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venezuelan equine encephalomyelitis virus infection in and transmission by the tick amblyomma cajennense (arachnida: ixodidae).to assess a possible role of ticks as the maintenance host for epizootic strains of venezuelan equine encephalomyelitis (vee) virus, laboratory experiments were conducted to determine if ticks could become infected, maintain, and transmit the virus. larval and nymphal amblyomma cajennense (f.) and larval dermacentor nitens neumann ticks were exposed to epizootic vee virus (trinidad donkey strain) by allowing them to feed on viremic guinea pigs (strain 13). in a. cajennense, transstadial transmis ...19911875367
dissemination, replication, and trans-stadial persistence of dugbe virus (nairovirus, bunyaviridae) in the tick vector amblyomma variegatum.the dissemination and replication of dugbe (dug) virus and its tissue tropisms in the tick vector amblyomma variegatum were examined by immunohistochemical analysis using specific antibody, in situ hybridization with a viral-complementary riboprobe, and infectivity assays of dissected tissues. dugbe virus was localized in both unfed and feeding adults inoculated as nymphs or orally infected by capillary feeding, and in nymphs infected by capillary feeding. in non-feeding ticks, the main sites of ...19911867347
role of genital sex pheromones in amblyomma americanum and a. maculatum (acari: ixodidae).amblyomma americanum and a. maculatum were studied to determine if genital sex pheromones were present, as reported in dermacentor species. chemical analysis of methanol extracts of the anterior reproductive tracts (source of the genital sex pheromone) of a. americanum and a. maculatum confirmed the presence of the same fatty acids that act as components of the genital sex pheromone in dermacentor variabilis and d. andersoni. in both amblyomma species, removal of the anterior reproductive-tract ...19911860418
histopathology of attachment sites of amblyomma variegatum and rhipicephalus appendiculatus on zebu cattle of varying resistance to ticks.mature, unimproved east african zebu cattle (bos indicus) naturally exposed to ticks from birth were ranked for resistance to ticks by repeated whole-body counts of infesting ticks. four cattle of high resistance, four of low resistance and two of intermediate resistance were artificially infested with nymphs of amblyomma variegatum and rhipicephalus appendiculatus. two friesian cattle (bos taurus) with less exposure to ticks were similarly infested. biopsies of tick attachment sites were examin ...19911858290
predation on livestock ticks by chickens.chickens were shown to be natural predators of ticks. rhipicephalus appendiculatus were recovered in large numbers from the crops and gizzards of chickens which had scavenged for 30 min-1 h among tick-infested cattle. other ticks recovered were amblyomma variegatum and boophilus decoloratus. the numbers of ticks recovered ranged from 3 to 331, with an average of 81 per chicken. cattle facilitated the predation of ticks by certain behavioural actions. chickens also picked up both engorged and une ...19911858289
[sites of fixation of amblyomma variegatum ticks on their hosts in guadeloupe (french west indies)].the examination of 458 domestic and wild hosts of the tick a. variegatum allowed to collect 8,581 larvae, 646 nymphs, 685 adults in 9 different animal species and to determine their preferential attachment sites. in another survey on 15 goats and 5 cattle, 12,345 larvae, 2,111 nymphs and 4,625 adults were counted on the anatomic sites. immature ticks feed preferentially on the head, neck and limbs of birds, the limbs of mongoose and the limbs and ears of dogs, goats and cattle. adults feed only ...19911843828
[seasonal dynamics of tick infestations in cattle (ixodoidea) in cattle sold in the area of yaoundé, cameroon].among the tick species identified in cameroon, eight were found in cattle in yaoundé: amblyomma variegatum, boophilus annulatus, b. decoloratus, haemaphysalis aciculifer, hyalomma nitidum, rhipicephalus longus, rh. lunulatus, rh. sulcatus. three species, hm. aciculifer, h. nitidum and rh. lunulatus, were identified for the first time in yaoundé. rhipicephalus and hyalomma were abundant during the rainy season. boophilus was abundant throughout the year, with a maximum concentration at the end of ...19911824137
ticks (ixodidae) parasitizing humans in four provinces of north-western argentina.in four provinces of north-western argentina (catamarca, jujuy, salta and tucumán), between march 1976 and march 1990, 514 ticks were found on humans. they were identified as rhipicephalus sanguineus group (1 male), boophilus microplus (6 male, 1 female), amblyomma parvum (9 male, 13 female), a. cajannense (35 male, 30 female, 81 nymphs), a. neumanni (33 male, 41 female, 144 nymphs) and amblyomma spp. (10 nymphs, 110 larvae). most of the ticks were from the phytogeographical region of chaco, one ...19911809248
crimean-congo haemorrhagic fever virus replication in adult hyalomma truncatum and amblyomma variegatum ticks.the kinetics of the replication of the crimean-congo haemorrhagic fever virus (cchfv) was studied in intra-anally inoculated adult hyalomma truncatum and amblyomma variegatum ticks. the virus was re-isolated by suckling mouse inoculation and revealed by antigen capture with ground ticks and indirect immunofluorescence of haemolymph. the virus was detected in ticks in the first hours post-inoculation (p.i.) and its replication was observed from 36 h p.i. onwards. virus titre reached a maximum wit ...19911803413
re-assessment of tick control after immunization against east coast fever in the eastern province of zambia.east coast fever, caused by the protozoon theileria parva and transmitted by the ixodid tick rhipicephalus appendiculatus is one of the most important cattle diseases in east and central africa, responsible for considerable direct losses and necessitating expensive control measures. traditionally, the disease was controlled by means of intensive tick control. the belgian animal disease control project was requested to study the disease epizootiology and vector ecology in order to formulate and i ...19911793284
the occurrence of streptothricosis and its association with amblyomma variegatum ticks in st. lucia.cattle, horses, sheep and goats in all areas of st. lucia were examined to ascertain the prevalence and distribution of streptothricosis on the island and to investigate its association with the occurrence of amblyomma variegatum ticks. although the disease was found to occur throughout st. lucia it was more prevalent and generally of a more severe form in areas where a. variegatum ticks are present. a tick control programme in the north of the island appeared to have resulted in a dramatic redu ...19911789020
parasite burdens and host susceptibility of zebu and n'dama cattle in village herds in gambia.helminth and tick burdens of n'dama cattle kept in village herds were compared with parasite burdens in zebu cattle introduced into the same herds. the animals were monitored regularly for tick and helminth burdens, and blood samples were examined for differential white cell counts. the zebu were found to have significantly higher numbers of endo- and ectoparasites. the n'dama had significantly higher levels of circulating eosinophils than the zebu during peak parasite burdens. no difference in ...19911788936
a comparison of the reproductive parameters of females of two reptile tick species.in comparisons of females of two reptile tick species aponomma hydrosauri and amblyomma limbatum, ap. hydrosauri was initially larger, and after mating on the host engorged faster and remained attached for a shorter time before completing engorgement and detaching. amb. limbatum had a longer period of engorgement, and achieved a greater engorged weight. engorged amb. limbatum females laid significantly more eggs than equivalent sized ap. hydrosauri. although the two species are ecologically simi ...19911787031
seasonal occurrence of the bont tick (amblyomma hebraeum) in the southern lowveld of zimbabwe.the seasonal occurrence of the bont tick amblyomma hebraeum was recorded over 2 years on 20 brahman cattle held in a 240-ha paddock at mbizi in southeastern zimbabwe. the cattle were infested with all life cycle stages throughout the study period, and no clearly defined seasonal patterns were recorded. males remained attached for up to about 6 months, and consequently accumulated on the cattle and outnumbered females considerably. the cattle did not acquire resistance to a. hebraeum and the abun ...19911786747
comparison of known and suspected pheromonal constituents in males of african ticks, amblyomma hebraeum koch and amblyomma variegatum (fabricius).three low molecular weight compounds were found in hexane:diethyl ether extracts of fed males of the african ticks, amblyomma variegatum (tropical bont tick) and a. hebraeum (bont tick), namely, o-nitrophenol, methyl salicylate and 2,6-dichlorophenol. these same compounds were also fond in a rinse of fed a. variegatum males, but were absent or present in only trace amounts in a rinse of fed a. hebraeum males, o-nitrophenol and methyl salicylate were present in much higher concentrations (i.e., a ...19911786745
the life cycle of amblyomma parvum aragao, 1908 (acari: ixodidae) under laboratory conditions.a colony of amblyomma parvum was started with engorged females collected from cattle in the province of salta (25 degrees 01' s, 63 degrees 56' w), argentina. the progeny of those ticks were fed on rabbits and the non-parasitic stages maintained at 27 +/- 1 degrees c, 83-86% rh in darkness. the life cycle (prefeeding period not evaluated) had a mean duration of 99.6 days. the mean time (days) for the different phases of the cycle were as follows: feeding period of females, 8.0; pre-oviposition p ...19911786743
transient infections of goats with a novel spotted fever group rickettsia from zimbabwe.a novel spotted fever group rickettsia has recently been isolated from amblyomma hebraeum ticks in zimbabwe. in a survey of 172 goat sera collected throughout zimbabwe the highest prevalence of antibodies reactive with this rickettsia was in the south of the country, the area where a hebraeum is most commonly found. nine goats were infected using male and female a hebraeum taken from a tick line shown to be infected with the novel rickettsia. by week 3 after infection, seroconversion occurred in ...19911780580
parasites of domestic and wild animals in south africa. xxix. ixodid ticks on hares in the cape province and on hares and red rock rabbits in the orange free state.one hundred and seventeen scrub hares, lepus saxatilis, were examined for ixodid ticks in various regions of the cape province. they were infested with 18 tick species and the seasonal abundances of the immature stages of amblyomma hebraeum, amblyomma marmoreum, hyalomma marginatum rufipes, rhipicephalus appendiculatus and rhipicephalus glabroscutatum and all stages of rhipicephalus oculatus and the adults of a rhipicephalus sp. (near r. oculatus) were determined. seventy-two scrub hares on 3 fa ...19911780126
a cloned dna probe identifies cowdria ruminantium in amblyomma variegatum ticks.heartwater, caused by cowdria ruminantium and transmitted by ticks of the genus amblyomma, is a constraint to ruminant animal production in sub-saharan africa. this rickettsial disease could spread from endemically infected areas of sub-saharan africa and certain caribbean islands to other countries, including the united states, in which amblyomma ticks exist. to detect c. ruminantium in tick vectors and animals, we made dna probes from c. ruminantium dna isolated from endothelial cell cultures. ...19911774264
resistance of indigenous african cattle to the tick amblyomma hebraeum.field collections of amblyomma hebraeum koch adults from six breeds of cattle were conducted on two farms in the northern transvaal. the density of a. hebraeum adults was highest on simmentaler cows, followed by santa gertrudis, africaner, bonsmara, brahman and nguni. indigenous breeds, namely nguni, bonsmara and africaner, have the highest level of resistance to a. hebraeum. the relationship between tick abundance and the high level of resistance found in the indigenous breeds is discussed.19911773680
acaricide susceptibility of amblyomma variegatum (acari:ixodidae) from puerto rico and guadeloupe.base-line data on acaricide susceptibility of larvae and of larvae and nymphs of the tropical bont tick, amblyomma variegatum (fabricius), from puerto rico and guadeloupe were collected on 14 and six compounds, respectively. nymphs 7-8 weeks old from puerto rico and guadeloupe, and larvae 3-4 weeks old from guadeloupe, were exposed for 24 or 48 h to residues of acetone dilutions of technical-grade or commercial formulations of acaricides on disposable glass pipettes. effectiveness of the acarici ...19911773678
predators of the tick amblyomma variegatum (acari: ixodidae) in guadeloupe, french west indies.some vertebrate species in guadeloupe are predators of free or parasitic stages of amblyomma variegatum (fabricius). among birds, 1.9% of the 421 identified animals found in the stomachs of grackles (quiscalus lugubris), 1.6% of the 364 animals found in the stomachs of free-ranging chickens, and 0.3% of the 4642 animals found in the stomach of cattle egrets (bubulcus ibis) were a. variegatum ticks. the most efficient predator of ticks was the tropical fire ant, solenopsis geminata, which was obs ...19911773677
rectal infusion and aspiration of material through the guts of ixodid ticks (acari: ixodidae).a technique for inoculating and removing substances via the anus of vector ticks was devised to define features of vector competence precisely. calibrated inocula (greater than 5 nanoliter) containing aqueous dye and polystyrene beads as well as infectious agents were infused into the rectal sacs of ticks using glass microcapillary pipettes placed within the expanded anal orifice. the guts of preadult and adult ticks, ixodes dammini spielman, clifford, piesman & corwin, dermacentor variabilis (s ...19911770516
tick infestations of white-tailed deer in alabama.four species of ticks were collected from 537 white-tailed deer (odocoileus virginianus), examined during the hunting seasons (november to january) of 1988-89 and 1989-90 at selected locations in alabama (usa). ixodes scapularis was the most common tick recovered (2,060 specimens) and infested 54% of the deer. dermacentor albipictus was the second most frequent tick (1,253 specimens) and infested 15% of the deer. amblyomma americanum was the third most frequent tick (315 specimens) and infested ...19911758026
amblyomma americanum: characterization of salivary prostaglandins e2 and f2 alpha by rp-hplc/bioassay and gas chromatography-mass spectrometry.secretagogue-induced saliva of the tick, amblyomma americanum (l.) was fractionated by reversed-phase-high-performance liquid chromatography (rp-hplc) and bioassayed in smooth muscle preparations. material with retention times of authentic prostaglandin e2 (pge2) and prostaglandin f2 alpha (pgf2 alpha) were found to cause contraction of preparations of rat colon and rat stomach strips. gas chromatography-mass spectra of selected ions of both hplc-purified fractions confirmed the existence of pge ...19921730268
putative new expression of genes in ixodid tick salivary gland development during feeding.poly(a+) mrna-enriched fractions from salivary glands of partially fed amblyomma americanum female ticks were translated in vitro with a rabbit reticulocyte translation system. translated proteins were labeled with [35s]methionine, separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis, and identified by autoradiography. thirty major identifiable polypeptides with molecular weights ranging from 14 to 136 kda were synthesized from mrna isolated from salivary glands of ticks weighi ...19911710268
detection of an arbovirus in an invertebrate and a vertebrate host using the polymerase chain reaction.the ability of the polymerase chain reaction (pcr) to diagnose an arboviral infection in an arthropod vector or a mammalian host was examined. dugbe (dug) viral rna was detected in rna extracts from infected tissue samples by reverse transcription and enzymatic amplification of the resulting cdna using taq dna polymerase, followed by characterisation of the amplified product by agarose gel electrophoresis or dot-blot hybridisation. viral rna was detected in the organs and haemolymph of infected ...19901707893
management of lone star ticks (acari: ixodidae) in recreational areas with acaricide applications, vegetative management, and exclusion of white-tailed deer.a project on management of lone star ticks, amblyomma americanum (l.), at land between the lakes, a tennessee valley authority recreational area in kentucky-tennessee, during 1984-1988, demonstrated the effectiveness and economics of three control technologies. acaricide applications (chlorpyrifos at 0.28 kg [ai]/ha), vegetative management (mowing and removal of 40% overstory and 90-100% of midstory, understory, and leaf litter), and host management (white-tailed deer exclusion from a 71-ha camp ...19901696994
the role of amblyomma variegatum in the transmission of heartwater with special reference to guadeloupe.heartwater has been diagnosed in guadeloupe, marie-galante, and antigua; it induces important losses among goats and european or cross-bred cattle when local zebu creole are highly resistant to infection. amblyomma variegatum is the vector of the disease in the caribbean. the tick strain of martinique, occurring in that island that has apparently been disease-free since 1948, has not lost its ability to experimentally transmit the disease. in guadeloupe 97% of nymphs and nearly 100% of adults fe ...19921626882
inability of ixodes cookei and amblyomma americanum nymphs (acari: ixodidae) to transmit borrelia burgdorferi.the vector competency of ixodes cookei packard and amblyomma americanum (l.) for borrelia burgdorferi was studied using syrian hamsters. ixodes dammini spielman, clifford, piesman & corwin were used as controls. darkfield and immunofluorescent examinations of midgut diverticula revealed b. burgdorferi spirochetes in 32 of 36 (88.9%) i. dammini larvae, 5 of 36 (13.9%) i. cookei larvae, and 7 of 36 (19.4%) a. americanum larvae within 48 h after feeding on infected syrian hamsters. b. burgdorferi w ...19921625302
quantitative evaluation of sampling methods for ixodes dammini and amblyomma americanum (acari: ixodidae).three tick-sampling methods (dry ice-baited tick traps, cloth drags, and ambulatory human host) were evaluated to determine which technique yielded the greatest capture of host-seeking stages of ixodes dammini spielman, clifford, piesman & corwin and amblyomma americanum (l.). the most reliable method, catching more stages and significantly more numbers of i. dammini and a. americanum; was dry ice-baited tick traps. there were no significant differences between the drag and human-host methods fo ...19921625293
laboratory and field observations on anti-tick properties of the plant gynandropsis gynandra (l.) brig.a shrubby plant, abundant in east kenya, gynandropsis gynandra (l.) brig., was shown to exhibit repellent and acaricidal properties to larvae, nymphs and adult rhipicephalus appendiculatus and amblyomma variegatum ticks. all stages of ticks avoided the leaves of the plant and a high percentage of the ticks which were continuously exposed to its leaves died; surviving ticks were weak and inactive but regained activity when exposed to fresh air. the effectiveness of the plant's leaves as a repelle ...19921615623
interspecific differences in the movements of female ticks on reptiles.movements of females of two reptile ticks, amblyomma limbatum and aponomma hydrosauri were studied to determine whether the behaviour of the species on hosts was similar or different. experiments were conducted to determine if the movement of females on hosts prior to mating was influenced by the delayed attachment of conspecific males. a field experiment was also undertaken to determine whether data obtained during laboratory experiments reflected the behaviour of females on hosts in the field. ...19921587690
amblyomma americanum (l.): protein kinase c-independent fluid secretion by isolated salivary glands.protein kinase c activity was partially purified from tick salivary glands by fast protein liquid chromatography anion-exchange chromatography. enzyme activity was stimulated by ca2+, phosphatidylserine, and diacylglycerol with the highest activity observed in the presence of all three modulators. enzyme activity was inhibited by a synthetic pseudosubstrate peptide with an amino acid sequence resembling the protein kinase c substrate phosphorylation site. the protein kinase c activator, 1-oleoyl ...19921582485
a comparison of the attraction of nymphs and adults of the ticks amblyomma hebraeum and a. variegatum to carbon dioxide and the male-produced aggregation-attachment pheromone.the attraction of nymphs of the ticks amblyomma hebraeum and a. variegatum to co2 alone and co2 together with the male-produced aggregation-attachment pheromone (aap) was investigated. matching experiments on the attraction of the adults of the two species were run for comparison. nymphs of a. hebraeum were strongly attracted to sources of co2 and co2/aap at distances of 10 and 15 m. at distances of 20 and 25 m significantly more nymphs were attracted to co2/aap than to co2 alone. adults of a. h ...19921563302
distribution, density, and lyme disease spirochete infection in ixodes dammini (acari: ixodidae) on white-tailed deer in maryland.a statewide survey of ticks parasitizing white-tailed deer was carried out in maryland during november 1989 to assess the status of the deer tick, ixodes dammini spielman, clifford, piesman & corwin, the major vector of lyme disease in the northeastern united states. ticks were collected from deer carcasses brought in by hunters at 23 check stations (one per county). a total of 3,437 i. dammini were collected from 538 of 1,281 deer (42%), together with 2,013 dermacentor albipictus (packard) and ...19921552529
oral secretion elicited by effectors of signal transduction pathways in the salivary glands of amblyomma americanum (acari: ixodidae).the abilities of various effectors of signal pathways in tick salivary glands to stimulate oral secretions in partially fed amblyomma americanum (l.) female ticks were compared. pilocarpine stimulated secretion the most rapidly following tick stimulation, but its effectiveness declined with time and subsequent injections. secretion rates induced by dopamine and theophylline increased with time of collection and additional injections and were as effective as pilocarpine after 60 min. activators o ...19921552527
a case of gynandromorphism in amblyomma hebraeum (acari: ixodidae).a gynandromorph specimen of amblyomma hebraeum was found on a goat on which adult ticks were bred in the laboratory.19921552517
olfactory responses of adult amblyomma hebraeum and a. variegatum (acari: ixodidae) to attractant chemicals in laboratory tests.unfed adults of the african ticks, amblyomma hebraeum koch and a. variegatum (fabricius), important vectors of human and animal diseases, were exposed to volatile compounds in an olfactometer in efforts to identify both tick-produced or synthetic chemicals capable of eliciting an attraction response. a formula, relative efficacy of attraction, was devised for comparison of responses between species and sexes to a particular test stimulus, or within a homogeneous population to different stimuli. ...19921526203
the use of time-series analysis to forecast bont tick (amblyomma hebraeum) infestations in zimbabwe.studying the dynamics of tick infestations on cattle is an essential step in developing optimal strategies for tick control. successful strategic tick control requires accurate predictions of when tick infestations will reach predetermined threshold levels. in the case of amblyomma hebraeum, earlier work has shown that there is no consistent pattern of seasonal activity. this means that a statistical model for predicting a. hebraeum infestations cannot reliably use climatic factors as the only i ...19921526202
effects of the avermectin analogue mk-243 on vitellogenesis and reproduction in the ixodid tick, amblyomma hebraeum.a dose-dependent reduction in weight of total egg mass and a slightly increased oviposition latency were observed following injection of the avermectin analogue mk-243 (4"-epi-methylamino-4"-deoxyavermectin b1) directly into the haemocoel of engorged female amblyomma hebraeum koch (acari: ixodidae). egg laying was almost completely inhibited at 100 micrograms/kg body weight. mk-243 markedly inhibited ovary development and vitellogenesis. ticks treated with 100 micrograms mk-243/kg also had one-t ...19921526201
description of aegyptianella botuliformis n. sp. (rickettsiales: anaplasmataceae) from the helmeted guineafowl, numida meleagris.aegyptianella botuliformis n. sp. (rickettsiales: anaplasmataceae) isolated from helmeted guineafowls numida meleagris from the kruger national park is described. the rickettsia occurs within a membrane-bound vacuole in the cytoplasm of erythrocytes with up to 8 organisms in a mature inclusion. the initial body resembles that of aegyptianella pullorum. the tightly packed, sausage-shaped intermediate forms are a distinctive morphological feature, seen as irregular, pleomorphic forms under light m ...19921513599
adult amblyomma hebraeum burdens and heartwater endemic stability in cattle.several conclusions of importance to studies on the epidemiology of heartwater were drawn from an investigation in which the numbers of adult amblyomma hebraeum ticks, to which a closed herd of hereford cattle were exposed over a period of 6 1/2 years, were manipulated. with a tick cowdria ruminantium infection rate of 3-5%, an endemically stable situation was created by dipping the herd only when an average of 10 adult male and female a. hebraeum ticks were counted on 10 animals. when the avera ...19921513597
ectoparasites of the blackbuck antelope (antilope cervicapra).fifty-two free-ranging blackbuck antelope (antilope cervicapra) from texas were examined for ectoparasites. two species of sucking lice (anoplura), one species of chewing louse (mallophaga), one species of louse fly (diptera), and three species of ticks (acari) were found. this is the first report of the anoplurans linognathus cervicaprae and l. pithodes from the western hemisphere. the southern deer ked (lipoptena mazamae), the winter tick (dermacentor albipictus), and the rabbit tick (haemaphy ...19921512888
monthly incidence of theileria cervi and seroconversion to babesia odocoilei in white-tailed deer (odocoileus virginianus) in texas.monthly monitoring of fawns collected from an area in texas endemic for theileria cervi and babesia odocoilei showed that transmission of t. cervi occurred during july and august, a time period consistent with the occurrence of amblyomma americanum. seroconversion to b. odocoilei occurred during october to december and possibly continued through january and february. the time of seroconversion was more suggestive of transmission of b. odocoilei by ixodes scapularis than by amblyomma americanum.19921512881
borrelia sp. in ticks recovered from white-tailed deer in alabama.six hundred sixty-five hunter-killed white-tailed deer (odocoileus virginianus) from 18 counties in alabama (usa) were examined for ticks. most of the collections were made at state-operated wildlife management areas. four species of ticks (n = 4,527) were recovered: the lone star tick amblyomma americanum (n = 482); the gulf coast tick a. maculatum (n = 11); the winter tick dermacentor albipictus (n = 1,242); and the black-legged tick ixodes scapularis (n = 2,792). fifty-six percent of the tick ...19921512879
a contribution to the anatomy and histology of the female reproductive system of amblyomma cajennense (acarina: ixodidae).the genital system of the tick amblyomma cajennense is composed of several parts: a single tubular ovary where a great number of ova develop except for a longitudinal groove on its dorsal surface, with feeding the ova develop multiplying the size and weight of the ovary, as they reach maturity they are ovulated into the lumen. two oviducts are connected to the ovary on each side, these thicken at a certain region forming the knee shaped ampulla. this significant region is where great numbers of ...19921500779
a freeze etching study on the distribution of nuclear pores during spermatogenesis in ticks.in this study the freeze-fracturing and freeze-etching techniques was used to study pore distribution during spermatogenesis in ticks. three species of ixodid ticks were studied: hyalomma dromedarii, h. marginatum and amblyomma hebraeum. in all three species of the ticks examined, the nuclei of the early stages of male germ cells showed varying degrees of aggregation into pore-rich and pore-poor areas. spermatocytes demonstrated significant pore clustering in pore-rich areas, leaving areas almos ...19921500776
ultrastructure of symbiont-like microorganisms associated with the sperm of ixodid ticks.the endospermatophores found in the seminal receptacles of three species of ixodid ticks: hyalomma marginatum, h. dromedarii and amblyomma hebraeum were examined. the seminal receptacles showed the presence of symbiont-like microorganisms in association with the sperm cells. these yeast-like microorganisms represent extracellular symbionts, produced by the male and introduced in the genital system of the female through copulation. the symbionts frequently occur freely in large agglomerations sur ...19921500765
attempted transmission of borrelia burgdorferi (spirochaetales: spirochaetaceae) (jdi strain) by ixodes scapularis (acari: ixodidae), dermacentor variabilis, and amblyomma americanum.laboratory-reared ixodes scapularis say, amblyomma americanum (l.), and dermacentor variabilis (say) were fed on new zealand white rabbits experimentally infected with borrelia burgdorferi (jdi strain). at repletion, spirochetes could be detected by dark-field microscopy only in i. scapularis. acquisition rates were 18 and 21%. when previously exposed nymphs of each species were fed on susceptible rabbits, i. scapularis was the only tick of the three species that transmitted b. burgdorferi. when ...19921495078
field evaluation of two formulations of cyfluthrin for control of ixodes dammini and amblyomma americanum (acari: ixodidae).liquid or granular cyfluthrin was applied in the spring and fall to different test plots at a rate of 0.41 (ai) kg/ha to control ixodes dammini spielman, clifford, piesman & corwin and amblyomma americanum (l.). spring application of liquid cyfluthrin showed the most significant decreases of both species. i. dammini nymphs were reduced 97% at 10 d, 100% at 2 mo, and 100% at 1 yr; and a. americanum adults and nymphs were reduced 91-93% at 10 d and 100% at 2 mo posttreatment. granular cyfluthrin a ...19921495073
tick sweep: modification of the tick drag-flag method for sampling nymphs of the deer tick (acari: ixodidae).we describe a version of the standard tick drag-flag modified for use in close-growing and tangled vegetation, as well as under ornamental shrubbery and fallen branches. two major features of the sweep are: (1) it allows the user to remain upright with the flag parallel to the ground, thus sampling effectively beneath low and fallen branches and around shrubs, as well as capturing host-seeking ticks in advance of the operator; and (2) the use of a flannel rubberized-laminate fabric (crib sheet) ...19921495058
feeding efficiency of larval hyalomma truncatum (acari: ixodidae) on hosts previously exposed to ticks.the effect of guinea pig response to feeding larval hyalomma truncatum koch ticks was studied by measuring the percentage of ticks engorging and molting, their engorged weight, and hemoglobin content. four guinea pigs were infested with 100, 200, 400, and 1,000 larvae, respectively, on three occasions at 21-d intervals, followed by a fourth infestation with 500 larvae. during the second, third, and fourth infestations, significantly fewer ticks engorged on each guinea pig than during the first i ...19921495048
tick (acari: ixodidae) attachment cement and salivary gland cells contain similar immunoreactive polypeptides.a specific antiserum (12c) raised to a 90-kda immunogenic component of salivary glands of the tick rhipicephalus appendiculatus recognized similar 90-kda polypeptides from salivary glands of the american dog tick, dermacentor variabilis, and the lone star tick, amblyomma americanum, as well as 70-kda polypeptides in the cement of d. variabilis, a. americanum, and r. sanguineus (brown dog tick). the reduction in size of the polypeptide for these ticks suggests that it is modified in some way duri ...19921495047
parasites of cottontail rabbits of southern illinois.fifteen species of parasites including haemaphysalis leporispalustris, ixodes dentatus, amblyomma americanum, cediopsylla simplex, odontopsyllus multispinosus, cuterebra sp., obeliscoides cuniculi, trichostrongylus calcaratus, trichostrongylus affinis, longistriata noviberiae, dermatoxys veligera, trichuris sp., mosgovoyia sp., taenia pisiformis, and hasstilesia tricolor as well as coccidia oocysts were collected from 96 cottontail rabbits (sylvilagus floridanus) confined to a pen in southern il ...19921491303
synganglial morphology and neurosecretory centers of adult amblyomma americanum (l.) (acari: ixodidae).lone star ticks, amblyomma americanum (l.), were processed by standard histological means for paraffin embedding, sectioning, and staining by the paraldehyde-fuchsin technique. the synganglion is highly condensed around the esophagus and possesses paired optic, cheliceral, palpal, pedal i-iv nerves, and opisthosomal nerves and a single unpaired esophageal nerve. although optic nerves were observed leading from the eyes to the protocerebrum, distinct optic ganglia were not seen in any of the prep ...19921460618
the identification of a shared immunogen present in the salivary glands and gut of ixodid and argasid ticks.the identification of a 70-kda immunogen present in salivary gland extracts of several ixodid species, namely hyalomma truncatum (sweating-sickness-inducing (ss+) and non-inducing (ss-) strains), hyalomma marginatum rufipes and rhipicephalus evertsi evertsi, is reported. the immunogen was identified by western blots using a monoclonal antibody of the igm isotype directed against a 70-kda immunogen present in the salivary glands of (ss-) female h. truncatum ticks. cross-reactivity with the gut of ...19921446571
comparison of the attachment rates of males of the ticks amblyomma hebraeum and a. variegatum to cattle, sheep and rabbits in the absence of aggregation-attachment pheromone.losses in domestic ruminants caused by heartwater (cowdria ruminantium infection) in zimbabwe and mozambique are greater when the vector is amblyomma hebraeum than when the vector is a. variegatum. it has been suggested that the epidemiology of the disease may be influenced by the rates at which unfed adults of these two tick species attach to uninfested hosts (i.e. in the absence of the male-produced aggregation-attachment pheromone [aap]). in this study we confined unfed males of a. hebraeum a ...19921446569
an indirect mechanism by which a protein from the male gonad hastens salivary gland degeneration in the female ixodid tick, amblyomma hebraeum.in the adult female tick, amblyomma hebraeum koch (acari: ixodidae), salivary gland degeneration is triggered by an ecdysteroid, provided the female is above a critical weight (approximately 300-400 mg). in mated females, salivary gland degeneration is virtually complete within 4 days of detachment from the host. in virgin females, salivary gland degeneration is delayed by 4 days. this delay can be reversed by the injection of a male reproductive tract homogenate directly into the hemocoel. in t ...19921446071
perception of breath components by the tropical bont tick, amblyomma variegatum fabricius (ixodidae). ii. sulfide-receptors.wall-pore sensilla in the capsule of haller's organ on foreleg tarsi of the tick, amblyomma variegatum, show multicellular responses upon stimulation with human and bovine breath. filtering breath through charcoal removes the stimulant for some of these receptors. analysis by gas chromatography coupled with olfactory sensillum electrophysiological recordings indicates that an ethanol extract of the breath components trapped on charcoal contains a major stimulant eluting at the same retention tim ...19921432848
acquired immunity to larvae of amblyomma marmoreum and a.hebraeum by tortoises, guinea-pigs and guinea-fowl.a study of acquired resistance in guinea-pigs, guinea-fowl and tortoises to larvae of the ticks amblyomma hebraeum koch and a.marmoreum koch (acari: ixodidae) showed that repeated infestations of the laboratory host resulted in a 29.3-49.4% reduction respectively in weight of engorged larvae. no resistance was induced in guinea-fowl, the natural host for larvae of both species. a small decline in the mean weight of engorged larvae was observed for both species of ticks after three successive inf ...19921421505
influence of exposure period and management methods on the effectiveness of chickens as predators of ticks infesting cattle.experiments undertaken on rusinga island, kenya, indicated that rhipicephalus appendiculatus (neumann) was the predominant tick consumed on tick-infested cattle by chickens, followed by amblyomma variegatum (fabricius), boophilus decoloratus (koch) and rhipicephalus evertsi evertsi (neumann). the number of ticks consumed by chickens released with cattle in a 3 h release period was not significantly different from ticks consumed in a 4 h release period. in a free management system, chickens 1-3 m ...19921413461
comparative infections of epizootic and enzootic strains of venezuelan equine encephalomyelitis virus in amblyomma cajennense (acari: ixodidae).to compare the potential for an enzootic or an epizootic strain of venezuelan equine encephalomyelitis (vee) virus to infect amblyomma cajennense (f.), larval ticks were fed on guinea pigs (strain 13) inoculated with an enzootic viral strain of variant i-e (68u201) or on guinea pigs inoculated with an epizootic strain of variant i-a (trinidad donkey). peak viremias were 10(5.2) plaque-forming units (pfu)/ml and 10(7.3) pfu/ml in guinea pigs infected with enzootic and epizootic viral strains, res ...19921404262
immunization of rabbits against nymphs of amblyomma hebraeum and a. marmoreum (acari: ixodidae).himalayan rabbits immunized with homogenates prepared from nymphs of amblyomma hebraeum koch and a. marmoreum koch ticks developed humoral and probably also cell-mediated immunity to their respective homogenates. beta and gamma globulin levels and numbers of eosinophils and neutrophils increased significantly in inoculated rabbits. the recipient animals developed resistance to homospecific nymphal infestations. cross resistance between the two species was not evaluated. nymphs of both species th ...19921404253
laboratory studies on the life cycle of amblyomma marmoreum (acari: ixodidae) on two different hosts.a comparative study was made of the life cycle of the tortoise tick, amblyomma marmoreum koch, on tortoises and guinea pigs under laboratory conditions. at 25 degrees c and 85% rh with natural day length, duration of off-host stages (preoviposition, oviposition, incubation, and premolt) was similar for ticks fed on both hosts. delay in preoviposition (up to 90 d) was observed in some gravid females. larvae, nymphs, and adults had longer feeding periods on tortoises than on guinea pigs. adult fem ...19921404252
experimental transmission of cowdria ruminantium (rickettsiales) by the american reptile tick amblyomma dissimile koch, 1844.a senegalese isolate of the rickettsia cowdria ruminantium was transmitted transstadially by nymphs of the american reptile tick amblyomma dissimile. only eight nymphs, fed as larvae on a saanen goat reacting to heartwater, were required to transmit fatal heartwater to another susceptible goat. since a. dissimile usually feeds on snakes, iguanas and lizards in central america, the tick is not considered to play a significant role in the transmission of heartwater between ruminants. however, the ...19921395974
comparative study of tick burdens in gudali and wakwa cattle under natural infestation in the subhumid highlands of wakwa, cameroon. preliminary observations.the relative resistance to different cattle ticks of gudali and wakwa cattle with different levels of brahman breeding, grazed on natural pastures in the subhumid tropics of wakwa, cameroon, was assessed using pasture tick infestations. the basic design consisted of 5 young bulls of each breed from different sire herds. tick populations were observed in gudali and wakwa bulls over a period of four weeks when the climate was thought to be highly favourable for the free-living stages of ticks. cou ...19921339999
perception of breath components by the tropical bont tick, amblyomma variegatum fabricius (ixodidae). i. co2-excited and co2-inhibited receptors.wall-pore olfactory sensilla located in the capsule of haller's organ on the tarsus of amblyomma variegatum ticks bear cells responding to vertebrate breath: one of these sensilla contains a co2-excited receptor and a second sensillum has a co2-inhibited receptor. each of these antagonistic co2-receptors, which display typical phasic-tonic responses, monitors a different co2-concentration range. the co2-inhibited receptor is very sensitive to small concentration changes between 0 and ca. 0.2%, b ...19921331433
saliva-activated transmission (sat) of thogoto virus: dynamics of sat factor activity in the salivary glands of rhipicephalus appendiculatus, amblyomma variegatum, and boophilus microplus ticks.thogoto (tho) virus is transmitted from infected to uninfected ticks when co-feeding on uninfected guinea-pigs, even though the guinea-pigs do not develop a detectable viraemia. this form of non-viraemic transmission is potentiated by a factor(s) secreted by the saliva of ticks and hence has been termed saliva-activated transmission (sat). the synthesis of the sat factor by the salivary glands of three ixodid tick species was determined by placing uninfected nymphal ticks on guinea-pigs that wer ...19921326431
[asymptomatic carrier state in creole goats and cattle after recovery from cowdria infection in guadeloupe].creole goats and cattle in guadeloupe can be carriers of cowdriosis (heartwater: cowdria ruminantium) after recovery for a period as long as 11 months in goats and 2 months in cattle. the carrier status was demonstrated by feeding amblyomma variegatum nymphs on recovered animals and the resulting adult ticks on susceptible goats. cowdria ruminantium was not detected permanently during the carrier status.19921301623
[attempt to attract amblyomma variegatum (acarina:ixodina) on cattle previously treated with aggregation-fixation pheromones and a pyrethrinoid acaricide].the application of synthetic pheromonal aggregation-attachment compounds or male tick extracts on certain sites of the body of bull calves reared on infested pastures did not induce any attraction of free adults on these sites. previously attached males were more attractive than synthetic pheromones or natural extracts. very small amounts of flumethrin poured directly on testicles or on the dewalp induced a large decrease in the infestation of these sites.19921298024
parasites of domestic and wild animals in south africa. xxx. ectoparasites of kudus in the eastern transvaal lowveld and the eastern cape province.sets of four kudus were shot and examined for arthropod parasites at approximately monthly intervals from april 1981 to march 1983 in the southern part of the kruger national park, eastern transvaal lowveld. these animals harboured 10 ixodid tick species of which boophilus decoloratus followed by amblyomma hebraeum were the most abundant. the seasonal abundances of these ticks and of amblyomma marmoreum, rhipicephalus appendiculatus, rhipicephalus evertsi evertsi and rhipicephalus zambeziensis w ...19921297956
responses of the ticks amblyomma hebraeum and a. variegatum to known or potential components of the aggregation-attachment pheromone. iv. attachment stimulation of nymphs.ten known or potential components of the aggregation-attachment pheromone (aap) of the ticks amblyomma hebraeum and a. variegatum, as well as a mixture of these components and extracts of prefed males of the two species, were tested as attachment stimulants for nymphs. unfed nymphs were confined in linen bags on the ears of rabbits that had been treated with the test compounds, mixture or extracts; the numbers attached were recorded after 24 h. in a. hebraeum, attachment was induced by four comp ...19921286610
responses of the ticks amblyomma hebraeum and a. variegatum to known or potential components of the aggregation-attachment pheromone. iii. aggregation.ten known or potential components of the aggregation-attachment pheromone (aap) of the ticks amblyomma hebraeum and a. variegatum, as well as mixtures of these components, extracts of prefed males and live prefed males, were tested as aggregation stimulants. in field assays, laboratory-reared unfed male and female ticks were released 20 cm downwind of co2/pheromone release sites; the numbers of ticks that aggregated at the release sites were recorded after 30 min. in a. variegatum, aggregation w ...19921286609
aride virus, a new ungrouped arbovirus infecting amblyomma loculosum ticks from roseate terns in the seychelles. 19761259597
studies on theileriidae (sporozoa) in tanzania. i. tick transmission of haematoxenus veliferus.haematoxenus veliferus was experimentally transmitted in cattle on two occasions by the tick amblyomma variegatum; transmission was transstadial, from nymph to adult. theileria mutans and cowdria ruminatium were transmitted at the same time. h. veliferus infected at least 15% of red cells in a splenectomized steer and 10% in a normal steer. one experiment on transstadial transmission from larva to nymph was unsuccessful.19761258136
aggregation response of nymphs to pheromone(s) produced by males of the tick amblyomma hebraeum (koch). 19761250397
[the tick amblyomma variegatum (ixodidae), apparently resistant to arsenic, in madagascar]. 19751230882
host and distributional records for the tick amblyomma inornatum (banks) (acarina: ixodidae), with descriptions of the immature stages. 19751223295
arsenic resistance in species of multi-host ticks in the republic of south africa and swaziland.a survey was made in the republic of south africa and swaziland to determine the incidence of arsenic resistance in multi-host ixodid ticks. arsenic resistant strains were found in the following species:- rihipicephalus appendiculatus, rhipicephalus evertsi, rhipicephalus capensis, amblyomma hebraeum and a species of hyalomma, tentatively identified as hyalomma rufipes. strains of haemaphysalis leachi, haemaphysalis silacea and ixodes pilosus were also tested.19751223285
reproduction in ticks (acari: ixodoidea). 4. effects of mechanical and chemical stimulation on oocyte development in amblyomma americanum. 19751172534
studies on the ecology of amblyomma marmoreum koch 1844 (acarnia: ixodidae).in the eastern cape province of south africa, larvae and nymphs of ablyomma marmoreum koch occur in habitats in which there is tree cover and herbaceous ground cover. immatures of a. marmoreum are parasitic on reptiles, birds, and mammals. adults occur only on reptiles. the major host for all stages is the moutain tortoise (geochelone pardalis), and in the home areas of tortoises the density of ticks is high. larvae are active in late summer and autumn. the rate of feeding is temperature-depende ...19751165558
the effect of host species on the engorgement, molting success, and molted weight of the gulf coast tick, amblyomma maculatum koch (acarina: ixodidae). 19751159746
evaluation of co2-baited traps for survey of amblyomma maculatum koch and dermacentor variabilis say (acarina: ixodidae). 19751159725
some aspects of the ecology and lifecycle of amblyomma cajennense (fabricius 1787) in trinidad and their influence on tick control measures.the distribution in trinidad of the tick amblyomma cajennense is defined, and the methods used to determine the boundaries of the infested areas are outlined. the relationships between climate, vegetation and husbandry methods and the distribution of the tick are described. monthly tick collections from specific animals to determine exact seasonal variations were not possible but a study was made of the normal life cycle under laboratory conditions, the results being used to assist in interpreti ...19751124964
a review of the evidence in retrospect for a rickettsial etiology in bullis fever.the authors have presented evidence through work done 25-30 years ago for the rickettsial etiology of bullis fever, a forgotten epidemic which occurred with over a 1,000 cases among world war ii troops in training at camp bullis, texas. rickettsiae were recovered from blood and lymph nodes of patients and from ticks, amblyomma americanum, collected in the area. all patients gave a history of tick-bites. the human and tick strains of rickettsiae, carried in chick embryo culture and in animal pass ...19751103350
[isolation of thogoto virus from amblyomma and boophilus ticks in central africa].a survey has been carried out in the cameroons and the central african republic about the role of the ticks in the ecology of arboviruses in central africa. attempts for virus isolation were made for 12,678 amblyomma variegatum, 3,276 boophilus decoloratus and 1,769 boophilus annulatus, by inoculation into suckling mice. from 968 pools which were inoculated, 20 yielded isolates which were identified to thogoto virus. considering previously published results concerning tick-borne virus isolations ...19761037382
studies on theileriidae (sporozoa) in tanzania. v. preliminary experiments on a new method for infecting ticks with theileria parva and theileria mutans.bovine blood with a high parasitaemia of theileria mutans was injected through the cuticle into fully engorged nymphs of amblyomma variegatum which had recently detached from a rabbit. after moulting, they transmitted this parasite to a susceptible calf. rhipicephalus appendiculatus transmitted theileria parva in the same way, but results were negative with a. variegatum and t. parva. this method of infecting ticks appears promising for the isolation of theileria strains from the field, and migh ...19761006800
tick paralysis in a sheep caused by nymphs of amblyomma variegatum. a preliminary report. 1976988681
comparative observations on some bionomics of japanese ixodid ticks under laboratory cultural conditions.seventeen species of japanese ixodid ticks of the genera amblyomma, boophilus, haemaphysalis, ixodes, and rhipicephalus were reared in the laboratory under almost the same cultural conditions. their major biological characters, such as feeding, molting, oviposition, and hatching, were summarized in tables. there were some differences in these bionomics among the species. the general characteristics common to all the ticks, except amblyomma testudinarium, were as follows. the feeding period in im ...1976980121
accumulating process and concentration ratios of ingested blood meals in larvae and nymphs of ten species of ticks.the accumulating process and concentration ratios of ingested blood meals in the larvae and nymphs of amblyomma testudinarium, haemaphysalis campanulata, h. concinna, h. formosensis, h. hystricis, h. kitaokai, the bisexual and parthenogenetic strains of h. longicornis, h. megaspinosa, and ixodes persulcatus on rabbits and argas japonicus on chickens were comparatively investigated. the larvae and nymphs of the ixodid ticks examined and the larvae of a. japonicus concentrated the ingested blood m ...1976980120
trypanosomes in amblyomma americanum from oklahoma. 1976978370
peripheral nervous system of the ticks, amblyomma tuberculatum marx and argas radiatus railliet (acari: ixodoidea).the peripheral nervous system of an ixodid and an argasid tick include the following components: (1) paired optic, cheliceral and pedipalpal nerves and unpaired stomodeal nerve arising from the pre-esophageal portion of the central nervous system (synganglion); (2) 4 pairs of pedal nerve trunks (each with appendicular and hemal branches) and 4 major pairs of opisthosomal nerves (including the paraspiracular, genital, postlateral-myosomal, and ano-myosomal nerves); and (3) the lateral "sympatheti ...1976978369
field evaluation of arthropod repellent netting as a ground cloth against ticks.tick repellent, wide-mesh net ground cloths were tested against amblyomma americanum (linnaeus) by the co2 bait technique. two repellents, n,n-diethyl-metatoluamide (deet) and 3 acetyl-2-(dimethyl-5-heptenyl)-oxazoladine, and two netting fabrics were compared. all combinations tested provided over 90% protection. the effectiveness of repellent ground cloths against chiggers is discussed.1976961999
use of high-performance liquid chromatography to separate very small quantities of radio-labelled metabolites from biological tissue.in order to study the absorption, metabolism and tissue distribution of chemicals in cattle ticks amblyomma variegatum, a method of separating and recovering metabolites in picogram quantities was required. a high-performance liquid chromatographic system has been investigated for this purpose, using tritium-labelled amitraz and some potential metabolites. separation was carried out on nucleosil 5 mum no2 bonded phase. interchangeable pre-columns were used to extend the range of compounds which ...1977914943
pig production in the solomon islands. ii. diseases and parasites.a study of diseases and parasites of pigs in the solomon islands during 1967 to 1969 indicated that infectious diseases were of little consequence. four arthropod parrasite species were found: one tick, one louse and two tabanids. the tick (amblyomma cyprium cyprium) was extremely rare. fifteen species of helminth parasites were recorded and of these stephanurus dentatus was the most prevalent. all species were identified except a filariid, of which only the microfilariae were seen.1977910298
transmission of a theileria species to cattle by the ixodid tick, amblyomma cohaerens donitz 1909.amblyomma cohaerens nymphs, which had been collected as engorged larvae from african buffalo (syncerus caffer) in the mara region of kenya, transmitted a theilerial parasite to a steer. macroschizonts were detected in the regional lymph node of this steer 12 days after tick application and were subsequently detected for 3 days at low levels of parasitosis. the macroschizonts were large and had numerous nuclei, being morphologically unlike any other theilerial schizonts described previously from ...1977906088
amblyomma inornatum (acarina: ixodidae): natural hosts and laboratory biology. 1977903941
evidence for an assembly pheromone(s) produced by males of the bont tick, amblyomma hebraeum (acarina: ixodidae). 1977903938
the influence of adrenergic agonists and their antagonists on isolated salivary glands of ixodid ticks.various drugs elicit fluid secretion by isolated salivary glands of two species of ixodid ticks (dermacentor andersoni stiles and amblyomma hebraeum koch). among catecholamines, the following order of potency was observed: dopamine, epinine, noradrenaline = adrenaline and isoprenaline. the following drugs, in order of potency, were also agonists on this preparation: ergonovine, ergotamine, 6-hydroxydopamine, apomorphine, phenylephrine, norphenylephrine, beta-phenylethylamine, tyramine, d, l-dopa ...1977891618
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