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evaluation of steam and soil solarization for meloidogyne arenaria control in florida floriculture crops.steam and soil solarization were investigated for control of the root-knot nematode meloidogyne arenaria in 2 yr of field trials on a commercial flower farm in florida. the objective was to determine if preplant steam treatments in combination with solarization, or solarization alone effectively controlled nematodes compared to methyl bromide (mebr). trials were conducted in a field with naturally occurring populations of m. arenaria. treatments were solarization alone, steam treatment after sol ...201627765992
reproduction of meloidogyne incognita race 3 on flue-cured tobacco homozygous for rk1 and/or rk2 resistance genes.most commercial tobacco cultivars possess the rk1 resistance gene to races 1 and 3 of meloidogyne incognita and race 1 of meloidogyne arenaria, which has caused a shift in population prevalence in virginia tobacco fields toward other species and races. a number of cultivars now also possess the rk2 gene for root-knot resistance. experiments were conducted in 2013 to 2014 to examine whether possessing both rk1 and rk2 increases resistance to a variant of m. incognita race 3 compared to either gen ...201627418700
root transcriptome analysis of wild peanut reveals candidate genes for nematode resistance.wild peanut relatives (arachis spp.) are genetically diverse and were adapted to a range of environments during the evolution course, constituting an important source of allele diversity for resistance to biotic and abiotic stresses. the wild diploid a. stenosperma harbors high levels of resistance to a variety of pathogens, including the root-knot nematode (rkn) meloidogyne arenaria, through the onset of the hypersensitive response (hr). in order to identify genes and regulators triggering this ...201526488731
suitability of zucchini and cucumber genotypes to populations of meloidogyne arenaria, m. incognita, and m. javanica.the host suitability of five zucchini and three cucumber genotypes to meloidogyne incognita (mipm26) and m. javanica (mj05) was determined in pot experiments in a greenhouse. the number of egg masses (em) did not differ among the genotypes of zucchini or cucumber, but the eggs/plant and reproduction factor (rf) did slightly. m. incognita mipm26 showed lower em, eggs/plant, and rf than m. javanica mj05. examination of the zucchini galls for nematode postinfection development revealed unsuitable c ...201525861120
broad meloidogyne resistance in potato based on rna interference of effector gene 16d10.root-knot nematodes (meloidogyne spp.) are a significant problem in potato (solanum tuberosum) production. there is no potato cultivar with meloidogyne resistance, even though resistance genes have been identified in wild potato species and were introgressed into breeding lines. the objectives of this study were to generate stable transgenic potato lines in a cv. russet burbank background that carry an rna interference (rnai) transgene capable of silencing the 16d10 meloidogyne effector gene, an ...201525861119
mitochondrial genome plasticity among species of the nematode genus meloidogyne (nematoda: tylenchina).the mitochondrial (mt) genomes of the plant-parasitic root-knot nematodes meloidogyne arenaria, meloidogyne enterolobii and meloidogyne javanica were sequenced and compared with those of three other root-knot nematode species in order to explore the mt genome plasticity within meloidogyne. the mt genomes of m. arenaria, m. enterolobii and m. javanica are circular, with an estimated size of 18.8, 18.9 and 19.6 kb, respectively. compared to other nematodes these mt genomes are larger, due to the p ...201525655462
genetic mapping of resistance to meloidogyne arenaria in arachis stenosperma: a new source of nematode resistance for peanut.root-knot nematodes (rkn; meloidogyne sp.) are a major threat to crops in tropical and subtropical regions worldwide. the use of resistant crop varieties is the preferred method of control because nematicides are expensive, and hazardous to humans and the environment. peanut (arachis hypogaea) is infected by four species of rkn, the most damaging being m. arenaria, and commercial cultivars rely on a single source of resistance. in this study, we genetically characterize rkn resistance of the wil ...201526656152
pasteuria penetrans for control of meloidogyne incognita on tomato and cucumber, and m. arenaria on snapdragon.meloidogyne incognita and meloidogyne arenaria are important parasitic nematodes of vegetable and ornamental crops. microplot and greenhouse experiments were conducted to test commercial formulations of the biocontrol agent pasteuria penetrans for control of m. incognita on tomato and cucumber and m. arenaria on snapdragon. three methods of application for p. penetrans were assessed including seed, transplant, and post-plant treatments. efficacy in controlling galling and reproduction of the two ...201526527842
evaluation of cover crops with potential for use in anaerobic soil disinfestation (asd) for susceptibility to three species of meloidogyne.several cover crops with potential for use in tropical and subtropical regions were assessed for susceptibility to three common species of root-knot nematode, meloidogyne arenaria, m. incognita, and m. javanica. crops were selected based on potential use as organic amendments in anaerobic soil disinfestation (asd) applications. nematode juvenile (j2) numbers in soil and roots, egg production, and host plant root galling were evaluated on arugula (eruca sativa, cv. nemat), cowpea (vigna unguicula ...201324379486
characterization of a root-knot nematode population of meloidogyne arenaria from tupungato (mendoza, argentina).root-knot nematodes (meloidogyne spp.) are polyphagous plant parasites of global importance. successful host infection depends on the particular interaction between a specific nematode species and race and a specific plant species and cultivar. accurate diagnosis of nematode species is relevant to effective agricultural management; and benefits further from understanding the variability within a single nematode species. here, we described a population of m. arenaria race 2 from mendoza (argentin ...201223481918
rkn lethal db: a database for the identification of root knot nematode (meloidogyne spp.) candidate lethal genes.root knot nematode (rkn; meloidogyne spp.) is one of the most devastating parasites that infect the roots of hundreds of plant species. rkn cannot live independently from their hosts and are the biggest contributors to the loss of the world's primary foods. rnai gene silencing studies have demonstrated that there are fewer galls and galls are smaller when rnai constructs targeted to silence certain rkn genes are expressed in plant roots. we conducted a comparative genomics analysis, comparing rk ...201223144556
susceptibility of several common subtropical weeds to meloidogyne arenaria, m. incognita, and m. javanica.experiments were conducted in the greenhouse to assess root galling and egg production of three root-knot nematode species, meloidogyne arenaria, m. incognita, and m. javanica, on several weeds common to florida agricultural land. weeds evaluated were amaranthus retroflexus (redroot pigweed), cyperus esculentus (yellow nutsedge), eleusine indica (goosegrass), portulaca oleracea (common purslane), and solanum americanum (american black nightshade). additionally, although it is recommended as a co ...201223482324
differential gene expression in roots of nematode-resistant and -susceptible peanut (arachis hypogaea) cultivars in response to early stages of peanut root-knot nematode (meloidogyne arenaria) parasitization.the peanut root-knot nematode (rkn, meloidogyne arenaria) can cause significant yield losses in cultivated peanut (arachis hypogaea). however, molecular events underlying successful rkn infection and host responses in peanut are sparsely understood. using suppression subtractive hybridization (ssh), cdna libraries, enriched with differentially expressed ests, were constructed from rkn-challenged root tissues in the pre-penetration and early infection stages from near-isogenic nematode-resistant ...201120863592
potential of leguminous cover crops in management of a mixed population of root-knot nematodes (meloidogyne spp.).root-knot nematode is an important pest in agricultural production worldwide. crop rotation is the only management strategy in some production systems, especially for resource poor farmers in developing countries. a series of experiments was conducted in the laboratory with several leguminous cover crops to investigate their potential for managing a mixture of root-knot nematodes (meloidogyne arenaria, m. incognita, m. javanica). the root-knot nematode mixture failed to multiply on mucuna prurie ...201022736854
host status of endophyte-infected and noninfected tall fescue grass to meloidogyne spp.tall fescue grass cultivars with or without endophytes were evaluated for their susceptibility to meloidogyne incognita in the greenhouse. tall fescue cultivars evaluated included, i) wild-type jesup (e+, ergot-producing endophyte present), ii) endophyte-free jesup (e-, no endophyte present), iii) jesup (max-q, non-ergot producing endophyte) and iv) georgia 5 (e+). peach was included as the control. peach supported greater (p ≤ 0.05) reproduction of m. incognita than all tall fescue cultivars. d ...201022736851
effects of midas® on nematodes in commercial floriculture production in florida.cut flower producers currently have limited options for nematode control. four field trials were conducted in 2006 and 2007 to evaluate midas® (iodomethane:chloropicrin 50:50) for control of root-knot nematodes (meloidogyne arenaria) on celosia argentea var. cristata in a commercial floriculture production field in southeastern florida. midas (224 kg/ha) was compared to methyl bromide:chloropicrin (98:2, 224 kg/ha), and an untreated control. treatments were evaluated for effects on meloidogyne a ...201022736832
coprinus comatus: a basidiomycete fungus forms novel spiny structures and infects nematode.nematophagous basidiomycete fungi kill nematodes by trapping, endoparasitizing and producing toxin. in our studies coprinus comatus (o.f.müll. : fr.) pers. is found to be a nematode-destroying fungus; this fungus immobilizes, kills and uses free-living nematode panagrellus redivivus goodey and root-knot nematode meloidogyne arenaria neal. c. comatus produces an unusual structure designated spiny ball. set on a sporophore-like branch, the spiny ball is a burr-like structure assembled with a large ...201021148944
evaluation of an antibiotic-producing strain of pseudomonas fluorescens for suppression of plant-parasitic nematodes.the antibiotic 2,4-diacetylphloroglucinol (dapg), produced by some strains of pseudomonas spp., is involved in suppression of several fungal root pathogens as well as plant-parasitic nematodes. the primary objective of this study was to determine whether wood1r, a d-genotype strain of dapg-producing p. fluorescens, suppresses numbers of both sedentary and migratory plant-parasitic nematodes. an experiment was conducted in steam-heated soil and included two seed treatments (with wood1r and a cont ...200922736820
population dynamics of meloidogyne arenaria and pasteuria penetrans in a long-term crop rotation study.the endospore-forming bacterium pasteuria penetrans is an obligate parasite of root-knot nematodes (meloidogyne spp.). the primary objective of this study was to determine the effect of crop sequence on abundance of p. penetrans. the experiment was conducted from 2000 to 2008 at a field site naturally infested with both the bacterium and its host meloidogyne arenaria and included the following crop sequences: continuous peanut (arachis hypogaea) (p-p-p) and peanut rotated with either 2 years of ...200922736828
using fame analysis to compare, differentiate, and identify multiple nematode species.we have adapted the sherlock(®) microbial identification system for identification of plant parasitic nematodes based on their fatty acid profiles. fatty acid profiles of 12 separate plant parasitic nematode species have been determined using this system. additionally, separate profiles have been developed for rotylenchulus reniformis and meloidogyne incognita based on their host plant, four species and three races within the meloidogyne genus, and three life stages of heterodera glycines. stati ...200922736811
molecular and morphological characterization of an unusual meloidogyne arenaria population from traveler's tree, ravenala madagascariensis.an unusual variant of meloidogyne arenaria was discovered on roots of a traveler's tree (ravenala madagascariensis) intended for display at a public arboretum in pennsylvania. the population aroused curiosity by the lack of visible galling on the roots of the infected plant, and the female vulval region was typically surrounded by egg sacs. most morphometrics of the population fit within the ranges reported for m. arenaria, with a mosaic of features in common with either m. platani or other trop ...200819440257
comparison of methods for assessing resistance to meloidogyne arenaria in peanut.use of resistant cultivars is a desirable approach to manage the peanut root-knot nematode (meloidogyne arenaria). to incorporate resistance into commercially acceptable cultivars requires reliable, efficient screening methods. to optimize the resistance screening protocol, a series of greenhouse tests were done using seven genotypes with three levels of resistance to m. arenaria. the three resistance levels could be separated based on gall indices as early as two weeks after inoculation (wai) u ...200719259486
virulence of meloidogyne spp. and induced resistance in grape rootstocks.harmony grape rootstock displays resistance to several meloidogyne spp. but that resistance is not durable in commercial vineyard settings. a 2-year experiment in a microplot setting revealed host specificities of two virulent populations of meloidogyne arenaria and an avirulent population of meloidogyne incognita. in a subsequent split-root experiment, the avirulent nematode population was demonstrated to induce resistance to the virulent nematode population. to quantify the level of resistance ...200719259475
observations on the suppression of root-knot nematode (meloidogyne arenaria) on tomato by incorporation of cyanobacterial powder (oscillatoria chlorina) into potting field soil.experiments were carried out to investigate the nematicidal potential of a cyanobacterium, oscillatoria chlorina, against the root-knot nematode, meloidogyne arenaria on tomato plants grown in pots filled with 500 cm3 of field soil infested with 12-s stage juveniles (j2)/cm3 soil. incorporation of freeze-dried cyanobacterial powder into potted field soil at the rate of 0.2%, 0.4%, 0.6%, 0.8% and 1.0% (w/w) 5 days prior to tomato planting, reduced root galling, final population of m. arenaria and ...200716458501
root vs pod infection by root-knot nematodes on aflatoxin contamination of peanut.aflatoxins are potent carcinogens produced by some aspergillus spp. infection of peanut (arachis hypogaea) by root-knot nematodes (meloidogyne arenaria) can lead to an increase in aflatoxin contamination of kernels when the plants are subjected to drought stress during pod maturation. it is not clear whether the increased aflatoxin contamination is primarily due to greater invasion of the galled pods by toxigenic aspergillus spp. or whether root galling is also involved. our objective was to det ...200718399500
isolation and characterization of another cdna encoding a chorismate mutase from the phytoparasitic nematode meloidogyne arenaria.a new cdna, named ma-cm-2, encoding a chorismate mutase (cm), has been isolated from meloidogyne arenaria. the full-length cdna, carrying the trans-spliced sl1 leader sequence, was 753-bp long with an open reading frame of 576 bp. the deduced protein ma-cm-2 including amino-terminal signal peptide shows significant similarity to cms of meloidogyne incognita, meloidogyne javanica, and also bacteria. secondary structure prediction of ma-cm-2 indicates the presence of the three conserved alpha-heli ...200616458297
sensitive pcr detection of meloidogyne arenaria, m. incognita, and m. javanica extracted from soil.we have developed a simple pcr assay protocol for detection of the root-knot nematode (rkn) species meloidogyne arenaria, m. incognita, and m. javanica extracted from soil. nematodes are extracted from soil using baermann funnels and centrifugal flotation. the nematode-containing fraction is then digested with proteinase k, and a pcr assay is carried out with primers specific for this group of rkn and with universal primers spanning the its of rrna genes. the presence of rkn j2 can be detected a ...200619259460
preliminary study of the green algae chlorella (chlorella vulgaris) for control on the root-knot nematode (meloidogyne arenaria) in tomato plants and ectoparasite xiphinema indexin grape seedlings.the alternative ecological methods require investigation of many organo-biological means for plant protection against dangerous root parasites such as root-knot nematode meloidogyne arenaria and some ectoparasites (xiphinema index). the bulgarian organic product - dry extract of green alga chlorella vulgaris ("the golden apple"-plamen barakov) is the latest product, which in comparative aspect gives the best results. series of laboratory and pot experiments are carried out with tomato (cv. bele ...200516628939
conserved and variable domains in satellite dnas of mitotic parthenogenetic root-knot nematode species.two satellite dnas have been characterized in the mitotic parthenogenetic root-knot nematodes meloidogyne javanica and m. paranaensis, agriculturally important phytoparasitic species. the satellite repeat variants cloned from m. javanica could not be resolved from those described earlier in m. arenaria [castagnone-sereno, p., leroy, f., abad, p., 2000. cloning and characterization of an extremely conserved satellite dna family from the root-knot nematode meloidogyne arenaria. genome 43, 346-353] ...200516229973
a recessive gene for resistance to meloidogyne arenaria in interspecific arachis spp. hybrids.a single dominant gene for resistance to meloidogyne arenaria was identified previously in two peanut cultivars, arachis hypogaea 'coan' and 'nematam'. the interspecific arachis hybrid txag-6 was the source of this resistance and the donor parent in a backcross breeding program to introgress resistance into cultivated peanut. to determine if other resistance genes were present in txag-6 and derived breeding populations from the third backcross generation (bc), f individuals were evaluated for th ...200519262858
the ever-expanding neuropeptide gene families in the nematode caenorhabditis elegans.neuropeptides act as chemical signals in the nervous system to modulate behaviour. with the ongoing est projects and dna sequence determination of different genomes, the identification of neuropeptide genes has been made easier. despite the relatively 'simple' repertoire of behaviours in the nematode caenorhabditis elegans, this worm contains a surprisingly large and diverse set of neuropeptide genes. at least 109 genes encoding over 250 potential neuropeptides have been identified in c. elegans ...200516569285
morphological variability and molecular phylogeny of the nematophagous fungus monacrosporium drechsleri.an isolate of the nematode-trapping fungus monacrosporium drechsleri was collected from cultures of the root-knot nematode meloidogyne arenaria that had been maintained on tomato roots in greenhouse pots in beltsville, maryland. the plant-parasitic nematodes heterodera glycines, meloidogyne incognita and pratylenchus zeae and the free-living nematodes caenorhabditis elegans and panagrellus redivivus were placed on colonies of m. drechsleri grown in petri dishes to study ability of the isolate to ...200516396348
resolving tylenchid evolutionary relationships through multiple gene analysis derived from est data.sequence-based phylogenetic analyses typically are based on a small number of character sets and report gene trees which may not reflect the true species tree. we employed an est mining strategy to suppress such incongruencies, and recovered the most robust phylogeny for five species of plant-parasitic nematode (meloidogyne arenaria, m. chitwoodi, m. hapla, m. incognita, and m. javanica), three closely related tylenchid taxa (heterodera glycines, globodera pallida, and g. rostochiensis) and a di ...200515876542
nematicidal activity and chemical component of poria cocos.poria cocos, a famous traditional chinese medicine, was found to have nematicidal activity in experiments searching for nematicidal fungi. the experiment showed it could kill 94.9% of the saprophytic nematode, panagrellus redivivue, 92.6% of the root-knot nematode, meloidogyne arenaria, and 93.5% of the pine nematode, bursaphelenchus xylophilus, on pda plate within 12 hours. according to the nematicidal activity, three new compounds, 2, 4, 6-triacetylenic octane diacid, 2, 4, 5, 6-tetrahydroxyhe ...200515765052
distribution and downward movement of pasteuria penetrans in field soil.endospores of pasteuria penetrans were evaluated for their vertical distribution in field soil and their downward movement through soil in the laboratory. in the field trial, the number of endospores attached to second-stage juveniles (j2) of meloidogyne arenaria race 1 varied greatly in different soil depths. there were higher percentages of j2 with endospores attached in former weed fallow plots during the first 3 years of growing peanut than in former bahiagrass and rhizomal peanut plots (p < ...200519262855
persistence and suppressiveness of pasteuria penetrans to meloidogyne arenaria race.the long-term persistence and suppressiveness of pasteuria penetrans against meloidogyne arenaria race 1 were investigated in a formerly root-knot nematode suppressive site following 9 years of continuous cultivation of three treatments and 4 years of continuous peanut. the three treatments were two m. arenaria race 1 nonhost crops, bahiagrass (paspalum notatum cv. pensacola var. tifton 9), rhizomal peanut (arachis glabrata cv. florigraze), and weed fallow. two root-knot nematode susceptible wee ...200419262836
life cycle, ultrastructure, and host specificity of the north american isolate of pasteuria that parasitizes the soybean cyst nematode, heterodera glycines.light and transmission electron microscopy were used to investigate the life cycle and ultrastructure of an undescribed isolate of pasteuria that parasitizes the soybean cyst nematode, heterodera glycines. studies also were conducted to determine the host specificity of pasteuria. the endospores that attached to the cuticle of second-stage juveniles (j2) of h. glycines in soil did not germinate until the encumbered nematodes invaded soybean roots. thereafter, the bacterium developed and complete ...200419262804
morphological and molecular characterization of meloidogyne mayaguensis isolates from florida.the discovery of meloidogyne mayaguensis is confirmed in florida; this is the first report for the continental united states. meloidogyne mayaguensis is a virulent species that can reproduce on host cultivars bred for nematode resistance. the perineal patterns of m. mayaguensis isolates from florida show morphological variability and often are similar to m. incognita. useful morphological characters for the separation of m. mayaguensis from m. incognita from florida are the male stylet length va ...200419262811
location of independent root-knot nematode resistance genes in plum and peach.prunus species express different ranges and levels of resistance to the root-knot nematodes (rkn) meloidogyne spp. in myrobalan plum ( prunus cerasifera), the dominant ma gene confers a high-level and wide-spectrum resistance to the predominant rkn, meloidogyne arenaria, meloidogyne incognita, meloidogyne javanica and the isolate meloidogyne sp. florida which overcomes the resistance of the amygdalus sources. in japanese plum ( prunus salicina), a similar wide-spectrum dominant resistance gene, ...200414569426
relationship between meloidogyne arenaria and aflatoxin contamination in peanut.damaged and developing kernels of peanut (arachis hypogaea) are susceptible to colonization by fungi in the aspergillus flavus group which, under certain conditions, produces aflatoxins prior to harvest. our objective was to determine whether infection of peanut roots and pods by meloidogyne arenaria increases aflatoxin contamination of the kernels when peanut is subjected to drought stress. the experiment was a completely randomized 2-x-2 factorial with 6 replicates/treatment. the treatment fac ...200419262803
detection of pasteuria penetrans infection in meloidogyne arenaria race 1 in planta by polymerase chain reaction.we report on the development of a pcr-based assay to detect pasteuria penetrans infection of meloidogyne arenaria in planta using specific primers for recently sequenced sige, spoiiab and atpf genes of p. penetrans biotype p20. amplification of these genes in crude dna extracts of ground tomato root galls using real-time kinetic pcr distinguished infected from uninfected m. arenaria race 1 by analysis of consensus thresholds for single copy genes. fluorescent in situ hybridization (fish) using t ...200419712314
reproduction of meloidogyne spp. on resistant peanut genotypes from three breeding programs.three described species of root-knot nematode parasitize peanut (arachis hypogaea): meloidogyne arenaria race 1 (ma), m. hapla (mh), and m. javanica (mj). peanut cultivars with broad resistance to meloidogyne spp. will be useful regardless of the species present in the field. the objective of this study was to determine whether peanut genotypes with resistance to m. arenaria originating from three different breeding programs were also resistant to m. hapla and m. javanica. the experiment used a ...200319262773
evaluation of changes in the element content and biomass of invaded with meloidogyne arenaria tiny tim tomato plants under nh4vo3 treatment.the parasite-host system meloidogyne arenaria--tiny tim tomato plants has been studied in order to investigate the influence of the process of invasion on the chemical composition and biomass of plants. the concentrations of seven chemical elements cu, zn, mg, k, na, mn and fe have been determined using aas in controls and invaded plants, and their changes have been evaluated under treatment with nh4vo3 in three different concentrations 0.01, 0.1 and 0.13 mg/100 ml h2o. the process of treatment ...200314711041
meloidogyne javanica on peanut in florida.a mixed population of meloidogyne arenaria race 1 and m. javanica race 3 is reported on peanut from a field in levy county, florida. confirmation of m. javanica on peanut is based on esterase and malate dehydrogenase isozyme patterns resolved on polyacrylamide slab gels following electrophoresis, and perineal patterns. up to 29% of 290 individual females collected from peanut roots in the field in autumn 2002 showed a typical esterase j3 phenotype for m. javanica. this is the third report of m. ...200319262776
nucleotide substitution patterning within the meloidogyne rdna d3 region and its evolutionary implications.evolutionary relationships based on nucleotide variation within the d3 26s rdna region were examined among acollection of seven meloidogyne hapla isolates and seven isolates of m. arenaria, m. incognita, and m. javanica. using d3a and d3b primers, a 350-bp region was pcr amplified from genomic dna and double-stranded nucleotide sequence obtained. phylogenetic analyses using three independent clustering methods all provided support for a division between the automictic m. hapla and the apomictic ...200319262771
mechanism of resistance to meloidogyne arenaria in the peanut cultivar coan.resistance to meloidogyne arenaria in the peanut cultivar coan is inherited as a single, dominant gene. the mechanism of resistance to m. arenaria in coan was evaluated in three experiments. in the first experiment the number of second-stage juveniles (j2) of m. arenaria penetrating roots of the susceptible cultivar florunner was higher than the number of j2 penetrating roots of the resistant peanut cultivar coan (p < 0.05). in a second experiment it was determined that the root size and number ...200319265984
the heat-stable root-knot nematode resistance gene mi-9 from lycopersicon peruvianum is localized on the short arm of chromosome 6.the tomato gene mi-1 confers resistance to three species of root-knot nematodes, meloidogyne spp. however, the resistance mediated by mi-1 is inactive at soil temperatures above 28 degrees c. previously, we identified and mapped a novel heat-stable nematode resistance gene from the wild species lycopersicon peruvianum accession la2157 on to chromosome 6. here we report further characterization of this heat-stable resistance against three mi-1-avirulent biotypes of meloidogyne javanica, meloidogy ...200312589548
temporal formation and immunolocalization of an endospore surface epitope during pasteuria penetrans sporogenesis.the synthesis and localization of an endospore surface epitope associated with the development of pasteuria penetrans was determined using a monoclonal antibody (mab) as a probe. nematodes, uninfected or infected with p. penetrans, were harvested at 12, 16, 24, and 38 days after inoculation (dai) and then examined to determine the developmental stage of the bacterium. vegetative growth of p. penetrans was observed only in infected nematodes harvested at 12 and 16 dai, whereas cells at different ...200319262762
pasteuria spp.: systematics and phylogeny of these bacterial parasites of phytopathogenic nematodes.pasteuria spp. include endospore-forming bacterial pathogens of cladoceran crustaceans and plant-parasitic nematodes. propagation of these nematode pathogens requires attachment of soilborne endospores to nematode hosts, infection, growth, sporulation, and release of endospores to repeat the cycle of infection and propagation. the ability of these bacteria to suppress the levels of plant-parasitic nematodes in the field has made them particularly promising candidates for biocontrol of nematode d ...200319265995
host suitability of the olive cultivars arbequina and picual for plant-parasitic nematodes.host suitability of olive cultivars arbequina and picual to several plant-parasitic nematodes was studied under controlled conditions. arbequina and picual were not suitable hosts for the root-lesion nematodes pratylenchus fallax, p. thornei, and zygotylenchus guevarai. however, the ring nematode mesocriconema xenoplax and the spiral nematodes helicotylenchus digonicus and h. pseudorobustus reproduced on both olive cultivars. the potential of meloidogyne arenaria race 2, m. incognita race 1, and ...200319265971
effects of switchgrass (panicum virgatum) rotations with peanut (arachis hypogaea l.) on nematode populations and soil microflora.a 3-year field rotation study was conducted to assess the potential of switchgrass (panicum virgatum) to suppress root-knot nematodes (meloidogyne arenaria), southern blight (sclerotium rolfsii), and aflatoxigenic fungi (aspergillus sp.) in peanut (arachis hypogaea l.) and to assess shifts in microbial populations following crop rotation. switchgrass did not support populations of root-knot nematodes but supported high populations of nonparasitic nematodes. peanut with no nematicide applied and ...200219265915
protein variability in meloidogyne spp. (nematoda:meloidogynidae) revealed by two-dimensional gel electrophoresis and mass spectrometry.total protein variation as revealed by two-dimensional electrophoresis (2d-e) was studied in 18 isolates from populations of meloidogyne arenaria (six isolates), meloidogyne incognita (10 isolates), and meloidogyne javanica (one isolate) plus an unclassified isolate. gels (80 x 60 x 0.75 mm) were silverstained and digitized in order to compare their protein patterns. optical density and position of protein patterns were measured using statistical cluster analysis and computer-assisted image anal ...200212645913
genetic diversity of root-knot nematodes from brazil and development of scar markers specific for the coffee-damaging species.rapd markers were used to characterize the genetic diversity and relationships of root-knot nematodes (rkn) (meloidogyne spp.) in brazil. a high level of infraspecific polymorphism was detected in meloidogyne arenaria, meloidogyne exigua, and meloidogyne hapla compared with the other species tested. phylogenetic analyses showed that m. hapla and m. exigua are more closely related to one another than they are to the other species, and illustrated the early divergence of these meiotically reproduc ...200212416618
eggs of tylenchulus semipenetrans inhibit growth of phytophthora nicotianae and fusarium solani in vitro.in previous greenhouse and laboratory studies, citrus seedlings infested with the citrus nematode tylenchulus semipenetrans and later inoculated with the fungus phylophthora nicotianae grew larger and contained less fungal protein in root tissues than plants infected by only the fungus, demonstrating antagonism of the nematode to the fungus. in this study, we determined whether eggs of the citrus nematode t. semipenetrans and root-knot nematode meloidogyne arenaria affected mycelial growth of p. ...200219265943
developmental response of a resistance-breaking population of meloidogyne arenaria on vitis spp.pre- and post-infection resistance mechanisms expressed by vitis rootstocks rs-9 and teleki 5c against second-stage juveniles (j2) of resistance-breaking populations of meloidogyne arenaria were observed and correlated with juvenile development and nematode reproduction. cabernet sauvignon grape was used as a susceptible control for comparison. similar numbers of j2 penetrated teleki 5c and cabernet sauvignon roots. root-tip necrosis, a hypersensitive reaction, occurred in both rootstocks but wa ...200219265904
penetration and development of meloidogyne arenaria on two new grape rootstocks.penetration, development, and reproduction of a virulent 'harmony' population of meloidogyne arenaria was studied on two nematode-resistant grape rootstocks 10-17a and 6-19b. 'cabernet sauvignon' was used as a susceptible control for comparison. plants were inoculated with 100 freshly hatched second-stage juveniles (j2) of m. arenaria. greater numbers of j2 penetrated roots of 'cabernet' than 10-17a, and none penetrated roots of 6-19b 4 days after inoculation (dai). at 7 dai, vermiform j2 advanc ...200219265923
relationship between crop losses and initial population densities of meloidogyne arenaria in winter-grown oriental melon in korea.to determine the economic threshold level, oriental melon (cucumis melo l. cv. geumssaragi-euncheon) grafted on shintozoa (cucurbita maxima x cu. moschata) was planted in plots (2 x 3 m) under a plastic film in february with a range of initial population densities (pi) of meloidogyne arenaria. the relationships of early, late, and total yield to pi measured in september and january were adequately described by both linear regression and the seinhorst damage model. initial nematode densities in s ...200219265907
immunolocalization of a putative cuticular collagen protein in several developmental stages of meloidogyne arenaria, globodera pallida and g. rostochiensis.the monoclonal antibody iacr-ccnj.3d has previously been used to isolate a gene (gp-col-8) with strong similarity to cuticular collagen from a mixed stage globodera pallida cdna expression library. the antibody has also been shown to label specifically the amphidial canal of pre-parasitic second stage juveniles (j2) of several plant nematode species without any reactivity on the cuticular surface, indicating that this protein is either not present or is inaccessible on the cuticular surface. thi ...200212018190
meloidogyne incognita and m. arenaria reproduction on dwarf hollies and lantana.meloidogyne incognita and m. arenaria reproduction and host plant tolerance were assessed in field and greenhouse experiments on seven holly cultivars including ilex glabra 'shamrock', i. vomitoria 'schelling's dwarf', i. cornuta 'carissa', red holly hybrid (ilex little red), and i. crenata 'compacta', 'green luster', and 'helleri' as well as japanese boxwood (buxus microphylla) and two lantana cultivars (lantana camara 'miss huff' and 'new gold'). boxwood had the highest m. arenaria and m. inco ...200119265898
crop rotation and nematicides for management of mixed populations of meloidogyne spp. on tobacco.the effects of crop rotation and the nematicides 1,3-dichloropropene (1,3-d), ethoprop, and fenamiphos on the relative frequency of meloidogyne incognita race 3, m. arenaria race 2, and m. javanica and tobacco yields on a sandy loam soil were determined. cropping sequences altered the species composition and population densities of meloidogyne spp. meloidogyne arenaria and m. incognita predominated when cotton, corn, sorghum, or rye-fallow preceded tobacco. meloidogyne javanica and m. arenaria p ...200119265896
analysis of 1,3-dichloropropene for control of meloidogyne spp. in a tobacco pest management system.1,3-dichloropropene (1,3-d) and nonfumigant nematicides were evaluated for control of meloidogyne spp. and soil and foliar insects in a tobacco pest management system. in a field with a high meloidogyne spp. population density (root gall index 4.0 to 4.5 on a 0 to 10 scale in untreated controls), tobacco yields and crop values increased (482 kg/ha and $1,784/ha for 1, 3-d; 326 kg/ha and $1,206/ha for fenamiphos; 252 kg/ha and $933/ha for ethoprop) with nematicide application over an untreated co ...200119265897
population dynamics of meloidogyne incognita, m. arenaria,and other nematodes and crop yields in rotations of cotton, peanut, and wheat under minimum tillage.wheat, cotton, and peanut were arranged in three cropping sequences to determine the effects of fenamiphos (6.7 kg a.i./ha) and cropping sequence on nematode population densities and crop yields under conservation tillage and irrigation for 6 years. the cropping sequences included a wheat winter cover crop each year and summer crops of cotton every year, peanut every year, or cotton rotated every other year with peanut. the population densities of meloidogyne spp. and helicotylenchus dihystera w ...200019270949
extraction and purification of pasteuria spp. endospores.pasteuria penetrans is an endospore-forming bacterial parasite of root-knot nematodes that has potential as a biological control agent. biochemical investigations of p. penetrans are limited because of difficulty in obtaining large quantities of endospores free of plant debris and contaminating microorganisms. our objective was to develop a technique for extraction and purification of p. penetrans endospores from root-knot nematodes. tomato roots infected with meloidogyne arenaria that was paras ...200019270952
molecular cloning and characterisation of a venom allergen ag5-like cdna from meloidogyne incognita.rna fingerprinting was used to identify rnas that were expressed in parasitic second-stage juveniles of meloidogyne incognita, but absent from or reduced in preparasitic second-stage juveniles. a cdna encoding a putative secretory protein was cloned from a m. incognita second-stage juvenile cdna library by probing with a 0.5kb fragment derived from fingerprinting that was more strongly expressed in parasitic second-stage juveniles. the cdna, named mi-msp-1, contained an open reading frame encodi ...200010675748
resistance in selected corn hybrids to meloidogyne arenaria and m. incognita.a total of 33 corn hybrids were evaluated in a series of greenhouse and field trials to determine if they differed in resistance to either meloidogyne incognita race 3 or m. arenaria race 1. reproduction of m. incognita race 3 and m. arenaria race 1 on the hybrids was also compared. reproduction of m. arenaria differed among corn hybrids after 58 to 65 days in greenhouse experiments; however, reproduction was similar among hybrids in the field experiment. no hybrids were consistently resistant t ...200019271019
rate response of 1,3-dichloropropene for nematode control in spring squash in deep sand soils.the soil fumigant 1,3-dichloropropene (1,3-d) formulated with chloropicrin is viewed as a likely alternative for replacing methyl bromide in florida when the latter is phased out in 2005. therefore, it behooves us to learn more about using 1,3-d in deep, sand soils. two trials were conducted on spring squash to determine the most effective rate of 1,3-d for the control of meloidogyne spp. rates tested included 0, 56, 84, 112, and 168 liters/ha of 1,3-d applied broadcast with conventional chisels ...200019271005
reproductive variability of field populations of meloidogyne spp. on grape rootstocks.variability in penetration, development, and reproduction of two resistance-breaking field pathotypes (pt.) of meloidogyne arenaria, m. incognita, and a population of mixed meloidogyne spp. virulent to grape hosts were compared on two resistant vitis rootstocks 'freedom' and 'harmony' in separate tests. 'cabernet sauvignon' was included as a susceptible host to all four nematode populations. secondstage juveniles (j2) of the mixed population failed to penetrate freedom roots. by contrast, 6% of ...200019270976
cloning and characterization of an extremely conserved satellite dna family from the root-knot nematode meloidogyne arenaria.a new satellite dna family, named pmae, has been cloned from the genome of the phytoparasitic nematode, meloidogyne arenaria (nematoda: tylenchida). it is represented as tandemly repeated sequences with a monomeric unit of 172 bp. the monomers are present at approximately 15700 copies per haploid genome, and represent about 5.3% of the total genomic dna. twenty-seven independent monomers have been cloned and sequenced. the deduced consensus sequence is 70.9% a + t rich, with frequent stretches o ...200010791824
virulence and molecular diversity of parthenogenetic root-knot nematodes, meloidogyne spp.root-knot nematodes (rkn) are sedentary endoparasites causing severe damage to a wide variety of crops, including tomato. among them, the parthenogenetic species meloidogyne arenaria, m. incognita and m. javanica are of particular economic importance. the genetic diversity and relationships of 17 populations belonging to these three major species, either avirulent or virulent against the tomato mi resistance gene, were investigated in order to determine whether (a)virulence of the nematodes coul ...200010692014
genetics and mechanism of resistance to meloidogyne arenaria in peanut germplasm.segregation of resistance to meloidogyne arenaria in six bcf peanut breeding populations was examined in greenhouse tests. chi-square analysis indicated that segregation of resistance was consistent with resistance being conditioned by a single gene in three breeding populations (tp259-3, tp262-3, and tp271-2), whereas two resistance genes may be present in the breeding populations tp259-2, tp263-2, and tp268-3. nematode development in clonally propagated lines of resistant individuals of tp262- ...199919270898
bahiagrass, corn, cotton rotations, and pesticides for managing nematodes, diseases, and insects on peanut.florunner peanut was grown after 1 and 2 years of tifton 9 bahiagrass, corn, cotton, and continuous peanut as whole-plots. pesticide treatments aldicarb (3.4 kg a.i./ha), flutolanil (1.7 kg a.i./ha), aldicarb + flutolanil, and untreated (control) were sub-plots. numbers of meloidogyne arenaria second-stage juveniles in the soil and root-gall indices of peanut at harvest were consistently lower in plots treated with aldicarb and aldicarb + flutolanil than in flutolanil-treated and untreated plots ...199919270889
greenhouse studies on the effect of marigolds (tagetes spp.) on four meloidogyne species.the effects of preplanted marigold on tomato root galling and multiplication of meloidogyne incognita, m. javanica, m. arenaria, and m. hapla were studied. marigold cultivars of tagetes patula, t. erecta, t. signata, and a tagetes hybrid all reduced galling and numbers of second-stage juveniles in subsequent tomato compared to the tomato-tomato control. all four meloidogyne spp. reproduced on t. signata 'tangerine gem'. several cultivars of t. patula and t. erecta suppressed galling and reproduc ...199919270876
survey of crop losses in response to phytoparasitic nematodes in the united states for 1994.previous reports of crop losses to plant-parasitic nematodes have relied on published results of survey data based on certain commodities, including tobacco, peanuts, cotton, and soybean. reports on crop-loss assessment by land-grant universities and many commodity groups generally are no longer available, with the exception of the university of georgia, the beltwide cotton conference, and selected groups concerned with soybean. the society of nematologists extension committee contacted extensio ...199919270925
fosthiazate controls meloidogyne arenaria and m. incognita in flue-cured tobacco.the nematicide fosthiazate was evaluated over a 3-year period for management of meloidogyne incognita race 3 (site 1) and m. arenaria race 2 (site 2) in flue-cured tobacco. fosthiazate was applied broadcast and incorporated at rates ranging from 22 to 88 g a.i./100 m(2), and compared with the nematicides fenamiphos (67 g a.i./100 m(2)), 1,3-d (56.1 l/ha, 670 ml/100-m row), and an untreated control. root-gall indices and leaf yields were averaged over the 3-year period. root galling was negativel ...199919270938
host suitability of potential cover crops for root-knot nematodes.several potential cover crops were evaluated for their susceptibility to meloidogyne arenaria race 1, m. incognita race 1, and m. javanica in a series of five greenhouse experiments. no galls or egg masses were observed on roots of castor (ricinus communis), cowpea (vigna unguiculata cv. iron clay), crotalaria (crotalaria spectabilis), or american jointvetch (aeschynomene americana). occasional egg masses (rating </=1.0 on 0-5 scale) were observed on marigold (tagetes minuta) in one test with m. ...199919270926
effect of castor and velvetbean organic amendments on meloidogyne arenaria in greenhouse experiments.effectiveness of castor (ricinus communis) and velvetbean (mucuna deeringiana) amendments was tested for suppression of the root-knot nematode (meloidogyne arenaria) and growth of okra (hibiscus esculentus) in three greenhouse experiments. regression analysis was used to relate nematode population data or plant growth responses to various rates (0, 1, 2, 4, or 8 g/560 cm(3) soil pot) of each amendment in separate experiments. in general, plant growth parameters responded positively to the amendm ...199819274257
resistance to root-knot, reniform, and soybean cyst nematodes in selected soybean breeding lines.soybean breeding lines and reported sources of nematode resistance were evaluated in repeated greenhouse tests for resistance to north carolina populations of the soybean cyst nematode heterodera glycines, reniform nematode rotylenchulus reniformis, and the root-knot nematode species meloidogyne incognita, m. arenaria, and m. arenaria. lines from the soybean breeding program in missouri that had 'hartwig' soybean as a parent were the most resistant to races 1-4 of the soybean cyst nematode and t ...199819274243
management of plant-parasitic nematodes on peanut with selected nematicides in north carolina.field experiments were conducted to determine peanut growth and yield responses to selected fumigant and nonfumigant nemaficide treatments in 1988 and 1989. all treatments with the fumigant 1, 3-d significantly suppressed nematode reproduction (meloidogyne arenaria, m. hapla, and mesocriconema ornatum) and enhanced peanut yields over the other treatments in four tests in 1988. yield increases with the fumigant ranged from about 20% to 100% over the untreated control. test sites in 1989 had lower ...199819274260
high-resolution dna fingerprinting of parthenogenetic root-knot nematodes using aflp analysis.amplified fragment length polymorphism (aflp) analysis has been used to characterize 15 root-knot nematode populations belonging to the three parthenogenetic species meloidogyne arenaria, m. incognita and m. javanica. sixteen primer combinations were used to generate aflp patterns, with a total number of amplified fragments ranging from 872 to 1087, depending on the population tested. two kinds of polymorphic dna fragments could be distinguished: bands amplified in a single genotype, and bands p ...19989465419
effect of meloidogyne arenaria and mulch type on okra in microplot experiments.the effects of perennial peanut (arachis glabrata) hay, an aged yard-waste compost (mainly woodchips), and a control treatment without amendment were determined on two population levels of root-knot (melaidogyne arenaria) nematode over three consecutive years in field microplots. okra (hibiscus esculentus, susceptible to the root-knot nematode) and a rye (secale cereale) cover crop (poor nematode host) were used in the summer and winter seasons, respectively. the organic amendment treatments aff ...199819274256
host status of herbaceous perennials to meloidogyne incognita and m. arenaria.twenty-two different herbaceous perennials were studied for their reaction to separate inoculations of meloidogyne arenaria and m. incognita under greenhouse conditions. perennial taxa that did not develop root-galls following inoculation, and therefore are considered as nonhosts of both nematode species, included species and cultivars of aethionema, fragaria, phlox, and polygonum. echinacea, monarda, and patrinia developed only a few galls. root-galls developed on species and cultivars of achil ...199819274254
resistance of interspecific arachis breeding lines to meloidogyne javanica and an undescribed meloidogyne species.resistance to a peanut-parasitic population of meloidogyne javanica and an undescribed meloidogyne sp. in peanut breeding lines selected for resistance to meloidogyne javanica was examined in greenhouse tests. the interspecific hybrid txag-7 was resistant to reproduction of meloidogyne javanica, m. javanica, and meloidogyne sp. an meloidogyne javanica-resistant selection from the second backcross (bc) of txag-7 to the susceptible cultivar florunner also was resistant to m. javanica but appeared ...199819274226
damage functions for three meloidogyne species on arachis hypogaea in texas.the yield response of florunner peanut to different initial population (pi) densities of meloidogyne arenaria, m. javanica, and an undescribed meloidogyne species (isolate 93-13a) was determined in microplots in 1995 and 1996. seven pi's (0, 0.5, 1, 5, 10, 50, and 100 eggs and j2/500 cm(3) soil) were used for each meloidogyne species in both years. the three species reproduced abundantly on florunner in both years. in 1995, mean reproduction differed among the three species; mean rf values were ...199719274184
response of meloidogyne spp., heterodera glycines, and radopholus similis to tannic acid.tannins, which are water-soluble polyphenols, are toxic to numerous fungi, bacteria, and yeasts. our objectives were to study the efficacy of tannic acid in control of meloidogyne arenaria on tomato and its effects on the behavior of m. arenaria, m. incognita, heterodera glycines, and radopholus similis. three concentrations of tannic acid, 0.1, 1.0, and 10 g/500 cm(3) of soil, were applied preplant (powder) and at-plant (powder and drench) into soil infested with m. arenaria. tannic acid at the ...199719274278
antibodies from chicken eggs as probes for antigens from pasteuria penetrans endospores.the bacteria pasteuria spp. have been identified as among the most promising of several microbial organisms currently under investigation as biological control agents of plant-parasitic nematodes. as part of our goal to develop methods to discriminate isolates of pasteuria penetrans with different host preferences, we investigated the potential of developing antibody probes to identify endospores of different isolates of p. penetrans. polyclonal igy antibodies were raised in chickens against end ...199719274158
differential response to root-knot nematodes in prunus species and correlative genetic implications.responses of 17 prunus rootstocks or accessions (11 from the subgenus amygdalus and 6 from the subgenus prunophora) were evaluated against 11 isolates of meloidogyne spp. including one m. arenaria, four m. incognita, four m. javanica, one m. hispanica, and an unclassified population from florida. characterization of plant response to root-knot nematodes was based on a gall index rating. numbers of females and juveniles plus eggs in the roots were determined for 10 of the rootstocks evaluated aga ...199719274170
temperature effects on the attachment of pasteuria penetrans endospores to meloidogyne arenaria race 1.pasteuria penetrans is a gram positive bacterium that prevents meloidogyne spp. from reproducing and diminishes their ability to penetrate roots. the attachment of the endospores to the cuticle of the nematodes is the first step in the life cycle of the bacterium and is essential for its reproduction. as a preliminary study to a field solarization test, the effects of temperature on the attachment of p. penetrans on meloidogyne arenaria race 1 were investigated. preexposing second-stage juvenile ...199719274192
temperature-dependent development of pasteuria penetrans in meloidogyne arenaria.pasteuria penetrans is a promising biological control agent of plant-parasitic nematodes. this study was conducted to determine effects of temperature on the bacterium's development in meloidogyne arenaria. developmental stages of p. penetrans were viewed with a compound microscope and verified with scanning electron microscopy within each nematode at 100 accumulated degree-day intervals by tracking accumulated degree-days at three temperatures (21, 28, and 35 degrees c). five predominant develo ...199719274154
suppression mechanisms of meloidogyne arenaria race 1 by pasteuria penetrans.the biological control of meloidogyne arenaria on peanut (arachis hypogaea) by pasteuria penetrans was evaluated using a six x six factorial experiment in field microplots over 2 years. the main factors were six inoculum levels of second-stage juveniles (j2) of m. arenaria race 1 (0, 40, 200, 1,000, 5,000, and 25,000 j2/microplot, except that the highest level was 20,000 j2/microplot in 1995) and six infestation levels of p. penetrans as percentages of j2 with endospores attached (0, 20, 40, 60, ...199719274127
effects of temperature on resistance in phaseolus vulgaris genotypes and on development of meloidogyne species.phaseolus vulgaris lines with heat-stable resistance to meloidogyne spp. may be needed to manage root-knot nematodes in tropical regions. resistance expression before and during the process of nematode penetration and development in resistant genotypes were studied at pre- and postinoculation temperatures of 24 degrees c and 24 degrees c, 24 degrees c and 28 degrees c, 28 degrees c and 24 degrees c, and 28 degrees c and 28 degrees c. resistance was effective at all temperature regimes examined, ...199719274137
comparison of sequences from the ribosomal dna intergenic region of meloidogyne mayaguensis and other major tropical root-knot nematodes.the unusual arrangement of the 5s ribosomal gene within the intergenic sequence (igs) of the ribosomal cistron, previously reported for meloidogyne arenaria, was also found in the ribosomal dna of two other economically important species of tropical root-knot nematodes, m, incognita and m. javanica. this arrangement also was found in m. hapla, which is important in temperate regions, and m. mayaguensis, a virulent species of concern in west africa. amplification of the region between the 5s and ...199719274129
responses of meloidogyne arenaria and m. incognita to green manures and supplemental urea in glasshouse culture.the recent loss of many effective nematicides has led to renewed interest in alternative methods of nematode management. greenhouse experiments were conducted to determine the effects of rapeseed and velvetbean green manures, and supplemental urea, on the root-knot nematodes meloidogyne arenaria and m. incognita. green manures were incorporated with m. arenaria-infested soil using rates totaling 200,300, and 400 mg n/kg soil. squash plants grown in this soil were evaluated using a gall index and ...199619277190
inheritance of resistance to the root-knot nematode meloidogyne arenaria in myrobalan plum.the inheritance of resistance of the self-incompatible myrobalan plum prunus cerasifera to the root-knot nematode meloidogyne arenaria was studied using first a diallel cross between five parents of variable host suitability (including two highly resistant clones p.1079 and p.2175, a moderate host p.2032, a good host p.2646 and an excellent host p.16.5), followed by the g2 crosses p.16.5 × (p.2646 × p.1079) and p.2646 × (p.16.5 × p.1079). a total of 355 g1 and 72 g2 clones obtained from hard-woo ...199624166553
quantification of endospore concentrations of pasteuria penetrans in tomato root material.six methods for quantification of the endospore concentrations of pasteuria penetrans from tomato roots are described. mortar disruption and machine disruption methods gave the highest estimations (endospores per gram of root material) of 83.7 and 79.0 million, respectively. these methods were significantly superior to incubation bioassay (47.7 million), enzymatic disruption (32.1 million), and enzymatic disruption + flotation (25.8 million) methods. a centrifugation bioassay method gave the low ...199619277345
damage potential and reproduction of meloidogyne incognita race 3 and m. arenaria race 1 on kenaf.the effects of meloidogyne incognita race 3 and m. arenaria race 1 on growth of kenaf cv. everglades 41 was determined under greenhouse conditions. seedlings of kenaf were inoculated with initial population densities (pi) of 0, 625, 1,250, 2,500, 5,000, and 10,000 eggs/plant and placed on greenhouse benches in a randomized complete block design. plant growth and nematode reproduction were assessed 6 and 12 weeks after inoculation. growth suppression of kenaf in response to increasing pi was obse ...199619277193
suppression of meloidogyne arenaria race 1 by soil application of endospores of pasteuria penetrans.the potential of pasteuria penetrans for suppressing meloidogyne arenaria race 1 on peanut (arachis hypogaea) was tested over a 2-year period in a field microplot experiment. endospores of p. penetrans were mass-produced on m. arenaria race 1 infecting tomato plants. endospores were inoculated in the first year only at rates of 0, 1,000, 3,000, 10,000, and 100,000 endospores/g of soil, respectively, into the top 20 cm of microplots that were previously infested with m. arenaria race 1. one peanu ...199619277131
additive effects of meloidogyne arenaria and sclerotinin rolfsii on peanut.field observations have suggested that infection of peanut by meloidogyne arenaria increases the incidence of southern blight caused by sclerotium rolfsii. three factorial experiments in microplots were conducted to determine if interactions between m. arenaria and s. rolfsii influenced final nematode population densities, incidence of southern blight, or pod yield. treatments included four or five initial population densities of m. arenaria and three inoculum rates of s. rolfsii. final nematode ...199619277351
identification of rapd, scar, and rflp markers tightly linked to nematode resistance genes introgressed from arachis cardenasii into arachis hypogaea.two dominant genes conditioning resistance to the root-knot nematode meloidogyne arenaria were identified in a segregating f2 population derived from the cross of 4x (arachis hypogaea x arachis cardenasii)-ga 6 and pi 261942. mae is proposed as the designation for the dominant gene restricting egg number and mag is proposed as the designation for the dominant gene restricting galling. the high levels of resistance in ga 6 were introgressed from a. cardenasii and, therefore, a search to identify ...19968890516
role of nematodes, nematicides, and crop rotation on the productivity and quality of potato, sweet potato, peanut, and grain sorghum.the objective of this experiment was to determine the effects of fenamiphos 15g and short-cycle potato (po)-sweet potato (sp) grown continuously and in rotation with peanut (pe)-grain sorghum (gs) on yield, crop quality, and mixed nematode population densities of meloidogyne arenaria, m. hapla, m. incognita, and mesocriconema ornatum. greater root-gall indices and damage by m. hapla and m. incognita occurred on potato than other crops. most crop yields were higher and root-gall indices lower fro ...199619277157
penetration and post-infectional development and reproduction of meloidogyne arenaria races 1 and 2 on susceptible and resistant soybean genotypes.penetration, post-infectional development, reproduction, and fecundity of meloidogyne arenaria races 1 and 2 were studied on susceptible (cns), partially resistant (jackson), and highly resistant (pi 200538 and pi 230977) soybean genotypes in the greenhouse. the ability to locate and invade roots was similar between races, but more juveniles penetrated roots of susceptible cns than the resistant genotypes. at 10 days after inoculation, 56% and 99% to 100% of race 1 second-stage juveniles were ve ...199619277152
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