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effects of site-specific application of aldicarb on cotton in a meloidogyne incognita-infested field.cotton farmers in missouri commonly apply a single rate of aldicarb throughout the field at planting to protect their crop from meloidogyne incognita, even though these nematodes are spatially aggregated. our purpose was to determine the effect of site-specific application of aldicarb on cotton production in a field infested with these nematodes in 1997 and 1998. cotton yields were collected from sites not treated with aldicarb (control), sites receiving aldicarb at the standard recommended rate ...200219265917
characterization of the (gaaa) microsatellite region in the plant parasitic nematode meloidogyne artiellia.microsatellites have become one of the most powerful genetic markers in biology. we have used dna sequencing to characterize a highly variable microsatellite (gaaa) locus in the root-knot nematode meloidogyne artiellia. the use of microsatellite flanking primers produced four amplification products that are defined as electromorphs, based on conventional length criteria. the sequencing of these four amplification products revealed the presence of new variants in the population due to sequence va ...200212137957
inheritance of resistance to the root-knot nematode meloidogyne javanica in lettuce.resistance to the root-knot nematodes meloidogyne spp. would be a valuable attribute of lettuce lactuca sativa l. cultivars grown in tropical regions. the looseleaf lettuce 'grand rapids' is resistant to both m. incognita and m. javanica. resistance to m. incognita has a high heritability, under the control of a single gene locus, in which the 'grand rapids' allele, responsible for resistance (me), has predominantly additive gene action, and has incomplete penetrance and variable expressivity. w ...200214963814
characterization of meloidogyne javanica surface coat with antibodies and their effect on nematode behaviour.the surface coat of the 2nd-stage juveniles (j2) of plant-parasitic nematodes is considered to be involved in interactions with microorganisms in the soil and rhizosphere, as well as with the host plant. characterization of surface antigens might be important in the development of new nematode control strategies. in this study, polyclonal and monoclonal antibodies raised against meloidogyne javanica, m. incognita and other plant-parasitic nematodes were tested for their binding to the surface co ...200212211610
effects of etomopathiogenic nematodes on meloidogyne javanica on tomatoes and soybeans.two hawaiian isolates of steinernema feltiae mg-14 and heterohabditis indica mg-13, a french isolate of s. feltiae sn, and a texan isolate of s. riobrave tx were tested for their efficacy against the root-knot nematode, meloidogyne javanica, in the laboratory and greenhouse. experiments were conducted to investigate the effects of treatment application time and dose on m. javanica penetration in soybean, and egg production and plant development in tomato. two experiments conducted to assess the ...200219265939
preparation of meloidogyne javanica near-isogenic lines virulent and avirulent against the tomato resistance gene mi and preliminary analyses of the genetic variation between the two lines.meloidogyne javanica, reproducing by mitotic parthenogenesis, is an economically important pathogen of a wide range of crops. a pair of near-isogenic lines virulent and avirulent toward the tomato resistance gene mi were prepared for m. javanica by continuously selecting an avirulent population on the resistant tomato cultivar momotaro over 19 generations. random amplified polymorphic dna (rapd) analysis with 102 primers revealed that rapd patterns were highly conserved between the virulent and ...200212182073
cloning and characterization of an esophageal-gland-specific pectate lyase from the root-knot nematode meloidogyne javanica.root-knot nematodes (meloidogynejavanica) are obligate sedentary endoparasites that must penetrate the host root to initiate their life cycle. many enzymes are secreted by the nematode to facilitate host penetration; required enzymes may include pectate lyases and cellulases. using differential screening, a class iii pectate lyase, mj-pel-1 (m. javanica pectate lyase 1), was cloned from a library enriched for esophageal gland genes. dna gel blotting confirmed that the mj-pel-1 gene was of nemato ...200212059103
resistance and host-response of selected plants to meloidogyne megadora.fourteen plant species, including 30 genotypes, were assessed for host suitability to meloidogyne megadora in a growth room at 20 to 28 degrees c. host suitability was based on the gall index (gi) and the reproduction factor (rf):final population density (pf)/initial population density (pi). the presence of distinct galling was observed on roots of six plant species, and reproduction occurred on five of the 14 species tested. three cultivars of cantaloupe (cvs. branco do ribatejo, concerto, and ...200219265922
high-throughput targeted ssr marker development in peach (prunus persica).simple sequence repeats (ssrs) have proven to be highly polymorphic, easily reproducible, codominant markers. however, developing an ssr map is very time consuming and expensive, and most ssrs are not specifically linked to gene loci of immediate interest. the ideal situation would be to combine a high-throughput, relatively inexpensive mapping technique with rapid identification of ssr loci in mapped regions of interest. for this reason, we coupled the high-throughput technique of aflp mapping ...200211962629
registration of m1-2 beet germplasm resistant to root-knot nematode. 200211756310
registration of two root-knot nematode-resistant peanut germplasm lines. 200211756306
effect of artemisia vulgaris rhizome extracts on hatching, mortality, and plant infectivity of meloidogyne megadora.the activity of an ethanolic rhizome extract of artemisia vulgaris against hatching, mortality, host plant infectivity, and galling of the root-knot nematode meloidogyne megadora was investigated. the extract inhibited egg hatch (50% inhibition by 2.35mg/ml) and caused second-stage juvenile mortality (50% lethality at 12 hours' exposure to 55.67 mg/ml), both in a dose-dependent manner. nematode infectivity on phaseolus vulgaris 'bencanta trepar', a susceptible host, decreased in a dose-responsiv ...200319262777
pathogenic variability among isolates of meloidogyne javanica on capsicum annum.meloidogyne javanica isolates were collected from nine districts of uttar pradesh. these isolates showed pathogenic variability when inoculated on the pepper cultivars california wonder and suryamukhi green. meloidogyne javanica that infected suryamukhi green but not california wonder were designated as pepper race 1 and the populations that infected both the cultivars were designated pepper race 2. race 1 was more frequent than race 2 in almora, pauri garhwal, basti, gorakhpur, and deoria, wher ...200319262775
differential display analysis of gene expression in the cytoplasm of giant cells induced in tomato roots by meloidogyne javanica.summary giant cells induced by root-knot nematodes are highly specialized cells which function as transfer cells and provide nutrients to support the growth and reproduction of the nematode. changes in the overall pattern of gene expression in giant cells occur during the formation and maintenance of the nematode feeding cells. differential display analysis has been carried out to detect changes in gene expression in giant cells induced in tomato roots by meloidogyne javanica, using mrna isolate ...200320569396
aphid-induced defense responses in mi-1-mediated compatible and incompatible tomato interactions.the tomato mi-1 gene confers resistance to three species of root-knot nematode and potato aphid. we studied changes in expression of jasmonic acid (ja)- and salicylic acid (sa)-dependent defense genes in response to potato and green peach aphids. we determined changes in three pr proteins, lipoxygenase and proteinase inhibitors i and ii transcripts, locally and systemically in both compatible and incompatible interactions in tomato. transcripts for pr-1 were detected earlier and accumulated to h ...200312906114
meloidogyne javanica chorismate mutase transcript expression profile using real-time quantitative rt-pcr.a developmental expression profile of the meloidodgyne javanica esophageal gland gene chorismate mutase-1 (mj-cm-1) could suggest when in the lifecycle of the nematode the mj-cm-1 product is functional. this study used real-time quantitative rt-pcr to examine the variation in mj-cm-1 transcript levels over six timepoints in the nematode lifecycle: egg, infective second-stage juveniles (inf-j2), 2-day post-inoculation (pi), 7-day pi, 14-day pi, and adult. the mj-cm-1 mrna levels peaked at 2-day p ...200319265979
meloidogyne javanica chorismate mutase 1 alters plant cell development.root-knot nematodes are obligate plant parasites that alter plant cell growth and development by inducing the formation of giant cells for feeding. nematodes inject secretions from their esophageal glands through their stylet and into plant cells to induce giant cell formation. meloidogyne javanica chorismate mutase 1 (mjcm-1) is one such esophageal gland protein likely to be secreted from the nematode as giant cells form. mjcm-1 has two domains, an n-terminal chorismate mutase (cm) domain and a ...200312575746
sensitivity of meloidogyne javanica and tylenchulus semipenetrans to isothiocyanates in laboratory assays.abstract isothiocyanates are released through enzymatic degradation of glucosinolates produced by plants in the family brassicaceae. glucosinolate profiles differ among plant species and the isothiocyanate derivatives differ in their toxicity to nematodes. control of plant-parasitic nematodes in soil by isothiocyanates released from incorporated brassicaceous plant material has been inconsistent. success might be improved with knowledge of the relative toxicities of various isothiocyanates again ...200318943062
the root-knot nematode resistance gene mi-1.2 of tomato is responsible for resistance against the whitefly bemisia tabaci.the tomato gene mi-1.2 confers resistance against root-knot nematodes and some isolates of potato aphid. resistance to the whitefly bemisia tabaci previously has been observed in mi-bearing commercial tomato cultivars, suggesting that mi, or a closely linked gene, is responsible for the resistance. the response of two biotypes of b. tabaci to tomato carrying the cloned mi was compared with that of the isogenic untransformed tomato line moneymaker. our results indicate that mi-1.2 is responsible ...200312848430
leucine-rich repeat-mediated intramolecular interactions in nematode recognition and cell death signaling by the tomato resistance protein mi.the root-knot nematode resistance gene mi from tomato encodes a nucleotide-binding/leucine-rich repeat (nb/lrr) protein with a novel amino-terminal domain compared to related disease-resistance genes. the closely linked paralog mi-1.1, which does not confer nematode resistance, encodes a protein 91% identical to the functional copy, mi-1.2. the chimeric construct mi-ds3, which encodes the 161 amino-terminal residues from mi-1.1 fused to the remainder of mi-1.2, induces localized necrosis when tr ...200312787241
root-knot nematode parasitism and host response: molecular basis of a sophisticated interaction.summary taxonomy: eukaryota; metazoa; nematoda; chromadorea; order tylenchida; tylenchoidea; heteroderidae; genus meloidogyne. physical properties: microscopic-non-segmented worms. meloidogyne species can reproduce by apomixis, facultative meiotic parthenogenesis or obligate mitotic parthenogenesis. obligate biotrophic parasites inducing the re-differentiation of plant cells into specialized feeding cells. hosts: meloidogyne spp. can infest more than 3000 plant species including vegetables, frui ...200320569382
a new root-knot nematode, meloidogyne baetica n. sp. (nematoda: heteroderidae), parasitizing wild olive in southern spain.abstract high infection rates of wild olive (olea europaea sp. sylvestris) feeder roots and soil infestation by a new root-knot nematode were found in sandy soil at vejer de la frontera (cádiz), southern spain. morphometric traits and analyses of the nematode esterase electrophoretic pattern as well as of the internal transcribed spacer 1 (its1)-5.8s gene and d2-d3 fragment of the 28s gene of rdna showed that specimens differed clearly from known root-knot nematodes. studies of host-parasite rel ...200318944092
does the presence of detached root border cells of zea mays alter the activity of the pathogenic nematode meloidogyne incognita?abstract the root-knot nematode meloidogyne incognita is a major pathogen of a range of important crops. currently, control is typically achieved by the use of nematicides. however, recent work suggests that manipulating the ability of roots to slough off border cells, which then act as a decoy to the nematode, can significantly decrease damage to the roots. we investigated the attractiveness of border cells to m. incognita and the response of the nematode to border cells in close proximity. we ...200318944094
a set of genes differentially expressed between avirulent and virulent meloidogyne incognita near-isogenic lines encode secreted proteins.a cdna-amplification fragment length polymorphism (aflp)-based strategy has been used to identify genes differentially expressed between two pairs of near-isogenic lines (nil) of the root-knot nematode meloidogyne incognita either avirulent or virulent against the tomato mi resistance gene. gene expression profiles from infective second-stage juveniles (j2) were compared, and 22 of the 24,025 transcript-derived fragments (tdf) generated proved to be differential, i.e., present in both avirulent ...200314651341
induction of tolerance to root-knot nematode by oxycom.oxycom applications increased plant growth and population levels of meloidogyne incognita on susceptible tomato. a single oxycom drench at 2,500 ppm applied 7 days prior to inoculation with m. incognita provided remediation of plant growth measured 63 days later. this occurred without reducing nematode population levels. follow-up drenches at 2,500 ppm at 10-day intervals stunted shoots and roots (p = 0.05). the same application rates at 20-day intervals did not reduce plant growth. plants recei ...200319262766
a profile of putative parasitism genes expressed in the esophageal gland cells of the root-knot nematode meloidogyne incognita.identifying parasitism genes encoding proteins secreted from a nematode's esophageal gland cells and injected through its stylet into plant tissue is the key to understanding the molecular basis of nematode parasitism of plants. meloidogyne incognita parasitism genes were cloned by microaspirating the cytoplasm from the esophageal gland cells of different parasitic stages to provide mrna to create a gland cell-specific cdna library by long-distance reverse-transcriptase polymerase chain reaction ...200312744507
management of root-knot nematode in tomato lycopersicon esculentum, mill, with biogas slurry.the effect of biogas slurry application on the severity of root-knot nematode, meloidogyne incognita, attack on tomato cv. co-1, was tested in the green house with two levels of biogas slurry: 5% and 10% (w/w) added to soil. both the number (3 fruits/plant) and fruit yield (35.2 g/plant) of tomato increased significantly with 10% (w/w) biogas slurry. the plants amended with biogas slurry put up more vegetative growth and tended to flower and fruit much earlier than did those of the control. the ...200312699936
relationship of aerial broad band reflectance to meloidogyne incognita density in cotton.aerial images were obtained on 22 july 1999 and 4 august 2000 from five cotton sites infested with meloidogyne incognita. images contained three broad bands representing the green (500-600 nm), red (600-700 nm), and near-infrared (700-900 nm) spectrum. soil samples were collected and assayed for nematodes in the fall at these sites. sampling locations were identified from images, by locating the coordinates of a wide range of light intensity (measured as a digital number) for each single band, a ...200319265974
the use of olive mill wastes for the control of root-knot nematodes.the suppressive effect of olive mill wastes was tested in a field experiment in sandy soil, infested by meloidogyne incognita, in the province of lecce, southern italy. the field was subdivided in 12 m2 plots distributed at random in four blocks. treatment consisted of different dosages (10, 20 and 40 t/ha) of two composts prepared with either exhausted or fresh olive pomace or raw extraction sewage at rate of 40 or 80 m3/ha. there were four untreated control plots and four plots on which the gr ...200315149102
competition between heterodera glycines and meloidogyne incognita or pratylenchus penetrans: independent infection rate measurements.competition on soybean between heterodera glycines (race 3) and meloidogyne incognita or h. glycines and pratylenchus penetrans were investigated in greenhouse experiments. each pair of nematode species was mixed in 3-ml suspensions at ratios of 1,000:0, 750:250, 500:500, 250:750, and 0:1,000 second-stage juveniles or mixed stages for p. penetrans. nematodes from a whole root system were counted and infection rates standardized per 1,000 nematodes (per replication) prior to testing the null hypo ...200319265967
intercropping cover crops with pineapple for the management of rotylenchulus reniformis.effect of cover crops intercropped with pineapple (ananas comosus) on rotylenchulus reniformis population densities and activity of nematode-trapping fungi (ntf) were evaluated in two cycles of cover crop and pineapple. sunn hemp (crotalaria juncea), rapeseed (brassica napus), african marigold (tagetes erecta), or weeds were intercropped with pineapples. beds planted with sunn hemp or rapeseed had lower population densities of r. reniformis than african marigold, weeds, or pineapple plots during ...200319265973
effects of acibenzolar-s-methyl application to rotylenchulus reniformis and meloidogyne javanica.effects of acibenzolar-s-methyl, an inducer of systemic acquired resistance in plants, on rotylenchulus reniformis and meloidogyne javanica in vitro and in vivo were determined. a single foliar application of acibenzolar at 50 mg/liter (5 ml of solution per plant) to 7-day-old cowpea or soybean seedlings decreased r. reniformis and m. javanica egg production by 50% 30 days after inoculation. the mechanism of acibenzolar on plant-parasitic nematodes was then investigated. acibenzolar at 50 to 200 ...200319265983
non-pathogenic fusarium solani represses the biosynthesis of nematicidal compounds in vitro and reduces the biocontrol of meloidogyne javanica by pseudomonas fluorescens in tomato.the aim of the present investigation was to determine the influence of various fusarium solani strains on the production of nematicidal agent(s) in vitro and biocontrol of meloidogyne javanica in tomato by pseudomonas fluorescens strains cha0 and cha0/pme3424.200312859651
improvement of pseudomonas fluorescens cha0 biocontrol activity against root-knot nematode by the addition of ammonium molybdate.to improve the efficacy of pseudomonas fluorescens cha0 and its genetically modified (gm) derivatives by adding ammonium molybdate to control meloidogyne javanica, the root-knot nematode in mungbean.200312641719
development of a new management strategy for the control of root-knot nematodes (meloidogyne spp) in organic vegetable production.the nematophagous fungus, pochonia chlamydosporia (goddard) zare & gams, has been investigated as a potential biological control agent for use in integrated pest management strategies for meloidogyne incognita (kof & white) chitwood in vegetable crops. the release of the fungus as a biological control agent requires a diagnostic method to monitor its spread in the environment and to gain knowledge of its ecology. only molecular methods are sufficiently discriminating to enable the detection of s ...200312587872
characterization of bacillus thuringiensis ser. jordanica (serotype h71), a novel serovariety isolated in jordan.the novel strain of bacillus thuringiensis j112 isolated from a soil sample in jordan was classified and characterized in terms of toxicity against dipteran and nematode larvae, crystal protein pattern, plasmid profile, and cry gene content. a new name, bacillus thuringiensis serovariety jordanica (h serotype 71), is proposed for the reference strain j112. the parasporal crystal proteins were toxic to 3(rd) instar larvae of drosophila melanogaster and to 2(nd) stage juveniles of root knot nemato ...200312783189
expression of an arabidopsis phosphoglycerate mutase homologue is localized to apical meristems, regulated by hormones, and induced by sedentary plant-parasitic nematodes.we previously isolated a partial soybean cdna clone whose transcript abundance is increased upon infection by the sedentary, endoparasitic soybean cyst nematode heterodera glycines. we now isolated the corresponding full-length cdna and determined that the predicted gene product was similar to the group of cofactor-dependent phosphoglycerate mutase/bisphosphoglycerate mutase enzymes (pgm/bpgm; ec 5.4.2.1/5.4.2.4). we designated the corresponding soybean gene gmpgm. pgm and bpgm are key catalysts ...200315010616
lotus japonicus: a new model to study root-parasitic nematodes.sedentary plant-parasitic nematodes engage in complex interactions, and induce specialized feeding structures by redirecting plant developmental pathways, and parallels have been observed with rhizobial nodule development on legumes. a model legume would greatly facilitate a better understanding of the differences between parasitic (nematode) and mutualistic (rhizobia and mycorrhizae) symbioses, and we have developed lotus japonicus as such a model. conditions for efficient parasitism by root-kn ...200314634154
[diseases and its control on dendrobium].the diseases on the dendrobium plants and their occurrence and damage as well as control methods have been investigated and studied. 11 kinds of fungi, 4 kinds of bacteria, 3 kinds of virus and one root-knot nematode were recorded. to control them effectively, cultural methods such as selecting cultivable field, keeping the field sanitation, enhancing cultural operations are mostly recommended. the application of chemical germicides is also stressed.200314650056
induction of the hahsp17.7g4 promoter by root-knot nematodes: involvement of heat-shock elements in promoter activity in giant cells.root-knot nematodes feed from specialized giant cells induced in the plants that they parasitize. we found that the promoter of the hahsp17.7g4 gene, which encodes a small heat-shock protein involved in embryogenesis and stress responses, directed gus expression in tobacco galls induced by the root-knot nematode meloidogyne incognita. in roots containing a gus reporter fusion to the hahsp17.7g4 promoter, 10% of the galls stained for gus expression 1 to 3 days after infection and the fraction sta ...200314651339
investigation of alternatives to methyl bromide for management of meloidogyne javanica on greenhouse grown tomato.the root galling index and the densities of eggs in roots and juveniles in soil of the root-knot nematode meloidogyne javanica (treub) chitwood on tomato, and the effect of these on crop yield were assessed in greenhouse experiments applying various treatments at two different sites in crete, greece. tomato crops were grown for four cycles by rotating nematode-resistant (first and third spring crops) with susceptible (second and fourth autumn crops) cultivars and receiving the following treatmen ...200314667053
interactions between meloidogyne artiellia, the cereal and legume root-knot nematode, and fusarium oxysporum f. sp. ciceris race 5 in chickpea.abstract in the mediterranean basin, fusarium oxysporum f. sp. ciceris and the root-knot nematode meloidogyne artiellia coinfect chickpea. the influence of root infection (after inoculation with 20 nematode eggs and second-stage juveniles per gram of soil) by two m. artiellia populations, from italy and syria, on the reaction of chickpea lines and cultivars with partial resistance to fusarium wilt (ca 252.10.1.om, ca 255.2.5.0, cps 1, and pv 61) and with complete resistance to f. oxysporum f. sp ...200318943615
temporal formation and immunolocalization of an endospore surface epitope during pasteuria penetrans sporogenesis.the synthesis and localization of an endospore surface epitope associated with the development of pasteuria penetrans was determined using a monoclonal antibody (mab) as a probe. nematodes, uninfected or infected with p. penetrans, were harvested at 12, 16, 24, and 38 days after inoculation (dai) and then examined to determine the developmental stage of the bacterium. vegetative growth of p. penetrans was observed only in infected nematodes harvested at 12 and 16 dai, whereas cells at different ...200319262762
effect of compost and manure soil amendments on nematodes and on yields of potato and barley: a 7-year study.a 7-year study located in prince edward island, canada, examined the influence of compost and manure on crop yield and nematode populations. the compost used in this study consisted of cull waste potatoes, sawdust, and beef manure in a 3:3:1 ratio, respectively. no plant-parasitic nematodes were detected in samples collected from windrow compost piles at 5- and 30-cm depths prior to application on field plots. low population densities of bacterial-feeding nematodes were recovered from compost wi ...200319262763
competition of meloidogyne incognita and rotylenchulus reniformis on cotton following separate and concomitant inoculations.it has been hypothesized rotylenchulus reniformis (rr) has a competitive advantage over meloidogyne incognita (mi) in the southeastern cotton production region of the united states. this study examines the reproduction and development of meloidogyne incognita (mi) and rotylenchulus reniformis (rr) in separate and concomitant infections on cotton. under greenhouse conditions, cotton seedlings were inoculated simultaneously with juveniles (j2) of m. incognita and vermiform adults of r. reniformis ...200319262774
meloidogyne haplanaria n. sp. (nematoda: meloidogynidae), a root-knot nematode parasitizing peanut in texas.meloidogyne haplanaria n. sp. is described and illustrated from specimens parasitizing peanut in texas. the perineal pattern of the female is rounded to oval with a dorsal arch that is high and rounded except for striae near the vulva, which are low with rounded shoulders. the striae are distinctly forked in the lateral field, and punctations often occur as a small group near the tail tip and singly within the whole perineal pattern. the female stylet is 13-16 microm long and has broad, distinct ...200319262770
efficacy of paecilomyces lilacinus (strain 251) for the control of root-knot nematodes.the egg pathogenic fungus paecilomyces lilacinus (strain 251), is a unique strain with a wide range of activity against the most important plant parasitic nematodes. due to increased production capacity by solid state fermentation and a new water dispersible granule (wdg) formulation, this biological nematicide may be used in an integrated approach to control plant parasitic nematodes. dose response experiments were conducted with the root-knot nematode meloidogyne incognita on tomatoes using th ...200315149100
managing the mycorrhizosphere--an approach to sustainable agriculture after the phaseout of methyl bromide?plant health promoting rhizobacteria (phpr) with known biocontrol activity toward the root knot nematode, meloidogyne incognito, were tested for their ability to promote root colonization of arbuscular mycorrhizal fungi on tomato under greenhouse conditions. in addition, bacteria isolated from mycorrhizal spores were tested for their direct biological control activity on nematode early root penetration. the nematode antagonistic rhizobacteria rhizobium etli g12 significantly increased mycorrhiza ...200315149101
study on interaction between root-knot nematode meloidogyne javanica and wilt fungus verticillium dahliae on olive seedlings in greenhouse.verticillium dahliae has been reported as a limiting factor in cotton, olive, potato and tomato fields from several countries in the world. root-knot nematodes meloidogyne javanica causes considerable damage to olive groves in olive growing areas. since the presence of these two pathogens in olive trees and seedlings were confirmed in golestan province, this study was proposed to find the mode of their action and interaction with olive seedlings in greenhouse. the non-defoliant strain of the fun ...200315149103
nematicidal activity of some sri lankan plants.nineteen solvent extracts from seven sri lankan plants were examined for nematicidal activity against the root knot nematode meloidogyne incognita. extracts of allophyhu cobbe, lepisanthes tetraphylla (sapindaceae), sarcococca zeylanica (buxaceae) and hedyotis lawsoniae (rubiaceae) have shown significant nematicidal activity.200312822904
meloidogyne javanica on peanut in florida.a mixed population of meloidogyne arenaria race 1 and m. javanica race 3 is reported on peanut from a field in levy county, florida. confirmation of m. javanica on peanut is based on esterase and malate dehydrogenase isozyme patterns resolved on polyacrylamide slab gels following electrophoresis, and perineal patterns. up to 29% of 290 individual females collected from peanut roots in the field in autumn 2002 showed a typical esterase j3 phenotype for m. javanica. this is the third report of m. ...200319262776
reproduction of meloidogyne spp. on resistant peanut genotypes from three breeding programs.three described species of root-knot nematode parasitize peanut (arachis hypogaea): meloidogyne arenaria race 1 (ma), m. hapla (mh), and m. javanica (mj). peanut cultivars with broad resistance to meloidogyne spp. will be useful regardless of the species present in the field. the objective of this study was to determine whether peanut genotypes with resistance to m. arenaria originating from three different breeding programs were also resistant to m. hapla and m. javanica. the experiment used a ...200319262773
mechanism of resistance to meloidogyne arenaria in the peanut cultivar coan.resistance to meloidogyne arenaria in the peanut cultivar coan is inherited as a single, dominant gene. the mechanism of resistance to m. arenaria in coan was evaluated in three experiments. in the first experiment the number of second-stage juveniles (j2) of m. arenaria penetrating roots of the susceptible cultivar florunner was higher than the number of j2 penetrating roots of the resistant peanut cultivar coan (p < 0.05). in a second experiment it was determined that the root size and number ...200319265984
the heat-stable root-knot nematode resistance gene mi-9 from lycopersicon peruvianum is localized on the short arm of chromosome 6.the tomato gene mi-1 confers resistance to three species of root-knot nematodes, meloidogyne spp. however, the resistance mediated by mi-1 is inactive at soil temperatures above 28 degrees c. previously, we identified and mapped a novel heat-stable nematode resistance gene from the wild species lycopersicon peruvianum accession la2157 on to chromosome 6. here we report further characterization of this heat-stable resistance against three mi-1-avirulent biotypes of meloidogyne javanica, meloidogy ...200312589548
horizontally transferred genes in plant-parasitic nematodes: a high-throughput genomic approach.published accounts of horizontally acquired genes in plant-parasitic nematodes have not been the result of a specific search for gene transfer per se, but rather have emerged from characterization of individual genes. we present a method for a high-throughput genome screen for horizontally acquired genes, illustrated using expressed sequence tag (est) data from three species of root-knot nematode, meloidogyne species.200312801413
analysis and functional classification of transcripts from the nematode meloidogyne incognita.plant parasitic nematodes are major pathogens of most crops. molecular characterization of these species as well as the development of new techniques for control can benefit from genomic approaches. as an entrée to characterizing plant parasitic nematode genomes, we analyzed 5,700 expressed sequence tags (ests) from second-stage larvae (l2) of the root-knot nematode meloidogyne incognita.200312702207
the promoter of the arabidopsis thaliana cel1 endo-1,4-beta glucanase gene is differentially expressed in plant feeding cells induced by root-knot and cyst nematodes.summary transgenic tobacco and arabidopsis thaliana carrying the arabidopsis endo-1,4-beta-glucanase (ec 3.2.1.4) cel1 promoter fused to the beta-glucuronidase (gus) reporter gene were infected with the root-knot nematode, meloidogyne incognita, and either the tobacco cyst nematode, globodera tabacum (tobacco), or beet cyst nematode, heterodera schachtii (arabidopsis). cel1-driven gus expression was detected in cell elongation zones of noninfected plants and within feeding sites (giant-cells) in ...200420565607
feeding cell development by cyst and root-knot nematodes involves a similar early, local and transient activation of a specific auxin-inducible promoter element.summary to study the role of the phytohormone auxin in nematode feeding cell induction and early development, the transcriptional regulation of the artificial auxin-responsive promoter element dr5 was monitored in arabidopsis thaliana roots infected with the cyst nematode heterodera schachtii or the root-knot nematode meloidogyne incognita. for both nematode species, a specific and strong activation of dr5::gusa was observed inside the initial feeding cells at 18 h post inoculation, pointing to ...200420565601
phytoecdysteroids: a novel defense against plant-parasitic nematodes.the phytoecdysteroid, 20-hydroxyecdysone (20e), is a major molting hormone of invertebrates, possibly including nematodes. as 20e is inducible in spinach, the defensive role against plant-parasitic nematodes was investigated. the effects of direct application on nematodes was assessed by treating cereal cyst nematode, heterodera avenae, juveniles with concentrations of 20e from 8.2 x 10(-8) to 5.2 x 10(-5) m before applying to triticum aestivum growing in sand. h. avenae, heterodera schachtii (s ...200415609826
preferential expression of a plant cystatin at nematode feeding sites confers resistance to meloidogyne incognita and globodera pallida.the expression patterns of three promoters preferentially active in the roots of arabidopsis thaliana have been investigated in transgenic potato plants in response to plant parasitic nematode infection. promoter regions from the three genes, tub-1, arsk1 and rpl16a were linked to the gus reporter gene and histochemical staining was used to localize expression in potato roots in response to infection with both the potato cyst nematode, globodera pallida and the root-knot nematode, meloidogyne in ...200417166138
optimizing the efficacy of paecilomyces lilacinus (strain 251) for the control of root-knot nematodes.the egg pathogenic fungus paecilomyces lilacinus (strain 251) is a biocontrol fungus with a potential range of activity to control the worldwide most important plant parasitic nematodes. this biological nematicide may be an useful tool in an integrated approach to control mainly sedentary nematodes. greenhouse experiments were conducted with the root-knot nematodes meloidogyne incognita and m. hapla on tomato. p. lilacinus, formulated as wg (bioact wg), was incorporated into soil inoculated with ...200415759437
comparative analysis of two 14-3-3 homologues and their expression pattern in the root-knot nematode meloidogyne incognita.14-3-3 proteins are highly conserved ubiquitous proteins found in all eukaryotic organisms. they are involved in various cellular processes including signal transduction, cell-cycle control, apoptosis, stress response and cytoskeleton organisation. we report here the cloning of two genes encoding 14-3-3 isoforms from the plant parasitic root-knot nematode meloidogyne incognita, together with an analysis of their expression. both genes were shown to be transcribed in unhatched second stage larvae ...200415157770
microsatellite genetic linkage maps of myrobalan plum and an almond-peach hybrid--location of root-knot nematode resistance genes.inheritance and linkage studies were carried out with microsatellite [or simple sequence repeat (ssr)] markers in a f(1) progeny including 101 individuals of a cross between myrobalan plum ( prunus cerasifera ehrh) clone p.2175 and the almond (prunus dulcis mill.)-peach ( prunus persica l. batsch) hybrid clone gn22 ["garfi" (g) almond x "nemared" (n) peach]. this three-way interspecific prunus progeny was produced in order to associate high root-knot nematode (rkn) resistances from myrobalan and ...200415241595
high-resolution mapping and chromosome landing at the root-know nematode resistance locus ma from myrobalan plum using a large-insert bac dna library.the ma gene for root-knot nematode (rkn)resistance from myrobalan plum (prunus cerasifera l.)confers a complete-spectrum and a heat-stable resistance to meloidogvne spp., conversely to mi-i from tomato,which has a more restricted spectrum and a reduced efficiency at high temperature. this gene was identified from a perennial self-incompatible near-wild rootstock species and lies in cosegregation with the scar marker scaflp2 on the prunus linkage group 7 in a 2.3 cm interval between the scar scal ...200415322755
plant nutrient partitioning in coffee infected with meloidogyne konaensis.two experiments were conducted to assess nutrient partitioning in coffee (coffea arabica cv. typica land race guatemala) infected with meloidogyne konaensis. nutrient levels were quantified from soil, roots, and leaves. in the first experiment, 500-cm3 aliquants of a kealakekua andisol were infested with four initial population densities of m. konaensis ranging from 0 to 1,500 freshly hatched second-stage juveniles. coffee plants (~3 months old) were transplanted into the soil and grown for 25 w ...200419262790
morphological, molecular, and differential-host characterization of meloidogyne floridensis n. sp. (nematoda: meloidogynidae), a root-knot nematode parasitizing peach in florida.a root-knot nematode, meloidogyne floridensis n. sp., is described and illustrated from peach originally collected from gainesville, florida. this new species resembles m. incognita, m. christiei, m. graminicola, and m. hispanica, but with lm and sem observations it differs from these species either by the body length, shape of head, tail and tail terminus of second-stage juveniles, body length and shape of spicules in males, and its distinctive female perineal pattern. this pattern has a high t ...200419262784
nematodes in dryland field crops in the semiarid pacific northwest united states.soils and roots of field crops in low-rainfall regions of the pacific northwest were surveyed for populations of plantparasitic and non-plant-parasitic nematodes. lesion nematodes (pratylenchus species) were recovered from 123 of 130 non-irrigated and 18 of 18 irrigated fields. pratylenchus neglectus was more prevalent than p. thornei, but mixed populations were common. population densities in soil were affected by crop frequency and rotation but not by tillage or soil type (p < 0.05). many fiel ...200419262788
morphological and molecular characterization of meloidogyne mayaguensis isolates from florida.the discovery of meloidogyne mayaguensis is confirmed in florida; this is the first report for the continental united states. meloidogyne mayaguensis is a virulent species that can reproduce on host cultivars bred for nematode resistance. the perineal patterns of m. mayaguensis isolates from florida show morphological variability and often are similar to m. incognita. useful morphological characters for the separation of m. mayaguensis from m. incognita from florida are the male stylet length va ...200419262811
molecular characterization and phylogenetic evaluation of the hsp90 gene from selected nematodes.while multiple genes are optimal for corroborating nematode phylogenies, only a few are commonly used. here we examine the phylogenetic potential of the nuclear hsp90 chaperone gene. we used degenerate primers to obtain partial hsp90 sequences from several plant-parasitic and free-living nematodes. hsp90 was single-copy in heterodera glycines and meloidogyne javanica, similar to the situation for caenorhabditis elegans. the full-length h. glycines hsp90 protein sequence showed homology to sequen ...200419262827
relationship between meloidogyne arenaria and aflatoxin contamination in peanut.damaged and developing kernels of peanut (arachis hypogaea) are susceptible to colonization by fungi in the aspergillus flavus group which, under certain conditions, produces aflatoxins prior to harvest. our objective was to determine whether infection of peanut roots and pods by meloidogyne arenaria increases aflatoxin contamination of the kernels when peanut is subjected to drought stress. the experiment was a completely randomized 2-x-2 factorial with 6 replicates/treatment. the treatment fac ...200419262803
effect of winter cover crops on nematode population levels in north florida.two experiments were conducted in north-central florida to examine the effects of various winter cover crops on plant-parasitic nematode populations through time. in the first experiment, six winter cover crops were rotated with summer corn (zea mays), arranged in a randomized complete block design. the cover crops evaluated were wheat (triticum aestivum), rye (secale cereale), oat (avena sativa), lupine (lupinus angustifolius), hairy vetch (vicia villosa), and crimson clover (trifolium incarnat ...200419262833
feeding and maturation by soybean looper (lepidoptera: noctuidae) larvae on soybean affected by weed, fungus, and nematode pests.feeding and maturation by the soybean looper, pseudoplusia includens (walker) (lepidoptera: noctuidae), were investigated in a 2-yr study on 'davis' soybean, glycine max (l.) merr., grown alone and combined with the weed hemp sesbania, sesbania exaltata (raf.) rybd. ex. a. w. hill, the root-knot nematode, meloidogyne incognita (kofoid & white) chitwood, and the charcoal rot fungus, macrophomina phaseolina (tassi) goid. of the three pests, hemp sesbania had the greatest effects on plant growth an ...200414998122
salicylic acid is part of the mi-1-mediated defense response to root-knot nematode in tomato.the mi-1 gene of tomato confers resistance against three species of root-knot nematode in tomato (lycopersicon esculentum). transformation of tomato carrying mi-1 with a construct expressing nahg, which encodes salicylate hydroxylase, a bacterial enzyme that degrades salicylic acid (sa) to catechol, results in partial loss of resistance to root-knot nematodes. exogenous sa was toxic to roots expressing nahg but not to control roots. this toxicity is most likely due to the production of catechol ...200415077667
cytokinins play opposite roles in lateral root formation, and nematode and rhizobial symbioses.we used the cytokinin-responsive arabidopsis response regulator (arr)5 gene promoter fused to a beta-glucuronidase (gus) reporter gene, and cytokinin oxidase (ckx) genes from arabidopsis thaliana (atckx3) and maize (zmckx1) to investigate the roles of cytokinins in lateral root formation and symbiosis in lotus japonicus. arr5 expression was undetectable in the dividing initial cells at early stages of lateral root formation, but later we observed high expression in the base of the lateral root p ...200415078325
the biocontrol fungus pochonia chlamydosporia shows nematode host preference at the infraspecific level.a rapd-pcr assay was developed and used to test for competitive variability in growth of the nematode biological control fungus pochonia chlamydosporia. saprophytic competence in soil with or without tomato plants was examined in three isolates of the fungus: res 280 (j), originally isolated from potato cyst nematode (pcn) cysts; res 200 (i) and res 279 (s), both originally isolated from root knot nematode (rkn) eggs. viable counts taken at 70 d indicated that i was the best saprophyte followed ...200415119353
interactions between root-knot nematode meloidogyne javanica and fusarium wilt disease, fusarium oxysporum f.sp. melonis in different varieties of melon.fusarium oxysporum f.sp. melonis and root-knot nematode (meloidogyne javanica) are destructive pathogens on cucurbits in varamin area of iran. the interaction between two pathogens was studied on local melon cultivars, garmsar and sooski. inoculum of meloidogyne javanica was prepared on susceptible cultivar, rutgers using single egg mass method in greenhouse. inoculum of fusarium oxysporum f.sp. melonis (race 1) was prepared using richard solution. a concentration of 2 x 10(5) micro conidia of f ...200415759439
location of independent root-knot nematode resistance genes in plum and peach.prunus species express different ranges and levels of resistance to the root-knot nematodes (rkn) meloidogyne spp. in myrobalan plum ( prunus cerasifera), the dominant ma gene confers a high-level and wide-spectrum resistance to the predominant rkn, meloidogyne arenaria, meloidogyne incognita, meloidogyne javanica and the isolate meloidogyne sp. florida which overcomes the resistance of the amygdalus sources. in japanese plum ( prunus salicina), a similar wide-spectrum dominant resistance gene, ...200414569426
trichoderma harzianum enhances the production of nematicidal compounds in vitro and improves biocontrol of meloidogyne javanica by pseudomonas fluorescens in tomato.to determine the influence of soil-borne fungus trichoderma harzianum on the biocontrol performance of pseudomonas fluorescens strain cha0 and its 2,4-diacetylphloroglucinol (dapg) overproducing derivative cha0/pme3424 against meloidogyne javanica.200414746551
liquid culture carbon, nitrogen and inorganic phosphate source regulate nematicidal activity by fluorescent pseudomonads in vitro.the aim of the present investigation was to determine the influence of nutrients on the nematicidal activity by pseudomonas aeruginosa strain ie-6s+ and ps. fluorescens strain cha0 in vitro.200414962038
differential impact of some aspergillus species on meloidogyne javanica biocontrol by pseudomonas fluorescens strain cha0.the aim was to determine the influence of some aspergillus species on the production of nematicidal agent(s) in vitro and biocontrol of meloidogyne javanica in tomato by pseudomonas fluorescens strains cha0 and cha0/pme3424.200415189291
production of leucinostatins and nematicidal activity of australian isolates of paecilomyces lilacinus (thom) samson.to evaluate the relationship between leucinostatin production by paecilomyces lilacinus isolates and their biological activities.200415214724
resistance reactions to meloidogyne trifoliophila in trifolium repens and t. semipilosum.the predominant root-knot nematode in new zealand pastures is meloidogyne trifoliophila, and a recurrent selection program in trifolium repens has developed resistance to this species. no data are available, however, on the mechanisms of resistance in t. repens or resistant genotypes of t. semipilosum. the development of m. trifoliophila in roots of t. repens and t. semipilosum was examined weekly after a 2-day inoculation with eggs. more second-stage juveniles (j2) were found in two resistant g ...200419262831
persistence and suppressiveness of pasteuria penetrans to meloidogyne arenaria race.the long-term persistence and suppressiveness of pasteuria penetrans against meloidogyne arenaria race 1 were investigated in a formerly root-knot nematode suppressive site following 9 years of continuous cultivation of three treatments and 4 years of continuous peanut. the three treatments were two m. arenaria race 1 nonhost crops, bahiagrass (paspalum notatum cv. pensacola var. tifton 9), rhizomal peanut (arachis glabrata cv. florigraze), and weed fallow. two root-knot nematode susceptible wee ...200419262836
cyperus tubers protect meloidogyne incognita from 1,3-dichloropropene.meloidogyne incognita-infected and noninfected tubers of yellow nutsedge (cyperus esculentus) and purple nutsedge (cyperus rotundus) were treated with 56 l/ha 1,3-dichloropropene (1,3-d) in microplots and subsequently examined for tuber and nematode viability in the greenhouse using a chile pepper (capsicum annuum) bioassay system. the study was conducted three times. nutsedge tuber viability and m. incognita harbored in both yellow and purple nutsedge tubers were unaffected by 1,3-d treatment. ...200419262797
effects of glucosinolates and their enzymatic hydrolysis products via myrosinase on the root-knot nematode meloidogyne incognita (kofoid et white) chitw.the root-knot nematode meloidogyne incognita (kofoid et white) chitw. is responsible for large yield losses in several horticultural crops. fumigation with chemicals has been efficient in fighting this soil pest, but it clearly shows a negative environmental impact. thus, it is necessary to find an environmentally friendly alternative to control this nematode and meet the requirements imposed by world regulation to ban some chemical fumigants in the world after 2005. the glucosinolate-myrosinase ...200415506804
biochemical changes in glomus fasciculatum colonized roots of lycopersicon esculentum in presence of meloidogyne incognita.glasshouse experiments were conducted to elicit biochemical substantiation for the observed difference in resistance to nematode infection in roots colonized by mycorrhiza, and susceptibility of the fresh flush of roots of the same plant that escaped mycorrhizal colonization. tomato roots were assayed for their biochemical profiles with respect to total proteins, total phenols, indole acetic acid, activities of polyphenol oxidase, phenylalanine ammonia lyase and indole acetic acid oxidase. the r ...200415339037
effect of crotalaria juncea amendment on squash infected with meloidogyne incognita.two greenhouse experiments were conducted to examine the effect of crotalaria juncea amendment on meloidogyne incognita population levels and growth of yellow squash (cucurbita pepo). in the first experiment, four soils with a long history of receiving yard waste compost (ywc+), no-yard-waste compost (ywc-), conventional tillage, or no-tillage treatments were used; in the second experiment, only one recently cultivated soil was used. half of the amount of each soil received air-dried residues of ...200419262819
isolation and characterization of pra1, a trypsin-like protease from the biocontrol agent trichoderma harzianum cect 2413 displaying nematicidal activity.mycoparasitic trichoderma strains secrete a complex set of hydrolytic enzymes under conditions related to antagonism. several proteins with proteolytic activity were detected in culture filtrates from t. harzianum cect 2413 grown in fungal cell walls or chitin and the protein responsible for the main activity (pra1) was purified to homogeneity. the enzyme was monomeric, its estimated molecular mass was 28 kda (sds-page), and its isoelectric point 4.7-4.9. the substrate specificity and inhibition ...200415221228
pepper rootstock graft compatibility and response to meloidogyne javanica and m. incognita.resistance of pepper species (capsicum annuum, c. baccatum, c. chinense, c. chacoense, and c. frutescens), cultivars and accessions to the root-knot nematodes meloidogyne incognita race 2 and m. javanica, and their graft compatibility with commercial pepper varieties as rootstocks were evaluated in growth chamber and greenhouse experiments. most of the plants tested were highly resistant to m. javanica but susceptible to m. incognita. capsicum annuum ar-96023 and c. frutescens accessions as root ...200419262798
reproduction of mi-virulent meloidogyne incognita isolates on lycopersicon spp.selection of detectable numbers of mi-virulent root-knot nematodes has necessitated a greater understanding of nematode responses to new sources of resistance. during the course of this research, we compared the reproduction of four geographically distinct mi-virulent root-knot nematode isolates on three resistant accessions of lycopersicon peruvianum. each accession carried a different resistant gene, mi-3, mi-7, or mi-8. all nematode isolates were verified as meloidogyne incognita using diagno ...200419262789
evaluation of asteraceae plants for control of meloidogyne incognita.of the 56 species and 43 genera of asteraceae tested, 9 were highly resistant or immune to meloidogyne incognita and did not form root galls. twenty-six species and six cultivars had 25% or fewer roots galled and were considered moderately resistant to m. incognita. pre-planting cosmos bipinnatus (f190), gaillardia pulchella, tagetes erecta, tithonia diversifolia, or zinnia elegans (f645) reduced root galling and m. incognita j2 in and around ipomoea reptans. amendment of soils with roots, stems ...200419262785
effect of tea dust residues to control root-knot nematode of tomato.in this research, control of tomato root- knot nematode (meloidogyne incognita) was conducted using tea dust residues at different rates. first, the species and race of nematode were identified by employing diagnostic keys. then, with 5 replications in complete randomized design. tea dust residues were used at 9 treatments (0, 5, 10, 15, 20, 25, 30, 35 and 40 g/kg of soil). statistical analysis on mean treatments rates showed that treatment with 25 g/kg soil economically was effective in growth ...200415759440
use of solid-phase subtractive hybridization for the identification of parasitism gene candidates from the root-knot nematode meloidogyne incognita.summary a solid-phase subtractive strategy was used to clone parasitism gene candidates (pgcs) expressed in the oesophageal gland cells of meloidogyne incognita. nematode intestinal first-strand cdna was synthesized directly on magnetic beads and used to enrich for gland-specific sequences by high stringency hybridization to gland-cell mrna. a gland-specific cdna library was created from the nonhybridizing gland-cell mrna by long-distance reverse transcription polymerase chain reaction. subtract ...200420565611
sensitivity of nematode life-history groups to ions and osmotic tensions of nitrogenous solutions.guild designation of nematodes of similar trophic function and life-history strategy provides a basis for using nematode faunal analyses in an integrative assessment of soil food web condition. omnivorous and predaceous nematodes, categorized at the upper end of a colonizer-persister (c-p) continuum of nematode functional guilds are generally not abundant in cropped soil. these nematodes are more sensitive to heavy metal concentrations than those in other c-p groups, but whether sensitivity to a ...200419262791
genome-wide expression profiling of the host response to root-knot nematode infection in arabidopsis.during a compatible interaction, root-knot nematodes (meloidogyne spp.) induce the redifferentiation of root cells into multinucleate nematode feeding cells (giant cells). hyperplasia and hypertrophy of the surrounding cells leads to the formation of a root gall. we investigated the plant response to root-knot nematodes by carrying out a global analysis of gene expression during gall formation in arabidopsis, using giant cell-enriched root tissues. among 22 089 genes monitored with the complete ...200516236154
pineappledb: an online pineapple bioinformatics resource.a world first pineapple est sequencing program has been undertaken to investigate genes expressed during non-climacteric fruit ripening and the nematode-plant interaction during root infection. very little is known of how non-climacteric fruit ripening is controlled or of the molecular basis of the nematode-plant interaction. pineappledb was developed to provide the research community with access to a curated bioinformatics resource housing the fruit, root and nematode infected gall expressed se ...200516202174
effects of jasmonate-induced defenses on root-knot nematode infection of resistant and susceptible tomato cultivars.jasmonates, such as jasmonic acid (ja), are plant-signaling compounds that trigger induced resistance against certain pathogens and a broad range of arthropod herbivores. one goal of this study was to determine the effects of ja-dependent defenses in tomato on root-knot nematodes. another was to determine if the artificial induction of these defenses could enhance nematode control on plants that carry mi-1.2, a nematode resistance gene that is present in many tomato cultivars. at moderate soil t ...200516132206
morphological and molecular characterization of a new root-knot nematode, meloidogyne thailandica n. sp. (nematoda: meloidogynidae), parasitizing ginger (zingiber sp.).a root-knot nematode meloidogyne thailandica n. sp. was discovered on roots of ginger (zingiber spp.) intercepted from thailand in october 2002 by the u.s. department of agriculture animal and plant health inspection service at the port of san francisco. comparison by light microscopy (lm) and scanning electron microscopy (sem) to five other morphologically related species (m. incognita, m. arenaria, m. microcephala, m. megatyla, and m. enterolobii) revealed that the new species differs from the ...200519262883
laser capture microdissection and real-time pcr for measuring mrna in giant cells induced by meloidogyne javanica.the techniques of laser capture microdissection and quantitative rt-pcr were investigated as methods for measuring mrna in giant cells induced by meloidogyne javanica. laser capture microdissection allowed precise sampling of giant cells at 1 to 3 weeks after inoculation. the expression of three genes (a water channel protein gene rb7, a plasma membrane h(+)-atpase (lha4), and a hexose kinase (hxk1) was measured based on mrna extracted from tissue samples and quantitated using reversetranscripti ...200519262878
effects of application strategies of fumigant and nonfumigant nematicides on cantaloupe grown in deep sand soils in florida.a 2-year study was conducted in which three treatment tactics of oxamyl (at planting application, application every 2 weeks, and rescue applications, as determined by crop symptoms) were compared to fumigant treatments with methyl bromide, 1,3-dichloropropene (1,3-d), and 1,3-d plus chloropicrin for management of meloidogyne spp. in 2002, treatments that included 1,3-d produced higher yields as determined both by number and weight of marketable fruit. all treatment tactics relying solely on oxam ...200519262874
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