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carbazole hydroxylation by the filamentous fungi of the cunninghamella species.nitrogen heterocyclic compounds, especially carbazole, quinolone, and pyridine are common types of environmental pollutants. carbazole has a toxic influence on living organisms, and the knowledge of its persistence and bioconversion in ecosystems is still not complete. there is an increasing interest in detoxification of hazardous xenobiotics by microorganisms. in this study, the ability of three filamentous fungi of the cunninghamella species to eliminate carbazole was evaluated. the cunningham ...201526276273
study of model systems to test the potential function of artemia group 1 late embryogenesis abundant (lea) proteins.embryos of the brine shrimp, artemia franciscana, are genetically programmed to develop either ovoviparously or oviparously depending on environmental conditions. shortly upon their release from the female, oviparous embryos enter diapause during which time they undergo major metabolic rate depression while simultaneously synthesize proteins that permit them to tolerate a wide range of stressful environmental events including prolonged periods of desiccation, freezing, and anoxia. among the know ...201626462928
feeding truncated heat shock protein 70s protect artemia franciscana against virulent vibrio campbellii challenge.the 70 kda heat shock proteins (hsp70s) are highly conserved in evolution, leading to striking similarities in structure and composition between eukaryotic hsp70s and their homologs in prokaryotes. the eukaryotic hsp70 like the dnak (escherichia coli equivalent hsp70) protein, consist of three functionally distinct domains: an n-terminal 44-kda atpase portion, an 18-kda peptide-binding domain and a c-terminal 10-kda fragment. previously, the amino acid sequence of eukaryotic (the brine shrimp ar ...201323092733
alachlor oxidation by the filamentous fungus paecilomyces marquandii.alachlor, a popular chloroacetanilide herbicide, can be a potential health risk factor. soil microorganisms are primarily responsible for conversion and migration of alachlor in natural environment, but knowledge concerning alachlor biodegradation is not complete. therefore, we studied the ability of paecilomyces marquandii, soil fungus tolerant to heavy metals, to eliminate alachlor and proposed a new pathway of its transformation. after 7 days of incubation only 3.3% of alachlor was detected f ...201323974531
the effects of feeding with synbiotic (pediococcus acidilactici and fructooligosaccharide) enriched adult artemia on skin mucus immune responses, stress resistance, intestinal microbiota and performance of angelfish (pterophyllum scalare).the aim of this study was to evaluate the effects of feeding on synbiotic (pediococcus acidilactici and fructooligosaccharide) enriched adult artemia franciscana on skin mucus immune responses, stress resistance, intestinal microbiota and growth performance of angelfish (pterophyllum scalare). three hundred and sixty fish with initial weight 3.2 ± 0.13 g were randomly divided into twelve aquaria (50 l) assigned to four groups in triplicates. fish were fed for 7 weeks with dietary treatments, inc ...201627150050
artificial and factitious foods support the development and reproduction of the predatory mite amblyseius swirskii.the generalist predatory mite amblyseius swirskii athias-henriot (acari: phytoseiidae) was reared on ephestia kuehniella zeller eggs (lepidoptera: pyralidae), decapsulated dry cysts of the brine shrimp artemia franciscana kellogg (anostraca: artemiidae), and on meridic artificial diets (composed of honey, sucrose, tryptone, yeast extract, and egg yolk) supplemented with pupal hemolymph of the chinese oak silkworm antheraea pernyi (guérin-méneville) (lepidoptera: saturniidae) (ad1), with e. kuehn ...201424154947
the suppressor of aac2 lethality sal1 modulates sensitivity of heterologously expressed artemia adp/atp carrier to bongkrekate in yeast.the adp/atp carrier protein (aac) expressed in artemia franciscana is refractory to bongkrekate. we generated two strains of saccharomyces cerevisiae where aac1 and aac3 were inactivated and the aac2 isoform was replaced with artemia aac containing a hemagglutinin tag (araac-ha). in one of the strains the suppressor of δaac2 lethality, sal1, was also inactivated but a plasmid coding for yeast aac2 was included, because the araacδsal1δ strain was lethal. in both strains araac-ha was expressed and ...201324073201
surface area estimation of the gut segments of artemia franciscana nauplii fed with mnn9 yeast vs. wild type yeast. 201325141655
degradation and toxicity reduction of the endocrine disruptors nonylphenol, 4-tert-octylphenol and 4-cumylphenol by the non-ligninolytic fungus umbelopsis isabellina.nonylphenol (np), 4-tert-octylphenol (4-t-op) and 4-cumylphenol (4-cp) are pollutants that are known as endocrine disruptors mainly due to their estrogen-mimicking activity. these phenolic substances are used in a wide range of industrial and commercial applications. in the present study, biodegradation of tnp, 4-t-op and 4-cp using the non-ligninolytic fungus umbelopsis isabellina was investigated. after 12h of incubation, more than 90% of initially applied tnp, 4-t-op and 4-cp (25mgl(-1)) were ...201626492175
probing the protective mechanism of poly-ß-hydroxybutyrate against vibriosis by using gnotobiotic artemia franciscana and vibrio campbellii as host-pathogen model.the compound poly-ß-hydroxybutyrate (phb), a polymer of the short chain fatty acid ß-hydroxybutyrate, was shown to protect experimental animals against a variety of bacterial diseases, (including vibriosis in farmed aquatic animals), albeit through undefined mechanisms. here we aimed at unraveling the underlying mechanism behind the protective effect of phb against bacterial disease using gnotobiotically-cultured brine shrimp artemia franciscana and pathogenic vibrio campbellii as host-pathogen ...201525822312
enhanced resistance against vibrio harveyi infection by carvacrol and its association with the induction of heat shock protein 72 in gnotobiotic artemia franciscana.induction of hsp72 is a natural response of stressed organisms that protects against many insults including bacterial diseases in farm (aquatic) animals. it would therefore be of great health benefit to search for natural compounds that are clinically safe yet able to induce hsp72 in animals. the phenolic compound carvacrol, an approved food component, had been shown in in vitro study to act as a co-inducer of hsp72, enhancing hsp72 production only in combination with a bona fide stress compared ...201728303510
the gnotobiotic brine shrimp (artemia franciscana) model system reveals that the phenolic compound pyrogallol protects against infection through its prooxidant activity.the phenolic compound pyrogallol is the functional unit of many polyphenols and currently there has been a growing interest in using this compound in human and animal health owing to its health-promoting effects. the biological actions of pyrogallol moiety (and polyphenols) in inducing health benefitting effects have been studied; however, the mechanisms of action remain unclear yet. here, we aimed at unravelling the underlying mechanism of action behind the protective effects of pyrogallol agai ...201526459033
the apparent quorum-sensing inhibitory activity of pyrogallol is a side effect of peroxide production.there currently is more and more interest in the use of natural products, such as tea polyphenols, as therapeutic agents. the polyphenol compound pyrogallol has been reported before to inhibit quorum-sensing-regulated bioluminescence in vibrio harveyi. here, we report that the addition of 10 mg · liter(-1) pyrogallol protects both brine shrimp (artemia franciscana) and giant river prawn (macrobrachium rosenbergii) larvae from pathogenic vibrio harveyi, whereas the compound showed relatively low ...201323545532
monitoring of vibrio harveyi quorum sensing activity in real time during infection of brine shrimp larvae.quorum sensing, bacterial cell-to-cell communication, has been linked to the virulence of pathogenic bacteria. indeed, in vitro experiments have shown that many bacterial pathogens regulate the expression of virulence genes by this cell-to-cell communication process. moreover, signal molecules have been detected in samples retrieved from infected hosts and quorum sensing disruption has been reported to result in reduced virulence in different host-pathogen systems. however, data on in vivo quoru ...201222673627
effect of aloe barbadensis miller juice on oxidative stress biomarkers in aerobic cells using artemia franciscana as a model.this study reports on the induction of oxidative stress in aerobic cell systems by aloe barbadensis miller (aloe vera) juice using the salt water crustacean artemia franciscana as a model. a consistent pattern was observed in which artemia franciscana nauplii responded to aloe vera juice exposure with a decrease in the overall activity of redox related enzymes. exposure of artemia franciscana to sub-lethal levels of aloe vera juice resulted in a decreased activity of thioredoxin reductase, gluta ...201019610042
moisture source and diet affect development and reproduction of orius thripoborus and orius naivashae, two predatory anthocorids from southern africa.the effect of moisture source and diet on the development and reproduction of the pirate bugs, orius thripoborus (hesse) and orius naivashae (poppius) (hemiptera: anthocoridae) was examined in the laboratory. both species had been collected in and around sugarcane fields in south africa. supplementing eggs of the flour moth ephestia kuehniella (zeller) (lepidoptera: pyralidae) with a green bean pod as a moisture source yielded better nymphal survival and faster development, as compared with free ...201222935002
multigenerational demographic responses of sexual and asexual artemia to chronic genotoxicity by a reference mutagen.genotoxins are capable of multigenerational impacts on natural populations via dna damage and mutations. sexual reproduction is assumed to reduce the long term consequences of genotoxicity for individual fitness and should therefore reduce population level effects. however, rather few empirical studies have quantified the magnitude of this effect. we tried to analyse the multigenerational demographic responses of sexual artemia franciscana and asexual artemia parthenogenetica due to chronic geno ...201324141038
may arsenic pollution contribute to limiting artemia franciscana invasion in southern spain?limited information exists regarding the complex interactions between biological invasions, pollution, and climate change. most studies indicate that pollution tends to favor invasive species. here, we provide evidence that arsenic (as) pollution may have a role in limiting the invasion of the exotic brine shrimp artemia franciscana. we tested as toxicity in natural populations of artemia parthenogenetica (a native taxon) and a. franciscana from localities in southern spain with differing degree ...201626925327
effects of genotoxicity and its consequences at the population level in sexual and asexual artemia assessed by analysis of inter-simple sequence repeats (issr).there is considerable evidence that genetic damage in organisms occurs in the environment as a result of exposure to genotoxins and ionising radiation, but we have limited understanding of the extent to which this results in adverse consequences at a population level. we used inter-simple sequence repeat (issr) markers to quantify genotoxic effects of the mutagen ethylmethane sulfonate (ems) on a sexual (artemia franciscana) and an asexual (artemia parthenogenetica) species of brine shrimp. the ...201323872504
cryptic microsporidian parasites differentially affect invasive and native artemia spp.we investigated the host specificity of two cryptic microsporidian species (anostracospora rigaudi and enterocytospora artemiae) infecting invasive (artemia franciscana) and native (artemia parthenogenetica) hosts in sympatry. anostracospora rigaudi was on average four times more prevalent in the native host, whereas e. artemiae was three times more prevalent in the invasive host. infection with an. rigaudi strongly reduced female reproduction in both host species, whereas infection with e. arte ...201323851079
cytological, molecular and life cycle characterization of anostracospora rigaudi n. g., n. sp. and enterocytospora artemiae n. g., n. sp., two new microsporidian parasites infecting gut tissues of the brine shrimp artemia.two new microsporidia, anostracospora rigaudi n. g., n. sp., and enterocytospora artemiae n. g., n. sp. infecting the intestinal epithelium of artemia parthenogenetica bowen and sterling, 1978 and artemia franciscana kellogg, 1906 in southern france are described. molecular analyses revealed the two species belong to a clade of microsporidian parasites that preferentially infect the intestinal epithelium of insect and crustacean hosts. these parasites are morphologically distinguishable from oth ...201323731593
differential responses of sexual and asexual artemia to genotoxicity by a reference mutagen: is the comet assay a reliable predictor of population level responses?the impact of chronic genotoxicity to natural populations is always questioned due to their reproductive surplus. we used a comet assay to quantify primary dna damage after exposure to a reference mutagen ethyl methane sulfonate in two species of crustacean with different reproductive strategies (sexual artemia franciscana and asexual artemia parthenogenetica). we then assessed whether this predicted individual performance and population growth rate over three generations. artemia were exposed t ...201323414719
why join groups? lessons from parasite-manipulated artemia.grouping behaviours (e.g. schooling, shoaling and swarming) are commonly explicated through adaptive hypotheses such as protection against predation, access to mates or improved foraging. however, the hypothesis that aggregation can result from manipulation by parasites to increase their transmission has never been demonstrated. we investigated this hypothesis using natural populations of two crustacean hosts (artemia franciscana and artemia parthenogenetica) infected with one cestode and two mi ...201323351125
further characterization of the cathepsin l-associated protein and its gene in two species of the brine shrimp, artemia.the major cysteine protease in embryos and larvae of the brine shrimp artemia franciscana is a heterodimer composed of a cathepsin l-like polypeptide of 28.5 kda and a 31.5 kda polypeptide called the cathepsin l-associated protein or clap. in a previous study, clap was shown to be a cell adhesion protein containing two fas i domains and two gtp/atp binding sites known as walker a and b motifs. here, we have characterized clap and its genes to better understand the role of this protein in artemia ...200616962350
comparing the acute response to cadmium toxicity of nauplii from different populations of artemia.the lethal responses to cadmium of instar ii nauplii from eight populations of artemia belonging to the species artemia franciscana, artemia salina, artemia persimilis, and artemia parthenogenetica have been compared. generalized linear models were used to fit mortality. the model indicates that there is a relationship between species, type of population, and mortality rate. the two populations of a. franciscana were the most sensitive to cadmium toxicity (median lethal concentration, lc50, of 9 ...200211833813
high dna sequence variability at the alpha 1 na/k-atpase locus of artemia franciscana (brine shrimp): polymorphism in a gene for salt-resistance in a salt-resistant organism.we previously reported that the na/k-atpase alpha 1 subunit coding gene showed signs of being a very polymorphic locus in artemia franciscana. this species is adapted to highly saline waters, and the na/k-atpase alpha 1 isoform presumably plays a key role in this adaptation. therefore, we were interested in further study of the alpha 1 na/k-atpase polymorphisms to examine whether they might be due to an adaptation to salt resistance driven by natural selection. using coding sequences from 10 gen ...200010677846
identification and characterization of a masculinizer (masc) gene involved in sex differentiation in artemia.the sex of relatively primitive animals such as invertebrates is mostly determined by environmental factors and chromosome ploidy. heteromorphic chromosomes may also play an important role, as in the zw system in lepidopterans. however, the mechanisms of these various sex determination systems are still largely undefined. in the present study, a masculinizer gene (ar-masc) was identified in the crustacean artemia franciscana kellogg 1906. sequence analysis revealed that the 1140-bp full-length o ...201728300613
group 3 late embryogenesis abundant proteins from embryos of artemia franciscana: structural properties and protective abilities during desiccation.group 3 late embryogenesis abundant (lea) proteins are highly hydrophilic, and their expression is associated with desiccation tolerance in both plants and animals. here we show that two lea proteins from embryos of artemia franciscana, afrlea2 and afrlea3m, are intrinsically disordered in solution but upon desiccation gain secondary structure, as measured by circular dichroism. trifluoroethanol and sodium dodecyl sulfate are both shown to induce α-helical structure in afrlea2 and afrlea3m. bioi ...201425244376
treatment of vibriosis in european sea bass larvae, dicentrarchus labrax l., with oxolinic acid administered by bath or through medicated nauplii of artemia franciscana (kellogg): efficacy and residual kinetics.european sea bass larvae were challenged by bath with listonella anguillarum strain 332a, 2.5×10(7) cfuml(-1) for 1h. fish either received no treatment or oral treatment with artemia franciscana (kellog) nauplii enriched with oxolinic acid, or bath treatments with oxolinic acid. medication commenced 1day following challenge and was performed on days 1, 3 and 5 post-challenge at a dosage of 20mgl(-1) for 2h for bath treatments, while two doses each of 750 nauplii per fish were administered daily ...201222670590
[evaluation of psilocybe cubensis (agaricales, basidiomycota) toxicity over artemia franciscana (crustacea, anostraca)].psilocybe cubensis is a species with psychodysleptic action that grows on cattle dung in pastures in the tropics and subtropics. this fungus has been widely used in mexico since ancient times both for ceremonies and rituals, as well as for healing or medicinal purposes. artemia franciscana is a crustacean frequently used as a model organism for toxicity testing.201322728109
non-lethal heat shock induces hsp70 and hmgb1 protein production sequentially to protect artemia franciscana against vibrio campbellii. 201525463291
bacillus sp. lt3 improves the survival of gnotobiotic brine shrimp (artemia franciscana) larvae challenged with vibrio campbellii by enhancing the innate immune response and by decreasing the activity of shrimp-associated vibrios.bacteria belonging to the genus bacillus are amongst the most intensively studied group of bacteria for use as probiotics in aquaculture. however, the exact mechanism of action of these bacteria is often not well described, and the microbiota that are naturally present in cultures of test organisms often compromise the interpretation of the results. the present study aimed to evaluate the putative probiotic effect of bacillus sp. lt3 in a model system with gnotobiotic brine shrimp artemia franci ...201425190276
the vibrio campbellii quorum sensing signals have a different impact on virulence of the bacterium towards different crustacean hosts.pathogenic bacteria communicate with small signal molecules in a process called quorum sensing, and they often use different signal molecules to regulate virulence gene expression. vibrio campbellii, one of the major pathogens of aquatic organisms, regulates virulence gene expression by a three channel quorum sensing system. here we show that although they use a common signal transduction cascade, the signal molecules have a different impact on the virulence of the bacterium towards different ho ...201324055027
absence of ca2+-induced mitochondrial permeability transition but presence of bongkrekate-sensitive nucleotide exchange in c. crangon and p. serratus.mitochondria from the embryos of brine shrimp (artemia franciscana) do not undergo ca(2+)-induced permeability transition in the presence of a profound ca(2+) uptake capacity. furthermore, this crustacean is the only organism known to exhibit bongkrekate-insensitive mitochondrial adenine nucleotide exchange, prompting the conjecture that refractoriness to bongkrekate and absence of ca(2+)-induced permeability transition are somehow related phenomena. here we report that mitochondria isolated fro ...201222768139
evaluation of a battery of marine species-based bioassays against raw and treated municipal wastewaters.the present study evaluates a battery of marine species-based bioassays against chemically characterized municipal wastewater samples (raw and wwtp treated). we estimated dunaliella tertiolecta growth rate inhibition (24-96h ic50 values), artemia franciscana immobilization (24h lc50 values), mussel hemocytes viability and lipid peroxidation enhancement (in terms of neutral red retention assay/nrrt and malondialdehyde/mda content, respectively) in influent- and wwtp effluent-treated species. we f ...201727676080
an interlaboratory comparison of nanosilver characterisation and hazard identification: harmonising techniques for high quality data.within the fp7 eu project nanovalid a consortium of six partners jointly investigated the hazard of silver nanoparticles (agnps) paying special attention to methodical aspects that are important for providing high-quality ecotoxicity data. laboratories were supplied with the same original stock dispersion of agnps. all partners applied a harmonised procedure for storage and preparation of toxicity test suspensions. altogether ten different toxicity assays with a range of environmentally relevant ...201626638016
ecotoxicity of diethylene glycol and risk assessment for marine environment.diethylene glycol (deg) is a chemical compound used during offshore oil activities to prevent hydrate formation, and it may be released into the sea. a full ecotoxicological characterization is required according to european and italian regulations for chemical substances. we have evaluated long-term toxic effects of deg on indicator species of the marine environment as algae (phaeodactylum tricornutum), crustaceans (artemia franciscana), molluscs (tapes philippinarum) and fish (dicentrarchus la ...201525463226
improved tolerance to salt and water stress in drosophila melanogaster cells conferred by late embryogenesis abundant protein.mechanisms that govern anhydrobiosis involve the accumulation of highly hydrophilic macromolecules, such as late embryogenesis abundant (lea) proteins. group 1 lea proteins comprised of 181 (aflea1.1) and 197 (aflea1.3) amino acids were cloned from embryos of artemia franciscana and expressed in drosophila melanogaster cells (kc167). confocal microscopy revealed a construct composed of green fluorescent protein (gfp) and aflea1.3 accumulates in the mitochondria (aflea1.3-gfp), while aflea1.1-gfp ...201323376561
partial sequence of the shrimp penaeus notialis mitochondrial genome.about half of the mitochondrial dna of the shrimp penaeus notialis (crustacea: decapoda) has been cloned (in 2 overlapping fragments of 7.9 kb and 1 kb) and partially sequenced. the gene content and arrangement are identical to that of the homologous domain in drosophila yakuba. intergenic nucleotides are scarce and a 982 bp non-coding sequence exhibit features similar to that of mtdna control regions. the gene organization and the trna structures differentiate the penaeus notialis mitochondrial ...19968881281
supplemental food that supports both predator and pest: a risk for biological control?supplemental food sources to support natural enemies in crops are increasingly being tested and used. this is particularly interesting for generalist predators that can reproduce on these food sources. however, a potential risk for pest control could occur when herbivores also benefit from supplemental food sources. in order to optimize biological control, it may be important to select food sources that support predator populations more than herbivore populations. in this study we evaluated the ...201525349063
developmental and reproductive fitness of orius laevigatus (hemiptera: anthocoridae) reared on factitious and artificial diets.the developmental and reproductive fitness of the polyphagous predator orius laevigatus (fieber) (hemiptera: anthocoridae) was compared on two factitious foods and four artificial diets. adults fed factitious foods (ephestia kuehniella zeller eggs and artemia franciscana kellogg cysts) performed better than those fed artificial diets. among the artificial diets, a diet composed of liver and ground beef scored better than meridic diets based on egg yolk. within the egg yolk-based artificial diets ...200818767719
aph-1, a pou homeobox gene expressed in the salt gland of the crustacean artemia franciscana.we characterized the first pou-homeoprotein in a crustacean (designated aph-1 for artemia pou-homeoprotein, embl y15070). the amino acid sequence of the aph-1 pou-domain is identical, except for two residues, to that of the two class iii pou proteins cf1-a (drosophila) and pou-m1 (bombyx mori). southern blot analysis suggests that crustaceans have only one class iii pou gene. rt-pcr and whole-mount in situ hybridization show that aph-1 mrna is present in larvae specifically in the salt gland, an ...199910495289
effects of polystyrene microbeads in marine planktonic crustaceans.plastic debris accumulates in the marine environment, fragmenting into microplastics (mp), causing concern about their potential toxic effects when ingested by marine organisms. the aim of this study was to verify whether 0.1µm polystyrene beads are likely to trigger lethal and sub-lethal responses in marine planktonic crustaceans. mp build-up, mortality, swimming speed alteration and enzyme activity (cholinesterases, catalase) were investigated in the larval stages of amphibalanus amphitrite ba ...201728750293
a case study to optimise and validate the brine shrimp artemia franciscana immobilisation assay with silver nanoparticles: the role of harmonisation.brine shrimp artemia sp. has been recognised as an important ecotoxicity and nanotoxicity test model organism for salt-rich aquatic environments, but currently there is still no harmonised testing protocol which would ensure the comparable results for hazard identification. in this paper we aimed to design the harmonised protocol for nanomaterial toxicity testing using artemia franciscana and present a case study to validate the protocol with silver nanoparticles (agnps). we (i) revised the exis ...201626895539
artemia franciscana as a vector for infectious myonecrosis virus (imnv) to litopenaeus vannamei juvenile.in 2004, the infectious myonecrosis virus (imnv) was recognized as the main cause of litopenaeusvannamei shrimp culture's drop in brazil. in health animal control programs, in order to reduce virus prevalence in production units it is necessary to screen live feed used. among live diets used in aquaculture, the brine shrimp artemia sp. is essential in crustacean larviculture and maturation. the aim of the present study was to investigate the susceptibility of artemiafranciscana to imnv through a ...201525676109
detoxification and color removal of congo red by a novel dietzia sp. (dts26) - a microcosm approach.the present study deals with the decolorization and detoxification of congo red (cr) by a novel marine bacterium dietzia sp. (dts26) isolated from divar island, goa, india. the maximum decolorization of 94.5% (100 mg l(-1)) was observed under static condition within 30 h at ph 8 and temperature 32±2°c. bacterially treated samples could enhance the light intensity by 38% and the primary production levels 5 times higher than the untreated. the strain was also able to reduce cod by 86.4% within 30 ...201525600715
the distribution of fruit and seed toxicity during development for eleven neotropical trees and vines in central panama.secondary compounds in fruit mediate interactions with natural enemies and seed dispersers, influencing plant survival and species distributions. the functions of secondary metabolites in plant defenses have been well-studied in green tissues, but not in reproductive structures of plants. in this study, the distribution of toxicity within plants was quantified and its influence on seed survival was determined in central panama. to investigate patterns of allocation to chemical defenses and shift ...201323843965
selenium biotransformations in an engineered aquatic ecosystem for bioremediation of agricultural wastewater via brine shrimp production.an engineered aquatic ecosystem was specifically designed to bioremediate selenium (se), occurring as oxidized inorganic selenate from hypersalinized agricultural drainage water while producing brine shrimp enriched in organic se and omega-3 and omega-6 fatty acids for use in value added nutraceutical food supplements. selenate was successfully bioremediated by microalgal metabolism into organic se (seleno-amino acids) and partially removed via gaseous volatile se formation. furthermore, filter- ...201323621086
kinase inhibitory, haemolytic and cytotoxic activity of three deep-water sponges from north western australia and their fatty acid composition.the c-amp dependent protein kinase (pka) inhibition, haemolytic activity, and cytoxicity of 21 extracts obtained from north western australian sponges collected from depths of 84-135 m were investigated. hexane extracts from ircinia/sarcotragus sp. and geodia sp. displayed pka inhibitory activities of 100 and 97% respectively (at 100 microg/ml), while aq. methanol extracts from haliclona sp. exhibited potent haemolytic activity (75%) and hexane extracts from geodia sp. were highly toxic (88%) to ...201122312740
toxicity evaluation of diethylene glycol and its combined effects with produced waters of off-shore gas platforms in the adriatic sea (italy): bioassays with marine/estuarine species.diethylene glycol (deg) is commonly used to dehydrate natural gas in off-shore extraction plants and to prevent formation of gas hydrates. it may be released into the sea accidentally or in discharged produced waters (pws). pws samples from off-shore gas platforms in the adriatic sea (italy) have been used in this study. the objectives of the study were: a) to evaluate the toxicity of deg for marine organisms; b) to evaluate if a high deg content in pws may alter their toxicity; c) to verify whe ...201222245535
seawater ecotoxicity of monoethanolamine, diethanolamine and triethanolamine.monoethanolamine (mea), diethanolamine (dea) and triethanolamine (tea) are compounds with potential acute, sub-chronic and chronic toxicity effects towards aquatic species. a literature review highlighted the existence of a gap in the knowledge on their toxicity with saltwater testing species. a battery of toxicity tests including the alga phaeodactylum tricornutum bohlin, the bivalve molluscs crassostrea gigas (thunberg) and mytilus galloprovincialis (lmk), and the crustacean artemia franciscan ...201020022426
toxicity of untreated wood leachates towards two saltwater organisms (crassostrea gigas and artemia franciscana).wood is widely used in the development of freshwater, estuarine and marine coastlines. timbers last according to their content of naturally occurring preservatives (mostly phenols and aldehydes), produced to prevent decay from biotic agents. when untreated woods are exposed to aquatic media, leachates are generated with likely toxic effects on the target environment. the potential impact on saltwaters of leachates from some untreated timbers of both native and tropical species has been assessed. ...200717141956
exploring embryonic germ line development in the water flea, daphnia magna, by zinc-finger-containing vasa as a marker.vasa is an atp-dependent rna helicase belonging to the dead-box family that, in many organisms, is specifically expressed in germ line cells throughout the life cycle, making it a powerful molecular marker to study germ line development. to obtain further information on germ line development in crustaceans, we cloned vasa cdnas from three branchiopod species: water fleas daphnia magna and moina macrocopa, and brine shrimp artemia franciscana. rna helicase domains in branchiopod vasa were highly ...200515939379
larval helminths in the invasive american brine shrimp artemia franciscana throughout its annual cycle.one of the best examples of rapid displacement of native species by an invader is the eradication of native artemia salina and a. parthenogenetica in the mediterranean by the introduced american a. franciscana. previous studies based on sampling from limited time periods suggest that the success of the american species as a competitor may be due partly to different parasite burden, since native artemia spp. have high cestode infection rates regulating their density. the aim of this study is to t ...201425119350
the early bird gets the shrimp: confronting assumptions of isotopic equilibrium and homogeneity in a wild bird population.1. because stable isotope distributions in organic material vary systematically across energy gradients that exist in ecosystems, community and population structures, and in individual physiological systems, isotope values in animal tissues have helped address a broad range of questions in animal ecology. it follows that every tissue sample provides an isotopic profile that can be used to study dietary or movement histories of individual animals. interpretations of these profiles depend on the a ...201222631029
characterization of a functional serum response element in the actin403 gene promoter from the crustacean artemia franciscana.the serum response factor (srf) activates expression of several genes in response to growth factors present in serum. srf also regulates the expression of tissue-specific genes, including those in vertebrate muscles. an srf-binding site (carg box) present in the artemia franciscana actin403 promoter was shown to be necessary for transcriptional activity in cultured cells from drosophila melanogaster and mammals. this dna region bound mammalian and drosophila srfs in vitro and mediated transcript ...200111322877
cryopreservation of lipid bilayers by lea proteins from artemia franciscana and trehalose.the capacity of late embryogenesis abundant (lea) proteins and trehalose to protect liposomes against freezing-induced damage was examined by measuring the leakage of 5(6)-carboxyfluorescein (cf). liposomes were prepared to simulate the lipid compositions of the inner leaflet of the plasma membrane, outer mitochondrial membrane (omm), and inner mitochondrial membrane (imm). two recombinant lea proteins belonging to group 3 (afrlea2 and afrlea3m) were expressed and purified from embryos of artemi ...201627393243
isolation and characterization of the gene coding for artemia franciscana tata-binding protein: expression in cryptobiotic and developing embryos.genomic and cdna clones coding for the artemia franciscana homolog of the tata box-binding protein (tbp) were isolated. the c-terminal region of the predicted protein displays up to 92% sequence identity with the conserved c-terminal regions of tbps from other species. the gene is divided in seven exons that expand over a region of 33 kb. the position of the four introns located in the conserved c-terminal region has been compared with those of other species. two of these introns have been gener ...199910366711
cloning of a cdna encoding an artemia franciscana na/k atpase alpha-subunit.clones of cdna that code for an isoform of the artemia franciscana na/k atpase alpha subunit (naka alpha) have been isolated. the sequence of the longest of these clones (paratna136) is 3595 nucleotides; it codes for a 1004-amino acid protein whose sequence is identical to that of two previously sequenced artemia naka alpha peptides. the encoded protein is over 73% identical to drosophila melanogaster and vertebrate naka alpha s, and 73.8% identical to another artemia naka alpha isoform previous ...19911657719
concentrations of selenium and mercury in eared grebes (podiceps nigricollis) from utah's great salt lake, usa.we examined selenium and mercury concentrations in eared grebes (podiceps nigricollis) that spent the fall of 2006 on the great salt lake (ut, u.s.a.), where their diet consisted mainly of brine shrimp (artemia franciscana). selenium concentrations in livers varied based on when the grebes were collected (lower in september [mean +/- standard error, 9.4 +/- 0.7 microg/g dry wt] than in november [14.5 +/- 1.4 microg/g]), on where the birds were collected on the great salt lake (antelope island, 8 ...200919173549
in vitro and in vivo exploration of palmitic acid from synechococcus elongatus as an antibiofilm agent on the survival of artemia franciscana against virulent vibrios.biofilm formation of vibrio spp. has been demonstrated as a potentially important mechanism contributing antibiotic treatment failure in aquaculture. in the present study, the effect of palmitic acid (pa) identified from synechococcus elongatus was assessed for the inhibition of quorum sensing (qs) regulated biofilm formation in aquatic bacterial pathogens. the biofilm inhibitory concentration (bic) of pa against vibrio spp. was found to be 100µgml(-1). in this concentration, pa exhibited a sign ...201728887169
[evaluation of the toxicity of basidiomycota fungi on the hatching of artemia franciscana cysts].the consumption of wild mushrooms has increased in recent years. however, not all mushrooms are edible and some of them may cause poisoning. therefore, their toxicity needs to be studied. artemia franciscana is a crustacean used in toxicity tests including toxins of fungi.201728830736
assessing estuarine quality: a cost-effective in situ assay with amphipods.in situ assays based on feeding depression can be powerful ecotoxicological tools that can link physiological organism-level responses to population and/or community-level effects. amphipods are traditional target species for toxicity tests due to their high sensitivity to contaminants, availability in the field and ease of handling. however, cost-effective in situ assays based on feeding depression are not yet available for amphipods that inhabit estuarine ecosystems. the aim of this work was t ...201626874320
embryonic exposure to environmentally relevant concentrations of pcb126 affect prey capture ability of fundulus heteroclitus larvae.early life stages from a marine fish species, fundulus heteroclitus, were exposed to sublethal doses of 3,3',4,4',5 pentachlorobiphenyl (pcb126) to evaluate its effects on ecologically relevant responses: growth and behavior. a few hours after fertilisation, eggs were treated topically with pcb126 (2.5-50 pg egg⁻¹). four days post-hatching (dph), morphological changes (body length and malformations), spontaneous locomotor activity (active swimming speed, rate of travel, % inactivity), prey captu ...201121349578
nodularia spumigena peptides--accumulation and effect on aquatic invertebrates.thus far, the negative effects of nodularia spumigena blooms on aquatic organisms have been mainly attributed to the production of the hepatotoxic nodularin (nod). in the current work, the accumulation of other n. spumigena metabolites in blue mussels and crustaceans, and their effect on thamnocephalus platyurus and artemia franciscana, were examined. the liquid chromatography-tandem mass spectrometry (lc-ms/ms) analyses provided evidence that both blue mussels collected after a cyanobacterial b ...201526529012
bioenhancement of cadmium transfer along a multi-level food chain.previous studies have shown that metal partitioned to a subcellular compartment containing trophically available metal (tam) is readily available to predators and may be enhanced by increased binding of metal to heat-stable proteins (hsp - e.g., metallothioneins). the aim of the current investigation was to determine the influence of tam on the trophic transfer of cd along an experimental, three-level food chain: artemia franciscana (brine shrimp)-->palaemonetes pugio (grass shrimp)-->fundulus h ...200515603770
growth and lipid composition of atlantic cod (gadus morhua) larvae in response to differently enriched artemia franciscana.considerable progress has been achieved in the intensive culture of atlantic cod (gadus morhua). however, there is little information concerning optimum live-feed enrichments for cod larvae, since many of the techniques used during the larviculture have been borrowed from other fish species and adapted for the production of atlantic cod. the present study compared four different protocols for the enrichment of artemia to be used as live feed for cod larvae. the protocols tested were: (1) algamac ...200818649026
long-term toxicity of surface-charged polystyrene nanoplastics to marine planktonic species dunaliella tertiolecta and artemia franciscana.plastic pollution has been globally recognized as a critical issue for marine ecosystems and nanoplastics constitute one of the last unexplored areas to understand the magnitude of this threat. however, current difficulties in sampling and identifying nano-sized debris make hard to assess their occurrence in marine environment. polystyrene nanoparticles (ps nps) are largely used as nanoplastics in ecotoxicological studies and although acute exposures have been already investigated, long-term tox ...201728644993
terminalia ferdinandiana exell. extracts inhibit the growth of body odour-forming bacteria.terminalia ferdinandiana extracts are potent growth inhibitors of many bacterial pathogens. they may also inhibit the growth of malodour-producing bacteria and thus be useful deodorant components, although this is yet to be tested.201728488331
determination of the optimal enrichment artemia franciscana with a synbiotic combination of probiotics pediococcus acidilactici and prebiotic fructooligosaccharide.in this study the optimal amount of enriching artemia franciscana with a synbiotic combination of pediococcus acidilactici and fructooligosaccharide (fos( was investigated. the experiment was conducted in a completely randomized design in ten treatments: multi-level probiotics p. acidilactici (1×10(9), 1×10(8) and 1× 10(7) cfu per l) and multi-level enriched prebiotic fos (1, 2 and 5) g per l of solution and control. to evaluate the enrichment of adult artemia with each treatment, sampling was p ...201728473897
effect of methylparaben in artemia franciscana.in this study, the toxicity of methylparaben (mep) an emerging contaminant, was analysed in the sexual species artemia franciscana, due to its presence in coastal areas and marine saltworks in the mediterranean region. the acute toxicity (24h-lc50) of mep in nauplii was tested and its chronic effect (9days) evaluated by measuring survival and growth under two sublethal concentrations (0.0085 and 0.017mg/l). also, the effect on several key enzymes involved in: antioxidant defences (catalase (cat) ...201728428009
influence of surfactants and humic acids on artemia franciscana's embryonic phospho-metabolite profile as measured by (31)p nmr.surfactants, such as triton x-100 (tx-100), cetylpyridinium chloride (cpc), and sodium dodecyl sulfate (sds) are known to be toxic to artemia franciscana (artemia) - an organism, frequently used to monitor the health of the aquatic environment. the phospho-metabolite profile of a living organism is often indicative of imbalances that may have been caused by environmental stressors, such as surfactants. this study utilizes in vivo(31)p nmr to monitor temporal changes in the phospho-metabolite pro ...201728285196
molecular phylogeny and toxicity of harmful benthic dinoflagellates coolia (ostreopsidaceae, dinophyceae) in a sub-tropical marine ecosystem: the first record from hong kong.coolia are marine benthic dinoflagellates which are globally distributed and potentially toxic. this study provides the first investigation of species diversity and toxicity assessment of coolia in hong kong waters. fifty-one strains of four coolia species, including c. malayensis, c. canariensis, c. tropicalis, and c. palmyrensis, were isolated from twelve sub-tidal habitats, and identified phylogenetically using 28s rdna sequences. exposure experiments (48-hour) demonstrated that the algal lys ...201728139234
yellow-emitting carbon nanodots and their flexible and transparent films for white leds.we report carbon nanodots that can be utilized as effective color converting phosphors for the production of white light-emitting diodes (leds). blue-excitable and yellow-emitting carbon nanodots, functionalized with 3-(imidazolidin-2-on-1-yl)propylmethyldimethoxysilane (ipmds)-derived moieties (is-cds), are synthesized by a novel one-pot reaction in which the products from the initial reaction occurring between urea and 3-(2-aminoethylamino)propylmethyl-dimethoxysilane (aepmds) are further trea ...201627934198
defense systems in developing artemia franciscana nauplii and their modulation by probiotic bacteria offer protection against a vibrio anguillarum challenge.the alterations of immune responses of artemia franciscana nauplii as a function of culture time and after a challenge with the pathogen vibrio anguillarum were studied. the effect of the administration of the probiotic bacteria bacillus subtilis, lactobacillus plantarum and lactococcus lactis either alone or in combination with the pathogen was evaluated. the activity of the antioxidant enzymes superoxide dismutase (sod), glutathione reductase (gred), glutathione transferase (gst) and phenoloxi ...201728478257
comparing cestode infections and their consequences for host fitness in two sexual branchiopods: alien artemia franciscana and native a. salina from syntopic-populations.the american brine shrimp artemia franciscana is invasive in the mediterranean region where it has displaced native species (the sexual a. salina, and the clonal a. parthenogenetica) from many salt pond complexes. artemia populations are parasitized by numerous avian cestodes whose effects have been studied in native species. we present a study from the ebro delta salterns (ne spain), in a salt pond where both a. franciscana and native a. salina populations coexist, providing a unique opportunit ...201526157636
the total and mitochondrial lipidome of artemia franciscana encysted embryos.encysted embryos (cysts) of the crustacean artemia franciscana exhibit enormous tolerance to adverse conditions encompassing high doses of radiation, years of anoxia, desiccation and extreme salinity. so far, several mechanisms have been proposed to contribute to this extremophilia, however, none were sought in the lipid profile of the cysts. here in, we used high resolution shotgun lipidomics suited for detailed quantitation and analysis of lipids in uncharacterized biological membranes and sam ...201627542539
surfactant toxicity to artemia franciscana and the influence of humic acid and chemical composition.surfactants can be extremely toxic to aquatic species and are introduced to the environment in a variety of ways. it is thus important to understand how other environmental constituents, in this case humic acids (has), may alter the toxicity of anthropogenic surfactants. hatching and mortality assays of artemia franciscana were performed for three different toxic surfactants: triton x-100 (tx-100, non-ionic), cetylpyridinium chloride (cpc, cationic), and sodium dodecyl sulfate (sds, anionic). hu ...201727453688
high performance liquid chromatography-mass spectrometry analysis of high antioxidant australian fruits with antiproliferative activity against cancer cells.high antioxidant capacities have been linked to the treatment and prevention of several cancers. recent reports have identified several native australian fruits with high antioxidant capacities. despite this, several of these species are yet to be tested for anticancer activity.201627279705
biochemical and toxicological effects of organic (herbicide primextra(®) gold tz) and inorganic (copper) compounds on zooplankton and phytoplankton species.in europe, mainly in the mediterranean region, an intensive usage of pesticides was recorded during the past 30 years. according to information from agricultural cooperatives of the mondego valley (figueira da foz, portugal), primextra(®) gold tz is the most used herbicide in corn crop fields and one of the 20 best-selling herbicides in portugal. copper is mainly used in pesticide formulations. this study aims to determine the ecotoxicological and biochemical (namely fatty acid profiles) effects ...201627239776
impacts of harvesting on brine shrimp (artemia franciscana) in great salt lake, utah, usa.selective harvesting can cause evolutionary responses in populations via shifts in phenotypic characteristics, especially those affecting life history. brine shrimp (artemia franciscana) cysts in great salt lake (gsl), utah, usa are commercially harvested with techniques that select against floating cysts. this selective pressure could cause evolutionary changes over time. our objectives are to (1) determine if there is a genetic basis to cyst buoyancy, (2) determine if cyst buoyancy and nauplii ...201627209783
gamma radiation reduced toxicity of azoxystrobin tested on artemia franciscana.fungicide azoxystrobin toxicity was monitored by means of a 96-h biotest with artemia franciscana nauplius stages after exposure to solutions with concentrations of 0.2, 0.4, 0.6 and 0.8 mg l(-1) irradiated with (60)co gamma radiation with doses of 1, 2.5, 5 and 10 kgy. the effects of ionization radiation on azoxystrobin toxicity were mainly manifested by a statistically significant reduction of lethality after 72- and 96-h exposure. a maximum reduction of lethality of 72 % was achieved using do ...201627107585
remediation of mercury contaminated saltwater with functionalized silica coated magnetite nanoparticles.the study aimed to evaluate the efficiency of dithiocarbamate functionalized silica coated magnetite nanoparticles (nps) for hg decontamination of saltwater either contaminated with hg alone or with as and cd. for this, the residual levels of hg in seawater were assessed and hg-contaminated or hg+as+cd-contaminated seawater toxicity to aquatic biota, before and after the sorption process, was compared. the results showed that under highly competitive conditions (water salts, cd and as), the remo ...201627039062
salt sensitivity of the morphometry of artemia franciscana during development: a demonstration of 3d critical windows.a 3d conceptual framework of 'critical windows' was used to examine whether the morphometry of artemia franciscana is altered by salinity exposure during certain key periods of development. artemia franciscana were hatched at 20 ppt (designated control salinity) and were then exposed to 10, 30, 40 or 50 ppt either chronically (days 1-15) or only on days 1-6, 7-9, 10-12 or 13-15. on day 15, maturity was assessed and morphometric characteristics, including mass, total body length, tail length and ...201626685168
liposomes with diverse compositions are protected during desiccation by lea proteins from artemia franciscana and trehalose.intracellular accumulation of late embryogenesis abundant (lea) proteins and the disaccharide trehalose is associated with cellular desiccation tolerance in a number of animal species. two lea proteins from anhydrobiotic embryos of the brine shrimp artemia franciscana were tested for the ability to protect liposomes of various compositions against desiccation-induced damage in the presence and absence of trehalose. damage was assessed by carboxyfluorescein leakage after drying and rehydration. f ...201626518519
effects of rearing density on survival, growth, and development of the ladybird coleomegilla maculata in culture.our research focuses on developing techniques to rear ladybird beetles (coleoptera: coccinellidae). we evaluated the effects of rearing density on survival, growth, and development of coleomegilla maculata. the hypothesis that a low to moderate rearing density has limited or no effects on survival and development was tested. c. maculata first instars were reared to pupae at a density of 1, 5, 10, 15, or 20 individuals per arena (2.5 cm high, 9.0 cm diameter, and 159 cm3 volume) and fed powdered ...201526466904
does a change from whole to powdered food (artemia franciscana eggs) increase oviposition in the ladybird coleomegilla maculata?the limited availability of alternative foods to replace natural prey hinders cost-effective mass production of ladybird beetles for augmentative biological control. we compared the effects of powdered vs. whole artemia franciscana (a. franciscana) (brine shrimp) eggs with or without a dietary supplement on development and reproduction of coleomegilla maculata (c. maculata) (coleoptera: coccinellidae). we tested the hypotheses that (1) powdered a. franciscana eggs are more suitable than whole eg ...201526466902
nano-sized polystyrene affects feeding, behavior and physiology of brine shrimp artemia franciscana larvae.nano-sized polymers as polystyrene (ps) constitute one of the main challenges for marine ecosystems, since they can distribute along the whole water column affecting planktonic species and consequently disrupting the energy flow of marine ecosystems. nowadays very little knowledge is available on the impact of nano-sized plastics on marine organisms. therefore, the present study aims to evaluate the effects of 40nm anionic carboxylated (ps-cooh) and 50nm cationic amino (ps-nh2) polystyrene nanop ...201626422775
lethal and sublethal endpoints observed for artemia exposed to two reference toxicants and an ecotoxicological concern organic compound.swimming speed alteration and mortality assays with the marine crustacean artemia franciscana were carried out. ec50 and lc50 values after 24-48h exposures were calculated for two reference toxicants, copper sulphate pentahydrate (cuso4·5h2o) and sodium dodecyl sulphate (sds), and an ecotoxicological concern organic compound, diethylene glycol (deg). different end-points have been evaluated, in order to point out their sensitivity levels. the swimming speed alteration (ssa) was compared to morta ...201626344887
physiological strategies during animal diapause: lessons from brine shrimp and annual killifish.diapause is a programmed state of developmental arrest that typically occurs as part of the natural developmental progression of organisms that inhabit seasonal environments. the brine shrimp artemia franciscana and annual killifish austrofundulus limnaeus share strikingly similar life histories that include embryonic diapause as a means to synchronize the growth and reproduction phases of their life history to favorable environmental conditions. in both species, respiration rate is severely dep ...201526085666
enhancing the efficacy of electrolytic chlorination for ballast water treatment by adding carbon dioxide.we examined the synergistic effects of co2 injection on electro-chlorination in disinfection of plankton and bacteria in simulated ballast water. chlorination was performed at dosages of 4 and 6ppm with and without co2 injection on electro-chlorination. testing was performed in both seawater and brackish water quality as defined by imo g8 guidelines. co2 injection notably decreased from the control the number of artemia franciscana, a brine shrimp, surviving during a 5-day post-treatment incubat ...201525841887
maternal transfer of inorganic mercury and methylmercury in aquatic and terrestrial arthropods.the transfer of mercury from females to their offspring plays an important role in mercury accumulation and toxicity during early development. to quantify the transfer of inorganic mercury and methylmercury from female arthropods to their eggs, the authors collected and analyzed brine shrimp (artemia franciscana), wolf spiders (alopecosa spp.), and their attached eggs from aquatic and terrestrial ecosystems at the great salt lake, utah, usa. essentially all of the mercury in both the female brin ...201323939924
functional differentiation of small heat shock proteins in diapause-destined artemia embryos.encysted embryos of artemia franciscana cease development and enter diapause, a state of metabolic suppression and enhanced stress tolerance. the development of diapause-destined artemia embryos is characterized by the coordinated synthesis of the small heat shock proteins (shsps) p26, arhsp21 and arhsp22, with the latter being stress inducible in adults. the amounts of shsp mrna and protein varied in artemia cysts, suggesting transcriptional and translational regulation. by contrast to p26, kno ...201323879561
effects of alginate on stability and ecotoxicity of nano-tio2 in artificial seawater.the large-scale use of titanium dioxide nanoparticles (nano-tio₂) in consumer and industrial applications raised environmental health and safety concerns. potentially impacted ecosystems include estuarine and coastal organisms. results from ecotoxicological studies with nano-tio₂ dispersed in salt exposure media are difficult to interpret due to fast flocculation and sedimentation phenomena affecting the dispersion stability. the goal of this study was to investigate the stabilisation effect of ...201525841066
toxicity of two imidazolium ionic liquids, [bmim][bf4] and [omim][bf4], to standard aquatic test organisms: role of acetone in the induced toxicity.the main goal of this study was to investigate the toxicity of the imidazolium-based ionic liquids (ils), [bmim][bf4] (1-butyl-3-methylimidazolium tetrafluoroborate) and [omim][bf4] (1-octyl-3-methylimidazolium tetrafluoroborate), in battery of standard aquatic toxicity test organisms. specifically, exposure of the algae scenedesmus rubescens, crustaceans thamnocephalus platyurus and artemia franciscana, rotifers brachionus calyciflorus and brachionus plicatilis and bivalve mytilus galloprovinci ...201525839183
highly repetitive dna sequence in parthenogenetic artemia.the study of the structural organization of the eukaryotic genome is one of the most important tools for disclosing the evolutionary relationships between species. artemia (crustacea, phyllopoda) offers a very interesting model for speciation studies. the genus, distributed all over the world, comprises both bisexual sibling species and parthenogenetic populations, exhibiting different chromosome numbers (diploidy, polyploidy, and heteroploidy). digestion of genomic dna of the parthenogenetic ar ...19911901366
metabolic downregulation and inhibition of carbohydrate catabolism during diapause in embryos of artemia franciscana.diapause embryos were collected from ovigerous females of artemia franciscana at the great salt lake, utah, and were synchronized to within 4 h of release. respiration rate for these freshly released embryos across a subsequent 26-d time course showed a rapid decrease during the first several days followed thereafter by a much slower decline. the overall metabolic depression was estimated to be greater than 99%. however, proton conductance of mitochondria isolated from diapause and postdiapause ...201323303325
potential effects of tio2 nanoparticles and ticl4 in saltwater to phaeodactylum tricornutum and artemia franciscana.nanosized titanium dioxide (ntio2) is widespread in many commercial products and several authors investigated its ecotoxicity effects focusing mainly on freshwater environments. data on saltwater species are still lacking or present contradicting results. we compared for the first time the toxicity of ticl4 and ntio2 considering standard toxicity tests with microalgae phaeodactylum tricornutum (growth inhibition test, 1.8-90mg/l) and crustacean artemia franciscana (mortality test, 0.5-64mg/l). f ...201727913027
arhsp40, a type 1 j-domain protein, is developmentally regulated and stress inducible in post-diapause artemia franciscana.upon diapause termination and exposure to favorable environmental conditions, cysts of the crustacean artemia franciscana reinitiate development, a process dependent on the resumption of metabolic activity and the maintenance of protein homeostasis. the objective of the work described herein was to characterize molecular chaperones during post-diapause growth of a. franciscana. an hsp40 complementary dna (cdna) termed arhsp40 was cloned and shown to encode a protein with an amino-terminal j-doma ...201627581971
non-lethal heat shock increases tolerance to metal exposure in brine shrimp.pollution and temperature increase are two of the most important stressors that aquatic organisms are facing. exposure to elevated temperatures and metal contamination both induce heat shock proteins (hsps), which may thus be involved in the induced cross-tolerance in various organisms. this study aimed to test the hypothesis that exposure to a non-lethal heat shock (nlhs) causes an increased tolerance to subsequent metal exposure. using gnotobiotic cultures of the brine shrimp artemia francisca ...201627619211
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