| antheraea pernyi silk fibroin for targeted gene delivery of vegf165-ang-1 with pei. | vascularization is a crucial challenge in tissue engineering. one solution for this problem is to implant scaffolds that contain functional genes that promote vascularization by providing angiogenic growth factors via a gene delivery carrier. poly(ethylenimine) (pei) is a gene delivery carrier with high transfection efficiency but with cytotoxicity. to solve this problem, we utilized antheraea pernyi silk fibroin (asf), which has favorable cytocompatibility and biodegradability, rgd sequences an ... | 2014 | 24867887 |
| diapause induction, color change, and cold tolerance physiology of the diapausing larvae of the chouioia cunea (hymenoptera: eulophidae). | the chalcid wasp chouioia cunea yang (hymenoptera: eulophidae) is one of the most dominant pupal parasitoids of hyphantria cunea (drury) (lepidoptera: arctiidae), an invasive pest of many forestry trees and agricultural crops. for mass rearing c. cunea for biological control purposes, the pupae of antheraea pernyi (guérin-méneville) (lepidoptera: saturniidae) have been widely used as a substitute host in china. in this article, photoperiodic effect on diapause induction in c. cunea within the pu ... | 2014 | 25527599 |
| comparative genetic diversity and genetic structure of three chinese silkworm species bombyx mori l. (lepidoptera: bombycidae), antheraea pernyi guérin-meneville and samia cynthia ricini donovan (lepidoptera: saturniidae). | the genetic diversity and genetic structure of three chinese silkworm species bombyx mori l., antheraea pernyi guérin-meneville and samia cynthia ricini donovan were comparatively assessed based on rapd markers. at the species level, a. pernyi and b. mori showed high levels of genetic diversity, whereas s. cynthia ricini showed low level of genetic diversity. however, at the strain level, a. pernyi had relatively highest genetic diversity and b. mori had lowest genetic diversity. analysis of mol ... | 2011 | 21271066 |
| natural non-mulberry silk nanoparticles for potential-controlled drug release. | natural silk protein nanoparticles are a promising biomaterial for drug delivery due to their pleiotropic properties, including biocompatibility, high bioavailability, and biodegradability. chinese oak tasar antheraea pernyi silk fibroin (apf) nanoparticles are easily obtained using cations as reagents under mild conditions. the mild conditions are potentially advantageous for the encapsulation of sensitive drugs and therapeutic molecules. in the present study, silk fibroin protein nanoparticles ... | 2016 | 27916946 |
| transgenic citrus expressing synthesized cecropin b genes in the phloem exhibits decreased susceptibility to huanglongbing. | expression of synthesized cecropin b genes in the citrus phloem, where candidatus liberibacter asiaticus resides, significantly decreased host susceptibility to huanglongbing. huanglongbing (hlb), associated with candidatus liberibacter asiaticus bacteria, is the most destructive disease of citrus worldwide. all of the commercial sweet orange cultivars lack resistance to this disease. the cationic lytic peptide cecropin b, isolated from the chinese tasar moth (antheraea pernyi), has been shown t ... | 2017 | 27866312 |
| n-acetyltransferase (nat) is a critical conjunct of photoperiodism between the circadian system and endocrine axis in antheraea pernyi. | since its discovery in 1923, the biology of photoperiodism remains a mystery in many ways. we sought the link connecting the circadian system to an endocrine switch, using antheraea pernyi. per-, clk- and cyc-ir were co-expressed in two pairs of dorsolateral neurons of the protocerebrum, suggesting that these are the circadian neurons that also express melatonin-, nat- and hiomt-ir. the results suggest that a melatonin pathway is present in the circadian neurons. melatonin receptor (mt2 or mel-1 ... | 2014 | 24667367 |
| biomimetic nucleation of hydroxyapatite crystals mediated by antheraea pernyi silk sericin promotes osteogenic differentiation of human bone marrow derived mesenchymal stem cells. | biomacromolecules have been used as templates to grow hydroxyapatite crystals (haps) by biomineralization to fabricate mineralized materials for potential application in bone tissue engineering. silk sericin is a protein with features desirable as a biomaterial, such as increased hydrophilicity and biodegradation. mineralization of the silk sericin from antheraea pernyi (a. pernyi) silkworm has rarely been reported. here, for the first time, nucleation of haps on a. pernyi silk sericin (as) was ... | 2014 | 24666022 |
| genetic characterization of a novel iflavirus associated with vomiting disease in the chinese oak silkmoth antheraea pernyi. | larvae of the chinese oak silkmoth (antheraea pernyi) are often affected by avd (a. pernyi vomiting disease), whose causative agent has long been suspected to be a virus. in an unrelated project we discovered a novel positive sense single-stranded rna virus that could reproduce avd symptoms upon injection into healthy a. pernyi larvae. the genome of this virus is 10,163 nucleotides long, has a natural poly-a tail, and contains a single, large open reading frame flanked at the 5' and 3' ends by u ... | 2014 | 24637949 |
| diapause-associated protein3 functions as cu/zn superoxide dismutase in the chinese oak silkworm (antheraea pernyi). | to better understand the molecular mechanism underlying of diapause in antheraea pernyi (a. pernyi), we cloned a novel diapause-associated protein 3 (dap3) gene from a. pernyi by reverse transcription-polymerase chain reaction (rt-pcr) and studied the biological functions. sequence analysis revealed that this gene encodes 171 amino acids and has a conserved domain of copper/zinc superoxide dismutase (cu/zn-sod). western blot and qrt-pcr results showed that dap3 was mainly expressed in the pupal ... | 2014 | 24613963 |
| assessment of freestanding membranes prepared from antheraea pernyi silk fibroin as a potential vehicle for corneal epithelial cell transplantation. | freestanding membranes created from bombyx mori silk fibroin (bmsf) offer a potential vehicle for corneal cell transplantation since they are transparent and support the growth of human corneal epithelial (hce) cells. fibroin derived from the wild silkworm antheraea pernyi (apsf) might provide a superior material by virtue of containing putative cell-attachment sites that are absent from bmsf. thus we have investigated the feasibility of producing transparent, freestanding membranes from apsf an ... | 2014 | 24565906 |
| a novel fibrin(ogen)olytic trypsin-like protease from chinese oak silkworm (antheraea pernyi): purification and characterization. | a novel fibrin(ogen)olytic protease from antheraea pernyi (important economically insect), named cocoonase, was isolated by a combination of ion-exchange chromatography and gel filtration. furthermore, the characterization of cocoonase was investigated using fibrin(ogen)olytic, thrombolysis, and hemorrhagic assays. the nh2-terminal sequence (ivggy svtid kapyq) was established by edman degradation. based on the n-terminal sequencing, cocoonase cdna has been cloned by means of rt-pcr and 5'race. i ... | 2014 | 24491553 |
| serotonin receptor b may lock the gate of ptth release/synthesis in the chinese silk moth, antheraea pernyi; a diapause initiation/maintenance mechanism? | the release of prothoracicotropic hormone, ptth, or its blockade is the major endocrine switch regulating the developmental channel either to metamorphosis or to pupal diapause in the chinese silk moth, antheraea pernyi. we have cloned cdnas encoding two types of serotonin receptors (5htra and b). 5htra-, and 5htrb-like immunohistochemical reactivities (-ir) were colocalized with ptth-ir in two pairs of neurosecretory cells at the dorsolateral region of the protocerebrum (dl). therefore, the cau ... | 2013 | 24223937 |
| mineralization and biocompatibility of antheraea pernyi (a. pernyi) silk sericin film for potential bone tissue engineering. | this study aimed to investigate the mineralization of antheraea pernyi (a. pernyi) silk sericin. mineralization of a. pernyi sericin was performed by alternative soaking in calcium and phosphate. the inhibition of precipitation of calcium carbonate and von kossa staining on a. pernyi sericin were tested, and the corresponding results prove that a. pernyi sericin has ca binding activity. scanning electron microscope (sem) observation shows that spherical crystals could be nucleated on the a. pern ... | 2014 | 24211968 |
| characterization of mitochondrial genome of chinese wild mulberry silkworm, bomyx mandarina (lepidoptera: bombycidae). | the complete mitochondrial genome of chinese bombyx mandarina (chbm) was determined. the circular genome is 15682 bp long, and contains a typical gene complement, order, and arrangement identical to that of bombyx mori (b. mori) and japanese bombyx mandarina (jabm) except for two additional trna-like structures: trna( ser(tga))-like and trn( aile(tat))-like. all protein-coding sequences are initiated with a typical atn codon except for the coi gene, which has a 4-bp ttag putative initiator codon ... | 2008 | 18677597 |
| nucleation of hydroxyapatite on antheraea pernyi (a. pernyi) silk fibroin film. | antheraea pernyi (a. pernyi) silk fibroin, which is spun from a wild silkworm, has increasingly attracted interest in the field of tissue engineering. the aim of this study was to investigate the nucleation of hydroxyapatite (hap) on a. pernyi fibroin film. von kossa staining proved that a. pernyi fibroin had ca binding activity. the a. pernyi fibroin film was mineralized with hap crystals by alternative soaking in calcium and phosphate solutions. spherical crystals were nucleated on the a. pern ... | 2014 | 24211958 |
| screening of candidate proteins interacting with ie-2 of bombyx mori nucleopolyhedrovirus. | ie-2 of bombyx mori nucleopolyhedrovirus (bmnpv) has been shown to play important roles in baculovirus infection, which are involved in gene expression and viral replication. however, the mechanism remains unknown. in this paper, by targetp software, four genes, i.e.-2, odv-e26, odv-e56 and bmnpv-gp101 (ac-orf116) of bmnpv and autographa californica multiple npv (acmnpv) were predicted to be located in mitochondria. by blast tool using bmnpv ie-2 protein sequence, 14 npvs were found to have ie-2 ... | 2013 | 24078157 |
| molecular cloning and characterization of two 12 kda fk506-binding protein genes in the chinese oak silkworm, antheraea pernyi. | two 12 kda fk506-binding protein (fkbp12) genes were isolated and characterized from chinese oak silkworm antheraea pernyi , an important agricultural and edible insect, designated apfkbp12 a and b, respectively. both apfkbp12 a and b contained 108 amino acids with 82% sequence identity. phylogenetic analysis showed that fkbp12 b sequences of a. pernyi, bombyx mori , and danaus plexippus were clearly separated from fkbp12 a sequences of these three species, suggesting that insect fkbp12 a and b ... | 2013 | 23617895 |
| insight into the structure of single antheraea pernyi silkworm fibers using synchrotron ftir microspectroscopy. | synchrotron ftir (s-ftir) microspectroscopy was used to monitor both protein secondary structures (conformations) and their orientations in single cocoon silk fibers of the chinese tussah silk moth ( antheraea pernyi ). in addition, to understand further the relationship between structure and properties of single silk fibers, we studied the changes of orientation and content of different secondary structures in single a. pernyi silk fibers when subjected to different strains. the results showed ... | 2013 | 23607809 |
| [research for the production of recombinant human epidermal growth factor using samia cynthia ricini pupae bioreactor]. | the protein production system using a baculovirus antheraea pernyi nucleopolyhedrovirus (anpenpv) as a gene expression vector and its host insect as a natural bioreactor was successful established and its excellent performance in the protein production has been demonstrated. in this paper, the system is used to produce recombinant human epidermal growth factor (rhegf), which have been widely used in medical and cosmetic treatment. a recombinant anpehegf virus has been constructed by replacing th ... | 2013 | 23488154 |
| analysis of the genomic sequence of philosamia cynthia nucleopolyhedrin virus and comparison with antheraea pernyi nucleopolyhedrin virus. | two species of wild silkworms, the chinese oak silkworm (antheraea pernyi) and the castor silkworm philosamia cynthia ricini, can acquire a serious disease caused by nucleopolyhedrin viruses (npvs) (known as anpenpv and phcynpv, respectively). the two viruses have similar polyhedral morphologies and their viral fragments share high sequence similarity. however, the physical maps of the viral genomes and cross-infectivity of the viruses are different. the genome sequences of two anpenpv isolates ... | 2013 | 23425301 |
| cloning, expression and phylogenetic analysis of hemolin, from the chinese oak silkmoth, antheraea pernyi. | the chinese oak silk moth antheraea pernyi is an important silk producer. to understand microbial resistance of this moth, we cloned hemolin, encoding a multifunctional immune protein belonging to the immunoglobulin superfamily, and examined the expression in gonads and fat body. the aphemolin amino acid sequence was compared to other hemolin sequences in order to predict functional sites. several sites were conserved; among them a phosphate binding site, which according to 3d structure modellin ... | 2005 | 15978282 |
| the expression of gfp under the control of fibroin promotor in primary ovarian cells of antheraea pernyi. | the fibroin promoter can stably express foreign gene in lepidopteran cells. total rna was extracted from the gland of silkworm, antheraea pernyi and the transcription initiation site of fibroin gene of a. pernyi was identified by rna ligase mediated rapid amplification of cdna ends (rlm-race). the expression vector (pgfp-n2/fib) was constructed by use of replacing the cmv promoter with the fibroin promoter. the results of visual screening under a fluorescent inverted microscope and western blot ... | 2003 | 14660868 |
| the silkmoth cocoon as humidity trap and waterproof barrier. | to better understand how silkmoth cocoons maintain the correct internal moisture levels for successful pupation, we examined cocoons from the long-domesticated mulberry silkmoth bombyx mori as well as from two wild silkmoth species, antheraea pernyi and philosamia cynthia ricini. we determined fluid-independent values for the porosity, tortuosity and permeability of the inner and outer surfaces of cocoons. permeabilities were low and, with the exception of a. pernyi cocoons, inner surfaces were ... | 2013 | 23388210 |
| incorporation of exogenous rgd peptide and inter-species blending as strategies for enhancing human corneal limbal epithelial cell growth on bombyx mori silk fibroin membranes. | while fibroin isolated from the cocoons of domesticated silkworm bombyx mori supports growth of human corneal limbal epithelial (hle) cells, the mechanism of cell attachment remains unclear. in the present study we sought to enhance the attachment of hle cells to membranes of bombyx mori silk fibroin (bmsf) through surface functionalization with an arginine-glycine-aspartic acid (rgd)-containing peptide. moreover, we have examined the response of hle cells to bmsf when blended with the fibroin p ... | 2013 | 24955953 |
| patterns of period and pigment-dispersing hormone immunoreactivity in the brain of the european honeybee (apis mellifera): age- and time-related plasticity. | we explored the neural basis of age- and task-related plasticity in circadian patterns of activity in the honeybee. to identify putative circadian pacemakers in the bee brain, we used antibodies against drosophila melanogaster and antheraea pernyi period and an antiserum to crustacean pigment-dispersing hormone (pdh) known to cross-react with insect pigment-dispersing factors (pdfs). in contrast to previous results from drosophila, pdh and per immunoreactivity (-ir) were not colocalized in bee n ... | 2003 | 12900924 |
| [investigation of rat submandibular gland cells and antheraea pernyi silk fibroin films co-culture in vitro]. | in this study, the morphology, attachment, growth and secretion of rat submandibular gland cells (rsmgs) cultured on the antheraea pernyi silk fibroin(apsf) films were observed in vitro. | 2012 | 23135109 |
| characterization of arylalkylamine n-acetyltransferase from silkmoth (antheraea pernyi) and pesticidal drug design based on the baculovirus-expressed enzyme. | arylalkylamine n-acetyltransferase (aanat; ec 2.3.1.87) catalyzes the n-acetylation of arylalkylamines. a cdna encoding aanat (apaanat) was cloned from antheraea pernyi by pcr. the cdna of 1966 bp encodes a 261 amino acid protein. the amino acid sequence was found to have a high homology with bombyx mori aanat (bmnat) but had very low homology with vertebrate aanats. amino acid sequence analysis revealed that four insect aanats cloned from three species including apaanat formed a distinct cluste ... | 2013 | 23064182 |
| involvement of a versatile pattern recognition receptor, apolipophorin-iii in prophenoloxidase activation and antibacterial defense of the chinese oak silkworm, antheraea pernyi. | apolipophorin-iii (apolp-iii) is an exchangeable apolipoprotein found in many insect species and functions as a lipid transport vehicle. recent studies have shown that apolp-iii is a multifunctional molecule involved in not only lipid transportation but also innate immune responses. in the present study, the pattern recognition properties of antheraea pernyi apolp-iii were investigated. recombinant ap-apolp-iii was bound to different species of microbes and further study showed the rap-apolp-iii ... | 2016 | 27387151 |
| supercritical carbon dioxide extraction of the oak silkworm (antheraea pernyi) pupal oil: process optimization and composition determination. | supercritical carbon dioxide (sc-co(2)) extraction of oil from oak silkworm pupae was performed in the present research. response surface methodology (rsm) was applied to optimize the parameters of sc-co(2) extraction, including extraction pressure, temperature, time and co(2) flow rate on the yield of oak silkworm pupal oil (ospo). the optimal extraction condition for oil yield within the experimental range of the variables researched was at 28.03 mpa, 1.83 h, 35.31 °c and 20.26 l/h as flow rat ... | 2012 | 22408458 |
| synchrotron ftir microspectroscopy of single natural silk fibers. | synchrotron ftir (s-ftir) microspectroscopy was used to monitor the silk protein conformation in a range of single natural silk fibers (domestic and wild silkworm and spider dragline silk). with the selection of suitable aperture size, we obtained high-resolution s-ftir spectra capable of semiquantitative analysis of protein secondary structures. for the first time, we have determined from s-ftir the β-sheet content in a range of natural single silk fibers, 28 ± 4, 23 ± 2, and 17 ± 4% in bombyx ... | 2011 | 21790142 |
| molecular cloning, expression pattern and phylogenetic analysis of the will die slowly gene from the chinese oak silkworm, antheraea pernyi. | the will die slowly (wds) gene coding for a wd-repeat protein with seven repeats has been characterized in drosophila melanogaster. in this paper, the wds gene was isolated and characterized from the chinese oak silkworm, antheraea pernyi (lepidoptera: saturniidae). the obtained 1733 bp cdna sequence contains an open reading frame of 1041 bp encoding a polypeptide of 346 amino acids, with 85% sequence identity to that from d. melanogaster. rt-pcr analysis showed that the wds gene was transcribed ... | 2011 | 21104437 |
| characterization of tyrosine-rich antheraea pernyi silk fibroin hydrolysate. | antheraea pernyi silk fibroin (sf) hydrolysate were characterized using uv-vis spectrometer, amino acid composition and heavy metal contents to explore its potential sources for food or cosmetic additives. the hydrolyzed a. pernyi sf was separated into two parts: (a) sfa, alanine-rich fraction and (b) sfb, tyrosine-rich fraction. sfb exhibited strong absorption peaks at 210 and 280 nm due to the presence of the tyrosine. heavy metal analysis showed that arsenic and mercury did not detect. other ... | 2011 | 20937302 |
| flies, clocks and evolution. | the negative feedback model for gene regulation of the circadian mechanism is described for the fruitfly, drosophila melanogaster. the conservation of function of clock molecules is illustrated by comparison with the mammalian circadian system, and the apparent swapping of roles between various canonical clock gene components is highlighted. the role of clock gene duplications and divergence of function is introduced via the timeless gene. the impressive similarities in clock gene regulation bet ... | 2001 | 11710984 |
| antheraea pernyi silk fiber: a potential resource for artificially biospinning spider dragline silk. | the outstanding properties of spider dragline silk are likely to be determined by a combination of the primary sequences and the secondary structure of the silk proteins. antheraea pernyi silk has more similar sequences to spider dragline silk than the silk from its domestic counterpart, bombyx mori. this makes it much potential as a resource for biospinning spider dragline silk. this paper further verified its possibility as the resource from the mechanical properties and the structures of the ... | 2010 | 20454537 |
| the mitochondrial genome of the butterfly papilio xuthus (lepidoptera: papilionidae) and related phylogenetic analyses. | the nearly complete mitochondrial genome of the butterfly papilio xuthus (lepidoptera: papilionidae) was sequenced for its nucleotide sequence of 13,964 bp. the genome has a typical gene order identical to other lepidopteran species. all trnas showed same stable canonical clover-leaf structure as those of other insects, except for trna(ser) (agn), in which the dihydrouracil arm (dhu arm) could not form stable stem-loop structure. anomalous initiation codons have been observed for the cox1 gene, ... | 2010 | 20213506 |
| the complete mitogenome and phylogenetic analysis of bombyx mandarina strain qingzhou. | the complete nucleotide sequence of the mitogenome of bombyx mandarina strain qingzhou was determined. the circular genome is 15,717 bp long and has the typical gene organization and order of lepidopteran mitogenomes. all protein-coding sequences are initiated with a typical atn codon, except the coi gene, which has a 4-bp ttag putative initiator codon. eleven of the 13 protein-coding gene have a complete termination codon (all taa), but the remaining two genes terminate with incomplete codons. ... | 2010 | 19728146 |
| construction of a full-length cdna library from chinese oak silkworm pupa and identification of a kk-42-binding protein gene in relation to pupa-diapause termination. | in this study we successfully constructed a full-length cdna library from chinese oak silkworm, antheraea pernyi, the most well-known wild silkworm used for silk production and insect food. total rna was extracted from a single fresh female pupa at the diapause stage. the titer of the library was 5 x 10(5) cfu/ml and the proportion of recombinant clones was approximately 95%. expressed sequence tag (est) analysis was used to characterize the library. a total of 175 clustered ests consisting of 2 ... | 2009 | 19564928 |
| the presence of monoglucosylated n196-glycan is important for the structural stability of storage protein, arylphorin. | although n-glycosylation has been known to increase the stability of glycoproteins, it is difficult to assess the structural importance of glycans in the stabilization of glycoproteins. apa (antheraea pernyi arylphorin) is an insect hexamerin that has two n-glycosylations at asn196 and asn344 respectively. the glycosylation of asn344 is critical for the folding process; however, glycosylation of asn196 is not. interestingly, the n196-glycan (glycosylation of asn196) remains in an immature form ( ... | 2009 | 19358695 |
| upregulated proteins associated with fibroin synthesis in posterior silk glands of antheraea pernyi fifth instar larvae. | antheraea pernyi is a typical wild silkworm with larval silk glands specific for the synthesis and secretion of silk proteins. we examined changes in the protein profile in the posterior section of the silk glands on days 1 and 4 of the last larval instar. two-dimensional gel electrophoresis over the ph range of 4-7 followed by silver staining revealed about 350 protein spots. twenty-three proteins upregulated on day 4 were analyzed using matrix-assisted laser desorption/ionization time-of-fligh ... | 2009 | 20403748 |
| the complete mitochondrial genome of the chinese oak silkmoth, antheraea pernyi (lepidoptera: saturniidae). | we determined the complete nucleotide sequence of the mitogenome from chinese oak silkmoth, antheraea pernyi (lepidoptera: saturniidae). the 15,566 bp circular genome contains a typical gene organization and order for lepidopteran mitogenomes. the mitogenome contains the lowest a+t content (80.16%) among the known lepidopteran mitogenome sequences. an unusual feature is the occurrence of more ts than as, with a slightly negative at skewness (-0.021), in the composition of the major genome strand ... | 2008 | 18685785 |
| g-protein activation, identification and immunolocalization in pheromone-sensitive sensilla trichodea of moths. | electrophysiological in situ recordings from pheromone-sensitive sensilla trichodea of bombyx mori males with a recording pipette which contained g-protein-activating fluoride, showed receptor cell activity similar to that evoked by pheromone stimulation. this suggests that g-proteins might be physiologically active in olfactory sensilla of insects in situ. biochemical experiments using specific antibodies revealed the presence of g-protein, belonging to the gq family, in antennal preparations. ... | 1997 | 9042782 |
| complete nucleotide sequence and organization of the mitogenome of the silk moth caligula boisduvalii (lepidoptera: saturniidae) and comparison with other lepidopteran insects. | the 15,360-bp long complete mitogenome of caligula boisduvalii possesses a gene arrangement and content identical to other completely sequenced lepidopteran mitogenomes, but different from the common arrangement found in most insect order, as the result of the movement of trna(met) to a position 5'-upstream of trna ile. the 330-bp a+t-rich region is apparently capable of forming a stem-and-loop structure, which harbors the conserved flanking sequences at both ends. dissimilar to what has been se ... | 2008 | 18337026 |
| the genome sequence of the multinucleocapsid nucleopolyhedrovirus of the chinese oak silkworm antheraea pernyi. | the complete genome sequence of the liaoning isolate of the antheraea pernyi multinucleocapsid nucleopolyhedrovirus (anpemnpv) was determined. the viral genome size is 126,246 bp, relatively gc-rich (53.5% g+c), with 145 predicted open reading frames (orfs) of more than 50 amino acids, accounting for more than 97% of the genome. 97% of the orfs have predicted functions or homologues in other baculoviruses. there are six homologous repeat regions (hrs) and two bro homologues, which are associated ... | 2007 | 17540430 |
| circadian clock neurons in the silkmoth antheraea pernyi: novel mechanisms of period protein regulation. | we examined period (per) protein regulation in the brain of the silkmoth antheraea pernyi. per expression is restricted to the cytoplasm and axons of eight neurons, with no evidence of temporal movement into the nucleus. these neurons appear to be circadian clock cells, because per and per mrna are colocalized and their levels oscillate in these cells, timeless protein immunoreactivity is coexpressed in each per-positive neuron, and clock protein and mrna oscillations are all suppressed in these ... | 1996 | 8938121 |
| structure and properties of regenerated antheraea pernyi silk fibroin in aqueous solution. | antheraea pernyi silk fibroin fibers were dissolved by aqueous lithium thiocyanate to obtain regenerated a. pernyi silk fibroin solution. by means of circular dichroism, (13)c nmr and raman spectroscopy, the molecular conformation of regenerated a. pernyi silk fibroin in aqueous solution was investigated. the relationship of environmental factors and sol-gel transformation behavior of regenerated a. pernyi silk fibroin was also studied. the molecular conformations of regenerated a. pernyi silk f ... | 2007 | 17173967 |
| using solvents with different molecular sizes to investigate the structure of antheraea pernyi silk. | the interaction between silk and polar solvents of different molecular size can be an important tool for understanding the structural features of natural silk; in particular, the disordered regions associated with the key property of mechanical toughness. in this work, we investigate the transitions induced in the tensile performance and structure of as-reeled antheraea pernyi silks from different silkworms by a range of solvents that can only soften the protein chains in the amorphous regions. ... | 2013 | 24044634 |
| the potential of trehalose to replace insect hemolymph in artificial media for trichogramma dendrolimi (hymenoptera: trichogrammatidae). | insect additives have been shown to improve the value of artificial media for trichogramma species, but at the same time maintain dependence on parallel cultures of host insects. in the present study, trichogramma dendrolimi matsumura was reared in vitro from egg to adult on artificial media with different contents of pupal hemolymph of chinese oak silkmoth antheraea pernyi (guérin-méneville) and with supplements of distilled water, or of trehalose dissolved in water or in grace's insect medium. ... | 2013 | 23955927 |
| molecular phylogeny of silkmoths reveals the origin of domesticated silkmoth, bombyx mori from chinese bombyx mandarina and paternal inheritance of antheraea proylei mitochondrial dna. | molecular phylogeny of some of the economically important silkmoths was derived using three mitochondrial genes, 12s rrna, 16s rrna, and coi, and the control region (cr). maximum likelihood (ml) analyses showed two distinct clades, one consisting of moths from bombycidae family and the other from saturniidae family. the mitochondrial cr showed length polymorphisms with indels. the ml analyses for complete mitochondrial genome sequences of bombyx mori (strains aojuku, c108, backokjam, and xiafang ... | 2006 | 16644243 |
| molecular cloning and characterization of a cdna encoding a novel cuticle protein from the chinese oak silkmoth, antheraea pernyi. | in our research to identify gene involved in the cuticle protein, we cloned a novel cuticle protein gene, apcp13, from the chinese oak silkmoth, antheraea pernyi, larvae cdna library. the apcp13 gene encodes a 120 amino acid polypeptide with a predicted molecular mass of 13 kda and a pi of 4.01, and is intron-less gene. the apcp13 contained a type-specific consensus sequence identifiable in other insect cuticle proteins and the deduced amino acid sequence of the apcp13 cdna is most homologous to ... | 2005 | 16323268 |
| [androgen-like and anabolic action of antheraea pernyi guerin-meneville pas]. | it has been found that the ethyl acetate extract isolated from antheraea pernyi pas is able to increase the weight of prostate-semina and levator ani muscle-bulbocavarnosus muscle of castrated mice. in addition, the extract also accelerates the growth of younger male mice and enhances the contents of rna, dna and protein in the liver tissue of mice. it has been determined that beta-ecdysone is one of the effective constituents for the androgen-like and anabolic action. | 1991 | 1713769 |
| cloning of genomic and complementary dna encoding insect pheromone binding proteins: evidence for microdiversity. | genomic dna from the silk moth antheraea pernyi bearing the gene of a pheromone binding protein has been isolated from a partial genomic library using specific cdna probes. the dna spans 3.5 kilobases, contains three exons and two intervening sequences that interrupt the protein coding region of the gene. a dna fragment of a second gene was isolated and the complete primary structure of a corresponding cdna clone was unravelled. the expression of two different genes, giving rise to different phe ... | 1991 | 2001401 |
| primary structure of a pheromone-binding protein from antheraea pernyi: homologies with other ligand-carrying proteins. | an antennal cdna clone encoding the complete sequence (163 amino acids) of a pheromone-binding protein precursor from the male silk moth, antheraea pernyi, was isolated using oligonucleotide probes. the cloned cdna was expressed and the translation product detected by specific antibodies. the deduced protein sequence consists of a signal peptide of 21 amino acids and a mature binding protein of 142 amino acid residues. the predicted structure of this protein is homologous to binding-proteins fro ... | 1990 | 2097299 |
| a cuticle protein gene from the japanese oak silkmoth, antheraea yamamai: gene structure and mrna expression. | a cuticle protein gene, aycp12, from the japanese oak silkmoth, antheraea yamamai, was isolated and characterized. the gene spans 1107 bp and consists of one intron and two exons coding for a 112 amino acid polypeptide with a predicted molecular mass of 12,163 da and a pi of 4.4. the aycp12 protein contained a type-specific consensus sequence identifiable in other insect cuticle proteins and the deduced amino acid sequence of the aycp12 cdna is most homologous to another silkmoth, a. pernyi, cut ... | 2005 | 16231223 |
| compliant film of regenerated antheraea pernyi silk fibroin by chemical crosslinking. | a compliant film was prepared by chemical crosslinking of fibroin from silk fiber of wild silkworm, antheraea pernyi. the silk fiber was dissolved in concentrated aqueous lithium thiocyanate and desalinated by dialysis. the film was cast from the regenerated aqueous solution, and crosslinked by polyethylene glycol diglycidyl ether (peg-de). this film showed high water resistively while maintaining random coil and alpha-helix structure, unlike films prepared by organic solvent treatment that caus ... | 2003 | 12957312 |
| studies on the sites expressing evolutionary changes in the structure of eukaryotic 5s ribosomal rna. | we have determined the complete sequences of 5s rrnas from a lamprey (lampetra reissneri), a lancelet (branchiostoma belcheri), silkworms (philosamia cynthia ricini, bombyx mori, antheraea pernyi), and a silkworm hybrid (artificially fertilized hybrid species of philosamia cynthia ricini male x bombyx mori female), as well as those of cotton seeds (gossypium hirsutum l.). having compared more than 170 eukaryotic 5s rrnas of which seven sequences have been determined by our group as mentioned abo ... | 1988 | 3146644 |
| construction of targeting vector and expression of green fluorescent protein in the silkworm, antheraea pernyi. | the transcription initiation site of anrheraea pernyi was identified, and a gene targeting vector was constructed. the green fluorescent protein (gfp) gene was inserted into this vector under the control of a fibroin promoter. this recombinant vector was used to target the gfp gene to the fibroin region of the silkworm genome. the dna was injected into the testes during the pupae, and was also transferred into the egg via the sperm during fertilization. the analysis showed that the gfp gene was ... | 2003 | 12932302 |
| constructing a feedback loop with circadian clock molecules from the silkmoth, antheraea pernyi. | circadian clocks are important regulators of behavior and physiology. the circadian clock of drosophila depends on an autoinhibitory feedback loop involving dclock, cycle (also called dbmal, for drosophila brain and muscle arnt-like protein), dperiod, and dtimeless. recent studies suggest that the clock mechanism in other insect species may differ strikingly from that of drosophila. we cloned clock, bmal, and timeless homologs (apclock, apbmal, and aptimeless) from the silkmoth antheraea pernyi, ... | 2003 | 12869551 |
| [studies on protein-based identification method of genetically modified capsicum]. | the detection system based on protein is a method to evaluate the safety of genetically modified foods (gmf). using cecropin bd gene in capsicum, a detecting method was set up. it is a system of evaluating the real expressive condition and safety of the foreign target protein of gmf. in this studies, with the preformative technic method, a satisfactory results by making use of hemolymph of immunized pupae of antheraea pernyi as standard experimental material was achieved, comparing with the real ... | 2003 | 12793005 |
| changes in brain monoamine contents in diapause pupae of antheraea pernyi when activated under long-day and by chilling. | the contents of biogenic monoamines in the brain-subesophageal ganglion complex were measured in both bivoltine and univoltine strains of the chinese oak silkmoth, antheraea pernyi using hplc-ecd. the contents in the bivoltine strain were monitored every five days under ld 12:12 and 16:8, photoperiods maintaining and terminating diapause, respectively at 25 degrees c. the univoltine strain was chilled at 2 degrees c and the monoamine contents were measured at 10-day intervals. dopamine, norepine ... | 2002 | 12770054 |
| uptake of atmospheric carbon dioxide into silk fiber by silkworms. | the relation between the uptake of atmospheric co(2) and insect's production of silk fiber has not yet been reported. here, we provide the first quantitative demonstrations that four species of silkworms (bombyx mori, samia cynthia ricini, antheraea pernyi, and antheraea yamamai) and a silk-producing spider (nephila clavata) incorporate atmospheric co(2) into their silk fibers. the abundance of (13)c incorporated from the environment was determined by mass spectrometry and (13)c nmr measurements ... | 2013 | 12741798 |
| bulla gouldiana period exhibits unique regulation at the mrna and protein levels. | the authors cloned the period (per) gene from the marine mollusk bulla gouldiana, a well-characterized circadian model system. this allowed them to examine the characteristics of the per gene in a new phylum, and to make comparisons with the conserved per domains previously characterized in insects and vertebrates. only one copy of the per gene is present in the bulla genome, and it is most similar to per in two insects: the cockroach, periplaneta americana, and silkmoth, antheraea pernyi. compa ... | 2002 | 12375618 |
| the cdna-structure of the prothoracicotropic hormone (ptth) of the silkmoth hyalophora cecropia. | the structure of the prothoracicotropic neurohormone (ptth) of the silkmoth hyalophora cecropia was elucidated at the cdna level. the identified cdna of 803 nt, which is over 90% identical with the corresponding part of the samia cynthia ricini ptth gene, encodes a preprohormone of 240 amino acids. presence of proteolytic cleavage sites indicates that the preprohormone is split into a signal peptide, an intercalated peptide (64 residues), and the ptth monomer (125 residues). preprohormones of h. ... | 2002 | 11755069 |
| cdna cloning and expression pattern of two enolase genes from the chinese oak silkworm, antheraea pernyi. | in this study, two enolase genes were isolated and characterized from the chinese oak silkworm, antheraea perny, which were designated as enolase i and ii, respectively. the enolase i cdna sequence was 1712 bp with an open reading frame (orf) of 1302 bp encoding 433 amino acids. the enolase ii cdna sequence was 1549 bp with an orf of 1296 bp encoding 431 amino acids. the amino acid sequences of the two genes share several conserved features/sites of enolase. antheraea pernyi enolase i shows 93%- ... | 2010 | 20923858 |
| the clock gene period of the housefly, musca domestica, rescues behavioral rhythmicity in drosophila melanogaster. evidence for intermolecular coevolution? | in drosophila, the clock gene period (per), is an integral component of the circadian clock and acts via a negative autoregulatory feedback loop. comparative analyses of per genes in insects and mammals have revealed that they may function in similar ways. however in the giant silkmoth, antheraea pernyi, per expression and that of the partner gene, tim, is not consistent with the negative feedback role. as an initial step in developing an alternative dipteran model to drosophila, we have identif ... | 2000 | 10655226 |
| sex-linked period genes in the silkmoth, antheraea pernyi: implications for circadian clock regulation and the evolution of sex chromosomes. | regulation of the period (per) gene is a critical feature of circadian clock function in insects. here, we show that per is sex-linked in the silkmoth, antheraea per-nyi. the previously described silkmoth per gene is found on the z chromosome. silkmoth per is not dosage compensated at either the rna or the protein level. although earlier studies showed the presence of an oscillating endogenous antisense per transcript, we show that this transcript comes from a locus on the female-specific w chro ... | 1999 | 10624958 |
| molecular genetic relationships between bombycidae and saturniidae based on the mitochondria dna encoding of large and small rrna. | the phylogenetic relationships between bombycidae (bombyx mori and bombyx mandarina) and saturniidae (antheraea yamamai and antheraea pernyi) were investigated based on large and small mitochondiral rrna genes. about 430 bp of four kinds of pcr-amplified fragments were sequenced and aligned. for the 16s rrna gene, b. mori shared a 98, 87 and 86% sequence homology with b. mandarina, a. yamamai and a. pernyi, and for the 12s rrna gene, b. mori shared a 99, 89 and 88% sequence homology with b. mand ... | 1999 | 10609758 |
| ecdysone-initiated developmental changes in wing epidermis rna polymerase activity during the adult development of the oak silkmoth, antheraea pernyi. | | 1978 | 720744 |
| the comparative molecular study between bombycidae and saturniidae based on mtdna rflp and cytochrome oxidase i gene sequences: implication for molecular evolution. | the phylogenetic relationships between bombyx mori and bombyx mandarina species of bombycidae, and antheraea yamamai and antheraea pernyi species of saturniidae were investigated based on mtdna rflp and cytochrome oxidase i gene. the sizes of the mtdna of all the species were estimated at approximately 16 kbp +/- 500 bp by total length of all the restricted fragments and no variation in size was recognized. of the fourteen different restriction endonucleases used, bamhi, hindiii, psti, ecori and ... | 2009 | 10488569 |
| age-related changes in the dendrites of olfactory receptor neurons in the male silkmoth antheraea peryni (insecta, lepidoptera: saturniidae). | a study was conducted to evaluate the changes that occur with aging at the dendrite level of the olfactory receptor neuron in the male silkmoth antheraea pernyi. using calcein am/ethidium homodimer-1 solutions, we found increased numbers of dendrites with damaged membrane with aging. correspondingly there was an overall decrease in the electrophysiological activity as evidenced by the decreased number of cells discharging nerve impulse in response to female pheromone. it was also seen that the n ... | 1998 | 10328853 |
| dna sequence organization in the lepidopteran antheraea pernyi. | a large fraction of the genome of the lepidopteran antheraea pernyi consists of interspersed single-copy sequences and repetitive dna sequences of 300 nucleotide average length. most of the single-copy sequences are about 800 nucleotides long and a minority are considerably longer. thus, the organization of the dna sequences of this insect is similar to that of most higher organisms and different from that of drosophila. | 1976 | 1065878 |
| [ultrastructure and cytochemistry of hemocytes of antheraea pernyi guer. (lepidoptera, attacidae) during the fifth larval stage. i. prohemocytes, plasmatocytes and granulocytes]. | | 1976 | 1271512 |
| respiratory enzymes of the silkmoth antheraea pernyi in relation to metamorphosis and pupal diapause. | | 1974 | 4154824 |
| temperature sensitive programming of the silkmoth flight clock: a mechanism for adapting to the seasons. | when males of the silkmoth antheraea pernyi were exposed to 12 degrees c diuring adult development and then tested for flight activity at 25 degrees c, the time of the onset of the activity was advanced to the early part of the night. this change in the time of activity was stable and persisted through the life of the moth. females showed a corresponding shift in the time of sex pheromone release when they were treated in the same fashion. | 1973 | 17817964 |
| brain control of embryonic circadian rhythms in the silkmoth antheraea pernyi. | the clock protein per is necessary for circadian control of egg-hatching behavior in the silkmoth antheraea pernyi. since the brain and midgut of the silkmoth embryo contain per-positive cells, we examined the circadian clock potential of these embryonic tissues. transplantation experiments indicate that the circadian clock controlling egg-hatching behavior resides in brain, and that a humoral factor mediates this circadian regulation. we also used ligation experiments on first instar larvae to ... | 1998 | 9581765 |
| preferential codon usage and two types of repetitive motifs in the fibroin gene of the chinese oak silkworm, antheraea pernyi. | in this paper we describe the peculiar structures and preferential codon usage found in wild silkworm fibroin genes. we determined a 1350 bp nucleotide sequence from the chinese oak silkworm, antheraea pernyi. the deduced amino acid sequence was partitioned into thirteen polyalanine-containing repetitive motifs, which was one of the characteristics of antheraea fibroins. eleven of these arrays can be classified into two types of motifs depending on difference in amino acid sequences following po ... | 1997 | 9013260 |
| hour-glass behavior of the circadian clock controlling eclosion of the silkmoth antheraea pernyi. | the emergence of the pernyi silkmoth from the pupal exuviae is dictated by a brain-centered, photosensitive clock. in continuous darkness the clock displays a persistent free-running rhythm. in photoperiod regimens the interaction of the clock with the daily lightdark cycle produces a characteristic time of eclosion. but, in the majority of regimens (from 23l:1d to 4l:20d), the eclosion clock undergoes a discontinuous "hourglass" behavior. thus, during each daily cycle, the onset of darkness ini ... | 1971 | 5276768 |
| cellular response to ecdysone: rna and protein synthesis in larval tissues of oak silkworm antheraea pernyi. | | 1970 | 5433729 |
| delay and acceleration of breaking diapause of antheraea pernyi and laspeyresia pomonella by photoperiod manipulation. | | 1970 | 5478074 |
| the action spectrum for breaking diapause in the codling moth, laspeyresia pomonella (l.), and the oak silkworm, antheraea pernyi guer. | the action spectrum for breaking diapause in the oak silkworm, antheraea pernyi guer., and the codling moth, laspeyresia pomonella (l.), was determined from 400 to 700 nm with a wedge-interference spectrograph. insects were exposed to ten hours of white light, followed by six hours of spectral light each day for 45 days. the portion of the spectrum between 400 and 500 nm was found to be the most effective in terminating diapause. diapause was broken for both insects with energy levels as low as ... | 1969 | 16578705 |
| [study of nerve development in antheraea pernyi guer. (lep.), with special reference to the dorso-longitudinal flight muscles]. | | 1969 | 5387091 |
| period protein is necessary for circadian control of egg hatching behavior in the silkmoth antheraea pernyi. | we examined the molecular basis of the circadian control of egg hatching behavior in the silkmoth antheraea pernyi. egg hatching is rhythmically gated, persists under constant darkness, and can be entrained by light by midembryogenesis. the time of appearance of photic entrainment by the silkmoth embryo coincides with the appearance of period (per) and timeless (tim) proteins in eight cells in embryonic brain. although daily rhythms in per and/or tim immunoreactivity in embryonic brain were not ... | 1996 | 8938122 |
| molecular characterization of prothoracicotropic hormone (ptth) from the giant silkmoth antheraea pernyi: developmental appearance of ptth-expressing cells and relationship to circadian clock cells in central brain. | using a pcr strategy, we have cloned the cdna for prothoracicotropic hormone (ptth) from the giant silkmoth, antheraea pernyi. the a. pernyi ptth cdna encodes a preprohormone of 221 amino acids that is 51 and 71% identical at the amino acid level with bombyx mori and samia cynthia ricini ptths, respectively. bacterially expressed, recombinant a. pernyi ptth stimulates adult development when injected into debrained pupae. ptth protein (ca. 30 kda by western blot) and mrna (ca. 0.9 kb by northern ... | 1996 | 8812139 |
| period protein from the giant silkmoth antheraea pernyi functions as a circadian clock element in drosophila melanogaster. | homologs of the drosophila clock gene per have recently been cloned in lepidopteran and blattarian insect species. to assess the extent to which clock mechanisms are conserved among phylogenetically distant species, we determined whether per protein from the silkmoth antheraea pernyi can function in the drosophila circadian timing system. when expressed in transgenic drosophila, the silkmoth per protein is detected in the expected neural cell types, with diurnal changes in abundance that are sim ... | 1995 | 7619519 |
| attachment and growth of fibroblast cells on silk fibroin. | the attachment and growth of fibroblast cells (l-929) on matrices of silk fibroin from bombyx mori domestic silkworm (dsf) and antheraea pernyi wild silkworm (wsf) were studied by a cell culture method. the performance of the two kinds of silk fibroin was compared to that of collagen. dsf exhibited as high a cell attachment and growth as collagen did. the cells attached to dsf were extensively spread out and their filopodia were visible in the sem pictures. wsf, which contains the tripeptide seq ... | 1995 | 7695601 |
| [free nucleotides and their derivatives in fat body of larva of the silkworm, antheraea pernyi g]. | | 1967 | 5599804 |
| thymidine kinase and thymidylate kinase in the silkworm, antheraea pernyi. | | 1966 | 5961623 |
| [the state of protocerebral neurosecretory cells on antheraea pernyi guèr. (lepidoptera, attacidae) during caterpillar development state]. | | 1965 | 5877005 |
| [cytochemical investigation of the silk glands in antheraea pernyi]. | | 2004 | 5872148 |
| a study of spontaneous electrical activity and localization of cholinesterase in the nerve ganglia of antheraea pernyi guer. at different stages of metamorphosis and in pupal diapause. | | 1965 | 5828292 |
| an electron microscope study of the stretch receptor of antheraea pernyi (lepidoptera, saturniidae). | | 1965 | 5827532 |
| rna metabolism in pupae of the oak silkworm, antheraea pernyi: the effects of diapause, development, and injury. | | 1964 | 16591247 |
| enzymatic mechanism for the escape of certain moths from their cocoons. | an "escape hatch" from the cocoon of antheraea pernyi is established by the hydrolytic action of a virtually pure proteinase which the moth first secretes and then dissolves by means of a solvent which maintains the enzyme at optimum ph. the proteinase is both synthesized and secreted by the maxillary galeae. the solvent derives from a pair of labial glands which have the form of long convoluted tubules lying alongside the foregut and opening to the exterior through a single aperture in the head ... | 1964 | 17806809 |
| photoperiodic control of pupal diapause in the silkworm, antheraea pernyi. | | 1964 | 17836396 |
| [trehalose in the organism of antheraea pernyi in various stages of its development]. | | 2007 | 13889469 |
| cloning of a structural and functional homolog of the circadian clock gene period from the giant silkmoth antheraea pernyi. | the period (per) gene of drosophila plays an important role in circadian clock function. interestingly, homologs of per have not been cloned outside of dipteran species. using a pcr strategy, we now report the cloning of the cdna of a per homolog from the silkmoth antheraea pernyi. the cdna encodes a protein of 849 amino acids, which shows highest identity (39%) with the per protein of drosophila virilis. stretches of high identity between moth and fly proteins are in the amino terminus, the pas ... | 1994 | 7946353 |
| expression of a pheromone-binding protein in insect cells using a baculovirus vector. | a cdna encoding a pheromone-binding protein from the male silkmoth antheraea pernyi has been integrated into the genome of the autographa californica multiple nuclear polyhydrosis virus such that the transcription was under the control of the strong polyhedrin promoter. recombinant pheromone-binding protein was expressed in a baculovirus-infected insect cell line (sf9) and secreted from the cells into the culture medium. using a two-step protocol, recombinant pheromone-binding protein has been i ... | 1992 | 1730221 |
| [change of the amino acid composition of tissue proteins during the development of antheraea pernyi larvae]. | | 2016 | 13699494 |
| [metabolic relationship between carbohydrates and nitrogen in antheraea pernyi pupa during metamorphosis]. | | 2016 | 13628784 |
| nucleic acids and other phosphorus fractions in the course of metamorphosis of the chinese tussur moth (antheraea pernyi). | | 1958 | 13532259 |
| [fats in the organism of antheraea pernyi]. | | 2007 | 13445768 |