior and distribution of vectors within houses. we investigated the resting behavior of ae. aegypti indoors in 979 houses of the city of acapulco, mexico, by performing exhaustive ... | 2017 | 28011725 |
| toxicity and synergistic activities of chalcones against aedes aegypti (diptera: culicidae) and drosophila melanogaster (diptera: drosophilidae). | mosquito-borne illnesses are of great concern throughout the world, and chemical insecticides are commonly employed to decrease mosquito populations. however, the developmental insecticide pipeline for vector control has primarily been filled by repurposed agricultural products, and is hampered by their widespread use and insecticide resistance. the present study was performed in the search for new chemical insecticides or insecticide synergists. screening of 31 chalcone analogs was performed us ... | 2017 | 28011724 |
| exon-enriched libraries reveal large genic differences between aedes aegypti from senegal, west africa, and populations outside africa. | aedes aegypti is one of the most studied mosquito species, and the principal vector of several arboviruses pathogenic to humans. recently failure to oviposit, low fecundity, and poor egg-to-adult survival were observed when ae. aegypti from senegal (senaae) west africa were crossed with ae. aegypti (aaa) from outside of africa, and in senaae intercrosses. fluorescent in situ hybridization analyses indicated rearrangements on chromosome 1, and pericentric inversions on chromosomes 2 and 3. herein ... | 2017 | 28007834 |
| in vitro evaluation of the effect of botanical formulations used in the control of aedes aegypti l. (diptera: culicidae) on liver enzymes. | dengue fever is a viral disease transmitted by the aedes aegypti linn. (1792) (diptera: culicidae) mosquito, which is endemic in several regions of brazil. alternative methods for the control of the vector include botanical insecticides, which offer advantages such as lower environmental contamination levels and less likelihood of resistant populations. thus, in this study, the ability of botanical insecticide formulations to inhibit the activity of the liver enzymes serum cholinesterase and mal ... | 2017 | 28001215 |
| a biodegradable device for the controlled release of piper nigrum (piperaceae) standardized extract to control aedes aegypti (diptera, culicidae) larvae. | the significant increase in dengue, zika, and chikungunya and the resistance of the aedes aegypti mosquito to major insecticides emphasize the importance of studying alternatives to control this vector. the aim of this study was to develop a controlled-release device containing piper nigrum extract and to study its larvicidal activity against aedes aegypti. | 2017 | 28001214 |
| bombyx mori and aedes aegypti form multi-functional immune complexes that integrate pattern recognition, melanization, coagulants, and hemocyte recruitment. | the innate immune system of insects responds to wounding and pathogens by mobilizing multiple pathways that provide both systemic and localized protection. key localized responses in hemolymph include melanization, coagulation, and hemocyte encapsulation, which synergistically seal wounds and envelop and destroy pathogens. to be effective, these pathways require a targeted deposition of their components to provide protection without compromising the host. extensive research has identified a larg ... | 2017 | 28199361 |
| meteorological variables and mosquito monitoring are good predictors for infestation trends of aedes aegypti, the vector of dengue, chikungunya and zika. | aedes aegypti is an important vector for arboviroses and widely distributed throughout the world. climatic factors can influence vector population dynamics and, consequently, disease transmission. the aim of this study was to characterize the temporal dynamics of an ae. aegypti population and dengue cases and to investigate the relationship between meteorological variables and mosquito infestation. | 2017 | 28193291 |
| prevalence of dengue and diversity of cultivable bacteria in vector aedes aegypti (l.) from two dengue endemic districts, kanchanpur and parsa of nepal. | dengue fever, an endemic arboviral disease, represents one of the major public health concerns in nepal. it is transmitted by bites of infected aedes aegypti and aedes albopictus, the former being primary vector. the bacterial community plays a significant role in biology of mosquitoes; however, the bacterial communities of primary vector a. aegypti remain unstudied in nepal. the study was designed to determine the rate of dengue seropositivity and to explore the bacterial diversity of a. aegypt ... | 2017 | 28193261 |
| leptolegnia chapmanii (straminipila: peronosporomycetes) as a future biorational tool for the control of aedes aegypti (l.). | the aim of the present review is to summarize the current knowledge about leptolegnia chapmanii as a pathogen of mosquito larvae. to this end, we present data on its identification, distribution, host range and effects on non-target organisms, effects of environmental factors, in vitro growth, release and persistence in anthropic environments, and effect combined with other insecticides. the data presented allow confirming its potential as a biocontrol agent. | 2017 | 28188766 |
| zika and chikungunya: mosquito-borne viruses in a changing world. | the reemergence and growing burden of mosquito-borne virus infections have incited public fear and growing research efforts to understand the mechanisms of infection-associated health outcomes and to provide better approaches for mosquito vector control. while efforts to develop therapeutics, vaccines, and novel genetic mosquito-control technologies are underway, many important underlying ecological questions remain that could significantly enhance our understanding and ability to predict and pr ... | 2017 | 28187236 |
| aedes aegypti larvicidal sesquiterpene alkaloids from maytenus oblongata. | four new sesquiterpene alkaloids (1-4) with a β-dihydroagrofuran skeleton and a new triterpenoid (5) were isolated from an ethyl acetate extract of maytenus oblongata stems. their structures were elucidated using 1d and 2d nmr spectroscopy as well as ms and ecd experiments. the m. oblongata stem etoac extract and the pure compounds isolated were tested for larvicidal activity against aedes aegypti under laboratory conditions, and compounds 2 and 3 were found to be active. | 2017 | 28186749 |
| single molecule rna sequencing uncovers trans-splicing and improves annotations in anopheles stephensi. | single molecule real-time (smrt) sequencing has recently been used to obtain full-length cdna sequences that improve genome annotation and reveal rna isoforms. here, we used one such method called isoform sequencing from pacific biosciences (pacbio) to sequence a cdna library from the asian malaria mosquito anopheles stephensi. more than 600 000 full-length cdnas, referred to as reads of insert, were identified. owing to the inherently high error rate of pacbio sequencing, we tested different ap ... | 2017 | 28181326 |
| xanthine dehydrogenase-1 silencing in aedes aegypti mosquitoes promotes a blood feeding-induced adulticidal activity. | aedesaegypti has 2 genes encoding xanthine dehydrogenase (xdh). we analyzed xdh1 and xdh2 gene expression by real-time quantitative pcr in tissues from sugar- and blood-fed females. differential xdh1 and xdh2 gene expression was observed in tissues dissected throughout a time course. we next exposed females to blood meals supplemented with allopurinol, a well-characterized xdh inhibitor. we also tested the effects of injecting double-stranded rna (dsrna) against xdh1, xdh2, or both. disruption o ... | 2017 | 28179423 |
| mosquitocidal activity and mode of action of the isoxazoline fluralaner. | mosquitoes, such as aedes aegypti and anopheles gambiae, are important vectors of human diseases. fluralaner, a recently introduced parasiticide, was evaluated as a mosquitocide in this study. on ae. aegypti and an. gambiae fourth-instar larvae, fluralaner had 24-h lc50 (lethal concentration for 50% mortality) values of 1.8 ppb and 0.4 ppb, respectively. following topical application to adult ae. aegypti, fluralaner toxicity reached a plateau in about 3 days, with 1- and 3-day ld50 (lethal dose ... | 2017 | 28178191 |
| sweet waste extract uptake by a mosquito vector: survival, biting, fecundity responses, and potential epidemiological significance. | in nature, adult mosquitoes typically utilize nectar as their main energy source, but they can switch to other as yet unidentified sugary fluids. contemporary lifestyles, with their associated unwillingness to consume leftovers and improper disposal of waste, have resulted in the disposal of huge amounts of waste into the environment. such refuse often contains unfinished food items, many of which contain sugar and some of which can collect water from rain and generate juices. despite evidence t ... | 2017 | 28174057 |
| reducing biting rates of aedes aegypti with metofluthrin: investigations in time and space. | indoor residual spraying is key to dengue control in cairns and other parts of northern queensland, australia, where aedes aegypti is prevalent, but the strategy faces challenges with regards to slow application time and, therefore, community coverage. a faster potential improvement might be the use of polyethylene netting impregnated with the volatile pyrethroid metofluthrin (sumione™). this formulation was assessed in rooms in three houses in cairns, australia. one emanator was placed in each ... | 2017 | 28173824 |
| modeling the flux of metabolites in the juvenile hormone biosynthesis pathway using generalized additive models and ordinary differential equations. | juvenile hormone (jh) regulates development and reproductive maturation in insects. the corpora allata (ca) from female adult mosquitoes synthesize fluctuating levels of jh, which have been linked to the ovarian development and are influenced by nutritional signals. the rate of jh biosynthesis is controlled by the rate of flux of isoprenoids in the pathway, which is the outcome of a complex interplay of changes in precursor pools and enzyme levels. a comprehensive study of the changes in enzymat ... | 2017 | 28158248 |
| evaluation of larvicidal activity and ecotoxicity of linalool, methyl cinnamate and methyl cinnamate/linalool in combination against aedes aegypti. | the frequent use of synthetic pesticides to control aedes aegypti population can lead to environmental and/or human contamination and the emergence of resistant insects. linalool and methyl cinnamate are presented as an alternative to the synthetic pesticides, since they can exhibit larvicidal, repellent and/or insecticidal activity and are considered safe for use. the aim of this study was to evaluate the larvicidal activity of methyl cinnamate, linalool and methyl cinnamate/linalool in combina ... | 2017 | 28152405 |
| collective behavior quantification on human odor effects against female aedes aegypti mosquitoes-open source development. | classifying and quantifying mosquito activity includes a plethora of categories, ranging from measuring flight speeds, repellency, feeding rates, and specific behaviors such as home entry, swooping and resting, among others. entomologists have been progressing more toward using machine vision for efficiency for this endeavor. digital methods have been used to study the behavior of insects in labs, for instance via three-dimensional tracking with specialized cameras to observe the reaction of mos ... | 2017 | 28152031 |
| molecular characterization and expression analysis of p38 mapk gene and protein in aquatic midge, chironomus riparius (diptera: chironomidae), exposed to environmental contaminants. | p38 mitogen-activated protein kinase (mapk), an important signaling protein involved in various cellular processes, including stress responses, has been well characterized in model organisms. p38 has been identified in a number of insects, including the genus drosophila; however, its homologue in chironomus riparius has not yet been identified. in this study, we identified and characterized p38 mapk (crp38) gene in c. riparius using a transcriptome database that was previously generated 454 gs-f ... | 2017 | 28144697 |
| toxicological evaluation of essential oil from the leaves of croton argyrophyllus (euphorbiaceae) on aedes aegypti (diptera: culicidae) and mus musculus (rodentia: muridae). | plant-derived essential oils can be used as insecticides for vector control. however, to establish their safety, it is necessary to perform toxicological studies. herein, we evaluated the chemical composition and insecticidal activity of the essential oil from the leaves of croton argyrophyllus on the third- and fourth-instar larvae and adult aedes aegypti (l., 1762). we also evaluated the acute toxicity of the essential oil in adult female mus musculus the lethal concentration 50 (lc50) and 90 ... | 2017 | 28132021 |
| [aedes aegypti and aedes albopictus in bromeliads grown in the bauru municipal botanical gardens, são paulo, brazil]. | the aim of this study was to observe the occurrence of mosquito larvae, especially aedes aegypti and aedes albopictus, in the tanks and axillae of bromeliads at the bauru municipal botanical gardens, são paulo, brazil, highlighting the epidemiological implications for the use of these plants. the majority of the larvae belonged to mosquitos from genus culex, with only occasional findings of a. aegypti and a. albopictus. the use of screens for protection of the plants, exposure to sunlight, and l ... | 2017 | 28125119 |
| malpighian tubules as novel targets for mosquito control. | the malpighian tubules and hindgut are the renal excretory tissues of mosquitoes; they are essential to maintaining hemolymph water and solute homeostasis. moreover, they make important contributions to detoxifying metabolic wastes and xenobiotics in the hemolymph. we have focused on elucidating the molecular mechanisms of malpighian tubule function in adult female mosquitoes and developing chemical tools as prototypes for next-generation mosquitocides that would act via a novel mechanism of act ... | 2017 | 28125032 |
| new pesticidal diterpenoids from vellozia gigantea (velloziaceae), an endemic neotropical plant living in the endangered brazilian biome rupestrian grasslands. | vellozia gigantea is a rare, ancient, and endemic neotropical plant present in the brazilian rupestrian grasslands. the dichloromethane extract of v. gigantea adventitious roots was phytotoxic against lactuca sativa, agrostis stolonifera, and lemna paucicostata, and showed larvicidal activity against aedes aegypti. phytotoxicity bioassay-directed fractionation of the extract revealed one new isopimaradiene, 8(9),15-isopimaradien-1,3,7,11-tetraone, and three new cleistanthane diterpenoids, 7-oxo- ... | 2017 | 28117710 |
| histone-derived pirna biogenesis depends on the ping-pong partners piwi5 and ago3 in aedes aegypti. | the pirna pathway is of key importance in controlling transposable elements in most animal species. in the vector mosquito aedes aegypti, the presence of eight piwi proteins and the accumulation of viral pirnas upon arbovirus infection suggest additional functions of the pirna pathway beyond genome defense. to better understand the regulatory potential of this pathway, we analyzed in detail host-derived pirnas in a. aegypti aag2 cells. we show that a large repertoire of protein-coding genes and ... | 2017 | 28115625 |
| pyrethroid resistance in malaysian populations of dengue vector aedes aegypti is mediated by cyp9 family of cytochrome p450 genes. | dengue control and prevention rely heavily on insecticide-based interventions. however, insecticide resistance in the dengue vector aedes aegypti, threatens the continued effectiveness of these tools. the molecular basis of the resistance remains uncharacterised in many endemic countries including malaysia, preventing the design of evidence-based resistance management. here, we investigated the underlying molecular basis of multiple insecticide resistance in ae. aegypti populations across malays ... | 2017 | 28114328 |
| first report of stegomyia aegypti (= aedes aegypti) in mexico city, mexico. | stegomyia aegypti (= aedes aegypti) (diptera: culicidae) is a species of mosquito that is currently widespread in mexico. historically, the mosquito has been distributed across most tropical and subtropical areas lower than 1700 m a.s.l. currently, populations that are found at higher altitudes in regions with cold and dry climates suggest that these conditions do not limit the colonization and population growth of s. aegypti. during a survey of mosquitoes in september 2015, larvae of s. aegypti ... | 2017 | 28106260 |
| proteomics informed by transcriptomics for characterising active transposable elements and genome annotation in aedes aegypti. | aedes aegypti is a vector for the (re-)emerging human pathogens dengue, chikungunya, yellow fever and zika viruses. almost half of the ae. aegypti genome is comprised of transposable elements (tes). transposons have been linked to diverse cellular processes, including the establishment of viral persistence in insects, an essential step in the transmission of vector-borne viruses. however, up until now it has not been possible to study the overall proteome derived from an organism's mobile geneti ... | 2017 | 28103802 |
| mitigating diseases transmitted by aedes mosquitoes: a cluster-randomised trial of permethrin-impregnated school uniforms. | viral diseases transmitted via aedes mosquitoes are on the rise, such as zika, dengue, and chikungunya. novel tools to mitigate aedes mosquitoes-transmitted diseases are urgently needed. we tested whether commercially insecticide-impregnated school uniforms can reduce dengue incidence in school children. | 2017 | 28103255 |
| ecotoxicological effects of larvicide used in the control of aedes aegypti on nontarget organisms: redefining the use of pyriproxyfen. | the continued widespread use of larvicides in aedes aegypti control programs is still a necessary strategy, since there are no apparent efficient vaccines against arboviruses. however, chemical approaches may affect nontarget organisms and produce detrimental effects to environmental health. therefore, the aim of this study was to conduct toxicity testing for pyriproxyfen at different concentrations using daphnia magna and artemia salina as model organisms to evaluate the ecotoxicological parame ... | 2017 | 28095184 |
| excito-repellency of citrus hystrix dc leaf and peel essential oils against aedes aegypti and anopheles minimus (diptera: culicidae), vectors of human pathogens. | the essential oils of kaffir lime (citrus hystrix dc.) at four different concentrations (0.5, 1.0, 2.5, and 5.0% v/v) were studied for their repellency, excitation, and knockdown properties against laboratory strains of aedes aegypti (l.) and anopheles minimus theobald using an excito-repellency test system. both contact and noncontact escape responses to leaf- and peel-derived kaffir lime oils were observed. comparing unadjusted escape responses for an. minimus, leaf oil had strong combined irr ... | 2017 | 28082646 |
| toxicity and larvicidal activity of podophyllum-based lignans against aedes aegypti (diptera: culicidae). | aedes aegypti l. (diptera: culicidae) is a mosquito species that has adapted to urban environments and is the main vector of dengue viruses. because of the increasing incidence of dengue, a more environmentally acceptable insecticide needs to be found. natural products have been and continue to be an important source of leading compounds that can be modified in order to develop new drugs. the lignan family of natural products includes compounds with a diverse spectrum of biological activity. pod ... | 2017 | 28082643 |
| evaluation of clove oil, icaridin, and transfluthrin for spatial repellent effects in three tests systems against the aedes aegypti (diptera: culicidae). | one essential oil (clove oil), one skin repellent (icaridin), and one insecticide (transfluthrin) were tested for spatial repellent effects against non-blood-fed female aedes aegypti (l.) mosquitoes. the compounds were tested in acetone dilution series using a y-olfactometer, a double cage system, and a double room system. all compounds exhibited spatial repellent effects at certain concentrations. clove oil required relative high dosages to cause high effects (y-olfactometer 6 mg, double cage 6 ... | 2017 | 28082642 |
| egg hatching and survival of immature stages of aedes aegypti (diptera: culicidae) under natural temperature conditions during the cold season in buenos aires, argentina. | in temperate regions, the seasonal dynamics of aedes aegypti (l.) (diptera: culicidae) is mainly influenced by temperature. it is assumed that, during the winter season, the population remains as eggs and that the development and population growth of surviving eggs begin during the following spring. the aim of the current study was to assess egg hatching of ae. aegypti during the winter in buenos aires city (argentina), and analyze the survival of immature stages. the experiments consisted of im ... | 2017 | 28082637 |
| learning in mosquito larvae (aedes aegypti): habituation to a visual danger signal. | in spite of the mosquito aedes aegypti being a vector of several infectious diseases, a limited number of studies has been undertaken on learning in this species. moreover, larval stages have been neglected as model organisms, although they are active, aquatic and perform stereotyped behavioural responses, e.g. the escape response when disturbed. to study the learning abilities of mosquito larvae, we focused on habituation, a form of non-associative learning widely studied in vertebrates and inv ... | 2017 | 28077263 |
| rna interference for mosquito and mosquito-borne disease control. | rna interference (rnai) is a powerful tool to silence endogenous mosquito and mosquito-borne pathogen genes in vivo. as the number of studies utilizing rnai in basic research grows, so too does the arsenal of physiological targets that can be developed into products that interrupt mosquito life cycles and behaviors and, thereby, relieve the burden of mosquitoes on human health and well-being. as this technology becomes more viable for use in beneficial and pest insect management in agricultural ... | 2017 | 28067782 |
| the antiviral rnai response in vector and non-vector cells against orthobunyaviruses. | vector arthropods control arbovirus replication and spread through antiviral innate immune responses including rna interference (rnai) pathways. arbovirus infections have been shown to induce the exogenous small interfering rna (sirna) and piwi-interacting rna (pirna) pathways, but direct antiviral activity by these host responses in mosquito cells has only been demonstrated against a limited number of positive-strand rna arboviruses. for bunyaviruses in general, the relative contribution of sma ... | 2017 | 28060823 |
| wingbeat frequency-sweep and visual stimuli for trapping male aedes aegypti (diptera: culicidae). | combinations of female wingbeat acoustic cues and visual cues were evaluated to determine their potential for use in male aedes aegypti (l.) traps in peridomestic environments. a modified centers for disease control (cdc) light trap using a 350-500 hz frequency-sweep broadcast from a speaker as an acoustic stimulus, combined with a black poster-board half-cylinder behind the trap as a visual stimulus, captured a significantly greater proportion of males in a laboratory arena during daylight than ... | 2017 | 28399312 |
| effect of temperature thresholds on modeled aedes aegypti (diptera: culicidae) population dynamics. | dynamic simulation models provide vector abundance estimates using only meteorological data. however, model outcomes may heavily depend on the assumptions used to parameterize them. we conducted a sensitivity analysis for a model of aedes aegypti (l.) abundance using weather data from two locations where this vector is established, la margarita, puerto rico and tucson, arizona. we tested the effect of simplifying temperature-dependent development and mortality rates and of changing development a ... | 2017 | 28399306 |
| factors of concern regarding zika and other aedes aegypti-transmitted viruses in the united states. | the recent explosive outbreaks of zika and chikungunya throughout the americas has raised concerns about the threats that these and similar diseases may pose to the united states (u.s.). the commonly accepted association between tropical climates and the endemicity of these diseases has led to concerns about the possibility of their redistribution due to climate change and transmission arising from cases imported from endemic regions initiating outbreaks in the united states. while such possibil ... | 2017 | 28399294 |
| repellent and larvicidal activity of the essential oil from eucalyptus nitens against aedes aegypti and aedes albopictus (diptera: culicidae). | dengue, chikungunya, and yellow fever are important vector-borne diseases transmitted by female mosquitoes when they feed on humans. the use of repellents based on natural products is an alternative for personal protection against these diseases. application of chemicals with larvicidal activity is another strategy for controlling the mosquito population. the repellent and larvicidal activities of the essential oil from eucalyptus nitens were tested against aedes aegypti and aedes albopictus, th ... | 2017 | 28399283 |
| transcriptional profile for detoxification enzymes aeaggt1 and aaeggt2 from aedes aegypti (diptera: culicidae) in response to larvicides. | aedes aegypti (l.) is the vector responsible for transmitting dengue, chikungunya, yellow fever, and zika viruses, as well as other pathogens. microbial larvicides based on bacillus thuringiensis berliner israelensis (bti) and saccharopolyspora spinosa mertz and yao, such as vectobac 12as and natular 2ec, have been shown to be effective in reducing larval populations of ae. aegypti. we examined the gene expression of two detoxification enzymes, glucosyl and glucuronosyl transferases (aaeggt1 and ... | 2017 | 28399278 |
| detection and establishment of aedes aegypti and aedes albopictus (diptera: culicidae) mosquitoes in california, 2011-2015. | in 2011, a thriving population of aedes albopictus (skuse), the asian tiger mosquito, was discovered within three cities in los angeles county over an estimated 52-km2 urban area. two years later in 2013, aedes aegypti (l.), the yellow fever mosquito, was detected within several urban areas of madera, fresno, and san mateo counties. state and local vector control agencies responded with an aggressive effort to eradicate or interrupt the spread of these two invasive mosquitoes; however, known pop ... | 2017 | 28399270 |
| ecological niche model for predicting distribution of disease-vector mosquitoes in yucatán state, méxico. | the majority of the yucatán state, méxico, presents subtropical climate that is suitable for many species of mosquitoes that are known to be vectors of diseases, including those from the genera aedes and culex. the objective of this study is to identify the geographic distribution of five species from these two genera and estimate the human population at risk of coming in contact with them. we compiled distributional data for aedes aegypti (l.), aedes (howardina) cozumelensis (diaz najera), cule ... | 2017 | 28399263 |
| emergent and reemergent arboviruses in south america and the caribbean: why so many and why now? | several arboviruses have emerged and/or reemerged in the new world in the past decades. zika and chikungunya viruses, formerly restricted to africa and perhaps asia, invaded the continent, causing great concern. dengue virus outbreaks have continued to occur in almost all countries, with millions of cases per year. west nile virus rapidly invaded north america, and now cases have been found in central and south america. other arboviruses, such as mayaro and eastern equine encephalitis viruses, h ... | 2017 | 28399216 |
| eleutherococcus senticosus (araliaceae) leaf morpho-anatomy, essential oil composition, and its biological activity against aedes aegypti (diptera: culicidae). | the roots of eleutherococcus senticosus (rupr. & maxim.) maxim., a well-known medicinal plant from eastern asia, are used worldwide for their known beneficial medicinal properties. recently, the leaves have been used as an alternative to the roots. the present study was aimed at exploring the leaf essential oil as a potential source of compounds for mosquito management. gas chromatography/mass spectrometry analysis of the leaf essential oil revealed 87 compounds, constituting 95.2% of the oil. α ... | 2017 | 28399215 |
| a video-tracking analysis-based behavioral assay for larvae of anopheles pseudopunctipennis and aedes aegypti (diptera: culicidae). | aedes aegypti (l.) is the primary vector of dengue, yellow fever, zika, and chikungunya viruses, whereas anopheles pseudopunctipennis (theobald) is the principal vector for malaria in latin america. the larval stage of these mosquitoes occurs in very different development habitats, and the study of their respective behaviors could give us valuable information to improve larval control. the aim of this study was to set up a bioassay to study basic larval behaviors using a video-tracking software. ... | 2017 | 28399206 |
| differential pathogenicity of metarhizium blastospores and conidia against larvae of three mosquito species. | biorational insecticides are being increasingly used in integrated pest management programs. in laboratory bioassays, the pathogenicity of blastospores and conidia of the entomopathogenic fungus metarhizium brunneum arsef 4556 was evaluated against larvae of three mosquito species. three propagule concentrations (1 × 106, 1 × 107, and 1 × 108 spores ml - 1) were used in the bioassays. results showed that aedes aegypti had lower survival rates when exposed to blastospores than when exposed to con ... | 2017 | 28399202 |
| epidemiological characteristics of dengue disease in latin america and in the caribbean: a systematic review of the literature. | dengue, an important mosquito-borne virus transmitted mainly by aedes aegypti, is a major public health issue in latin america and the caribbean. national epidemiological surveillance systems, usually based on passive detection of symptomatic cases, while underestimating the true burden of dengue disease, can provide valuable insight into disease trends and excess reporting and potential outbreaks. we carried out a systematic review of the literature to characterize the recent epidemiology of de ... | 2017 | 28392806 |
| fabrication of highly effective mosquito nanolarvicides using an asian plant of ethno-pharmacological interest, priyangu (aglaia elaeagnoidea): toxicity on non-target mosquito natural enemies. | mosquitoes threaten the lives of humans, livestock, pets and wildlife around the globe, due to their ability to vector devastating diseases. aglaia elaeagnoidea, commonly known as priyangu, is widely employed in asian traditional medicine and pest control. medicinal activities include anti-inflammatory, analgesic, anticancer, and anesthetic actions. flavaglines, six cyclopenta[b]benzofurans, a cyclopenta[bc]benzopyran, a benzo[b]oxepine, and an aromatic butyrolactone showed antifungal properties ... | 2017 | 28390026 |
| insecticidal and repellent activity of several plant-derived essential oils against aedes aegypti. | we examined the pupicidal, adulticidal, repellent, and oviposition-deterrent activities of essential oils (eos) from lippia alba, l. origanoides, eucalyptus citriodora, cymbopogon citratus, cymbopogon flexuosus, citrus sinensis , cananga odorata , swinglea glutinosa, and tagetes lucida plants against aedes aegypti under laboratory conditions. pupicidal and adulticidal activities were assessed at exploratory concentrations of 250, 310, and 390 parts per million (ppm); and 30, 300, and 1,000 ppm, ... | 2017 | 28388322 |
| in the hunt for genomic markers of metabolic resistance to pyrethroids in the mosquito aedes aegypti: an integrated next-generation sequencing approach. | the capacity of aedes mosquitoes to resist chemical insecticides threatens the control of major arbovirus diseases worldwide. until alternative control tools are widely deployed, monitoring insecticide resistance levels and identifying resistance mechanisms in field mosquito populations is crucial for implementing appropriate management strategies. metabolic resistance to pyrethroids is common in aedes aegypti but the monitoring of the dynamics of resistant alleles is impeded by the lack of robu ... | 2017 | 28379969 |
| effects of socio-demographic characteristics and household water management on aedes aegypti production in suburban and rural villages in laos and thailand. | dengue fever is a mosquito-borne disease accounting for 50-100 million annual cases globally. laos and thailand are countries in south-east asia where the disease is endemic in both urban and rural areas. household water storage containers, which are favourable breeding sites for dengue mosquitoes, are common in these areas, due to intermittent or limited access to water supply. this study assessed the effect of household water management and socio-demographic risk factors on aedes aegypti infes ... | 2017 | 28376893 |
| new insights into the in vitro development and virulence of culicinomyces spp. as fungal pathogens of aedes aegypti. | culicinomyces spp. (hypocreales: cordycipitaceae) are facultative fungal pathogens affecting the larval stages from a range of mosquito species and are especially notable in their ability to infect hosts through the digestive tract after conidial ingestion. while culicinomyces spp. were studied mainly in the 1980s, little is yet known about inter- and intraspecific variability of the in vitro development of these fungi at different temperatures, and nothing is known about the impact of serial ho ... | 2017 | 28372997 |
| evolutionary origin and function of nox4-art, an arthropod specific nadph oxidase. | nadph oxidases (nox) are ros producing enzymes that perform essential roles in cell physiology, including cell signaling and antimicrobial defense. this gene family is present in most eukaryotes, suggesting a common ancestor. to date, only a limited number of phylogenetic studies of metazoan noxes have been performed, with few arthropod genes. in arthropods, only nox5 and duox genes have been found and a gene called noxm was found in mosquitoes but its origin and function has not been examined. ... | 2017 | 28356077 |
| larvicidal activity of ottonia anisum metabolites against aedes aegypti: a potential natural alternative source for mosquito vector control in brazil. | aedes aegypti mosquito is the principal vector of the viruses responsible for urban yellow fever, dengue, dengue haemorrhagic fever, as well as zika and chikungunya in brazil. the present study was aimed to investigate the insecticidal potential of the extract and fractions of ottonia anisum, along with special metabolites isolated from it, as natural alternatives against larvae (l3) of ae. aegypti, vector of potentially deadly tropical infections in brazil. | 2017 | 28352047 |
| combined target site (kdr) mutations play a primary role in highly pyrethroid resistant phenotypes of aedes aegypti from saudi arabia. | pyrethroid resistance is a threat to effective vector control of aedes aegypti, the vector of dengue, zika and other arboviruses, but there are many major knowledge gaps on the mechanisms of resistance. in jeddah and makkah, the principal dengue-endemic areas of saudi arabia, pyrethroids are used widely for ae. aegypti control but information about resistance remains sparse, and the underlying genetic basis is unknown. findings from an ongoing study in this internationally significant area are r ... | 2017 | 28347352 |
| first report of the phe1534cys kdr mutation in natural populations of aedes albopictus from brazil. | knockdown resistance (kdr), caused by alterations in the voltage-gated sodium channel (nav), is one of the mechanisms responsible for pyrethroid (py) resistance. in the asian tiger mosquito, aedes albopictus, at least four different mutations were described in the iiis6 nav segment in populations from asia, north america and europe. in contrast, in aedes aegypti at least 12 non-synonymous mutations have been reported at nine different codons, mostly in the iis6 and iiis6 nav segments. the phe153 ... | 2017 | 28347326 |
| the toxicity of angiotensin converting enzyme inhibitors to larvae of the disease vectors aedes aegypti and anopheles gambiae. | the control of mosquitoes is threatened by the appearance of insecticide resistance and therefore new control chemicals are urgently required. here we show that inhibitors of mosquito peptidyl dipeptidase, a peptidase related to mammalian angiotensin-converting enzyme (ace), are insecticidal to larvae of the mosquitoes, aedes aegypti and anopheles gambiae. ace inhibitors (captopril, fosinopril and fosinoprilat) and two peptides (trypsin-modulating oostatic factor/tmof and a bradykinin-potentiati ... | 2017 | 28345667 |
| novel synthesis of gold nanoparticles using artemisia vulgaris l. leaf extract and their efficacy of larvicidal activity against dengue fever vector aedes aegypti l. | the aedes aegypti l. mosquito transmits dengue and yellow fever, which cause millions of death every year. dengue is a mosquito-borne viral disease that has rapidly spread worldwide particularly in countries with tropical and subtropical climates areas. the present study denotes a simple and eco-friendly biosynthesis of gold nanoparticles using artemisia vulgaris l. leaf extract as reducing agent. the synthesized gold nanoparticles were characterized by uv-visible spectroscopy, x-ray diffraction ... | 2017 | 28341392 |
| rapid surveillance for vector presence (rsvp): development of a novel system for detecting aedes aegypti and aedes albopictus. | the globally important zika, dengue and chikungunya viruses are primarily transmitted by the invasive mosquitoes, aedes aegypti and aedes albopictus. in australia, there is an increasing risk that these species may invade highly urbanized regions and trigger outbreaks. we describe the development of a rapid surveillance for vector presence (rsvp) system to expedite presence- absence surveys for both species. | 2017 | 28339458 |
| de novo assembly of the aedes aegypti genome using hi-c yields chromosome-length scaffolds. | the zika outbreak, spread by the aedes aegypti mosquito, highlights the need to create high-quality assemblies of large genomes in a rapid and cost-effective way. here we combine hi-c data with existing draft assemblies to generate chromosome-length scaffolds. we validate this method by assembling a human genome, de novo, from short reads alone (67× coverage). we then combine our method with draft sequences to create genome assemblies of the mosquito disease vectors aeaegypti and culex quinquefa ... | 2017 | 28336562 |
| functional and nonfunctional forms of cquior91, an odorant selectivity subunit of culex quinquefasciatus. | in culex quinquefasciatus, cquior91 is the ortholog of 2 larvae-specific odorant receptors (ors) from anopheles gambiae (agam\or40, previously shown to respond to several odorant ligands including the broad-spectrum repellent n,n-diethyl-3-methylbenzamide, deet) and aedes aegypti (aaeg\or40). when we cloned full-length cquior91 from a culex quinquefasciatus larval head rna sample, we found 2 alleles of this or, differing at 9 residues. functional analysis using the xenopus oocyte expression syst ... | 2017 | 28334229 |
| screening of wolbachia endosymbiont infection in aedes aegypti mosquitoes using attenuated total reflection mid-infrared spectroscopy. | dengue fever is the most common mosquito transmitted viral infection afflicting humans, estimated to generate around 390 million infections each year in over 100 countries. the introduction of the endosymbiotic bacterium wolbachia into aedes aegypti mosquitoes has the potential to greatly reduce the public health burden of the disease. this approach requires extensive polymerase chain reaction (pcr) testing of the wolbachia-infection status of mosquitoes in areas where wolbachia-a. aegypti are r ... | 2017 | 28332822 |