Publications

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larval exposure to the bacterial insecticide bti enhances dengue virus susceptibility of adult aedes aegypti mosquitoes.understanding the interactions between pathogens sharing the same host can be complicated for holometabolous animals when larval and adult stages are exposed to distinct pathogens. in medically important insect vectors, the effect of pathogen exposure at the larval stage may influence susceptibility to human pathogens at the adult stage. we addressed this hypothesis in the mosquito aedes aegypti, a major vector of arthropod-borne viruses (arboviruses), such as the dengue virus (denv) and the chi ...201830558130
evolution of maternal and zygotic mrna complements in the early drosophila embryo.the earliest stages of animal development are controlled by maternally deposited mrna transcripts and proteins. once the zygote is able to transcribe its own genome, maternal transcripts are degraded, in a tightly regulated process known as the maternal to zygotic transition (mzt). while this process has been well-studied within model species, we have little knowledge of how the pools of maternal and zygotic transcripts evolve. to characterize the evolutionary dynamics and functional constraints ...201830557299
aedes aegypti galectin competes with cry11aa for binding to alp1 to modulate cry toxicity.the key step for the toxicity of bacillus thuringiensis subsp. israelensis (bti) is the interaction between toxins and putative receptors; thus, many studies focus on identification of new toxin receptors and engineering of toxins with higher affinity/specificity for receptors. in the larvae of aedes aegypti, galectin-14 was one of the genes upregulated by bti treatment. rnai knockdown expression of galectin-14 and feeding recombinant galectin-14-thioredoxin fusion protein significantly affected ...201830556692
evaluation of the leaf essential oil from artemisia vulgaris and its larvicidal and repellent activity against dengue fever vector aedes aegypti-an experimental and molecular docking investigation.aedes aegypti is a mosquito vector that spreads dengue fever and yellow fever worldwide in tropical and subtropical countries. essential oil isolated from artemisia vulgaris is found to have larvicidal and repellent action against this vector. the dried leaves were subjected to hydrodistillation using a clevenger-type apparatus for 4 h. the isolated essential oil was analyzed by using gas chromatography-mass spectrometry, and the major insecticidal compounds were identified as α-humulene (0.72%) ...201830556010
omics approaches to study juvenile hormone synthesis.the juvenile hormones (jhs) are a family of insect acyclic sesquiterpenoids produced by the corpora allata (ca), a pair of endocrine glands connected to the brain. they are involved in the regulation of development, reproduction, behavior, caste determination, diapause, stress response, and numerous polyphenisms. in the post-genomics era, comprehensive analyses using functional 'omics' technologies such as transcriptomics, proteomics and metabolomics have increased our understanding of the activ ...201830551825
genetic variability of the aedes aegypti (diptera: culicidae) mosquito in el salvador, vector of dengue, yellow fever, chikungunya and zika.aedes aegypti is associated with dengue, yellow fever, chikungunya and zika viruses. this vector is widespread in tropical and subtropical areas, and can also occur in temperate areas at higher latitudes. the geographical distribution of ae. aegypti continues to spread due to human activities. this is the first study to examine the population genetic structure of this insect in el salvador, central america.201830547835
susceptible and mcry3a resistant corn rootworm larvae killed by a non-hemolytic bacillus thuringiensis cyt1aa mutant.the western corn rootworm (wcr) diabrotica virgifera virgifera causes substantial damage in corn. genetically modified (gm) plants expressing some bacillus thuringiensis (bt) insecticidal cry proteins efficiently controlled this pest. however, changes in wcr susceptibility to these bt traits have evolved and identification of insecticidal proteins with different modes of action against wcr is necessary. we show here for the first time that cyt1aa from bt exhibits toxicity against wcr besides to ...201830546034
aspergillus terreus (trichocomaceae): a natural, eco-friendly mycoinsecticide for control of malaria, filariasis, dengue vectors and its toxicity assessment against an aquatic model organism artemia nauplii.vector-borne diseases like malaria, filariasis, and dengue are transmitted by mosquitoes and they cause global mortality and morbidity due to an increased resistance against commercial insecticides. the present study was aimed to evaluate the neurobehavioral toxicity, knock-down effect, histopathology, ovicidal, adulticidal, and smoke toxicity effect of aspergillus terreus extract against three mosquito species, namely anopheles stephensi, culex quinquefasciatus, and aedes aegypti (diptera: culi ...201830534070
multispecies transcriptomics data set of brugia malayi, its wolbachia endosymbiont wbm, and aedes aegypti across the b. malayi life cycle.here, we present a comprehensive transcriptomics data set of brugia malayi, its wolbachia endosymbiont wbm, and its vector host. this study samples from 16 stages across the entire b. malayi life cycle, including stage 1 through 4 larvae, adult males and females, embryos, immature microfilariae, and mature microfilariae.201830533772
comparison of landmark- and outline-based geometric morphometrics for discriminating mosquito vectors in ratchaburi province, thailand.it is often challenging to identify mosquito vectors in the field based on morphological features due to their similar morphologies and difficulties in obtaining undamaged samples but is required for their successful control. geometric morphometrics (gm) overcomes this issue by analyzing a suite of traits simultaneously and has the added advantages of being easy to use, low cost, and quick. therefore, this research compared the efficiency and precision of landmark- and outline-based gm technique ...201830533435
dengue outbreaks in hawai'i after wwii - a review of public health response and scientific literature.the four serotypes of dengue virus (denv) cause the most important and common arthropod-borne viral diseases in humans. there have been three major dengue outbreaks in hawai'i since 1946. the most recent and largest outbreak occurred on hawai'i island in 2015-2016. this article reviews the public health response to dengue outbreaks over the period 2001-2016, as well as scientific literature on dengue outbreaks in hawai'i. as summarized in the assessment by the centers for disease control and pre ...201830533283
correction: mosquito pornoscopy: observation and interruption of aedes aegypti copulation to determine female polyandric event and mixed progeny.[this corrects the article doi: 10.1371/journal.pone.0193164.].201830532207
vector competence of biting midges and mosquitoes for shuni virus.shuni virus (shuv) is an orthobunyavirus that belongs to the simbu serogroup. shuv was isolated from diverse species of domesticated animals and wildlife, and is associated with neurological disease, abortions, and congenital malformations. recently, shuv caused outbreaks among ruminants in israel, representing the first incursions outside the african continent. the isolation of shuv from a febrile child in nigeria and seroprevalence among veterinarians in south africa suggests that the virus ma ...201830532189
zika in puerto rico, 2016-2017: ii perspectives on epidemic surveillance and control, health communication, outcomes and lessons.the social reaction to the zika epidemic in puerto rico reached a confrontational climax regarding aerial fumigation with an organophosphate insecticide. the public drama has obscured multiple simultaneous controversies. this and a companion paper, based mostly on print and digital news reports, provide a context and description of the major controversies and examine the outcomes and their lessons for the protection of the public's health. part ii covers the questions on disease surveillance (wh ...201830576574
zika virus in the americas: an environmental health perspective.phylogenetic studies suggest that zikv may have been introduced to brazil, and therefore to the americas, in 2014 during the world spring canoe championship held in the city of rio de janeiro. since then the virus has spread across latin america, caribbean, and north america. it seems clear that aedes aegypti and, to a lesser extent, aedes albopictus are the main vectors of the pathogen. zikv infection symptoms are similar to other flaviviruses such as a dengue infection and therefore can be eas ...201830576572
the pagodas protocol: pediatric assessment group of dengue and aedes saliva protocol to investigate vector-borne determinants of aedes-transmitted arboviral infections in cambodia.mosquito-borne arboviruses, like dengue virus, continue to cause significant global morbidity and mortality, particularly in southeast asia. when the infectious mosquitoes probe into human skin for a blood meal, they deposit saliva containing a myriad of pharmacologically active compounds, some of which alter the immune response and influence host receptivity to infection, and consequently, the establishment of the virus. previous reports have highlighted the complexity of mosquito vector-derive ...201830572920
construction sites in miami-dade county, florida are highly favorable environments for vector mosquitoes.urbanization is increasing globally, and construction sites are an integral part of the urbanization process. it is unknown to what extent construction sites create favorable breeding conditions for mosquitoes. the main objectives of the present study were to identify what species of mosquitoes are present at construction sites and the respective physical features associated with their production. eleven construction sites were cross-sectionally surveyed for the presence of mosquitoes in miami-d ...201830571764
the composition of midgut bacteria in aedes aegypti (diptera: culicidae) that are naturally susceptible or refractory to dengue viruses.the composition, abundance, and diversity of midgut bacteria in mosquitoes can influence pathogen transmission. we used 16s rrna microbiome profiling to survey midgut microbial diversity in pooled samples of laboratory colonized dengue-refractory, cali-mib, and dengue-susceptible, cali-s aedes aegypti (linnaeus). the 16s rrna sequences from the sugar-fed midguts of adult females clustered to 63 amplicon sequence variants (asvs), primarily from proteobacteria, firmicutes, flavobacteria, and actin ...201830508201
optimization of double-stranded rnai intrathoracic injection method in aedes aegypti.rna interference is widely used to analyze gene functions via phenotypic knockdown of target transcripts in mosquitoes, which transmit numerous mosquito-borne diseases. functional analysis of mosquito genes is indispensable to understand and reduce transmission of mosquito-borne diseases in mosquitoes. intrathoracic injection of double-stranded rna (dsrna) remains the simplest and most customizable method in mosquitoes for functional analysis of the genes of interest. however, achieving consiste ...201830505333
risk prediction system for dengue transmission based on high resolution weather data.dengue is the fastest spreading vector-borne viral disease, resulting in an estimated 390 million infections annually. precise prediction of many attributes related to dengue is still a challenge due to the complex dynamics of the disease. important attributes to predict include: the risk of and risk factors for an infection; infection severity; and the timing and magnitude of outbreaks. in this work, we build a model for predicting the risk of dengue transmission using high-resolution weather d ...201830521550
[costs of an aedes aegypti vector control program in municipalities in colombia: a case study in girón and guadalajara de buga, 2016].the aim of this study was to measure the costs of vector-borne disease control programs at the local level in colombia (2016). a cost analysis was performed for this purpose from the policymaker's perspective in the municipalities (counties) of girón and guadalajara de buga, specifically for the aedes aegypti control program, the principal mosquito vector of dengue, zika, and chikungunya. the analysis involved the quantification of all the costs required for each of the prevention and control st ...201830517314
sequential infection of aedes aegypti mosquitoes with chikungunya virus and zika virus enhances early zika virus transmission.in urban settings, chikungunya, zika, and dengue viruses are transmitted by aedes aegypti mosquitoes. since these viruses co-circulate in several regions, coinfection in humans and vectors may occur, and human coinfections have been frequently reported. yet, little is known about the molecular aspects of virus interactions within hosts and how they contribute to arbovirus transmission dynamics. we have previously shown that aedes aegypti exposed to chikungunya and zika viruses in the same blood ...201830513725
a scoping review of published literature on chikungunya virus.chikungunya virus (chikv) has caused several major epidemics globally over the last two decades and is quickly expanding into new areas. although this mosquito-borne disease is self-limiting and is not associated with high mortality, it can lead to severe, chronic and disabling arthritis, thereby posing a heavy burden to healthcare systems. the two main vectors for chikv are aedes aegypti and aedes albopictus (asian tiger mosquito); however, many other mosquito species have been described as com ...201830496207
mosquitoes modulate leg dynamics at takeoff to accommodate surface roughness.insects perform takeoffs from a nearly unquantifiable number of surface permutations and many use their legs to initiate upward movement prior to the onset of wingbeats, including the mosquito. in this study we examine the unprovoked pre-takeoff mechanics of aedes aegypti mosquitoes from two surfaces of contrasting roughness, one with roughness similar to polished glass and the other comparable to the human forearm. using high-speed videography, we find mosquitos exhibit two distinct leg actions ...201830479315
a novel highly divergent strain of cell fusing agent virus (cfav) in mosquitoes from the brazilian amazon region.classical insect-specific flaviviruses (cisfs) have been widely detected in different countries in the last decades. here, we characterize the near full-length genomes of two cisfs detected in mosquitoes collected in the city of macapá, state of amapá, amazon region of brazil. a total of 105 pools of female mosquitos were analyzed by next-generation sequencing (ngs). comparative genomics and phylogenetic analysis identified three strains of cell fusing agent virus (cfav) and two of culex flavivi ...201830477235
void entry by aedes aegypti (diptera: culicidae) mosquitoes is lower than would be expected by a randomized search.insects enter every passible space on the planet. despite our best efforts, flying insects infiltrate slightly open windows in domiciles, automobiles, storage spaces, and more. is this ubiquitous experience a consequence of insect abundance and probability, or are flying insects adept at detecting passageways? there remains a lack of understanding of insect effectiveness in finding passage through the voids and imperfections in physical barriers in response to attractants, a topic particularly c ...201830476211
global spatial assessment of aedes aegypti and culex quinquefasciatus: a scenario of zika virus exposure.zika virus (zikv) is an arbovirus transmitted mainly by aedes aegypti mosquitoes. recent scientific evidence on culex quinquefasciatus has suggested its potential as a vector for zikv, which may change the current risk zones. we aimed to quantify the world population potentially exposed to zikv in a spatially explicit way, considering the primary vector (a. aegypti) and the potential vector (c. quinquefasciatus). our model combined species distribution modelling of mosquito species with spatiall ...201830474578
alternative insecticides for larval control of the dengue vector aedes aegypti in lao pdr: insecticide resistance and semi-field trial study.the mosquito aedes aegypti is the primary vector of several arboviruses, such as dengue, chikungunya and zika, and represents a major public health problem in southeast asia. in laos, where dengue is reemerging, several ae. aegypti populations from the capital vientiane have shown resistance to the organophosphate temephos, a commonly-used larvicide for public health interventions.201830509299
zikv demonstrates minimal pathologic effects and mosquito infectivity in viremic cynomolgus macaques.to evaluate the effects of zikv infection on non-human primates (nhps), as well as to investigate whether these nhps develop sufficient viremia to infect the major urban vector mosquito, aedes aegypti, four cynomolgus macaques (macaca fascicularis) were subcutaneously infected with 5.0 log10 focus-forming units (ffu) of dna clone-derived zikv strain fss13025 (asian lineage, cambodia, 2010). following infection, the animals were sampled (blood, urine, tears, and saliva), underwent daily health mo ...201830469417
recent history of aedes aegypti: vector genomics and epidemiology records.aedes aegypti bears the common name "the yellow fever mosquito," although, today, it is of more concern as the major vector of dengue, chikungunya, and, most recently, zika viruses. in the present article, we review recent work on the population genetics of this mosquito in efforts to reconstruct its recent (approximately 600 years) history and relate these findings to epidemiological records of occurrences of diseases transmitted by this species. the two sources of information are remarkably co ...201830464351
longevity of mass-reared, irradiated and packed male anopheles arabiensis and aedes aegypti under simulated environmental field conditions.to ensure the success of a mosquito control programme that integrates the sterile insect technique (sit), it is highly desirable to release sterile males with a maximal lifespan to increase release effectiveness. understanding sterile male survival under field conditions is thus critical for determining the number of males to be released. our study aimed to investigate the effect of mass rearing, irradiation, chilling, packing and release time on irradiated male mosquito longevity.201830463624
vertical transmission of naturally occurring bunyamwera and insect-specific flavivirus infections in mosquitoes from islands and mainland shores of lakes victoria and baringo in kenya.many arboviruses transmitted by mosquitoes have been implicated as causative agents of both human and animal illnesses in east africa. although epidemics of arboviral emerging infectious diseases have risen in frequency in recent years, the extent to which mosquitoes maintain pathogens in circulation during inter-epidemic periods is still poorly understood. this study aimed to investigate whether arboviruses may be maintained by vertical transmission via immature life stages of different mosquit ...201830452443
cyp-mediated permethrin resistance in aedes aegypti and evidence for trans-regulation.aedes aegypti poses a serious risk to human health due to its wide global distribution, high vector competence for several arboviruses, frequent human biting, and ability to thrive in urban environments. pyrethroid insecticides remain the primary means of controlling adult a. aegypti populations during disease outbreaks. as a result of decades of use, pyrethroid resistance is a global problem. cytochrome p450 monooxygenase (cyp)-mediated detoxification is one of the primary mechanisms of pyrethr ...201830452436
air versus water temperature of aquatic habitats in delhi: implications for transmission dynamics of aedes aegypti.the present study was planned to characterize the microclimate experienced by aedes larvae in different breeding habitats by determining the temperature variations in water kept in containers during different months under natural conditions. the study was conducted in three municipal zones of delhi. in each site, four types of container material (plastic, cement, iron and ceramic) were chosen for recording the water temperature in the containers. daily air and water temperatures (mean, maximum a ...201830451475
operational satellite-based temporal modelling of aedes population in argentina.aedes aegypti is a vector for chikungunya, dengue and zika viruses in latin america and is therefore a large public health problem for the region. for this reason, several inter-institutional and multidisciplinary efforts have been made to support vector control actions through the use of geospatial technologies. this study presents the development of an operational system for the application of free access to remotely sensed products capable of assessing the oviposition activity of ae. aegypti ...201830451465
innate immune response of primary human keratinocytes to west nile virus infection and its modulation by mosquito saliva.west nile virus (wnv) is a flavivirus involved in many human infections worldwide. this arthropod-borne virus is directly co-inoculated with mosquito saliva through the epidermis and the dermis during blood meal. wnv starts replicating in the skin before migrating to the draining lymph node, leading to widespread viremia and in some cases to neurological symptoms. skin is a complex organ composed of different cell types that together perform essential functions such as pathogen sensing, barrier ...201830450338
pharmacological inhibition of inward rectifier potassium channels induces lethality in larval aedes aegypti.the inward rectifier potassium (kir) channels play key roles in the physiology of mosquitoes and other insects. our group, among others, previously demonstrated that small molecule inhibitors of kir channels are promising lead molecules for developing new insecticides to control adult female mosquitoes. however, the potential use of kir channel inhibitors as larvicidal agents is unknown. here we tested the hypothesis that pharmacological inhibition of kir channels in the larvae of aedes aegypti, ...201830445675
a comprehensive review of the manifestations and pathogenesis of zika virus in neonates and adults.the zika virus (zikv) has been slowly becoming an epidemic in different parts of the world. since its discovery in 1947, there have been numerous outbreaks affecting many different populations. currently, there is an ongoing threat of zikv in latin america and the united states of america. zikv is mainly spread by the aedesaegypti mosquito and causes non-specific symptoms such as fever, myalgia, and generalized weakness. in addition to these symptoms, it has been noted the zikv is capable of cau ...201830443460
competition among aedes aegypti larvae.adult aedes aegypti mosquitoes are important vectors of human disease. the size of the adult female affects her success, fitness, and ability to transmit diseases. the size of the adults is determined during the aquatic larval stage. competition among larvae for food influences the size of the pupa and thus the adult. in these experiments, the food level (mg/larva) and the density (larvae/vial) both affect intraspecific competition, which shows up as the interaction of the two factors. furthermo ...201830439951
improved reference genome of aedes aegypti informs arbovirus vector control.female aedes aegypti mosquitoes infect more than 400 million people each year with dangerous viral pathogens including dengue, yellow fever, zika and chikungunya. progress in understanding the biology of mosquitoes and developing the tools to fight them has been slowed by the lack of a high-quality genome assembly. here we combine diverse technologies to produce the markedly improved, fully re-annotated aaegl5 genome assembly, and demonstrate how it accelerates mosquito science. we anchored phys ...201830429615
restriction of zika virus infection and transmission in aedes aegypti mediated by an insect-specific flavivirus.previous studies demonstrated an insect-specific flavivirus, nhumirim virus (nhuv), can suppress growth of west nile virus (wnv) and decrease transmission rates in nhuv/wnv co-inoculated culex quinquefasciatus. to assess whether nhuv might interfere with transmission of other medically important flaviviruses, the ability of nhuv to suppress viral growth of zika virus (zikv) and dengue-2 virus (denv-2) was assessed in aedes albopictus cells. significant reductions in zikv (100,000-fold) and denv- ...201830429457
effectiveness of a field trap barrier system for controlling aedes albopictus: a "removal trapping" strategy.aedes aegypti and aedes albopictus are the main vectors for the transmission of several viral pathogens, in particular, dengue, zika and chikungunya. in the absence of vaccines and treatment, control of aedes mosquitoes is the only means of keeping these diseases in check. aedes control is difficult, and it is, therefore, necessary to evaluate the efficacy of novel control methods, particularly those targeting adult and exophilic ae. albopictus populations.201829463300
host plant forensics and olfactory-based detection in afro-tropical mosquito disease vectors.the global spread of vector-borne diseases remains a worrying public health threat, raising the need for development of new combat strategies for vector control. knowledge of vector ecology can be exploited in this regard, including plant feeding; a critical resource that mosquitoes of both sexes rely on for survival and other metabolic processes. however, the identity of plant species mosquitoes feed on in nature remains largely unknown. by testing the hypothesis about selectivity in plant feed ...201829462150
current and future distribution of aedes aegypti and aedes albopictus (diptera: culicidae) in who eastern mediterranean region.aedes-borne diseases as dengue, zika, chikungunya and yellow fever are an emerging problem worldwide, being transmitted by aedes aegypti and aedes albopictus. lack of up to date information about the distribution of aedes species hampers surveillance and control. global databases have been compiled but these did not capture data in the who eastern mediterranean region (emr), and any models built using these datasets fail to identify highly suitable areas where one or both species may occur. the ...201829444675
a point mutation v419l in the sodium channel gene from natural populations of aedes aegypti is involved in resistance to λ-cyhalothrin in colombia.resistance to pyrethroids in mosquitoes is mainly caused by target site insensitivity known as knockdown resistance (kdr). in this work, we examined the point mutations present in portions of domains i, ii, iii, and iv of the sodium channel gene in aedes aegypti mosquitoes from three colombian municipalities. a partial region coding for the sodium channel gene from resistant mosquitoes was sequenced, and a simple allele-specific pcr-based assay (as-pcr) was used to analyze mutations at the popul ...201829443870
analysis of peptide ligand specificity of different insect adipokinetic hormone receptors.adipokinetic hormone (akh) is a highly researched insect neuropeptide that induces the mobilization of carbohydrates and lipids from the fat body at times of high physical activity, such as flight and locomotion. as a naturally occurring ligand, akh has undergone quite a number of amino acid changes throughout evolution, and in some insect species multiple akhs are present. akh acts by binding to a rhodopsin-like g protein-coupled receptor, which is related to the vertebrate gonadotropin-releasi ...201829439466
functional characterization and quantitative expression analysis of two gnrh-related peptide receptors in the mosquito, aedes aegypti.to cope with stressful events such as flight, organisms have evolved various regulatory mechanisms, often involving control by endocrine-derived factors. in insects, two stress-related factors include the gonadotropin-releasing hormone-related peptides adipokinetic hormone (akh) and corazonin (crz). akh is a pleiotropic hormone best known as a substrate liberator of proteins, lipids, and carbohydrates. although a universal function has not yet been elucidated, crz has been shown to have roles in ...201829432729
history dependence in insect flight decisions during odor tracking.natural decision-making often involves extended decision sequences in response to variable stimuli with complex structure. as an example, many animals follow odor plumes to locate food sources or mates, but turbulence breaks up the advected odor signal into intermittent filaments and puffs. this scenario provides an opportunity to ask how animals use sparse, instantaneous, and stochastic signal encounters to generate goal-oriented behavioral sequences. here we examined the trajectories of flying ...201829432454
carica papaya (papaya) latex: a new paradigm to combat against dengue and filariasis vectors aedes aegypti and culex quinquefasciatus (diptera: culicidae).this study manifests the larvicidal efficacy of carica papaya latex extract and silver nanoparticles (cpagnps) synthesized using latex, against developing immature juveniles of aedes aegypti and culex quinquefasciatus. briefly, the latex was collected and fractioned with different solvents such as chloroform, methanol and aqueously. the obtained crude extracts were subjected to larvicidal activity in the dose-dependent method. after 24 h, the mortality rate was calculated and statistically analy ...201829430347
re-emergence of aedes aegypti in egypt. 201829412959
entomopathogenic fungi and their potential for the management of aedes aegypti (diptera: culicidae) in the americas.classical biological control has been used extensively for the management of exotic weeds and agricultural pests, but never for alien insect vectors of medical importance. this simple but elegant control strategy involves the introduction of coevolved natural enemies from the centre of origin of the target alien species. aedes aegypti - the primary vector of the dengue, yellow fever and zika flaviviruses - is just such an invasive alien in the americas where it arrived accidentally from its west ...201829412361
the influence of a light and dark cycle on the egg laying activity of aedes aegypti (linnaeus, 1762) (diptera: culicidae).the epidemiological importance of the mosquito aedes aegypti as a vector of multiple human pathogens has generated a growing number of studies on the physiology and behaviour of its blood-feeding females. the activity of oviposition is one of the critical elements contributing to the expansion of ae. aegypti's populations. although there is a vast literature about oviposition behaviour, significant specific knowledge about egg viability and female fertility under light and dark conditions is sti ...201829412343
clinical and epidemiological teaching of dengue through simulated practice.to describe the experience of clinical teaching on dengue and the practice of epidemiological surveillance using problematization methodology.201829412306
[eradication of aedes aegypti: new perspectives on international health cooperation in the americas]. 201829412266
context-dependent interactive effects of non-lethal predation on larvae impact adult longevity and body composition.predation impacts development, behavior and morphology of prey species thereby shaping their abundances, distribution and community structure. non-lethal threat of predation, specifically, can have a strong influence on prey lifehistory characteristics. while investigations often focus on the impact of predation threat on prey in isolation, tests of its interactive effects with food availability and resource competition on prey survival and fitness can improve understanding of costs, benefits an ...201829401513
molecular identification, transcript expression, and functional deorphanization of the adipokinetic hormone/corazonin-related peptide receptor in the disease vector, aedes aegypti.the recently discovered adipokinetic hormone/corazonin-related peptide (acp) is an insect neuropeptide structurally intermediate between corazonin (crz) and adipokinetic (akh) hormones, which all demonstrate homology to the vertebrate gonadotropin-releasing hormone (gnrh). to date, the function of the acp signaling system remains unclear. in the present study, we molecularly identified the complete open reading frame encoding the aedes aegypti acp receptor (acpr), which spans nine exons and unde ...201829391531
[the recent epidemic spread of zika virus disease].zika virus (zikv) is one of the members of the spondweni serocomplex within the genus flavivirus of the family flaviviridae. the virus was first isolated from a serum specimen from a sentinel non-human primate in the zika forest of uganda in 1947. zikv is transmitted by aedes aegypti and a. albopictus in an urban cycle and maintained in a sylvatic cycle between aedes mosquitoes and monkeys in africa and asia. initially, the virus was thought to cause only mild and nonspecific clinical symptoms i ...201831105130
mosquito responses to trait- and density-mediated interactions of predation.mosquito and predatory larvae often share the same habitat. predators may influence mosquito prey populations through both lethal effect and non-lethal pathways. a series of experimental manipulations were used to distinguish between lethal (density-mediated interaction) and non-lethal (trait-mediated interaction) effects in a model system comprised of invasive prey mosquito, aedes aegypti, and a predatory mosquito toxorhynchites rutilus. treatments with predators present or manipulations mimick ...201829594613
effect of relaxation of deltamethrin pressure on metabolic resistance in a pyrethroid-resistant aedes aegypti (diptera: culicidae) strain harboring fixed p989p and g1016g kdr alleles.mutation of the voltage-gated sodium channel genes or knockdown resistance (kdr) and metabolic resistance in aedes aegypti (l.) (diptera: culicidae) are important resistance mechanisms against pyrethroids. the present study investigated the effect of relaxation of deltamethrin selection pressure on the level of mixed-function oxidases (mfo), when the allele frequency of s989p+v1016g mutations is fixed in a resistant ae. aegypti strain (upk-r) from chiang mai, thailand. the mosquitoes were divide ...201829590378
maternal invasion history of aedes aegypti and aedes albopictus into the isthmus of panama: implications for the control of emergent viral disease agents.despite an increase in dengue outbreaks and the arrival of chikungunya and zika disease in panama, studies on the demographic history of the invasive aedes mosquitoes that are the principle vectors of these diseases are still lacking in this region. here, we assess the genetic diversity of these mosquitoes in order to decipher their invasion histories into the isthmus of panama. dna sequences from the mitochondrial cytochrome c oxidase i gene obtained from 30 localities in 10 provinces confirmed ...201829579112
frequency and intensity of pyrethroid resistance through the cdc bottle bioassay and their association with the frequency of kdr mutations in aedes aegypti (diptera: culicidae) from mexico.the control of aedes aegypti (l.), the main urban vector that causes arboviral diseases such as dengue, chikungunya and zika, has proved to be a challenge because of a rapid increase in insecticide resistance. therefore, adequate monitoring of insecticide resistance is an essential element in the control of ae. aegypti and the diseases it transmits. we estimated the frequency and intensity (resistance frequency rapid diagnostic test [f-rdt] and resistance intensity rapid diagnostic test [i-rdt]) ...201829575404
population dynamics of engineered underdominance and killer-rescue gene drives in the control of disease vectors.a number of different genetics-based vector control methods have been proposed. two approaches currently under development in aedes aegypti mosquitoes are the two-locus engineered underdominance and killer-rescue gene drive systems. each of these is theoretically capable of increasing in frequency within a population, thus spreading associated desirable genetic traits. thus they have gained attention for their potential to aid in the fight against various mosquito-vectored diseases. in the case ...201829570717
repellent activity of trig (n-n diethyl benzamide) against man-biting mosquitoes.a study was conducted to assess efficacy of a new repellent brand trig (15% n-n diethyl benzamide) when compared to deet (20% n-n methyl toluamide). the repellents were tested in laboratory and field. in the laboratory, the repellence was tested on human volunteers, by exposing their repellent-treated arms on starved mosquitoes in cages for 3 minutes at hourly intervals, while counting the landing and probing attempts. anopheles gambiae and aedes aegypti mosquitoes were used. field evaluation wa ...201829552040
hr38, an ortholog of nr4a family nuclear receptors, mediates 20-hydroxyecdysone regulation of carbohydrate metabolism during mosquito reproduction.the aedes aegypti mosquito is the principal vector for many dangerous human viral diseases. carbohydrate metabolism (cm) is essential for supplying the energy necessary for host seeking, blood digestion and rapid egg development of this vector insect. the steroid hormone 20-hydroxyecdysone (20e) and the ecdysone receptor (ecr) are important regulators of cm, coordinating it with female reproductive events. we report here that the nr4a nuclear receptor ahr38 plays a critical role in mediating the ...201829526770
jh biosynthesis and hemolymph titers in adult male aedes aegypti mosquitoes.juvenile hormone (jh) is a major hormonal regulator in insects. in aedes aegypti females, jh signals the completion of the ecdysis to the adult stage and initiates reproductive processes. although the regulation of jh synthesis and titer in ae. aegypti females has been extensively studied, relatively little is known about changes of jh synthesis and titers in male mosquitoes, as well as on the roles of jh controlling male reproductive biology. a better understanding of male mosquito reproductive ...201829526769
mosquito pornoscopy: observation and interruption of aedes aegypti copulation to determine female polyandric event and mixed progeny.ades aegypti is the most important arbovirus vector in the world, and new strategies are under evaluation. biological studies mentioning the occurrence of a second mate in aedes aegypti can interfere with vector control program planning, which involves male mosquito release technique. this study presents different experiments to show the occurrence of mixed progeny. mixed male crosses (using a combination of different type of males in confinement with virgin females) showed no polyandric female. ...201829518112
comparative oviposition site selection in containers by aedes aegypti and aedes albopictus (diptera: culicidae) from florida.invasive container mosquito species aedes aegypti (l.) and ae. albopictus (skuse) frequently co-occur in nature where they are typically segregated spatially by distinctive macrohabitat preferences that may select for different oviposition strategies. in a standard cage environment, we compared oviposition site selection by individual gravid females of these species exposed to variable numbers of water-holding cups and the presence or absence of a rugose wettable container lining. offered plasti ...201829514300
building infestation index for aedes aegypti and occurrence of dengue fever in the municipality of foz do iguaçu, paraná, brazil, from 2001 to 2016.the building infestation index (bii) uses the rapid assay of the larval index for aedes aegypti (liraa) to express the relationship between positive and surveyed properties. we evaluated liraa and the relationship between the bii and climate variables for dengue cases in foz do iguaçu municipality, paraná.201829513847
occurrence of sweet refuse at disposal sites: rainwater retention capacity and potential breeding opportunities for aedes aegypti.nectar is the staple diet of adult mosquitoes in the wild, but its availability is inconsistent and can be affected by rainfall. in urban centers, aedes vectors commonly use man-made containers as their major habitat; however, they can colonize any items replenished by rainfall. garbage output has increased significantly in recent years, at a time when collection frequency is reducing. such garbage usually includes organic components, some of which are sweet and can be fed upon by other animals ...201829512008
the non-canonical notch signaling is essential for the control of fertility in aedes aegypti.the notch signaling pathway is a highly evolutionarily-conserved cell-cell signaling pathway that regulates many events during development. it plays a pivotal role in the regulation of fundamental cellular processes, such as cell proliferation, stem cell maintenance, and differentiation during embryonic and adult development. however, functions of notch signaling in aedes aegypti, the major mosquito vector for dengue, are largely unknown. in this study, we identified a unique feature of a. aegyp ...201829505577
dengue knowledge in indoor dengue patients from low socioeconomic class; aetiology, symptoms, mode of transmission and prevention.dengue fever has emerged as an emerging public health issue during last decade bearing significant morbidity and economic burden particularly in third world countries. current study aims to assess various domains of knowledge of indoor dengue patients..201829504327
resistance of aedes aegypti (diptera: culicidae) populations to deltamethrin, permethrin, and temephos in cambodia.dengue fever is a major public health concern, including 185,000 annual cases in cambodia. aedes aegypti is the primary vector for dengue transmission and is targeted with insecticide treatments. this study characterized the insecticide resistance status of ae aegypti from rural and urban locations. the susceptibility to temephos, permethrin, and deltamethrin of ae aegypti was evaluated in accordance with world health organization instructions. all the field populations showed lower mortality ra ...201829502428
bacterial microbiota of aedes aegypti mosquito larvae is altered by intoxication with bacillus thuringiensis israelensis.insect microbiota is a dynamic microbial community that can actively participate in defense against pathogens. bacillus thuringiensis (bt) is a natural entomopathogen widely used as a bioinsecticide for pest control. although bt's mode of action has been extensively studied, whether the presence of microbiota is mandatory for bt to effectively kill the insect is still under debate. an association between a higher tolerance and a modified microbiota was already evidenced but a critical point rema ...201829499735
anti-diuretic action of a capa neuropeptide against a subset of diuretic hormones in the disease vector aedes aegypti.the mosquito aedes aegypti is a vector responsible for transmitting various pathogens to humans, and their prominence as chief vectors of human disease is largely due to their anthropophilic blood feeding behaviour. larval stage mosquitoes must deal with the potential dilution of their haemolymph in freshwater, whereas the haematophagus a. aegypti female faces the challenge of excess ion and water intake after a blood meal. the excretory system, composed of the malpighian tubules (mts) and hindg ...201829496779
dengue in araraquara, state of são paulo: epidemiology, climate and aedes aegypti infestation.objective to describe the epidemiology of dengue in a medium-sized city in the state of são paulo. methods data, such as circulating serotypes, severe cases and deaths, age group, sex, among others, were obtained on reported and confirmed dengue cases in araraquara, state of são paulo, between 1991 and 2015. climatic and infestation data were also analyzed. these variables were evaluated descriptively, using statistical measures such as frequencies, averages, minimum and maximum. dengue incidenc ...201829489994
truck-mounted natular 2ec (spinosad) ulv residual treatment in a simulated urban environment to control aedes aegypti and aedes albopictus in north florida.preemptive treatment of dry habitats with an ultra-low volume (ulv) residual larvicide may be effective in an integrated vector management program to control populations of container-inhabiting aedes mosquitoes, key vectors of zika, dengue, and chikungunya viruses. we exposed dry, artificial containers placed in exposed and protected locations to natular 2ec (spinosad) larvicide applied with a truck-mounted ulv sprayer in a simulated urban setting in north florida, and later introduced water and ...201831442121
ultra-low volume application of spinosad (natular 2ec) larvicide as a residual in a tropical environment against aedes and anopheles species.we investigated the efficacy of a liquid larvicide, natular 2ec® (spinosad), applied with ultra-low volume sprayer as a residual application during the dry season in southeastern thailand against 4 medically important species-aedes aegypti, ae. albopictus, anopheles dirus, and an. minimus. we found that this larvicide could be applied as a residual to dry areas known to collect water and potentially still be effective after rains or irrigation, which could increase the flexibility and efficiency ...201831442120
effect of travel speed on dispersion of aqualuer 20-20 sprayed by a truck-mounted ultra-low-volume sprayer against caged aedes aegypti1.the effect of travel speed of a truck-mounted ultra-low-volume (ulv) sprayer on its application efficacy was studied at st. johns county fairground, elkton, fl, during summer 2015. the efficacy was assessed by spray deposition, droplet size spectrum, and 24-h mortality of caged adult aedes aegypti, using 2 rows of sampling locations, 15 m apart and spread up to 122 m from the spray. each location had a bioassay cage and an impinger droplet sampler, 1 m apart from each other, at 1.5 m off the gro ...201831442115
application efficacy of vectobac wdg against larval aedes aegypti using thermal fog technology.the effectiveness of thermal fog application of bacillus thuringiensis israelensis (vectobac® wdg) against larval aedes aegypti was evaluated in open and forest canopied environments in northeastern florida, using a handheld igeba® tf34 thermal fogger (with water conversion kit). the product was applied at the maximum label rate of 1 kg/ha to linear transects, in each environment, containing empty 0.5-liter cups. after application, late-2nd to early-3rd instars of ae. aegypti were added to each ...201831442110
ultra-low volume application of spinosad (natular 2ec) as a residual in a hot-arid environment against aedes aegypti.the invasive aedes aegypti is an important disease vector increasing in frequency in hot-arid regions of the usa such as the southwest. within hot-arid surroundings this mosquito may be confined to peridomestic locations that tend to be cooler and humid, such as in lush, irrigated ornamental vegetation surrounding homes. however, to reach these habitat refugia, ultra-low volume (ulv) applications of insecticides targeting this mosquito must retain efficacy after being sprayed from the air or str ...201831442108
mosquitocidal activity of a naturally occurring isochroman and synthetic analogs from the plant pathogenic fungus, diaporthe eres against aedes aegypti (diptera: culicidae).the culture filtrate of a plant pathogenic fungus that infects english ivy (hedera helix l., araliaceae) was investigated for mosquitocidal constituents by bioassay-guided isolation. the fungus responsible for pathogenic effects on the plant h. helix has been identified as diaporthe eres nitschke by molecular techniques. the mosquito adulticidal constituent in the culture filtrate was identified as 3,4-dihydro-8-hydroxy-3,5-dimethylisocoumarin (1) by spectroscopic techniques. laboratory bioassay ...201829474636
comparative analysis of major mosquito vectors response to seed-derived essential oil and seed pod-derived extract from acacia nilotica.botanical metabolites are increasingly realized as potential replacements to chemical insecticides. in the present study, acacia nilotica seed essential oil and seed pod solvent extracts were tested for bioefficacy against three important types of mosquitoes. mortality was recorded 24 h post-treatment, while smoke toxicity of adult mosquitoes was recorded at 10 min intervals for 40 min. seed pod powder was extracted with different solvents and hydrodistilled seed oil chemical constituents were d ...201829473901
socioeconomic and human behavioral factors associated with aedes aegypti (diptera: culicidae) immature habitat in tucson, az.aedes aegypti (l.; diptera: culicidae) has been established in the southwestern united states for several decades, but relationships between humans and mosquitoes in this arid region are not well-characterized. in august 2012, the outdoor premises of 355 houses within 20 neighborhoods in tucson, arizona were surveyed for containers that could provide larval habitat for ae. aegypti mosquitoes. at the same time, a knowledge, attitudes and practices (kap) questionnaire was administered to a residen ...201829471405
[current distribution and bioecological characterizations of aedes aegypti and aedes albopictus in brazzaville].aedes aegypti is a mosquito that has been known in congo for decades. it has always been involved in the transmission of some arboviruses. ae. albopictus is an invasive species native from asia. its presence was reported in congo in 2009. the proliferation of ae. albopictus, especially in brazzaville, in association with ae. aegypti, have increased the risk of arbovirus transmission. the distribution and bio-ecology of these two species remain poorly studied. we undertook entomological investiga ...201830950593
hold your breath - differential behavioral and sensory acuity of mosquitoes to acetone and carbon dioxide.host seeking in the yellow fever mosquito, aedes aegypti, and the african malaria mosquito, anopheles coluzzii, relies on specific and generic host-derived odorants. previous analyses indicate that the behavioral response of these species depends differentially on the presence of carbon dioxide (co2) and other constituents in human breath for activation and attraction. in this study, we use a flight tube assay and electrophysiological analysis to assess the role of acetone, a major component of ...201931887129
odorant ligands for the co2 receptor in two anopheles vectors of malaria.exhaled co2 is an important host-seeking cue for anopheles mosquitoes, which is detected by a highly conserved heteromeric receptor consisting of three 7-transmembrane proteins gr22, gr23, and gr24. the co2 receptor neuron has been shown to also respond sensitively to a variety of odorants in aedes aegypti. the detection of co2 is important for upwind navigation and for enhancing the attraction to body heat as well as to skin odorants. the orthologs of the co2 receptor proteins are present in ma ...201930796292
nix is a male-determining factor in the asian tiger mosquito aedes albopictus.the initial signal that governs sex determination is highly variable among insects. a homolog of nix, the male-determining factor in aedes aegypti, was previously found in the asian tiger mosquito ae. albopictus. here we show that the ae. albopictus nix (aalnix) is more complex in gene structure and splice isoforms than its ae. aegypti homolog (aaenix). aalnix shows a similar transcription profile compared to aaenix. crispr/cas9-mediated knockouts of aalnix in vivo and in the ae. albopictus c6/3 ...201931901476
geographical distribution of aedes aegypti aegypti and aedes aegypti formosus (diptera: culicidae) in kenya and environmental factors related to their relative abundance.the mosquito aedes aegypti (l.) is the primary vector of various infectious viruses and is typified by a polymorphic color and abundance of white scales on the body. it has been conventionally separated into two subspecies, ae. aeg. formosus (walker) (aaf) and ae. aeg. aegypti (l.) (aaa), with aaf considered a 'sylvan' form and aaa a 'domestic' form. because the two subspecies show different susceptibilities to dengue viruses it is important to understand their distribution. in this study, we co ...201931815285
vgsc-interacting proteins are genetically associated with pyrethroid resistance in aedes aegypti.association mapping of factors that condition pyrethroid resistance in aedes aegypti has consistently identified genes in multiple functional groups. toward better understanding of the mechanisms involved, we examined high throughput sequencing data (hts) from two aedes aegypti aegypti collections from merida, yucatan, mexico treated with either permethrin or deltamethrin. exome capture enrichment for coding regions and the aaegl5 annotation were used to identify genes statistically associated w ...201930695054
susceptibility profile of aedes aegypti l. (diptera: culicidae) from montclair, california, to commonly used pesticides, with note on resistance to pyriproxyfen.the peridomestic anthropophilic aedes aegypti l. (diptera: culicidae) is originated from the wild zoophilic subspecies aedes aegypti formosus in sub-saharan africa, and currently has a broad distribution in human-modified environments of the tropics and subtropics worldwide. in california, breeding populations were initially detected in 2013 in the cities of fresno, madera, and san mateo, and now can be found in 188 cities of 12 counties in the state. recent genetic studies suggest that this spe ...201930810751
dirofilaria and wolbachia in mosquitoes (diptera: culicidae) in central european russia and on the black sea coast.dirofilariasis is endemic in russia, as well as in many other european countries. the aim of this study was to assess the ability of mosquitoes to transfer dirofilaria immitis and dirofilaria repens in regions with temperate and subtropical climates. the possible impact of the symbiotic bacterium wolbachia on dirofilaria transmission was also investigated. 5333 female mosquitoes were collected at 11 points in central european russia and on the black sea coast during the period 2013-2017. out of ...201930644356
lemongrass and cinnamon bark: plant essential oil blend as a spatial repellent for mosquitoes in a field setting.plant essential oils (eos) have been considered as spatial repellents to help disrupt the pathogen transmission cycle of mosquitoes. our objective was to assess spatial repellency effects of eos on the tropical yellow fever mosquito, aedes aegypti (l.) (diptera: culicidae) and on local mosquito populations in coastal british columbia (canada). in laboratory experiments using protocols of the world health organization, three of the solitary eos tested proved repellent to ae. aegypti: cinnamon bar ...201931143941
haemagogus leucocelaenus and haemagogus janthinomys are the primary vectors in the major yellow fever outbreak in brazil, 2016-2018.the yellow fever virus (yfv) caused a severe outbreak in brazil in 2016-2018 that rapidly spread across the atlantic forest in its most populated region without viral circulation for almost 80 years. a comprehensive entomological survey combining analysis of distribution, abundance and yfv natural infection in mosquitoes captured before and during the outbreak was conducted in 44 municipalities of five brazilian states. in total, 17,662 mosquitoes of 89 species were collected. before evidence of ...201930866775
bioefficacy of two nonpyrethroid insecticides for targeted indoor residual spraying against pyrethroid-resistant aedes aegypti.we evaluated the efficacy of bendiocarb (ficam w® 80%) and pirimiphos-methyl (actellic 300cs® 28.16%), applied to different surfaces potentially sprayable within houses during the application of a targeted indoor residual spraying (tirs) against a field pyrethroid-resistant strain of aedes aegypti. bioassays with cones were performed on cement (walls), wood (doors), and textile (cloth) surfaces within typical houses in the mexican city of merida (n = 10). optimal residual efficacy (>80% of mean ...201931922939
discriminating lethal concentrations for transfluthrin, a volatile pyrethroid compound for mosquito control in thailand.pyrethroids are commonly used to control malaria and dengue vectors in thailand. the lack of specific lethal discriminating concentrations (dcs) for specific mosquito species has possibly compromised more accurate assessments of physiological susceptibility to various chemicals over time. previous studies have established dcs of various residual pyrethroids against specific mosquitoes in thailand. however, dcs for transfluthrin (tft), a highly volatile pyrethroid compound, against mosquito vecto ...201931922934
hyalodendrins a and b, new decalin-type tetramic acid larvicides from the endophytic fungus hyalodendriella sp. ponipodef12.two new decalin/tetramic acid hybrid metabolites, hyalodendrins a (1) and b (2) were isolated from plant endophytic fungus hyalodendriella sp. ponipodef12. the structures of the new compounds were elucidated by analysis of the spectroscopic data, including nmr, hrms and ecd, and by chemical conversion. compounds 1 and 2 were phomasetin analogues, and both showed potent larvicidal activity against the fourth-instar larvae of aedes aegypti with the median lethal dose (lc50) values of 10.31 and 5.9 ...201931892246
co-occurrence of kdr mutations v1016i and f1534c and its association with phenotypic resistance to pyrethroids in aedes aegypti (diptera: culicidae) populations from costa rica.aedes aegypti (linnaeus, 1762) is considered the most important mosquito vector species for several arboviruses (e.g., dengue, chikungunya, zika) in costa rica. the primary strategy for the control and prevention of aedes-borne diseases relies on insecticide-based vector control. however, the emergence of insecticide resistance in the mosquito populations presents a significant threat to these prevention actions. the characterization of the mechanisms driving the insecticide resistance in ae. ae ...201931891404
risk of dengue in central africa: vector competence studies with aedes aegypti and aedes albopictus (diptera: culicidae) populations and dengue 2 virus.dengue is the most important mosquito-borne diseases worldwide but was considered scarce in west-central africa. during the last decade, dengue outbreaks have increasingly been reported in urban foci in this region suggesting major epidemiological changes. however, in central africa where both vectors, aedes aegypti and aedes albopictus are well established, the role of each species in dengue transmission remains poorly investigated.201931887138
diverse sensitivities of trpa1 from different mosquito species to thermal and chemical stimuli.temperature and odors profoundly affect the behavior of animals. transient receptor potential channel, subfamily a, member 1 (trpa1) functions as a polymodal nociceptor for sensing both vital environmental cues in insects. mosquitoes are recognized as disease vectors, and many efforts have been devoted to investigations of their host-seeking behaviors and repellents. however, the physiological characteristics of mosquito trpa1 have not been systematically studied. we identified multiple alternat ...201931882848
spatial risk distribution of dengue based on the ecological niche model of aedes aegypti (diptera: culicidae) in the central mexican highlands.dengue is the most important viral disease transmitted by mosquitoes, predominantly aedes (stegomyia) aegypti (l.) (diptera:culicidae). forty percent of the world's population is at risk of contracting the disease, and a large area of mexico presents suitable environmental conditions for the life cycle of ae. aegypti. in particular, the central mexican highlands have a high population density, increasing the risk of transmission and propagation of dengue. in the present study, the potential dist ...201931880769
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