Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| theratromyxa weberi, an amoeba predatory on plant-parasitic nematodes. | theratromyxa weberi, an amoeba, was isolated and cultured in the laboratory to determine the physical conditions that influence its predation on plant parasitic nematodes. in greenhouse tests, the amoeba was a poor biological control agent of the root-knot nematode, meloidogyne incognita. | 1973 | 19319347 |
| nematodes attacking cultivars of peach in north carolina. | criconemoides xenoplax and meloidogyne incognita were the nematode species most frequently associated with peach in north carolina. other nematodes often found in high numbers on that crop were pratylenehus vulnus, helicotylenchus spp., trichodorus christiei, xiphinema amerieanum and tylenchorhynchus claytoni. p. vulnus and p. penetrans reproduced well on rootstocks of 21 peach cultivars tested in the greenhouse. p. zeae, p. brachyurus, p. coffeae and p. scribneri decreased or increased only sli ... | 1973 | 19319348 |
| fine structure of cephalic sense organs in meloidogyne incognita males. | amphids, and the cephalic and labial papillae of meloidogyne incognita males were examined in detail by electron microscopy. each amphid basically consists of an amphidial gland, a nerve bundle and an amphidial duct. the gland is a broad microvillous organ with a narrow anterior process, which is closely associated with the amphidial duct. a posterior process of the gland contains secretory organelles and proceeds along the esophagus with the lateral cephalic nerve bundle. the nerve bundle penet ... | 1973 | 19319352 |
| response of soybean cultivars to nematicidal treatments of soil infested with meloidogyne incognita. | a comparison of untreated and nematicide-treated soil for soybean production revealed that meloidogyne incognita hastened crop maturity and reduced plant ht, seed wt, and yield. reductions of yield varied from 32-90% depending on cultivar susceptibility. dbcp was more consistent in increasing crop performance than organo-phosphale or oxime carbamate nematicides. greatest yield increases were produced by nematicidal treatment of soils planted to soybean cultivars with the lowest susceptibility. | 1974 | 19319356 |
| resistance of cotton to the root-knot nematode, meloidogyne incognita. | cotton plants resistant to meloidogyne incognita had roots characterized by fewer and smaller galls, and females that produced fewer egg masses containing fewer eggs than did susceptible plants. many galls on resistant roots contained no nematodes at the time of examination. penetration of the resistant cultivar was equal to that of the susceptible cultivar and independent of the number of nematodes in the inoculum. fewer nematodes penetrated resistant or susceptible plants with eight leaves tha ... | 1974 | 19319358 |
| post-infection development and histopathology of meloidogyne incognita in resistant cotton. | the numbers of meloidogyne incognita larvae which migrated from cotton roots declined over a 16-day period, but the difference in numbers migrating from resistant and susceptible cultivars was not significant. larvae penetrated susceptible roots, matured, and reproduced within 14 days following inoculation, whereas nematode development in the resistant roots was greatly retarded. three types of histological responses were observed in infected, resistant roots, and these correlated with the degre ... | 1974 | 19319359 |
| the role of secondary invaders in meloidogyne incognita infection. | secondary microbial invasion of tomato plants inoculated with 6,000 meloidogyne incognita larvae caused 75 and 48% weight reduction of foliage and roots, respectively. under aseptic conditions the same number of larvae caused only 37% reduction of foliage and increased root weight by 50%. | 1970 | 19322275 |
| symptomless resistance of alfalfa to meloidogyne incognita acrita. | penetration, development and migration of the cotton root-knot nematode, meloidogyne incognita acrita, in resistant and susceptible alfalfa varieties was compared. larvae entered both resistant and susceptible plants in approximately the same numbers. after 3 to 4 days, the number of larvae in resistant roots decreased sharply until at 7 days fewer than 5 larvae/seedling and no nematode development could be found. in susceptible roots, larvae became sedentary and developed normally; egg producti ... | 1970 | 19322284 |
| resistance of cucumis spp. to the root-knot nematode, meloidogyne incognita acrita. | the nature of resistance in cucumis ficifolius and c. metuliferus to the root-knot nematode, meloidogyne incognita acrita, was studied under greenhouse conditions. although as many larvae penetrated the roots of these species as those of the susceptible c. melo, few developed to the adult female stage. resistance in c. ficifolius and c. metuliferus was associated with hindrance of larval development beyond the second stage, delayed development of larvae to adults and stimulation toward maleness. ... | 1970 | 19322292 |
| pathogenicity of pythium aphanidermatum to chrysanthemum in combined inoculations with belonolaimus longicaudatus or meloidogyne incognita. | rooted cuttings of 'iceberg' chrysanthemum in steamed soil were inoculated with the nematodes belonolaimus longicaudatus, and meloidogyne incognita, alone and combined with pythium aphanidermatum, a fungus pathogen of chrysanthemum. b. longicaudatus alone severely restricted the root system; with p. aphanidermatum also present, plant weight and height were further reduced and onset of symptoms was earlier. m. incognita + fungus interaction was similar but less intense. the fungus suppressed egg ... | 1970 | 19322307 |
| hatching response of meloidogyne incognita acrita to electric shock. | the influence of electric shock on hatch of meloidogyne incognita acrita from egg masses taken from roots of 'acala sj-i' cotton (gossypium hirsutum l.) was studied. egg masses in tap water were individually placed between the tips of needle electrodes 1 mm apart and exposed to potentials of l, 10, 20, and 60 vdc/mm at 1, 1, 1, and 86 milliamperes dc, respectively, for periods of 2 and 60 seconds. hatched larvae were counted at five-day intervals for 60 days. of the eight treatment combinations ... | 1970 | 19322315 |
| nematode electrocution. | the effects of electric shock on panagrellus redivivus adults and larvae and meloidogyne incognita acrita larvae were studied. the nematodes were placed in tap water between two stainless steel electrodes, spaced 2 mm apart and cemented to a glass slide. electric potentials of 1, 5, 10, 15, 20, 30, and 60 vdc/mm and vac/mm were applied for periods of 1 sec to 5 rain at 0.05 to 77 ma. the results demonstrated that ac or dc electric shocks as low as 5 v/mm for larvae and 10 v/mm for adults can be ... | 1970 | 19322316 |
| interactions between meloidogyne incognita, m. hapla, and pratylenchus brachyurus in tobacco. | in a greenhouse pot experiment on the pathogenicity and interactions of meloidogyne incognita, m. hapla and pratylenchus brachyurus on four cultivars o f tobacco the cultivars 'hicks' and 'nc 2326' were susceptible to each nematode and "nc 95' and 'nc 2512' resistant only to m. incognita.mean heights of susceptible plants were depressed but fresh weight of tops did not differ significantly. meloidogyne spp. increased fresh weight of susceptible (but not the resistant) roots.reproduction of m. in ... | 1970 | 19322321 |
| meloidogyne incognita and production of leaf epicuticular wax in cotton. | 1970 | 19322333 | |
| the rhizoctonia-meloidogyne disease complex in flue-cured tobacco. | if meloidogyne incognita preceded rhizoctonia solani by 10 days or 21 days in roots of greenhouse-grown tobacco plants, root rot was more extensive than when the nematode and fungus were introduced either simultaneously or separately or when r. solani was added after artificial wounding. histological examination of galled roots 72 days after inoculation with r. solani revealed extensive fungal colonization in the root-knot susceptible cultivar 'dixie bright 101' when m. incognita preceded r. sol ... | 1971 | 19322362 |
| differential vertical movement of nonvolatile nematicides in soil. | when incorporated in the top 5-cm of tifton sandy loam at 11.2 kg/ha in the field, b-68138 [ethyl 4-(methylthio)-m-tolyl isopropyl phosphoramidate] prevented galling of tomato roots by meloidogyne sp. down to 20 cm. a similar application of 16.8 kg/ha of v-c 9-104 [0-ethyl s,s-dipropyl phosphorodithioate] was 99% effective down to 20 cm. aldicarb [2-methyl-2-(methylthio) propionaldehyde 0-(methylcarbamoyl) oxime], b-25141 [0,0-diethyl 0-{p-(methylsulfinyl) phenyl}phosphorothioate], and carbofura ... | 1971 | 19322385 |
| reaction of field-grown sericea lespedeza to selected meloidogyne spp. | five sericea lespedeza (lespedeza cuneata [dumont] g. don) breeding lines resistant to meloidogyne incognita, m. incognita acrita, and m. hapla as seedlings in greenhouse tests and two varieties were resistant to m. incognita acrita in field experiments. root-knot galling and larvae numbers were less for resistant entries than for the susceptible check when grown in root-knot infested field soil for three growing seasons. forage yields were as much as 57 times greater for resistant entries than ... | 1971 | 19322393 |
| the hypersensitivity reaction of tomatoes resistant to meloidogyne incognita: reversal by cytokinins. | initiation of larval growth, induction of cell necrosis, and gall formation in the host were measured as criteria of resistance or susceptibility of tomato seedlings to the root-knot nematode, meloidogyne incognita (kofoid &white) chitwood. seedlings grown at 27 c on water agar containing additions were scored 3 or 4 days after infection.in the absence of exogenous plant growth regulatory substances, approximately 73% of larvae that entered roots of susceptible plants showed growth, none induced ... | 1969 | 19325655 |
| effect of meloidogyne incognita, m. hapla, and m. javanica on the severity of fusarium wilt of chrysanthemum. | rooted cuttings of chrysanthemum morifolium 'yellow delaware' (fusarium-susceptible) and 'white iceberg' (fusarium-resistant) were greenhouse-grown in: (i) non-infested soil; (ii) soil infested with fusarium oxysporum alone; (iii) soil infested with meloidogyne incognita, m. javanica or m. hapla; and (iv) each nematode separately plus the fungus. all nematode species infected roots of both cultivars and caused characteristic root-knot symptoms but did not appreciably affect growth meassured by p ... | 1969 | 19325666 |
| the relationship of meloidogyne incognita acrita to the incidence of cabbage yellows. | three inoculum level combinations of meloidogyne incognita acrita and fusariurn oxysporurn f. conglutinans did not affect the incidence of cabbage yellows in the moderately-resistant 'greenback' or the highly-resistant 'marion market' varieties. the susceptible 'early round dutch' variety was more susceptible to low levels of fungus inoculum in the presence of the nematode. | 1969 | 19325680 |
| effects of storage temperature and extraction procedure on recovery of plant-parasitic nematodes from field soils. | storage of nematodes in soil at -15 c for 1 to 16 weeks greatly increased nematode recovery by a sugar-flotation-sieving procedure. one week of exposure to -15 c killed all nematodes except pratylenchus zeae and tylenchorhynchus claytoni which were recoverable in decreasing numbers up to 10 weeks by the baermann funnel method. optimum storage temperature for survival of most nematode species was 13 c. the numbers of meloidogyne incognita, t. claytoni, belonolaimus iongicaudatus, and p. zeae reco ... | 1969 | 19325684 |
| the histochemical localization of several enzymes of soybeans infected with the root-knot nematode meloidogyne incognita acrita. | the sites of activity of alkaline phosphatase, acid phosphatase, esterase, peroxidase, adenosine triphosphatase, and cytochrome oxidase were demonstrated histochemically in fresh sections of 'lee' soybeans infected by the root-knot nematode meloidogyne incognita acrita. each of the six enzymes was more active at the sites of infection than in adjacent non-infected tissue. during the early stages of infection, an increase in enzyme activity was observed in several cells in the proximity of the li ... | 1969 | 19325688 |
| the genomes of root-knot nematodes. | plant-parasitic nematodes are the most destructive group of plant pathogens worldwide and are extremely challenging to control. the recent completion of two root-knot nematode genomes opens the way for a comparative genomics approach to elucidate the success of these parasites. sequencing revealed that meloidogyne hapla, a diploid that reproduces by facultative, meiotic parthenogenesis, encodes approximately 14,200 genes in a compact, 54 mpb genome. indeed, this is the smallest metazoan genome c ... | 2009 | 19400640 |
| characterization of a putative endoxylanase in the migratory plant-parasitic nematode radopholus similis. | plant-parasitic nematodes have developed an arsenal of enzymes to degrade the rigid plant cell wall. in this article, we report the presence of a putative endoxylanase in the migratory endoparasitic nematode radopholus similis. this enzyme is thought to facilitate the migration of the nematode, as it breaks down xylan, the major component of hemicellulose. the corresponding gene (rs-xyl1) was cloned and the sequence revealed three small introns. interestingly, the position of all three introns w ... | 2009 | 19400841 |
| biocontrol of meloidogyne incognita on tomato using antagonistic fungi, plant-growth-promoting rhizobacteria and cattle manure. | biocontrol achieved by a single biocontrol agent is generally inconsistent under field conditions. the aim of the present study was to increase the competitiveness and efficacy of biocontrol agents by using them together with cattle manure. | 2009 | 19431151 |
| spread of rotylenchulus reniformis in an arkansas cotton field over a four-year period. | rotylenchulus reniformis was first detected in a single grid (100 m(2)) in may 2001 in a cotton field in ashley county, ar, that was being utilized to evaluate the utility of grid-sampling for detection of meloidogyne incognita. a total of 512 grids were sampled in the 6-ha field in the spring and fall for four years (2001 - 2004), nematode populations were determined for each grid, and nematode population density maps were constructed utilizing global positioning systems and geographic informat ... | 2008 | 19440255 |
| alternate row placement is ineffective for cultural control of meloidogyne incognita in cotton. | the objective of this study was to determine if planting cotton into the space between the previous year's rows reduces crop loss due to meloidogyne incognita compared to planting in the same row every year. row placement had a significant (p </= 0.05) effect on nematode population levels only on 8 july 2005. plots receiving 1,3-dichloropropene plus aldicarb had lower nematode population levels than non-fumigated plots on 24 may and 8 july in 2005, but not in 2004. the effect of nematicide treat ... | 2008 | 19440259 |
| european corn borer (lepidoptera: crambidae) stalk tunneling on root-knot nematode (tylenchida: heteroderidae) fitness on corn. | greenhouse experiments were conducted in 2004-2006 to examine the reciprocal effects of aboveground herbivory by european corn borer, ostrinia nubilalis (hübner) (lepidoptera: crambidae), and belowground herbivory by root-knot nematode, meloidogyne incognita chitwood (tylenchida: heteroderidae), on one another at three corn, zea mays l., growth stages. two experiments were conducted to study the effect of aboveground herbivory by o. nubilalis on the number of m. incognita juvenile penetration/ro ... | 2009 | 19449640 |
| plant growth-promoting rhizobacteria, paenibacillus polymyxa and paenibacillus lentimorbus suppress disease complex caused by root-knot nematode and fusarium wilt fungus. | to screen and evaluate the biocontrol potential of paenibacillus strains against disease complex caused by meloidogyne incognita and fusarium oxysporum f. sp. lycopersici interactions. | 2009 | 19457027 |
| non-nematode-derived double-stranded rnas induce profound phenotypic changes in meloidogyne incognita and globodera pallida infective juveniles. | nine non-nematode-derived double-stranded rnas (dsrnas), designed for use as controls in rna interference (rnai) screens of neuropeptide targets, were found to induce aberrant phenotypes and an unexpected inhibitory effect on motility of root knot nematode meloidogyne incognita j2s following 24h soaks in 0.1 mg/ml dsrna; a simple soaking procedure which we have found to elicit profound knockdown of neuronal targets in globodera pallida j2s. we have established that this inhibitory phenomenon is ... | 2009 | 19482028 |
| nematicidal activity of paecilomyces spp. and isolation of a novel active compound. | many species of paecilomyces are entomogenous fungi and several are efficacious toward nematodes. to study the potential of paecilomyces species in controlling nematodes, fungal extracts of 40 paecilomyces spp. were evaluated for their nematicidal activity against bursaphelenchus xylophilus and panagrellus redivivus. the extracts of six paecilomyces spp. exhibited the nematicidal activity against p. redivivus, and 11 species exhibited the nematicidal activity against b. xylophilus. the methanol ... | 2009 | 19557340 |
| virulence development and genetic polymorphism in meloidogyne incognita (kofoid & white) chitwood after prolonged exposure to sublethal concentrations of nematicides and continuous growing of resistant tomato cultivars. | the root-knot nematode, meloidogyne incognita (kofoid & white) chitwood, is an important plant pathogen damaging to tomato. continuous use of resistant tomato cultivars and nematicides for its effective management might lead to resistance break-up or nematicide failure. genetic variability and virulence in m. incognita on susceptible pusa ruby tomato were analysed by bioassay, esterase and dna polymorphism after a 5 year weekly exposure to carbofuran, carbosulfan, cadusafos and triazophos at 0.0 ... | 2009 | 19562824 |
| phenotypic and molecular evaluation of cotton hairy roots as a model system for studying nematode resistance. | agrobacterium rhizogenes-induced cotton (gossypium hirsutum l.) hairy roots were evaluated as a model system for studying molecular cotton-nematode interactions. hairy root cultures were developed from the root-knot nematode (rkn) (meloidogyne incognita [kofoid and white] chitwood, race 3)-resistant breeding line m315 and from the reniform nematode (rn) (rotylenchulus reniformis linford & oliveira)-resistant accession gb713 (g. barbadense l.) and compared to a nematode-susceptible culture derive ... | 2009 | 19578854 |
| short interfering rna-mediated gene silencing in globodera pallida and meloidogyne incognita infective stage juveniles. | the analysis of gene function through rna interference (rnai)-based reverse genetics in plant parasitic nematodes (ppns) remains inexplicably reliant on the use of long double-stranded rna (dsrna) silencing triggers; a practice inherently disadvantageous due to the introduction of superfluous dsrna sequence, increasing chances of aberrant or off-target gene silencing through interactions between nascent short interfering rnas (sirnas) and non-cognate mrna targets. recently, we have shown that no ... | 2010 | 19651131 |
| characterization and gene cloning of a novel serine protease with nematicidal activity from trichoderma pseudokoningii smf2. | trichoderma pseudokoningii smf2 is a biocontrol fungus with inhibitory ability against phytopathogenic fungi. here, a crude extract of strain smf2 in a solid ferment exhibited strong nematicidal activity against meloidogyne incognita, and a novel serine protease sprt with nematicidal activity was purified from the crude extract. protease sprt has a molecular mass of 31 kda, a ph optimum of 8.5, and a temperature optimum of 60-65 degrees c. it had good thermostability, and was stable in an alkali ... | 2009 | 19702879 |
| modular architecture and evolution of the map-1 gene family in the root-knot nematode meloidogyne incognita. | in eukaryotes, repeat proteins (i.e. proteins that contain a tandem arrangement of repeated structural elements) are often considered as an extra source of variability, and gains and losses of repeats may be an important force driving the evolution and diversification of such proteins, that could allow fast adaptation to new environments. here, we report genomic sequences of the map-1 protein family from of the asexual, plant-parasitic nematode meloidogyne incognita. the encoded proteins exhibit ... | 2009 | 19787376 |
| actin-depolymerizing factor2-mediated actin dynamics are essential for root-knot nematode infection of arabidopsis. | reorganization of the actin and microtubule networks is known to occur in targeted vascular parenchymal root cells upon infection with the nematode meloidogyne incognita. here, we show that actin-depolymerizing factor (adf) is upregulated in the giant feeding cells of arabidopsis thaliana that develop upon nematode infection and that knockdown of a specific adf isotype inhibits nematode proliferation. analysis of the levels of transcript and the localization of seven adf genes shows that five ar ... | 2009 | 19794115 |
| fmrfamide-like peptides in root knot nematodes and their potential role in nematode physiology. | fmrfamide-like peptides (flps) are a diverse group of neuropeptides that are expressed abundantly in nematodes. they exert potent physiological effects on locomotory, feeding and reproductive musculature and also act as neuromodulators. however, little is known about the specific expression patterns and functions of individual peptides. the current study employed rapid amplification of cdna ends-polymerase chain reaction (race-pcr) to characterize flp genes from infective juveniles of the root k ... | 2010 | 19843350 |
| [steroid furostanol glycosides: a new class of natural adaptogenes (review)]. | the present review summarizes experimental data revealed while studying the mechanism of the adaptogenic effect of furostanol glycosides (fg) extracted from dioscorea deltoidea wall cell culture under the conditions of biotic stress in tomato plants lycopersicon esculenium mill. induced by the gall nematode meloidogyne incognita kofoid et white. comparison of changes in isoprene content (phytosterines, tomatin, and carotenoids) and in the rate of oxidative processes in the leaves and roots of in ... | 2009 | 19845281 |
| development and evaluation of robust molecular markers linked to disease resistance in tomato for distinctness, uniformity and stability testing. | molecular markers linked to phenotypically important traits are of great interest especially when traits are difficult and/or costly to be observed. in tomato where a strong focus on resistance breeding has led to the introgression of several resistance genes, resistance traits have become important characteristics in distinctness, uniformity and stability (dus) testing for plant breeders rights (pbr) applications. evaluation of disease traits in biological assays is not always straightforward b ... | 2010 | 19855951 |
| jasmonic acid and tomato resistance to the root-knot nematode meloidogyne incognita. | 2016 | 19994787 | |
| nematodetoxic aurovertin-type metabolites from a root-knot nematode parasitic fungus pochonia chlamydosporia. | chemical investigation of one fungal strain p. chlamydosporia ymf 1.00613 isolated from root knots of tobacco infected by meloidogyne incognita led to the isolation and identification of four aurovertin-type metabolites, which include a new compound, aurovertin i (a1), and three known metabolites, aurovertins e, f and d (a2-a4). their structures were established by spectroscopic studies such as 1d- and 2d-nmr and ms analysis. aurovertin i (a1) is the first natural product with an aurovertin skel ... | 2010 | 20000774 |
| heterologous expression of taro cystatin protects transgenic tomato against meloidogyne incognita infection by means of interfering sex determination and suppressing gall formation. | plant-parasitic nematodes are a major pest of many plant species and cause global economic loss. a phytocystatin gene, colocasia esculenta cysteine proteinase inhibitor (cecpi), isolated from a local taro kaosiang no. 1, and driven by a camv35s promoter was delivered into cln2468d, a heat-tolerant cultivar of tomato (solanum lycopersicum). when infected with meloidogyne incognita, one of root-knot nematode (rkn) species, transgenic t1 lines overexpressing cecpi suppressed gall formation as evide ... | 2010 | 20054551 |
| nematicidal activity of monoterpenoids against the root-knot nematode meloidogyne incognita. | abstract nematicidal activity of 22 monoterpenoids were evaluated in vitro and in pot experiments. twenty of the twenty-two monoterpenoids significantly reduced hatching, and 11 reduced j2 mobility of the root-knot nematode meloidogyne incognita at a concentration of 250 mg/liter. in general, compounds with hydroxyl and carbonyl groups exhibited higher nematicidal activity than other terpenoids. borneol, carveol, citral, geraniol, and alpha-terpineol showed the highest nematicidal activity among ... | 2010 | 20055654 |
| persistence and nematicidal efficacy of carbosulfan, cadusafos, phorate, and triazophos in soil and uptake by chickpea and tomato crops under tropical conditions. | the productivity of chickpea, cicer arietinum (l.), and tomato, solanum lycopersicum (l.), is adversely affected by root-knot nematode, meloidogyne species. nematode-resistant chickpea and tomato are lacking except for a few varieties and therefore grower demand is not met. the available nematicides, namely, carbosulfan, cadusafos, phorate, and triazophos, were, therefore evaluated for their efficacy and persistence in soil and crops to devise nematode management decisions. in alluvial soil, cad ... | 2010 | 20085277 |
| additive effects of plant expressed double-stranded rnas on root-knot nematode development. | ectopically expressed double-stranded rnas (dsrnas) have recently been shown to suppress parasitic success of meloidogyne spp. in plants. we have targeted two genes from the root-knot nematode meloidogyne incognita; a dual oxidase gene implicated in the tyrosine cross-linking of the developing cuticle and a subunit of signal peptidase, a protein complex required for the processing of secreted proteins. while these genes are involved in different aspects of nematode development, the phenotypic co ... | 2010 | 20100489 |
| microwave assisted solvent-free synthesis and biological activities of novel imines (schiff bases). | twelve new ortho-hydroxyketimines were synthesized by conventional as well as microwave method and evaluated for their antinemic activity against meloidogyne incognita [(kofoid and white) chitwood]. conventional methods for synthesis of schiff bases require refluxing at 140 degrees c of the reactants in different solvents for at least 24 h or more, where as the microwave-assisted synthesis has brought down the reaction time from 24 h to 1 minute. the procedure reported is simple as it does not r ... | 2009 | 20183046 |
| effects of a novel pathogenesis-related class 10 (pr-10) protein from crotalaria pallida roots with papain inhibitory activity against root-knot nematode meloidogyne incognita. | a novel pathogenesis-related class 10 (pr-10) protein with papain inhibitory activity, named cppri, was purified from crotalaria pallida roots by ammonium sulfate precipitation followed by three reverse-phase high-performance liquid chromatographies (hplcs). cppri is made up of a single polypeptide chain with a m(r) of 15 kda, as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page). this protein exhibited a k(i) value of 1.8 x 10(-9) m and operates via a noncompetit ... | 2010 | 20199085 |
| purification, characterization and molecular cloning of a monocot mannose-binding lectin from remusatia vivipara with nematicidal activity. | a mannose-binding lectin (rvl) was purified from the tubers of remusatia vivipara, a monocot plant by single-step affinity chromatography on asialofetuin-sepharose 4b. rvl agglutinated only rabbit erythrocytes and was inhibited by mucin, asialomucin, asialofetuin and thyroglobulin. lectin activity was stable up to 80 degrees c and under wide range of ph (2.0-9.3). sds-page and gel filtration results showed the lectin is a homotetramer of mr 49.5 kda, but maldi analysis showed two distinct peaks ... | 2010 | 20213246 |
| management of root-knot nematode, meloidogyne incognita in carrot. | the root-knot nematode, meloidogyne incognito, remains to be one of the most important constraints in agricultural production worldwide. however, reports showed that root-knot nematode (rkn) population can be suppressed by addition of organic amendments. a greenhouse microplot experiment was conducted to determine if locally available organic amendments would reduce rkn population and improve the growth and yield of more susceptible and less susceptible carrot cultivars in comparison with the fa ... | 2009 | 20222624 |
| nematicidal natural products from the aerial parts of rubus niveus. | studies on the aerial parts of rubus niveus yielded six known compounds, 3,5-dihydroxy benzoic acid c(7)h(6)o(4), (1), gallic acid c(7)h(6)o(5) (2), ethyl galactoside (3), oleanolic acid (4), beta-sitosterol (5) and 3-o-[beta-d-galactopyranosyl-(12)-d-glucopyranoside (6). besides this, a gallic acid derivative with methyl substitution was synthesised as tetramethyl gallate (3). together with this derivative, compounds 1, 2, the alcohol soluble, chloroform soluble and petroleum ether soluble extr ... | 2010 | 20306362 |
| in vitro comparison of protease activities in preparations from free-living (panagrellus redivivus) and plant-parasitic (meloidogyne incognita) nematodes using fmrfa and fmrfa-like peptides as substrates. | extracts prepared from the microbivorous nematode panagrellus redivivus and the plant-parasitic nematode meloidogyne incognita were used to provide general protease activities for peptide substrate screening and species comparisons. each extract was evaluated for its ability to degrade a broad range of nematode fmrfamide-like peptides (flps), key regulatory messengers governing nematode growth and development. clear quantitative differences between the two extracts were observed using fmrfamide ... | 2010 | 20334714 |
| biocatalytic amidation of carboxylic acids and their antinemic activity. | a series of novel n-alkyl substituted amides, synthesized by enzyme catalysis, were evaluated against root-knot nematode, meloidogyne incognita and found to have potential antinemic activity. the corresponding amides were prepared by the condensation of equimolar amounts of carboxylic acids with different alkyl amines in the presence of candida antarctica lipase at 60-90 degrees c in 16-20 h. the reactions were carried out in a non - solvent system without the use of any activating agents. all t ... | 2010 | 20390959 |
| short interfering rna-mediated knockdown of drosha and pasha in undifferentiated meloidogyne incognita eggs leads to irregular growth and embryonic lethality. | micro-(mi)rnas play a pivotal role in the developmental regulation of plants and animals. we reasoned that disruption of normal heterochronic activity in differentiating meloidogyne incognita eggs may lead to irregular development, lethality and by extension, represent a novel target for parasite control. on silencing the nuclear rnase iii enzyme drosha, a critical effector of mirna maturation in animals, we found a significant inhibition of normal development and hatching in short interfering ( ... | 2010 | 20398669 |
| nematicidal natural products from the aerial parts of buddleja crispa. | studies on the aerial parts of buddleja crispa yielded 13 known compounds, nonyl benzoate, hexyl p-hydroxy-cinnamate, ginipin, gardiol, 1-heptacosanol, steroidal galactoside (22 r)-stigmasta-7,9 (11)-dien-22 beta-ol-3beta-o-beta-d-galactopyranoside, 3-methoxy benzoic acid, beta-sitosterol and ursolic acid. besides this two iridoid galactosides buddlejosides a, buddlejosides b and a benzofuran-type sesquiterpene buddlejone have been isolated from the etoac fraction of b. crispa. together with the ... | 2010 | 20461624 |
| enhancing the virulence of paecilomyces lilacinus against meloidogyne incognita eggs by overexpression of a serine protease. | to enhance the virulence of paecilomyces lilacinus against meloidogyne incognita eggs, a serine protease was overexpressed in p. lilacinus 9410 (pl9410). the cdna of a mature serine protease gene was cloned from pl9410 and integrated into the genomes of pl9410 transformants through agrobacterium tumefaciens-mediated transformation. our results confirmed that the serine protease gene was overexpressed at the transcriptional level, and that the serine protease activities were enhanced in the trans ... | 2010 | 20473777 |
| differential gene expression in arabidopsis following infection by plant-parasitic nematodes meloidogyne incognita and heterodera schachtii. | summary whole genome microarrays were used to study plant gene expression in mature meloidogyne incognita-induced galls in arabidopsis. we found 959 genes to be significantly differentially expressed, and two-thirds of these were down-regulated. microarray results were confirmed by qrt-pcr. the temporal and spatial responses of four differentially expressed genes were analysed using gus reporter plants following infection with m. incognita and the cyst nematode heterodera schachtii. the ammonium ... | 2007 | 20507524 |
| elevated co2 changes the interactions between nematode and tomato genotypes differing in the ja pathway. | interactions between the root-knot nematode meloidogyne incognita and three isogenic tomato (lycopersicon esculentum) genotypes were examined when plants were grown under ambient (370 ppm) and elevated (750 ppm) co2. we tested the hypothesis that, defence-recessive genotypes tend to allocate 'extra' carbon (relative to nitrogen) to growth under elevated co2, whereas defence-dominated genotypes allocate extra carbon to defence, and thereby increases the defence against nematodes. for all three ge ... | 2010 | 20519018 |
| phytochemistry and nematicidal activity of the essential oils from 8 greek lamiaceae aromatic plants and 13 terpene components. | eight essential oils (eos) as well as 13 single terpenes were studied for their nematicidal activity against meloidogyne incognita , for three immersion periods (24, 48, and 96 h). the eos were isolated from eight greek lamiaceae species: melissa officinalis , sideritis clandestina , origanum dictamnus , ocimum basilicum , mentha pulegium , origanum vulgare , vitex agnus castus , and salvia officinalis . the eos nematicidal activity was correlated to their chemical composition as well as to the ... | 2010 | 20527965 |
| feeding cell development by cyst and root-knot nematodes involves a similar early, local and transient activation of a specific auxin-inducible promoter element. | summary to study the role of the phytohormone auxin in nematode feeding cell induction and early development, the transcriptional regulation of the artificial auxin-responsive promoter element dr5 was monitored in arabidopsis thaliana roots infected with the cyst nematode heterodera schachtii or the root-knot nematode meloidogyne incognita. for both nematode species, a specific and strong activation of dr5::gusa was observed inside the initial feeding cells at 18 h post inoculation, pointing to ... | 2004 | 20565601 |
| the promoter of the arabidopsis thaliana cel1 endo-1,4-beta glucanase gene is differentially expressed in plant feeding cells induced by root-knot and cyst nematodes. | summary transgenic tobacco and arabidopsis thaliana carrying the arabidopsis endo-1,4-beta-glucanase (ec 3.2.1.4) cel1 promoter fused to the beta-glucuronidase (gus) reporter gene were infected with the root-knot nematode, meloidogyne incognita, and either the tobacco cyst nematode, globodera tabacum (tobacco), or beet cyst nematode, heterodera schachtii (arabidopsis). cel1-driven gus expression was detected in cell elongation zones of noninfected plants and within feeding sites (giant-cells) in ... | 2004 | 20565607 |
| use of solid-phase subtractive hybridization for the identification of parasitism gene candidates from the root-knot nematode meloidogyne incognita. | summary a solid-phase subtractive strategy was used to clone parasitism gene candidates (pgcs) expressed in the oesophageal gland cells of meloidogyne incognita. nematode intestinal first-strand cdna was synthesized directly on magnetic beads and used to enrich for gland-specific sequences by high stringency hybridization to gland-cell mrna. a gland-specific cdna library was created from the nonhybridizing gland-cell mrna by long-distance reverse transcription polymerase chain reaction. subtract ... | 2004 | 20565611 |
| two chorismate mutase genes from the root-knot nematode meloidogyne incognita. | summary parasitism genes encoding secretory proteins expressed in the oesophageal glands of phytoparasitic nematodes play critical roles in nematode invasion of host plants, establishment of feeding sites and suppression of host defences. two chorismate mutase (cm) genes potentially having a role in one or more of these processes were identified from a meloidogyne incognita oesophageal gland-cell subtractive cdna library. these m. incognita enzymes (designated as mi-cm-1 and mi-cm-2) with amino- ... | 2005 | 20565635 |
| differential activation of abi3 and lea genes upon plant parasitic nematode infection. | summary promoter activity of abi3 and of three lea genes was monitored in arabidopsis transgenics infected with heterodera schachtii and meloidogyne incognita. abi3::gus expression was induced (in four different promoter deletion constructs) during early infection stages with h. schachtii. similar gus expression patterns, though slightly later in time compared with abi3, were observed for one of the lea promoter constructs, whereas the other two were not induced by h. schachtii. expression was m ... | 2005 | 20565660 |
| the potato virus y m(s)n(r) nib-replicase is the elicitor of a veinal necrosis-hypersensitive response in root knot nematode resistant tobacco. | summary a root knot nematode resistance gene in nicotiana tabacum, rk, providing resistance to the nematode parasite meloidogyne incognita is tightly linked to, or is a pleiotropic gene with a veinal necrosis systemic hypersensitive response to infection by potato virus y (pvy) m(s)n(r). the single pvy m(s)n(r) open reading frame was sequenced and found to have 89% protein identity to pvy n. individual pvy m(s)n(r) polypeptides were deduced and the corresponding cdna were cloned into a potato vi ... | 2002 | 20569320 |
| identification of cytokinins produced by the plant parasitic nematodes heterodera schachtii and meloidogyne incognita. | summary the presence of different types of cytokinins was analysed in exudates and lysates of stage-2 juveniles of heterodera schachtii and meloidogyne incognita and in mixed stages of caenorhabditis elegans. for all species, cytokinins were detected in lysates and exudates in which benzyladenine and zeatin-type cytokinins were the most prominent forms. the production of cytokinins by meloidogyne was much higher than by heterodera, and the detected levels were in a range which interfered with th ... | 2003 | 20569387 |
| nematodes as vectors to introduce agrobacterium into plant roots. | summary a fast plant promoter test was developed by means of a nematode to transfer agrobacterium tumefaciens into plant roots. two-week-old arabidopsis thaliana (l.) heynh. plants were transferred to infection medium. meloidogyne incognita or heterodera schachtii juveniles were mixed with the agrobacterium strain that harboured the binary vector, and this mixture was used for plant inoculation. during migration of the nematode and establishment of the feeding site inside the roots, the t-dna wa ... | 2000 | 20572986 |
| post-transcriptional gene silencing of root-knot nematode in transformed soybean roots. | rnai constructs targeted to four different genes were examined to determine their efficacy to reduce galls formed by meloidogyne incognita in soybean roots. these genes have high similarity with essential soybean cyst nematode (heterodera glycines) and caenorhabditis elegans genes. transformed roots were challenged with m. incognita. two constructs, targeted to genes encoding tyrosine phosphatase (tp) and mitochondrial stress-70 protein precursor (msp), respectively, strongly interfered with m. ... | 2011 | 20599433 |
| ssr markers closely associated with genes for resistance to root-knot nematode on chromosomes 11 and 14 of upland cotton. | molecular markers closely linked to genes that confer a high level of resistance to root-knot nematode (rkn) [meloidogyne incognita (kofoid & white) chitwood] in cotton (gossypium hirsutum l.) germplasm derived from auburn 623 rnr would greatly facilitate cotton breeding programs. our objectives were to identify simple sequence repeat (ssr) markers linked to rkn resistance quantitative trait loci (qtl) and map these markers to specific chromosomes. we developed three recombinant inbred line (ril ... | 2010 | 20607210 |
| fine mapping qmi-c11 a major qtl controlling root-knot nematodes resistance in upland cotton. | the identification and utilization of a high-level of host plant resistance is the most effective and economical approach to control root-knot nematode (meloidogyne incognita). in an earlier study, we identified a major quantitative trait locus (qtl) for resistance to root-knot nematode in the m-120 rnr upland cotton line (gossypium hirsutum l.) of the auburn 623 rnr source. the qtl is located in a 12.9-cm interval flanked by the two ssr markers cir069 and cir316 on the distal segment of chromos ... | 2010 | 20680610 |
| geostatistical modeling of the spatial variability and risk areas of southern root-knot nematodes in relation to soil properties. | identifying the spatial variability and risk areas for southern root-knot nematode [meloidogyne incognita (kofoid & white) chitwood] (rkn) is key for site-specific management (ssm) of cotton (gossypium hirsutum l.) fields. the objectives of this study were to: (i) determine the soil properties that influence rkn occurrence at different scales; and (ii) delineate risk areas of rkn by indicator kriging. the study site was a cotton field located in the southeastern coastal plain region of the usa. ... | 2010 | 20717481 |
| cytotoxic tirucallane triterpenoids from melia azedarach fruits. | the phytochemical investigation of the dichloromethane-soluble part of the methanol extract obtained from the fruits of melia azedarach afforded one new tirucallane-type triterpene, 3-alpha-tigloylmelianol and three known tirucallanes, melianone, 21-beta-acetoxy-melianone, and methyl kulonate. the structure of the isolated compounds was mainly determined by 1d and 2d nmr experiments as well as hplc-q-tof mass spectrometry. the cytotoxicity of the isolated compounds toward the human lung adenocar ... | 2010 | 20802401 |
| effect of meloidogyne incognita inoculum density and application rate of paecilomyces lilacinus strain 251 on biocontrol efficacy and colonization of egg masses analyzed by real-time quantitative pcr. | the fungal biocontrol agent, paecilomyces lilacinus strain 251 (pl251), was evaluated for its potential to control the root-knot nematode meloidogyne incognita on tomato at varying application rates and inoculum densities. conversely to previous studies, significant dose-response relationships could not be established. however, we demonstrated that a preplanting soil treatment with the lowest dose of commercially formulated pl251 (2 × 10(5) cfu/g soil) was already sufficient to reduce root galli ... | 2011 | 20822430 |
| chemotaxis can take plant-parasitic nematodes to the source of a chemo-attractant via the shortest possible routes. | it has long been recognized that chemotaxis is the primary means by which nematodes locate host plants. nonetheless, chemotaxis has received scant attention. we show that chemotaxis is predicted to take nematodes to a source of a chemo-attractant via the shortest possible routes through the labyrinth of air-filled or water-filled channels within a soil through which the attractant diffuses. there are just two provisos: (i) all of the channels through which the attractant diffuses are accessible ... | 2010 | 20880854 |
| nematicidal carboxylic acids and aldehydes from melia azedarach fruits. | melia azedarach is a species gaining scientific interest mostly concerning its range of biological activities against agricultural target pests. the nematicidal melia methanol extract (mme) obtained from the fruits, acting against the phytonematode meloidogyne incognita , is herein reported to contain hexadecanoic, acetic, and hexanoic acids as well as furfural, 5-hydroxymethylfurfural, 5-methylfurfural, and furfurol. all compounds were tested individually for nematicidal activity against the ne ... | 2010 | 20925380 |
| data-mining of the meloidogyne incognita degradome and comparative analysis of proteases in nematodes. | proteases perform essential physiological functions in all living organisms. in parasitic helminths, they are of particular importance for tissue penetration, digestion of host tissues for nutrition, and evasion of host immune responses. the recent availability of the genome sequence of the nematode meloidogyne incognita has allowed the analysis of the protease repertoire of this major crop pathogen. the m. incognita degradome consists of at least 334 proteases that are distributed into 43 famil ... | 2011 | 20951198 |
| genome-wide survey and analysis of microsatellites in nematodes, with a focus on the plant-parasitic species meloidogyne incognita. | microsatellites are the most popular source of molecular markers for studying population genetic variation in eukaryotes. however, few data are currently available about their genomic distribution and abundance across the phylum nematoda. the recent completion of the genomes of several nematode species, including meloidogyne incognita, a major agricultural pest worldwide, now opens the way for a comparative survey and analysis of microsatellites in these organisms. | 2010 | 20973953 |
| peroxiredoxins from the plant parasitic root-knot nematode, meloidogyne incognita, are required for successful development within the host. | root-knot nematodes, meloidogyne spp., are sedentary biotrophic parasites which are able to infest > 2000 plant species. after root invasion they settle sedentarily inside the vascular cylinder and maintain a compatible interaction for up to 8 weeks. plant cells respond to pathogen attacks by producing reactive oxygen species (ros). these ros, in particular hydroperoxides, are important regulators of host-parasite interactions and partly govern the success or failure of disease. ros producing an ... | 2011 | 21145323 |
| toward a mechanistic understanding of competition in vascular-feeding herbivores: an empirical test of the sink competition hypothesis. | recent evidence suggests that competitive interactions among herbivores are mostly indirect and mediated by plant responses to herbivory. most studies, however, emphasize chewing insects and secondary chemistry, thus ignoring the diverse group of vascular-parasites that may be more likely to compete through induced changes in phytonutrients. using an aboveground phloem-feeding aphid (myzus persicae) and a belowground gall-forming nematode (meloidogyne incognita) on tobacco plants, we assessed th ... | 2010 | 21181415 |
| evolution of nematode-resistant mi-1 gene homologs in three species of solanum. | plants have evolved several defense mechanisms, including resistance genes. resistance to the root-knot nematode meloidogyne incognita has been found in wild plant species. the molecular basis for this resistance has been best studied in the wild tomato solanum peruvianum and it is based on a single dominant gene, mi-1.2, which is found in a cluster of seven genes. this nematode attacks fiercely several crops, including potatoes. the genomic arrangement, number of copies, function and evolution ... | 2011 | 21207065 |
| expression profile of jasmonic acid-induced genes and the induced resistance against the root-knot nematode (meloidogyne incognita) in tomato plants (solanum lycopersicum) after foliar treatment with methyl jasmonate. | we investigated what gene(s) in the plant roots have the positive role against repressing root-knot nematode (rkn) infection. we investigated the interaction between rkn infection and gene expression in the plant roots induced by methyl jasmonate (meja). we focused on the induced resistance response and the duration after foliar treatment with meja of 0.1, 0.5, 1.0, and 5.0mm at 1, 24, 48, and 72h prior to the inoculation of rkn. as a result, the foliar treatment with meja at 0.5mm or higher con ... | 2011 | 21216026 |
| synergistic and antagonistic interactions of terpenes against meloidogyne incognita and the nematicidal activity of essential oils from seven plants indigenous to greece. | biorational means for phytonematode control were studied within the context of an increasingly ecofriendly pest management global approach. the nematicidal activity and the chemical composition of essential oils (eos) isolated from seven plants grown in greece and ten selected compounds extracted from them against second-stage juveniles (j2) of meloidogyne incognita (kof. & white) chitwood were evaluated using juvenile paralysis experiments. additionally, synergistic and antagonistic interaction ... | 2011 | 21308960 |
| potential use of cucumber (cucumis sativus l.) endophytic fungi as seed treatment agents against root-knot nematode meloidogyne incognita. | seed treatment with endophytic fungi has been regarded as an effective method for plant parasitic nematode control. endophytic fungi from cucumber seedlings were isolated and screened for their potential to be used as seed treatment agents against meloidogyne incognita. among the 294 isolates screened, 23 significantly reduced galls formed by m. incognita in greenhouse test. the 10 most effective isolates were fusarium (5), trichoderma (1), chaetomium (1), acremonium (1), paecilomyces (1), and p ... | 2011 | 21370507 |
| identifying discriminative classification-based motifs in biological sequences. | identification of conserved motifs in biological sequences is crucial to unveil common shared functions. many tools exist for motif identification, including some that allow degenerate positions with multiple possible nucleotides or amino acids. most efficient methods available today search conserved motifs in a set of sequences, but do not check for their specificity regarding to a set of negative sequences. | 2011 | 21372086 |
| in vitro proteolysis of nematode fmrfamide-like peptides (flps) by preparations from a free-living nematode ( panagrellus redivivus) and two plant-parasitic nematodes ( heterodera glycines and meloidogyne incognita). | proteolytic activities in extracts from three nematodes, the plant parasites heterodera glycines and meloidogyne incognita, and the free-living panagrellus redivivus, were surveyed for substrate preferences using a battery of seven fret-modified peptide substrates, all derived from members of the large fmrf-amide like peptide (flp) family in nematodes. overall protease activity in p. redivivus was four- to fivefold greater than in either of the parasites, a result that might reflect developmenta ... | 2011 | 21392420 |
| agrobacterium rhizogenes-mediated transformation of prunus as an alternative for gene functional analysis in hairy-roots and composite plants. | resistant rootstocks offer an alternative to pesticides for the control of soil pests. in prunus spp., resistance loci to root-knot nematodes (rkn) have been mapped and a transformation method is needed to validate candidate genes. our efforts have focused on the generation of transformed hairy-roots and composite plants appropriate for nematode infection assays. an efficient and reliable method using the a4r strain of agrobacterium rhizogenes for the transformation of prunus roots with an egfp ... | 2011 | 21409553 |
| ectopic expression of a meloidogyne incognita dorsal gland protein in tobacco accelerates the formation of the nematode feeding site. | meloidogyne spp., plant-parasitic nematodes present worldwide, are intensively studied because of the damage caused to a large variety of agronomically important crops. several reports indicate that proteins from the meloidogyne spp. dorsal gland might play an important role to allow proper establishment of a functional nematode feeding site. the precise role of these proteins in the process of feeding cell development is unknown. to gain insights into the function of these secreted m. incognita ... | 2011 | 21421371 |
| effect of methomyl and oxamyl soil applications on early control of nematodes and insects. | methomyl is a widely used carbamate insecticide that has traditionally been applied as a foliar spray. more recently, methomyl has been labeled as a soil application via drip chemigation. not much is known about the insecticidal and nematicidal potential of soil-applied methomyl. methomyl soil applications were evaluated for their potential to control soil nematodes and foliar insect pests in a series of lab and greenhouse tests. | 2011 | 21438120 |
| histological mechanisms of the resistance conferred by the ma gene against meloidogyne incognita in prunus spp. | the ma gene from myrobalan plum is a tnl gene that confers a high-level resistance to all root-knot nematodes of major economic importance, including meloidogyne incognita, m. javanica, m. arenaria, and m. enterolobii. the nematode behavior in the roots and the corresponding histological mechanisms of the ma resistance to m. incognita in the resistant (r) accessions of the plum 'p.2175' and the interspecific hybrid p.2175×almond-peach '35', carrying the ma1 allele (ma1/ma), were characterized in ... | 2011 | 21446787 |
| expression of arabidopsis pathogenesis-related genes during nematode infection. | the expression pattern of pathogenesis-related genes pr-1 to pr-5 was examined in the roots and leaves of arabidopsis thaliana plants on infection with beet-cyst (heterodera schachtii) and root-knot (meloidogyne incognita) nematodes. during h. schachtii parasitism of arabidopsis, the expression of pr-1, pr-2 and pr-5, which are considered to be markers for salicylic acid (sa)-dependent systemic acquired resistance (sar), was induced in both roots and leaves of infected plants. in addition, the e ... | 2011 | 21453430 |
| low cryoprotectant concentrations and fast cooling for nematode cryostorage. | cryopreservation protocols based on slow freezing or vitrification often result in cell injury due to ice formation, cell dehydration and/or toxic concentrations of cryoprotectant (cpa). in this study, we present a cryopreservation technique based on low, non-toxic concentrations of cryoprotectants (≈ 2-4m) combined with a rapid cooling rate in the liquid nitrogen phase (-196°c). protocols for successfully cryopreserving the plant parasitic nematodes globodera tabacum tabacum, heterodera schacht ... | 2011 | 21524646 |
| management of root knot nematode, meloidogyne incognita in cucumber (cucumis sativus) using silicon. | silicon (si) has been reported to effectively manage some pests and diseases of plants. this study was conducted to determine the effect of si concentration, mode, and frequency of application in managing meloidogyne incognita in cucumber. a susceptible cultivar of cucumber (cv. cyclone) was planted in pots containing heat-sterilized soil. three weeks after planting, the plants were inoculated with 1,000 juveniles/ pot. uninoculated plants were provided to serve as control. three concentrations ... | 2010 | 21539270 |
| transcriptome analysis shows differential gene expression in the saprotrophic to parasitic transition of pochonia chlamydosporia. | expression profiles were identified in the fungus pochonia chlamydosporia, a biological control agent of plant parasitic nematodes, through a cdna-amplified fragment length polymorphism approach. two isolates with different host ranges, imi 380407 and imi 331547, were assayed in conditions of saprotrophic-to-parasitic transition, through in vitro assays. gene expression profiles from three different nutritional conditions and four sampling times were generated, with eggs of host nematodes globod ... | 2011 | 21541788 |
| chemical constituents of cordia latifolia and their nematicidal activity. | following nematicidal activity-guided isolation studies on the fruits, bark, and leaves of cordia latifolia, two new constituents, cordinoic acid (=11-oxours-12-ene-23,28-dioic acid; 1) and cordicilin (=2-{[(e)-3-(3,4-dihydroxyphenyl)prop-2-enoyl]oxy}-3-[4-hydroxy-3-(stearoyloxy)phenyl]propanoic acid; 2) were isolated from the stem and leaves, respectively, together with nine known compounds, namely cordioic and cordifolic acid from the stem bark, latifolicin a-d and rosmarinic acid from the fru ... | 2011 | 21560233 |
| analysis of gene expression in soybean (glycine max) roots in response to the root knot nematode meloidogyne incognita using microarrays and kegg pathways. | root-knot nematodes are sedentary endoparasites that can infect more than 3000 plant species. root-knot nematodes cause an estimated $100 billion annual loss worldwide. for successful establishment of the root-knot nematode in its host plant, it causes dramatic morphological and physiological changes in plant cells. the expression of some plant genes is altered by the nematode as it establishes its feeding site. | 2011 | 21569240 |
| elevated co2 influences nematode-induced defense responses of tomato genotypes differing in the ja pathway. | rising atmospheric co(2) concentrations can affect the induced defense of plants against chewing herbivores but little is known about whether elevated co(2) can change the induced defense of plants against parasitic nematodes. this study examined the interactions between the root-knot nematode meloidogyne incognita and three isogenic tomato (lycopersicon esculentum) genotypes grown under ambient (390 ppm) and elevated (750 ppm) co(2) in growth chambers. in a previous study with open-top chambers ... | 2011 | 21629688 |
| aliphatic ketones from ruta chalepensis (rutaceae) induce paralysis on root knot nematodes. | this paper reports on the use of ruta chalepensis l. extracts as a potential nematicide against root knot nematodes meloidogyne incognita and meloidogyne javanica . the essential oil (reo) and methanol extract (rme) of r. chalepensis were tested against second-stage juveniles, with reo inducing paralysis in both species (ec(50/1d) = 77.5 and 107.3 mg/l) and rme being selective for m. incognita (ec(50/1d) = 1001 mg/l). chemical characterization of extracts was done by means of gc-ms and lc-ms, re ... | 2011 | 21631118 |
| rnai effector diversity in nematodes. | while rna interference (rnai) has been deployed to facilitate gene function studies in diverse helminths, parasitic nematodes appear variably susceptible. to test if this is due to inter-species differences in rnai effector complements, we performed a primary sequence similarity survey for orthologs of 77 caenorhabditis elegans rnai pathway proteins in 13 nematode species for which genomic or transcriptomic datasets were available, with all outputs subjected to domain-structure verification. our ... | 2011 | 21666793 |
| nematocidal flavone-c-glycosides against the root-knot nematode (meloidogyne incognita) from arisaema erubescens tubers. | a screening of several chinese medicinal herbs for nematicidal properties showed that arisaema erubescens (wall.) schott tubers possessed significant nematicidal activity against the root-knot nematode (meloidogyne incognita). from the ethanol extract, two nematicidal flavone-c-glycosides were isolated by bioassay-guided fractionation. the compounds were identified as schaftoside and isoschaftoside on the basis of their phytochemical and spectral data. schaftoside and isoschaftoside possessed st ... | 2011 | 21694672 |
| transgenic tobacco plants constitutively expressing arabidopsis npr1 show enhanced resistance to root-knot nematode, meloidogyne incognita. | abstract: in arabidopsis, non-expressor of pathogenesis related genes-1, npr1 has been shown to be a positive regulator of the salicylic acid controlled systemic acquired resistance pathway and modulates the cross talk between sa and ja signaling. transgenic plants expressing atnpr1 constitutively exhibited resistance against pathogens as well as herbivory. in the present study, tobacco transgenic plants expressing atnpr1 were studied further for their response to infection by the sedentary endo ... | 2011 | 21722410 |