Publications

sensitive pcr detection of meloidogyne arenaria, m. incognita, and m. javanica extracted from soil.	we have developed a simple pcr assay protocol for detection of the root-knot nematode (rkn) species meloidogyne arenaria, m. incognita, and m. javanica extracted from soil. nematodes are extracted from soil using baermann funnels and centrifugal flotation. the nematode-containing fraction is then digested with proteinase k, and a pcr assay is carried out with primers specific for this group of rkn and with universal primers spanning the its of rrna genes. the presence of rkn j2 can be detected a ...	2006	19259460
comparison of methods for assessing resistance to meloidogyne arenaria in peanut.	use of resistant cultivars is a desirable approach to manage the peanut root-knot nematode (meloidogyne arenaria). to incorporate resistance into commercially acceptable cultivars requires reliable, efficient screening methods. to optimize the resistance screening protocol, a series of greenhouse tests were done using seven genotypes with three levels of resistance to m. arenaria. the three resistance levels could be separated based on gall indices as early as two weeks after inoculation (wai) u ...	2007	19259486
virulence of meloidogyne spp. and induced resistance in grape rootstocks.	harmony grape rootstock displays resistance to several meloidogyne spp. but that resistance is not durable in commercial vineyard settings. a 2-year experiment in a microplot setting revealed host specificities of two virulent populations of meloidogyne arenaria and an avirulent population of meloidogyne incognita. in a subsequent split-root experiment, the avirulent nematode population was demonstrated to induce resistance to the virulent nematode population. to quantify the level of resistance ...	2007	19259475
observations on the suppression of root-knot nematode (meloidogyne arenaria) on tomato by incorporation of cyanobacterial powder (oscillatoria chlorina) into potting field soil.	experiments were carried out to investigate the nematicidal potential of a cyanobacterium, oscillatoria chlorina, against the root-knot nematode, meloidogyne arenaria on tomato plants grown in pots filled with 500 cm3 of field soil infested with 12-s stage juveniles (j2)/cm3 soil. incorporation of freeze-dried cyanobacterial powder into potted field soil at the rate of 0.2%, 0.4%, 0.6%, 0.8% and 1.0% (w/w) 5 days prior to tomato planting, reduced root galling, final population of m. arenaria and ...	2007	16458501
root vs pod infection by root-knot nematodes on aflatoxin contamination of peanut.	aflatoxins are potent carcinogens produced by some aspergillus spp. infection of peanut (arachis hypogaea) by root-knot nematodes (meloidogyne arenaria) can lead to an increase in aflatoxin contamination of kernels when the plants are subjected to drought stress during pod maturation. it is not clear whether the increased aflatoxin contamination is primarily due to greater invasion of the galled pods by toxigenic aspergillus spp. or whether root galling is also involved. our objective was to det ...	2007	18399500
molecular and morphological characterization of an unusual meloidogyne arenaria population from traveler's tree, ravenala madagascariensis.	an unusual variant of meloidogyne arenaria was discovered on roots of a traveler's tree (ravenala madagascariensis) intended for display at a public arboretum in pennsylvania. the population aroused curiosity by the lack of visible galling on the roots of the infected plant, and the female vulval region was typically surrounded by egg sacs. most morphometrics of the population fit within the ranges reported for m. arenaria, with a mosaic of features in common with either m. platani or other trop ...	2008	19440257
evaluation of an antibiotic-producing strain of pseudomonas fluorescens for suppression of plant-parasitic nematodes.	the antibiotic 2,4-diacetylphloroglucinol (dapg), produced by some strains of pseudomonas spp., is involved in suppression of several fungal root pathogens as well as plant-parasitic nematodes. the primary objective of this study was to determine whether wood1r, a d-genotype strain of dapg-producing p. fluorescens, suppresses numbers of both sedentary and migratory plant-parasitic nematodes. an experiment was conducted in steam-heated soil and included two seed treatments (with wood1r and a cont ...	2009	22736820
population dynamics of meloidogyne arenaria and pasteuria penetrans in a long-term crop rotation study.	the endospore-forming bacterium pasteuria penetrans is an obligate parasite of root-knot nematodes (meloidogyne spp.). the primary objective of this study was to determine the effect of crop sequence on abundance of p. penetrans. the experiment was conducted from 2000 to 2008 at a field site naturally infested with both the bacterium and its host meloidogyne arenaria and included the following crop sequences: continuous peanut (arachis hypogaea) (p-p-p) and peanut rotated with either 2 years of ...	2009	22736828
using fame analysis to compare, differentiate, and identify multiple nematode species.	we have adapted the sherlock(®) microbial identification system for identification of plant parasitic nematodes based on their fatty acid profiles. fatty acid profiles of 12 separate plant parasitic nematode species have been determined using this system. additionally, separate profiles have been developed for rotylenchulus reniformis and meloidogyne incognita based on their host plant, four species and three races within the meloidogyne genus, and three life stages of heterodera glycines. stati ...	2009	22736811
potential of leguminous cover crops in management of a mixed population of root-knot nematodes (meloidogyne spp.).	root-knot nematode is an important pest in agricultural production worldwide. crop rotation is the only management strategy in some production systems, especially for resource poor farmers in developing countries. a series of experiments was conducted in the laboratory with several leguminous cover crops to investigate their potential for managing a mixture of root-knot nematodes (meloidogyne arenaria, m. incognita, m. javanica). the root-knot nematode mixture failed to multiply on mucuna prurie ...	2010	22736854
host status of endophyte-infected and noninfected tall fescue grass to meloidogyne spp.	tall fescue grass cultivars with or without endophytes were evaluated for their susceptibility to meloidogyne incognita in the greenhouse. tall fescue cultivars evaluated included, i) wild-type jesup (e+, ergot-producing endophyte present), ii) endophyte-free jesup (e-, no endophyte present), iii) jesup (max-q, non-ergot producing endophyte) and iv) georgia 5 (e+). peach was included as the control. peach supported greater (p ≤ 0.05) reproduction of m. incognita than all tall fescue cultivars. d ...	2010	22736851
effects of midas® on nematodes in commercial floriculture production in florida.	cut flower producers currently have limited options for nematode control. four field trials were conducted in 2006 and 2007 to evaluate midas® (iodomethane:chloropicrin 50:50) for control of root-knot nematodes (meloidogyne arenaria) on celosia argentea var. cristata in a commercial floriculture production field in southeastern florida. midas (224 kg/ha) was compared to methyl bromide:chloropicrin (98:2, 224 kg/ha), and an untreated control. treatments were evaluated for effects on meloidogyne a ...	2010	22736832
coprinus comatus: a basidiomycete fungus forms novel spiny structures and infects nematode.	nematophagous basidiomycete fungi kill nematodes by trapping, endoparasitizing and producing toxin. in our studies coprinus comatus (o.f.müll. : fr.) pers. is found to be a nematode-destroying fungus; this fungus immobilizes, kills and uses free-living nematode panagrellus redivivus goodey and root-knot nematode meloidogyne arenaria neal. c. comatus produces an unusual structure designated spiny ball. set on a sporophore-like branch, the spiny ball is a burr-like structure assembled with a large ...	2010	21148944
differential gene expression in roots of nematode-resistant and -susceptible peanut (arachis hypogaea) cultivars in response to early stages of peanut root-knot nematode (meloidogyne arenaria) parasitization.	the peanut root-knot nematode (rkn, meloidogyne arenaria) can cause significant yield losses in cultivated peanut (arachis hypogaea). however, molecular events underlying successful rkn infection and host responses in peanut are sparsely understood. using suppression subtractive hybridization (ssh), cdna libraries, enriched with differentially expressed ests, were constructed from rkn-challenged root tissues in the pre-penetration and early infection stages from near-isogenic nematode-resistant ...	2011	20863592
susceptibility of several common subtropical weeds to meloidogyne arenaria, m. incognita, and m. javanica.	experiments were conducted in the greenhouse to assess root galling and egg production of three root-knot nematode species, meloidogyne arenaria, m. incognita, and m. javanica, on several weeds common to florida agricultural land. weeds evaluated were amaranthus retroflexus (redroot pigweed), cyperus esculentus (yellow nutsedge), eleusine indica (goosegrass), portulaca oleracea (common purslane), and solanum americanum (american black nightshade). additionally, although it is recommended as a co ...	2012	23482324
characterization of a root-knot nematode population of meloidogyne arenaria from tupungato (mendoza, argentina).	root-knot nematodes (meloidogyne spp.) are polyphagous plant parasites of global importance. successful host infection depends on the particular interaction between a specific nematode species and race and a specific plant species and cultivar. accurate diagnosis of nematode species is relevant to effective agricultural management; and benefits further from understanding the variability within a single nematode species. here, we described a population of m. arenaria race 2 from mendoza (argentin ...	2012	23481918
rkn lethal db: a database for the identification of root knot nematode (meloidogyne spp.) candidate lethal genes.	root knot nematode (rkn; meloidogyne spp.) is one of the most devastating parasites that infect the roots of hundreds of plant species. rkn cannot live independently from their hosts and are the biggest contributors to the loss of the world's primary foods. rnai gene silencing studies have demonstrated that there are fewer galls and galls are smaller when rnai constructs targeted to silence certain rkn genes are expressed in plant roots. we conducted a comparative genomics analysis, comparing rk ...	2012	23144556
evaluation of cover crops with potential for use in anaerobic soil disinfestation (asd) for susceptibility to three species of meloidogyne.	several cover crops with potential for use in tropical and subtropical regions were assessed for susceptibility to three common species of root-knot nematode, meloidogyne arenaria, m. incognita, and m. javanica. crops were selected based on potential use as organic amendments in anaerobic soil disinfestation (asd) applications. nematode juvenile (j2) numbers in soil and roots, egg production, and host plant root galling were evaluated on arugula (eruca sativa, cv. nemat), cowpea (vigna unguicula ...	2013	24379486
genetic mapping of resistance to meloidogyne arenaria in arachis stenosperma: a new source of nematode resistance for peanut.	root-knot nematodes (rkn; meloidogyne sp.) are a major threat to crops in tropical and subtropical regions worldwide. the use of resistant crop varieties is the preferred method of control because nematicides are expensive, and hazardous to humans and the environment. peanut (arachis hypogaea) is infected by four species of rkn, the most damaging being m. arenaria, and commercial cultivars rely on a single source of resistance. in this study, we genetically characterize rkn resistance of the wil ...	2015	26656152
root transcriptome analysis of wild peanut reveals candidate genes for nematode resistance.	wild peanut relatives (arachis spp.) are genetically diverse and were adapted to a range of environments during the evolution course, constituting an important source of allele diversity for resistance to biotic and abiotic stresses. the wild diploid a. stenosperma harbors high levels of resistance to a variety of pathogens, including the root-knot nematode (rkn) meloidogyne arenaria, through the onset of the hypersensitive response (hr). in order to identify genes and regulators triggering this ...	2015	26488731
pasteuria penetrans for control of meloidogyne incognita on tomato and cucumber, and m. arenaria on snapdragon.	meloidogyne incognita and meloidogyne arenaria are important parasitic nematodes of vegetable and ornamental crops. microplot and greenhouse experiments were conducted to test commercial formulations of the biocontrol agent pasteuria penetrans for control of m. incognita on tomato and cucumber and m. arenaria on snapdragon. three methods of application for p. penetrans were assessed including seed, transplant, and post-plant treatments. efficacy in controlling galling and reproduction of the two ...	2015	26527842
broad meloidogyne resistance in potato based on rna interference of effector gene 16d10.	root-knot nematodes (meloidogyne spp.) are a significant problem in potato (solanum tuberosum) production. there is no potato cultivar with meloidogyne resistance, even though resistance genes have been identified in wild potato species and were introgressed into breeding lines. the objectives of this study were to generate stable transgenic potato lines in a cv. russet burbank background that carry an rna interference (rnai) transgene capable of silencing the 16d10 meloidogyne effector gene, an ...	2015	25861119
mitochondrial genome plasticity among species of the nematode genus meloidogyne (nematoda: tylenchina).	the mitochondrial (mt) genomes of the plant-parasitic root-knot nematodes meloidogyne arenaria, meloidogyne enterolobii and meloidogyne javanica were sequenced and compared with those of three other root-knot nematode species in order to explore the mt genome plasticity within meloidogyne. the mt genomes of m. arenaria, m. enterolobii and m. javanica are circular, with an estimated size of 18.8, 18.9 and 19.6 kb, respectively. compared to other nematodes these mt genomes are larger, due to the p ...	2015	25655462
suitability of zucchini and cucumber genotypes to populations of meloidogyne arenaria, m. incognita, and m. javanica.	the host suitability of five zucchini and three cucumber genotypes to meloidogyne incognita (mipm26) and m. javanica (mj05) was determined in pot experiments in a greenhouse. the number of egg masses (em) did not differ among the genotypes of zucchini or cucumber, but the eggs/plant and reproduction factor (rf) did slightly. m. incognita mipm26 showed lower em, eggs/plant, and rf than m. javanica mj05. examination of the zucchini galls for nematode postinfection development revealed unsuitable c ...	2015	25861120
evaluation of steam and soil solarization for meloidogyne arenaria control in florida floriculture crops.	steam and soil solarization were investigated for control of the root-knot nematode meloidogyne arenaria in 2 yr of field trials on a commercial flower farm in florida. the objective was to determine if preplant steam treatments in combination with solarization, or solarization alone effectively controlled nematodes compared to methyl bromide (mebr). trials were conducted in a field with naturally occurring populations of m. arenaria. treatments were solarization alone, steam treatment after sol ...	2016	27765992
reproduction of meloidogyne incognita race 3 on flue-cured tobacco homozygous for rk1 and/or rk2 resistance genes.	most commercial tobacco cultivars possess the rk1 resistance gene to races 1 and 3 of meloidogyne incognita and race 1 of meloidogyne arenaria, which has caused a shift in population prevalence in virginia tobacco fields toward other species and races. a number of cultivars now also possess the rk2 gene for root-knot resistance. experiments were conducted in 2013 to 2014 to examine whether possessing both rk1 and rk2 increases resistance to a variant of m. incognita race 3 compared to either gen ...	2016	27418700