| production of chitin and bioactive materials from black tiger shrimp (penaeus monodon) shell waste by the treatment of bacterial protease cocktail. | the main objective of this study was to obtain chitin in pure form from a new crustacean waste material for industrial applications. black tiger shrimp shell wastes are a rich source of protein and valuable bioactive carbohydrate polymers such as chitin. after removal of carotenoid, black tiger shrimp shell wastes (btshws) were treated with chemicals and protease enzyme to extract chitin. box-behnken response surface methodology was applied to optimize the deproteinization process to obtain chit ... | 2015 | 28324551 |
| determination of the persistence of dimetridazole, metronidazole and ronidazole residues in black tiger shrimp (penaeus monodon) tissue and their stability during cooking. | the depletion of three banned nitroimidazole drugs - dimetridazole (dmz), metronidazole (mnz) and ronidazole (rnz) - was investigated in black tiger shrimp (penaeus monodon) following in-water medication. the highest concentrations of residues were measured immediately after the 24-h immersion (d0). at this time, mnz and mnz-oh residues were measured in shrimp tissue samples at concentrations ranging from 361 to 4189 and from 0.28 to 6.6 μg kg(-1), respectively. dmz and its metabolites hmmni ran ... | 2015 | 25372355 |
| clinical and immunochemical profiles of food challenge proven or anaphylactic shrimp allergy in tropical singapore. | shellfish allergy in singapore is highly prevalent, and shrimp allergy is the most common. | 2015 | 25257922 |
| demonstration of a very inexpensive, turbidimetric, real-time, rt-lamp detection platform using shrimp laem-singh virus (lsnv) as a model. | rapid and accurate detection of pathogens under field laboratory conditions is necessary for effective control of veterinary pathogens. here we describe a prototype, portable, pathogen detection device developed for single tube, real-time, reverse transcription, loop-mediated isothermal amplification (rt-lamp) using laem-singh virus (lsnv) as a model. lsnv is an rna virus and a component cause of growth retardation in black tiger shrimp. we chose its rna-dependent rna polymerase (rdrp) gene as t ... | 2014 | 25255231 |
| a risk assessment of heavy metal concentrations in fish and an invertebrate from the gulf of antalya. | this study was conducted to determine the levels of cd, cu, zn and pb in the red mullet (mullus barbatus), grey mullet (mugil cephalus) and green tiger prawn (panaeus semisulcatus) species caught in the gulf of antalya and to assess the health risks associated with their consumption. the average values (mg/kg d.w.) were calculated to be pb (0.29 ± 0.14), cd (0.02 ± 0.03), zn (5.64 ± 1.58) and cu (1.64 ± 1.02) in m. barbatus; pb (0.22 ± 0.12), cd (0.02 ± 0.01), zn (7.66 ± 2.29) and cu (1.33 ± 0.8 ... | 2014 | 25216933 |
| quantitative analysis of low-density snp data for parentage assignment and estimation of family contributions to pooled samples. | while much attention has focused on the development of high-density single nucleotide polymorphism (snp) assays, the costs of developing and running low-density assays have fallen dramatically. this makes it feasible to develop and apply snp assays for agricultural species beyond the major livestock species. although low-cost low-density assays may not have the accuracy of the high-density assays widely used in human and livestock species, we show that when combined with statistical analysis app ... | 2014 | 25183297 |
| co-solvent selection for supercritical fluid extraction of astaxanthin and other carotenoids from penaeus monodon waste. | in recent years, astaxanthin is claimed to have a 10 times higher antioxidant activity than that of other carotenoids such as lutein, zeaxanthin, canthaxanthin, and β-carotene; the antioxidant activity of astaxanthin is 100 times higher than that of α-tocopherol. penaeus monodon (tiger shrimp) is the largest commercially available shrimp species and its waste is a rich source of carotenoids such as astaxanthin and its esters. the efficient and environment-friendly recovery of astaxanthins was ac ... | 2014 | 25007745 |
| high salinity induced expression profiling of differentially expressed genes in shrimp (penaeus monodon). | four suppression subtractive hybridization (ssh) cdna libraries were constructed to identify differentially expressed salinity stress responsive genes of black tiger shrimp, penaeus monodon exposed to high (55 ppt) salinity conditions. one each of the forward and reverse ssh cdna libraries were developed from the gill and gut tissues of shrimp and clones having inserts larger than 300 bp were unidirectionally sequenced. based on the sequence homology search, the identified genes were categorized ... | 2014 | 24973887 |
| proteomic analysis of ovarian proteins and characterization of thymosin-β and rac-gtpase activating protein 1 of the giant tiger shrimp penaeus monodon. | cellular proteomics of total proteins in ovaries of domesticated and wild giant tiger shrimp (penaeus monodon) were examined using gelc-ms/ms. in total, 1638 proteins matched those previously deposited in databases and 1253 (76.50%) of these significantly matched known proteins. several reproduction-related proteins (e.g. cdc2, cyclin b, cdc25, 14-3-3, thymosin-β and rac-gtpase activating protein 1) were identified. in addition, the full-length cdna of p. monodon thymosin-β (pmtmsb; 1084 bp with ... | 2014 | 24946223 |
| molecular characterization of a cdna encoding red pigment-concentrating hormone in black tiger shrimp penaeus monodon: implication of its function in molt and osmoregulation. | red pigment-concentrating hormone (rpch) is a member of the akh/rpch peptide family present mainly in crustaceans and insects. insect akh is responsible for metabolic functions whereas rpch plays a major role in the aggregation of red chromatophores in crustaceans. in this study, a full-length cdna of rpch of the black tiger shrimp, penaeus monodon (pmrpch) was cloned by rapid amplification of cdna ends strategies from the eyestalk rna. a 770 bp full-length pmrpch cdna harbored 279 bp of an open ... | 2014 | 24937259 |
| identification and characterization of a qm protein as a possible peptidoglycan recognition protein (pgrp) from the giant tiger shrimp penaeus monodon. | in an attempt to identify a peptidoglycan recognition protein (pgrp) in penaeus (penaeus) monodon, in vitro pull-down binding assays were used between shrimp proteins and purified peptidoglycan (pg). by gel electrophoresis and mass spectrometry followed by mascot program analysis, proteins from shrimp hemocyte peripheral membrane proteins showed significant homology to records for a qm protein, actin and prophenoloxidase 2 precursor (propo2), while proteins from cell-free plasma showed significa ... | 2014 | 24736204 |
| histopathological survey of potential biomarkers for the assessment of contaminant related biological effects in species of fish and shellfish collected from kuwait bay, arabian gulf. | the marine environment in kuwait is dominated by kuwait bay, a shallow, depositional habitat vital for the breeding and propagation of marine organisms. the bay receives effluent inputs from industrial centres, ports, sewage outflows along with discharges from power and desalination plants. the major classes of pollutant discharged into the bay include petroleum hydrocarbons, metals, nutrients, cooling water and hyper-saline water. further, the bay has been historically impacted by a deliberate ... | 2014 | 24680107 |
| characterization of intestinal bacteria in wild and domesticated adult black tiger shrimp (penaeus monodon). | the black tiger shrimp (penaeus monodon) is a marine crustacean of economic importance in the world market. to ensure sustainability of the shrimp industry, production capacity and disease outbreak prevention must be improved. understanding healthy microbial balance inside the shrimp intestine can provide an initial step toward better farming practice and probiotic applications. in this study, we employed a barcode pyrosequencing analysis of v3-4 regions of 16s rrna genes to examine intestinal b ... | 2014 | 24618668 |
| measurement of intracellular nitric oxide (no) production in shrimp haemocytes by flow cytometry. | a flow cytometric method to measure the production of intracellular nitric oxide (no) was adapted for use with shrimp haemocytes. we applied fluorescent probe 4-amino-5-methylamino-2',7'-difluorofluorescein diacetate (daf-fm da) for no detection in haemocytes from the tiger shrimp penaeus monodon, and used flow cytometry to quantify fluorescence intensity in individual haemocyte. the optimized protocol for intracellular no analysis consists to incubate haemocytes with daf-fm da at 10 μm for 60 m ... | 2013 | 24513495 |
| the development of a high density linkage map for black tiger shrimp (penaeus monodon) based on csnps. | transcriptome sequencing using illumina rna-seq was performed on populations of black tiger shrimp from india. samples were collected from (i) four landing centres around the east coastline (ec) of india, (ii) survivors of a severe wssv infection during pond culture (sur) and (iii) the andaman islands (ai) in the bay of bengal. equal quantities of purified total rna from homogenates of hepatopancreas, muscle, nervous tissue, intestinal tract, heart, gonad, gills, pleopod and lymphoid organs were ... | 2014 | 24465553 |
| identification and expression analysis of differentially expressed genes from shrimp (penaeus monodon) in response to low salinity stress. | four suppression subtractive hybridization (ssh) cdna libraries were constructed to identify differentially expressed salinity stress responsive genes of black tiger shrimp, penaeus monodon exposed to low (3 ppt) salinity conditions. forward and reverse ssh cdna libraries were developed from the gill and gut tissues of shrimp and clones having inserts larger than 300 bp were unidirectionally sequenced. based on the sequence homology search, the identified genes were categorized for their putativ ... | 2013 | 24436977 |
| effect of heat processing on antibody reactivity to allergen variants and fragments of black tiger prawn: a comprehensive allergenomic approach. | prawn allergy is one of the leading causes of ige-mediated hypersensitivity to food. alterations of ige-antibody reactivity to prawn allergens due to thermal processing are not fully understood. the aim of this study was to analyze the impact of heating on prawn allergens using a comprehensive allergenomic approach. | 2014 | 24420734 |
| haemocyte apoptosis of the tiger shrimp penaeus monodon exposed to cadmium. | this study investigated the effect of ambient cadmium (cd) on haemocyte apoptosis of the shrimp, penaeus monodon. cellular response was determined in cd-exposed (0, 0.05, 0.5 and 5 mg l(-1)) shrimp. results showed that 0.05 mg l(-1) cd(2+) had no significant effect on the haemocyte parameters during the 48 h exposure. cadmium at doses of 0.5 and 5 mg l(-1) depressed the total haemocyte count (thc), and increased reactive oxygen species (ros) production and apoptosis ratio in haemocytes. esterase ... | 2014 | 24374743 |
| [identification of 70 kda shrimp protein as a possible new allergen for shrimp allergy]. | a large-scale study of causative allergen components of shrimp allergies has never been conducted in japan. | 2013 | 24335423 |
| potential in vitro antimicrobial efficacy of holigarna arnottiana (hook f). | to explore the in vitro antimicrobial potential of holigarna arnottiana (h. arnottiana) against human and shrimp pathogenic bacteria and use gc-ms analysis to elucidate its antimicrobial principles. | 2014 | 24144126 |
| attenuation coefficients and absorbed gamma radiation energy of different varieties of potato, mango and prawn at different storage time and physiological condition. | attenuation coefficients of different varieties of gamma irradiated potato (kufri chandramukhi, kufri jyoti, and kufri sindhuri), mango (himsagar, langra, dashehri and fazli) and prawn (tiger prawn and fresh water prawn) of different storage time and physiological stages were determined. after six months storage attenuation coefficient of kufri chandramukhi was decreased by 30.8% with decrease of density and moisture content. decreasing trend of attenuation coefficient during storage was more pr ... | 2014 | 24128533 |
| tissue distribution of hepatopancreatic parvo-like virus of shrimp in freshwater rice-field crab, paratelphusa hydrodomous (herbst). | an attempt was made to determine the replication efficiency of hepatopancreatic parvo-like virus (hpv) of shrimp in different organs of freshwater rice-field crab paratelphusa hydrodomous (herbst) using bioassay, pcr, rt-pcr, elisa, western blot and q-pcr analyses. another attempt was made to use this crab as an alternative to penaeid shrimp for the large-scale production of hpv. this crab was found to be highly susceptible to hpv by intramuscular injection. the systemic hpv infection was confir ... | 2014 | 24117535 |
| characterization, expression and localization of valosin-containing protein in ovaries of the giant tiger shrimp penaeus monodon. | valosin-containing protein (vcp), a member of the atpase-associated with diverse cellular activity (aaa) family, was identified from the giant tiger shrimp (penaeus monodon). the full-length cdna of the pmvcp mrna consisted of 2,724 bp containing an orf of 2,367 bp corresponding to a deduced polypeptide of 788 amino acids. the deduced pmvcp protein contained two putative cdc48 domains (positions 17-103, e-value=2.00e-36 and 120-186, e-value=3.60e-11) and two putative aaa domains (positions 232-3 ... | 2014 | 24095778 |
| laem-singh virus: a probable etiological agent associated with monodon slow growth syndrome in farmed black tiger shrimp (penaeus monodon). | among the emerging diseases in shrimp aquaculture, monodon slow growth syndrome (msgs) is a major concern in south and southeast asia. shrimp farming in thailand was severely affected during 2000-2002 due to msgs, which caused an economic loss, of about us$ 300 million. msgs is characterized by abnormally slow growth with coefficients of size variation of >35 %, that has impacted p. monodon production in thailand. a new shrimp virus, laem-singh virus (lsnv) was identified to be associated in msg ... | 2012 | 23997445 |
| an evolutionarily ancient no synthase (nos) in shrimp. | nitric oxide (no) is a well known essential molecule that is involved in multiple functions such as neuron transduction, cardiac disease, immune responses, etc.; nitric oxide synthase (nos) is a critical enzyme that catalyzes the synthesis of it. a very few crustacean nos molecules were biochemically characterized so far. in the present study, we cloned and characterized a nos cdna from haemocytes of tiger shrimp (penaeus monodon) (pmnos). the full-length of pmnos cdna contained 3997 bp, includi ... | 2013 | 23994281 |
| characterization and expression of cell division cycle 2 (cdc2) mrna and protein during ovarian development of the giant tiger shrimp penaeus monodon. | the meiotic maturation of oocytes is regulated by the maturation-promoting factor (mpf), a complex of cdc2 (cdk1) and cyclin b. here, the complete open reading frame (orf) of cdc2 in penaeus monodon was characterized. pmcdc2 were 900bp in length corresponding to a polypeptide of 299 amino acids with the conserved thr14, tyr15 and thr161 residues. quantitative real-time pcr indicated that the expression level of pmcdc2 in wild intact broodstock was significantly increased in stages ii (vitellogen ... | 2013 | 23899716 |
| the microsporidian enterocytozoon hepatopenaei is not the cause of white feces syndrome in whiteleg shrimp penaeus (litopenaeus) vannamei. | the microsporidian enterocytozoon hepatopenaei was first described from thailand in 2009 in farmed, indigenous giant tiger shrimp penaeus (penaeus) monodon. the natural reservoir for the parasite is still unknown. more recently, a microsporidian closely resembling it in morphology and tissue preference was found in thai-farmed, exotic, whiteleg shrimp penaeus (litopenaeus) vannamei exhibiting white feces syndrome (wfs). our objective was to compare the newly found pathogen with e. hepatopenaei a ... | 2013 | 23856195 |
| ige reactivity of blue swimmer crab (portunus pelagicus) tropomyosin, por p 1, and other allergens; cross-reactivity with black tiger prawn and effects of heating. | shellfish allergy is a major cause of food-induced anaphylaxis, but the allergens are not well characterized. this study examined the effects of heating on blue swimmer crab (portunus pelagicus) allergens in comparison with those of black tiger prawn (penaeus monodon) by testing reactivity with shellfish-allergic subjects' serum ige. cooked extracts of both species showed markedly increased ige reactivity by elisa and immunoblotting, and clinical relevance of ige reactivity was confirmed by baso ... | 2013 | 23840718 |
| cloning of skeletal myosin heavy chain gene family from adult pleopod muscle and whole larvae of shrimps. | the physiological and biological properties of skeletal muscle in crustacea have not been well understood compared with those of vertebrates. the present study focused on myosin, the major protein in skeletal muscle, from shrimps. in our previous study, two full-length genes encoding myosin heavy chain (mhc), a large subunit of the myosin molecule, were cloned from abdominal fast skeletal muscle of kuruma marsupenaeus japonicus, black tiger penaeus monodon and pacific white penaeus vannamei shri ... | 2013 | 23526764 |
| characterization and expression analysis of the broad-complex (br-c) gene of the giant tiger shrimp penaeus monodon. | broad-complex (br-c) is the early ecdysone responsive gene encoding a family of zinc-finger transcription factors. in this study, the full-length cdna of the br-c gene of the giant tiger shrimp (penaeus monodon) was identified. pmbr-c was 1897 bp in length containing an orf of 1329 bp deducing to a polypeptide of 442 amino acids. pmbr-c was more abundantly expressed in ovaries than testes of p. monodon broodstock. the expression levels of pmbr-c mrna in ovaries of juveniles was significantly gre ... | 2013 | 23485783 |
| loop-mediated isothermal amplification combined with colorimetric nanogold for detection of the microsporidian enterocytozoon hepatopenaei in penaeid shrimp. | enterocytozoon hepatopenaei is an emerging microsporidian parasite that has been linked to recent losses caused by white faeces syndrome (wfs) in cultivated giant or black tiger shrimp penaeus (penaeus) monodon and whiteleg shrimp penaeus (litopenaeus) vannamei in asia. to more accurately assess its impact on shrimp production and to determine reservoir carriers for control measures, our objective was to establish a loop-mediated isothermal amplification (lamp) assay combined with colorimetric n ... | 2013 | 23387348 |
| comparative seasonal sterol profiles in edible parts of mediterranean fish and shellfish species. | the effect of different seasons on sterol content of seafoods was investigated. there were four sterols (cholesterol, sitosterol, desmosterol and stigmasterol) identified, with cholesterol being the predominant sterol. stigmasterol was a minor component in fish muscle, whilst sitosterol was one of the main phytosterols found in fish muscle. cholesterol content of fish consisted of 38-100% of total sterols in fish and 54-80% of total sterols in shellfish. the highest cholesterol content of fish m ... | 2013 | 23216341 |
| flow cytometric analysis of in vitro cytotoxicity of cadmium in haemocytes from the tiger shrimp, penaeus monodon. | this study investigated the toxic effects of cadmium on viability, reactive oxygen species (ros) production and non-specific esterase activity of penaeus monodon haemocytes in vitro, using a flow cytometric assay. after 6 h in vitro exposure with 10(-9)-10(-3) m cd(2+), cell viability, ros production and esterase activity of haemocytes from p. monodon were determined. results showed that at the lowest exposures (10(-9)-10(-6 )m), cd(2+) induced no effect on cell viability, ros production and est ... | 2013 | 23135305 |
| molecular cloning and mrna expression of m-phase phosphoprotein 6 gene in black tiger shrimp (penaeus monodon). | it is widely accepted that protein phosphorylation is a major control event in regulating cell cycle. in the present study, a novel m-phase phosphoprotein 6 (mpp6) was identified from black tiger shrimp penaeus monodon (designated as pmmpp6) by cdna library and race approaches. the full-length cdna of pmmpp6 was of 690 bp, including a 5'-terminal un-translated region (5'utr) of 68 bp, a 3'utr of 172 bp with a poly (a) tail, and an open reading frame (orf) of 450 bp encoding a polypeptide of 149 ... | 2013 | 23065290 |
| changes in oviduct structure in the black tiger shrimp, penaeus monodon, during ovarian maturation. | to examine the structure of the oviduct of the shrimp penaeus monodon. | 2012 | 23024052 |
| genetic analysis of black tiger shrimp (penaeus monodon) across its natural distribution range reveals more recent colonization of fiji and other south pacific islands. | the black tiger shrimp (penaeus monodon) has a natural distribution range from east africa to the south pacific islands. although previous studies of indo-pacific p. monodon have found populations from the indian ocean and australasia to differ genetically, their relatedness to south pacific shrimp remains unknown. to address this, polymorphisms at eight shared microsatellite loci and haplotypes in a 418-bp mtdna-cr (control region) sequence were examined across 682 p. monodon from locations spr ... | 2012 | 22957205 |
| cloning, expression, and localization of two types of fast skeletal myosin heavy chain genes from black tiger and pacific white shrimps. | the physiology and biochemistry of skeletal muscles in shrimps have been poorly understood compared with those from vertebrates. the present study was conducted focusing on myosin, the major protein in skeletal muscle, from adult specimens of black tiger penaeus monodon and pacific white penaeus vannamei shrimps. two genes encoding myosin heavy chain (mhc), a large subunit of the myosin molecule, were cloned from abdominal fast skeletal muscle and defined as mhca and mhcb according to our previo ... | 2012 | 22952142 |
| farm level and geographic predictors of antibiotic use in sri lankan shrimp farms. | black tiger shrimp penaeus monodon farming is important for sri lanka's rural development plans. consumer confidence is critical for the development and maintenance of export and domestic shrimp markets. public concern about the use of antimicrobial drugs and chemicals on shrimp farms, however, could threaten market access. we sought to identify high-risk areas and farm-level risk factors for antimicrobial use to inform the core messages and strategic placement of extension programs to help farm ... | 2012 | 22779210 |
| in vitro toxicity of nitrite on haemocytes of the tiger shrimp, penaeus monodon, using flow cytometric analysis. | this study investigated the in vitro effects of nitrite on reactive oxygen species (ros) production, no production, esterase activity and cell apoptosis of penaeus monodon haemocytes. haemocytes were in vitro exposed to different dose of nitrite (0, 0.1, 0.5, 1, 5 and 10 μm). cellular responses of nitrite-treated haemocytes were determined by flow cytometry. the results revealed that haemocytes treated by nitrite in vitro showed conspicuous time- and dose-dependent decreases in ros and no produc ... | 2012 | 22560987 |
| pathology associated with white spot virus (wsv) infection in wild broodstock of tiger prawns (penaeus monodon). | a total of six wild broodstocks of tiger prawns, penaeus monodon, were found positive for white spot virus (wsv) with an iq2000 detection kit. using histopathology, the intranuclear inclusion of haemocyte due to wsv infection was observed in the epithelium cells of the antennal gland, stomach and gills. this result confirmed that the wild broodstocks were positive with wsv without showing any white spot. additionally, histopathological examination also revealed an accumulation of haemocytes arou ... | 2012 | 24575228 |
| molecular cloning and tissue distribution of the toll receptor in the black tiger shrimp, penaeus monodon. | the black tiger shrimp (penaeus monodon) is economically important in many parts of the world, including thailand. shrimp immunity is similar to that of other invertebrate organisms; it consists of an innate immunity system. toll or toll-like receptors (tlrs) play an essential role in recognizing the cleaved form of the cytokine spätzle, which is processed by a series of proteolytic cascades activated by secreted recognition molecules. we isolated a full-length toll receptor from p. monodon. the ... | 2012 | 22535384 |
| identification of reproduction-related proteins and characterization of the protein disulfide isomerase a6 cdna in ovaries of the giant tiger shrimp penaeus monodon. | proteomic analysis was carried out for identification of proteins functionally involved in ovarian development of the giant tiger shrimp (penaeus monodon). a total of 335 protein spots including 183 spots from vitellogenic (stage ii) and 152 spots from mature (stage iv) ovaries of intact p. monodon broodstock were examined. of these, 75 (40.98%) and 59 (38.82%) spots significantly matched known proteins in the databases, respectively. in addition, 270 protein spots including 167 and 103 spots fr ... | 2012 | 22406237 |
| identification of reproduction-related proteins and characterization of proteasome alpha 3 and proteasome beta 6 cdnas in testes of the giant tiger shrimp penaeus monodon. | cellular proteomic analysis was carried out to identify reproduction-related proteins in testes of wild and domesticated broodstock of penaeus monodon. in total, 642 protein spots were characterized and 287 spots (44.70%) significantly matched protein sequences in the databases (p<0.05). to examine a role of the proteasome system in testicular development of p. monodon, the expression profiles of proteasome alpha 3 subunit (pmpsma3) and proteasome beta 6 (pmpsmb6) mrna in different groups of dom ... | 2012 | 22361323 |
| microsatellite dna markers: evaluating their potential for estimating the proportion of hatchery-reared offspring in a stock enhancement programme. | we describe a statistical method for estimating the effectiveness of a stock enhancement programme using nuclear dna loci. it is based on knowing the population allele frequencies and the genotypes of the hatchery parents (mother only, or mother and father), and on determining the probability that a wild-born animal will by chance have a genotype consistent with hatchery origin. we show how to estimate the proportion of released animals in the wild population, and its standard error. the method ... | 2004 | 15078464 |
| photostimulated luminescence detection of irradiated shellfish: international interlaboratory trial. | an interlaboratory trial was conducted to validate photostimulated luminescence (psl) detection of irradiated shellfish. five species of shellfish (nephrops norvegicus, mussels, black tiger prawns, brown shrimps, and king scallops) were presented blind as nonirradiated and irradiated to 0.5 and 2.5 kgy. precharacterization analysis of each product and treatment was performed on both whole (including shell) and intestinal samples. the results for whole samples (including shell) confirmed that the ... | 2008 | 14632401 |
| thermoluminescence detection of irradiated shellfish: international interlaboratory trial. | an international interlaboratory trial was conducted using thermoluminescence for the detection of irradiated shellfish, aimed at validating the method for routine use. nephrops norvegicus, mussels, brown shrimps, black tiger prawns, and king scallops were presented as nonirradiated and irradiated to 0.5 and 2.5 kgy. the protocol called for the use of 3 preparation methods: extraction of silicates from whole shellfish by acid hydrolysis and physical separation, and of carbonates from powdered sh ... | 2008 | 14632400 |
| high prevalence of enterocytozoon hepatopenaei in shrimps penaeus monodon and litopenaeus vannamei sampled from slow growth ponds in india. | hepatopancreatic microsporidiosis in cultivated litopenaeus vannamei and penaeus monodon is caused by the newly emerged pathogen enterocytozoon hepatopenaei (ehp). it has been detected in shrimp cultured in china, vietnam and thailand and is suspected to have occurred in malaysia and indonesia and to be associated with severely retarded growth. due to retarded shrimp growth being reported at farms in the major grow-out states of tamilnadu, andhra pradesh and odisha in india, shrimp were sampled ... | 2016 | 27503918 |
| identification of a clip domain serine proteinase involved in immune defense in chinese mitten crab eriocheir sinensis. | clip-domain serine proteinase is an important serine proteinase family involved in many biological processes, which is only found in invertebrates. in the present study, the full-length cdna of a clip domain serine proteinase (designed as escdsp) gene was cloned from chinese mitten crab eriocheir sinensis using rapid amplification of cdna ends (race) technique. it was of 1488 bp with an open reading frame (orf) of 1134 bp encoding a polypeptide of 377 amino acids. there were a signal peptide, a ... | 2018 | 29305333 |
| the cyclin-dependent kinase 2 (cdk2) mediates hematopoiesis through g1-to-s transition in chinese mitten crab eriocheir sinensis. | cyclin-dependent kinases (cdks), a family of cell cycle-related serine/threonine kinases, participate in various biological processes, and play crucial roles in the innate immunity. in the present study, a cdk2 (designed as escdk2) with a serine/threonine protein kinase catalytic domain was identified from chinese mitten crab (eriocheir sinensis). the full-length cdna sequence of escdk2 was of 2405 bp with an open reading frame (orf) of 909 bp. escdk2 shared 66%-81% sequence similarities with pr ... | 2018 | 29198777 |
| a cytosolic sensor, pmddx41, mediates antiviral immune response in black tiger shrimp penaeus monodon. | dead (asp-glu-ala-asp)-box polypeptide 41 (ddx41), a receptor belonging to the dexd family, has recently been identified as an intracellular dna sensor in vertebrates. here, we report on the identification and functional characterization of pmddx41, the first cytosolic dna sensor in shrimp. by searching a penaeus monodon expressed sequence tag (est) database (http://pmonodon.biotec.or.th), three cdna fragments exhibiting similarity to ddx41 in various species were identified and assembled, resul ... | 2018 | 29248385 |
| characterization and expression analysis of a chitinase gene (pmchi-5) from black tiger shrimp (penaeus monodon) under pathogens infection and ambient ammonia-n stress. | chitinases are crucial enzymes for crustaceans. previous researches had already revealed that chitinases play important roles in digestion, molting and defense against viruses. in the present study, a chitinase cdna was identified from black tiger shrimp (penaeus monodon) and designated as pmchi-5. the full-length pmchi-5 cdna was 2860 bp in size, containing an open reading frame (orf) of 1731 bp that encoded a protein of 576 amino acids with a deduced molecular weight of 64.8 kda. expression of ... | 2018 | 29100985 |
| a multiplex bead-based assay for immune gene expression analysis in shrimp. | here, we developed a 9-plex bead-based array as a tool to evaluate molecular effects on transcription levels of immune-related genes in the black tiger shrimp (penaeus monodon). the bead array technology allows simultaneous detection of multiple target genes in a single sample, reducing time, labor and cost. the oligonucleotide probes were designed to target eight immune-related genes that involve in antimicrobial activity, melanization, pathogen pattern recognition proteins, lysozyme and one ho ... | 2017 | 28923715 |
| qualitative proteomic analysis of tipula oleracea nudivirus occlusion bodies. | nudiviruses are arthropod-specific large double-stranded circular dna viruses, related to baculoviruses, which replicate in the nucleus of the cells they infect. to date, six fully sequenced nudiviral genomes are available in databases, and the protein profile from nudivirus particles was mainly characterized by page. however, only a few direct matches have been completed between genomic and proteomic data, with the exception of the major occlusion body protein from penaeus monodon nudivirus and ... | 2017 | 28284235 |
| qualitative proteomic analysis of tipula oleracea nudivirus (tonv) occlusion bodies. | nudiviruses are arthropod-specific large double-stranded circular dna viruses, related to baculoviruses, which replicate in the nucleus of the cells they infect. up to date six fully sequenced nudiviral genomes are available in databases and protein profile from nudivirus particles were mainly characterized by polyacrylamide gel electrophoresis. however, only few direct matches were completed between genomic and proteomic data to the exception of the major occlusion body protein from penaeus mon ... | 2016 | 27902361 |