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aggressiveness and reproduction of four meloidogyne arenaria populations on soybean.aggressiveness and reproduction differed among four geographical populations of m. arenaria on six soybean cultivars in field microplots. these differences were consistent over 3 years. the populations did not differ in virulence; i.e., population by cultivar interactions were not significant. perineal pattern morphology, the north carolina differential host test, chromosome counts of immature oocytes, and esterase phenotypes confirmed that the four populations were m. arenaria. three population ...199119283118
further studies on the role of polyploidy in the evolution of meloidogyne.two tetraploid isolates of meloidogyne hapla, 86p and e289p, with haploid chromosome numbers of 34 and 28, respectively, were studied cytogenetically and biologically in relation to the diploid populations, 86-va (n = 17) and e289-taiwan (n = 14), from which they had been originally isolated. both isolates were quite stable, converting to diploidy at the low rate of about 2.5%. the tetraploid isolate 86p maintained itself in competition with its diploid counterpart in mixed cultures, although an ...199119283121
a collagenolytic fungus, cunninghamella elegans, for biological control of plant-parasitic nematodes.the root-galling index of tomatoes inoculated with meloidogyne javanica was decreased 70% when collagen was used as a soil amendment (0.1% w/w) and 90% when the amendment was supplemented with the collagenolytic fungus cunninghamella elegans. the root-galling index was reduced 80% when the fungus was homogenized in collagen culture medium and added to soil without collagen supplement. culture filtrates of the fungus c. elegans, grown on collagen as a single source of carbon and nitrogen, immobil ...199119283126
population dynamics of meloidogyne chitwoodi on russet burbank potatoes in relation to degree-day accumulation.population dynamics of meloidogyne chitwoodi were studied for 2 years in a commercial potato field and microplots. annual second-stage juvenile (j2) densities peaked at harvest in mid-fall, declined through the winter, and were lowest in early summer. in the field and in one microplot study, population increase displayed trimodal patterns during the 1984 and 1985 seasons. overwintering nematodes produced egg masses on roots by 600-800 degree-days base 5 c (dd) after planting. second-generation a ...199119283128
interaction of meloidogyne javanica with different races of meloidogyne incognita.the interspecific interactions of meloidogyne javanica with races 1, 2, 3, and 4 of m. incognita on tomato were determined. impacts of the interactions on fecundity and morphometrics of females were also examined. mutually inhibitory interactions occurred between m. javanica and the races of m. incognita, but the negative interactions did not reflect in plant growth. numbers of root galls, egg masses, mature females, total population, fecundity, and reproduction factor declined in concomitant tr ...199119283130
efficacy of ethoprop on meloidogyne hapla and m. chitwoodi and enhanced biodegradation in soil.responses of egg masses, free eggs, and second-stage juveniles (j2) ofmeloidogyne hapla and m. chitwoodi to ethoprop were evaluated. the results indicated that j2 were the most sensitive, followed by free eggs and egg masses. in general, m. chitwoodi was more susceptible to ethoprop than m. hapla. ethoprop at 7.2 mug a.i./g soil protected tomato roots from upward migrating m. chitwoodi for 5 weeks. the zone of protection was extended to 10 and 20 cm below the root zone when 3.6 and 7.2 cm water ...199119283141
characterization of species and races of the genus meloidogyne by dna restriction enzyme analysis.total dna of three species of meloidogyne spp., including four subspecific races of m. incognita, were digested separately with ecor i, cla iii, and hind iii and probed with (3)(2)p-labelled total genomic dna from m. incognita race 1 in southern hybridizations. short exposures of southern blots after hind iii digestion revealed patterns that were useful for separating the species. race differences were seen after longer exposures. the dna fragment patterns obtained were scanned with a laser dens ...199119283147
evidence against amplification of four genes in giant cells induced by meloidogyne incognita.giant-cell dna was isolated from pea (pisum sativum) inoculated with meloidogyne incognita and used in slot blots to test for selective sequence amplification. four sequences representing low (ribulose 1,5-bisphosphate carboxylase and actin), mid-level (histone 3), and highly repetitive (large ribosomal repeat) sequence dna were used as probes. known amounts of root-tip dna and giant-cell dna were blotted onto hybridization membranes and probed. the signal strength on autoradiographs containing ...199119283148
interaction of meloidogyne incognita and water stress in two cotton cultivars.a series of controlled-environment experiments were conducted to elucidate the effects of meloidogyne incognita on host physiology and plant-water relations of two cotton (gossypium hirsutum) cultivars that differed in their susceptibility to nematode infection. inoculation of m. incognita-resistant cultivar auburn 634 did not affect growth, stomatal resistance, or components of plant-water potential relative to uninoculated controls. however, nematode infection of the susceptible cultivar stone ...199119283156
development of meloidogyne arenaria on peanut and soybean under two temperature cycles.florunner peanut and three soybean cultivars, centennial, gasoy 17, and wright, were inoculated with 48-hour age cohorts of meloidogyne arenari race 1 second-stage juveniles and placed in a growth chamber set to simulate early season (low temperature) and midseason (high temperature) conditions. percentages of the initial inoculum penetrating roots 4 and 8 days after inoculation were 2-3 times higher in soybean cultivars than in peanut; 25% on susceptible soybean and 9% on peanut. penetration an ...199119283157
reaction of prunus rootstocks to meloidogyne incognita and m. arenaria in spain.prunus rootstocks were evaluated for their reaction to meloidogyne incognita and m. arenaria. most rootstocks were peach-almond hybrids of spanish origin. in one experiment three selections of garfi x nemared (g x n) and hansen-5 were highly resistant to m. incognita, but four other rootstocks were susceptible showing high galling indices and population increases. in two experiments with m. arenaria, the hybrid selections g x n nos. 1 and 9 were immune, gf-305 and hansen-5 were resistant, but ni ...199119283164
resistance in soybean cultivars from maturity groups v-viii to soybean cyst and root-knot nematodes.one hundred thirty-nine cultivars of soybean (glycine max) in maturity groups v, vi, vii, and viii were evaluated in a greenhouse for resistance to heterodera glycines races 3 and 14 and meloidogyne incognita, m. arenaria, and m. javanica. of the cultivars tested, 37% had resistance to h. glycines race 3 alone, 12% had resistance to both races 3 and 14, and 69% exhibited a moderate or high level of resistance to one or more of the meloidogyne spp. however, 24% were susceptible to each race of h. ...199119283166
response of tomato cultigens to meloidogyne javanica and races of meloidogyne incognita.thirty-six cultigens of tomato were evaluated for resistance against meloidogyne javanica and four races of m. incognita with standards and parameters adopted by the international meloidogyne project. most cultigens were susceptible to the nematodes, including some that were previously reported to be resistant to these nematodes. ten accessions, namely pusa-120, calmart vfn, panjab 6.nr-7, ec173898 (72t6), ec173897 (cal-mart), ec173896 (kewalo), cln363bcf-167-1-0, cln363bcf-190-1-0, cln363bcf-34 ...199119283169
resistance to root-knot nematodes in cucumber and horned cucumber.two experiments were conducted in the greenhouse. in one experiment, cucumber (cucumis sativus) and horned cucumber (c. metuliferus) cultigens were evaluated for resistance to four root-knot nematode species (meloidogyne arenaria, m. hapla, m. incognita, and m. javanica), and, in a second experiment, a standard (12-week) test was compared with a rapid (6-week) test. in the first experiment, horned cucumber cultigens varied in response to the meloidogyne species. 'sumter' cucumber was more suscep ...199119283171
race composition of meloidogyne incognita and m. arenaria populations in vegetable fields in uttar pradesh.a total of 1,256 populations of meloidogyne incognita and 442 populations of m. arenaria were collected from vegetables in eight districts of uttar pradesh, india. host differentials were used to identify the host race of each population. all four host races of m. incognita were present in six of the eight districts. in the other two districts, only host races 1, 2, and 4 were found. although frequencies of occurrence of the races differed among districts, races 1 and 2 comprised 62% of all m. i ...199119283172
meloidogyne arenaria populations on soybean.the distribution of meloidogyne spp. was determined in the piedmont and coastal plains soybean production areas of south carolina. meloidogyne arenaria, m. incognita, and m. javanica were found in six of seven counties surveyed, with some populations consisting of two or more species. because m. arenaria populations did not reproduce on peanut (arachis hypogaea cv. florunner), they were designated as host race 2. severity of root galling, shoot and root growth, seed yield, and nematode reproduct ...199119283177
cotton as a rotation crop for the management of meloidogyne arenaria and sclerotium rolfsii in peanut.the value of cotton (gossypium hirsutum cv. deltapine 90) in rotation with peanut (arachis hypogaea cv. florunner) for the management of root-knot nematode (meloidogyne arenaria) and southern blight (sclerotium rolfsii) was studied for 6 years in a field at the wiregrass substation in southeast alabama. peanut yields following either 1 or 2 years of cotton (c-p and c-c-p, respectively) were higher than those of peanut monoculture without nematicide [p(-)]. at-plant application of aldicarb to con ...199119283179
rotations of bahiagrass and castorbean with peanut for the management of meloidogyne arenaria.the relative value of 'hale' castorbean (ricinus communis) and 'pensacola' bahiagrass (paspalum notatum) as rotational crops for the management of meloidogyne arenaria and southern blight (sclerotium rolfsii) in 'florunner' peanut (arachis hypogaea) production was studied for 3 years in a field experiment in southeast alabama. peanut following 2 years of castorbean (c-c-p) yielded 43% higher than monocultured peanut without nematicide. at-plant application of aldicarb (30.5 g a.i./100 m row in a ...199119283180
rotations of soybean with tropical corn and sorghum for the management of nematodes.the relative efficacy of rotations of soybean with sorghum and tropical corn for nematode management was studied for 2 years in a field infested with root-knot (meloidogyne arenaria) and soybean cyst (heterodera glycines, race 14) nematodes. corn, sorghum, and soybean cv. kirby were planted in 1989, and in 1990 the same areas were planted with seven soybean cultivars with and without at-plant application ofaldicarb. corn and sorghum did not support h. glycines, but significant juvenile populatio ...199119283181
evaluation of two soybean cultivars and aldicarb treatment in soil infested with plant-parasitic nematodes.the soybean cultivars davis and leflore were grown in a northern florida experimental site infested with eight species of plant-parasitic nematodes. meloidogyne incognita appeared to become the predominant pathogen, suppressing davis soybean yield regardless of aldicarb treatment. soybean yields of leflore were 1.4-fold to 3.5-fold greater than yields of davis, but aldicarb did not affect yields of either cultivar. davis soybean yield was negatively correlated with the at-plant density of m. inc ...199119283184
comparison of winter and spring soil fumigation with 1,3-d for the management of meloidogyne arenaria on peanut.field experiments were conducted in which the fumigant 1,3-d was applied at broadcast rates of 56 and 112 liters/ha during late winter and spring to two differing soil types in north florida. no advantage was demonstrated in applying the fumigant at the higher rate for the management of meloidogyne arenaria on peanut, and there was no disadvantage to applying a standard rate of the fumigant during winter as opposed to the standard practice of a 2-week preplant treatment. at one site, where rainf ...199119283185
effects of incorporation method of ethoprop and addition of aldicarb on potato tuber infection by meloidogyne hapla.the efficacy of controlling meloidogyne hapla on potato with water incorporation of ethoprop was compared to physical incorporation before planting. the standard practice of aldicarb application for insect control was also evaluated for m. hapla suppression with and without ethoprop. physical incorporation before planting by rototilling or discing reduced (p </= 0.05) tuber infection. postplant water incorporation of ethoprop was not as effective as physical incorporation of ethoprop or postplan ...199119283186
invasion of tomato roots and reproduction of meloidogyne incognita as affected by raw sewage sludge.the antagonistic effects of raw sewage sludge on infection of tomato by meloidogyne incognita were tested in greenhouse pot experiments. sludge was mixed with the soil or added on its surface before and after inoculation of tomato plants with nematode eggs. juvenile penetration was determined 1 and 10 days after inoculation, and 6 weeks later root systems were assessed for nematode reproduction. fewer juveniles penetrated roots in pots with sludge added to the soil than in unamended control pots ...199119283192
host suitability and response of asparagus cultivars to meloidogyne species and races.the host-parasite relationships of asparagus and meloidogyne spp. were examined under greenhouse and microplot conditions. meloidogyne species and races differed greatly in their ability to reproduce on asparagus seedlings. meloidogyne hapla generally failed to reproduce, and m. javanica, m. arenaria race 1, and m. incognita race 3 reproduced poorly, with a reproduction factor (rf = final population/initial population) usually < 1.0. only m. arenaria race 2 and m. incognita races 1 and 4 reprodu ...199219283211
effects of rapeseed and vetch as green manure crops and fallow on nematodes and soil-borne pathogens.in a rapeseed-squash cropping system, meloidogyne incognita race 1 and m. javanica did not enter, feed, or reproduce in roots of seven rapeseed cultivars. both nematode species reproduced at low levels on roots of the third crop of rapeseed. reproduction of m. incognita and m. javanica was high on squash following rapeseed, hairy vetch, and fallow. the application of fenamiphos suppressed (p = 0.05) root-gall indices on squash following rapeseed, hairy vetch, and fallow; and on dwarf essex and c ...199219283212
a novel technique for infesting field sites with encapsulated eggs of meloidogyne spp.eggs of meloidogyne arenaria race 1 were encapsulated in calcium alginate for use as inoculum to infest peanut field plots. some eggs within the capsules remained viable up to 10 weeks after preparation. a field site was successfully infested at peanut planting and (or) 6 weeks later. dual applications of nematode inoculum (at planting and 6 weeks later) were superior to single applications (at planting or 6 weeks after planting). field-site infestation levels at the end of the first year were r ...199219283221
relative damage functions and reproductive potentials of meloidogyne arenaria and m. hapla on peanut.the reproductive potential and damage functions for meloidogyne hapla and m. arenaria race 1 on virginia-type peanuts (arachis hypogaea cv. florigiant) were determined over 2 years in microplot experiments in north carolina. peanut yield suppression and damage to pods as a result of galling were greatest in response to m. arenaria (p = 0.01). damage functions for the two species were adequately described by the quadratic models: yield (g/plot) = 398 - 17.1 (log[pi + 1]) - 17.0(log[pi + 1])(2); ( ...199219283222
damage functions for meloidogyne arenaria on peanut.microplot experiments were conducted in 1989 and 1990 to determine the relationship between yield of peanut (arachis hypogaea) and inoculum density ofmeloidogyne arenaria race 1. nine inoculum densities were used, ranging from 0-200 eggs/100 cm(3) soil (1989) or from 0-100 eggs/100 cm(3) (1990), and each density was replicated 10 times. in 1989, higher final densities (mean of 1,171 juveniles [j2]/100 cm(3) soil) were obtained in plots inoculated with 0.5 to 50 eggs/100 cm(3) soil than in plots ...199219283223
resistance to meloidogyne spp. in allohexaploid wheat derived from triticum turgidum and aegilops squarrosa.expression of resistance to meloidogyne incognita and m. javanica from aegilops squarrosa was studied in a synthetic allohexaploid produced from triticum turgidum var. durum cv. produra and ae. squarrosa g 3489. the reproductive rate of different races of m. incognita and m. javanica, expressed in eggs per gram of fresh root, was low (p < 0.05) on the synthetic allohexaploid and the resistant parent, ae. squarrosa g 3489, compared with different bread and durum wheat cultivars. reproduction of r ...198919287575
influence of glomus intraradices and soil phosphorus on meloidogyne incognita infecting cucumis melo.the interaction among glomus intraradices, meloidogyne incognita, and cantaloupe was studied at three soil phosphorus (p) levels in a greenhouse. all plants grew poorly in soil not amended with p, regardless of mycorrhizal or nematode status. in soil amended with 50 mug p /g soil, m. incognita suppressed the growth of nonmycorrhizal plants by 84%. in contrast, growth of mycorrhizal plants inoculated with m. incognita was retarded by only 21%. a similar trend occurred in plants grown in soil with ...198919287577
pathogenicity of two populations of meloidogyne hapla chitwood on alfalfa and sainfoin.the pathogenicity of two populations of the northern root-knot nematode, meloidogyne hapla chitwood, population 1 (p1) from alfalfa and population 2 (p2) from sainfoin, was studied on both alfalfa and sainfoin for 25 weeks. alfalfa and sainfoin plants inoculated with p2 had significantly (p </= 0.05) higher mortality than plants inoculated with p1. plant stands over all weeks for the uninoculated control, p1, and p2 were 90.5, 78.5, and 64.0% for alfalfa and 84.5, 51.0, and 41.0% for sainfoin, r ...198919287580
overwintering stages of meloidogyne incognita in vitis vinifera.the overwintering of meloidogyne incognita in and around vitis vinifera cv. french colombard roots was studied in a naturally infested vineyard at the kearney agricultural center, in a growth chamber, in inoculated vines in microplots at the university of california, davis, and in a greenhouse. infected roots were sampled at intervals from onset of vine dormancy until plants accumulated about 800 degree days (dd - base 10 c). embryogenesis within eggs, classified as less than or more than 16 cel ...198919287581
preferred temperature of meloidogyne incognita.in laboratory thermal gradients, newly hatched infective juveniles of the plant-parasitic root-knot nematode meloidogyne incognita migrated toward a preferred temperature that was several degrees above the temperature to which they were acclimated. after shifting egg masses to a new temperature, the preferred temperature was reset in less than a day. possible functions of this type of thermotaxis are discussed, including the use of thermal gradients around plant roots to locate hosts and to main ...198919287582
impact of paecilomyces lilacinus inoculum level and application time on control of meloidogyne incognita on tomato.microplot experiments were conducted to evaluate the effects of inoculum level and time of application of paecilomyces lilacinus on the protection of tomato against meioidogyne incognita. the best protection against m. incognita was attained with 10 and 20 g of fungus-infested wheat kernels per microplot which resulted in a threefold and fourfold increase in tomato yield, respectively, compared with tomato plants treated with this nematode alone. greatest protection against this pathogen was att ...198919287585
survival of paecilomyces lilacinus in selected carriers and related effects on meloidogyne incognita on tomato.laboratory and microplot experiments were conducted to determine the influence of carrier and storage of paecilomyces lilacinus on its survival and related protection of tomato against meloidogyne incognita. spores of p. lilacinus were prepared in five formulations: alginate pellets (pellets), diatomaceous earth granules (granules), wheat grain, soil, and soil plus chitin. fungal viability was high in wheat and granules, intermediate in pellets, and low in soil and chitin-amended soil stored at ...198919287586
a technique for preparing perineal patterns of root-knot nematodes for scanning electron microscopy. 198919287589
biological control of meloidogyne hapla on alfalfa and tomato with the fungus meria coniospora.this study was to determine whether arthrobotrys flagrans, a. oligospora, and meria coniospora would control the root-knot nematode meloidogyne hapla on alfalfa and tomato. alfalfa seeds were coated with a fungus-rye powder in 2% cellulose and were planted in infested soil. three-week-old seedlings from seed treated with m. coniospora had 60% and 58% fewer galls in two experiments than did seedlings from untreated seeds. numbers of j2 in the soil were not reduced. plant growth did not improve. w ...198919287596
root tissue response of two related soybean cultivars to infection by lectin-treated meloidogyne spp.treatment of second-stage juveniles (j2) of meloidogyne incognita race 1 and m. javanica with soybean agglutinin, concanavalin a, wheat germ agglutinin, lotus tetragonolobus agglutinin, or limax flavus agglutinin or the corresponding competitive sugars for each of these lectins did not alter normal root tissue response of soybean cultivars centennial and pickett 71 to infection by m. incognita race 1 or m. javanica. giant cells were frequently induced in centennial and pickett 71 roots 5 and 20 ...198919287600
efficacy of oxamyl coated on alfalfa seed with a polymer sticker in pratylenchus and meloidogyne infested soils.a polymer sticker was used as a coating in which oxamyl was applied to seeds of alfalfa cultivar saranac for the control of pratylenchus penetrans and meloidogyne hapla. the sticker, diluted 1:1 (sticker:water) to 1:5, delayed seedling emergence during the first 4 days after planting. by day 13, however, emergence from all sticker treatments was comparable to the control. shoot growth of seedlings at day 21 was less than that of the control only from seeds coated with a 1:1 dilution; root growth ...198919287603
dna complexity of the root-knot nematode (meloidogyne spp.) genome.cot curves derived from renaturation kinetics of sheared denatured dna indicated that the genome of six populations representing the four most common root-knot nematode species (meloidogyne incognita, m. arenaria, m. javanica, and m. hapla) is composed of 20% repetitive and 80% nonrepetitive sequences of dna. cot curves were almost identical, indicating that all populations had a haploid genome of approximately the same size. calculations from an average cot curve gave an estimate of 0.51 x 108 ...198919287606
susceptibility of nevada synthetic xx germplasm to a california race of meloidogyne hapla. 198919287611
esterase and malate dehydrogenase phenotypes in portuguese populations of meloidogyne species.nonspecific esterases and malate dehydrogenases of 1-5 females from 40 root-knot nematode populations from portugal were analyzed by electrophoresis in 0.4-mm-thick polyacrylamide gels. fourteen major bands of esterase activity were detected, corresponding to 10 distinct phenotypes, meloidogyne javanica and m. hapla had distinct species-specific phenotypes. two phenotypes occurred in m. arenaria. the most variability was found among m. incognita populations. of the remaining two phenotypes, one ...198919287618
screening subterranean clover (trifolium spp.) germplasm for resistance to meloidogyne species.this study was conducted to identify lines of subterranean clover (trifolium spp.) with resistance to meloidogyne arenaria (neal, 1989) chitwood, 1949, race 1; m. incognita (kofoid and white, 1919) chitwood, 1949, race 3; and m. javanica (treub, 1885) chitwood, 1949. a collection of 134 subterranean clover lines was evaluated and all had intermediate to high susceptibility. root galling was negatively correlated with both seed and dry matter yields. soil fumigation significantly reduced the nema ...198919287623
monoclonal antibodies to secretory granules in esophageal glands of meloidogyne species.monoclonal antibodies to secretory granules in the dorsal or subventral esophageal glands were generated by injecting balb/c mice with immunogens from preparasitic second-stage juveniles (j2) of meloidogyne incognita. antibodies specific for secretory granules in the j2 subventral esophageal glands or the dorsal gland were identified by indirect immunofluorescence microscopy. only antibodies that reacted with granules in the j2 dorsal gland reacted with the esophageal gland lobe ofm. incognita a ...198919287625
pathological reaction of crested wheatgrass cultivars to four meloidogyne chitwoodi populations.meloidogyne chitwoodi populations from tulelake, california; ft. hall, idaho; beryl, utah; and prosser, washington, significantly (p < 0.05) reduced dry shoot weights of crested wheatgrass (agropyron cristatum l., gaertn. and a. desertorum, fisch. ex link, schult.) cultivars hycrest, fairway, and nordan in experiments conducted in a greenhouse and growth chamber. shoot growth depression, root galling, and nematode reproduction indices were greatest (p < 0.05) on plants inoculated with 5,000 eggs ...198919287637
further details and sem observations on meloidogyne marylandi (nematoda: meloidogynidae).specimens of meloidogyne marylandi from bermuda grass and a population from zoysia grass were examined and compared morphologically by light and electron microscopy. the populations probably are conspecific and the differences noted in the zoysia population, mainly those of second-stage juveniles (j2) with shorter tails, are considered normal variations rather than representing another form. scanning electron microscope observations provided additional details of the perineal pattern and head of ...198919287638
effects and dynamics of a nematode community on maize.relationships between nematode density and yield and between final and preplant population levels were examined in small maize plots on sandy soils in north-central florida. plant-parasitic nematodes present in the community included belonolaimus longicaudatus, criconemella sphaerocephala, meloidogyne incognita, paratrichodorus minor, pratylenchus brachyurus, and a xiphinema sp. plant growth--including stand count, grain yield, stalk weight, and size of young plants--often was inversely correlat ...198919287639
effect of carbamate, organophosphate, and avermectin nematicides on oxygen consumption by three meloidogyne spp.second-stage juveniles (i2) of meloidogyne arenaria consumed more oxygen (p </= 0.05) than m. incognita j2, which in turn consumed more than m. javanica j2 (4,820, 4,530, and 3,970 mul per hour per g nematode dryweight, respectively). decrease in oxygen consumption depended on the nematicide used. except for aldicarb, there was no differential sensitivity among the three nematode species. meloidogyne javanica had a greater percentage decrease (p </= 0.05) in oxygen uptake when treated with aldic ...198919287640
composition of high molecular weight excretions-secretions from infective larvae of meloidogyne javanica.infective larvae (j2) of meloidogyne javanica were incubated in distilled water for up to 14 days, and their high molecular weight (> 1,000 daltons) excretions-secretions (es) were isolated and partially characterized. the es consisted of a mixture of proteins, glycoproteins, and proteoglycans or polysaccharides as revealed by differential staining on sodium dodecyl sulfate-polyacrylamide gel electrophoresis and compositional analysis. carbohydrate, with approximately equal amounts of neutral mo ...198919287641
effects and dynamics of a nematode community on soybean.the relationships between densities of all members of a plant-parasitic nematode community and yield of 'davis' soybean and between final and preplant population levels were examined in small plots on sandy soils in north-central florida. plant-parasitic nematodes present in the community included belonolaimus longicaudatus, criconemella sphaerocephala, meloidogyne incognita, paratrichodorus minor, pratylenchus brachyurus, and xiphinema sp. plant growth, including stand count, soybean yield (kg/ ...198919287643
drip irrigation as a delivery system for infestation of field plots with nematodes.a drip irrigation delivery system was used to infest field sites with the plant-parasitic root-knot nematodes, meloidogyne incognita. juvenile or egg inocula passed through the system without blockage of emitters or harm to the nematodes. field sites so infested were available for experimentation. delivery of approximately 5 x 10 to 10 juveniles or 10 to 3 x 10 eggs per emitter through the drip system resulted in heavy root galling of tomatoes planted next to the drip emitters. nematodes feeding ...198919287648
yield relationships and population dynamics of meloidogyne spp. on flue-cured tobacco.the complex nature of nematode-induced diseases of tobacco, the relationships between nematode levels and damage, the methods of determining these relationships, and the evolving nematode communities on tobacco in eastern north carolina are described. crop damage associated with these pathogens varies with nematode race and species, crop cultivar, microflora, and environmental conditions. root-gall indices as well as initial and mid-season numbers of meloidogyne spp, in soil are useful for estim ...198919287654
managing root-knot on tobacco in the southeastern united states.root-knot nematodes suppress yields of flue-cured tobacco an estimated 0.1 to 4.8% annually in the southeastern united states, even though nematode management practices have been widely adopted. although meloidogyne incognita races 1 and 3 have predominated, m. arenaria, m. javanica, and m. incognita races 2 and 4 are increasingly important. seventy-five percent of the flue-cured tobacco hectarage in north carolina and virginia is rotated on 2-year or 3-year intervals. over half of the hectarage ...198919287655
resistance in triticum and aegilops spp. to meloidogyne chitwoodi.two lines of aegilops squarrosa (g 3489 and g 1279) and triticum cultivars anza, cocorit, produra, chinese spring, nugaines, and a synthetic hexaploid were screened for resistance to meloidogyne chitwoodi. reproduction of m. chitwoodi, expressed as eggs per gram root, was low (p < 0.01) on g 3489 and the synthetic hexaploid. reproduction on all other cultivars tested was high although differences (p < 0.01) existed among them.198919287662
comparison of fumigant and nonfumigant nematicides for control of meloidogyne chitwoodi on potato.the fumigant 1,3-dichloropropene (1,3-d) effectively controlled meloidogyne chitwoodi on russet burbank potato, solanum tuberosum. there was a maximum of 4% infected and galled tubers from the 1,3-d treatment after 2,028 degree-days with a base temperature of 5 c (dd5). this compared to 66% infected and galled tubers in aldicarb at-plant treated plots. soil temperature, as determined by dd5, and timing of chemical applications affected the nematicidal activity on m. chitwoodi (p < 0.05). aldicar ...198919287664
suitability of small grains as hosts of meloidogyne species.seven cultivars of wheat, five of oat, one of rye, and four of barley were tested as hosts for meloidogyne incognita, m. javanica, or m. arenaria under greenhouse conditions where soil temperature ranged from 21 to 34 c. reproduction rates of all nematode species were high on all cultivars, except m. javanica and m. arenaria on 'brooks' and 'florida 501' oat. meloidogyne incognita and m. javanica produced more eggs on roots of 'rutgers' tomato than on cultivars of wheat, oat, rye, or barley.198919287666
resistance to meloidogyne arenaria in arachis spp. germplasm.field and greenhouse evaluations of 116 wild arachis spp. genotypes demonstrated the presence of resistance to reproduction of the root-knot nematode meloidogyne arenaria race 1. resistance in greenhouse tests was based on test lines having </= 2.5% of the number of eggs per gram of roots as did the susceptible a. hypogaea cv. tamnut 74. in field tests, resistant genotypes were identified on the basis of having lower (p = 0.05) final nematode population densities than did tamnut 74. resistance w ...198919287667
host suitability of soybean cultivars for meloidogyne incognita and m. arenaria.the suitability of five maturity group (mg) iii and five mg iv soybean, glycine max, cultivars as hosts for meloidogyne incognita and m. arenaria was evaluated in a greenhouse. 'forrest', a mg v cultivar, was used as the standard of comparison for m. incognita resistance. with m. incognita, root-gall and egg-mass indices and reproductive factors for 'asgrow 3307', 'ffr 398', and 'pioneer 9442' were comparable with those found on forrest. meloidogyne arenaria reproduction was lower (p </= 0.05) o ...198919287669
crops uncommon to alabama for the management of meloidogyne arenaria in peanut.in a 1987 field study juveniles of meloidogyne arenaria assayed at the time of peanut harvest were almost undetectable in plots planted with american jointvetch (aeschynomene americana), castor bean (ricinus communis), partridge pea (cassia fasiculata), sesame (sesamum indicum), and cotton (gossypium hirsutum), whereas plots with peanut (arachis hypogaea) averaged 120 juveniles/100 cm(3) soil. application of aldicarb in peanut resulted in an average of 27 juveniles/100 cm(3) soil. in 1988 all pl ...198919287678
effects of root-knot nematodes on areca catechu.no root galling or egg mass formation was noted on betel palm (areca catechu) 3 or 9 months after inoculation with meloidogyne arenaria, m. hapla, m. incognita races 1 and 3, or m. javanica. compared with uninoculated controls, a reduction (p </= 0.05) in fresh root weight was noted after 3 months with m. incognita race 1 but not with other species or races, no differences (p </= 0.05) in root weight between controls and inoculated plants were observed at 9 months, nor were any differences found ...198919287679
long-term effect of crop rotation on soybean in a field infested with meloidogyne arenaria and heterodera glycines.previous cropping sequence (corn-soybean vs. soybean-soybean) and aldicarb effects on soybean yield and nematode numbers at harvest for soybean cultivars with various combinations of nematode resistance were determined in 1988 in a sandy loam soil infested with meloidogyne arenaria race 2 and heterodera glycines races 3 and 4 at elberta, alabama. yield and nematode numbers differed among cultivars with 'leflore' having the highest yield. aldicarb treatment resulted in increased soybean yield but ...198919287680
molecular diagnosis of meloidogyne species.genetic variation within nuclear and mitochondrial dna of meloidogyne species and host races has been evaluated for the development of root-knot nematode molecular diagnostics. this review summarizes the distinctive features of several useful dna-based assays for plant-parasitic nematodes, focusing upon the direct application of these procedures for meloidogyne detection, identification, and systematics.199019287685
morphological comparison of three host races of meloidogyne javanica.a morphological and morphometric comparison using light microscopy and scanning electron microscopy was made of six populations of meloidogyne javanica belonging to three host races (infective on pepper, peanut, or noninfective on both). the variability of certain morphological characters was studied within these populations, and the reliability of these taxonomic traits was evaluated for usefulness in species identification. the most useful diagnostic characters of m. javanica were head and sty ...199019287689
vertical distribution of plant-parasitic nematodes in sandy soil under soybean.vertical distribution of five plant-parasitic nematodes was examined in two north florida soybean fields in 1987 and 1988. soil samples were collected from 0-15 cm, 15-30 cm, and 30-45 cm deep at each site. soil at the three depths consisted of approximately 96% sand. more than 50% of belonolaimus longicaudatus population densities occurred in the upper 15-cm soil layer at planting, but the species became more evenly distributed through the other depths as the season progressed. criconemella sph ...199019287693
effects of cowpea and maize root leachates on meloidogyne incognita egg hatch. 199019287701
growth of carrot and tomato from oxamyl-coated seed and control of meloidogyne hapla.oxamyl was coated on carrot (daucus carota l. cv. spartan fancy-80) and tomato (lycopersicon esculentum mill. cv. glamour) seeds with a polymer sticker for the control of meloidogyne hapla. the sticker diluted in water 1:1 delayed carrot seedling emergence. oxamyl at 40 mg/ml in a 1:5 dilution of sticker lowered the rate of carrot seedling emergence until day 13 and plant growth until day 28. oxamyl at 20 or 40 mg/ml in a 1:5 dilution of sticker on carrot seeds planted in m. hapla-infested muck ...199019287706
association of verticillium chlamydosporium and paecilomyces lilacinus with root-knot nematode infested soil.population densities of meloidogyne incognita and the nematophagous fungi, paecilomyces lilacinus and verticillium chlamydosporium, were determined in 20 northern california tomato fields over two growing seasons. paecilomyces lilacinus was isolated from three fields, v. chlamydosporium was isolated from one field, and both fungi were isolated from 12 fields. verticillium chlamydosporium numbers were positively correlated with numbers of m. incognita and p. lilacinus. paecilomyces lilacinus numb ...199019287711
response of resistant soybean plant introductions to meloidogyne incognita in field microplots.the response of two soybean plant introductions, pi 96354 and pi 417444, highly resistant to meloidogyne incognita, to increasing initial soil population densities (pi) (0, 31, 125, and 500 eggs/100 cm(3) soil) of m. incognita was studied in field microplots for 2 years. the plant introductions were compared to the cultivars forrest, moderately resistant, and bossier, susceptible to m. incognita. averaged across years, the yield suppressions of bossier, forrest, pi 417444, and pi 96354 were 97, ...199019287716
optimization of mitochondrial dna-based hybridization assays to diagnostics in soil.nucleic acid hybridization among root-knot nematode mitochondrial dnas can be used to identify several meloidogyne species. research was initiated to optimize mitochondrial dna-based molecular diagnostics for the demanding environments likely to be encountered in field isolates. dna hybridization using reconstituted dna-soil mixtures revealed a loss of assay sensitivity ranging from 34% to 92% with four agronomic soils tested. this problem was alleviated by the addition of exogenously added dna. ...199019287721
meloidogyne morocciensis n. sp. (meloidogyninae), a root-knot nematode from morocco.meloidogyne morocciensis n. sp. is described from specimens parasitic on peach rootstock from morocco. this species exhibits a combination of morphological characters similar to m. arenaria, m. incognita, and m. javanica. the perineal pattern of females is oval to squarish with a moderately high to high dorsal arch, and widely spaced, smooth striae; lateral lines are absent. the stylet, 16.5 mum long, has transversely ovoid, set-off knobs. males have a set-off, annulated head region. the large, ...199019287722
effects of planting date, small grain crop destruction, fallow, and soil temperature on the management of meloidogyne incognita.the effects of planting date, rye (secale cereale cv. wren abruzzi) and wheat (triticura aestivum cv. coker 797), crop destruction, fallow, and soil temperature on managing meloidogyne incognita race 1 were determined in a 2-year study. more m. incognita juveniles (j2) and egg-producing adults were found in roots of rye planted 1 october than in roots of rye planted 1 november and wheat planted 1 november and 1 december. numbers of m. incognita adults with and without egg masses were near or bel ...199019287731
effect of temperature on expression of resistance to meloidogyne spp. in common bean (phaseolus vulgaris).the effect of soil temperature on the expression of resistance in several common bean lines carrying resistance to root-knot nematodes (meloidogyne spp.) was studied under controlled temperatures in temperature tank and growth chamber conditions. resistance to m. javanica and m. incognita race 1 in bean lines a315, a328, a445, g1805, and g2618 was stable at 24-30 c. however, there was a significant increase in reproduction of m. javanica on a315, a328, and a445 when temperature was increased fro ...199019287744
identification of single meloidogyne juveniles by polymerase chain reaction amplification of mitochondrial dna.polymerase chain reaction (pcr) was used to amplify a specific 1.8-kb sequence of mitochondrial dna from single juveniles and eggs from 17 populations of meloidogyne incognita, m. hapla, m. javanica, and m. arenaria. approximately 2 mug amplified product were produced per reaction. restriction digestion of the amplified product with hinfi permitted discrimination of clonal lineages of the four species. meloidogyne javanica, however, could not be separated from m. hapla by the enzymes used in the ...199019287752
host range and ecology of isolates of pasteuria spp. from the southeastern united states.isolates of pasteuria penetrans were evaluated for ecological characteristics that are important in determining their potential as biological control agents. isolate p-20 survived without loss of its ability to attach to its host nematode in dry, moist, and wet soil and in soil wetted and dried repeatedly for 6 weeks. some spores moved 6.4 cm (the maximum distance tested) downward in soil within 3 days with percolating water. the isolates varied greatly in their attachment to different nematode ...199019287753
repeated sampling to determine the precision of estimating nematode population densities.the first phase of this study involved repeated samplings of five fields using composite samples of 10, 20, 40, and 80 soil cores, to determine the precision of nematode assays. the second phase focused on randomly selecting two and four 2-ha subunits (data on meloidogyne spp.) of 24 fields ranging from 6 to 40 ha and computing the precision of estimated means for these numbers ofsubunits versus the general field mean (based on all 2-ha subunits). average numbers of nematodes from most samples c ...199019287757
resistance in lycopersicon peruvianum to isolates of mi gene-compatible meloidogyne populations.root-knot nematode resistance of f progeny of an intraspecific hybrid (lycopersicon peruvianum var. glandulosum acc. no. 126443 x l. peruvianum acc. no. 270435), l. esculentum cv. piersol (possessing resistance gene mi), and l. esculentum cv. st. pierre (susceptible) was compared. resistance to 1) isolates of two meloidogyne incognita populations artificially selected for parasitism on tomato plants possessing the mi gene, 2) the wild type parent populations, 3) four naturally occurring resistan ...199019287762
an assessment of progress toward microbial control of plant-parasitic nematodes. 199019287771
spring or fall fumigation for control of meloidogyne spp. on tobacco.tests were conducted in 1987-88 to compare the efficacy of spring or fall fumigant nematicide applications for control ofmeloidogyne arenaria and m. incognita on tobacco. chloropicrin, 1,3-d, methyl isothiocyanate, and a methyl isothiocyanate-l,3-d mixture were applied as row treatments. fenamiphos, fenamiphos + fensulfothion, or ethoprop were applied in the spring as nonfumigant nematicide standards. fumigant nematicides increased yields and reduced galling (p = 0.01) in all four tests. spring ...199019287773
plant-parasitic nematodes and fungi associated with root rot of peas on prince edward island.eight commercial pea fields on prince edward island were sampled in june and july over a 2-year period (1986-87) to determine soil population densities and the incidence of nematodes and fungi associated with root rot of peas. root lesion nematodes (pratylenchus spp.) were the dominant endoparasitic nematodes recovered from roots and soil. low populations of the northern root-knot nematode (meloidogyne hapla) were also present. tylenchorhynchus spp. and paratylenchus spp. were recovered frequent ...199019287779
evaluation of soybean cultivars for production in meloidogyne arenaria race 2-infested soil.field trials with 56 soybean cultivars and breeding lines from public and private sources were conducted from 1986 through 1988 at a site infested with meloidogyne arenaria race 2. differences (p < 0.05) among yields were found each year and yields were negatively correlated (p < 0.01) with root-knot nematode galling. all entries were galled and the highest-yielding entries, 'kirby' and 'coker 6738', were determined to have average yield reductions of 56% and 62%, respectively, when compared wit ...199019287790
cytogenetic status of meloidogyne (hypsoperine) spartinae in relation to other meloidogyne species.four populations of meloidogyne spartinae from the coast of north and south carolina were identical cytogenetically. fourteen rod-shaped chromosomes were present in oogonia and spermatogonia, whereas seven bivalents were observed in oocytes and spermatocytes. there were no distinguishable sex chromosomes. chromosome behavior was similar to that of other meloidogyne species. a slight deviation in morphology of prometaphase bivalents was attributed to an increase in frequency of chiasmata that may ...198719290099
enzymatic relationships and evolution in the genus meloidogyne (nematoda: tylenchida).thirty populations of meloidogyne of diverse geographic origin representing 10 nominal species and various reproductive, cytological, and physiological forms known to exist in the genus were examined to determine their enzymatic relationships. the 184 bands resolved in the study of 27 enzymes were considered as independent characters. pair-wise comparisons of populations were performed in all possible combinations to estimate the enzymatic distances (ed) and coefficients of similarity (s). a phy ...198719290100
nicotine content of tobacco roots and toxicity to meloidogyne incognita.the motility of meloidogyne incognita second-stage juveniles (j2) and their ability to induce root galls in tomato were progressively decreased upon exposure to nicotine at concentrations of 1-100 mug/ml. ec values ranged from 14.5 to 22.3 mug/ml, but j2 motility and root-gall induction were not eliminated at 100 mug/ml nicotine. nicotine in both resistant nc 89 and susceptible nc 2326 tobacco roots was increased significantly 4 days after exposure to m. incognita. the increase was greater in re ...198719290102
evaluation of cultivars, experimental lines and plant introduction collection of sainfoin for resistance to meloidogyne hapla chitwood.stands of several cultivars and experimental lines of sainfoin (onobrychis viciifolia) were severely reduced (92% average loss) in a field naturally infested with meloidogyne hapla. stands of two alfalfa cultivars included in the test were unaffected. in studies conducted in the greenhouse with plants inoculated at the time of seeding, average mortality was 55% for sainfoin entries and 7% for ladak alfalfa. little mortality occurred when plants were inoculated after establishment. three months a ...198719290103
root-knot nematode management and yield of soybean as affected by winter cover crops, tillage systems, and nematicides.management of meloidogyne incognita on soybean as affected by winter small grain crops or fallow, two tillage systems, and nematicides was studied. numbers of m. incognita did not differ in plots planted to wheat and rye. yields of soybean planted after these crops also did not differ. numbers of m. incognita were greater in fallow than in rye plots, but soybean yield was not affected by the two treatments. soybean yields were greater in subsoil-plant than in moldboard plowed plots. ethylene dib ...198719290104
nematode-degree days, a density-time model for relating epidemiology and crop losses in perennials.the impact of meloidogyne hapla on alfalfa (medicago sativa) yield was described by a multiple point damage model as a function of current plant status, cumulative pest stress, and crop history. nematode-degree days (ndd(female symbol)), calculated on a physiologic time scale as total area under the adult female population density curve, were used to express m. hapla parasitism as cumulative nematode dosage. ndd(female symbol) increased exponentially over physiological time at rates relative to ...198719290114
water absorbent polymer aids in the infestation of field sites with meloidogyne eggs. 198719290118
coastal bermudagrass rotations for control of root-knot nematodes:. 198719290119
vertical migration of meloidogyne chitwoodi and m. hapla under controlled temperature.migratory ability of second-stage juveniles (j2) of two meloidogyne chitwoodi races and a m. hapla population were compared in soil-filled columns at 12, 18, and 24 c. j2 of all populations migrated farthest at 18 c and least at 12 c. nematode survival was significantly reduced (p = 0.05) at 24 c.m. chitwoodi j2 migrated further and in greater numbers than m. hapla j2 at all temperatures. a comparison with and without a host plant demonstrated no preferential migration toward the plant. water pe ...198719290123
meloidogyne californiensis n. sp. (nemata: meloidogyninae), parasitic on bulrush, scirpus robustus pursh.meloidogyne californiensis n. sp. is described and illustrated from bulrush scirpus robustus in california. lm and sem studies revealed that this species differs from other known species in the genus meloidogyne especially by the prominent posterior cuticular protuberances in the female, the distinct shape of the perineal pattern which is marked by one prominent stria in the perineum, indistinct lateral lines, many broken discontinuous striae on both sides of the arch, and the excretory pore bei ...198719290131
galling and yields of soybean cultivars grown in meloidogyne arenana-infested soil.field trials with 39 soybean cultivars and five breeding lines from public and private sources were conducted from 1982 through 1985 at sites infested with meloidogyne arenaria. nematode population densities and root-knot galling were measured for each soybean entry. all were efficient hosts for the nematode, and average juvenile numbers in the soil increased 5-50 x from planting to harvest. differences (p < 0.05) in galling were found among entries in each year. centennial, cobb, coker 368, hut ...198719290135
a semi-selective medium for the isolation of paecilomyces lilacinus from soil. 198719290138
effect of meloidogyne incognita on plant nutrient concentration and its influence on the physiology of beans.phaseolus vulgaris plants, 3, 8, 11, and 13 days old, were inoculated with 0, 2,000, 4,000, or 8,000 second-stage meloidogyne incognita larvae and maintained under controlled conditions. the photosynthetic rate and the shoot and root concentration of k, ca, mn, fe, cu, and zn were determined by destructive assay at 1-27-day intervals and by nondestructive assay of leaves, stems, and roots at 27 or 28 days after inoculation. in the destructive assay, the concentration of the elements in the plant ...198719290151
interaction between meloidogyne incognita and hoplolaimus columbus on davis soybean.greenhouse and laboratory experiments were performed to determine if an interaction exists between meloidogyne incognita and hoplolaimus columbus on davis soybean. greenhouse tests were performed with three population levels of m. incognita and h. columbus (0, 1,500, 6,000/1.5-liter pot) separately and in all combinations. dry root weight (drt) declined nonlinearly and dry shoot weight (dst) declined linearly with respect to increasing initial populations of m. incognita and h. columbus. when th ...198719290154
sterol composition and ecdysteroid content of eggs of the root-knot nematodes meloidogyne incognita and m. arenaria.free and esterified sterols of eggs of the root-knot nematodes meloidogyne incognita races 2 and 3 and m. arenaria race 1 were isolated and identified by gas-liquid chromatography-mass spectrometry. the major sterols of eggs of each race were 24-ethylcholesterol (33.4-38.8% of total sterol), 24-ethylcholestanol (18.3-25.3%), 24-methylcholesterol (8.6-11.7%), 24-methylcholestanol (7.7-12.5%), and cholesterol (4.6-11.6%). consequently, the major metabolic transformation performed by meloidogyne fe ...198719290155
selective migration and root penetration by meloidogyne incognita and hoplolaimus columbus on soybean roots in vitro. 198719290161
control of pratylenchus penetrans and meloidogyne hapla and yield response of alfalfa due to oxamyl seed treatments.alfalfa (medicago sativa l. cv. saranac) seed were soaked for 20 minutes in water, acetone, or methanol containing 10 or 50 mg/ml of oxamyl (vydate l) or coated with a 2% aqueous cellulose solution containing the same amounts of oxamyl. seed were analyzed for oxamyl by hplc immediately after treatment and after 9 and 26 months of storage. oxamyl content of alfalfa seed did not decline after 26 months of storage. the effects of seed treatment on growth of alfalfa and nematode control were examine ...198719290170
production and partial characterization of stylet exudate from adult females of meloidogyne incognita.adult females of meloidogyne incognita were excised from tomato roots and incubated in 0.04 m phosphate buffered saline, ph 7.4 for 18-72 hours to allow accumulation of stylet exudate. twenty-four percent of the females produced exudate during the initial 18-hour incubation period; 70% of those females producing exudate initially produced additional exudate during the subsequent 54-hour incubation period. analysis of exudate by sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed t ...198719290172
embryonic and postembryonic development of meloidogyne californiensis abdel-rahman &maggenti, 1987. 198719290178
dna isolation and gc base composition of four root-knot nematode (meloidogyne spp.) genomes.phenol extraction and cesium trifluoroacetate ultracentrifugation were compared for efficiency in the extraction of dna from eggs and second-stage juveniles of four species of meloidogyne. the second method proved to be more satisfactory in that it yielded larger amounts of dna, shortened the extraction period, and reduced sample handling by eliminating phenol and ether extraction and rnase treatment. it also made possible the extraction of dna: from more than one sample at a time. the mean base ...198819290179
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