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effect of tea dust residues to control root-knot nematode of tomato.in this research, control of tomato root- knot nematode (meloidogyne incognita) was conducted using tea dust residues at different rates. first, the species and race of nematode were identified by employing diagnostic keys. then, with 5 replications in complete randomized design. tea dust residues were used at 9 treatments (0, 5, 10, 15, 20, 25, 30, 35 and 40 g/kg of soil). statistical analysis on mean treatments rates showed that treatment with 25 g/kg soil economically was effective in growth ...200415759440
management of meloidogyne incognita on tomato by root-dip treatment in culture filtrate of the blue-green alga, microcoleus vaginatus.the nematicidal potential of culture filtrates of the blue-green alga, microcoleus vaginatus (cyanobacterium) was tested against meloidogyne incognita on tomato in pots under greenhouse conditions. prior to the transplantation of tomato seedling, roots were dipped in different concentrations (0.2%, 0.5%, 1%, 2%, 10%, 50% and 100%) of culture filtrate of m. vaginatus for 30 min. root-dip treatment reduced the root galling and final population of m. incognita and increased vegetative growth of pla ...200515792580
developmental expression and molecular analysis of two meloidogyne incognita pectate lyase genes.proteinaceous secretions from the oesophageal glands of plant-parasitic nematodes have crucial roles in nematode parasitism of plants. two cdnas (designated mi-pel-1 and mi-pel-2) encoding pectate lyases were isolated from the root-knot nematode, meloidogyne incognita, oesophageal gland-cell subtractive cdna libraries, and the corresponding genomic dnas were subsequently cloned. southern blot analyses revealed that homologues to these pectate lyase genes were broadly distributed in meloidogyne s ...200515862581
isolation and characterization of a cdna encoding a serine proteinase from the root-knot nematode meloidogyne incognita.this report describes the first serine proteinase gene isolated from the sedentary nematode meloidogyne incognita. using degenerate primers, a 1372bp cdna encoding a chymotrypsin-like serine proteinase (mi-ser1) was amplified from total rna of adult females by rt-pcr and 5' and 3' rapid amplification of cdna ends. the deduced amino acid sequence of mi-ser1 encoded a putative signal peptide and a prodomain of 22 and 33 amino acids, respectively, and a mature proteinase of 341 amino acids with a p ...200515888294
[adaptogenic effects of furostanol glycosides of dioscorea deltoidea wall on oxidative processes in tomato plants in biotic stress].the effect of furostanol glycosides of cell culture of dioscorea deltoidea wall on oxidative processes in tomato plants subjected to invasion with the gall nematode meloidogyne incognita kofoid et white was studied. we showed that furostanol glycosides induce a nonspecific defensive response in plants. exposure of cell membranes to furostanol glycosides cause rearrangements in fatty acids resulting in the formation of conjugated dienes, which makes molecules thermodynamically more stable under s ...201315977797
nematicidal natural products from the aerial parts of lantana camara linn.lantanilic acid, camaric acid and oleanolic acid possessing nematicidal activity were isolated from the methanolic extract of the aerial parts of lantana camara linn. through bio-assay guided fractionation. these compounds exhibited 98%, 95% and 70% mortality respectively against root-knot nematode meloidogyne incognita at 0.5% concentration. conventional nematicide furadan showed 100% mortality at this concentration.200516010828
application of rna interference to root-knot nematode genes encoding esophageal gland proteins.plant parasitic nematodes have been, so far, refractory to transformation or mutagenesis. the functional analysis of nematode genes relies on the development of reverse genetic tools adapted to these obligate parasites. here, we describe the application of rna interference (rnai) to the root-knot nematode meloidogyne incognita for the knock-down of two genes expressed in the subventral esophageal glands of the nematode and potentially involved in parasitism, the calreticulin (mi-crt) and the pol ...200516042006
ex vitro composite plants: an inexpensive, rapid method for root biology.plant transformation technology is frequently the rate-limiting step in gene function analysis in non-model plants. an important tool for root biologists is the agrobacterium rhizogenes-derived composite plant, which has made possible genetic analyses in a wide variety of transformation recalcitrant dicotyledonous plants. the novel, rapid and inexpensive ex vitro method for producing composite plants described in this report represents a significant advance over existing composite plant inductio ...200516045479
studies on the management of root-knot nematode, meloidogyne incognita-wilt fungus, fusarium oxysporum disease complex of green gram, vigna radiata cv ml-1108.studies were conducted under pot conditions to determine the comparative efficacy of carbofuran at 1 mg a.i./kg soil, bavistin at 1 mg a.i./kg soil, neem (azadirachta indica) seed powder at 50 mg/kg soil, green mould (trichoderma harzianum) at 50.0 ml/kg soil, rhizobacteria (pseudomonas fluorescens) at 50.0 ml/kg soil against root-knot nematode, meloidogyne incognita-wilt fungus, fusarium oxysporum disease complex on green gram, vigna radiata cv ml-1108. all the treatments significantly improved ...200516052706
effects of fly ash, pseudomonas striata and rhizobium on the reproduction of nematode meloidogyne incognita and on the growth and transpiration of pea.glasshouse experiments were conducted twice to assess the ash amendments (0, 20, and 40% with soil), a phosphate solubilizing microorganism pseudomonas striata and a root-nodule bacterium rhizobium sp on the reproduction of root-knot nematode meloidogyne incognita and on the growth and transpiration of pea. amendments of fly ash with soil had no effect on transpiration. however, m. incognita reduced the rate of transpiration from 1st week onward after inoculation while inoculation of rhizobium s ...200516114471
extracellular protease of pseudomonas fluorescens cha0, a biocontrol factor with activity against the root-knot nematode meloidogyne incognita.in pseudomonas fluorescens cha0, mutation of the gaca-controlled apra gene (encoding the major extracellular protease) or the gaca regulatory gene resulted in reduced biocontrol activity against the root-knot nematode meloidogyne incognita during tomato and soybean infection. culture supernatants of strain cha0 inhibited egg hatching and induced mortality of m. incognita juveniles more strongly than did supernatants of apra and gaca mutants, suggesting that apra protease contributes to biocontro ...200516151170
rna interference of dual oxidase in the plant nematode meloidogyne incognita.rna interference (rnai) is a powerful tool for the analysis of gene function in model organisms such as the nematode caenorhabditis elegans. recent demonstrations of rnai in plant parasitic nematodes provide a stimulus to explore the potential of using rnai to investigate disruption of gene function in meloidogyne incognita, one of the most important nematode pests of global agriculture. we have used rnai to examine the importance of dual oxidases (peroxidase and nadph oxidase), a class of enzym ...200516255249
colonization by the arbuscular mycorrhizal fungus glomus versiforme induces a defense response against the root-knot nematode meloidogyne incognita in the grapevine (vitis amurensis rupr.), which includes transcriptional activation of the class iii chitinase gene vch3.inoculation of the grapevine (vitis amurensis rupr.) with the arbuscular mycorrhizal (am) fungus glomus versiforme significantly increased resistance against the root-knot nematode (rkn) meloidogyne incognita. studies using relative quantitative reverse transcription-pcr (rqrt-pcr) analysis of grapevine root inoculation with the am fungus revealed an up-regulation of vch3 transcripts. this increase was greater than that observed following infection with rkn. however, inoculation of the mycorrhiz ...200616326755
identification and mapping of microsatellite markers linked to a root-knot nematode resistance gene (rkn1) in acala nemx cotton (gossypium hirsutum l.).host-plant resistance is the most economic and effective strategy for root-knot nematode (rkn) meloidogyne incognita control in cotton (gossypium hirsutum l.). molecular markers linked to resistance are important for incorporating resistance genes into elite cultivars. to screen for microsatellite markers (ssr) closely linked to rkn resistance in g. hirsutum cv. acala nemx, f1, f2, bc1f1, and f2:7 recombinant inbred lines (rils) from intraspecific crosses and an f2 from an interspecific cross wi ...200616362274
production of auxin and related compounds by the plant parasitic nematodes heterodera schachtii and meloidogyne incognita.mass spectrometric analysis revealed the presence of auxin, mainly in conjugated form, in secretions of heterodera schachtii and meloidogyne incognita, with or without treatment with dmt or resorcinol. m. incognita showed the highest production rates, though treatment of m. incognita with resorcinol had a negative effect on auxin production. analysis of auxin precursor molecules in lysates of h. schachtii, m. incognita and caenorhabditis elegans suggested that auxin is most probably a degradatio ...200516363359
impact of the nematophagous fungus pochonia chlamydosporia on nematode and microbial populations.the microbial and nematode populations associated with two plants (tomato and cabbage) inoculated with the nematophagous fungus, pochonia chlamydosporia var. chlamydosporia or root knot nematode (meloidogyne incognita), or both, were compared with those in unplanted controls. the dominant factor affecting culturable microbial populations was found to be the presence or absence of tomato plants. generally microbial colony counts were lowest in unplanted soil, small increases were associated with ...200516363363
a major gene mapped on chromosome xii is the main factor of a quantitatively inherited resistance to meloidogyne fallax in solanum sparsipilum.meloidogyne fallax is an emerging pest in europe and represents a threat for potato production. we report the mapping of genetic factors controlling a quantitative resistance against m. fallax identified in the solanum sparsipilum genotype 88s.329.15. when infected, this genotype develops a necrotic reaction at the feeding site of the juveniles and totally prevents their development to the female stage. a "f1" diploid progeny consisting of 128 individuals was obtained using the potato (s. tubero ...200616365760
morphological variability and molecular phylogeny of the nematophagous fungus monacrosporium drechsleri.an isolate of the nematode-trapping fungus monacrosporium drechsleri was collected from cultures of the root-knot nematode meloidogyne arenaria that had been maintained on tomato roots in greenhouse pots in beltsville, maryland. the plant-parasitic nematodes heterodera glycines, meloidogyne incognita and pratylenchus zeae and the free-living nematodes caenorhabditis elegans and panagrellus redivivus were placed on colonies of m. drechsleri grown in petri dishes to study ability of the isolate to ...200516396348
isolation and characterization of another cdna encoding a chorismate mutase from the phytoparasitic nematode meloidogyne arenaria.a new cdna, named ma-cm-2, encoding a chorismate mutase (cm), has been isolated from meloidogyne arenaria. the full-length cdna, carrying the trans-spliced sl1 leader sequence, was 753-bp long with an open reading frame of 576 bp. the deduced protein ma-cm-2 including amino-terminal signal peptide shows significant similarity to cms of meloidogyne incognita, meloidogyne javanica, and also bacteria. secondary structure prediction of ma-cm-2 indicates the presence of the three conserved alpha-heli ...200616458297
[effects of meloidogyne incognita initial inoculation density on ginger growth].the study with potted ginger showed that meloidogyne incognita infection could reduce the plant height, stem diameter, quantity of shoots, and fresh weight of stem, leaf and root, and finally, reduce ginger yield. with the enhancement of m. incognita initial inoculation density, the growth of ginger was damaged more severely, e. g., under inoculating 50, 100 and 200 m. incognita eggs per 100 g soil, ginger yield decreased by 27.91%, 37.73% and 42.14%, respectively. the reproduction rate of m. in ...200516471354
in planta secretion of a calreticulin by migratory and sedentary stages of root-knot nematode.esophageal secretions from endoparasitic sedentary nematodes are thought to play key roles throughout plant parasitism, in particular during the invasion of the root tissue and the initiation and maintenance of the nematode feeding site (nfs) essential for nematode development. the secretion in planta of esophageal cell-wall-degrading enzymes by migratory juveniles has been shown, suggesting a role for these enzymes in the invasion phase. nevertheless, the secretion of an esophageal gland protei ...200516478047
screening of field pea (pisum sativum) selections for their reactions to root-knot nematode (meloidogyne incognita).pot studies were carried out to evaluate the reactions of 23 selections of field pea (pisum sativum) against meloidogyne incognita (2000 freshly hatched juveniles (j2)/pot). experiment was conducted under greenhouse conditions ((24.7+/-3) degrees c and (62+/-7)% rh) and terminated 45 d after inoculation. the roots of all the selections were assessed to determine root-knot indices (rki) on a 0-4 scale. out of 23 selections hfp-990713, pant p-25, and hfp-0129 were resistant; pant p-2005, ndp-2 and ...200616502508
effects of cina on root-knot disease of mulberry.root-knot disease of mulberry is caused by the nematode meloidogyne incognita. it has important economic implications for sericulture. the homeopathic medicines, cina mother tincture (mt) and potentised cina 200c, prepared from the flowering meristems of artemisia nilagirica (clarke) pamp, were applied by foliar spray on mulberry (morus alba l.) infected with m. incognita juveniles (j2). pretreatment (ending 6 days before inoculation) and post-treatment (starting 6 days after inoculation) schedu ...200616569626
biological control potential of the obligate parasite pasteuria penetransagainst the root-knot nematode, meloidogyne incognita infestation in brinjal.the efficacy of the obligate bacterial parasite, pasteuria penetrans against the rootknot nematode, meloidogyne incognita infestation was assessed in brinjal. the seedling pans with sterilized soil were inoculated with nematodes and root powder of p. penetrans were applied at different dosages viz., 0 x 10(6), 0.5 x 10(6) spores and 1 x 10(6) spores/pan. seeds of brinjal cv co2 were sown in the pans and seedlings were allowed to grow. the seedlings were transplanted to microplots containing ster ...200516628937
integrated management of root-knot nematode, meloidogyne incognita infestation in tomato and grapevine.an integrated approach with the obligate bacterial parasite, pasteuria penetrans and nematicides was assessed for the management of the root-knot nematode, meloidogyne incognita infestation in tomato and grapevine. seedlings of tomato cv. co3 were transplanted into pots filled with sterilized soil and inoculated with nematodes (5000 juveniles/pot). the root powder of p. penetrans at 10 mg/pot was applied alone and in combination with carbofuran at 6 mg/pot. application of p. penetrans along with ...200516628938
a symbiont-independent endo-1,4-beta-xylanase from the plant-parasitic nematode meloidogyne incognita.substituted xylan polymers constitute a major part of the hemicellulose fraction of plant cell walls, especially in monocotyledons. endo-1,4-beta-xylanases (ec 3.2.1.8) are capable of hydrolyzing substituted xylan polymers into fragments of random size. many herbivorous animals have evolved intimate relationships with endosymbionts to exploit their enzyme complexes for the degradation of xylan. here, we report the first finding of a functional endo-1,4-beta-xylanase gene from an animal. the gene ...200616673939
development of controlled release formulations of carbofuran and evaluation of their efficacy against meloidogyne incognita.controlled release (cr) formulations of the insecti-nematicide carbofuran have been prepared using commercially available rosin, sodium carboxymethylcellulose and sodium carboxymethylcellulose with clay (bentonite, kaolinite, and fuller's earth). the kinetics of carbofuran release in soil from the different formulations were studied in comparison with that of the commercially available granules (3g). release from the commercial formulation was faster than with the new cr formulations. addition o ...200616787021
amelioration of root-knot disease of lady's finger plants by potentized cina and santonin.lady's finger plants (hibiscus esculentus), grown in pots, were inoculated with the second-stage larvae (76+/-6) of root-knot nematodes meloidogyne incognita, starting 7 days later they were treated with cina 30c, santonin 30c or ethanol 30c by foliar spray for 10 consecutive days. the drugs in 90% ethanol were diluted with distilled water 1:1000 before application on plants. thirty days after the last treatment the plants were uprooted. cina 30c and santonin 30c reduced nematode infestation of ...200616815517
nematode infection and reproduction in transgenic and mutant arabidopsis and tobacco with an altered phenylpropanoid metabolism.transgenic and mutant arabidopsis and tobacco plants with altered phenylpropanoid metabolism were infected with the plant parasitic root knot nematode meloidogyne incognita to assess the effect of the transgene or mutation on nematode infection and reproduction. modifications in the lignin biosynthetic pathway which alter lignin composition in roots affected reproduction. in arabidopsis with increased levels of syringyl lignin, reproduction was lower than in wild-type plants, while in tobacco wi ...200616831845
gastrodia anti-fungal protein from the orchid gastrodia elata confers disease resistance to root pathogens in transgenic tobacco.diseases of agricultural crops are caused by pathogens from several higher-order phylogenetic lineages including fungi, straminipila, eubacteria, and metazoa. these pathogens are commonly managed with pesticides due to the lack of broad-spectrum host resistance. gastrodia anti-fungal protein (gafp; gastrodianin) may provide a level of broad-spectrum resistance due to its documented anti-fungal activity in vitro and structural similarity to insecticidal lectins. we transformed tobacco (nicotiana ...200616858580
use of crop residues for the control of meloidogyne incognita under laboratory conditions.this laboratory study evaluates the biofumigant effect of different organic materials with the aim of developing non-chemical alternatives for the management of meloidogyne incognita (kofoid & white) chitwood populations. sources of organic material from the production system were selected with the aim of reducing agricultural residue accumulation problems as well as decreasing the costs due to the use of chemical fertilizers and pesticides. the selected materials were residues from pepper, stra ...200616927410
velvetbean (mucuna pruriens) extracts: impact on meloidogyne incognita survival and on lycopersicon esculentum and lactuca sativa germination and growth.velvetbean (mucuna spp.) is a summer annual that has been used as a cover crop to reduce erosion, fix nitrogen and suppress weeds and plant-parasitic nematodes. crude aqueous extracts (1:15 dry weight plant/volume water) were made from velvetbean plant parts, and various concentrations of the extracts were evaluated in vitro for toxicities to different stages of meloidogyne incognita (kofoid and white) chitwood and for suppression of hypocotyl and root growth and inhibition of germination of tom ...200616953489
qtl mapping for resistance to root-knot nematodes in the m-120 rnr upland cotton line (gossypium hirsutum l.) of the auburn 623 rnr source.root-knot nematodes meloidogyne incognita (kofoid and white) can cause severe yield loss in cotton (gossypium hirsutum l.). the objectives of this study were to determine the inheritance and genomic location of genes conferring root-knot nematode resistance in m-120 rnr, a highly resistant g. hirsutum line with the auburn 623 rnr source of resistance. utilizing two interspecific f(2) populations developed from the same m-120 rnr by gossypium barbadense (cv. pima s-6) cross, genome-wide scanning ...200616960714
characterization of a new beta-1,4-endoglucanase gene from the root-knot nematode meloidogyne incognita and evolutionary scheme for phytonematode family 5 glycosyl hydrolases.cellulases from plant parasitic nematodes are encoded by multiple gene families and are thought to originate from horizontal gene transfer. unraveling the evolution of these genes in the phylum will help understanding the evolution of plant parasitism in nematodes. here we describe a new gene, named mi-eng-2, that encodes a family 5 glycosyl hydrolase (ghf5) with a predicted signal peptide and devoid of linker domain and cellulose-binding domain. the beta-1,4-endoglucanase activity of the protei ...200616962258
meloidogyne incognita: molecular and biochemical characterisation of a cathepsin l cysteine proteinase and the effect on parasitism following rnai.rna interference has been used to investigate the function of a cathepsin l cysteine proteinase mi-cpl-1, in the plant-parasitic nematode meloidogyne incognita. a reduction in gene transcript was observed and the number of nematodes infecting plants was reduced by almost 60% as was the number of established females producing eggs at 21 days post-infection. the cysteine proteinase activity of m. incognita, reported by the substrate glupna, was inhibited by the cysteine proteinase inhibitor oc-ide ...200716996059
integrated application of some compatible biocontrol agents along with mustard oil seed cake and furadan on meloidogyne incognita infecting tomato plants.experiments were carried out to study the effect of two fungal bioagents along with mustard oil cake and furadan against root knot nematode meloidogyne incognita infecting tomato under greenhouse condition. bioagents viz., paecilomyces lilacinus and trichoderma viride alone or in combination with mustard cake and furadan promoted plant growth, reduced number of galls/plant, egg masses/root system and eggs/egg mass. the fungal bioagents along with mustard cake and nematicide showed least nematode ...200617048300
atcat6, a sink-tissue-localized transporter for essential amino acids in arabidopsis.amino acids represent the major form of reduced nitrogen that is transported in plants. amino acid transporters in plants often show tissue-specific expression patterns and are used by plants to transport these metabolites from source to sink during development and under changing environmental conditions. we identified one amino acid transporter, atcat6, which is expressed in sink tissues such as lateral root primordia, flowers and seeds. additionally atcat6 was induced during infestation of roo ...200617052324
use of pepper crop residues for the control of root-knot nematodes.the biofumigant effect of pepper crop residues (pcr) for controlling meloidogyne incognita populations was evaluated. under laboratory conditions, 0, 5, 10 and 20 g pcr were applied to 500 g nematode infested soil, with four replicates per treatment. after 20 days at 25 degrees c, pcr reduced significantly m. incognita populations and root galling indices in susceptible tomato cv. marmande, and increased k, n and organic c in soil. in the field, biofumigation with pcr combined with fresh animal ...200717098424
preferential expression of a plant cystatin at nematode feeding sites confers resistance to meloidogyne incognita and globodera pallida.the expression patterns of three promoters preferentially active in the roots of arabidopsis thaliana have been investigated in transgenic potato plants in response to plant parasitic nematode infection. promoter regions from the three genes, tub-1, arsk1 and rpl16a were linked to the gus reporter gene and histochemical staining was used to localize expression in potato roots in response to infection with both the potato cyst nematode, globodera pallida and the root-knot nematode, meloidogyne in ...200417166138
solubilization of insoluble zinc compounds by gluconacetobacter diazotrophicus and the detrimental action of zinc ion (zn2+) and zinc chelates on root knot nematode meloidogyne incognita.to examine the zinc (zn) solubilization potential and nematicidal properties of gluconacetobacter diazotrophicus. methods and results: atomic absorption spectrophotometer, differential pulse polarography and gas chromatography coupled mass spectrometry were used to estimate the total zn and zn(2+) ions and identify the organic acids present in the culture supernatants. the effect of culture filtrate of zn-amended g. diazotrophicus pal5 on meloidogyne incognita in tomato was examined under gnotob ...200717309498
evaluation of a nematode bio-product dbx-20% against root-knot nematode meloidogyne incognita affecting grapevine under field conditions.a field trial was conducted in el-shourouk farm, el-beheira governorate, western nile valley, egypt to determine the effectiveness of the commercial bio-product dbx 1003 20% containing the nematode-trapping fungus dactylaria brochopaga against root-knot nematode meloidogyne incognita infesting grapevine variety superior. its effects on plant growth criteria and yield production were also investigated. the fungus was introduced to soil by either of two ways. first: soil was drenched with spore su ...200617390807
effect of different initial population densities of meloidogyne incognita on the growth of cicer arietinum.studies were undertaken to determine the effect of different initial population densities (pi = 50, 100, 250, 500, 1000, 2500, 5000, 10,000 and 15,000 j2/pot) of meloidogyn incognita on chikpea cv. bandal local in a pot experiment. plant growth, flowering and pod bearing were influenced by all the initial population densities. increase in pi not only resulted in proportional decrease in plant lenght, fresh weight, dry weight and pod bearing but also delayed the flowering. the highest reduction w ...200617390808
use of trichoderma harzianum and trichoderma viride for the biological control of meloidogyne incognita on tomato.trichoderma harzianum and trichoderma viride were tested for their capacity to reduce the incidence of the root-knot nematode meloidogyne incognita on tomato. in vitro studies demonstrated that all tested isolates were effective in causing nematode mortality compared with the control. trichoderma slightly reduced nematode damage to tomato in vivo. treatment of the soil with the biocontrol agents before transplanting, improved control over treatment directly at transplanting. the trichoderma isol ...200617390844
reniform in u.s. cotton: when, where, why, and some remedies.the reniform nematode, rotylenchulus reniformis, is an emerging problem in u.s. cotton. the impact of this nematode and the extent to which it has and will continue to spread across the u.s. cotton belt are controversial. long-term changes in cotton production and unique biological attributes of r. reniformis are key factors. expert opinion surveys indicate that r. reniformis has replaced the root-knot nematode (meloidogyne incognita) as the major nematode of cotton in mississippi, louisiana, an ...200717428179
comprehensive transcriptome profiling in tomato reveals a role for glycosyltransferase in mi-mediated nematode resistance.root-knot nematode (rkn; meloidogyne spp.) is a major crop pathogen worldwide. effective resistance exists for a few plant species, including that conditioned by mi in tomato (solanum lycopersicum). we interrogated the root transcriptome of the resistant (mi+) and susceptible (mi-) cultivars 'motelle' and 'moneymaker,' respectively, during a time-course infection by the mi-susceptible rkn species meloidogyne incognita and the mi-resistant species meloidogyne hapla. in the absence of rkn infectio ...200717434994
coprinus comatus damages nematode cuticles mechanically with spiny balls and produces potent toxins to immobilize nematodes.we reported recently a unique fungal structure, called the spiny ball, on the vegetative hyphae of coprinus comatus (o. f. müll.:fr.) pers. although some observations regarding the role of this structure were presented, its function remained largely unknown. in this study, we showed that purified (isolated and washed) spiny balls could immobilize and kill the free-living nematode panagrellus redivivus goodey highly efficiently. scanning electron microscopy studies illustrated that the spiny stru ...200717449690
resistance to root-knot nematode in tomato roots expressing a nematicidal bacillus thuringiensis crystal protein.our laboratory has demonstrated previously that bacillus thuringiensis (bt) crystal (cry) proteins present in the cry5 and cry6 subclades intoxicate free-living nematodes. in this study, we tested whether the expression of nematicidal cry6a in transgenic plants provided protection against plant-parasitic nematodes. as bacterial codon usage is incompatible with expression in plants, two different codon-modified cry6a genes were synthesized for expression in plants. one was designed by maintaining ...200717451491
ecotypes of the model legume lotus japonicus vary in their interaction phenotypes with the root-knot nematode meloidogyne incognita.knowledge of host factors affecting plant-nematode interactions is scarce. here, relevant interaction phenotypes between a nodulating model host, lotus japonicus, and the endoparasitic root-knot nematode meloidogyne incognita are assessed via a genetic screen.200717475628
molecular cloning and analysis of a new venom allergen-like protein gene from the root-knot nematode meloidogyne incognita.a new venom allergen-like protein gene isolated from meloidogyne incognita (designated mi-vap-2) was cloned and analysed. the genomic clone of mi-vap-2 is 1917-bp long, contains three introns, which range in size from 39 to 797 bp, and four exons ranging in size from 37 to 361 bp. the cdna of mi-vap-2 contains an open reading frame encoding 294 amino acids, being the first 16 residues a putative secretion signal. southern blot analysis suggested that mi-vp-2 is probably a member of a small multi ...200717481609
synthesis, characterization, and biotoxicity of n n donor sulphonamide imine silicon(iv) complexes.the organosilicon derivatives of 2-[1-(2-furayl)ethyledene]sulphathiazole with organosilicon chlorides have been synthesised and characterized on the basis of analytical, conductance, and spectroscopic techniques. probable trigonal bipyramidal and octahedral structures for the resulting derivatives have been proposed on the basis of electronic, ir, (1)h, (13)c nmr, and (29)si nmr spectral studies. in the search for better fungicides, bactericides, nematicides, and insecticides studies were condu ...200617496998
soil amendment with dried weed leaves as non-chemical approach for the management of meloidogyne incognita infecting tomato.in pot trial, dried ground weed leaves of cynodon dactylon, datura stramonium, eichhomia crassipes, emex spinosus, ricinus communis and sisymbrium irio were mixed with soil at the rate of 1, 3, 5 and 10 g/kg soil and compared their nematicidal potential with carbofuran as a standard against the root-knot nematode, m. incognita infecting tomato. in addition, their effects on growth rate of tomato plants were also investigated. the results showed that m. incognita populations in the soil and root ...200617612348
nematicidal and larvicidal activities of the essential oils from aerial parts of pectis oligocephala and pectis apodocephala baker.the chemical composition of the essential oils from aerial parts of pectis apodocephala and pectis oligocephala were analyzed by gc-ms. the essential oils of these species were predominantly constituted by monoterpenes. geranial (42.9-44.5%), neral (32.2-34.2%) and alpha-pinene (10.7-11.4%) were the main constituents in the oil of p. apodocephala, while p-cymene (50.3-70.9%) and thymol (24.4-44.7%), were the prevalent compounds in the oil of p. oligocephala. the essential oils were tested agains ...200717625675
cloning of the gene lecanicillium psalliotae chitinase lpchi1 and identification of its potential role in the biocontrol of root-knot nematode meloidogyne incognita.the nematophagous fungus lecanicillium psalliotae (syn. verticillium psalliotae) is a well-known biocontrol agent. in this study, a chitinase gene lpchi1 was isolated for the first time from l. psalliotae using degenerate primers and dna-walking technique. the cloned gene lpchi1 encoding 423 amino acid residues shares a high degree of homology with other pathogenicity-related chitinases from entomopathogenic and mycoparasitic fungi. the complementary dna sequence of the mature chitinase was ampl ...200717665191
transcriptome analysis of root-knot nematode functions induced in the early stages of parasitism.root-knot nematodes of the genus meloidogyne are obligate biotrophic parasites able to infest > 2000 plant species. the nematode effectors responsible for disease development are involved in the adaptation of the parasite to its host environment and host response modulation. here, the differences between the transcriptomes of preparasitic exophytic second-stage juveniles (j2) and parasitic endophytic third-stage juveniles (j3) of meloidogyne incognita were investigated. genes up-regulated at the ...200717692078
a plant growth promoting rhizobacterium, paenibacillus polymyxa strain gbr-1, suppresses root-knot nematode.exposure of root-knot nematode, meloidogyne incognita to various concentrations (5-100%) of culture filtrate of paenibacillus polymyxa gbr-1 under in vitro conditions significantly reduced egg hatch and caused substantial mortality of its juveniles. the increase in the exposure durations of juveniles to culture filtrate and its concentrations increased the mortality rate. similarly, higher concentrations increased its inhibitory effect on egg hatch. in higher concentrations (25-100%) egg hatch w ...200817706411
nematicidal prenylated flavanones from phyllanthus niruri.two prenylated flavanones have been isolated from the hexane extract of phyllanthus niruri plant. the structure of these flavanones were established as 8-(3-methyl-but-2-enyl)-2-phenyl chroman-4-one (1) and 2-(4-hydroxyphenyl)-8-(3-methyl-but-2-enyl)-chroman-4-one (2) on the basis of spectral analysis. these were evaluated for nematicidal activity against root-knot, meloidogyne incognita, and reniform, rotylenchulus reniformis, nematodes. compound 2 exhibited nematicidal activity at par with the ...200817904597
low amounts of herbivory by root-knot nematodes affect microbial community dynamics and carbon allocation in the rhizosphere.increased carbon translocation to the rhizosphere via 'leakage' induced by low amounts of plant parasitic nematodes can foster microorganisms. the effects of the root-knot nematode meloidogyne incognita on microbial biomass (c(mic)) and community structure (phospholipid fatty acids) in the rhizosphere of barley were studied. inoculation densities of 2000, 4000, and 8000 nematodes were well below the threshold level for plant damage. a (13)co(2) pulse-labelling was performed to assess the distrib ...200717916076
a transgressive segregation factor (rkn2) in gossypium barbadense for nematode resistance clusters with gene rkn1 in g. hirsutum.host plant resistance is an important strategy for managing root-knot nematode (meloidogyne incognita) in cotton (gossypium l.). here we report evidence for enhanced resistance in interspecific crosses resulting from transgressive segregation of clustered gene loci. recently, a major gene, rkn1, on chromosome 11 for resistance to m. incognita in cv. acala nemx was identified using an intraspecific g. hirsutum cross with susceptible cv. acala sj-2. using interspecific crosses of acala nemxxsuscep ...200817940800
management of disease complex caused by root knot nematode and root wilt fungus on pigeonpea through soil organically enriched with vesicular arbuscular mycorrhiza, karanj (pongamia pinnata) oilseed cake and farmyard manure.this investigation was undertaken to compare the percentage response of colonization and development of va-mycorrhiza (glomus fasciculatum) on a number of pulse crops viz. cowpea, chickpea, soybean, pigeonpea and lentil under glasshouse conditions. among the above-mentioned crops, pigeonpea exhibited the best performance and was selected for further studies. in this host the development and colonization percentage of g. fasciculatum was investigated under two separate substrates i. e. soil amend ...200717978958
responses of heterodera glycines and meloidogyne incognita to exogenously applied neuromodulators.biogenic amines regulate important behaviours in nematodes and are associated with pharyngeal activity in plant-parasitic nematodes. a robust behavioural assay based upon nematode body movements was developed to expand the study of these and other neuroregulators in plant-parasitic nematodes. dopamine, octopamine and serotonin each had significant but differing effects on the behaviour of soybean cyst nematode heterodera glycines and root-knot nematode meloidogyne incognita juveniles. body movem ...200718005465
the involvement of phenylalanine ammonia-lyase and salicylic acid in the induction of resistance of tomato plants infested with gall nematode meloidogyne incognita. 201618047025
maize 9-lipoxygenase zmlox3 controls development, root-specific expression of defense genes, and resistance to root-knot nematodes.root-knot nematodes (rkn) are severe pests of maize. although lipoxygenase (lox) pathways and their oxylipin products have been implicated in plant-nematode interactions, prior to this report there was no conclusive genetic evidence for the function of any plant lox gene in such interactions. we showed that expression of a maize 9-lox gene, zmlox3, increased steadily and peaked at 7 days after inoculation with meloidogyne incognita rkn. mu-insertional lox3-4 mutants displayed increased attractiv ...200818052887
dose-response effects of clove oil from syzygium aromaticum on the root-knot nematode meloidogyne incognita.clove oil, derived from the plant syzygium aromaticum (l.) merr. & perry, is active against various organisms, and was prepared in a soy lecithin/detergent formulation to determine concentrations active against the root-knot nematode meloidogyne incognita (kofoid and white) chitwood.200818080287
inter- and intra-specific cuticle variation between amphimictic and parthenogenetic species of root-knot nematode (meloidogyne spp.) as revealed by a bacterial parasite (pasteuria penetrans).specific host-parasite interactions exist between species and strains of plant parasitic root-knot nematodes and the gram-positive bacterial hyperparasite pasteuria penetrans. this bacterium produces endospores that adhere to the cuticle of migrating juveniles, germinate and colonise the developing female within roots. endospore attachment of p. penetrans populations to second-stage juveniles of the root-knot nematode species meloidogyne incognita and meloidogyne hapla showed there were interact ...200818171577
effect of application timing and method on efficacy and phytotoxicity of 1,3-d, chloropicrin and metam-sodium combinations in squash plasticulture.metam-sodium, 1,3-dichloropropene (1,3-d) and chloropicrin are widely used soil fumigants. combined application of metam-sodium and 1,3-d + chloropicrin is intended to improve efficacy and broaden spectrum of control, but little is known about the effect on crop safety. this study aimed to evaluate the effects of application timing of fumigant combinations on soilborne pest and disease control (nematodes, soil fungi and weeds) and growth of squash. two separate tests with chisel-injected and dri ...200818181144
response of five lettuce cultivars to root-knot nematode, meloidogyne incognita.the root-knot nematode, meloidogyne incognito (kofoid et white) chitwood is an important pathogen of vegetables. five commercial cultivars of lettuce (lactuca sativa l.) were evaluated under greenhouse conditions for resistance to meloidogyne incognita, benguet population. plants were inoculated with 1000 eggs collected from 'apollo' tomato (lycopersicon esculentum) roots. the degree of galling and number of egg masses were assessed 4 and 8 weeks after inoculation. host plant response was classi ...200718399501
evaluation of soil biodesinfestation with crop and garden residues in the control of root-knot nematodes populations.fresh crop and garden residues were applied both under laboratory conditions and in commercial greenhouse in order to asses their effect on soil nematodes populations and soil fertility. in the laboratory experiments, dosages of 5 to 20 g of cabbage residues, chicken manure, cabbage residues+chicken manure, grass+chicken manure, as well as leaves and stems of orange tree, pine tree, oleander, olive tree, palm tree and boxwood were mixed with 500 g soil having root-knot nematodes (meloidogyne inc ...200718399508
nematicidal activity of anion transport blockers against meloidogyne incognita, caenorhabditis elegans and heterorhabditis bacteriophora.because methyl bromide has been phased out as a soil sterilant, new nematicides are urgently needed. four different chemical classes of organic acids acting as anion transport (at) blockers were tested against a free-living nematode, caenorhabditis elegans maupas, a plant-parasitic nematode, meloidogyne incognita (kofoid and white) chitwood, and an entomopathogenic nematode, heterorhabditis bacteriophora poinar, in toxicity bioassays. the materials tested were dids (4,4'-diisothiocyanatostilbene ...200818407564
[molecular cloning and characterization of an acetylcholinesterase gene dd-ace-2 from sweet potato stem nematode ditylenchus destructor].a cdna, named dd-ace-2, encoding an acetylcholinesterase (ache, ec3.1.1.7), was isolated from sweet-potato-stem nematode, ditylenchus destructor. the nucleotide and amino acid sequences among different nematode species were compared and analyzed with dnaman5.0, mega3.0 softwares. the results showed that the complete nucleotide sequence of dd-ace-2 gene of ditylenchus destructor contains 2425 base pairs from which deduced 734 amino acids (genbank accession no. ef583058). the homology rates of ami ...200818464607
potential of olive mill waste and compost as biobased pesticides against weeds, fungi, and nematodes.the phytotoxic and antimicrobial properties of olive mill wastes have been widely investigated and demonstrated over the past decade. however, their potential utilization as biodegradable pesticides against plant pathogens is still poorly understood. in this study, a series of laboratory bioassays was designed to test the inhibitory effects of sterile water extracts of two-phase olive mill waste (tpomw) and tpomw composts with different degrees of stabilization on several different plant pathoge ...200818471866
atcdka;1 silencing in arabidopsis thaliana reduces reproduction of sedentary plant-parasitic nematodes.the activity of the arabidopsis thaliana cyclin-dependent kinase atcdka;1 is important throughout g(1)/s and g(2)/m transitions and guarantees the progression of the cell cycle. inhibitor studies have shown that activation of the cell cycle is important for the development of nematode feeding sites. the aim of this study was to silence the expression of the atcdka;1 gene in nematode feeding sites to interfere with their development. therefore, sense and antisense constructs were made for the atc ...200818554267
genome sequence of the metazoan plant-parasitic nematode meloidogyne incognita.plant-parasitic nematodes are major agricultural pests worldwide and novel approaches to control them are sorely needed. we report the draft genome sequence of the root-knot nematode meloidogyne incognita, a biotrophic parasite of many crops, including tomato, cotton and coffee. most of the assembled sequence of this asexually reproducing nematode, totaling 86 mb, exists in pairs of homologous but divergent segments. this suggests that ancient allelic regions in m. incognita are evolving toward ...200818660804
evaluation of the effect of ecologic on root knot nematode, meloidogyne incognita, and tomato plant, lycopersicon esculenum.nonchemical methods and strategies for nematode management including cultural methods and engineered measures have been recommended as an alternative to methyl bromide (a major soil fumigant), due to its role in the depletion of the ozone layer. hence, an international agreement has recently been reached calling for its reduced consumption and complete phasing out. this present research evaluates the potential of ecologic, a biological, marine shell meal chitin material, as a soil amendment mana ...200818678924
papain-like cysteine proteases: key players at molecular battlefields employed by both plants and their invaders.papain-like cysteine proteases (plcps) play crucial roles in plant-pathogen/pest interactions. during these parasitic interactions, plcps act on non-self substrates, provoking the selection of counteracting inhibitors and other means to evade proteolysis. we review examples of plcps acting on molecular battlefields in the extracellular space, plant cytoplasm and herbivore gut. examples are maize mir1 (maize inbred resistance 1), tomato rcr3 (required for cladosporium resistance-3), pseudomonas a ...200818705889
plant-herbivore-carnivore interactions in cotton, gossypium hirsutum: linking belowground and aboveground.most studies on plant-herbivore interactions focus on either root or shoot herbivory in isolation, but above- and belowground herbivores may interact on a shared host plant. cotton (gossypium spp.) produces gossypol and a variety of other gossypol-like terpenoids that exhibit toxicity to a wide range of herbivores and pathogens. cotton plants also can emit herbivore-induced volatile compounds at the site of damage and systemically on all tissues above the site of damage. as these volatile compou ...200818709506
differential vascularization of nematode-induced feeding sites.sedentary nematodes are destructive plant pathogens that cause significant yield losses. in the roots of their host plants, cyst nematodes (cns) and root-knot nematodes (rkns) induce different, highly specialized feeding sites--syncytia or giant cells (gcs), respectively--to optimize nutrient uptake. we compared the mechanisms by which nutrients are delivered from the model host plant, arabidopsis, to gcs induced by the rkn meloidogyne incognita or to syncytia induced by the cn heterodera schach ...200818711135
an easy method using glutaraldehyde-introduced fluorescence for the microscopic analysis of plant biotrophic interactions.the method introduced in this article makes use of the glutaraldehyde-induced auto-fluorescence of proteins after cross-linking with glutaraldehyde for the analysis of cellular and sub-cellular structures. because the interface of biotrophic interactions is rich in proteins, the method presented is particularly suitable for the analysis of such interactions; we have exemplified its usefulness by analyzing (1) the root feeding sites induced in roots from arabidopsis thaliana by the root-knot nema ...200818778431
effects of plant vascular architecture on aboveground-belowground-induced responses to foliar and root herbivores on nicotiana tabacum.herbivores induce systemic changes in plant traits, and the strength of these induced responses is often associated with the degree of vascular connectivity that links damaged and undamaged plant tissues. although this phenomenon is known to occur aboveground in leaves, it is unknown whether or not leaf-root induction similarly follows the vascular architecture of plants. to test for this possibility, we manipulated foliar and root herbivory on tobacco (nicotiana tabacum) by the leaf-chewing ins ...200818810552
plant-nematode interactions: environmental signals detected by the nematode's chemosensory organs control changes in the surface cuticle and behaviour.plant parasitic nematodes have developed the capacity to sense and respond to chemical signals of host origin and the ability to orientate towards plant roots enhances the nematode's chance of survival. root exudates contain a range of compounds which mediate belowground interactions with pathogenic and beneficial soil organisms. chemical components of root exudates may deter one organism while attracting another and these compounds alter nematode behaviour and can either attract nematodes to th ...200818814700
pentacyclic triterpenoids from the aerial parts of lantana camara and their nematicidal activity.two new olean-12-ene triterpenoids, camarolic acid (1) and lantrigloylic acid (2), have been isolated from the aerial parts of lantana camara, along with ten known triterpenes, namely, camaric acid, lantanolic acid, lantanilic acid, pomolic acid, camarinic acid, lantoic acid, camarin, lantacin, camarinin, and ursolic acid. the new compounds have been characterized as 3,25-epoxy-3alpha-hydroxy-22beta-{[(s)-3-hydroxy-2-methylidenebutanoyl]oxy}olean-12-en-28-oic acid (1) and 3,25-epoxy-3alpha-hydro ...200818816515
host plant response of burrowing nematode resistant musa genotypes to the root-knot nematode, meloidogyne incognita. 200818831278
molecular detection of nematicidal crystalliferous bacillus thuringiensis strains of iran and evaluation of their toxicity on free-living and plant-parasitic nematodes.the characterization of nematode-effective strains and cry genes in the iranian bacillus thuringiensis (bt) collection (70 isolates) is presented. characterization was based on pcr analysis using 12 specific primers for cry5, cry6, cry12, cry13, cry14, and cry21 genes encoding proteins active against nematodes, crystal morphology, and protein band patterns as well as their nematicidal activity on root-knot nematode (meloidogyne incognita) and two free-living nematodes (chiloplacus tenuis and acr ...200818923549
purification and characterization of chitinases from paecilomyces variotii dg-3 parasitizing on meloidogyne incognita eggs.two extracellular chitinases were purified from paecilomyces variotii dg-3, a chitinase producer and a nematode egg-parasitic fungus, to homogeneity by deae sephadex a-50 and sephadex g-100 chromatography. the purified enzymes were a monomer with an apparent molecular mass of 32 kda (chi32) and 46 kda (chi46), respectively, and showed chitinase activity bands with 0.01% glycol chitin as a substrate after sds-page. the first 20 and 15 n-terminal amino acid sequences of chi32 and chi46 were determ ...200918936994
potentiality of different isolates of wilt fungus fusarium oxysporum collected from rhizosphere of tomato against root-knot nematode meloidogyne incognita.this investigation was undertaken to determine the effect of culture filtrates of different isolates of fusarium oxysporum f. sp. lycopersici on mortality of meloidogyne incognita juveniles and egg hatching. it was observed that different concentrations including standard extract (s.e), 1:10 and 1:100 dilutions of all fungal filtrates inhibited egg hatch when compared with control. minimum mortality and maximum hatching was observed in brt (showing least mortality) isolate of f. oxysporum, while ...200818941992
a fusarium wilt resistance gene in gossypium barbadense and its effect on root-knot nematode-wilt disease complex.abstract fusarium wilt, caused by the soilborne pathogen fusarium oxysporum f. sp. vasinfectum race 1, is a vascular disease in cotton (gossypium spp.), and is a component of a disease complex with root-knot nematodes (meloidogyne incognita). genetic analysis of two interspecific crosses (g. barbadense pima s-7 x g. hirsutum acala nemx and pima s-7 x acala sj-2) showed that one major gene (designated fov1) with allele dosage effect conferred resistance to f. oxysporum f. sp. vasinfectum race 1 i ...200618943146
activity of hydroxamic acids from secale cereale against the plant-parasitic nematodes meloidogyne incognita and xiphinema americanum.abstract cyclic hydroxamic acids are secondary metabolites found in the family poaceae and have been implicated in the allelopathy of rye (secale cereale). the toxicity of these compounds against plant-parasitic nematodes is unknown. diboa (2,4-dihydroxy-(2h)-1,4-benzoxazin-3(4h)-one), dimboa (2,4-hydroxy-7-methoxy-(2h)-1,4-benzoxazin-3(4h)-one), and their degradation products boa (benzoxazolin-2(3h)-one) and mboa (6-methoxy-benzoxazolin-2(3h)-one) were screened in vitro against meloidogyne inco ...200518943462
a molecular marker correlated with selected virulence against the tomato resistance gene mi in meloidogyne incognita, m. javanica, and m. arenaria.abstract root-knot nematodes of the genus meloidogyne are economically important pathogens of a wide range of crops. the tomato resistance gene mi typically confers resistance to the three major species, m. incognita, m. javanica, and m. arenaria. however, virulent populations completely overcoming the mi resistance still occur. in an attempt to develop molecular markers for virulence against mi and gain insights into the genetic relationships among virulent populations of different species and ...200118943850
endophytic colonization of plants by the biocontrol agent rhizobium etli g12 in relation to meloidogyne incognita infection.abstract the external and internal colonization of potato and arabidopsis roots by the biocontrol strain rhizobium etli g12 containing a plasmidborne trp promoter green fluorescent protein transcriptional fusion, pgt-trp, was studied in the presence and absence of the root-knot nematode meloidogyne incognita. plant colonization behavior and biocontrol potential of the marked strain g12(pgt-trp) was not altered compared with the parental strain. plasmid pgt-trp was stable for more than 80 generat ...200118943855
does the presence of detached root border cells of zea mays alter the activity of the pathogenic nematode meloidogyne incognita?abstract the root-knot nematode meloidogyne incognita is a major pathogen of a range of important crops. currently, control is typically achieved by the use of nematicides. however, recent work suggests that manipulating the ability of roots to slough off border cells, which then act as a decoy to the nematode, can significantly decrease damage to the roots. we investigated the attractiveness of border cells to m. incognita and the response of the nematode to border cells in close proximity. we ...200318944094
species-dependent effects of border cell and root tip exudates on nematode behavior.abstract effects of border cell and root tip exudates on root knot nematode (meloidogyne incognita) behavior were examined. in whole-plant assays using pea, m. incognita second-stage juveniles (j2) accumulated rapidly around the 1- to 2-mm apical region ensheathed by border cells, but not in the region of elongation. within 15 to 30 min, j2 which had accumulated within detached clumps of border cells lost motility and entered into a quiescent state. when border cells (and associated root tip exu ...200018944426
effect of temperature on and histopathology of the interaction between meloidogyne incognita and thielaviopsis basicola on cotton.abstract controlled environments were used to study the relationship between the root-knot nematode (meloidogyne incognita) and thielaviopsis basicola on cotton. temperature treatments were continuous 20, 24, and 28 degrees c or two cyclic linear regimes with ranges of 14 to 32 or 18 to 28 degrees c over 24 h. cotton seeds were planted in fumigated soil infested with t. basicola, m. incognita, or both. after 42 days, pathogen effects on plant growth and pathogen development were evaluated. histo ...199918944671
inoculum densities of fusarium oxysporum f. sp. vasinfectum and meloidogyne incognita in relation to the development of fusarium wilt and the phenology of cotton plants (gossypium hirsutum).abstract development of fusarium wilt in upland cotton (gossypium hirsutum) usually requires infections of plants by both meloidogyne incognita and fusarium oxysporum f. sp. vasinfectum. in this study, the soil densities of m. incognita and f. oxysporum f. sp. vasinfectum and the incidence of fusarium wilt in three field sites were determined in 1982-1984. multiple regression analysis of percent incidence of fusarium wilt symptoms on population densities of m. incognita and f. oxysporum f. sp. v ...199718945178
meloidogyne incognita: molecular cloning and characterization of a cdna encoding a cathepsin d-like aspartic proteinase.herein we describe the cloning and characterization of a cdna encoding an aspartic proteinase from the root-knot nematode meloidogyne incognita. using pcr techniques, a 1471-bp cdna fragment encoding a cathepsin d-like (mi-asp1) transcript was isolated from second-stage larvae mrna. its predicted amino acid sequence comprises a pro-region of 71 amino acid residues and a mature protease of 378 amino acid residues with a predicted molecular mass of 41.502kda. protein sequence comparisons of mi-asp ...200918952081
direct identification of the meloidogyne incognita secretome reveals proteins with host cell reprogramming potential.the root knot nematode, meloidogyne incognita, is an obligate parasite that causes significant damage to a broad range of host plants. infection is associated with secretion of proteins surrounded by proliferating cells. many parasites are known to secrete effectors that interfere with plant innate immunity, enabling infection to occur; they can also release pathogen-associated molecular patterns (pamps, e.g., flagellin) that trigger basal immunity through the nematode stylet into the plant cell ...200818974830
effect of a formulation of bacillus firmus on root-knot nematode meloidogyne incognita infestation and the growth of tomato plants in the greenhouse and nursery.bacillus firmus, commercial wp formulation (bionem) was evaluated against the root-knot nematode meloidogyne incognita in a laboratory, greenhouse and under field conditions on tomato plants. in the laboratory tests, an aqueous suspension of bionem at 0.5%, 1%, 1.5% and 2% concentration reduced egg hatching from 98% to 100%, 24-days after treatment. treatment of second-stage juveniles with 2.5% and 3% concentration of bionem, caused 100% inhibition of mobility, 24 h after treatment. in the green ...200919041655
larvicidal and nematicidal activities of the leaf essential oil of croton regelianus.the chemical composition of the leaf essential oil of croton regelianus collected from wild plants growing in two different sites at ceará state (brazil) was analyzed by gc/ms and gc-fid. twenty monoterpenoids, representing more than 96% of the chemical composition of the oils, were identified and quantified. the oils showed similar chemical composition but considerable variation in the levels of each constituent. ascaridole (33.9-17.0%), p-cymene (22.3-21.6%), and camphor (13.0-3.1%) were the p ...200819089831
analysis of cotton (gossypium hirsutum) root proteomes during a compatible interaction with the black root rot fungus thielaviopsis basicola.a proteomic approach was used to uncover the inducible molecular defense mechanism of cotton root occurring during the compatible interaction with thielaviopsis basicola. microscopic observation of cotton root inoculated with a suspension of conidia showed that this necrotrophic hemibiotroph fungus interacts with the plant and completes its life cycle in our experimental system. 2-de analysis of root extracts taken after 1, 3, 5, and 7 days postinoculation and cluster analysis of the protein exp ...200919105169
biocontrol of root-knot nematode, meloidogyne incognita damaging queen palm, livistona rotundifolia using trichoderma species.livistona rotundifolia is a widely grown queen palm in all the net houses of floriculture industries in sri lanka. it is grown to an extent of 10,000 mz in green farms ltd, marawilla under shade net house. root knot nematode is one among the key pests of queen palms and causes heavy loss in the queen palm industry. queen palm is grown in a coir based compost media under sprinkler irrigation. management of nematodes using chemicals is impractical due the non availability and selectivity of the ne ...200819226812
decomposers and root feeders interactively affect plant defence in sinapis alba.aboveground herbivory is well known to change plant growth and defence. in contrast, effects of soil organisms, acting alone or in concert, on allocation patterns are less well understood. we investigated separate and combined effects of the endogeic earthworm species aporrectodea caliginosa and the root feeding nematode species pratylenchus penetrans and meloidogyne incognita on plant responses including growth and defence metabolite concentrations in leaves of white mustard, sinapis alba. soil ...200919252930
root-knot nematode management in double-cropped plasticulture vegetables.combination treatments of chisel-injected fumigants (methyl bromide, 1,3-d, metam sodium, and chloropicrin) on a first crop, followed by drip-applied fumigants (metam sodium and 1,3-d +/- chloropicrin) on a second crop, with and without oxamyl drip applications were evaluated for control of meloidogyne incognita in three different tests (2002 to 2004) in tifton, ga. first crops were eggplant or tomato, and second crops were cantaloupe, squash, or jalapeno pepper. double-cropped vegetables suffer ...200619259431
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