Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| identification of a calcitonin-like diuretic hormone that functions as an intrinsic modulator of the american lobster, homarus americanus, cardiac neuromuscular system. | in insects, a family of peptides with sequence homology to the vertebrate calcitonins has been implicated in the control of diuresis, a process that includes mixing of the hemolymph. here, we show that a member of the insect calcitonin-like diuretic hormone (cldh) family is present in the american lobster, homarus americanus, serving, at least in part, as a powerful modulator of cardiac output. specifically, during an ongoing est project, a transcript encoding a putative h. americanus cldh precu ... | 0 | 20008368 |
| anomalous contraction of invertebrate striated muscle. | the phenomenon of a band shortening or contraction has been investigated in glycerinated myofibrils of pecten irradians, homarus americanus, cambarus virilis, and limulus polyphemus through the techniques of ultraviolet microbeam inactivation and polarization microscopy. with the former method, it has been shown that these muscles, even though exhibiting the shortening effect, contract in a manner consistent with only the sliding filament model. intrinsic birefringence studies have indicated no ... | 1965 | 4957258 |
| studies on gaffkemia, a bacterial disease of the american lobster, homarus americanus (milne-edwards). | 1965 | 5848793 | |
| gaffkemia, a bacterial disease of the lobster, homarus americanus: effects of the pathogen, gaffkya homari, on the physiology of the host. | 1969 | 5344746 | |
| microscopic localization of cholinesterases in the nervous systems of the lobsters, panulirus argus and homarus americanus. | using acetylthiocholine as substrate, microscopically localizable cholinesterase (che) activity is demonstrated in neural and glial elements of central and peripheral nervous systems of the lobsters, panulirus argus and homarus americanus. moderate to very intense che activity occurs in all synaptic regions of the central ganglia and stomatogastric ganglion, in glial sheaths around neuron somata and peripheral nerve axons, and in cytoplasm of a few nerve cell bodies. axons, identified as motor, ... | 1971 | 18631552 |
| effects of gaffkya homari on glucose, total carbohydrates, and lactic acid of the hemolymph of the lobster (homarus americanus). | 1972 | 5071236 | |
| natural and induced bactericidal activities in the hemolymph of the lobster, homarus americanus: products of hemocyte-plasma interaction. | 1972 | 4561034 | |
| partial characterization of a natural agglutinin in the hemolymph of the lobster, homarus americanus. | 1973 | 4706243 | |
| depletion of glycogen and adenosine triphosphate as major factors in the death of lobsters (homarus americanus) infected with gaffkya homari. | 1973 | 4754747 | |
| the structure of the stomatogastric neuromuscular system in callinectes sapidus, homarus americanus and panulirus argus (decapoda crustacea). | 1974 | 4152406 | |
| a mycosis of the american lobster, homarus americanus, caused by fusarium sp. | 1975 | 1167883 | |
| comparative studies in synaptosome formation: preparation of synaptosomes from the ventral nerve cord of the lobster (homarus americanus). | a flotation method for preparing synaptosomes, previously developed for work with squid nervous tissue, has now been successfully applied to the ventral nerve cord of lobster. perhaps due to the greater content of connective tissue, homogenization of the lobster nerve cord was more difficult than with squid optic lobes and the yield of synaptosomes was lower. the synaptosomes fraction showed a 3.8-fold enrichment of bound acetylcholine relative to the homogenate and was almost 10 times richer in ... | 1976 | 1104081 |
| observations on the feeding mechanism, diet and digestive physiology of histriobdella homari van beneden 1858: an aberrant polychaete symbiotic with north american and european lobsters. | 1. the aberrant annelid histriobdella homari (polychaeta:eunicida) lives in the branchial chambers of the marine lobsters homarus americanus and h. vulgaris where it feeds on the rich microflora of bacteria, blue-green algae and related organisms which grow on the inner surface of the branchial chamber, the setae fringing the edges of the carapace, the gill filaments and, especially, the surfaces and setae of the epipodite plates between the gills. h. homari, therefore, is to be regarded as an e ... | 1976 | 1016663 |
| comparative studies on the structure and aggregative properties of the myosin molecule. i. the structure of the lobster myosin molecule. | myosin purified from the abdominal flexor muscle of the lobster, homarus americanus, has a number average length of 1559 +/- 218 a, a rod like tail 1335 a long and a globular head 225 x 45 a as determined from electron microscopic observations on platinum shadowed preparations. the mass of the molecule was determined to be ca. 486,000 daltons from high speed equilibrium centrifugation studies at neutral and alkaline ph, and by sds-acrylamide gel electrophoresis. both sedimentation equilibrium ce ... | 1976 | 132506 |
| mixed function oxidases and nitroreductases in hepatopancreas of homarus americanus. | 1976 | 5238 | |
| serological grouping of virulent and avirulent strains of the lobster pathogen aerococcus viridans. | virulent strains of aerococcus viridans (formerly gaffkya homari) are the aetiologic agents of gaffkemia, a septicaemic disease of the american lobster (homarus americanus). the virulent and avirulent forms of this bacterium, previously thought to be taxonomically indistinguishable, have been differentiated by serological studies. antisera were produced in rabbits using autoclaved bacteria as antigens. reactions were measured by agglutination tests using microtitre techniques and an antigenic sc ... | 1977 | 874455 |
| genic similarity of american and european species of the lobster homarus. | european lobsters (homarus gammarus) from the norway coast and from the irish sea are examined for electrophoretically detectable genetic variation in seventeen functionally different proteins. forty-one loci encoding these proteins are homologous with loci studied in a previous survey of eight populations of h. americanus. progeny hatched from ovigerous norway females show variation in three enzymes, but mendelian inheritance is confirmed only for triosephosphate isomerase and for one of the ph ... | 1977 | 836910 |
| lobster (homarus americanus) striated muscle myosin. | 1. myosin from the thin-filament regulated flexor muscle of lobster contains 2 moles of each of 2 light chains. 2. the lb 1 light chain of 19,000 daltons which can be removed by dtnb is heavier than the dtnb light chain of chicken. the lb 2 light chain of 17,000 daltons can be removed with urea. 3. on electrophoresis in 8 m urea (ph 8.7) the lb 2 light chain migrates with a mobility similar to that of chicken a2, but the lb 1 migrates significantly faster than any of the chicken light chains. 4. ... | 1977 | 318240 |
| natural and induced bactericidal activities of the hepatopancreas of the american lobster, homarus americanus. | 1978 | 731070 | |
| bacteria induced shell disease of lobsters (homarus americanus). | chitin degrading species of bacteria in the genera pseudomonas, vibrio, and beneckea were cultured from the lesions of lobsters (homarus americanus) with a shell disease. a species of bacterium of the genus vibrio (beneckea) produced necrosis characteristics of shell disease in experimental lobsters when the integument had been damaged prior to inoculation. | 1978 | 633512 |
| organization of native and in vitro-reassembled myosin filaments from lobster tonic muscle. | tonic muscle of the crusher claw of the american lobster (homarus americanus) was investigated with respect to sarcomeric organization and the capacity for self-assembly of extracted myosin for comparison with the same properties of rabbit muscle. native myosin filaments in the lobster muscle are much longer than in rabbit skeletal fibers, and differ further in sarcomeric organization in showing an actin-to-myosin relationship in which two actin filaments are shared between adjacent myosins in a ... | 1981 | 7194522 |
| two hexokinases of homarus americanus (lobster), one having great affinity for mannose and fructose and low affinity for glucose. | two major hexokinases (atp: d-hexose 6-phosphotransferases, ec 2.7.1.1) have been identified in tissues of homarus americanus (lobster) and separated from each other by deae-cellulose ion-exchange chromatography and by polyacrylamide gel electrophoresis. the molecular weight of each, determined by gel filtration, is about 50 000. hexokinase ii, named for its column elution order, resembles hexokinase isozymes i and ii of vertebrates. km values for glucose, mannose and fructose are 0.08, 0.13 and ... | 1981 | 7213758 |
| a preliminary report of vibriosis in cultured american lobsters, homarus americanus. | 1981 | 7217706 | |
| mechanism of lactic acid oxidation catalyzed by lactate dehydrogenase from the tail muscle of homarus americanus. | 1981 | 217308 | |
| stimulation of b cells, but not t cells or thymocytes, by a sialic acid-specific lectin. | haemolymph of the american lobster, homarus americanus, contains several lectins. one of these, lobster agglutinin 1 (lag1) is specific for n-acetylneuraminic acid and agglutinates mouse and human erythrocytes. in addition, this lectin agglutinates peripheral t cells and cortisone-resistant thymocytes, but agglutinates whole thymocytes poorly. because this material is being used to prepare purified populations of cortical thymocytes, and then to study their maturation, it was important to determ ... | 1982 | 7035340 |
| insulin-like peptides in the lobster homarus americanus. i. insulin immunoreactivity. | experiments were conducted to determine if insulin-like peptides are present in the lobster homarus americanus. peptides were found that bind specifically to bovine insulin antibodies in a modified vertebrate radioimmunoassay. extracts of whole hepatopancreas, gut, and hemolymph contained insulin immunoreactivity (iri) concentrations of 67.5, 14.0, and 11.0 ng, respectively, per 700-g lobster. no insulin immunoreactivity was detected in neurosecretory cells of the eyestalk. the highest immunorea ... | 1983 | 6350105 |
| studies on the structure and carbohydrate binding properties of lobster agglutinin 1 (lag1), a sialic acid-binding lectin. | lobster agglutinin 1 (lag 1) was isolated from the hemolymph of the american lobster (homarus americanus) by a sequential combination of ammonium sulfate precipitation, preparative starch block electrophoresis, gel filtration and affinity chromatography on sepharose-fetuin and sepharose-colominic acid columns. two types of protomeric structures with molecular weights of 700 and 500 kilodaltons respectively were isolated. these molecules are composed of noncovalently held subunits with a molecula ... | 1984 | 6333029 |
| 1-14c-n-hexadecane disposition in the spiny lobster, panulirus argus and the american lobster, homarus americanus. | 1-14c-n-hexadecane, a model compound for the non-volatile aliphatic hydrocarbon components of crude oil, was administered by intrapericardial injection to the spiny lobster, panulirus argus, and the clawed or american lobster, homarus americanus. experiments were conducted in florida (spiny lobster) and maine (american lobster). the animals were sacrificed at various times from 0.5 hr to 8 wks after administration of the dose. the tissues and fluids were analyzed for 14c content by digestion or ... | 1986 | 3783435 |
| hemagglutinins and bacterial agglutinins of earthworms. | the biological roles of invertebrate agglutinins have been and remain an unresolved subject of controversy. classical studies on agglutinins, beginning with the pioneer work of noguchi (1903) on limulus polyphemus and homarus americanus have emphasized their hemagglutinating properties, an approach that has been criticized for its lack of biological relevance. while erythrocyte agglutination has proven useful for determining various properties of invertebrate agglutinins, it does not address the ... | 1987 | 3295894 |
| a functional, thioester-containing alpha 2-macroglobulin homologue isolated from the hemolymph of the american lobster (homarus americanus). | an alpha 2-macroglobulin-like protease inhibitor was isolated from the cell-free hemolymph of the american lobster (homarus americanus) by ion-exchange chromatography and gel filtration. whereas the undissociated molecule has a molecular weight of 342,000 as determined by ultracentrifugation studies, under reducing sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the protein has a subunit molecular weight of 180,000. on the basis of this and other evidence, we conclude that the lobster ... | 1987 | 2444589 |
| investigation of american lobster, homarus americanus, for the presence of chlorinated dibenzo-p-dioxins and dibenzofurans. | 1987 | 3440145 | |
| immunochemical and immunocytochemical studies of the crustacean vitellogenesis-inhibiting hormone (vih). | immunochemical investigations, using dot immunobinding assay (dia) and enzyme-linked immunosorbent assay (elisa), and immunocytochemical studies reveal the following new information about crustacean vitellogenesis-inhibiting hormone (vih): (1) the structure of vih is sufficiently different from that of the other sinus gland neuropeptides to allow a selective recognition of vih by polyclonal antibodies. (2) from immunochemical criteria, vih does not seem strictly species specific. the antisera ra ... | 1987 | 3666410 |
| histamine-induced modulation of olfactory receptor neurones in two species of lobster, panulirus argus and homarus americanus. | in two species of lobster, application of the biogenic amine, histamine (ha), to the soma of olfactory receptor cells suppressed both spontaneous and odour-evoked activity, as shown by electrophysiological recording from single cells. the action of ha was graded, reversible, specific to ha, and had a threshold between 0.1 and 1 mumol l-1. ha increased the conductance of the membrane, primarily to chloride ions. the vertebrate ha receptor antagonist, cimetidine, and the nicotinic receptor antagon ... | 1989 | 2809493 |
| histamine directly gates a chloride channel in lobster olfactory receptor neurons. | biogenic amines mediate many types of intercellular communication in multicellular organisms. heretofore, little direct evidence has indicated that biogenic amines produce intracellular responses other than by triggering the enzymatic production of second messengers. our electrophysiological studies of lobster olfactory receptor neurons now reveal that one biogenic amine, histamine, can directly gate an ion channel. the channel responds to histamine concentrations of 1 microm or more, is permeab ... | 1989 | 2479018 |
| structural and functional diversity of copper-metallothioneins from the american lobster homarus americanus. | the role of copper metallothionein (cumt) in copper metabolism and metalloenzyme activation is poorly understood. we have chosen marine crustaceans, in which a direct correlation exists between levels of cu(i)mt and cu(i)-hemocyanin during the molt cycle (engel and brouwer, biol. bull. 173, 239-251, 1987) as unique model systems to study the involvement of mts in metalloprotein activation and degradation. we have isolated three low-molecular weight, cysteine-rich copper proteins from the america ... | 1989 | 2709004 |
| [human somatotropin stimulates the growth of young american lobsters, homarus americanus (crustacea, decapoda)]. | larval and postlarval lobsters, injected with human somatotropin (sth, or growth hormone), grew at a more rapid rate than untreated control animals over succeeding molts. their mean carapace length was significantly longer than that of controls and they were heavier, although the moisture content of treated and control animals was similar. injected sth increased the growth rate of individual animals by 10 to 20%. this is the first evidence for a growth enhancing effect of human somatotropin on a ... | 1989 | 2493311 |
| spectrophotometric and fluorescence studies of the interaction of adenine nucleotides with arginine kinase of homarus americanus. | 1989 | 210171 | |
| american lobster troponin. | troponin was isolated from the abdominal muscle of the american lobster (homarus americanus) by essentially the same method as used for akazara scallop troponin [j. biol. chem. 261, 16749-16754 (1986)]. the thus isolated troponin together with lobster tropomyosin confers high ca2(+)-sensitivity to rabbit reconstituted actomyosin. the troponin consists of components having mr of about 42,000, 32,000, 30,000, and 17,000, but not the mr 52,000-59,000 component previously reported to be present in s ... | 1990 | 2149856 |
| three-dimensional structure of an invertebrate intercellular communicating junction. | gap junctions containing extensive, highly ordered crystalline arrays of hexagonally packed connexons have been isolated from the hepatopancreas of the arthropod, homarus americanus (american lobster). the structure of such junctions has been studied to a resolution of approximately 25 a in three dimensions by electron microscopy of negatively stained specimens. the structure, which has the crystallographic symmetry of the two-sided plane group p6, reveals the connexon as an annular oligomer whi ... | 1991 | 1804280 |
| alpha2-macroglobulin from hemolymph of the freshwater crayfish astacus astacus. | 1. a high mol. wt proteinase inhibitor has been purified from the haemolymph of the freshwater crayfish astacus astacus. 2. the protein is a disulphide-bonded dimer (mr 390,000) of two identical polypeptide chains (mr 185,000). 3. the inhibitor displays a broad specificity and protects trypsin from inhibition by soybean trypsin inhibitor and thus is similar to vertebrate alpha 2-macroglobulin. 4. the alpha 2-macroglobulin-like inhibitor from astacus interacts with bovine trypsin in an equimolar ... | 1991 | 1714366 |
| immunological relationships between neuropeptides from the sinus gland of the lobster homarus americanus, with special references to the vitellogenesis inhibiting hormone and crustacean hyperglycemic hormone. | antisera raised in guinea pigs against four major neuropeptides purified from sinus glands of the lobster, homarus americanus, were used to study the immunological relationships between several sinus gland peptides. on the basis of their behavior in elisa and in absorption procedures, three groups of peptides are defined. two groups may be related to the crustacean hyperglycemic hormone (chh groups); the third one is composed of three immunologically identical peptides and, since one of these pe ... | 1991 | 1711494 |
| the reactivity of edta, copper ion, and copper citrate with metallothioneins isolated from the digestive gland of cadmium-contaminated lobster (homarus americanus). | the reactivity of edta, cu2+, and copper citrate with two metallothionein preparations (mt-1 and mt-2) isolated from the digestive gland of cd-contaminated american lobster (homarus americanus) was studied. under pseudo-first-order conditions, metallothioneins reacted with edta for removal of cd2+ in a multiphasic manner. cadmium(+ii) removal by cu2+ was complex and non-stoichiometric, suggesting different binding sites. rabbit liver metallothionein reacted similarly. cadmium removal from lobste ... | 1991 | 1925524 |
| immunocytochemical localization of the multicatalytic proteinase (proteasome) in crustacean striated muscles. | multicatalytic proteinase (mcp) is thought to play a central role in the processing and turnover of intracellular proteins in eukaryotic cells. immunocytochemistry was used to determine the intracellular distribution of the mcp in the claw muscles of the land crab, gecarcinus lateralis, and the claw and abdominal muscles of the american lobster, homarus americanus. cryosections were stained with an affinity-purified polyclonal antibody to lobster mcp that cross-reacted with the land crab enzyme. ... | 1992 | 1518511 |
| characterization of a g-protein from the mandibular organ of the lobster homarus americanus (nephropidae, decapoda). | 1. gtp-binding activity was found in both calf brain and male lobster mandibular organ (mo). there was approximately two to three times as much binding in the calf brain. 2. the gtp-binding activity could be extracted from the calf brain with sodium cholate, but not from the mos. 3. using adp-ribosylation catalyzed by pertussis toxin, gtp-binding was shown to be the result of the presence of g-protein. in the lobster mo the g-protein alpha subunit has a molecular weight of about 42 kda and may b ... | 1992 | 1395512 |
| isolation of a neuropeptide from locust corpus cardiacum which influences ileal transport. | 1. schistocerca gregaria ion-transport peptide (scg-itp) was isolated from aqueous extracts of the corpus cardiacum by a four-step procedure, utilizing reverse-phase high-performance liquid chromatography for separation and stimulation of a cl(-)-dependent short-circuit current (isc) across locust ilea as the bioassay. 2. scg-itp has an unblocked n terminus and an apparent relative molecular mass of 7700. thirty-one residues (of an estimated 65) were identified by sequence analysis. 3. scg-itp i ... | 1992 | 1487715 |
| comparative brain ontogeny of the crayfish and clawed lobster: implications of direct and larval development. | the freshwater crayfish cherax destructor and the lobster homarus americanus have many similarities including life style, body form, and neural organization. however, the ontogenic history is very different in the two species. the development of cherax is short and direct whereas the development of homarus comprises three pelagic larval stages and takes more than twice as long from extrusion to benthic stages at constant temperature. in order to determine the progression of maturation of the ner ... | 1993 | 8227524 |
| evidence for a common structure for a class of membrane channels. | electron microscopic analysis of gap-junction-like structures isolated from an anthropod (nephrops norvegicus) and composed of a 16-kda polypeptide, show the functional unit to be a star-shaped hexamer of protein arranged around a central channel which runs perpendicular to the plane of the membrane. estimations of the molecular volume carried out on an averaged projection are consistent with a subunit mass of 16-18 kda. fourier transform infrared spectroscopy indicates a high alpha-helical cont ... | 1993 | 7682941 |
| purification and characterization of a cadmium-induced metallothionein from the shore crab carcinus maenas (l.). | two metallothionein variants were purified from the midgut gland of crabs (carcinus maenas) exposed to a high cadmium concentration (2 p.p.m.). one of the variants was purified from crabs exposed to a low cadmium concentration (0.5 p.p.m.). the purification method involved acetone precipitation, gel filtration and reversed-phase h.p.l.c. the complete amino acid sequences of both variants have been elucidated by m.s. and automated sequence analysis on s-methylated proteins or fragments produced b ... | 1994 | 8110201 |
| effect of cooking on the concentration of toxins associated with paralytic shellfish poison in lobster hepatopancreas. | the hepatopancreases from lobsters (homarus americanus) obtained from two locations in eastern canada (gaspé and bay of fundy) were analysed for paralytic shellfish poisons (psp) before and after the shellfish were cooked by boiling or steaming. forty-five lobsters from each location were divided into three groups of 15. two of the groups were boiled or steamed while the third was uncooked for comparison purposes. the hepatopancreases of all lobsters were individually analysed for total psp toxi ... | 1994 | 9237337 |
| quantitative determination and distribution of the myotropic neuropeptide orcokinin in the nervous system of astacidean crustaceans. | for quantitative determinations of orcokinin, an indirect, noncompetitive sandwich elisa was developed. this elisa is highly specific for orcokinin and the detection limit is 1 fmol. in three astacidean species (orconectes limosus, homarus americanus, and astacus astacus) orcokinin immunoreactivity (ok-ir) was measurable in all parts of the nervous system. upon normalization to the protein content of the tissue (pmol/mg protein), concentrations were shown to be in the same range in all three spe ... | 1994 | 7937311 |
| molecular cloning and sequence analysis of a cdna encoding a molt-inhibiting hormone-like neuropeptide from the white shrimp penaeus vannamei. | the x-organ of the eyestalk in crustaceans is a source of neurosecretory peptides. total rna was isolated from x-organs of the white shrimp penaeus vannamei and used to clone a cdna encoding a molt-inhibiting hormone-like (mih-like) neuropeptide by the 3' and 5' rapid amplification of cdna ends (race) method. sequence analysis of the 470-bp cdna reveals a 306-bp open reading frame and a 164-bp 3' untranslated region. the deduced polypeptide consists of a 72-amino acid mature peptide and a 30-ami ... | 1994 | 8055060 |
| neuropeptides and related nucleic acid sequences detected in peneid shrimps by immunohistochemistry and molecular hybridizations. | the neurosecretory cells in the eyestalks of penaeus indicus and p. vannamei were studied by immunocytochemistry using polyclonal antisera raised against purified homarus americanus neuropeptides. cross-reactions between two h. americanus anti-crustacean hyperglycemic hormone antisera and penaeus neurosecretory material were observed. the specific anti-vitellogenesis inhibiting hormone antiserum only showed an immunological reaction in the nervous tract and the sinus gland of penaeus, suggesting ... | 1994 | 8072696 |
| cu(ii) coordination in arthropod and mollusk green half-methemocyanins analyzed by electron spin-echo envelope modulation spectroscopy. | hemocyanin (hc) is a dinuclear copper protein that binds oxygen reversibly. the structure of the cu(ii) site in a derivative of hemocyanin known as green half-met (ghm) has been analyzed using the pulsed epr technique of electron spin-echo envelope modulation (eseem) spectroscopy. the derivative, prepared by treating the native protein with nitrite at low ph, contains a mixed-valent binuclear copper center. it was shown through chemical assays and the ligand exchange reaction products identified ... | 1995 | 7849010 |
| agglutination of streptococcus suis by sialic acid-binding lectins. | the 35 streptococcus suis capsular-type reference strains as well as 45 field strains of type 2 were tested with sialic acid-binding lectins from sambucus nigra (sna i), triticum vulgaris, maackia amurensis, homarus americanus, and limax flavus. only types 1, 1/2, 2, 14, 15, and 16 agglutinated with sna i and/or the t. vulgaris lectin. all field strains agglutinated only with sna i. reaction with sna i was probably due to the sialic acid moiety since it disappeared after sialidase treatment. the ... | 1995 | 7559986 |
| a novel ef-hand ca(2+)-binding protein from abdominal muscle of crustaceans with similarity to calcyphosine from dog thyroidea. | the amino acid sequence of a novel ef-hand ca(2+)-binding protein from the abdominal muscle of the crayfish, orconectes limosus, has been elucidated by tandem mass spectrometry and automated edman degradation. the name ccbp-23 (23-kda crustacean ca(2+)-binding protein) is proposed. the protein can also exist as a disulfide-linked homodimer. the sequence of the monomeric form spans 200 residues with an acetylated n-terminal ser and reveals four ef-hand domains. the 174-mass-unit difference betwee ... | 1995 | 7851448 |
| molecular cloning and sequencing of two cdnas encoding cathepsin l-related cysteine proteinases in the nervous system and in the stomach of the norway lobster (nephrops norvegicus). | two cathepsin l-related cysteine proteinase molecular clones were isolated from cdna libraries of mrna from the eyestalk nervous system and the stomach of nephrops norvegicus and sequenced. the cdna from nervous system was first obtained by screening an eyestalk cdna library with an oligonucleotide whose sequence derived from the amino acid sequence of a peptide isolated previously and subsequently with the 5' end of the longest cdna probe thus obtained. several clones were isolated and analysed ... | 1995 | 7613761 |
| a gelsolin-related protein from lobster muscle: cloning, sequence analysis and expression. | the tail muscle of the lobster homarus americanus contains an actin-binding protein with an apparent molecular mass of 105 kda determined by sds/page and gelsolin-like properties. we isolated this protein and peptide sequences were obtained after limited proteolysis with chymotrypsin. a tail-muscle-specific cdna library was constructed in a lambda expression vector and a full-length clone was obtained by screening with a polyclonal anti-(crustacean gelsolin) antibody. the cdna insert of approx. ... | 1995 | 7848275 |
| complete primary structure of the molt-inhibiting hormone (mih) of the mexican crayfish procambarus bouvieri (ortmann). | the amino acid sequence of mih was elucidated by means of digestions with specific proteases, manual edman degradation, and mass spectrometry. mih consists of a 72-residue peptide chain (molecular mass 8322 da) with six cysteines forming three disulfide bridges that connect residues 7-43, 23-39, and 26-52. it has blocked n- and c-termini and lacks tryptophan, histidine, and methionine. mih shows striking similarity to the crustacean hyperglycemic hormone (chh) isomorphs of procambarus bouvieri ( ... | 1996 | 8735961 |
| a lobster cysteine protease with immunoreactivity and activities of calcitonin and cgrp. | the high concentrations of molecules immunologically related to salmon calcitonin (ct) and/or to human calcitonin gene-related peptide (cgrp) in the oesophagus of the norway lobster nephrops norvegicus have been examined. in the present study. we report the purification of these molecules by means of a specific radioimmunoassay for calcitonin and calcitonin gene related peptide. the immunoreactive molecules were tested for their functional similarities with ct and cgrp. this was investigated by ... | 1996 | 9033842 |
| characterization of microsatellite markers in homarus (crustacea, decapoda). | three variable microsatellite loci have been isolated from the american lobster, homarus americanus. in a population sample from the gulf of maine, the effective numbers of alleles (ne) for the two most variable loci were 16.33 and 13.19, respectively. reduced variability at all three loci was seen in the european lobster, h. gammarus, for which the maximum ne was 4.00. the reduction in variability in h. gammarus is consistent with a bottleneck event. inheritance analysis using h. americanus dem ... | 1996 | 8817929 |
| the lobster parasite anophryoides haemophila (scuticociliatida: orchitophryidae): nuclear 18s rdna sequence, phylogeny and detection using oligonucleotide primers. | we have determined the nucleotide sequence of the nuclear gene encoding small-subunit ribosomal ribonucleic acid of the ciliate anophryoides haemophila, a parasite of the american lobster homarus americanus. the gene is 1763 bp in length, and has a guanosine-plus-cytosine content of 43.9%. inferred phylogenetic frameworks strongly support the monophyly of the scuticociliates, and suggest that order scuticociliatida should be elevated to at least subclass rank. oligonucleotide probes based on a. ... | 1996 | 8768439 |
| effects of formalin, chloramine-t, and low salinity dip on the behavior and hemolymph biochemistry of the american lobster. | the purpose of this research was to investigate the salinity and formalin sensitivity of a ciliate parasite (anophryoides haemophila) of the american lobster (homarus americanus), and to examine the target-animal (lobster) safety of chemical-bath treatments involving low salinity, formalin, or chloramine-t that could be used to control this parasite in lobster pounds. "bumper car" disease, caused by an. haemophila, is an important concern to lobster pound operators in eastern north america, beca ... | 1996 | 9111691 |
| primary structures of decapod crustacean metallothioneins with special emphasis on freshwater and semi-terrestrial species. | cadmium injections induced only a single form of metallothionein (mt) in the midgut gland of potamon potamios, whereas the same treatment induced two isoforms in astacus astacus. the only difference between the two latter isoforms was that one had an extra n-terminal methionine residue. mt from p. potamios showed structural differences from other decapod crustacean mts. it contained a gly-thr motif at positions 8 and 8a, which had previously been found only in certain vertebrate and molluscan mt ... | 1996 | 8921011 |
| the o-diphenol oxidase activity of arthropod hemocyanin. | arthropod hemocyanin (isolated from the crab carcinus maenas and the lobster homarus americanus) is usually referred to as an oxygen transport-storage protein. the protein, however, also catalyses with low efficiency the oxidation of o-diphenol to quinone, similarly to tyrosinase (monophenol, o-diphenol:oxygen oxidoreductase). the enzymatic parameters of hemocyanin are affected by the aggregation state of the protein; namely v(max) exhibited by a dissociated subunit is one order of magnitude gre ... | 1996 | 8617365 |
| distribution of centrifugal neurons targeting the soma clusters of the olfactory midbrain among decapod crustaceans. | to determine the distribution of two systems of centrifugal neurons innervating the soma clusters of the olfactory midbrain across decapod crustaceans, brains of the following nine species comprising most infraorders were immunostained with antibodies against dopamine and the neuropeptides substance p and fmrfamide: macrobrachium rosenbergii, homarus americanus, cherax destructor, orconectes limosus, procambarus clarkii, astacus leptodactylus, carcinus maenas, eriocheir sinensis and pagurus bern ... | 1997 | 9106436 |
| lethality of pyrethrins to larvae and postlarvae of the american lobster (homarus americanus). | pesticide formulations containing pyrethrins are being used to treat salmonids for infestations of the copepod parasites lepeophtherius salmonis and caligus elongatus (sea lice). the acute lethality of one such formulation to four larval stages of the american lobster (homarus americanus), a species of significant economic importance in eastern canada, was determined. the formulation tested contained 0.06% pyrethrins and 0.6% piperonyl butoxide (a synergist). stage i larvae (48-h lc50 = 4.42 mic ... | 1997 | 9417857 |
| distribution and regulation of esterases that hydrolyze methyl farnesoate in homarus americanus and other crustaceans. | ester hydrolysis of methyl farnesoate (mf) by crustacean tissue homogenates was measured using the substrate [3h]mf in a radiochemical partition assay. tissues were obtained from the lobster homarus americanus, penaeid shrimp sicyonia ingentis, thalanassid shrimp callianassa californiensis, sand crab emerita analoga, and spider crab pugettia producta. the greatest specific activities were recovered from the hepatopancreas (239 to 11,500 pmol mf/min-mg total protein). hepatopancreatic homogenates ... | 1997 | 9126466 |
| distribution of presumptive chemosensory afferents with fmrfamide- or substance p-like immunoreactivity in decapod crustaceans. | in five species of decapod crustaceans--cherax destructor (crayfish), carcinus maenas (crab), homarus americanus (clawed lobster), eriocheir sinensis (crab), macrobrachium rosenbergii (shrimp)--immunocytochemical stainings revealed the presence of sensory afferents with fmrfamide-like immunoreactivity in the central nervous system. these afferents were extremely thin, very numerous, and innervated all sensory neuropils except the optic and olfactory lobes. in their target neuropils they gave ris ... | 1997 | 9037486 |
| catalytic properties of lactate dehydrogenase in homarus americanus. | 1997 | 20049 | |
| amines and peptides in the brain of the american lobster: immunocytochemical localization patterns and implications for brain function. | the distributions of serotonin- (5ht-), substance p- (sp-), small cardioactive peptideb- (scpb-), and histamine- (ha-) like immunoreactivities were examined in the adult lobster supraesophageal ganglion. vibratome sections were labeled using avidin-biotin-peroxidase immunocytochemical methods. the localization patterns for each substance were assessed in 21 regions within the median protocerebrum, deutocerebrum, and tritocerebrum. each immunoreactivity has a unique distribution within the brain; ... | 1997 | 9042786 |
| overview of "bumper car" disease--impact on the north american lobster fishery. | recent (1993) landings of american lobsters (homarus americanus) were valued at $294 million (can.) in canada and $213 million (can.) in the united states. however, post-harvest losses are estimated at $50-75 million (10-15%) annually. the lobster fishery is one of the few remaining viable traditional fisheries in eastern north america. "bumper car" disease of lobsters, caused by the scuticociliate anophryoides haemophila, can cause significant losses in coldwater impoundments. apparently epidem ... | 1997 | 9088987 |
| localization of a crustacean hyperglycemic hormone-like immunoreactivity in the neuroendocrine system of euscorpius carpathicus (l.) (scorpionida, chactidae). | the neuroendocrine system of euscorpius carpathicus was immunohistochemically localized using a polyclonal antiserum raised against a purified homarus americanus crustacean hyperglycemic hormone (hoa-chha). there were cross-reactions in e. carpathicus procerebral and subesophageal neurosecretory cells, neurohemal organs, and intra- and extraganglionic neurosecretory tracts. among the neurohemal structures, the kwartirnikov's organ, the tropfenkomplex, and the coxal disc reacted strongly. in eusc ... | 1997 | 9204365 |
| an ecdysteroid-responsive gene in a lobster - a potential crustacean member of the steroid hormone receptor superfamily. | the role of ecdysteroids in modulating exoskeletal growth during the moult cycle of crustacea has been well described. however, little is known about the action of ecdysteroids at the level of gene transcription and regulation in crustacea. this paper reports the cloning of an ecdysteroid responsive gene, hhr3, a potential manduca sexta mhr3 homologue in the american lobster, homarus americanus. levels of hhr3 expression are up-regulated in response to in vivo injections of premoult concentratio ... | 1997 | 9409780 |
| identification of nitric oxide-sensitive and -insensitive forms of cytoplasmic guanylate cyclase. | cytoplasmic, nitric oxide-activated guanylate cyclases are expressed in many regions of the mammalian brain and are thought to participate in functions as diverse as synaptogenesis and long-term potentiation. in this study, we have characterized cytoplasmic guanylate cyclases in the nervous system of an invertebrate, the american lobster. cytoplasmic cyclase specific activity is higher in lobster nerve cord than in any other lobster tissue tested, and considerably higher than in typical rat tiss ... | 1997 | 9326294 |
| cloning of tropomyosins from lobster (homarus americanus) striated muscles: fast and slow isoforms may be generated from the same transcript. | complementary dnas encoding fibre-type-specific isoforms of tropomyosin (tm) have been isolated from lobster (homarus americanus) striated muscle expression libraries made from poly(a)+ rna purified from deep abdominal (fast-type) and crusher-claw closer (slow-type) muscles. a cdna of slow-muscle tm (stm1), containing a complete open reading frame (orf) and portions of the 5' and 3' untranslated regions (utrs), encodes a protein of 284 amino acid residues with a predicted mass of 32,950, assumin ... | 1998 | 9536438 |
| demonstration of a cell-specific isomerization of invertebrate neuropeptides. | neurohemal organs of the lobster homarus americanus contain isoforms of the crustacean hyperglycemic hormone, which differ by the third amino acid (phenylalanyl) residue that is either in the l- or in the d-configuration. polyclonal antisera have been raised in rabbit against synthetic octapeptides with the sequence corresponding to the n-terminal part of the l- or d-phenylalanine-containing isoforms. their specificity was shown by immunoassays, indicating that they discriminate the isoforms of ... | 1998 | 9483547 |
| elucidation of the amino acid sequence of a crustacean hyperglycaemic hormone from the spiny lobster, jasus lalandii. | we have isolated a peptide from extracts of sinus glands of jasus lalandii, a south african spiny lobster, by high-performance liquid chromatography (hplc) and identified it as crustacean hyperglycaemic hormone (chh) by (i) a conspecific bioassay measuring glucose elevation in the haemolymph and (ii) an immunoassay using an antiserum raised against chh of the american lobster. the j. lalandii peptide has a free n-terminus as evidenced by sequencing the first 30 amino acid residues of the intact ... | 1998 | 9703969 |
| sequential developmental acquisition of neuromodulatory inputs to a central pattern-generating network. | the activity of the adult stomatogastric ganglion (stg) depends on a large number of aminergic and peptidergic modulatory inputs. our aim is to understand the role of these modulatory inputs in the development of the central pattern-generating networks of the stg. therefore, we analyze the developmental and adult expressions of three neuropeptides in the stomatogastric nervous system of the lobsters homarus americanus and homarus gammarus by using wholemount immunocytochemistry and confocal micr ... | 1999 | 10340510 |
| sequential developmental acquisition of cotransmitters in identified sensory neurons of the stomatogastric nervous system of the lobsters, homarus americanus and homarus gammarus. | we studied the developmental acquisition of three of the cotransmitters found in the gastropyloric receptor (gpr) neurons of the stomatogastric nervous systems of the lobsters homarus americanus and homarus gammarus. by using wholemount immunocytochemistry and confocal microscopy, we examined the distribution of serotonin-like, allatostatin-like, and flrf(nh2)-like immunoreactivities within the stomatogastric nervous system of embryonic, larval, juvenile, and adult animals. the gpr neurons are p ... | 1999 | 10340509 |
| the lethality of salmosan (azamethiphos) to american lobster (homarus americanus) larvae, postlarvae, and adults. | the pesticide formulation salmosan (47.5% w/w azamethiphos) is currently registered for use, in canada, to treat salmonids for infestations of the copepod parasites, lepeophtheirus salmonis and caligus elongatus (sea lice). determination was made of the acute lethality of this product to the three larval stages, the first postlarval stage, and the adult of the american lobster (homarus americanus), a species of significant economic importance in eastern canada. the 48-h lc50 (as azamethiphos) is ... | 1999 | 10375419 |
| a comparative immunocytochemical study of the hyperglycaemic, moult-inhibiting and vitellogenesis-inhibiting neurohormone family in three species of decapod crustacea. | eyestalks of the palinuran species jasus lalandii and panulirus homarus, and the brachyuran species carcinus maenas, were examined with antisera raised against purified crustacean hyperglycaemic hormone (chh) of the astacidean species homarus americanus and procambarus bouvieri, as well as the brachyuran species cancer pagurus. other antisera used in this investigation were raised against purified moult-inhibiting hormone (mih) of c. pagurus and vitellogenesis-inhibiting hormone (vih) of h. amer ... | 1999 | 9931363 |
| interpopulation and context-related differences in responses of a marine gastropod to predation risk. | we conducted laboratory experiments to investigate interpopulation differences in the behavioural responses of the whelk buccinum undatum to the predatory lobster homarus americanus and the asteroid leptasterias polaris, both in the absence and presence of feeding opportunities. whelks from three populations in the eastern north atlantic (1) responded to lobsters by displaying avoidance behaviours (burrowing in the sediments or retreating inside their shell), (2) responded to asteroids by displa ... | 1999 | 10202105 |
| subunit composition and n-terminal analysis of arthropod hemocyanins. | fourteen structural subunits of hemocyanins from two different infraorders of crustacea, brachyura (maia squinado, carcinus maenas) and astacidea (homarus americanus) were isolated and characterized. n-terminal amino acid sequences were determined and compared with known sequences of crustacean and cheliceratan hemocyanins. relationships between the investigated polypeptide chains were established. the results demonstrate that the degree of identity, calculated from the amino terminal sequences, ... | 1999 | 10327595 |
| allatostatin-like immunoreactivity in the stomatogastric nervous system and the pericardial organs of the crab cancer pagurus, the lobster homarus americanus, and the crayfish cherax destructor and procambarus clarkii. | the distribution of allatostatin (ast)-like immunoreactivity was studied in the stomatogastric nervous system (stns) and the neurosecretory pericardial organs (po) of four decapod crustacean species by using wholemount immunocytochemical techniques and confocal microscopy. ast-like immunoreactivity was found within the stns of all four species; its distribution in each was unique. in all four species, ast-like immunoreactivity was present in the paired commissural ganglia (cog), in the esophagea ... | 1999 | 10075445 |
| studies on proteins in post-ecdysial nymphal cuticle of locust, locusta migratoria, and cockroach, blaberus craniifer. | proteins were extracted from the cuticle of mid-instar nymphs of locusts, locusta migratoria, and cockroaches, blaberus craniifer. seven proteins were purified from the locust extract and five from the cockroach extract, and their amino acid sequences were determined. polyacrylamide gel electrophoresis indicates that the proteins are present only in the post-ecdysially deposited layer of the nymphal cuticles. one of the locust and one of the cockroach nymphal proteins contain a 68-residue motif, ... | 2000 | 10844249 |
| tailflick escape behavior in larval and juvenile lobsters (homarus americanus) and crayfish (cherax destructor). | we examined the escape behavior of larvae and postlarvae of the american lobster (homarus americanus) and of adult immature (stage adi) crayfish (cherax destructor). responses to standardized water jet stimuli delivered through a pipette were observed and analyzed. lobster larvae did not respond to stimuli within 60 ms, indicating that they do not have functional giant fibers. the first movement by lobster larvae in response to water jet stimuli was a hyperextension of the abdomen. larval escape ... | 2000 | 10897445 |
| synaptic neuropil in nerves of the crustacean stomatogastric nervous system: an immunocytochemical and electron microscopical study. | patches of peptide-immunoreactive varicosities have been found in nerves of the stomatogastric nervous system (stns) of decapod crustaceans. in the present study, these patches were examined in detail in the stomatogastric nerve (stn) and in the superior oesophageal nerve (son) of the crayfish cherax destructor by using whole-mount immunocytochemical techniques combined with confocal microscopy and, in addition, electron microscopy. double-labeling experiments with antibodies generated against t ... | 2000 | 10754509 |
| five crustacean hyperglycemic family hormones of penaeus monodon: complementary dna sequence and identification in single sinus glands by electrospray ionization-fourier transform mass spectrometry. | five novel neuropeptides, designated pm-sgp-i to -v, of the crustacean hyperglycemic hormone (chh) family have been identified from the giant tiger prawn penaeus monodon by isolation of the preprohormone genes from an eyestalk complementary dna library. on the basis of sequence similarity, the encoded peptides have been classified as chh-like type i hormones, which include all known chhs and the molt-inhibiting hormone (mih) of the lobster homarus americanus. consistent with chh type i preprohor ... | 2000 | 10804243 |
| lobster gaba receptor subunit expressed in neural tissues. | a cdna encoding an ionotropic gamma-aminobutyric acid (gaba) receptor subunit was isolated from a lobster (homarus americanus) cdna library. a longer version of this cdna, containing a 108-bp insert, was also detected. the two cdnas are predicted to encode alternatively spliced proteins of 485 and 521 amino acids, respectively. the sequences were most similar to the drosophila rdl (resistance to dieldrin) gaba subunit with 54% identity, and 30-35% identity with vertebrate ionotropic gaba recepto ... | 2000 | 10679793 |
| a long-term mesocosm study on the settlement and survival of juvenile european lobster homarus gammarus l. in four natural substrata. | to date, the natural substratum preferences of early benthic phase (ebp) european lobsters (homarus gammarus) remain largely unknown. this study utilised a large scale mesocosm experiment to determine if the animal favours cobble ground, similar to its american counterpart (homarus americanus), or has other substratum preferences. postlarvae were provided with the choice of settling on four natural substrata: sand, coralline algae, mussel shell and cobble. over a nine month period, the number an ... | 2000 | 10817827 |
| transport of (22)na(+) and( 45)ca(2+) by xenopus laevis oocytes expressing mrna from lobster hepatopancreas. | this paper describes the development of a functional assay system to express crustacean epithelial electrogenic 2na(+)/1h(+) antiporters in xenopus laevis oocytes. subsequent publications will use this assay method to establish nucleotide and amino acid sequence information about this transporter by functionally screening an hepatopancreatic cdna library. in this method, oocytes were injected with hepatopancreatic mrna (50 ng) isolated from homarus americanus, while control oocytes received inje ... | 2001 | 11550182 |
| quadrupole ion storage tandem mass spectrometry and high-resolution mass spectrometry: complementary application in the measurement of 2,3,7,8-chlorine substituted dibenzo-p-dioxins and dibenzofurans in us foods. | the us food and drug administration has simultaneously utilized both high-resolution mass spectrometry (hrms) and quadrupole ion storage tandem mass spectrometry (qistms) in the measurement of polychlorinated dibenzo-p-dioxins/dibenzofurans (pcdd/fs) in 147 food samples collected in 1998 and 1999 in the us. in 1998, 20 egg samples, six scallop, 10 blue crab, eight american lobster, 10 pollack, 15 striped bass, five rockfish, 10 crawfish, seven aqua-cultured and 13 wild-caught salmon, along with ... | 2001 | 11372820 |
| a new insight into metallothionein (mt) classification and evolution. the in vivo and in vitro metal binding features of homarus americanus recombinant mt. | we report the synthesis and characterization of a homarus americanus mt-cdna (mth) through retrotranscription of mth-mrna from metal-injected lobsters. heterologous escherichia coli expression in zinc- and copper-supplemented medium was achieved for mth, the two domains betabetamth and betaalphamth and three site-directed mutants, betabetac9h, betaalphac37h, and betaalphae31c/t34c. the in vivo conformed metal complexes and the in vitro substituted cadmium aggregates were characterized. major sto ... | 2001 | 11413132 |
| estimated prevalence of aerococcus viridans and anophryoides haemophila in american lobsters homarus americanus freshly captured in the waters of prince edward island, canada. | the canadian lobster industry holds lobsters homarus americanus in captivity for various periods to supply markets with live product year-round. mortality during holding results in considerable losses, estimated at 10 to 15 % yr(-1) by the industry. this study examined the prevalence of anophryoides haemophila and aerococcus viridans, causative agents of 'bumper car' disease and gaffkemia, respectively, in lobsters freshly captured in the waters of prince edward island during the spring and fall ... | 2001 | 11710558 |
| gonad-inhibiting hormone of the norway lobster (nephrops norvegicus): cdna cloning, expression, recombinant protein production, and immunolocalization. | the gonad-inhibiting hormone (gih) belongs to a neuropeptide family synthesized and released in a neurohemal complex of crustacean eyestalks. the gih is involved in gonad maturation and plays a more complex role in the control of reproduction and molting. with a combination of reverse transcription-polymerase chain reaction (rt-pcr) and rapid amplification of cdna ends approaches we determined the cdna sequence of the norway lobster nephrops norvegicus prepro gih. the open reading frame of 339 b ... | 2002 | 11891050 |
| structure of the (113)cd(3)beta domains from homarus americanus metallothionein-1: hydrogen bonding and solvent accessibility of sulfur atoms. | the three-dimensional structures of the isolated cd(3)beta domains from homarus americanus metallothionein have been determined by nmr methods in order to establish a set of beta-domain structures for comparative analysis. first, it was determined that the cd-cysteine connectivities forming the cd(3)s(9) metal center were identical to those observed for the beta(n) domain in the native holoprotein. time- and temperature-dependence studies of the (113)cd and (1)h 1d-nmr spectra indicated that the ... | 2002 | 12203008 |
| two sniffing strategies in palinurid lobsters. | most studies of lobster chemoreception have focused on the model systems of panulirus argus (palinuridae) and homarus americanus (nephropidae). we compare antennule morphology across lobsters and conduct the first kinematic study of antennule flicking in a palinurid species other than p. argus. high-speed video analysis shows that palinurus elephas flicks at a rate more than an order of magnitude higher than in p. argus. however, both species flick their antennular flagella at a reynolds number ... | 2002 | 12432011 |
| thermal acclimation and stress in the american lobster, homarus americanus: equivalent temperature shifts elicit unique gene expression patterns for molecular chaperones and polyubiquitin. | using homologous molecular probes, we examined the influence of equivalent temperature shifts on the in vivo expression of genes coding for a constitutive heat shock protein (hsc70), heat shock proteins (hsps) (hsp70 and hsp90), and polyubiquitin, after acclimation in the american lobster, homarus americanus. we acclimated sibling, intermolt, juvenile male lobsters to thermal regimes experienced during overwintering conditions (0.4 +/- 0.3 degrees c), and to ambient pacific ocean temperatures (1 ... | 2002 | 11892992 |
| putative neurohemal release zones in the stomatogastric nervous system of decapod crustaceans. | the stomatogastric nervous system (stns) of decapod crustaceans has long been used to study the modulation of small neural circuits. profiles in the sheath of the nerves and ganglia of the stns, which contain only dense-core vesicles, have been described in electron microscopical studies (friend [1976] cell tissue res. 175:369-380; kilman and marder [1997] soc neurosci abstr. 23:477; skiebe and ganeshina [2000] j comp neurol 420:373-397). these profiles resemble those found in neurohemal organs ... | 2002 | 12378588 |
| development, characterisation, inheritance, and cross-species utility of american lobster (homarus americanus) microsatellite and mtdna pcr-rflp markers. | effective management of exploited species demands contemporary knowledge of population structure and mating patterns. genetic markers can prove useful in providing this knowledge. despite its commercial importance, genetic markers for american lobster (homarus americanus) are limited. we developed 12 tetra- and 1 trinucleotide microsatellite loci for american lobster that exhibit little stuttering after pcr amplification. gene diversity of these loci ranged from 0.516 to 0.929. a four-locus mult ... | 2003 | 12669797 |