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effect of volatile organic compounds from bacteria on nematodes.the five studied bacterial strains could produce volatile organic compounds (vocs) that kill nematodes. based on their 16s rrna sequences, these strains were identified as pseudochrobactrum saccharolyticum, wautersiella falsenii, proteus hauseri, arthrobacter nicotianae, and achromobacter xylosoxidans. the bacterial vocs were extracted using solid-phase micro-extraction (spme) and subsequently identified by gc/ms analysis. the vocs covered a wide range of aldehydes, ketones, alkyls, alcohols, al ...201526363885
management of root-knot nematode (meloidogyne incognita) in bottle gourd using different botanicals in pots.a pot experiment was conducted to study the efficacy of different botanicals in varying doses for management of root-knot nematode, m. incognita in bottle gourd. the results exhibited that madar (calotropis procera) and neem (azadirachta indica) leaves application proved to be more effective in improving plant growth characters and reducing root-knot index and final nematode population. among the doses tested, higher dose of 1.5 % (w/w) was more effective than lower ones.201526345048
de novo analysis of the transcriptome of pratylenchus zeae to identify transcripts for proteins required for structural integrity, sensation, locomotion and parasitism.the root lesion nematode pratylenchus zeae, a migratory endoparasite, is an economically important pest of major crop plants (e.g. cereals, sugarcane). it enters host roots, migrates through root tissues and feeds from cortical cells, and defends itself against biotic and abiotic stresses in the soil and in host tissues. we report de novo sequencing of the p. zeae transcriptome using 454 flx, and the identification of putative transcripts encoding proteins required for movement, response to stim ...201626292651
interaction between meloidogyne incognita and rhizoctonia solani on green beans.the interaction between meloidogyne incognita (race 2) and rhizoctonia solani (ag 4) in a root rot disease complex of green beans (phaseolus vulgaris) was examined in a greenhouse pot experiment. three week-old seedlings (cv. contender) were inoculated with the nematode and/or the fungus in different combinations and sequences. two months after last nematode inoculation, the test was terminated and data were recorded. the synchronized inoculation by both pathogens (n + f) increased the index of ...201526288560
identification of micrornas in meloidogyne incognita using deep sequencing.micrornas play important regulatory roles in eukaryotic lineages. in this paper, we employed deep sequencing technology to sequence and identify micrornas in m. incognita genome, which is one of the important plant parasitic nematodes. we identified 102 m. incognita microrna genes, which can be grouped into 71 nonredundant mirnas based on mature sequences. among the 71 mirans, 27 are known mirnas and 44 are novel mirnas. we identified seven mirna clusters in m. incognita genome. four of the seve ...201526241472
genetic analysis of verticillium wilt resistance in a backcross inbred line population and a meta-analysis of quantitative trait loci for disease resistance in cotton.verticillium wilt (vw) and fusarium wilt (fw), caused by the soil-borne fungi verticillium dahliae and fusarium oxysporum f. sp. vasinfectum, respectively, are two most destructive diseases in cotton production worldwide. root-knot nematodes (meloidogyne incognita, rkn) and reniform nematodes (rotylenchulus reniformis, rn) cause the highest yield loss in the u.s. planting disease resistant cultivars is the most cost effective control method. numerous studies have reported mapping of quantitative ...201526239843
meloidogyne incognita - rice (oryza sativa) interaction: a new model system to study plant-root-knot nematode interactions in monocotyledons.plant-parasitic nematodes developed strategies to invade and colonize their host plants, including expression of immune suppressors to overcome host defenses. meloidogyne graminicola and m. incognita are root-knot nematode (rkn) species reported to damage rice (oryza sativa l.) cultivated in upland and irrigated systems. despite m. incognita wide host range, study of the molecular plant - rkn interaction has been so far limited to a few dicotyledonous model plants. the aim of this study was to i ...201426224554
mi-1-mediated nematode resistance in tomatoes is broken by short-term heat stress but recovers over time.tomato (solanum lycopersicum l.) is among the most valuable agricultural products, but meloidogyne spp. (root-knot nematode) infestations result in serious crop losses. in tomato, resistance to root-knot nematodes is controlled by the gene mi-1, but heat stress interferes with mi-1-associated resistance. inconsistent results in published field and greenhouse experiments led us to test the effect of short-term midday heat stress on tomato susceptibility to meloidogyne incognita race 1. under cont ...201526170475
yellow pigment aurovertins mediate interactions between the pathogenic fungus pochonia chlamydosporia and its nematode host.nematophagous fungi are globally distributed soil fungi and well-known natural predators of soil-dwelling nematodes. pochonia chlamydosporia can be found in diverse nematode-suppressive soils as a parasite of nematode eggs and is one of the most studied potential biological control agents of nematodes. however, little is known about the functions of small molecules in the process of infection of nematodes by this parasitic fungus or about small-molecule-mediated interactions between the pathogen ...201526151481
synthesis and nematicidal activities of 1,2,3-benzotriazin-4-one derivatives against meloidogyne incognita.a series of novel 1,2,3-benzotriazin-4-one derivatives were synthesized by the reaction of 3-bromoalkyl-1,2,3-benzotriazin-4-ones with potassium salt of 2-cyanoimino-4-oxothiazolidine in the presence of potassium iodide. nematicidal assays in vivo showed that some of them exhibited good control efficacy against the cucumber root-knot nematode disease caused by meloidogyne incognita, up to 100% at the concentration of 10.0 mg l(-1), which indicated that 1,2,3-benzotriazin-4-one derivatives might ...201526145729
complete genome sequence of bacillus thuringiensis yc-10, a novel active strain against plant-parasitic nematodes.bacillus thuringiensis is an important microbial biopesticide for controlling agricultural pests by the production of toxic parasporal crystals proteins.here,we report the finished annotated genome sequence of b. thuringiensis yc-10,which is highly toxic to nematodes.the complete genome sequence consists of a circular chromosome and nine circular plasmids,which the biggest plasmid harbors six parasporal crystals proteins genes consisting of cry1aa, cry1ac, cry1ia, cry2aa, cry2ab and cryb1. the c ...201526100235
systemic acquired resistance activation in solanaceous crops as a management strategy against root-knot nematodes.activators of systemic acquired resistance (sar), such as salicylic acid (sa) and its synthetic functional analogues benzo(1,2,3)thiadiazole-7-carbothionic acid-s-methyl ester (bth) and 2,6-dichloroisonicotinic acid (ina), were tested on tomato, eggplant and pepper for the control of the root-knot nematode meloidogyne incognita. effects on plant fitness, nematode reproduction and root galling were screened in relation to different methods of application, to different applied dosages of chemicals ...201626085141
nematicidal activity of the volatilome of eruca sativa on meloidogyne incognita.research on new pesticides based on plant extracts, aimed at the development of nontoxic formulates, has recently gained increased interest. this study investigated the use of the volatilome of rucola (eruca sativa) as a powerful natural nematicidal agent against the root-knot nematode, meloidogyne incognita. analysis of the composition of the volatilome, using gc-ms-spme, showed that the compound (z)-3-hexenyl acetate was the most abundant, followed by (z)-3-hexen-1-ol and erucin, with relative ...201526082278
a nematode calreticulin, rs-crt, is a key effector in reproduction and pathogenicity of radopholus similis.radopholus similis is a migratory plant-parasitic nematode that causes severe damage to many agricultural and horticultural crops. calreticulin (crt) is a ca2+-binding multifunctional protein that plays key roles in the parasitism, immune evasion, reproduction and pathogenesis of many animal parasites and plant nematodes. therefore, crt is a promising target for controlling r. similis. in this study, we obtained the full-length sequence of the crt gene from r. similis (rs-crt), which is 1,527-bp ...201526061142
proteome of soybean seed exudates contains plant defense-related proteins active against the root-knot nematode meloidogyne incognita.several studies have described the effects of seed exudates against microorganisms, but only few of them have investigated the proteins that have defensive activity particularly against nematode parasites. this study focused on the proteins released in the exudates of soybean seeds and evaluated their nematicidal properties against meloidogyne incognita. a proteomic approach indicated the existence of 63 exuded proteins, including β-1,3-glucanase, chitinase, lectin, trypsin inhibitor, and lipoxy ...201526034922
identification of quantitative trait loci underlying resistance to southern root-knot and reniform nematodes in soybean accession pi 567516c.soybean cyst nematode (scn, heterodera glycine ichinohe), southern root-knot nematode [srkn, meloidogyne incognita (kofoid and white) chitwood] and reniform nematode (rn, rotylenchulus reniformis linford and oliveira) are three important plant-parasitic pests in soybean. previous study showed that plant introduction (pi) 567516c harbored novel quantitative trait loci (qtl) conferring scn resistance to soybean. however, qtl underlying resistance to srkn and rn in pi 567516c remain unknown. the ob ...201726028986
the role of a phospholipase (pld) in virulence of purpureocillium lilacinum (paecilomyces lilacinum).phospholipases are key enzymes in pathogenic fungi that cleave host phospholipids, resulting in membrane destabilization and host cell penetration. however, understanding the role of phospholipases on the virulence of the filamentous fungus purpureocillium lilacinum has been still rather limited. in this study, pld gene was characterized. it encodes the protein phospholipase d (pld) in p. lilacinum. this gene, 3303 bp open reading frame fragment (orf), encodes a protein of 1100 amino acids with ...201526026833
knock-down of heat-shock protein 90 and isocitrate lyase gene expression reduced root-knot nematode reproduction.crop losses caused by nematode infections are estimated to be valued at usd 157 billion per year. meloidogyne incognita, a root-knot nematode (rkn), is considered to be one of the most important plant pathogens due to its worldwide distribution and the austere damage it can cause to a large variety of agronomically important crops. rna interference (rnai), a gene silencing process, has proven to be a valuable biotechnology alternative method for rkn control. in this study, the rnai approach was ...201526020830
the plant cell inhibitor krp6 is involved in multinucleation and cytokinesis disruption in giant-feeding cells induced by root-knot nematodes.the plant cell cycle inhibitor gene krp6 has been investigated in roots infected by plant-parasitic root-knot nematodes (meloidogyne spp.). unexpectedly, krp6 overexpressing lines revealed a distinct role for this specific krp as an activator of the mitotic cell cycle. this function was confirmed in arabidopsis thaliana suspension cultures ectopically expressing krp6. a blockage in the mitotic exit was observed in cell suspensions and in giant cells resulted in the appearance of multi-nucleated ...201525915833
involvement of nitric oxide in the jasmonate-dependent basal defense against root-knot nematode in tomato plants.jasmonic acid (ja) and nitric oxide (no) are well-characterized signaling molecules in plant defense responses. however, their roles in plant defense against root-knot nematode (rkn, meloidogyne incognita) infection are largely unknown. in this study, we found that the transcript levels of the ja- and no-related biosynthetic and signaling component genes were induced after rkn infection. application of exogenous ja and sodium nitroprusside (snp; a no donor) significantly decreased the number of ...201525914698
tomato transgenic plants expressing hairpin construct of a nematode protease gene conferred enhanced resistance to root-knot nematodes.root-knot nematodes (meloidogyne incognita) cause substantial yield losses in vegetables worldwide, and are difficult to manage. continuous withdrawal of environmentally-harmful nematicides from the global market warrants the need for novel nematode management strategies. utility of host-delivered rnai has been demonstrated in several plants (arabidopsis, tobacco, and soybean) that exhibited resistance against root-knot and cyst nematodes. herein, a m. incognita-specific protease gene, cathepsin ...201525883594
suitability of zucchini and cucumber genotypes to populations of meloidogyne arenaria, m. incognita, and m. javanica.the host suitability of five zucchini and three cucumber genotypes to meloidogyne incognita (mipm26) and m. javanica (mj05) was determined in pot experiments in a greenhouse. the number of egg masses (em) did not differ among the genotypes of zucchini or cucumber, but the eggs/plant and reproduction factor (rf) did slightly. m. incognita mipm26 showed lower em, eggs/plant, and rf than m. javanica mj05. examination of the zucchini galls for nematode postinfection development revealed unsuitable c ...201525861120
development of polymorphic microsatellites for meloidogyne incognita, through screening predicted microsatellite loci based on genome sequence.microsatellites are extensively distributed in the eukaryotic genome, and they are widely used for their high polymorphism and accessibility. the microsatellites in m. incognita, a worldwide agriculture pest, are inadequate for diversity research. a repertoire of 1620 microsatellites appeared appropriate to design primer as markers were identified based on the m. incognita genome. 120 loci were chosen as candidate, from which 88 microsatellites were characterized. finally, we found 13 polymorphi ...201525857200
correction to nematicidal activities of 4-quinolone alkaloids isolated from the aerial part of triumfetta grandidens against meloidogyne incognita. 201525830595
transcriptome analysis of resistant and susceptible alfalfa cultivars infected with root-knot nematode meloidogyne incognita.nematodes are one of the major limiting factors in alfalfa production. root-knot nematodes (rkn, meloidogyne spp.) are widely distributed and economically important sedentary endoparasites of agricultural crops and they may inflict significant damage to alfalfa fields. as of today, no studies have been published on global gene expression profiling in alfalfa infected with rkn or any other plant parasitic nematode. very little information is available about molecular mechanisms that contribute to ...201525822722
associated bacteria of different life stages of meloidogyne incognita using pyrosequencing-based analysis.the root knot nematode (rkn), meloidogyne incognita, belongs to the most damaging plant pathogens worldwide, and is able to infect almost all cultivated plants, like tomato. recent research supports the hypothesis that bacteria often associated with plant-parasitic nematodes, function as nematode parasites, symbionts, or commensal organisms etc. in this study, we explored the bacterial consortia associated with m. incognita at different developmental stages, including egg mass, adult female and ...201525809195
cowpea-meloidogyne incognita interaction: root proteomic analysis during early stages of nematode infection.cowpea (vigna unguiculata l. walp) is an important legume species well adapted to low fertility soils and prolonged drought periods. one of the main problems that cause severe yield losses in cowpea is the root-knot nematode meloidogyne incognita. the aim of this work was to analyze the differential expression of proteins in the contrasting cultivars of cowpea ce 31 (highly resistant) and ce 109 (slightly resistant) during early stages of m. incognita infection. cowpea roots were collected at 3, ...201525736976
transformation of the endophytic fungus acremonium implicatum with gfp and evaluation of its biocontrol effect against meloidogyne incognita.acremonium implicatum is an endophytic fungus with biocontrol potential against meloidogyne incognita based on its opportunistic egg-parasitic, hatching inhibition, and toxic properties. to understand its mode of plant endophytism and opportunistic egg parasitism, gfp-tagged a. implicatum was constructed by peg-mediated protoplast transformation. by laser scanning confocal microscopy (lscm), we evaluated the endophytism and opportunistic egg parasitism of a stable gfp transformant (acr-1). acr-1 ...201525724297
evaluation of entomopathogenic nematodes and the supernatants of the in vitro culture medium of their mutualistic bacteria for the control of the root-knot nematodes meloidogyne incognita and m. arenaria.the suppressive effects of various formulations of four entomopathogenic nematode (epn) species and the supernatants of their mutualistic bacteria on the root-knot nematodes (rkns) meloidogyne incognita and m. arenaria in tomato roots were evaluated. the epns steinernema carpocapsae, s. feltiae, s. glaseri and heterorhabditis bacteriophora were applied as either live infective juveniles (ijs) or infected insect cadavers. spent medium from culturing the bacterial symbionts xenorhabdus bovienii an ...201625721911
transcriptome analysis of resistant and susceptible alfalfa cultivars infected with root-knot nematode meloidogyne incognita.nematodes are one of the major limiting factors in alfalfa production. root-knot nematodes (rkn, meloidogyne spp.) are widely distributed and economically important sedentary endoparasites of agricultural crops and they may inflict significant damage to alfalfa fields. as of today, no studies have been published on global gene expression profiling in alfalfa infected with rkn or any other plant parasitic nematode. very little information is available about molecular mechanisms that contribute to ...201525710378
systemic nematicidal activity and biocontrol efficacy of bacillus firmus against the root-knot nematode meloidogyne incognita.a strain of marine bacterium bacillus firmus ybf-10 with nematicidal activity was originally isolated by our group. in the present study, the systemic nematicidal activity and biocontrol efficacy in pot experiment of b. firmus ybf-10 were investigated. our results showed that ybf-10 exhibits systemic nematicidal activity against meloidogyne incognita, including lethal activity, inhibition of egg hatch and motility. pot experiment suggested that soil drenching with ybf-10 efficiently reduced dama ...201525672545
mitochondrial genome plasticity among species of the nematode genus meloidogyne (nematoda: tylenchina).the mitochondrial (mt) genomes of the plant-parasitic root-knot nematodes meloidogyne arenaria, meloidogyne enterolobii and meloidogyne javanica were sequenced and compared with those of three other root-knot nematode species in order to explore the mt genome plasticity within meloidogyne. the mt genomes of m. arenaria, m. enterolobii and m. javanica are circular, with an estimated size of 18.8, 18.9 and 19.6 kb, respectively. compared to other nematodes these mt genomes are larger, due to the p ...201525655462
nematicidal activity of acetophenones and chalcones against meloidogyne incognita and structure-activity considerations.with the ultimate goal of identifying new compounds active against root-knot nematodes, a set of 14 substituted chalcones were synthesised, starting from acetophenones. these chalcones and various acetophenones were tested in vitro against meloidogyne incognita.201625641877
maturation of nematode-induced galls in medicago truncatula is related to water status and primary metabolism modifications.root-knot nematodes are obligatory plant parasitic worms that establish and maintain an intimate relationship with their host plants. during a compatible interaction, these nematodes induce the redifferentiation of root cells into multinucleate and hypertrophied giant cells (gcs). these metabolically active feeding cells constitute the exclusive source of nutrients for the nematode. we analyzed the modifications of water status, ionic content and accumulation of metabolites in development and ma ...201525617326
expression of phenylalanine ammonia lyase genes in maize lines differing in susceptibility to meloidogyne incognita.maize is a well-known host for meloidogyne incognita, and there is substantial variation in host status among maize genotypes. in previous work it was observed that nematode reproduction increased in the moderately susceptible maize inbred line b73 when the zmlox3 gene from oxylipid metabolism was knocked out. additionally, in this mutant line, use of a nonspecific primer for phenyl alanine ammonialyase (pal) genes indicated that expression of these genes was reduced in the mutant maize plants w ...201425580029
physiological effects of meloidogyne incognita infection on cotton genotypes with differing levels of resistance in the greenhouse.greenhouse tests were conducted to evaluate (i) the effect of meloidogyne incognita infection in cotton on plant growth and physiology including the height-to-node ratio, chlorophyll content, dark-adapted quantum yield of photosystem ii, and leaf area; and (ii) the extent to which moderate or high levels of resistance to m. incognita influenced these effects. cultivars fibermax 960 br (susceptible to m. incognita) and stoneville 5599 br (moderately resistant) were tested together in three trials ...201425580028
response of resistant and susceptible bell pepper (capsicum annuum) to a southern california meloidogyne incognita population from a commercial bell pepper field.to determine the presence and level of root-knot nematode (meloidogyne spp.) infestation in southern california bell pepper (capsicum annuum) fields, soil and root samples were collected in april and may 2012 and analyzed for the presence of root-knot nematodes. the earlier samples were virtually free of root-knot nematodes, but the later samples all contained, sometimes very high numbers, of root-knot nematodes. nematodes were all identified as m. incognita. a nematode population from one of th ...201425580027
site specific nematode management-development and success in cotton production in the united states.variability in edaphic factors such as clay content, organic matter, and nutrient availability within individual fields is a major obstacle confronting cotton producers. adaptation of geospatial technologies such global positioning systems (gps), yield monitors, autosteering, and the automated on-and-off technology required for site-specific nematicide application has provided growers with additional tools for managing nematodes. multiple trials in several states were conducted to evaluate this ...201425580023
pyramiding taro cystatin and fungal chitinase genes driven by a synthetic promoter enhances resistance in tomato to root-knot nematode meloidogyne incognita.meloidogyne incognita, one of the major root-knot nematode (rkn) species in agriculture, attacks many plant species, causing severe economic losses. genetic engineering of plants with defense-responsive genes has been demonstrated to control rkn. these studies, however, focused on controlling rkn at certain growth stages. in the present study, a dual gene overexpression system, utilizing a plant cysteine proteinase inhibitor (cecpi) and a fungal chitinase (pjchi-1), was used to transform tomato ...201525575993
the application of arabidopsis thaliana in studying tripartite interactions among plants, beneficial fungal endophytes and biotrophic plant-parasitic nematodes.the research demonstrated that arabidopsis can be used as a model system for studying plant-nematode-endophyte tripartite interactions; thus, opening new possibilities for further characterizing the molecular mechanisms behind these interactions. arabidopsis has been established as an important model system for studying plant biology and plant-microbe interactions. we show that this plant can also be used for studying the tripartite interactions among plants, the root-knot nematode meloidogyne i ...201525548000
in vitro antagonistic activity, plant growth promoting traits and phylogenetic affiliation of rhizobacteria associated with wild plants grown in arid soil.the role of plant growth-promoting rhizobacteria (pgpr) in adaptation of plants in extreme environments is not yet completely understood. for this study native bacteria were isolated from rhizospeheric arid soils and evaluated for both growth-promoting abilities and antagonistic potential against phytopathogenic fungi and nematodes. the phylogentic affiliation of these representative isolates was also characterized. rhizobacteria associated with 11 wild plant species from the arid soil of almadi ...201425538687
control of meloidogyne incognita using mixtures of organic acids.this study sought to control the root-knot nematode (rkn) meloidogyne incognita using benign organo-chemicals. second-stage juveniles (j2) of rkn were exposed to dilutions (1.0%, 0.5%, 0.2%, and 0.1%) of acetic acid (aa), lactic acid (la), and their mixtures (mx). the nematode bodies were disrupted severely and moderately by vacuolations in 0.5% of mx and single organic acids, respectively, suggesting toxicity of mx may be higher than aa and la. the mortality of j2 was 100% at all concentrations ...201425506312
apoplastic venom allergen-like proteins of cyst nematodes modulate the activation of basal plant innate immunity by cell surface receptors.despite causing considerable damage to host tissue during the onset of parasitism, nematodes establish remarkably persistent infections in both animals and plants. it is thought that an elaborate repertoire of effector proteins in nematode secretions suppresses damage-triggered immune responses of the host. however, the nature and mode of action of most immunomodulatory compounds in nematode secretions are not well understood. here, we show that venom allergen-like proteins of plant-parasitic ne ...201425500833
nematicidal activities of 4-quinolone alkaloids isolated from the aerial part of triumfetta grandidens against meloidogyne incognita.the methanol extract of the aerial part of triumfetta grandidens (tiliaceae) was highly active against meloidogyne incognita, with second-stage juveniles (j2s) mortality of 100% at 500 μg/ml at 48 h post-exposure. two 4-quinolone alkaloids, waltherione e (1), a new alkaloid, and waltherione a (2), were isolated and identified as nematicidal compounds through bioassay-guided fractionation and instrumental analysis. the nematicidal activities of the isolated compounds against m. incognita were eva ...201525494674
overexpression of mic-3 indicates a direct role for the mic gene family in mediating upland cotton (gossypium hirsutum) resistance to root-knot nematode (meloidogyne incognita).transgene-based analysis of the mic-3 gene provides the first report of a cotton gene having a direct role in mediating cotton resistance to root-knot nematode. major quantitative trait loci have been mapped to upland cotton (gossypium hirsutum l.) chromosomes 11 and 14 that govern the highly resistant phenotype in response to infection by root-knot nematode (rkn; meloidogyne incognita); however, nearly nothing is known regarding the underlying molecular determinants of this rkn-resistant phenot ...201525376794
screening and histopathological characterization of korean carrot lines for resistance to the root-knot nematode meloidogyne incognita.in total, 170 carrot lines developed in korea were screened for resistance to meloidogyne incognita race 1 to select parental genetic resources useful for the development of nematode-resistant carrot cultivars. using the gall index (gi), gall formation was examined on carrot roots inoculated with approximately 1,000 second-stage juveniles of the nematode 7 weeks after inoculation. sixty-one carrot lines were resistant (gi ≤ 1.0), while the other 109 were susceptible (gi > 1.0) with coefficient o ...201425288988
the feeding behavior of adult root-knot nematodes (meloidogyne incognita) in rose balsam and tomato.meloidogyne incognita is a parasitic root-knot nematode that causes considerable yield loss in a wide range of plants. in this study we documented the movement of adult female nematodes for more than 2 hr in micro-slices of infected tomato (solanum lycopersicum) and rose balsam (impatiens balsamina) plants using light and video microscopy. stylet thrusting was followed by short pumping actions of the esophagus, dorsal esophageal gland ampulla, and metacorpal bulb. regular thrusting was normally ...201425276004
evaluation of 31 potential biofumigant brassicaceous plants as hosts for three meloiodogyne species.brassicaceous cover crops can be used for biofumigation after soil incorporation of the mowed crop. this strategy can be used to manage root-knot nematodes (meloidogyne spp.), but the fact that many of these crops are host to root-knot nematodes can result in an undesired nematode population increase during the cultivation of the cover crop. to avoid this, cover crop cultivars that are poor or nonhosts should be selected. in this study, the host status of 31 plants in the family brassicaceae for ...201425276003
identification of msp1 gene variants in populations of meloidogyne incognita using pcr-dgge.effectors of root-knot nematodes are essential for parasitism and prone to recognition by adapted variants of the host plants. this selective pressure initiates hypervariability of effector genes. diversity of the gene variants within nematode populations might correlate with host preferences. in this study we developed a method to compare the distribution of variants of the effector gene msp1 among populations of meloidogyne incognita. primers were designed to amplify a 234-bp fragment of msp1. ...201425276001
mitochondrial haplotype-based identification of ethanol-preserved root-knot nematodes from africa.the asexual root-knot nematodes (rkns) (meloidogyne spp.) exemplified by meloidogyne incognita are widespread and damaging pests in tropical and subtropical regions worldwide. comparison of amplification products of two adjacent polymorphic regions of the mitochondrial genome using dna extracts of characterized rkn strains, including 15 different species, indicate that several species are derived from the same or closely related female lineages. nevertheless, m. javanica, m. enterolobii, m. inco ...201525271352
nematicidal activity of paecilomyces lilacinus 6029 cultured on karanja cake medium.antagonistic fungi parasitize root-knot nematodes through secretion of extracellular hydrolytic enzymes and secondary metabolites. in present study, in vitro bioassay showed that paecilomyces lilacinus 6029 culture filtrate from karanja cake medium killed 100% meloidogyne incognita larvae while only 78.28% mortality was recorded by czapeck-dox filtrate within 12 h of exposure. the filtrate, irrespective of culture medium, was found to be more nematotoxic when incubated for 15 days. fourier trans ...201425193498
the dual effects of root-cap exudates on nematodes: from quiescence in plant-parasitic nematodes to frenzy in entomopathogenic nematodes.to defend themselves against herbivores and pathogens, plants produce numerous secondary metabolites, either constitutively or de novo in response to attacks. an intriguing constitutive example is the exudate produced by certain root-cap cells that can induce a state of reversible quiescence in plant-parasitic nematodes, thereby providing protection against these antagonists. the effect of such root exudates on beneficial entomopathogenic nematodes (epns) remains unclear, but could potentially i ...201525165149
benzothiadiazole effect in the compatible tomato-meloidogyne incognita interaction: changes in giant cell development and priming of two root anionic peroxidases.bth application is effective in root-knot nematode-tomato interaction in a way that involves a delay in the formation of nematode feeding site and triggers molecular responses at several levels. the compatible interaction between root-knot nematodes and their hosts requires the nematode to overcome plant defense systems so that a sophisticated permanent feeding site (giant cells) can be produced within the host roots. it has been suggested that activators of plant defenses may provide a novel ma ...201425085693
ovicidal activity of lactic acid produced by lysobacter capsici ys1215 on eggs of root-knot nematode, meloidogyne incognita.lysobacter capsici ys1215 isolated from soil previously showed nematicidal potential for biological control of the root-knot nematode. in this study, lactic acid, a nematicidal compound, was isolated from culture filtrate of ys1215, and its ovicidal activity was investigated. purification and identification of lactic acid were performed by a series of column chromatographies and identified by (1)h and (13)c nmr spectra and gc-ms analysis. our results showed that bacterial culture filtrate contai ...201425085571
characterization of the reniform nematode genome by shotgun sequencing.the reniform nematode (rn), a major agricultural pest particularly on cotton in the united states, is among the major plant-parasitic nematodes for which limited genomic information exists. in this study, over 380 mb of sequence data were generated from pooled dna of four adult female rns and assembled into 67,317 contigs, including 25,904 (38.5%) predicted coding contigs and 41,413 (61.5%) noncoding contigs. most of the characterized repeats were of low complexity (88.9%), and 0.9% of the conti ...201425036535
effect of supreme 15 cs against meloidogyne incognita on tomato.pot and laboratory experiments were conducted to study the efficacy of supreme 15 cs in varying doses for management of root- knot nematode, m. incognita in tomato. the results indicated that supreme 15 cs containing alkali metal n-(n-propyl) dithiocarbamate proved to be more effective in improving plant growth characters and reducing root-knot index and final nematode population. among the doses tested, higher dose of 0.5 % (w/w) was more effective than lower doses.201425035599
development of enzyme linked immunosorbent assay (elisa) for the detection of root-knot nematode meloidogyne incognita.root-knot nematodes (meloidogyne incognita) are obligate, sedentary plant endoparasites that are extremely polyphagous in nature and cause severe economic losses in agriculture. hence, it is essential to control the parasite at an early stage. for any control strategy to be effective, an early and accurate diagnosis is of paramount importance. immunoassays have the inherent advantages of sensitivity and specificity; have the potential to identify and quantify these plant-parasitic nematodes. hen ...201425035590
effect of simultaneous water deficit stress and meloidogyne incognita infection on cotton yield and fiber quality.both water deficit stress and meloidogyne incognita infection can reduce cotton growth and yield, and drought can affect fiber quality, but the effect of nematodes on fiber quality is not well documented. to determine whether nematode parasitism affects fiber quality and whether the combined effects of nematode and drought stress on yield and quality are additive (independent effects), synergistic, or antagonistic, we conducted a study for 7 yr in a field infested with m. incognita. a split-plot ...201424987162
management of meloidogyne incognita with chemicals and cultivars in cotton in a semi-arid environment.management of meloidogyne incognita (root-knot nematode) in cotton in the united states was substantially affected by the decision to stop production of aldicarb by its principle manufacturer in 2011. the remaining commercially available tools to manage m. incognita included soil fumigation, nematicide seed treatments, postemergence nematicide application, and cultivars partially resistant to m. incognita. small plot field studies were conducted on a total of nine sites from 2011-2013 to examine ...201424987161
tulipaline a: structure-activity aspects as a nematicide and v-atpase inhibitor.carbonyl groups are known to form covalent adducts with endogenous proteins, but so far, their nematicidal mechanism of action of has been overlooked. the nematicidal activity of ten lactones was tested in vitro against the root knot nematodes meloidogyne incognita and meloidogynearenaria. in particular, the saturated lactones α-methylene-γ-butyrolactone or tulipaline a (1) and γ-butyrolactone (3) were active against m. incognita with an ec50/48h of 19.3±10.0 and 40.0±16.2mg/l respectively. more ...201424974115
management of meloidogyne incognita on tomato with endophytic bacteria and fresh residue of wasabia japonica.to characterize the nematicidal endophytic bacteria (neb) of wasabia japonica (wasabi) and evaluated the control efficacies of promising neb as well as fresh wasabi residue (fwr) against meloidogyne incognita on tomato.201424965702
the cyclin-dependent kinase inhibitor krp6 induces mitosis and impairs cytokinesis in giant cells induced by plant-parasitic nematodes in arabidopsis.in arabidopsis thaliana, seven cyclin-dependent kinase (cdk) inhibitors have been identified, designated interactors of cdks or kip-related proteins (krps). here, the function of krp6 was investigated during cell cycle progression in roots infected by plant-parasitic root-knot nematodes. contrary to expectations, analysis of meloidogyne incognita-induced galls of krp6-overexpressing lines revealed a role for this particular krp as an activator of the mitotic cell cycle. in accordance, krp6-overe ...201424963053
elucidating the molecular bases of epigenetic inheritance in non-model invertebrates: the case of the root-knot nematode meloidogyne incognita.root-knot nematodes of the genus meloidogyne are biotrophic plant parasites that exhibit different life cycles and reproduction modes, ranging from classical amphimixis to obligatory mitotic parthenogenesis (apomixis), depending on the species. meloidogyne incognita, an apomictic species, exhibits a worldwide distribution and a wide host range affecting more than 3000 plant species. furthermore, evidences suggest that apomixis does not prevent m. incognita from adapting to its environment in con ...201424936189
a distinct role of pectate lyases in the formation of feeding structures induced by cyst and root-knot nematodes.pectin in the primary plant cell wall is thought to be responsible for its porosity, charge density, and microfibril spacing and is the main component of the middle lamella. plant-parasitic nematodes secrete cell wall-degrading enzymes that macerate the plant tissue, facilitating the penetration and migration within the roots. in sedentary endoparasitic nematodes, these enzymes are released only during the migration of infective juveniles through the root. later, nematodes manipulate the express ...201424905398
mining novel effector proteins from the esophageal gland cells of meloidogyne incognita.meloidogyne incognita is one of the most economically damaging plant pathogens in agriculture and horticulture. identifying and characterizing the effector proteins which m. incognita secretes into its host plants during infection is an important step toward finding new ways to manage this pest. in this study, we have identified the cdnas for 18 putative effectors (i.e., proteins that have the potential to facilitate m. incognita parasitism of host plants). these putative effectors are secretory ...201424875667
a meloidogyne incognita effector is imported into the nucleus and exhibits transcriptional activation activity in planta.root-knot nematodes are sedentary biotrophic endoparasites that maintain a complex interaction with their host plants. nematode effector proteins are synthesized in the oesophageal glands of nematodes and secreted into plant tissue through a needle-like stylet. effectors characterized to date have been shown to mediate processes essential for nematode pathogenesis. to gain an insight into their site of action and putative function, the subcellular localization of 13 previously isolated meloidogy ...201524863562
the complex hybrid origins of the root knot nematodes revealed through comparative genomics.root knot nematodes (rkn) can infect most of the world's agricultural crop species and are among the most important of all plant pathogens. as yet however we have little understanding of their origins or the genomic basis of their extreme polyphagy. the most damaging pathogens reproduce by obligatory mitotic parthenogenesis and it has been suggested that these species originated from interspecific hybridizations between unknown parental taxa. we have sequenced the genome of the diploid meiotic p ...201424860695
manipulation of two α-endo-β-1,4-glucanase genes, atcel6 and gmcel7, reduces susceptibility to heterodera glycines in soybean roots.plant endo-β-1,4-glucanases (egases) include cell wall-modifying enzymes that are involved in nematode-induced growth of syncytia (feeding structures) in nematode-infected roots. egases in the α- and β-subfamilies contain signal peptides and are secreted, whereas those in the γ-subfamily have a membrane-anchoring domain and are not secreted. the arabidopsis α-egase at1g48930, designated as atcel6, is known to be down-regulated by beet cyst nematode (heterodera schachtii) in arabidopsis roots, wh ...201424844661
exploitation of microbes for enhancing bacoside content and reduction of meloidogyne incognita infestation in bacopa monnieri l.despite the vast exploration of rhizospheric microbial wealth for crop yield enhancement, knowledge about the efficacy of microbial agents as biocontrol weapons against root-knot disease is scarce, especially in medicinal plants, viz., bacopa monnieri. in the present investigation, rhizospheric microbes, viz., bacillus megaterium, glomus intraradices, trichoderma harzianum thu, and their combinations were evaluated for the management of meloidogyne incognita (kofoid and white) chitwood and bacos ...201524841892
isolation and characterisation of rhizosphere bacteria active against meloidogyne incognita, phytophthora nicotianae and the root knot-black shank complex in tobacco.the use of dually antagonistic bacteria (dab) as alternatives to chemicals for biological control of disease complexes has received little attention. in this study targeting the meloidogyne incognita-phytophthora nicotianae complex, dab from the tobacco rhizosphere were identified and screened against the diseases caused by one or both pathogens in tobacco.201524799254
sirnas trigger efficient silencing of a parasitism gene in plant parasitic root-knot nematodes.expanding genomic data on plant pathogens open new perspectives for the development of specific and environment friendly pest management strategies based on the inhibition of parasitism genes that are essential for the success of infection. identifying such genes relies on accurate reverse genetics tools and the screening of pathogen knock-down phenotypes. root-knot nematodes are major cosmopolitan crop pests that feed on a wide range of host plants. small interfering rnas (sirnas) would provide ...201224704976
bacterial antagonists of fungal pathogens also control root-knot nematodes by induced systemic resistance of tomato plants.the potential of bacterial antagonists of fungal pathogens to control the root-knot nematode meloidogyne incognita was investigated under greenhouse conditions. treatment of tomato seeds with several strains significantly reduced the numbers of galls and egg masses compared with the untreated control. best performed bacillus subtilis isolates sb4-23, mc5-re2, and mc2-re2, which were further studied for their mode of action with regard to direct effects by bacterial metabolites or repellents, and ...201424587352
efficacy evaluation of fungus syncephalastrum racemosum and nematicide avermectin against the root-knot nematode meloidogyne incognita on cucumber.the root-knot nematode (rkn) is one of the most damaging agricultural pests.effective biological control is need for controlling this destructive pathogen in organic farming system. during october 2010 to 2011, the nematicidal effects of the syncephalastrum racemosum fungus and the nematicide, avermectin, alone or combined were tested against the rkn (meloidogyne incognita) on cucumber under pot and field condition in china. under pot conditions, the application of s. racemosum alone or combined ...201424586982
histopathological response of lens culinaris roots towards root-knot nematode, meloidogyne incognito.lens culinaris (lentil) is an important pulse crop. the yield of the crop is reduced if grown in root-knot nematode (meloidogyne incognita) infested field. meloidogyne incognita caused infection in primary and the secondary roots leading to the anomalies in the affected part of the root. the study revealed that the second stage juveniles (j2) of meloidogyne incognita entered the growing roots and their branches inter and intracellularly. the immediate response was hypertrophy and hyperplasia in ...201324498798
isolation and characterization of a rhizobacterial antagonist of root-knot nematodes.the rhizobacterial strain jdm2 was isolated from the rhizosphere of the traditional chinese medicinal herb trichosanthes kirilowii in jiangsu province, china, and was identified as bacillus subtilis. exposure of cell-free filtrate of the strain to the root-knot nematode meloidogyne incognita under in vitro conditions caused substantial mortality of the second stage juvenile (j2) and significantly reduced egg hatchability. a greenhouse trial demonstrated that 56 days after treatment with jdm2, th ...201424465828
chitosan-cinnamon beads enhance suppressive activity against rhizoctonia solani and meloidogyne incognita in vitro.a novel chitosan-cinnamon bead carrier was prepared in this study. chitosan was mixed with cinnamon powder (cp) and cinnamon extract (ce) to obtain chitosan-cinnamon powder (ccp) beads and chitosan-cinnamon extracted (cce) beads, respectively. the potential antifungal and nematicidal activities of ccp and cce were investigated against rhizoctonia solani and meloidogyne incognita in vitro. relative antifungal activity of the ccp (5% cp) bead-treated r. solani was 30.9 and 23.9% after 1 and 2 day ...201424417978
comparison of two sympatric pasteuria populations isolated from a tropical vertisol soil.an isolate of pasteuria (designated ppmj) recovered from the root-knot nematode meloidogyne javanica, was characterized using host preference, spore morphometrics, and serology, and compared with another sympatric pasteuria isolate (designated pphc) collected from the cyst nematode, heterodera cajani. ppmj spores were larger (x 1.5) than the pphc spores and had a mean diameter of 3.4 μm after fixation for electron microscopy. the central body of ppmj spores was about twice as big as the central ...199624415311
knocking-down meloidogyne incognita proteases by plant-delivered dsrna has negative pleiotropic effect on nematode vigor.the root-knot nematode meloidogyne incognita causes serious damage and yield losses in numerous important crops worldwide. analysis of the m. incognita genome revealed a vast number of proteases belonging to five different catalytic classes. several reports indicate that m. incognita proteases could play important roles in nematode parasitism, besides their function in ordinary digestion of giant cell contents for feeding. the precise roles of these proteins during parasitism however are still u ...201324392004
characterization of thermo- and detergent stable antigenic glycosylated cysteine protease of euphorbia nivulia buch.-ham. and evaluation of its ecofriendly applications.an antigenic glycosylated cysteine protease has been purified from the latex of euphorbia nivulia buch.-ham. it exhibits remarkable protease activity in the presence of metal ions, oxidizing agents, organic solvents, and detergents. this enzyme showed potential role in leather processing industry due to its dehairing activity for animal hide without hydrolyzing fibrous proteins, producing, by this way, a better quality product. the enzyme can also be used for silver recovering from x-ray plates. ...201324348183
inheritance and mapping of mj-2, a new source of root-knot nematode (meloidogyne javanica) resistance in carrot.root-knot nematodes limit carrot production around the world by inducing taproot forking and galling deformities that render carrots unmarketable. in warmer climates, meloidogyne javanica and meloidogyne incognita are most prevalent. in f2 and f3 progeny from the cross between an asian carrot resistant to m. javanica, pi 652188, and a susceptible carrot, resistance response was incompletely dominant with a relatively high heritability (h (2) = 0.78) and provided evidence for a single gene, desig ...201424336925
effects of meloidogyne incognita and thielaviopsis basicola on cotton growth and root morphology.effects of the root-knot nematode meloidogyne incognita and the fungal pathogen thielaviopsis basicola on cotton seedling growth and root morphology were evaluated in controlled environmental experiments. four pathogen treatments, including noninfested soil, soil infested with m. incognita, soil infested with t. basicola, and soil infested with both pathogens were evaluated at soil bulk densities (bds) of 1.25 and 1.50 g/cm(3). plant growth and the morphology of the root systems were evaluated 4 ...201424283540
effects of chloroform extract of dryopteris crassirhizoma on the ultramicroscopic structures of meloidogyne incognita.in our early experiments, the chloroform extract of d. crassirhizoma was demonstrated to contain the highest concentrations of total phloroglucinols among several extract fractions and possessed the most effective nematicidal activity. this study aimed to ascertain the ultrastructural changes in m. incognita after treatment with a d. crassirhizoma chloroform extract at 1 mg·ml⁻¹ for 24 h. it was found that the extract exhibited significant destructive effects on the worm's ultrastructure and cau ...201324282379
α-tomatine and resistance of tomato cultivars toward the nematode,meloidogyne incognita.susceptible and resistant tomato cultivars were assayed for root tomatine concentrations at different developmental times. no correlation between high tomatine levels and resistance towardmeloidogyne incognita was apparent. in plants infected with nematodes, tomatine in the roots was the same as in uninfected controls. tomatine concentration, as measured by bulk analysis, does not appear to play an important role in resistance toward the nematode studied.198824276208
strong repellency of the root knot nematode,meloidogyne incognita by specific inorganic ions.simple inorganic salts of the ions k(+), nh 4 (+) , cs(+), no 3 (-) , and cl(-) are strongly repellent to infective second-stage larvae of the root knot nematode,meloidogyne incognita. some of these salts are known to be beneficial to plant growth. the results suggest a new means of plant protection.199024263720
utility of host delivered rnai of two fmrf amide like peptides, flp-14 and flp-18, for the management of root knot nematode, meloidogyne incognita.root knot nematode, meloidogyne incognita, is an obligate sedentary endoparasite that infects a large number of crop species and causes substantial yield losses. non-chemical based control strategies for these nematodes are gaining importance. in the present study, we have demonstrated the significance of two fmrfamide like peptide genes (flp-14 and flp-18) for infection and development of resistance to m. incognita through host-derived rnai. the study demonstrated both in vitro and in planta va ...201324223228
identification of novel target genes for safer and more specific control of root-knot nematodes from a pan-genome mining.root-knot nematodes are globally the most aggressive and damaging plant-parasitic nematodes. chemical nematicides have so far constituted the most efficient control measures against these agricultural pests. because of their toxicity for the environment and danger for human health, these nematicides have now been banned from use. consequently, new and more specific control means, safe for the environment and human health, are urgently needed to avoid worldwide proliferation of these devastating ...201324204279
inheritance of resistance to meloidogyne spp. in common bean and the genetic basis of its sensitivity to temperature.bean lines pi 165426, pi 165435, and alabama no. 1, possessing resistance to meloidogyne incognita, and bean lines a315 and a445, possessing gene me1, were tested against several meloidogyne incognita and m. javanica isolates. resistance in bean line pi 165426, pi 165435, and alabama no. 1 was found to be complementary to resistance conferred by gene me1. resistance in pi 165426 was found to be dominant and conditioned by one dominant and one recessive gene at 26 °c. we propose me2me3 as the gen ...199224202746
inheritance of heat-stable resistance to meloidogyne incognita in lycopersicon peruvianum and its relationship to the mi gene.the inheritance of heat-stable resistance to the root-knot nematode, meloidogyne incognita (kofoid and white) chitwood, was studied in crosses between different accessions and clones of lycopersicon peruvianum l. f1, f2 and bc1 generations were evaluated for their index of resistance based on numbers of eggs and infective second-stage juveniles (j2) per gram of root, and the segregation ratios were determined in experiments carried out at constant soil temperatures of 25 °c and 30 °c. l. peruvia ...199324196050
[the participation of salicylic and jasmonic acids in genetic and induced resistance of tomato to meloidogyne incognita (kofoid and white, 1919)].salicylic (sa) and jasmonic (ja) acids are the best known mediators of signal systems in plants. in this investigation the participation and character of interactions between sa- and ja-signals under the induced and genetic resistance of plants to nematodes was investigated on the model system tomato (lycopersicon esculentum) and the root-knot nematode meloidogyne incognita. this study demonstrates that application of ja and sa to tomato foliage induces systemic effects that suppress root-knot n ...201624171314
lauric acid in crown daisy root exudate potently regulates root-knot nematode chemotaxis and disrupts mi-flp-18 expression to block infection.tomato (solanum lycopersicum) crops can be severely damaged due to parasitism by the root-knot nematode (rkn) meloidogyne incognita, but are protected when intercropped with crown daisy (chrysanthemum coronarium l.). root exudate may be the determining factor for this protection. an experiment using pots linked by a tube and petri dish experiments were undertaken to confirm that tomato-crown daisy intercropping root exudate decreased the number of nematodes and alleviated nematode damage, and to ...201424170741
differentiation of meloidogyne incognita and m. arenaria novel resistance phenotypes in lycopersicon peruvianum and derived bridge-lines.lycopersicon peruvianum pi 270435 clone 2r2 and pi 126443 clone 1mh were crossed reciprocally with three l. esculentum-l. peruvianum bridge-lines. the incongruity barrier between the two plant species was overcome; f1 progeny were obtained from crosses between four parental combinations without embryo-rescue culture. hybridity was confirmed by leaf and flower morphology and by the production of nematode-resistant f1 progeny on homozygous susceptible parents. clones of the five f1 bridgeline hybr ...199624162431
relationships between meloidogyne incognita resistance genes in lycopersicon peruvianum differentiated by heat sensitivity and nematode virulence.resistance to meloidogyne incognita (kofoid and white) chitwood in clones of lycopersicon peruvianum (l.) mill. pi 126443-1mh, 270435-2r2 and 2704353mh, their f1, a field-produced f2, and their test-cross (tc1) populations, was evaluated based on egg masses and eggs produced on root systems. reactions to m. incognita isolates differing in virulence to gene mi were determined at 25°c (mi expressed) and 32°c (mi not expressed). pi 126443-1mh, 270435-2r2, 270435-3mh, and their f1 progenies were res ...199624162430
activity of chalcones derived from 2,4,5-trimethoxybenzaldehyde against meloidogyne exigua and in silico interaction of one chalcone with a putative caffeic acid 3-o-methyltransferase from meloidogyne incognita.meloidogyne exigua is a parasitic nematode of plants that causes great losses to coffee farmers. in an effort to develop parasitic controls, 154 chalcones were synthesized and screened for activity against this nematode. the best results were obtained with (2e)-1-(4'-nitrophenyl)-3-(2,4,5-trimethoxyphenyl)prop-2-en-1-one (6) with a 50% lethal concentration (lc50) of 171 μg/ml against m. exigua second-stage juveniles, in comparison to the commercially-available nematicide carbofuran which had an ...201324140596
expression of tomato salicylic acid (sa)-responsive pathogenesis-related genes in mi-1-mediated and sa-induced resistance to root-knot nematodes.the expression pattern of pathogenesis-related genes pr-1, pr-2 and pr-5, considered as markers for salicylic acid (sa)-dependent systemic acquired resistance (sar), was examined in the roots and shoots of tomato plants pre-treated with sa and subsequently infected with root-knot nematodes (rkns) (meloidogyne incognita). pr-1 was up-regulated in both roots and shoots of sa-treated plants, whereas the expression of pr-5 was enhanced only in roots. the over-expression of pr-1 in the whole plant oc ...201424118790
nematicidal activity of the essential oil of rhododendron anthopogonoides aerial parts and its constituent compounds against meloidogyne incognita.hydrodistilled essential oil from rhododendron anthopogonoides maxim. (ericaceae) aerial parts was analysed by gas chromatography-mass spectrometry (gc-ms). a total of 42 compounds, accounting for 95.48% of the total oil, were identified. the main constituents of the essential oil were benzyl acetone (34.41%), nerolidol (10.19%), 1,4-cineole (8.41%), beta-caryophyllene (5.63%), gamma-elemene (5.10%), and spathulenol (3.06%). four constituents were isolated from the essential oil based on fractio ...201324066516
enhanced levels of plant cell cycle inhibitors hamper root-knot nematode-induced feeding site development.root-knot nematodes (rkn) are highly specialized, obligatory plant parasites. these animals reprogram root cells to form large, multinucleate, and metabolically active feeding cells (giant cells) that provide a continuous nutrient supply during 3-6 weeks of the nematode's life. the establishment and maintenance of physiologically fully functional giant cells are necessary for the survival of these nematodes. as such, giant cells may be useful targets for applying strategies to reduce damage caus ...201324056043
nematicidal activity of mint aqueous extracts against the root-knot nematode meloidogyne incognita.the nematicidal activity and chemical characterization of aqueous extracts and essential oils of three mint species, namely, mentha × piperita , mentha spicata , and mentha pulegium , were investigated. the phytochemical analysis of the essential oils was performed by means of gc-ms, whereas the aqueous extracts were analyzed by lc-ms. the most abundant terpenes were isomenthone, menthone, menthol, pulegone, and carvone, and the water extracts yielded mainly chlorogenic acid, salvianolic acid b, ...201324050256
the nematicidal effect of some bacterial biofertilizers on meloidogyne incognita in sandy soil.in a greenhouse experiment, the nematicidal effect of some bacterial biofertilizers including the nitrogen fixing bacteria (nfb) paenibacillus polymyxa (four strains), the phosphate solubilizing bacteria (psb) bacillus megaterium (three strains) and the potassium solubilizing bacteria (ksb) b. circulans (three strains) were evaluated individually on tomato plants infested with the root-knot nematode meloidogyne incognita in potted sandy soil. comparing with the uninoculated nematode-infested con ...201124031611
solanum torvum responses to the root-knot nematode meloidogyne incognita.solanum torvum sw is worldwide employed as rootstock for eggplant cultivation because of its vigour and resistance/tolerance to the most serious soil-borne diseases as bacterial, fungal wilts and root-knot nematodes. the little information on solanum torvum (hereafter torvum) resistance mechanisms, is mostly attributable to the lack of genomic tools (e.g. dedicated microarray) as well as to the paucity of database information limiting high-throughput expression studies in torvum.201323937585
exploring the host parasitism of the migratory plant-parasitic nematode ditylenchus destuctor by expressed sequence tags analysis.the potato rot nematode, ditylenchus destructor, is a very destructive nematode pest on many agriculturally important crops worldwide, but the molecular characterization of its parasitism of plant has been limited. the effectors involved in nematode parasitism of plant for several sedentary endo-parasitic nematodes such as heterodera glycines, globodera rostochiensis and meloidogyne incognita have been identified and extensively studied over the past two decades. ditylenchus destructor, as a mig ...201323922743
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