Publications

TitleAbstractYear(sorted ascending)
Filter
PMID
Filter
immunological regulation of angiostrongylus cantonensis infections in rats: modulation of population density and enhanced parasite growth following one or two superimposed infections.rats acquired a degree of protective immunity to reinfection with angiostrongylus cantonensis after a single infection with 50 infective larvae. infected rats resisted the establishment of most challenging larvae and protective immunity increased with subsequent reinfections. part of the primary infection was lost after a superimposed second and also following a superimposed third infection, but the total size of the concurrent adult worm populations remained the same as that from a primary infe ...19836875253
studies on eosinophilic meningitis and meningoencephalitis caused by angiostrongylus cantonensis in japan.twenty cases of eosinophilic meningitis or meningoencephalitis suspected of being angiostrongyliasis were recognized in japan, including 16 cases in okinawa prefecture. among the cases, there was only one proven case in okinawa. the disease evenly occurred throughout all seasons. both sexes were equally affected, but 17 cases (more than 80%) occurred among adults. intentional ingestion of various mollusks and fresh livers of toads for therapeutic reasons were interestingly responsible for 7 case ...19836424245
the distribution of angiostrongylus cantonensis (chen) in the central nervous system of laboratory rats.the distribution of preadult angiostrongylus cantonensis helminths in various regions of the central nervous system of rats, infected with varying doses of larvae, was studied. a dose of 10 000 larvae proved to be lethal; the rats showed paralytic symptoms and loss of vision prior to death. a very low dose of 15 larvae also proved to be infective to the central nervous system. incidence of eye involvement was observed in two rats receiving a dose of 500 larvae. maximum number of helminths were r ...19836629208
a comparison of the effects of flubendazole and thiabendazole on the larvae of angiostrongylus cantonensis, trichinella spiralis, diphyllobothrium erinacei and hymenolepis nana in mice.flubendazole or thiabendazole were administered orally to mice harbouring larvae of angiostrongylus cantonensis, trichinella spiralis, diphyllobothrium erinacei or hymenolepis nana 5-10, 28-33, 21-26 and 1-6 days post-infection respectively. all the mice infected with a. cantonensis were completely cured after treatment with flubendazole at 5 mg/kg/day for 6 days. no noticeable damage was found in the cerebral hemispheres of the mice treated with flubendazole and examined under a dissecting micr ...19836657308
studies on chemotherapy of parasitic helminths: effects of avermectin b1a on angiostrongylus cantonensis in rats. 19836685712
studies on the cryopreservation of eggs of angiostrongylus cantonensis.the possibility of cryopreserving the eggs of angiostrongylus cantonensis collected from the uterus of female worms was investigated. eggs were cultured in nctc 109 medium containing 50% rat serum, and various growth stages, from one-cell eggs to embryonated eggs, were used in this study. as a cryoprotective agent, dimethylsulphoxide (me2so) was added to the medium at a final concentration of 1 m. eggs suspended in 0.2 ml of the medium at 37 degrees c were cooled to 0 degrees c at a rate of 1 de ...19836686843
studies on chemotherapy of parasitic helminths (xviii). mechanism of spastically paralyzing action of pyrantel in angiostrongylus cantonensis.pyrantel tartrate caused spastic paralysis through stimulating nicotinic cholinoceptors in angiostrongylus cantonensis.19836653728
protection against pasteurella pneumotropica during infection with angiostrongylus cantonensis.lung infections of specific-pathogen-free rats were studied to determine cross-immunity between infections with pasteurella pneumotropica and angiostrongylus cantonensis. rats were found to have differences in mortality due to pneumonic infections with p pneumotropica and a cantonensis and to coinfections of p pneumotropica and a cantonensis. single infections of p pneumotropica were not dosage-dependent in their lethality for specific-pathogen-free rats. the mortality of coinfection with p pneu ...19836226222
[fatal infection of hamsters (mesocricetus auratus) with angiostrongylus cantonensis (chen, 1935)]. 19836379767
the freshwater ampullariid snail lanistes carinatus as a natural intermediate host of angiostrongylus cantonensis in egypt. 19836631087
peripheral blood white cell responses during angiostrongylus cantonensis infection in rats. 19846735584
[infestation by angiostrongylus cantonensis in the havana provinces]. 19846390581
angiostrongylus cantonensis: paralysis due to avermectin b1a and ivermectin.paralysis due to avermectin b1a and ivermectin of angiostrongylus cantonensis was compared to that of phenylephrine (an alpha-adrenergic agonist) and strychnine (a cholinergic inhibitor). the paralyzing action of ivermectin (2.5 x 10(-9) g/ml) was inhibited by the single, simultaneous addition of picrotoxin (3 x 10(-5) m), whereas the effect of the drug (2.5 x 10(-7) g/ml) was reversed only when picrotoxin was given with cholinergic spasmogens such as pyrantel and eserine. bicuculline (3 x 10(-5 ...19846143682
circumoval and circumlarval precipitate reactions of angiostrongylus cantonensis. 19846716749
[a survey of angiostrongylus cantonensis in guangzhou]. 19846467537
the presence of angiostrongylus cantonensis in viti levu, fiji.wild rats and molluscs were examined for angiostrongylus cantonensis infection on viti levu, fiji. a. cantonensis were recovered from 29.6% (16/54) of rattus rattus and 59.5% (25/42) of r. exulans. a. cantonensis-like larval nematodes were found in all of four slugs, laevicaulis alte, and ten of 20 unidentified land snails. the larvae developed to adult a. cantonensis in the pulmonary arteries of laboratory rats 40 to 42 days after ingestion. this is the first record of a. cantonensis in fiji.19846501855
studies of angiostrongylus cantonensis in two experimental hosts.lung infection with angiostrongylus cantonensis was established in experimentally infected indian soft-furred rats (millardia meltada) and indian spiny mice (mus platythrix). pulmonary arterial migration occurred earlier (at day 20 after infection) in m. meltada than in wistar rats. m. meltada and m. platythrix could serve as useful animal models for comparative studies of mechanisms determining host specificity of a. cantonensis.19846501859
angiostrongylus cantonensis in east new britain, papua new guinea.the first case of angiostrongyliasis (eosinophilic meningitis) to be diagnosed in papua new guinea in 1977, led to a survey of adult melanesian residents of east new britain province where the case occurred, to assess the level of immunological responsiveness to angiostrongylus cantonensis. in 104 subjects tested using an indirect fluorescent antibody test, the serum antibody titres to a. cantonensis equalled or exceeded 1:16 in 50.7% and ranged from 1:32 to 1:64 in 17.3% of subjects. no individ ...19846533849
distribution of angiostrongylus cantonensis in papua new guinea.examination of 97 rats trapped in port moresby and lae on the mainland of papua new guinea and in rabaul, east new britain province, showed that 12.4% were infected by angiostrongylus cantonensis. larval an. cantonensis were found in achatina fulica snails collected at these towns, from the ramu valley near madang on the mainland and at locations in west new britain, new ireland and bougainville island. the mollusc infection rates ranged from 12.7% at port moresby to 32.4% at kimbe in west new b ...19846533850
parasite density and the fecundity of angiostrongylus cantonensis in rats.the larval output of angiostrongylus cantonensis at different initial densities in rats was examined throughout the reproductive life-span of the worms using a modified dilution technique. with an initial density of 2 worms the mean duration of larval output was 414.2 days. the output was at a level above 50000 larvae/female/day from 15 to 45 weeks after infection. the mean total output was 18.9 x 10(6) larvae/female. the larval output/female decreased with an increase in the worm burden and als ...19846504559
[the infection of angiostrongylus cantonensis in definitive and intermediate hosts in guangzhou 1979-1982]. 19846509537
the chronic and severe forms of eosinophilic meningitis.two cases of chronic eosinophilic meningitis (presumed to be due to angiostrongylus cantonensis) and a third patient with a severe form of this disease are described. such cases are rare in the published literature. all patients developed their illness in western samoa, an area previously thought to be free of the disease. the need to consider eosinophilic meningitis in patients with chronic and severe neurological disease who live in, or have recently come from, endemic areas is emphasised.19846591910
ultrastructural evidence for eosinophil-mediated destruction of angiostrongylus cantonensis transferred into the pulmonary artery of non-permissive hosts.ultrastructural and cytochemical analyses were carried out on cellular reactions to the young adult worms of angiostrongylus cantonensis surgically transferred into the pulmonary arteries of permissive (rat) and non-permissive (rabbit and guinea-pig) hosts. in permissive hosts, no appreciable cellular reactions could be found around worms throughout the course of the observations. by contrast, the infiltration of neutrophils along with eosinophils was observed around worms in non-permissive host ...19846718049
effects of temperature on the larval development of angiostrongylus cantonensis in the intermediate host, biomphalaria glabrata.the effects of temperature on the larval development of angiostrongylus cantonensis in biomphalaria glabrata were studied under controlled conditions. when b. glabrata were maintained at different, constant temperatures, the first-stage larvae developed to third-stage larvae between 20 degrees and 31 degrees c. the velocity of development to the third-stage in the snail depended on the relationship, y = -0.1281 + 0.0081 x (y velocity of development; x rearing temperature). the "threshold of deve ...19846741223
an examination of the prepatent period and the absence of a crowding effect in angiostrongylus cantonensis in laboratory rats.the effects of the size of the infective inoculum on the prepatent period and the effect of crowding on body volume were examined in populations of angiostrongylus cantonensis (metastrongyloidea: angiostrongylidae) in the laboratory rat. groups of five rats were infected with 5, 10, 20 and 40 l3 of a. cantonensis, respectively. the prepatent period was determined by examination of faeces using a modified baermann technique. the prepatent period ranged from 37 to 45 days and was best described by ...19853836309
[experimental infection of angiostrongylus cantonensis larvae to ampullarium canaliculatus]. 19853858429
elisa testing in cases of clinical angiostrongyliasis in thailand.the sera from 116 thais admitted to nakhon ratchasima hospital in northeastern thailand with eosinophilic meningitis were tested for antibodies to angiostrongylus cantonensis by elisa. ninety-six percent of the sera were considered positive with elisa values exceptionally high for most patients. the clinical aspects of the disease are also presented. the elisa test is considered to be of value in the diagnosis of the disease but tests for the antigen would provide a more definitive diagnosis.19854023800
spread of angiostrongylus cantonensis in papua new guinea. 19853866437
polyclonal b cell activation during the course of angiostrongylus cantonensis infection.the numbers of igm antibody forming cells against various heterologous antigens: srbc, hrbc, tnp and fitc, were found to be elevated in the spleens of rats after infection with angiostrongylus cantonensis. in these rats, the number of splenic b cells and total igg and igm levels in serum also increased spontaneously about 2- to 3-fold of uninfected rats during the course of the infection. these results suggested that a. cantonensis infection may activate b cells polyclonally in the spleens of th ...19853876249
induction of protective immunity against infection by angiostrongylus cantonensis in rats.attempts were made to induce acquired immunity against angiostrongylus cantonensis infection by immunizing rats with somatic (l3s) and metabolic (l3es) antigens from third-stage larvae and with somatic antigen from adult female worms (face) via either a combined intraperitoneal and oral (ip-or-or) or subcutaneous (sc-sc-sc) route. the most effective mean in inducing protection was the administration of l3s by the ip-or-or route, as judged from reduced mortality, prolonged survival periods and/or ...19854023814
larvicidal effect of flubendazole on angiostrongylus cantonensis in mice with various worm burdens.flubendazole given orally at 50 mg/kg to mice inoculated with 50, 250 or 500 larvae of angiostrongylus cantonensis showed almost complete larvicidal effect, irrespective of the worm burden.19854093587
isolation of specific antigens from angiostrongylus cantonensis. 1. preparative flatbed isoelectric focusing.electrophoresis on sds gel and analytical isoelectric focusing showed that a crude extract of angiostrongylus cantonensis consisted of at least 40 protein components with molecular weights ranging from 13 000-70 000 and isoelectric points of pi values ranging from 3.7-10.0. crossed-immunoelectrophoresis with a hyperimmune antiserum to a. cantonensis showed at least 40 different antigenic components in the crude worm extract which were cross-reactive with those of ascaris suum, metastrongylus apr ...19852417425
isolation of specific antigens from angiostrongylus cantonensis. 2. by affinity chromatography.the crude adult worm extract of angiostrongylus cantonensis was subjected to a series of affinity chromatography for selective removal of host antigens as well as cross-reactive components with other helminths. the purified fraction designated as ac(p) with molecular weights ranging from 10,000-42,000 was found to contain antigenic components specific to a. cantonensis as determined by immunoelectrophoresis and enzyme-linked immunosorbent assay.19862428179
[technic for the collection of larvae of angiostrongylus cantonensis from mollusca]. 19863299555
[epidemiology of angiostrongylus cantonensis in guangzhou]. 19863521887
demonstration of carboxyl and thiol protease activities in adult schistosoma mansoni, dirofilaria immitis, angiostrongylus cantonensis and ascaris suum.evidence has been presented showing two kinds of acidic protease activities in adult schistosoma mansoni, dirofilaria immitis, angiostrongylus cantonensis and ascaris suum. a haemoglobinolytic activity without adding any sh-containing compounds was maximal at ph 3.5, 2.5, 3.0 and 3.5 in s. mansoni, d. immitis, angiostrongylus cantonensis and ascaris suum respectively. the inhibitor studies demonstrated that this activity is ascribable to carboxyl protease(s). in the presence of dithiothreitol, a ...19863517135
surface antigens of angiostrongylus cantonensis developing in permissive and non-permissive hosts.the immunofluorescent antibody test and immunocytochemical method were employed to study the surface antigens of angiostrongylus cantonensis obtained from infected rats, mice and guinea pigs. positive results with intense fluorescence and brownish peroxidase staining were observed on the cuticular surface of a. cantonensis recovered from rats 22 days (late cerebral phase) and 34 days (lung phase) post-infection when tested with antisera against host (normal rat serum) antigens as well as crude e ...19863529674
enzyme-linked immunosorbent assay for detection of antibody to gnathostoma antigen in patients with intermittent cutaneous migratory swelling.a sensitive enzyme-linked immunosorbent assay is described for the detection of immunoglobulin g antibody to gnathostoma antigen in the sera of patients with intermittent cutaneous migratory swelling who were suspected of being infected by the tissue nematode gnathostoma spinigerum. the antigen used was a crude somatic aqueous extract of the third larval stage obtained from naturally infected eels. the 1:320 dilution of all sera from 46 patients gave enzyme-linked immunosorbent assay values abov ...19863711272
enzyme-linked immunosorbent assay (elisa) for the detection of antibodies to angiostrongylus cantonensis.the enzyme-linked immunosorbent assay (elisa) was employed for the sero-diagnosis of angiostrongylus cantonensis infections in rats and man. metabolic and 'purified' antigens of adult or juvenile a. cantonensis were evaluated by elisa and compared against toxocara canis antigen for sensitivity and specificity. sera and cerebrospinal fluid (csf) from both rats and man gave higher elisa values against antigens of a. cantonensis than those obtained from negative control samples. the results showed ...19863798533
effect of uv-irradiation, spleen cells and mln cells on protective immunity against angiostrongylus cantonensis infection in mice.the uv-irradiated larvae of angiostrongylus cantonensis were used for inducing immunity in mice. a single oral dose of 100 infective uv-irradiated larvae exposed for 5 min or 15 min induced 91% and 97% protection against subsequent infection. adoptive protection against a. cantonensis could also be transferred by spleen and mesenteric lymph node cells obtained from vaccinated mice.19863705711
infectivity and development of the gamma-irradiated first stage larvae of angiostrongylus cantonensis.effects of gamma-ray irradiation on the first-stage larvae of angiostrongylus cantonensis were studied. there was a significant reduction in the infectivity of the 10,000 r-irradiated larvae to intermediate host snails compared to other groups. in the final host infection, both the worm populations and worm body length of irradiated groups differed markedly from those of the non-irradiated control.19863716541
angiostrongylus cantonensis infection in ampullarius canaliculatus (lamarck) in kyushu, japan.ampullarius canaliculatus is a large fresh-water snail of which the original habitat is said to be argentina. recently, the snail which grew wild has propagated in various parts of japan, and is causing damage to aquatic plants by feeding on them. the present study was performed to find out whether the snail can be a suitable intermediate host for angiostrongylus cantonensis. natural infection with a. cantonensis was confirmed in the snails collected from 5 different places in 4 hamlets in okina ...19863576290
effects of diethylcarbamazine on the motility of angiostrongylus cantonensis and dirofilaria immitis.effects of piperazine derivatives, especially of diethylcarbamazine (dec) on adult angiostrongylus cantonensis and dirofilaria immitis were examined. piperazine (3 x 10(-5)-10(-4) m) paralyzed a. cantonensis and the action was antagonized by picrotoxin. 1,1-dimethyl-4-phenylpiperazinium iodide (dmpp) (10(-5)-10(-4) m) caused contraction but little effect was produced by strychnine. an inhibitory effect on untreated preparations was caused by lower concentrations (3 x 10(-6)-10(-5) m) of diethylc ...19863716544
eosinophil responses of permissive and nonpermissive hosts to the young adult worms of angiostrongylus cantonensis.blood and bone marrow eosinophilia was assessed in nonpermissive (guinea pigs) and permissive (rats) hosts following the pulmonary arterial transfers of live or dead young adult worms of angiostrongylus cantonensis. guinea pigs showed a marked eosinophilic response to live worms but only a slight response to dead worms. neither ige nor haemagglutinating antibodies correlated with the induction of this eosinophilia. in contrast, the rat responded to neither form of the young adult worm. when the ...19863776318
studies on anthelmintic effects of flubendazole and mebendazole on the rat lungworm angiostrongylus cantonensis in mice and rats.flubendazole and mebendazole given orally at 10 mg/kg/day 5-7 days post-infection (total 30 mg/kg) were found to eliminate 93-100% of angiostrongylus cantonensis larvae in mice and rats. no significant difference was observed between the effects of the 2 drugs. the effectiveness of the drugs decreased with the increase in days post-infection on which medication was administered. it was found possible to treat a. cantonensis adults in rats by administering flubendazole or mebendazole at 10 mg/kg/ ...19863783345
first report of angiostrongylus cantonensis in puerto rico.from january to april 1984, 63 rattus rattus and 40 r. norvegicus were trapped in northeastern puerto rico and examined for angiostrongylus cantonensis adults. nineteen (47.5%) of the r. norvegicus and 10 (15.9%) of the r. rattus were infected, giving an overall infection rate of 28.2%. four species of terrestrial snails and one species of brown slug were examined for a. cantonensis larvae. two snail species, subulina octona and aquebana belutina, were found infected with third stage larvae of a ...19863953946
four documented cases of eosinophilic meningoencephalitis due to angiostrongylus cantonensis in hong kong.4 cases of eosinophilic meningoencephalitis in hong kong are described. the major clinical features of the patients, who were 2-60 years old, were low grade fever, headache, mild meningeal signs, right facial palsy or hemiplegia. eosinophilia in the peripheral blood and eosinophilic pleocytosis were prominent. computerized tomography scans of brain showed a small area of attenuation with surrounding hypodense area; the lesion was resolved 1-2 months after admission to hospital. electroencephalog ...19873450002
studies on growth and distribution of angiostrongylus cantonensis larvae in ampullarium canaliculatus.the intramolluscan development of angiostrongylus cantonensis larvae in ampullarium canaliculatus, a fresh-water migrant snail which multiplies in tremendous rate in taiwan, was followed for 28 consecutive days in this study. the snails were infected with first stage larvae of a. cantonensis and groups of three snails were sacrificed daily to study various larval stages in different organs. a. cantonensis larvae were found in all snails examined. during the first four days, they were obtained on ...19873672187
canine neural angiostrongylosis: the clinical and therapeutic features of 55 natural cases.the clinical features of 55 naturally occurring cases of neural angiostrongylosis caused by the parasite angiostrongylus cantonensis are described. the principal clinical feature is an ascending paresis of varying severity, mainly effecting the limbs and bladder. in severely affected animals the cranial nerves and central nervous system are affected. a distinguishing clinical sign is a variable severe lumbar hyperalgesia. for prognostic purposes the severity of the disease is divided into 3 grad ...19873675410
pathogenic factors in the later pulmonary phase of angiostrongylus cantonensis-infected rats.in rats infected with different worm burdens of angiostrongylus cantonensis, the loss of body weight was proportional to the increasing dose of infection. body weight was correlated inversely with female worm burden (r = -0.669), the sum of mean numbers of larvae per gram of faeces (lpg) (r = -0.527) and lung weight (r = -0.761). lung weight was positively correlated with female worm burden (r = 0.785) and the sum of mean numbers of lpg (r = 0.685). on the other hand, in rats infected with larva ...19873658975
observations on the transmission of angiostrongylus cantonensis from snail to rodent.a survey of angiostrongylus cantonensis was carried out to investigate the mode of transmission from mollusc to rat in a fixed study area of yoron island from 1979 to 1982. rattus rattus was found to be infected with a small number of worms in spite of heavy infection with third-stage larvae in achatina fulica and an abundance of this snail in the area. natural infection and/or susceptibility with a. cantonensis were confirmed in three small snail species. bradybaena circulus, fruticicola despec ...19873668215
late development and fertility of adult worms derived from gamma-irradiated first-stage larvae of angiostrongylus cantonensis.effects of gamma ray irradiation on the first-stage larvae of angiostrongylus cantonensis were studied. compared to the non-treated controls, infection of rats with third-stage larvae which developed from irradiated first-stage larvae resulted in reduced recovery rates of adults. there was also a change in the male-female adult worm ratio and a reduction in larval output per female in relation to increasing irradiation dosage. morphological changes in the adults were noted.19873575290
larvicidal effect of perbendazole on experimental infection of angiostrongylus cantonensis in mice.perbendazole was given orally and subcutaneously to mice infected with angiostrongylus cantonensis at different stages of infection. the subcutaneous route of administration was more effective than the oral one. on the 5th day after infection, the perbendazole had a higher efficacy than on the 10th day postinfection. this finding shows that perbendazole had complete larvicidal effect at early stages of infection.19873428011
motility and drug susceptibility of angiostrongylus cantonensis developing from gamma-irradiated first-stage larvae.the effects of neuropharmacological agents on the motility of irradiated and non-irradiated angiostrongylus cantonensis adult females were studied. gaba induced complete paralysis in non-irradiated and 5,000 r-irradiated worms, but caused only slight paralysis on 10,000 r-irradiated worms. the paralytic effect of gaba was antagonised by picrotoxin. the reason for low susceptibility of heavily irradiated worms to gaba is not known. there was no difference in susceptibility of non-irradiated and i ...19873448779
effects of milbemycin d on adult angiostrongylus cantonensis in rats.effects of milbemycin d against adult angiostrongylus cantonensis in rats were examined. the first-stage larval counts in rat faeces (larvae per gram of faeces per female worm recovered, lpg/female) were most conspicuously reduced in the group treated with nine consecutive weekly doses of 5.0 mg/kg. the effect was more marked in the group treated with five or ten successive daily doses of 5.0 mg/kg than the group treated with a single dose of 25.0 or 5.0 mg/kg. host lung-body weight ratio and nu ...19873615397
experimental chemotherapy of angiostrongylus cantonensis infection in mice with flubendazole, levamisole, and their combination.the efficacy of the drugs flubendazole, levamisole, and their combination was studied against angiostrongylus cantonensis infection in mice. the drugs were evaluated on day 15 post infection. the efficacy was assessed by using single-dose and 5-dose treatment schedules. flubendazole was effective at 25 mg/kg, 50 mg/kg, 100 mg/kg, 200 mg/kg when given each day for five days. levamisole at a dose rate of 200 mg/kg was completely effective in a single treatment. the combined therapy of levamisole ( ...19873583131
1h- and 13c-nmr spectroscopic study of glucose metabolism in eggs of angiostrongylus cantonensis during their development.1h- and 13c-nuclear magnetic resonance (nmr) spectroscopy was used to study aerobic glucose metabolism in eggs of angiostrongylus cantonensis in an nctc-109 medium supplemented with fetal calf serum. without any pretreatment of the spent medium, we were able to identify and quantitate, by nmr, the end-products of glucose metabolism in eggs after cultivation for 2, 4, and 8 days. we demonstrated that a. cantonensis eggs took up glucose rapidly; among the major end products were found lactic acid, ...19873626133
angiostrongylus cantonensis: immunoblot analysis of the antigens recognized by rats.following infection of rats with angiostrongylus cantonensis, occurrence of anti-parasite antibody in the serum was determined with special reference to the antigens recognized by host igg antibodies, using sds-page combined with an immunoblotting technique. three saline extracts of digestive organ, reproductive organ and body wall, isolated from adult female a. cantonensis, were used as crude antigenic solutions. then 7 to 49 days after infection, igg antibodies directed predominantly against a ...19873112770
[immunologic findings in meningoencephalitis caused by neisseria meningitidis b 15, echo 4, and angiostrongylus cantonensis]. 19873125574
public health importance of angiostrongylus cantonensis and its relatives.species of angiostrongylus are fairly common nematode parasites of a variety of small mammals, but some species - particularly a. cantonensis and a. costaricensis - are increasingly seen as human infections. the worms make use of a range of aquatic, amphibious or terrestrial gastropod molluscs as intermediate hosts, which become infected by ingestion of eggs shed in the faeces of infected mammals. porotenic hosts, including crustaceans, amphibians and reptiles, are known for most angiostrongylus ...198715462892
[study on intracranial migration of angiostrongylus cantonensis in mice]. 19883163009
ocular angiostrongyliasis in japan: a case report.a small motile worm was found in the posterior pole of the eye of a patient, producing a decrease in visual acuity. the patient had signs of meningitis and had cerebrospinal fluid eosinophilia. his serum was positive for antibodies to angiostrongylus cantonensis. without surgical intervention, the patient recovered and his visual acuity returned to normal.19883341516
ultrastructural and morphometric analyses of eosinophils from the cerebrospinal fluid of the mouse and guinea-pig infected with angiostrongylus cantonensis.guinea-pigs infected with angiostrongylus cantonensis developed pleocytosis and eosinophilia in the cerebrospinal fluid (csf) at day 12 post-infection (p.i.), showing a peak response at day 20 p.i., followed by a gradual reduction. ultrastructural observations on csf eosinophils from infected mice and guinea-pigs revealed various signs of eosinophil degranulation after day 14 p.i., suggesting the exocytosis of lysosomal material. morphometric analysis indicated that csf eosinophils after day 22 ...19883174173
[radiculomyeloencephalitis caused by angiostrongylus cantonensis in children].a case of radiculoencephalomyelitis caused by central nervous system involvement by angiostrongylus cantonensis is reported in a 14 month-old girl. evolution was spontaneously favourable, which is not usual in severe types. lesions concerned the nevraxe and especially the spinal cord and are due to the migration of the larvae, creating eosinophilic inflammatory reactions.19883219042
t-cell-dependent eosinophilia in the cerebrospinal fluid of the mouse infected with angiostrongylus cantonensis.eosinophilia of the cerebrospinal fluid (csf) in permissive (rats) and non-permissive (mice) hosts infected with angiostrongylus cantonensis, and the possible mechanism of the eosinophilia were studied. in three strains of thymic mice (icr, ddy and balb/c), the infection provoked a marked csf eosinophilia starting at around day 12, reaching a peak level at day 20 and maintaining significantly high levels until day 35. in contrast, in athymic nude mice of balb/c strain the infection failed to evo ...19883259688
eosinophilic meningitis and radiculomyelitis in thailand, caused by cns invasion of gnathostoma spinigerum and angiostrongylus cantonensis.during the 6 year period from january 1980 to december 1985 44 patients with infection of the central nervous system by gnathostoma spinigerum or angiostrongylus cantonensis were admitted to the division of neurology, ramathibodi hospital, bangkok, thailand. in 16 patients the diagnosis could be confirmed serologically by means of elisa techniques. in gnathostomiasis encephalitis, myelitis, radiculitis and subarachnoid haemorrhage formed the majority of clinical syndromes. intracerebral haematom ...19883351533
1h- and 13c-nmr spectroscopic study of the metabolites in young adult angiostrongylus cantonensis maintained in vitro.1h- and 13c-nuclear magnetic resonance (nmr) spectroscopy were used to study metabolites excreted by young adult angiostrongylus cantonensis maintained aerobically in the presence of d-[13c6]glucose. end-products of glucose metabolism identified and quantitated by means of 1h-nmr were lactate, acetate and alanine, in the molar ratio of 1:0.13:0.05 for males and 1:0.07:0.04 for females. 13c-nmr analyses proved that all the three products originated from the glucose present in the medium.19883386682
angiostrongylus cantonensis and eosinophilic meningitis. 19882904084
local antibody production and immune complex formation in rats experimentally infected with angiostrongylus cantonensis.the systemic humoral immune responses and tissue localization of worm-antigen, antibodies (igg), and complement (c3) were examined in rats experimentally infected with angiostrongylus cantonensis. while the worms remained in the subarachnoid space, it was infiltrated with plasma cells and lymphoid cells containing igm and igg. when the infiltration of these cells became more pronounced, the serum antibody titer began to increase. at the same time, deposits of igm, igg, and c3 were found in the g ...19883056056
[a case of eosinophilic meningitis due to angiostrongylus cantonensis]. 19883069945
[procurement and evaluation of antigens of excretion-secretion in adult females of angiostrongylus cantonensis]. 19883072606
larvicidal effect of albendazole against angiostrongylus cantonensis in mice.mice were infected with 50 third stage larvae of angiostrongylus cantonensis and treated orally with albendazole (zentel) in dosages of 5, 10, and 25 mg/kg/day begun 5, 10, or 15 days post-infection for 7, 14, or 21 consecutive days. the mice in each group were killed 3 days after cessation of treatment and the brains examined for parasites. worms were recovered from the brains of all mice treated for 7 days, but recoveries from treated mice were significantly lower than from controls. worm redu ...19883407839
the finding of angiostrongylus cantonensis in rats in new orleans.twenty of 94 (21.4%) rattus norvegicus trapped in new orleans, louisiana, between april 1986 and february 1987 were infected with angiostrongylus cantonensis (3-62 worms per rat). this is the first report of the parasite from north america. a carnivorous snail, euglandina rosea, was found experimentally to be able to serve as both an intermediate and a paratenic host. other locally occurring gastropods that were successfully infected experimentally included mesodon thyroidus, anguispira alternat ...19883275136
immunoblot analysis of the circulating antigens occurring in serum of rats infected with angiostrongylus cantonensis.following the infection of rats with angiostrongylus cantonensis, the occurrence and molecular features of circulating antigens (cas) were analyzed, with special reference to their origin in worms, by sds-page combined with an immunoblotting technique. antisera against the cas were obtained by immunizing rats with sera from rats 35, 91, and 150 days postinfection. the anti-sera, referred to as anti-ca(35), anti-ca(91), and anti-ca(150), respectively, formed one or two precipitin lines when teste ...19883137559
preparation of monoclonal antibody against angiostrongylus cantonensis antigen.monoclonal antibodies (mab) against a cantonensis were produced through fusion of immunised spleen cells from balb/c mice with ns-1 myeloma cells at a ratio of 10:1. the successful fusion rate on the 3rd day of fusion was 90.1%. ten mab were characterised, six of which were igg1 and the remaining four were igg2a, igg2b, igm and iga respectively. among 6 igg1 mab, four were a. cantonensis-specific, of which three reacted to adult worm antigen only and one reacted to both adult worm and juvenile w ...19892772697
acquired immunity in rats against angiostrongylus cantonensis infection.acquired immunity against angiostrongylus cantonensis was induced by immunizing rats with somatic antigens from fifth-stage larvae and adult worms and live third-stage larvae. rats immunized twice had significantly fewer worms than rats immunized three times. fewer worms were recovered from rats immunized with 200 live third-stage larvae than from any other groups. rats immunized with somatic antigens had higher enzyme-linked immunosorbent assay (elisa) antibody levels than rats immunized with l ...19892807717
angiostrongylus cantonensis: role of eosinophils in the neurotoxic syndrome (gordon-like phenomenon).the role of eosinophils in the pathophysiology of angiostrongylus cantonensis infections was investigated in nonpermissive (guinea pig) and permissive (rat) hosts. neurological symptoms similar to the gordon phenomenon (ataxia, tremor, paralysis) together with a loss of purkinje cells in the cerebellum were observed after intracraneal injection of human eosinophil extracts or after infection with a. cantonensis, only in guinea pigs and not in rats. blood eosinophilia as well as eosinophil number ...19892721633
13c-nmr spectroscopic studies on the glucose metabolism of angiostrongylus cantonesis' eggs with special reference to the end-products and metabolic pathway.13c-nuclear magnetic resonance (nmr) spectroscopy was used to identify metabolites excreted by angiostrongylus cantonensis eggs which had been maintained aerobically in the presence of d-[13c6] glucose. using 13c-nmr we proved that lactate, acetate and alanine originated from glucose present in the medium via glycolysis. aminooxyacetate, an inhibitor of alanine transferase, inhibited simultaneously alanine production and the ability to take up glutamate, aspartate and valine from the medium. in ...19892608732
protective immunity against angiostrongylus cantonensis in rats following sensitizing infections.three strains (aci, august and wistar) of rats previously sensitized by oral infection with intact third-stage larvae of angiostrongylus cantonensis developed significant protective immunity to challenge infections 6 weeks later. the degree of the immunity was highest in the august strain of rats, followed by wistar and aci rats. protective immunity appears to affect both third-stage larvae and fourth- and/or fifth-stage worms. aci rats showed poor antibody responses, especially in the ige fract ...19892622895
characterization of the antigens that induce the production of antibodies in mice immediately after infection with angiostrongylus cantonensis.following the infection of mice (a non-permissive host) with angiostrongylus cantonensis, the occurrence of parasite-specific antibodies in the serum was monitored during the first 5 weeks after infection by the ouchterlony method and by an immunoblotting technique, with special reference to the antigens recognized by host igg antibodies. only the serum from infected mice 7 days postinfection formed a single, distinct precipitin line when tested on ouchterlony plates against extracts of digestiv ...19892671989
total and specific ige in serum and cerebrospinal fluid of rats and guinea pigs infected with angiostrongylus cantonensis.the total and specific ige response to angiostrongylus cantonensis infection was evaluated according to host permissiveness. total ige levels measured by a double antibody radioimmunoassay (ria) increased slowly in the permissive host (the rat), reaching a maximum between 4 and 8 weeks after infection. this maximum was earlier but significantly lower in the non-permissive host (the guinea pig). ige antibodies specific for adult worms or l1 or l3 larvae of a. cantonensis were measured by a radioa ...19892755934
in vivo efficacy of levamisole against larval stages of angiostrongylus cantonensis and a. costaricensis.anti-larval effects of levamisole were examined on a. cantonensis in rats and a. costaricensis in mice. 1) in rats inoculated with 40 infective larvae of a. cantonensis: compared with a non-treated control group, a significant reduction in number of worms recovered was seen in the group receiving a single dose of 1.0 mg/kg or more. a significant decrease in host lung-body weight ratio was seen in the group receiving drug of 3.0 mg/kg or more. 2) in mice inoculated with 20 infective larvae of a. ...19892772696
[scanning electron microscopic observations on adults of angiostrongylus cantonensis].scanning electron microscopic observations were made on adult angiostrongylus cantonensis, the apex of the head has a round mouth with two rows of 6 sensory papillae each around it. the papillae of the outer row are more rudimentary and inconspicuous. a pair of amphidial pores are visible on the external side of lateral papillae of the inner row. the surface of the body is covered with a circular striated cuticle and short longitudinal ridges lying between two circular cuticles. the two copulato ...19892766495
[intrathecal synthesis of immunoglobulins in meningoencephalitis caused by angiostrongylus cantonensis (chen, 1935)].four cases of eosinophil meningoencephalitis caused by angiostrongylus cantonensis are studied. the cytochemical study of the cerebrospinal fluid of these patients found a prevalence of eosinophils with increased ige. serum showed a variable behavior of immunoglobulins except for the increase of total ige. all patients showed igg and iga synthesis and there was igm synthesis in one patient. the percentage of intrathecal synthesis was variable and ige synthesis manifests in two trends with an equ ...19892486220
[scanning electron microscopic observations on larvae and young adults of angiostrongylus cantonensis].scanning electron microscopic observations on the structure of the body surface of various larval stages and young adults of angiostrongylus cantonensis were made. the mouth opening of the first and second stage larvae closes in "y" form until well developed young adult stage. there are two rows of 6 sensory papillae each around the mouth. with development of the worm, the papillae of the outer row gradually degenerated and could hardly be seen in adult worms. a pair of amphidial pores was prese ...19902099261
eosinophilic meningitis--a case report and literature review.a case of eosinophilic meningitis in a young boy from indonesia is reported. clinically, he had a subacute meningitis with eosinophilic pleocytosis in the cerebrospinal fluid. the clinical and laboratory findings strongly suggest angiostrongylus cantonensis as the aetiologic agent although the parasite was not found. the other most likely causes in the region are also considered and the life-cycle of angiostrongylus cantonensis and the epidemiology of its infection are briefly reviewed.19902133754
immunoglobulin g-dependent classical complement pathway activation in neutrophil-mediated cytotoxicity to infective larvae of angiostrongylus cantonensis.the participation of antibody and complement in cell-mediated adherence and cytotoxicity to infected larvae (l3) of angiostrongylus cantonensis was investigated in vitro. of the different cell types involved in the reaction, neutrophils were seen to have a predominant role in immune serum--dependent adherence and cytotoxicity to l3. in the presence of immune serum, cytotoxicity to l3 by neutrophils from infected rats was twice that of neutrophils from normal rats. although mononuclear cells and ...19902200361
alteration in density of eosinophils in the cerebrospinal fluid of mice infected with angiostrongylus cantonensis.the density of eosinophils in the cerebrospinal fluid (csf) and blood of male ddy mice infected with angiostrongylus cantonensis was examined on days 14, 20 and 27 post-infection (p.i.) with discontinuous percoll gradient centrifugation. normal blood eosinophils had a density of between 1.070 and 1.080 g ml-1. no significant changes in density in blood eosinophils were noted during the course of the observations. csf eosinophils began to become hypodense (defined as density less than 1.070 g ml- ...19902228429
effects of infection with angiostrongylus cantonensis on the circulating haemocyte population and the haematopoietic organ of the host snail m-line biomphalaria glabrata.the number of circulating haemocytes, the size of the haematopoietic organ, and the size of haemocyte capsules around the parasite were studied in m-line biomphalaria glabrata snails exposed to 100 or 400 first-stage larvae of angiostrongylus cantonensis. the number of haemocytes in exposed snails increased significantly at 1 day post-exposure, decreased to control value, and then increased again. the decrease in number of circulating haemocytes is probably due to the removal of cells from the c ...19902230034
a survey of ampullarium canaliculatus for natural infection of angiostrongylus cantonensis in south taiwan.ampullarium canaliculatus were collected monthly from the field in five areas in pintung county and examined for larvae of angiostrongylus cantonensis. snails from one area showed the highest positive rate (30.8%) and the highest number (31) of larvae per snail. snails were placed in four groups according to size. the positive rates in snails increased from 10.0 to 29.4% and the average number of larvae per snail increased from 11 to 40 as the size of snails increased from 1.0 to 4.0 cm height o ...19902231842
eosinophilic meningitis: cause of a chronic pain syndrome.three tourists developed eosinophilic meningitis after visiting the fijian islands. two had a severe and long lasting illness with chronic intractable pain. in one patient electrophysiological studies and mri scan of the brain were abnormal and provided evidence of both radicular and cerebral parenchymal involvement by the most likely causative agent, angiostrongylus cantonensis.19902246659
eosinophilic meningoencephalitis due to angiostrongylus cantonensis as the cause of death in captive non-human primates.fatal eosinophilic meningoencephalitis due to angiostrongylus cantonensis is reported in captive non-human primates. a howler monkey (alouatta caraya) at the audubon park and zoological gardens, new orleans, la, died 21 days after initial clinical symptoms. a white-handed gibbon (hylobates lar) died at the ardastra gardens and zoo, nassau, bahamas, 17 days after onset of symptoms. both had access to free-ranging gastropods within the zoos. these are the first reported cases of natural infection ...19902301708
application of 15n-nuclear magnetic resonance (n.m.r.) spectroscopy for the study of nitrogen metabolism of a parasite: transamination in angiostrongylus cantonensis eggs.in an attempt to study nitrogen metabolism in a parasite, we applied 15n-nuclear magnetic resonance (nmr) technology with a stable isotope, a 15n-labeled compound, for the study of the transamination system in a. cantonensis eggs, and demonstrated that 15n-aspartic acid can serve as an amino group donor for both the 2-oxoglutaric-glutamic acid and the pyruvic acid-alanine transamination systems in the eggs.19902312222
angiostrongylus cantonensis: in vitro cultivation from the first-stage to infective third-stage larvae.the first-stage larvae of angiostrongylus cantonensis were cultured in various media at 27 degrees c. the most suitable medium for the development was chernin's balanced salt solution supplemented with 10% l-15, 10% tryptose phosphate broth, 20% fetal calf serum, and 26 mm sodium bicarbonate. addition of sodium bicarbonate to the medium facilitated early development of the first-stage larvae. when the first-stage larvae were cultured in the medium under 5% co2 in air, the worms developed gradual ...19902323398
eosinophilic meningitis caused by angiostrongylus cantonensis. 19902367166
eosinophilic meningitis in thailand. clinical and epidemiological characteristics of 162 patients with myeloencephalitis probably caused by gnathostoma spinigerum.the clinical and epidemiological characteristics of 162 patients with eosinophilic myeloencephalitis, believed to be caused by invasion of the central nervous system by gnathostoma spinigerum, are described. the case mortality rate was at least 12%, and probably higher. single live young adult g. spinigerum were recovered from the brains of 2 patients at autopsy and from the eye lids of 2 other living patients. the disease caused by this parasite can be distinguished from that caused by angiostr ...19902376755
lipids of angiostrongylus cantonensis (nematoda: metastrongyloidea): a comparison between young adults and gravid worms.1. all major classes of lipids were found in the young adults in brain (22 days post-infection) and gravid angiostrongylus cantonensis in lung of rats (34 days post-infection) comprising approximately 60% of phospholipids, 30% of neutral lipids and the rest, glycolipids. 2. the relative composition of phospholipids were quite similar between worms from the two different habitats, with phosphatidylcholine predominating. the glycolipid profiles were also similar. 3. more neutral lipids in the worm ...19902331872
differences in responses of rat- and guinea-pig-eosinophils to eosinophil chemotactic factors derived from angiostrongylus cantonensis.eosinophil chemotactic activity associated with whole worm extracts of the young adult worms (ya) and 1st stage larvae (l1) of angiostrongylus cantonensis was assessed using guinea-pig- and rat-eosinophils. both whole worm extracts were potently chemotactic to guinea-pig-eosinophils whereas only the whole worm extract of l1 was chemotactic to rat-eosinophils. gel filtration chromatography of ya-whole worm extract yielded an eosinophil chemotactic factor (ecf-ya) with an estimated molecular weigh ...19902385443
effect of flubendazole on the number of first-stage larvae of angiostrongylus cantonensis released in the faeces of treated rats.the effect of flubendazole orally administered at 10 mg/kg/day for 5 consecutive days (the 11th, 20th or 40th post-infection) on the number of first-stage larvae (l1) of angiostrongylus cantonensis released in the faeces of rats each infected with 40 third-stage larvae was determined. faecal examination for 5 months, the period from medication to dissection of rats, showed that l1 release ceased in all the rats of medicated groups by about 1 week after the termination of dosing and resumed 1-2 m ...19902387978
characterization of monoclonal antibodies to young-adult worms of angiostrongylus cantonensis.three igg1 and one igg2a monoclonal antibodies were obtained by fusion of ns-1 cells with spleen cells of balb/c mice immunized with young-adult worm antigen of a. cantonensis. these 4 monoclonal antibodies were specific to a. cantonensis without cross-reactivity to any antigens of toxocara canis, ascaris suum, clonorchis sinensis, dirofilaria immitis, paragonimus westermani, gnathostoma spinigerum, strongyloides stercoralis and anisakis spp. detected by elisa. the affinity constants of monoclon ...19901701759
Refresh
firstprev
12345678910
nextlast
Displaying items 201 - 300 of 971