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temporal association of amblyomma americanum with the presence of ehrlichia chaffeensis reactive antibodies in white-tailed deer.from 1981 through 1993, tick infestations and serum antibodies reactive to ehrlichia chaffeensis, the causative agent of human ehrlichiosis, were monitored among white-tailed deer (odocoileus virginianus) at whitehall experimental forest, clarke county, georgia (usa). neither ticks nor e. chaffeensis antibodies were detected during the first two years of the study. infestations of the lone star tick (amblyomma americanum), a suspected vector of e. chaffeensis, first were noted on deer in 1983. p ...19958583627
hemocytic rickettsia-like organisms in ticks: serologic reactivity with antisera to ehrlichiae and detection of dna of agent of human granulocytic ehrlichiosis by pcr.ixodid ticks were collected from connecticut, massachusetts, missouri, pennsylvania, rhode island, and british columbia (canada) during 1991 to 1994 to determine the prevalence of infection with hemocytic (blood cell), rickettsia-like organisms. hemolymph obtained from these ticks was analyzed by direct and indirect fluorescent antibody (fa) staining methods with dog, horse, or human sera containing antibodies to ehrlichia canis, ehrlichia equi, or rickettsia rickettsii. of the 693 nymphal and a ...19958567911
ixodid ticks from panthers and bobcats in florida.ixodid ticks were present in all 189 samples examined from 53 florida panthers (felis concolor coryi, 104 collections) and 85 bobcats (felis rufus floridana) in florida (usa) between 1974 and 1991. we identified 3,251 ticks from panthers and 918 from bobcats. specimens of dermacentor variabilis, ixodes scapularis, i. affinis, amblyomma maculatum, and a. americanum were present on 49, 39, 17, seven, and two of the 53 florida panthers, respectively, and comprised 36%, 55%, 7%, 1%, and < 1% of the ...19958592378
uptake, incorporation and redistribution of arachidonic acid in isolated salivary glands of the lone star tick.the ability of isolated salivary glands from the lone star tick, amblyomma americanum, to take up, incorporate and redistribute [3h]arachidonic acid was examined. uptake of arachidonic acid was concentration dependent--a single salivary gland incorporated up to approximately 2.8 micrograms arachidonic acid in 60 min. over 90% of the [3h]arachidonate entering the glands was esterified and found only in the phospholipid (approximately 80%) and triglyceride (approximately 10%). essentially no radio ...19957742831
field evaluation of deet and a piperidine repellent (ai3-37220) against amblyomma americanum (acari: ixodidae).repellent efficacy of deet (n,n-diethyl-3-methylbenzamide) and a piperidine, ai3-37220, was evaluated topically on human volunteers against lone star tick, amblyomma americanum (l.), nymphs and adults in the field. ai3-37220, at 0.5 mg/cm2, provided > 90% repellency against adult and nymphal ticks over a 6-h test period and showed significantly better repellent efficacy than deet. deet, at the same concentration, provided 85% repellency at 0 h and deteriorated to 55% repellency at 6 h.19958551512
regulation of free arachidonic acid levels in isolated salivary glands from the lone star tick: a role for dopamine.an important regulatory step for prostaglandin synthesis is the availability of the precursor, free arachidonic acid (aa). in isolated salivary glands of the lone star tick, amblyomma americanum (l.), the level of free aa appears to depend on higher phospholipase a2 (pla2) activity rather than decreased rates of re-esterification by lysophosphatide acyl transferase (lat). this conclusion is supported by experiments where inhibition of lat with merthiolate was without effect, while the calcium io ...19957655056
integrated management strategies for amblyomma americanum (acari: ixodidae) on pastured beef cattle.data on tick control and knowledge of the damage caused to beef cattle by tick feeding were incorporated into a computerized dynamic life table model (lstsim) for amblyomma americanum (l.). simulations were made to determine economically feasible, 5-yr integrated pest management (ipm) strategies for a. americanum in forage areas utilized by bos taurus, bos indicus, and crossbred cattle (b. taurus x b. indicus). the effects of host resistance, pasture rotation, habitat conversion, topical acarici ...19947932604
evidence supporting the presence of borrelia burgdorferi in missouri.although lyme disease is commonly seen in the southcentral united states, the epidemiology of the disease is poorly defined there. the purpose of this study was to document the presence of borrelia burgdorferi in ticks collected in southeastern missouri and around the city of st. louis. spirochetes were detected and identified as b. burgdorferi by immunofluorescent antibody (ifa) tests using the monoclonal antibody h5332 in 1.9% of amblyomma americanum and 2.0% of dermacentor variabilis ticks co ...19947943575
origin, accumulation and mobilization of salivary prostaglandin precursors in the lone star tick. 19947958372
ticks (acari: ixodidae) collected from small and medium-sized kansas mammals.seven species of hard-bodied ticks were collected from 20 species of small and medium-sized mammals in kansas; amblyomma americanum l., dermacentor variabilis (say), haemaphysalis leporispalustris (packard), ixodes cookei packard, i. kingi bishopp, i. sculptus neumann, and i. texanus banks. dermacentor variabilis was found statewide, a. americanum only in the eastern one-third of the state, and the ixodes spp. and h. leporispalustris were widely scattered. the most common tick found was d. varia ...19948057327
distribution of arachidonic acid among phospholipid subclasses of lone star tick salivary glands.the subclass composition of choline- and ethanolamine-containing phospholipids was determined by analysis of acyl-linked fatty acids released by base hydrolysis of diradylglycerobenzoates formed from lone star tick salivary gland diacyl, alkylacyl and alkenylacyl phospholipids. the diacyl subclass comprises 87% of all choline-containing phospholipids, while th alkylacyl subclass comprises c. 9% and the alkenylacyl subclass c. 4%. the diacyl subclass comprises 72-77% of ethanolamine-containing ph ...19948069331
genome size and organization in the ixodid tick amblyomma americanum (l.).we used dna reassociation kinetics to determine genome size and organization in the ixodid tick amblyomma americanum. we calculated the genome size of a. americanum to be approximately 1.08 pg or 1.04 x 10(9) base pairs and to consist of 35.8% unique dna, 4.2% foldback sequences, 17.9% highly repetitive sequences, and 42.1% moderately repetitive sequences. comparison of the reassociation kinetics of long and short fragments revealed repetitive sequences to be distributed in a pattern of long per ...19948069417
ectoparasites of opossums and raccoons in southeastern georgia.twelve species of ectoparasites (four fleas, three ticks, three chiggers, one macronyssid mite, and one atopomelid mite) were recovered from 42 opossums, didelphis virginiana kerr, live-trapped from september 1992 through april 1994 in southeastern georgia. the fleas, ctenocephalides felis (bouché) (prevalence = 19%) and polygenis gwyni (c. fox) (36%); the ticks, dermacentor variabilis (say) (40%) and ixodes scapularis say (19%); and the macronyssid mite, ornithonyssus wernecki (fonseca) (21%), ...19947815408
serologic diagnosis of human monocytic ehrlichiosis by immunoblot analysis.human monocytic ehrlichiosis is caused by ehrlichia chaffeensis, an intracellular bacterium probably transmitted by the tick amblyomma americanum in the united states. despite its lack of specificity in discriminating among infections by closely related ehrlichia spp., immunofluorescence assay (ifa) is the most frequently used serological diagnostic method. to improve the specificity of the serological diagnosis, we compared antigenic profile of e. canis and e. chaffeensis antigen with homologou ...19948556515
seasonal and annual abundance of amblyomma americanum (acari: ixodidae) in central georgia.seasonal and annual changes in the abundance of amblyomma americanum (l.) larvae, nymphs, and adults were monitored over a 5-yr period in the piedmont physiographic region of georgia. tick abundance was monitored with cloth drags (all life stages) and co2-baited cloth panels (nymphs and adults) monthly during march through september from 1987 through 1991. larvae appeared in substantial numbers in july and were most numerous in august and september. small numbers of unfed larvae apparently overw ...19948158632
influence of annual and biennial prescribed burning during march on the abundance of amblyomma americanum (acari: ixodidae) in central georgia.a 5-yr study to evaluate the influence of annual and biennial prescribed burning in march on the abundance of amblyomma americanum (l.) was conducted on oconee wildlife management area in the piedmont physiographic region of georgia. tick abundance on plots embedded in larger burned and unburned areas were monitored with cloth drags and co2-baited cloth panels monthly during march through september from 1987 through 1991. annual and biennial prescribed burning from 1988 to 1991 significantly red ...19948158633
prevalence of borrelia (spirochaetaceae) spirochetes in texas ticks.between 1990 and 1992, ticks from eight texas parks were collected and analyzed to determine the prevalence of spirochete-infected ticks. borrelia spirochetes were detected in 1.03% of 5,141 amblyomma americanum (l.) adults examined, a species texas residents often encounter. no spirochetes were observed in the other tick species tested.19948189421
new version of lstsim for computer simulation of amblyomma americanum (acari: ixodidae) population dynamics.a previous version of lone star tick simulation model (lstsim) for a wildlife ecosystem was revised and expanded to include a beef cattle forage area and improved handling of tick-host-habitat interactions. relationships between environmental and biological variables were also refined in the new version. general validity of the revised model was established by comparing simulated and observed host-seeking populations of amblyomma americanum (l.) at five geographic locations, three in oklahoma an ...19938254630
changes in lipids of the salivary glands of the lone star tick, amblyomma americanum, during feeding.the lipid composition of salivary glands from male and female lone star ticks, amblyomma americanum, was investigated at progressive stages of tick feeding. the amounts of fatty acids from both phospholipid and neutral lipid fractions increased dramatically during the initial stage of feeding and peaked in partially fed females weighing 100-250 mg. percentage compositions of myristic (14:0) and palmitic acid (16:0) decreased, but stearic (18:0), oleic (18:1), linoleic (18:2), and arachidonic aci ...19938277375
analysis of lipids in the salivary glands of amblyomma americanum (l.): detection of a high level of arachidonic acid.analysis of lipids in salivary glands of the lone star tick, amblyomma americanum, demonstrated that arachidonic acid (20:4, n-6) comprises 8% of all fatty acids identified by gas chromatography. the occurrence of arachidonic acid and other c20 polyunsaturated fatty acids in tick salivary glands was confirmed by gas chromatography-mass spectrometry. arachidonate is located entirely in the phospholipid fraction and is associated exclusively with phosphatidylcholine (pc) and phosphatidylethanolami ...19938324244
implications of selection and hybridization studies on the mode of inheritance of photoperiodically induced developmental diapause in laboratory strains of the lone star tick (acari: ixodidae).selection of lone star ticks, amblyomma americanum (l.), that were reared in the laboratory under short-day photoperiods of 10:14 (l:d) h resulted in third generation (f3) diapause versus f3 nondiapause strain replete nymphs having significantly different mean molting times (days from detachment to ecdysis) of 100.9 versus 54.7 d, respectively; whereas, the f7 unselected laboratory control strain had an even smaller, significantly different mean molting time of 49.5 d. two hybrid crosses of f2 n ...19938433316
amblyomma americanum: a potential vector of human ehrlichiosis.polymerase chain reaction primers specific for ehrlichia chaffeensis were used to amplify dna from extracts of pooled ticks. amplification was performed on extracts from 140 pools (1,579 total ticks) consisting of three tick genera collected from five states. the characteristic 389-basepair product was observed after amplification of extracts from seven different pools of adult amblyomma americanum (117 pools, 1,462 ticks), but not from pools of nymphs. no specific product was observed after amp ...19938357086
computer-controlled olfactometer system for studying behavioral responses of ticks to carbon dioxide.an olfactometer system that consisted of a laptop computer, a carbon dioxide analyzer, and an apparatus to control gas flows was designed to study ixodid tick behavioral responses to varying carbon dioxide (co2) concentrations. the system provided a method to regulate and measure stimulatory co2 concentrations (1-99 ppm) above ambient co2 background levels. adult amblyomma americanum (l.) and dermacentor variabilis (say) demonstrated behavioral responses to co2 concentrations as low as 9 ppm abo ...19938510116
ticks (acari: ixodidae) infesting medium-sized wild mammals in southwestern tennessee.seven species of ixodid ticks (n = 2,661) were found on medium-sized wild mammals (n = 295) during a 13-mo study in shelby county, tennessee. the seven tick species collected were dermacentor variabilis (say), amblyomma americanum (l.), rhipicephalus sanguineus (latreille), ixodes texanus (banks), i. cookei (packard), i. scapularis (say), and haemaphysalis leporispalustris (packard). the raccoon had the greatest tick species diversity in all of the aforementioned ticks except h. leporispalustris ...19938254637
isolation and transmission of the lyme disease spirochete from the southeastern united states.the isolation of the lyme disease spirochete (borrelia burgdorferi) from the southeastern united states is reported. three isolates, two from cotton mice (peromyscus gossypinus) and one from the black-legged tick (ixodes scapularis), were recovered from sapelo island, georgia, in july and september 1991. the spirochetes were characterized by indirect fluorescent antibody assay using a battery of five monoclonal antibodies, by sodium dodecyl sulfate/polyacrylamide gel electrophoresis (sds/page) o ...19938346258
monthly incidence of theileria cervi and seroconversion to babesia odocoilei in white-tailed deer (odocoileus virginianus) in texas.monthly monitoring of fawns collected from an area in texas endemic for theileria cervi and babesia odocoilei showed that transmission of t. cervi occurred during july and august, a time period consistent with the occurrence of amblyomma americanum. seroconversion to b. odocoilei occurred during october to december and possibly continued through january and february. the time of seroconversion was more suggestive of transmission of b. odocoilei by ixodes scapularis than by amblyomma americanum.19921512881
borrelia sp. in ticks recovered from white-tailed deer in alabama.six hundred sixty-five hunter-killed white-tailed deer (odocoileus virginianus) from 18 counties in alabama (usa) were examined for ticks. most of the collections were made at state-operated wildlife management areas. four species of ticks (n = 4,527) were recovered: the lone star tick amblyomma americanum (n = 482); the gulf coast tick a. maculatum (n = 11); the winter tick dermacentor albipictus (n = 1,242); and the black-legged tick ixodes scapularis (n = 2,792). fifty-six percent of the tick ...19921512879
attempted transmission of borrelia burgdorferi (spirochaetales: spirochaetaceae) (jdi strain) by ixodes scapularis (acari: ixodidae), dermacentor variabilis, and amblyomma americanum.laboratory-reared ixodes scapularis say, amblyomma americanum (l.), and dermacentor variabilis (say) were fed on new zealand white rabbits experimentally infected with borrelia burgdorferi (jdi strain). at repletion, spirochetes could be detected by dark-field microscopy only in i. scapularis. acquisition rates were 18 and 21%. when previously exposed nymphs of each species were fed on susceptible rabbits, i. scapularis was the only tick of the three species that transmitted b. burgdorferi. when ...19921495078
field evaluation of two formulations of cyfluthrin for control of ixodes dammini and amblyomma americanum (acari: ixodidae).liquid or granular cyfluthrin was applied in the spring and fall to different test plots at a rate of 0.41 (ai) kg/ha to control ixodes dammini spielman, clifford, piesman & corwin and amblyomma americanum (l.). spring application of liquid cyfluthrin showed the most significant decreases of both species. i. dammini nymphs were reduced 97% at 10 d, 100% at 2 mo, and 100% at 1 yr; and a. americanum adults and nymphs were reduced 91-93% at 10 d and 100% at 2 mo posttreatment. granular cyfluthrin a ...19921495073
parasites of cottontail rabbits of southern illinois.fifteen species of parasites including haemaphysalis leporispalustris, ixodes dentatus, amblyomma americanum, cediopsylla simplex, odontopsyllus multispinosus, cuterebra sp., obeliscoides cuniculi, trichostrongylus calcaratus, trichostrongylus affinis, longistriata noviberiae, dermatoxys veligera, trichuris sp., mosgovoyia sp., taenia pisiformis, and hasstilesia tricolor as well as coccidia oocysts were collected from 96 cottontail rabbits (sylvilagus floridanus) confined to a pen in southern il ...19921491303
synganglial morphology and neurosecretory centers of adult amblyomma americanum (l.) (acari: ixodidae).lone star ticks, amblyomma americanum (l.), were processed by standard histological means for paraffin embedding, sectioning, and staining by the paraldehyde-fuchsin technique. the synganglion is highly condensed around the esophagus and possesses paired optic, cheliceral, palpal, pedal i-iv nerves, and opisthosomal nerves and a single unpaired esophageal nerve. although optic nerves were observed leading from the eyes to the protocerebrum, distinct optic ganglia were not seen in any of the prep ...19921460618
oral secretion elicited by effectors of signal transduction pathways in the salivary glands of amblyomma americanum (acari: ixodidae).the abilities of various effectors of signal pathways in tick salivary glands to stimulate oral secretions in partially fed amblyomma americanum (l.) female ticks were compared. pilocarpine stimulated secretion the most rapidly following tick stimulation, but its effectiveness declined with time and subsequent injections. secretion rates induced by dopamine and theophylline increased with time of collection and additional injections and were as effective as pilocarpine after 60 min. activators o ...19921552527
tick sweep: modification of the tick drag-flag method for sampling nymphs of the deer tick (acari: ixodidae).we describe a version of the standard tick drag-flag modified for use in close-growing and tangled vegetation, as well as under ornamental shrubbery and fallen branches. two major features of the sweep are: (1) it allows the user to remain upright with the flag parallel to the ground, thus sampling effectively beneath low and fallen branches and around shrubs, as well as capturing host-seeking ticks in advance of the operator; and (2) the use of a flannel rubberized-laminate fabric (crib sheet) ...19921495058
tick (acari: ixodidae) attachment cement and salivary gland cells contain similar immunoreactive polypeptides.a specific antiserum (12c) raised to a 90-kda immunogenic component of salivary glands of the tick rhipicephalus appendiculatus recognized similar 90-kda polypeptides from salivary glands of the american dog tick, dermacentor variabilis, and the lone star tick, amblyomma americanum, as well as 70-kda polypeptides in the cement of d. variabilis, a. americanum, and r. sanguineus (brown dog tick). the reduction in size of the polypeptide for these ticks suggests that it is modified in some way duri ...19921495047
ectoparasites of the blackbuck antelope (antilope cervicapra).fifty-two free-ranging blackbuck antelope (antilope cervicapra) from texas were examined for ectoparasites. two species of sucking lice (anoplura), one species of chewing louse (mallophaga), one species of louse fly (diptera), and three species of ticks (acari) were found. this is the first report of the anoplurans linognathus cervicaprae and l. pithodes from the western hemisphere. the southern deer ked (lipoptena mazamae), the winter tick (dermacentor albipictus), and the rabbit tick (haemaphy ...19921512888
distribution, density, and lyme disease spirochete infection in ixodes dammini (acari: ixodidae) on white-tailed deer in maryland.a statewide survey of ticks parasitizing white-tailed deer was carried out in maryland during november 1989 to assess the status of the deer tick, ixodes dammini spielman, clifford, piesman & corwin, the major vector of lyme disease in the northeastern united states. ticks were collected from deer carcasses brought in by hunters at 23 check stations (one per county). a total of 3,437 i. dammini were collected from 538 of 1,281 deer (42%), together with 2,013 dermacentor albipictus (packard) and ...19921552529
amblyomma americanum (l.): protein kinase c-independent fluid secretion by isolated salivary glands.protein kinase c activity was partially purified from tick salivary glands by fast protein liquid chromatography anion-exchange chromatography. enzyme activity was stimulated by ca2+, phosphatidylserine, and diacylglycerol with the highest activity observed in the presence of all three modulators. enzyme activity was inhibited by a synthetic pseudosubstrate peptide with an amino acid sequence resembling the protein kinase c substrate phosphorylation site. the protein kinase c activator, 1-oleoyl ...19921582485
quantitative evaluation of sampling methods for ixodes dammini and amblyomma americanum (acari: ixodidae).three tick-sampling methods (dry ice-baited tick traps, cloth drags, and ambulatory human host) were evaluated to determine which technique yielded the greatest capture of host-seeking stages of ixodes dammini spielman, clifford, piesman & corwin and amblyomma americanum (l.). the most reliable method, catching more stages and significantly more numbers of i. dammini and a. americanum; was dry ice-baited tick traps. there were no significant differences between the drag and human-host methods fo ...19921625293
amblyomma americanum: characterization of salivary prostaglandins e2 and f2 alpha by rp-hplc/bioassay and gas chromatography-mass spectrometry.secretagogue-induced saliva of the tick, amblyomma americanum (l.) was fractionated by reversed-phase-high-performance liquid chromatography (rp-hplc) and bioassayed in smooth muscle preparations. material with retention times of authentic prostaglandin e2 (pge2) and prostaglandin f2 alpha (pgf2 alpha) were found to cause contraction of preparations of rat colon and rat stomach strips. gas chromatography-mass spectra of selected ions of both hplc-purified fractions confirmed the existence of pge ...19921730268
inability of ixodes cookei and amblyomma americanum nymphs (acari: ixodidae) to transmit borrelia burgdorferi.the vector competency of ixodes cookei packard and amblyomma americanum (l.) for borrelia burgdorferi was studied using syrian hamsters. ixodes dammini spielman, clifford, piesman & corwin were used as controls. darkfield and immunofluorescent examinations of midgut diverticula revealed b. burgdorferi spirochetes in 32 of 36 (88.9%) i. dammini larvae, 5 of 36 (13.9%) i. cookei larvae, and 7 of 36 (19.4%) a. americanum larvae within 48 h after feeding on infected syrian hamsters. b. burgdorferi w ...19921625302
horizontal movement of adult ixodes dammini (acari: ixodidae) attracted to co2-baited traps.the ability of male and female deer ticks, ixodes dammini spielman, clifford, piesman, & corwin, to move horizontally was evaluated by performing a mark-recapture study. in each of two experiments, 120 ticks were marked with fluorescent powder and released at predetermined distances from a co2-baited trap. the mean recruitment distance was 1.8 m after 6 d of trap operation. this distance is appreciably lower than those calculated from previous studies of several other species of ixodid ticks, in ...19911941943
tick infestations of white-tailed deer in alabama.four species of ticks were collected from 537 white-tailed deer (odocoileus virginianus), examined during the hunting seasons (november to january) of 1988-89 and 1989-90 at selected locations in alabama (usa). ixodes scapularis was the most common tick recovered (2,060 specimens) and infested 54% of the deer. dermacentor albipictus was the second most frequent tick (1,253 specimens) and infested 15% of the deer. amblyomma americanum was the third most frequent tick (315 specimens) and infested ...19911758026
role of genital sex pheromones in amblyomma americanum and a. maculatum (acari: ixodidae).amblyomma americanum and a. maculatum were studied to determine if genital sex pheromones were present, as reported in dermacentor species. chemical analysis of methanol extracts of the anterior reproductive tracts (source of the genital sex pheromone) of a. americanum and a. maculatum confirmed the presence of the same fatty acids that act as components of the genital sex pheromone in dermacentor variabilis and d. andersoni. in both amblyomma species, removal of the anterior reproductive-tract ...19911860418
effect of ivermectin on the volume of blood ingested by two species of ticks (acari: ixodidae) feeding on cattle.females of the lone star tick, amblyomma americanum (l.), and the american dog tick, dermacentor variabilis (say), were fed on ivermectin (ivomec)-treated and untreated bovines to determine the effect of the acaricide on volume of blood ingested and to compare the weight differences between the treatment and control groups at various time intervals after attachment. adult females from each genus were collected from bos tarus hosts and subjected to hematin assays on three collection dates to esti ...19911875376
manifestations of immunity in sheep repeatedly infested with amblyomma americanum ticks.three sheep were infested 4 times with 100 amblyomma americanum tick pairs and kept indoors until the natural termination of the infestations. characteristics of the tick populations that show efficiency of feeding, fertility, and offspring development, and elisa antibodies to tick salivary gland extracts were studied at each infestation. on average, female ticks fed on tick-naive sheep detached at 12.1 +/- 0.2 (mean +/- standard error) days, weighed 492 +/- 16.8 mg, engorged 40.7 mg per day, an ...19911919917
antigens of amblyomma americanum ticks recognized by repeatedly infested sheep.sera were taken from 3 sheep that had been infested 5 times with amblyomma americanum and that exhibited manifestations of humoral depression to homologous antigens and anti-tick resistance. proteins extracted from the intestine or salivary glands of unfed ticks or salivary glands from partially (3-day) fed ticks were analyzed by polyacrylamide gel electrophoresis (page) and sodium dodecyl sulfate-page. antigens recognized by the sheep in the same materials before and after each infestation were ...19911919918
selective breeding for photoperiodically induced developmental diapause in laboratory strains of amblyomma americanum (acari: ixodidae).offspring of amblyomma americanum (l.) reared for four generations in a 12:12 (l:d) photoperiod in the laboratory were held in 10:14 (l:d) conditions and bred to select for developmental diapause (dia) or nondiapause (non) characteristics for two additional generations. sequential generations of fed dia nymphs had mean molting times (days from detachment to ecdysis) that were 8.0% (dia1) and 35.7% (dia2) greater than parental ticks, and maximal development time was extended by 20% in dia2 ticks ...19911941917
mechanisms of host-tick contact with special reference to amblyomma americanum (acari: ixodidae) in beef cattle forage areas.at least five groups of parameters regulate contact between ticks and host animals. group 1 (seasonal effects) and group 2 (daily effects) comprise abiotic parameters and include daylength, temperature, relative humidity, and interactions thereof. groups 3, 4, and 5 consist of biotic parameters. these pertain to biological processes in ticks (group 3), such as hunger responses and spatial patterns in aphagous tick populations; biological processes in host animals (group 4), such as patterns of h ...19911941920
etiologic agent of lyme disease, borrelia burgdorferi, detected in ticks (acari: ixodidae) collected at a focus in alabama.the study was conducted at sites of known transmission of borrelia burgdorferi in east central alabama. the objectives were to determine species of ticks present at these sites, their host associations, and species of ticks and small mammals naturally infected with b. burgdorferi. a total of 451 hosts were examined for ticks, including cotton mice, peromyscus gossypinus (le conte); cotton rats, sigmodon hispidus say & ord; southern short-tailed shrews, blarina carolinensis (bachman); house mice, ...19911941933
borrelia burgdorferi in ticks (acari: ixodidae) from coastal virginia.ixodid ticks removed from hosts and from vegetation during march-november 1987 at sites in coastal virginia and north carolina were examined for borrelia burgdorferi. b. burgdorferi was evident in nine (22%) ixodes cookei packard removed from rice rats (oryzomys palustris), a white-footed mouse (peromyscus leucopus), and raccoons (procyon lotor); four (6%) amblyomma americanum (l.) removed from raccoons; and two (3%) dermacentor variabilis (say) removed from a raccoon and a rice rat. b. burgdorf ...19911941936
ehrlichiosis in children.tick-borne rickettsiae of the genus ehrlichia have recently been recognized as a cause of human illness in the united states. in the years 1986-1988, 10 cases of ehrlichiosis were diagnosed in children in oklahoma. fever and headache were universal: myalgias, nausea, vomiting, and anorexia were also common. rash was observed in six patients but was a prominent finding in only one. leukopenia, lymphopenia, and thrombocytopenia were common laboratory abnormalities. six patients were treated with t ...19911987531
tick information sheet. the lone star tick. amblyomma americanum. 19912014616
increased population densities of amblyomma americanum (acari: ixodidae) on long island, new york.lone star ticks, amblyomma americanum comprised a significantly greater proportion of total ticks flagged on eastern long island and fire island, new york, in 1986 and 1990 than in samples reported by other authors from the 1940s (when a. americanum was not collected by flagging or from hosts) and the 1970s. therefore, population densities of a. americanum apparently have increased in recent years on southeastern long island, where this species now is distributed widely.19912040963
further investigations on the action of ecdysteroids on the salivary glands of the female tick amblyomma americanum.two phytecdysteroids (abutasterone, makisterone a) and five synthetic ecdysteroid analogues, all at 1 microgram/ml, were tested on salivary glands from the female tick, amblyomma americanum l. (acari:ixodidae), held in organ culture for four days. all of these substances caused a significant reduction in fluid secretory competence of salivary glands in vitro. this constitutes further evidence that the structural requirements for causing salivary-gland degeneration in ticks are similar to those g ...19912044433
isolation of borrelia burgdorferi from arthropods collected in texas.the texas department of health laboratory cultured arthropods from november 1988 through december 1989 in an attempt to isolate borrelia burgdorferi, the etiologic agent of lyme disease. spirochetes were isolated from eight of 1,093 pools of arthropods cultured. the spirochetal isolates were from several tick and one flea species, including amblyomma americanum, a. maculatum, ixodes scapularis, and ctenocephalides felis. these 8 isolates reacted specifically when treated with monoclonal antibodi ...19912063950
hyperosmotic oral fluid secretion during active water vapour absorption and during desiccation-induced storage-excretion by the unfed female tick amblyomma americanum. 19912061709
rectal infusion and aspiration of material through the guts of ixodid ticks (acari: ixodidae).a technique for inoculating and removing substances via the anus of vector ticks was devised to define features of vector competence precisely. calibrated inocula (greater than 5 nanoliter) containing aqueous dye and polystyrene beads as well as infectious agents were infused into the rectal sacs of ticks using glass microcapillary pipettes placed within the expanded anal orifice. the guts of preadult and adult ticks, ixodes dammini spielman, clifford, piesman & corwin, dermacentor variabilis (s ...19911770516
putative new expression of genes in ixodid tick salivary gland development during feeding.poly(a+) mrna-enriched fractions from salivary glands of partially fed amblyomma americanum female ticks were translated in vitro with a rabbit reticulocyte translation system. translated proteins were labeled with [35s]methionine, separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis, and identified by autoradiography. thirty major identifiable polypeptides with molecular weights ranging from 14 to 136 kda were synthesized from mrna isolated from salivary glands of ticks weighi ...19911710268
effects of sequential infestations of dermacentor albipictus and amblyomma americanum (acari: ixodidae) on overwintering beef cows in west-central texas.infestations of winter ticks, dermacentor albipictus (packard), observed on mature black angus cows, reached peak levels in january and diminished through march. infestations of adult lone star ticks, amblyomma americanum (l.), began in mid-january and increased through may. cows receiving ectrin, taktic, or deltox treatments on 13 december and 21 february experienced significantly less weight loss and entered the subsequent breeding season with higher average body weight than untreated cows. in ...19902388237
attempted transmission of ehrlichia risticii, causative agent of potomac horse fever, by the ticks, dermacentor variabilis, rhipicephalus sanguineus, ixodes scapularis and amblyomma americanum.dermacentor variabilis, rhipicephalus sanguineus, amblyomma americanum, and ixodes scapularis ticks were investigated for their ability to transmit potomac horse fever. larval and nymphal ticks were exposed to ehrlichia risticii by feeding on mice inoculated with the organism. molted exposed ticks were then allowed to feed on susceptible ponies or mice. no evidence of transmission, either clinically or by detection of antibodies to e. risticii in mice or ponies, was observed for any tick species ...19902307070
cattle breed alters reproduction in amblyomma americanum (acari: ixodidae).female amblyomma americanum [l.] that feed on bos taurus cattle weigh significantly (p = 0.05) more (mean = 505.4 mg) than females that feed on b. indicus x b. taurus cattle (mean = 450.7 mg) but not females that feed on b. indicus cattle (mean = 489.7 mg). females that feed on b. taurus cattle lay more eggs (mean = 3492) and produce more larvae (mean = 3243) than females that feed on b. indicus cattle (mean = 3103 eggs; 2925 larvae) or b. indicus x b. taurus cattle (mean = 2961 eggs; 2759 larva ...19902081445
[the taxonomic aspects of the transmission of the causative agent of lyme disease].according to world literature data 17 species of ixodid ticks have been studied for natural infection with the lyme disease agent. analysis of the data on the level of the infection, transovarial and transphase transmission has shown that main biological vectors of borrelia burgdorferi are the species of the subgenus ixodes s. str. - i. ricinus, i. persulcatus (eurasia), i. dammini, i. pacificus (north america). potential vectors are i. scapularis, i. dentatus, amblyomma americanum, dermacentor ...19902259524
comparative efficiency of flagging and carbon dioxide-baited sticky traps for collecting the lone star tick, amblyomma americanum (acarina: ixodidae).collections of amblyomma americanum (l.) from five habitat types showed that significantly more ticks of all life stages could be collected by co2-baited sticky traps than by the standard flagging technique. the effect of habitat type and season significantly influenced the number of ticks collected by both techniques. traps baited with carbon dioxide revealed adult tick activity in several habitats several weeks earlier than did flagging, because flagging failed to efficiently recover ticks unl ...19902231613
comparative field evaluation of permethrin and deet-treated military uniforms for personal protection against ticks (acari).the effectiveness of permethrin-impregnated (pi) versus permethrin-sprayed (ps) military battle dress uniforms was evaluated in protecting soldiers from tick bite. at the same time, an extended-duration formulation of n,n-diethyl-m-toluamide (deet), when applied to clothing only, was evaluated and compared with the permethrin-treated uniforms. testing consisted of exposing subjects, clothed in either untreated or the variously treated uniforms, to field populations of ticks during a series of 30 ...19902231620
geographic occurrence of ixodes scapularis and amblyomma americanum (acari: ixodidae) infesting white-tailed deer in north carolina.a state-wide survey to determine the occurrence and comparative numbers of ticks infecting white-tailed deer (odocoileus virginianus) was conducted in north carolina (usa). one thousand six hundred twenty nine deer were examined in 60 of 100 counties; with the exception of one county in the piedmont region, all tick-infested deer occurred in the coastal plain. ixodes scapularis (46%) and amblyomma americanum (53%) were the most prevalent species encountered and accounted for more than 98% of the ...19902250335
population growth rates for amblyomma americanum (acari: ixodidae) on bos indicus, b. taurus and b. indicus x b. taurus cattle.densities of amblyomma americanum (l.) on bos indicus, b. taurus and b. indicus x b. taurus cattle are compared over a 3-year period, and the growth rate (rate of increase or decrease) of parasitic tick populations on each cattle genotype is estimated. average log10 densities of parasitic a. americanum larvae are significantly (p = 0.05) lower on b. indicus cattle than on b. taurus and b. indicus xb. taurus cattle. average log densities of nymphal and adult ticks on b. taurus cattle are signific ...19902261818
amblyomma americanum: identification of tick salivary gland antigens from unfed and early feeding females with comparisons to ixodes dammini and dermacentor variabilis.salivary gland antigens involved in host resistance to tick feeding by amblyomma americanum (lone star tick) have been identified. gland extracts from unfed and partially fed 12-, 48-, 72-, 96-, and 120-hr females and their corresponding midgut tissues were analyzed by immunoblotting with sera from naturally immune and hyperimmune sheep and rabbits. polypeptides at 90, 75, 58, 45, 33, and 23 kda from the salivary glands of a. americanum females were consistently observed with antibodies from bot ...19902298277
experimental transmission of a granulocytic form of the tribe ehrlichieae by dermacentor variabilis and amblyomma americanum to dogs.transstadial transmission of granulocytic ehrlichieae in dogs was attempted using ticks, amblyomma americanum and dermacentor variabilis. ticks were exposed by feeding as nymphs on acutely infected pups; adult ticks then fed to repletion on susceptible adult dogs that were monitored daily for signs of infection. evidence of transmission was not observed in control dogs or in those exposed to d variabilis. in contrast, dogs exposed to a americanum developed serologic or clinical evidence of infec ...19902368951
rickettsia in texas.since the first reported case in 1941, rocky mountain spotted fever in humans has been reported from many areas of texas, with two major foci, one located in the north-central region and the other in the eastern region of the state. during the period 1979-1988, 421 cases of rmsf were reported, reaching 108 cases in 1983 and declining in subsequent years. statewide surveillance programs to detect spotted fever group rickettsiae in tick populations were initiated in 1976. in recent years, the sfg ...19902378448
ectoparasitic and phoretic arthropods of virginia opossums (didelphis virginiana) in central tennessee.thirteen species of ectoparasitic (12) or phoretic (1) arthropods were collected from 26 adult virginia opossums, didelphis virginiana, live-trapped from april through september 1987 in davidson county, tennessee. the cat flea ctenocephalides felis and the american dog tick dermacentor variabilis were the predominant species with respect to mean intensity and prevalence. the macronyssid mite ornithonyssus wernecki and the atopomelid mite didelphilichus serrifer, both specific parasites of this h ...19902380870
intrinsic competence of three ixodid ticks (acari) as vectors of the lyme disease spirochete.we compared the intrinsic vector competence of ixodes dammini spielman et al., dermacentor variabilis (say), and amblyomma americanum (l.) for the lyme disease spirochete (borrelia burgdorferi johnson et al.) on prudence island, rhode island, a lyme disease-endemic site where all three ticks occur together. natural and experimental spirochete infection rates were determined in those ticks and their degree of contact with white-footed mice (peromyscus leucopus), the principal reservoir host, was ...19902388239
management of lone star ticks (acari: ixodidae) in recreational areas with acaricide applications, vegetative management, and exclusion of white-tailed deer.a project on management of lone star ticks, amblyomma americanum (l.), at land between the lakes, a tennessee valley authority recreational area in kentucky-tennessee, during 1984-1988, demonstrated the effectiveness and economics of three control technologies. acaricide applications (chlorpyrifos at 0.28 kg [ai]/ha), vegetative management (mowing and removal of 40% overstory and 90-100% of midstory, understory, and leaf litter), and host management (white-tailed deer exclusion from a 71-ha camp ...19901696994
experimental infection of six species of ixodid ticks with dugbe virus (family bunyaviridae, genus nairovirus).the vector potential of each of 6 species of colonized north american and african ixodid ticks was assessed by intracoelomic inoculation with dugbe virus (ibar 1792, 14th passage in suckling mouse brain) and viral titers were monitored after selected incubation periods. persistence of dugbe virus for greater than or equal to 53 days in 5 species (dermacentor andersoni, d. variabilis, amblyomma americanum, rhipicephalus appendiculatus, and r. sanguineus) indicates that infection occurred. viral t ...19892496609
control of lone star ticks (acari: ixodidae) on spanish goats and white-tailed deer with orally administered ivermectin.ivermectin administered orally to spanish goats, capra hircus (l.), or to white-tailed deer, odocoileus virginianus (zimmerman), was highly effective against lone star ticks, amblyomma americanum (l.). for spanish goats, daily oral doses of 20 micrograms/kg resulted in greater than or equal to 2 ppb ivermectin in the blood. this level was sufficient to cause greater than 95% reduction of estimated larvae from feeding ticks. a bioassay with horn flies, haematobia irritans (l.), was developed to e ...19892607030
sites of attachment and density assessment in amblyomma americanum (acari: ixodidae) on nursing beef calves.primary attachment sites of male, female, and nymphal amblyomma americanum (l.) on nursing beef calves were the axillary, inguinal and perianal areas of the body. about 50% of all larvae fed on the ears. a density assessment method which uses the number of a. americanum on less than or equal to 5 body areas to predict the total number of ticks (tick burden) on the left side of a calf is presented. estimates of tick burden for male, female, nymphal and larval a. americanum each require examinatio ...19892714123
habitat use by cattle affects host contact with lone star ticks (acari: ixodidae).the location of zebu, british, and zebu x british cattle in tick-infested plots from march through september 1986 was compared with the location of amblyomma americanum l. in the same plots. in april, may, and june, zebu cattle spent less time (9.3-19.4%) in tick-infested quadrats than british (44.4-63.9%) or zebu-british (49.0-69.3%) cattle. in the same months, the probability that a. americanum would contact zebu cattle (1.5-19.6%) was significantly lower than for british (34.7-41.7%) or zebu- ...19892754101
characterization of ixodid tick salivary-gland gene products, using recombinant dna technology.ticks secrete an array of lesion-maintenance factors into the host via the salivary glands while feeding, some of which elicit an immune response by the host that adversely affects the ability of the tick to feed and reproduce. our approach to characterizing these factors has been to make expression libraries from mrna of salivary glands (from unfed and 3-day-feeding amblyomma americanum females) which will serve as sources of the genes (clones) that code for them. thus far, we have detected 10 ...19892758938
polyfluoro 1,3-diketones as systemic insecticides.a series of aryl polyfluoro 1,3-diketones were examined for systemic ectoparasiticidal activity in cattle. the compounds demonstrated efficacy against several economically important species of insects and acarina. at dosages of 5 mg/kg x1 or 0.35 mg/kg per day intraruminally, activity was observed against blowfly larvae (phormia regina), adult stable fly (stomoxys calcitrans), and lone star tick (amblyomma americanum). in vivo activity was not directly related to in vitro activity, showing a str ...19892769686
female mediation of feeding time on cattle in adult lone star ticks (acari: ixodidae).the extended feeding time response on cattle of adult lone star ticks, amblyomma americanum (l.), during november through december is facultative and is mediated by the female. mean feeding time (11.8 d) in female ticks taken from the field and held at 14:10 (l:d) photoperiod and 25 degrees c for greater than or equal to 15 d was significantly shorter (p = 0.05) than for females taken directly from the field and fed on cattle (14.6 d). mean feeding times of females from the field paired to males ...19892769719
effect of age on cholinesterase activity and protein of unfed larval ticks.the cholinesterase (ache) activity and total protein in homogenates of unfed larvae of amblyomma americanum (l.), a. cajennense (fabricius), a. maculatum koch, anocentor nitens neumann, and boophilus microplus (canestrini) were determined weekly in ticks 1-6 wk of age. there was a considerable variation in total protein, ache, and the ratio of ache to protein as the ticks aged. however, ache activities were constant or increased with age and total protein levels were constant or decreased. no di ...19892918444
comparison of flagging, walking, trapping, and collecting from hosts as sampling methods for northern deer ticks, ixodes dammini, and lone-star ticks, amblyomma americanum (acari:ixodidae).ticks were sampled by flagging, collecting from the investigator's clothing (walking samples), trapping with dry-ice bait, and collecting from mammal hosts on fire island, ny, u.s.a. the habitat distribution of adult deer ticks, ixodes dammini, was the same in simultaneous collections from the investigator's clothing and from muslin flags. walking and flagging samples can both be biased by differences between investigators, so the same person should do comparative samples whenever possible. walk ...19892806016
reduction of the amblyomma americanum (acari: ixodidae) population at fort a.p. hill, virginia, by aerial application of diazinon granules: correlation of percentage control with received dose.habitat (280 ha) of lone star tick, amblyomma americanum (l.), was aerially treated with 14% diazinon granules. concentration of acaricide reaching the forest floor ranged from 0.02 to 1.25 kg (ai)/ha. greater than 85% control was achieved at study plots receiving the highest dose. a positive correlation was shown between dose received at the forest floor and percentage of control of adults (r = 0.91) and nymphs (r = 0.86).19892795622
ability to ixodes scapularis, dermacentor variabilis, and amblyomma americanum (acari: ixodidae) to acquire, maintain, and transmit lyme disease spirochetes (borrelia burgdorferi). 19883193425
susceptibility of amblyomma americanum to natural and experimental infections with theileria cervi.one hundred fifty amblyomma americanum were examined between march and september 1986 from cookson hills wildlife refuge in eastern oklahoma (usa). of these ticks, 11% (17 of 150) were infected with theileria cervi. field-collected nymphal ticks had an 8% (3 of 37) prevalence of infection averaging 1.0 infected acini/nymph. female ticks had a 16% prevalence of infection averaging 1.6 infected acini/female; t. cervi was not observed in salivary glands of field-collected male ticks. when laborator ...19883193564
characterization of tick antigens inducing host immune resistance. ii. description of rabbit-acquired immunity to amblyomma americanum ticks and identification of potential tick antigens by western blot analysis.feeding by adult amblyomma americanum ticks induced a level of immunity in rabbits to subsequent tick feeding that resulted in a significant decrease in tick feeding success and fecundity. histological analysis of tick feeding sites in hosts expressing resistance revealed a predominant eosinophil response, with weak basophil and neutrophil infiltrates. while the basophil was never the dominant granulocyte at the tick feeding sites in resistant hosts, this cell exhibited the greatest increase in ...19883291384
lone star tick (acari: ixodidae) management by host manipulation through habitat modification. 19883351876
seasonal changes in feeding on cattle and reproduction by amblyomma americanum (acari: ixodidae) under field conditions. 19883357171
evidence for regurgitation by amblyomma americanum.western blot analysis of amblyomma americanum-derived midgut extracts using tick-resistant rabbit and guinea pig antisera demonstrated several unique polypeptides ranging from 7 to 355 kda. these polypeptides were not observed in similar blots of salivary gland extracts or serum from the respective host animals. the data from this study suggest that female a. americanum ticks regurgitate gut material during feeding. such a finding explains the ability of immunization with gut-derived preparation ...19883420794
western blot analysis of amblyomma americanum-derived stage-specific and shared antigens using serum from guinea pigs expressing resistance.serum from guinea pigs expressing resistance to larval, nymphal and adult amblyomma americanum ticks was used in western blot analyses to identify potential antigens from egg, larval and nymphal, and female salivary gland extract preparations. the results demonstrate multiple antigens unique to each life stage, as well as several shared proteins between the three life stages. however, it appears as if two particular proteins of 25 and 38 kda may be more important than others, based upon their pr ...19882455376
medium-sized mammal hosts of amblyomma americanum and dermacentor variabilis (acari: ixodidae) at land between the lakes, tennessee, and effects of integrated tick management on host infestations. 19883204625
effects of photoperiod on nymphal diapause in amblyomma americanum (acari: ixodidae). 19883204627
ticks parasitizing humans in a lyme disease endemic area of southern new york state.a total of 126 tick specimens were submitted by tick bite victims to the westchester county department of health, white plains, new york, and to the new york medical college, medical entomology laboratory, armonk, new york, during 1985. these included 96 (76.2%) ixodes dammini, 26 (20.6%) dermacentor variabilis, and two (1.6%) amblyomma americanum. i. dammini parasitism was reported during all months of the year except december and february and involved all life stages. only d. variabilis adults ...19883189288
abundance, attachment sites, and density estimators of lone star ticks (acari: ixodidae) infesting white-tailed deer. 19883404547
effects of season and area on ectoparasites of white-tailed deer (odocoileus virginianus) in mississippi.nine species of ectoparasites (4 acari, 2 mallophaga, 1 anoplura, 1 diptera, and 1 siphonaptera) were recovered from 126 white-tailed deer collected in northern, central, and southern mississippi. intensity and prevalence of adults of ixodes scapularis and larvae, nymphs, and adults of amblyomma americanum varied significantly over collection periods, but not between host sexes. lipoptena mazamae occurred on deer from only one study area. although individual deer were heavily parasitized by tric ...19873586204
a seed tick infestation of the conjunctiva: amblyomma americanum larva.ticks commonly infest humans but rarely bite the eyes. they are vectors of disease; therefore, rapid and complete removal is important. their attachment can be so secure that removal is difficult or hazardous. we excised a larval form of amblyomma americanum from a woodsman's conjunctiva.19873566026
estimation and economic implications of lone star tick (acari: ixodidae) infestation on weight gain of cattle, bos taurus and bos taurus x bos indicus. 19873584640
effects of gamma radiation on spermatogenesis and fertility of male amblyomma americanum (acari: ixodidae).amblyomma americanum males were treated with 0.5, 1, 2, 3, 4, 8, and 16 krad of gamma radiation. testes of ticks treated with 2, 3, 4, 8, and 16 krad were smaller than those of ticks irradiated at lower levels and controls. no recognizable alteration in timing of spermatogenesis was noted among the different radiation groups, but severe breakdown and depletion of germinal cells was noted at 4, 8, and 16 krad. percent hatch of larvae from crosses of irradiated males and untreated females decrease ...19873585625
computer simulation of population dynamics of the lone star tick, amblyomma americanum (acari: ixodidae). 19873585931
role of small mammals in population dynamics and dissemination of amblyomma americanum and dermacentor variabilis (acari: ixodidae) at land between the lakes, tennessee. 19873295243
computer simulation of area-wide management strategies for the lone star tick, amblyomma americanum (acari: ixodidae). 19873669024
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