Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| effect of zinc supplementation on ecto-adenosine deaminase activity in lambs infected by haemonchus contortus: highlights on acute phase of disease. | haemonchus contortus (order strongylida) is a common parasitic nematode infecting small ruminants and causing significant economic losses worldwide. it induces innate and adaptive immune responses, which are essential for the clearance of this nematode from the host. ecto-adenosine deaminase (e-ada) is an enzyme that plays an important role in the immune system, while zinc (zn) has been found playing a critical role in e-ada catalysis. therefore, the aim of this study was to assess the effect of ... | 2015 | 25639494 |
| immunoprotection in sheep against haemonchus contortus using its thiol-purified excretory/secretory proteins. | excretory/secretory antigen was prepared by culturing live adult worms of haemonchus contortus in rpmi 1640 medium at a concentration of 50 worms per ml in a culture-flask at 37 ˚c for 24 hr and the culture supernatant was used as antigen. the e/s antigen was purified by thiol-sepharose affinity chromatography. on western blot analysis, it was demonstrated that thiol-purified antigen showed a single reactive band at 66 kda. in immunization trial, sheep were administered intramuscularly with 500 ... | 2012 | 25653765 |
| establishment rate of sheep gastrointestinal nematodes in farmed red deer (cervus elaphus). | to investigate the establishment of sheep gastrointestinal nematodes (gin) in red deer, five red deer and five sheep aged 5-6 months were challenged with a mixed burden of sheep gin at a rate of 327l3/kg bodyweight. the lsmean (se) establishment rates (%) for haemonchus contortus, teladorsagia circumcincta, cooperia curticei, trichostrongylus spp. and oesophagostomum+chabertia spp. were 18.6 (0.03), 35.5 (0.04), 30.7 (0.04), 74.9 (0.05), 19.9 (0.06), respectively in sheep and 10.5 (0.03), 1.0 (0 ... | 2015 | 25657087 |
| the emergence of resistance to the benzimidazole anthlemintics in parasitic nematodes of livestock is characterised by multiple independent hard and soft selective sweeps. | anthelmintic resistance is a major problem for the control of parasitic nematodes of livestock and of growing concern for human parasite control. however, there is little understanding of how resistance arises and spreads or of the "genetic signature" of selection for this group of important pathogens. we have investigated these questions in the system for which anthelmintic resistance is most advanced; benzimidazole resistance in the sheep parasites haemonchus contortus and teladorsagia circumc ... | 2015 | 25658086 |
| molecular and biochemical characterisation of abomasal nematode parasites teladorsagia circumcincta and haemonchus contortus phosphofructokinases and their recognition by the immune host. | full length cdnas encoding phosphofructokinase (pfk) were cloned from teladorsagia circumcincta (tcpfk) and haemonchus contortus (hcpfk). tcpfk (2361 bp) and hcpfk (2367 bp) cdna encoded 787 and 789 amino acid proteins respectively. the predicted amino acid sequences showed 98% similarity with each other and 70% with a caenorhabditis elegans pfk. substrate binding sites were completely conserved in both proteins. soluble n-terminal his-tagged pfk proteins were expressed in escherichia coli strai ... | 2015 | 25662436 |
| removal of tapeworm (moniezia spp.) did not increase growth rates of meat-breed lambs in the northern tablelands of nsw. | this experiment tested the hypothesis that growth rates of meat-breed lambs would not be affected by infection with tapeworm (monieza spp.). two experiments, conducted in successive years (2012 and 2013) on a commercial sheep farm on the northern tablelands of nsw, assessed growth rates of meat-breed lambs, between 4 and 6 months of age, following the removal of the cestode, monieza spp. (or commonly referred to as tapeworm). in 2012 and 2013, 93 and 85 lambs respectively were randomly allocated ... | 2015 | 25677979 |
| comparison of internal transcribed spacers and intergenic spacer regions of five common iranian sheep bursate nematodes. | accurate identification of sheep nematodes is a critical point in epidemiological studies and monitoring of drug resistance in flocks. however, due to a close morphological similarity between the eggs and larval stages of many of these nematodes, such identification is not a trivial task. there are a number of studies showing that molecular targets in ribosomal dna (internal transcribed spacer 1, 2 and intergenic spacer) are suitable for accurate identification of sheep bursate nematodes. the ob ... | 2014 | 25678919 |
| status of benzimidazole resistance in haemonchus contortus of goats from different geographic regions of uttar pradesh, india. | present study was designed to survey the status of benzimidazole (bz) resistance in goat flocks of different agro climatic regions of uttar pradesh (u.p.), india by faecal egg count reduction test (fecrt) and allele specific pcr (as-pcr). faecal egg counts and larval culture were made from representative samples of all regions. the results of the faecal culture and pcr-rflp on beta tubulin isotype-1 gene showed haemonchus contortus was predominant species. fecrt results showed bz resistance prev ... | 2015 | 25680901 |
| prevalence of ovine haemonchosis in wukro, ethiopia. | background. haemonchosis caused by haemonchus contortus is a predominant, highly pathogenic, and economically important disease of sheep and goats. objective. assessing the prevalence of haemonchus parasite and its associated risk factors in sheep slaughtered at different restaurants of wukro. methods. cross-sectional study using random sampling from november 2013 to april 2014 in a total of 384 sheep was conducted and spss version 20 software using descriptive statistics was used for data analy ... | 2015 | 25688297 |
| comparative pharmacokinetic and pharmacodynamic response of single and double intraruminal doses of ivermectin and moxidectin in nematode-infected lambs. | to compare the pharmacokinetics, distribution and efficacy (pharmacodynamic response) of intraruminal ivermectin (ivm) and moxidectin (mxd) administered at 0.2 and 0.4 mg/kg to naturally nematode-infected lambs, and to determine the ex vivo accumulation of these anthelmintics by haemonchus contortus. | 2015 | 25689407 |
| st. croix sheep produce a rapid and greater cellular immune response contributing to reduced establishment of haemonchus contortus. | the objective of this study was to determine breed differences in immune response shortly following haemonchus contortus infection. peripheral and local cellular and humoral immune responses were evaluated in 24 st. croix hair lambs and 24 dorset×(finn-rambouillet) wool lambs at 0, 3, 5 and 7 days after infection with 10,000 l3 h. contortus larvae. blood samples taken immediately before harvest revealed no differences in circulating effector cell populations, yet there were significant differenc ... | 2015 | 25698414 |
| haematobiochemical parameters of goats fed tannin rich psidium guajava and carissa spinarum against haemonchus contortus infection in india. | the antihelminthic properties of tannin-rich plants are being explored as an alternative to chemical drugs to minimise the effects of gastro intestinal nematodes (gin). the present study was, therefore, conducted to investigate the effect of condensed tannins (ct), obtained from regional tanniferous tree leaves, in the haemonchus contortus infected goats on the heamatobiochemical parameters to assess the goat health. twelve adult male goats were randomly divided into three equal groups, namely n ... | 2015 | 25698858 |
| variability of resistance in black bengal goats naturally infected with haemonchus contortus. | a total 290 black bengal goats (6 buck, 109 doe and 175 kids born from 11 sires) were studied to evaluate the variability of resistance in black bengal goats naturally infected with haemonchus contortus. the variability of resistance in black bengal goat was studied for both genetic and non-genetic factors like village, sex, age dam, sire, dam resistance group and offspring resistance group. male kids have slightly higher resistance than female kids although it was not significant. resistance of ... | 2015 | 25698865 |
| persistent challenge with trichostrongylus colubriformis and haemonchus contortus larvae does not affect growth of meat-breed lambs suppressively treated with anthelmintics when grazing. | this experiment tested the hypothesis that persistent challenge with anthelmintic susceptible trichostrongylus colubriformis and haemonchus contortus larvae would not affect growth of grazing, meat-breed lambs when suppressively treated with anthelmintics. the experiment was a 2×2 factorial design using 6-7 months old white suffolk x border leicester/merino (meat-breed) lambs which were either infected with 2000 t. colubriformis and 300 h. contortus l3/week (if) or remained uninfected (uif) for ... | 2015 | 25725548 |
| a highly conserved, inhibitable astacin metalloprotease from teladorsagia circumcincta is required for cuticle formation and nematode development. | parasitic nematodes cause chronic, debilitating infections in both livestock and humans worldwide, and many have developed multiple resistance to the currently available anthelmintics. the protective collagenous cuticle of these parasites is required for nematode survival and its synthesis has been studied extensively in the free-living nematode, caenorhabditis elegans. the collagen synthesis pathway is a complex, multi-step process involving numerous key enzymes, including the astacin metallopr ... | 2015 | 25736599 |
| two types of galactosylated fucose motifs are present on n-glycans of haemonchus contortus. | n-glycans from the nematode haemonchus contortus (barber pole worm), a parasite of sheep and cattle, were the first to be described to possess up to three fucose residues associated with the n,n'-diacetylchitobiosyl core, two being on the reducing-terminal proximal glcnac and one on the distal core glcnac residue. the assumption was that truncated glycans from this organism with three hexose residues have the composition man3glcnac2fuc1-3. in this study, we have performed hplc and maldi-tof ms/m ... | 2015 | 25740940 |
| kinetics and anthelmintic efficacy of topical eprinomectin when given orally to goats. | preliminary data suggest that topical eprinomectin in goat shows an individual variation in anthelmintic efficacy when used off-license at a dose rate of 0.5 or 1.0mg/kg bw. as a result, the use of oral administration of topical formulation of eprinomectin tends to develop in dairy goat farms in france. the plasma levels and milk excretion as well as the anthelmintic efficacy of eprinomectin were determined in goats following oral administration of a topical formulation of the drug at dose rates ... | 2015 | 25744609 |
| haemonchotolerance in west african dwarf goats: contribution to sustainable, anthelmintics-free helminth control in traditionally managed nigerian dwarf goats. | west african dwarf (wad) goats are extremely important in the rural village economy of west africa, but still little is known about their biology, ecology and capacity to cope with gastrointestinal nematode (gin) infections. here, we summarise the history of this breed and explain its economic importance in rural west africa. we review recent work showing that nigerian wad goats are highly trypanotolerant and resist infections with haemonchus contortus more effectively than other breeds of domes ... | 2015 | 25744655 |
| low cost whole-organism screening of compounds for anthelmintic activity. | due to major problems with drug resistance in parasitic nematodes of animals, there is a substantial need and excellent opportunities to develop new anthelmintics via genomic-guided and/or repurposing approaches. in the present study, we established a practical and cost-effective whole-organism assay for the in vitro-screening of compounds for activity against parasitic stages of the nematode haemonchus contortus (barber's pole worm). the assay is based on the use of exsheathed l3 (xl3) and l4 s ... | 2015 | 25746136 |
| effects of dietary supplementation with green tea polyphenols on digestion and meat quality in lambs infected with haemonchus contortus. | ujumqin sheep are susceptible to infection by the gastrointestinal nematode haemonchus contortus, which reduces productivity and total meat yield in sheep. thus, the effects of green tea polyphenol (gtp) supplements (0, 2, 4, or 6g of gtp/kg feed) on dietary nutrient digestibility and meat quality in lambs infected with h. contortus were examined; control lambs were not infected. h. contortus infections did not affect digestion but the apparent digestibilities of nutrients were decreased by diet ... | 2015 | 25746574 |
| decline in faecal worm egg counts in lambs suckling ewes treated with lipophilic anthelmintics: implications for hastening development of anthelmintic resistance. | the aim for this experiment was to look for evidence of milk transfer of anthelmintic actives from ewes to their suckling lambs by reference to lambs' faecal worm egg count (wec). the hypothesis was that wec will decline in lambs suckling ewes treated with anthelmintics known to be lipophilic. one group of lactating border leicester×merino ewes were treated (tx) with a combination of short (2.5mg/kg monepantel) and long-acting (1mg/kg moxidectin long-acting injection and a sustained release of 4 ... | 2015 | 25754351 |
| haemonchus contortus resistance to monepantel in sheep. | in a sheep farm in the netherlands with a suspected haemonchus contortus resistance to monepantel (zolvix®, novartis animal health), a fecal egg count reduction test was carried out in two groups of lambs, according to the method of the world association for the advancement of veterinary parasitology. group 1 was the untreated control group, and group 2 was treated with monepantel at the manufacturer's recommended dose rate. efficacy of treatment with monepantel was 0%. larval identification of ... | 2015 | 25770852 |
| development of novel valerolactam-benzimidazole hybrids anthelmintic derivatives: diffusion and biotransformation studies in helminth parasites. | in the search for new anthelmintics able to overcome the resistance problem against all available drugs in livestock, the synthesis of novel valerolactam-benzimidazole hybrid compounds was reported. this allowed us to obtain these in vitro and in vivo bioactive compounds using nippostrongylus brasiliensis rat model by integrating physiology-based assays and ex vivo diffusion studies. in order to further study those novel hybrid molecules, haemonchus contortus (a sheep gastrointestinal nematode o ... | 2015 | 25816976 |
| mapping and modelling helminth infections in ruminants in europe: experience from gloworm. | mapping and modelling helminth infections in cattle and sheep in europe through advanced geospatial research was one of the main task of gloworm, a three year project (2012-2014) funded under the european commission's (ec) seventh framework programme (fp7). liver flukes as fasciola hepatica and gastrointestinal nematodes, such as haemonchus contortus were chosen for the project since these parasites constitute a major cause of lost productivity in small and large ruminants. the output of the glo ... | 2015 | 25826306 |
| haemonchus contortus: spatial risk distribution for infection in sheep in europe. | haemonchus contortus is a species of gastrointestinal strongyles of primary concern for sheep. this highly pathogenic, blood-feeding helminth negatively influences animal health, welfare and productivity. in order to elucidate the current scenario in terms of prevalence and intensity of h. contortus infection in sheep farms across europe, a standardized crosssectional survey was conducted in three pilot areas in ireland, switzerland and italy, all part of the eu funded gloworm project. two conse ... | 2015 | 25826314 |
| the basic reproduction quotient (q0) as a potential spatial predictor of the seasonality of ovine haemonchosis. | haemonchus contortus is a gastrointestinal nematode parasite of small ruminants, which feeds on blood and causes significant disease and production loss in sheep and goats, especially in warmer parts of the world. the life cycle includes free-living immature stages, which are subject to climatic influences on development, survival and availability, and this species therefore exhibits spatio-temporal heterogeneity in its infection pressure based on the prevailing climate. models that better expla ... | 2015 | 25826315 |
| integrated assessment of ivermectin pharmacokinetics, efficacy against resistant haemonchus contortus and p-glycoprotein expression in lambs treated at three different dosage levels. | the main goals of the current work were: (a) to assess the ivermectin (ivm) systemic exposure and plasma disposition kinetics after its administration at the recommended dose, x5 and x10 doses to lambs, (b) to compare the clinical efficacy of the same ivm dosages in lambs infected with an ivm-resistant isolate of haemonchus contortus, and (c) to assess the expression of the transporter protein p-glycoprotein (p-gp) in h. contortus recovered at 14 days after administration of the ivm dose regimen ... | 2015 | 25841863 |
| macrocyclic lactones differ in interaction with recombinant p-glycoprotein 9 of the parasitic nematode cylicocylus elongatus and ketoconazole in a yeast growth assay. | macrocyclic lactones (mls) are widely used parasiticides against nematodes and arthropods, but resistance is frequently observed in parasitic nematodes of horses and livestock. reports claiming resistance or decreased susceptibility in human nematodes are increasing. since no target site directed ml resistance mechanisms have been identified, non-specific mechanisms were frequently implicated in ml resistance, including p-glycoproteins (pgps, designated abcb1 in vertebrates). nematode genomes en ... | 2015 | 25849454 |
| a molecular characterization of the agonist binding site of a nematode cys-loop gaba receptor. | cys-loop gaba receptors represent important targets for human chemotherapeutics and insecticides and are potential targets for novel anthelmintics (nematicides). however, compared with insect and mammalian receptors, little is known regarding the pharmacological characteristics of nematode cys-loop gaba receptors. here we have investigated the agonist binding site of the cys-loop gaba receptor unc-49 (hco-unc-49) from the parasitic nematode haemonchus contortus. | 2015 | 25850584 |
| identification of novel loci associated with gastrointestinal parasite resistance in a red maasai x dorper backcross population. | gastrointestinal (gi) parasitic infection is the main health constraint for small ruminant production, causing loss of weight and/or death. red maasai sheep have adapted to a tropical environment where extreme parasite exposure is a constant, especially with highly pathogenic haemonchus contortus. this breed has been reported to be resistant to gastrointestinal parasite infection, hence it is considered an invaluable resource to study associations between host genetics and resistance. the aim of ... | 2015 | 25867089 |
| in-vitro anthelmintic activity of three bangladeshi plants against paramphistomum cervi and haemonchus contortus. | conventional plant-based therapies act as an important therapeutic tool for the treatment of worm infections all over the world and continuous evaluation of medicinal plants to find new potential lead compounds should be carried out. | 2015 | 25868001 |
| relative resistance of menz and washera sheep breeds to artificial infection with haemonchus contortus in the highlands of ethiopia. | the aim of this study was to evaluate the relative resistance of menz and washera sheep breeds to artificial infection with haemonchus contortus. the challenge trial was conducted at the debre berhan agricultural research center in ethiopia. a total of 39 (menz = 21, washera = 18) lambs of about 6 months old were used for the trial. all lambs were initially treated against internal parasite using albendazole and fasinex to free them from internal parasites and kept indoors. h. contortus third-st ... | 2015 | 25868601 |
| transmembrane protein 63a is a partner protein of haemonchus contortus galectin in the regulation of goat peripheral blood mononuclear cells. | hco-gal-m and -f were two isoforms of galectin cloned from male and female haemonchus contortus, respectively, and it was demonstrated that recombinant hco-gal-m and -f could act as immune suppressors. however, little is known about the receptors or binding partners of these galectins in the host. the research of the molecular mechanisms that govern the interactions between these galectins and host molecules will fill a gap in our understanding how parasite galectins interact with host cells. | 2015 | 25879191 |
| seasonal patterns of gastrointestinal nematode infection in goats on two lithuanian farms. | this study investigated seasonal changes in naturally acquired gastrointestinal nematode (gin) infections on two lithuanian goat farms with different parasite control practices. | 2015 | 25881323 |
| vaccination of sheep and cattle against haemonchosis. | vaccines against gastrointestinal nematodes are one potential option for the control of parasitic gastroenteritis in ruminants. excretory/secretory (e/s) and hidden antigens are being studied as candidates for vaccines against haemonchus spp., which is a major parasite in cattle and small ruminants that are raised in warm climates. protection has been observed after vaccination with some e/s proteases, particularly cysteine proteases and with some glycans that are abundant on the surfaces and in ... | 2015 | 25891536 |
| profiling of differentially expressed genes in sheep t lymphocytes response to an artificial primary haemonchus contortus infection. | haemonchus contortus is a common bloodsucking nematode causing widespread economic loss in agriculture. upon h. contortus infection, a series of host responses is elicited, especially those related to t lymphocyte immunity. existing studies mainly focus on the general immune responses of sheep t lymphocyte to h. contortus, lacking investigations at the molecular level. the objective of this study was to obtain a systematic transcriptional profiling of the t lymphocytes in h. contortus primary-in ... | 2015 | 25903558 |
| draft genome of neurotropic nematode parasite angiostrongylus cantonensis, causative agent of human eosinophilic meningitis. | angiostrongylus cantonensis is a bursate nematode parasite that causes eosinophilic meningitis (or meningoencephalitis) in humans in many parts of the world. the genomic data from a. cantonensis will form a useful resource for comparative genomic and chemogenomic studies to aid the development of diagnostics and therapeutics. we have sequenced, assembled and annotated the genome of a. cantonensis. the genome size is estimated to be ∼260 mb, with 17,280 genomic scaffolds, 91x coverage, 81.45% for ... | 2015 | 25910624 |
| comparative immunoprophylactic efficacy of haemonchus contortus recombinant enolase (rhceno) and con a purified native glycoproteins in sheep. | haemonchus contortus is the most economically important blood feeding nematode parasite of sheep and goats all over the world. enolase in helminth parasites is a multi-functional enzyme which involves in glycolysis and host tissue invasion. in this study, the recombinant h. contortus enolase (rhceno) was evaluated for its immunoprophylactic efficacy in sheep along with con a purified native glycoproteins in a vaccine challenge trial. group i and group ii experimental sheep were immunized thrice ... | 2015 | 25913090 |
| screening and analysis of hc-ubq and hc-gst related to desiccation survival of infective haemonchus contortus larvae. | infective haemonchus contortus larvae (l3s) are able to protect themselves from desiccation. to explore the molecular mechanisms of desiccation survival, mrna differential display rt-pcr was used to screen differentially expressed genes in l3s upon desiccation, followed by rnai experiments to define gene functions. in this, 58 differentially expressed transcripts were obtained. among these, the bf-u01a and ch-u02a fragments represent genes with the highest identity percentage in bioinformatic an ... | 2015 | 25913452 |
| comparison of biotransformation and efficacy of aminoacetonitrile anthelmintics in vitro. | the present in vitro study was designed to test and compare anthelmintic activity, hepatotoxicity, and biotransformation of four selected aminoacetonitrile derivatives (aads): monepantel (mop, anthelmintic approved for the treatment), aad-970, aad-1154, and aad-1336. micro-agar larval development test, mtt test of cytotoxicity, and biotransformation study coupled with ultra high performance liquid chromatography-tandem mass spectrometry (uhplc-ms/ms) technique were used for this purpose. larvae ... | 2016 | 25922167 |
| heterologous expression in remodeled c. elegans: a platform for monoaminergic agonist identification and anthelmintic screening. | monoamines, such as 5-ht and tyramine (ta), paralyze both free-living and parasitic nematodes when applied exogenously and serotonergic agonists have been used to clear haemonchus contortus infections in vivo. since nematode cell lines are not available and animal screening options are limited, we have developed a screening platform to identify monoamine receptor agonists. key receptors were expressed heterologously in chimeric, genetically-engineered caenorhabditis elegans, at sites likely to y ... | 2015 | 25928899 |
| ivermectin binds to haemonchus contortus tubulins and promotes stability of microtubules. | haemonchus contortus is a nematode of livestock that can cause severe disease and mortality. ivermectin, an anti-parasitic drug that targets glutamate-gated chloride channels, is widely used in humans, livestock, companion animals and agriculture. although an association between genetic changes to β-tubulin and exposure to ivermectin has been previously reported, direct binding between ivermectin and tubulin has not been demonstrated to date. tubulin/microtubules are key targets for many anti-mi ... | 2015 | 25957159 |
| partial protection and abomasal cytokine expression in sheep experimentally infected with haemonchus contortus and pre-treated with taenia hydatigena vesicular concentrate. | the abomasal expression of il-2, il-4, il-6, il-10 and ifnγ in lambs experimentally infected with haemonchus contortus and its relationship to protection induced by a taenia hydatigena larvae vesicular concentrate (thlvc) were evaluated. the lambs that were only infected with h. contortus larvae showed a worm burden greater (p<0.05) than the lambs that received thlvc prior to infection. moreover, the lambs that received thlvc showed a greater (p<0.05) number of blood eosinophils than the lambs t ... | 2015 | 25959643 |
| in vitro levamisole selection pressure on larval stages of haemonchus contortus over nine generations gives rise to drug resistance and target site gene expression changes specific to the early larval stages only. | there is some evidence that resistance to levamisole and pyrantel in trichostrongylid nematodes is due to changes in the composition of nicotinic acetylcholine receptors (nachrs) which represent the drug target site. altered expression patterns of genes coding for nachr subunits, as well as the presence of truncated versions of several subunits, have been implicated in observed resistances. the studies have mostly compared target sites in worm isolates of very different genetic background, and h ... | 2015 | 25983232 |
| serum interleukin-4 (il-4) production is associated with lower fecal egg count in parasite-resistant sheep. | the objective of this experiment was to determine serum interleukin-4 (il-4) concentration in response to haemonchus contortus infection in parasite-resistant and -susceptible lambs. st. croix (stc) (resistant) and suffolk/hampshire crossbred (sx) (susceptible) lambs were either not infected (n=5/breed), given a primary inoculation (n=5/breed) or challenged infected with h. contortus (n=5/breed). each inoculum given consisted of 10,000 l3 larvae. blood was collected daily for 14 days and then we ... | 2015 | 25986324 |
| effects of third generation p-glycoprotein inhibitors on the sensitivity of drug-resistant and -susceptible isolates of haemonchus contortus to anthelmintics in vitro. | p-glycoproteins (p-gps) play an important role in the sensitivity of nematodes to anthelmintic drugs. they have been implicated in a number of anthelmintic resistances, particularly for macrocyclic lactone drugs. hence, inhibition of nematode p-gps has been suggested as a means of reversing some types of anthelmintic resistance. the present study aimed to investigate the ability of the most-recently developed group of p-gp inhibitors (the so-called 'third generation' of inhibitors) including tar ... | 2015 | 25986327 |
| synthesis of photoreactive ivermectin b1a derivatives and their actions on haemonchus and bombyx glutamate-gated chloride channels. | glutamate-gated chloride channels (glucls) are inhibitory neurotransmitter receptors that are present only in invertebrates such as nematodes and insects. these channels are important targets of insecticidal, acaricidal, and anthelmintic macrolides such as avermectins, ivermectin (ivm), and milbemycins. to identify the amino acid residues that interact with ivm in glucls, three ivm b1a derivatives with different photoreactive substitutions at c-13 were synthesized in the present study. these der ... | 2015 | 25987225 |
| vaccination of lambs with the recombinant protein rhc23 elicits significant protection against haemonchus contortus challenge. | gene encoding a somatic protein of haemonchus contortus (hc23) known to confer significant protection against experimental haemonchosis has been cloned and expressed in a prokaryotic system. a cdna library of h. contortus using the vector λ zap ii was obtained. full-length gene was amplified, cloned and expressed in escherichia coli bl21. the recombinant protein was purified in ni-nta column. recombinant protein (rhc23) had 203 aminoacids and a molecular mass of 24.15 kda. recombinant protein (1 ... | 2015 | 25997645 |
| the prevalence of parasitic infestation of small ruminant farms in perak, malaysia. | helminthiasis due to strongyles such as haemonchus contortus, coccidiosis caused by eimeria sp. and blood parasite diseases such as theileriosis by theileria sp. have been reported to cause severe morbidity and mortality annually in small ruminants in malaysia. the aims of this study were to investigate the prevalence of helminthiasis, coccidiosis and theileriosis and to determine the packed cell volume (pcv) value of small ruminants in perak, malaysia. blood and faecal samples were obtained fro ... | 2015 | 26019746 |
| anthelmintic efficacy of aqueous extract of butea monosperma (lam.) kuntze against haemonchus contortus of sheep and goats. | infection with haemonchus contortus is one of the most important economic problems in small ruminants worldwide. resistance development by parasites, drug residues in meat, toxicity, non-availability and high cost limit the usefulness of currently used synthetic drugs. therefore, the present study was undertaken to evaluate in vitro anthelmintic efficacy of aqueous extract of seeds of butea monosperma (lam.) kuntze against h. contortus. phytochemical analysis of extract showed high concentration ... | 2015 | 26064000 |
| survey on anthelmintic resistance to gastrointestinal nematodes in unorganized goat farms of tamil nadu. | one of the major problems in small ruminant farms is emergence of anthelmintic resistance (ar) to commonly used dewormers. this study investigated ar to gastrointestinal nematodes affecting goats in 27 unorganized farms in three different agro-climatic zones (cauvery delta zone, high altitude zone and high rainfall zone) of tamil nadu, india. two anthelmintics viz., albendazole (az) and levamisole (lev) were used in this study as per the dose recommended by the manufacturer. status of ar was det ... | 2015 | 26064012 |
| anthelmintic activities against haemonchus contortus or trichostrongylus colubriformis from small ruminants are influenced by structural features of condensed tannins. | plants containing condensed tannins (cts) may hold promise as alternatives to synthetic anthelmintic (ah) drugs for controlling gastrointestinal nematodes (gins). however, the structural features that contribute to the ah activities of cts remain elusive. this study probed the relationships between ct structures and their ah activities. eighteen plant resources were selected on the basis of their diverse ct structures. from each plant resource, two ct fractions were isolated and their in vitro a ... | 2015 | 26066999 |
| in vitro anthelmintic activity of zanthoxylum simulans essential oil against haemonchus contortus. | the need for new anthelmintic agents with low impact on the environment is becoming urgent. phytotherapy is an alternative method to control gastrointestinal nematodes in small ruminants. this study aims to determine the composition of zanthoxylum simulans essential oil (zseo) and evaluate the in vitro ovicidal and larvicidal effects of zseo on haemonchus contortus using egg hatch assay, larval development assay (lda), and larval migration inhibition assay (lmia). the chemical composition of zse ... | 2015 | 26073109 |
| development of a multiplex fluorescence immunological assay for the simultaneous detection of antibodies against cooperia oncophora, dictyocaulus viviparus and fasciola hepatica in cattle. | a major constraint for the effective control and management of helminth parasites is the lack of rapid, high-throughput, routine diagnostic tests to assess the health status of individual animals and herds and to identify the parasite species responsible for these helminthoses. the capability of a multiplex platform for the simultaneous detection of three pasture associated parasite species was evaluated and compared to existing elisas. | 2015 | 26084663 |
| plants of the cerrado naturally selected by grazing sheep may have potential for inhibiting development of haemonchus contortus larva. | plant species naturally selected by sheep grazing in the cerrado region of brazil were assessed in vitro for activity against haemonchus contortus. one year of observations showed the plant families in the region exhibiting greatest richness to be fabaceae, rubiaceae, malpighiaceae, bignoniaceae, myrtaceae, and annonaceae. nine species commonly selected by grazing sheep showed variation in the selectivity index with respect to the dry and rainy seasons. coproculture was conducted in five replica ... | 2015 | 26085457 |
| relative expression analysis of il-5 and il-6 genes in tropical sheep breed pelibuey infected with haemonchus contortus. | haemonchus contortus is a parasitic nematode of pelibuey sheep, a meat breed used in tropical regions. due to anthelmintic problems, the identification of hosts resistant to h. contortus is another option of control. the aim of this study was to analyse the relative expression of il-5 and il-6 genes in pelibuey sheep after h. contortus infection. nineteen lambs infected with h. contortus and three more lambs without infection were studied. the haemonchosis was determined by the number of eggs pe ... | 2015 | 26094646 |
| in vitro activity of essential oils of free and nanostructured melaleuca alternifolia and of terpinen-4-ol on eggs and larvae of haemonchus contortus. | haemonchus contortus is one of the major gastrointestinal nematodes responsible for significant economic and production losses of sheep. diseases caused by this species lack effective anthelmintic products, and the search for new compounds to replace synthetic anthelmintics has been extensive. the present investigation assesses the in vitro activity of the essential oil of melaleuca (melaleuca alternifolia), both free (tto) and nanostructured (nanotto), and terpinen-4-ol (terp-4-ol) on eggs and ... | 2016 | 26096177 |
| genetic evidence for the spread of a benzimidazole resistance mutation across southern india from a single origin in the parasitic nematode haemonchus contortus. | it is important to understand how anthelmintic drug resistance mutations arise and spread in order to determine appropriate mitigation strategies. we hypothesised that a molecular genetic study of haemonchus contortus in southern india, a region where resistance may be less advanced than in western europe and north america, might provide some important insights into the origin and spread of anthelmintic resistance. the f200y (tac) isotype-1 β-tubulin benzimidazole resistance mutation is common i ... | 2015 | 26099649 |
| a perspective on genomic-guided anthelmintic discovery and repurposing using haemonchus contortus. | high-throughput molecular and computer technologies have become instrumental for systems biological explorations of parasites. investigating the genomes and transcriptomes of different developmental stages of parasitic nematodes can provide insights into gene expression, regulation and function in the parasite, which is a significant step toward understanding their biology as well as host interactions and disease. this article covers aspects of a talk given at the meegid xii conference in thaila ... | 2016 | 26144657 |
| anthelmintic activity of artemisia vestita wall ex dc. and artemisia maritima l. against haemonchus contortus from sheep. | current study was designed to evaluate in vivo and in vitro anthelmintic activity of artemisia vestita wall ex dc. and artemisia maritima l. against haemonchus contortus in comparison with ivermectin to investigate the effect of plant extracts on survival of infective l3 and adults under in vitro condition. plant extracts were given to h. contortus infected sheep orally and it was infected with l3 stage of h. contortus at dose of 5000 larvae/sheep. total of 25-30 larvae were incubated with plant ... | 2015 | 26194429 |
| synergistic inhibition of haemonchus contortus exsheathment by flavonoid monomers and condensed tannins. | this study investigated the separate and combined anthelmintic (ah) effects of different phenolic compounds, including condensed tannins and flavonoids, all of which are known to occur in willow leaves, a potentially valuable dry season feed. a range of contrasting model tannins, which span the whole range of willow tannins, were isolated from tilia flowers, goat willow leaves, black currant leaves and red currant leaves. all together, the tested compounds represented the major tannin types (pro ... | 2015 | 26199861 |
| galectin-11: a novel host mediator targeting specific stages of the gastrointestinal nematode parasite, haemonchus contortus. | galectin-11 is released from epithelial cells of the gastrointestinal tract, specifically following infection with gastrointestinal parasites including the highly pathogenic nematode, haemonchus contortus. the function(s) of galectin-11 are currently unknown but seem to be associated with the development of immunity by the host. the aim of the present study was to examine the interaction of galectin-11 with the different parasitic life cycle stages of h. contortus and determine any effects on pa ... | 2015 | 26215057 |
| effects of peripheral blood mononuclear cells on haemonchus contortus larval motility in vitro. | the objective of this experiment was to determine effects of peripheral blood mononuclear cells (pbmc), derived from parasite-resistant st. croix (stc) and parasite-susceptible suffolk (sf) sheep, on motility of haemonchus contortus l3 stage larvae in vitro. peripheral blood mononuclear cells were collected from 10 lambs of each breed, 5 naïve and 5 which had received a priming infection with h. contortus. larval motility was quantified using a mif nikon sweptfield microscope and nis elements ar ... | 2015 | 26223339 |
| in vitro effects of eucalyptus staigeriana nanoemulsion on haemonchus contortus and toxicity in rodents. | strategies for controlling gastrointestinal nematodes have been developed based on the use of numerous alternative methods, including the use of phytotherapy. new formulations of essential oils with anthelmintic activity have been proposed as a means to optimize their biological effects. thus, the objective of this study was to formulate a nanoemulsion to optimize the nematicide effect of eucalyptus staigeriana essential oil (eseo). initially, physico-chemical analyses were performed to verify t ... | 2015 | 26233731 |
| the barber's pole worm cap protein superfamily--a basis for fundamental discovery and biotechnology advances. | parasitic worm proteins that belong to the cysteine-rich secretory proteins, antigen 5 and pathogenesis-related 1 (cap) superfamily are proposed to play key roles in the infection process and the modulation of immune responses in host animals. however, there is limited information on these proteins for most socio-economically important worms. here, we review the cap protein superfamily of haemonchus contortus (barber's pole worm), a highly significant parasitic roundworm (order strongylida) of s ... | 2015 | 26239368 |
| influence of immunoprotection on genetic variability of cysteine proteinases from haemonchus contortus adult worms. | the limitations associated with the use of anthelmintic drugs in the control of gastrotintestinal nematodosis, such as the emergence of anthelmintic resistance, have stimulated the study of the immunological control of many parasites. in the case of haemonchus contortus, several vaccination trials using native and recombinant antigens have been conducted. a group of antigens with demonstrated immunoprotective value are cathepsin b - like proteolytic enzymes of the cysteine proteinase type. these ... | 2015 | 26241655 |
| macrocyclic lactones and their relationship to the snps related to benzimidazole resistance. | haemonchus contortus is an abomasal nematode of ruminants that is widely present across the world. its ability to cause death of infected animals and rapidly develop anthelmintic resistance makes it a dangerous pathogen. ivermectin (ivm) and moxidectin (mox) are macrocyclic lactones (mls). they have been successfully used to treat parasitic nematodes over the last three decades. a genetic association between ivm selection and single nucleotide polymorphisms (snps) on the β-tubulin isotype 1 gene ... | 2015 | 26253426 |
| reliable reference gene selection for quantitative real time pcr in haemonchus contortus. | the aim of this work was to identify reliable reference genes for expression studies in adult haemonchus contortus. eleven candidate genes were identified and the stability of their expression was assessed in adult males and females of two genetically divergent h. contortus isolates: drug-susceptible (ise) and multi-drug-resistant (wr). five genes with the most stable expression patterns were further assessed for suitability as reference genes in anthelmintic-treated h. contortus adults versus n ... | 2015 | 26255779 |
| inhibition of haemonchus contortus larval development by fungal lectins. | lectins are carbohydrate-binding proteins that are involved in fundamental intra- and extracellular biological processes. they occur ubiquitously in nature and are especially abundant in plants and fungi. it has been well established that certain higher fungi produce lectins in their fruiting bodies and/or sclerotia as a part of their natural resistance against free-living fungivorous nematodes and other pests. despite relatively high diversity of the glycan structures in nature, many of the gly ... | 2015 | 26283415 |
| the n-terminal segment of glyceraldehyde-3-phosphate dehydrogenase of haemonchus contortus interacts with complements c1q and c3. | haemonchus contortus, an economically important blood-sucking parasite of sheep and goats, survives the harsh host gut environment by secreting a number of proteins referred as excretory/secretory (es) products. glyceraldehyde-3-phosphate dehydrogenase (gapdh), a glycolytic enzyme, is one of the components of h. contortus es products. the parasite enzyme binds to complement c3 and inhibits its activity. in this study, the c3-binding activity of the parasite gapdh was mapped to the n-terminal par ... | 2015 | 26332726 |
| dried, ground banana plant leaves (musa spp.) for the control of haemonchus contortus and trichostrongylus colubriformis infections in sheep. | to evaluate the anthelmintic effect of musa spp. leaves, 12 animals were artificially infected with haemonchus contortus, and another 12 animals were infected with trichostrongylus colubriformis. then, both treatment groups were offered 400 g of dried ground banana plant leaves, and the control animals were offered only 1000 g of coast cross hay. during the trials, the animals received weekly physical examinations. the methods used to evaluate the efficiency of this treatment were packed cell vo ... | 2015 | 26350377 |
| the effects of diet and corticosteroid-induced immune suppression during infection by haemonchus contortus in lambs. | to evaluate the effects of diet and corticosteroid-induced immune suppression during infection by haemonchus contortus, 28 lambs were allocated to one of four groups treated as follows: group basal diet - normal; group basal diet - immune-suppressed; group supplemented diet - normal; and group supplemented diet - immune-suppressed. the basal diet contained cynodon dactylon (cv. coast cross) hay with 82 g crude protein (cp)/kg dry matter (dm), which was provided to the lambs in all groups ad libi ... | 2015 | 26391820 |
| proteolytic activity of extracellular products from arthrobotrys musiformis and their effect in vitro against haemonchus contortus infective larvae. | arthrobotrys musiformis is a nematophagous fungus with potential for the biological control of haemonchus contortus larvae. this study aimed to identify and demonstrate the proteolytic activity of extracellular products from a musiformis cultured in a liquid medium against h contortus infective larvae. a musiformis was cultured on a solid medium and further grown in a liquid medium, which was then processed through ion exchange and hydrophobic interaction chromatography. the proteolytic activity ... | 2015 | 26392902 |
| targeting lysine deacetylases (kdacs) in parasites. | due to an increasing problem of drug resistance among almost all parasites species ranging from protists to worms, there is an urgent need to explore new drug targets and their inhibitors to provide new and effective parasitic therapeutics. in this regard, there is growing interest in exploring known drug leads of human epigenetic enzymes as potential starting points to develop novel treatments for parasitic diseases. this approach of repurposing (starting with validated targets and inhibitors) ... | 2015 | 26402733 |
| oxidative stress by haemonchus contortus in lambs: influence of treatment with zinc edetate. | the aim of the present study was to assess the effects of zinc edetate on the oxidative stress of lambs infected by haemonchus contortus. twenty-four lambs were allocated into four groups: group i--uninfected animals; group ii--uninfected animals treated subcutaneously with zinc edetate; group iii--animals infected by h. contortus and group iv--animals infected and treated. the oxidative stress index (osi) and the eggs per gram of feces (epg) were assessed after 10, 17, 24, 31 and 38 days post-i ... | 2015 | 26412513 |
| expression of serine proteinase p186 of arthrobotrys oligospora and analysis of its nematode-degrading activity. | the nematode-trapping fungi possess a unique capability of predating and invading nematodes. as a representative nematode-trapping fungus, arthrobotrys oligospora has been widely used to study the interactions between nematode-trapping fungi and their hosts. serine proteinase is one of the important virulence factors during process of invasion of the nematode-trapping fungi into nematodes. in this study, using reverse transcription polymerase chain reaction, we amplified the gene sequence of ser ... | 2015 | 26419902 |
| acquired resistance to monepantel in c. elegans: what about parasitic nematodes? | in 2008, novartis animal health developed a new class of anthelmintics, the amino-acetonitrile derivatives (aad) of which monepantel is the most prominent compound. monepantel was designed for the treatment of sheep against the parasitic nematode haemonchus contortus. because monepantel acts through a different mechanism, it is effective against nematodes that have acquired resistance to long-standing anthelmintics. in order to benefit from a maximum lifespan and efficacy of this new compound, t ... | 2017 | 26430545 |
| anthelmintic activity of cymbopogon citratus against haemonchus contortus. | parasitic nematodes are of major economic importance in livestock. an alternative for the control of parasites is phytotherapy. this study evaluated the efficacy of cymbopogon citratus decoction (ccd), c. citratus essential oil (cceo) and citral against haemonchus contortus using in vitro egg hatch test (eht) and larval development test (ldt) and an in vivo test using a meriones unguiculatus (gerbil) model. the effect of 800 mg/kg cceo was evaluated in gerbils that had been artificially infected ... | 2015 | 26444058 |
| galectins and collectinis expression are increased in haemonchus contortus-infected corriedale sheep. | galectins and collectins are proteins classified in the lectin family that have the ability to recognize molecular patterns associated with pathogens. studies on cattle have demonstrated high expression of these proteins during infection with gastrointestinal nematodes. the aim of this study was to investigate whether the level of haemonchus contortus infection would alter the expression of galectins (gal11 and gal14) and collectins (spa and cgn) in sheep. twelve corriedale sheep exposed to natu ... | 2015 | 26444062 |
| immunomodulatory effects of adult haemonchus contortus excretory/secretory products on human monocyte-derived dendritic cells. | the levels of expression of surface molecules and release of cytokines and chemokines of human monocyte-derived dendritic cells were determined after their exposure to adult h. contortus excretory/secretory (es) products or a combination of es products and bacterial lipopolysaccharide (lps). worm products provoked a weak response and only partial maturation of the dendritic cells, consistent with the hyporesponsiveness and more tolerogenic immune environment present in parasitized animals and hu ... | 2015 | 26457886 |
| climate-driven changes to the spatio-temporal distribution of the parasitic nematode, haemonchus contortus, in sheep in europe. | recent climate change has resulted in changes to the phenology and distribution of invertebrates worldwide. where invertebrates are associated with disease, climate variability and changes in climate may also affect the spatio-temporal dynamics of disease. due to its significant impact on sheep production and welfare, the recent increase in diagnoses of ovine haemonchosis caused by the nematode haemonchus contortus in some temperate regions is particularly concerning. this study is the first to ... | 2016 | 26482823 |
| livestock helminths in a changing climate: approaches and restrictions to meaningful predictions. | climate change is a driving force for livestock parasite risk. this is especially true for helminths including the nematodes haemonchus contortus, teladorsagia circumcincta, nematodirus battus, and the trematode fasciola hepatica, since survival and development of free-living stages is chiefly affected by temperature and moisture. the paucity of long term predictions of helminth risk under climate change has driven us to explore optimal modelling approaches and identify current bottlenecks to ge ... | 2012 | 26486780 |
| fungal antagonism assessment of predatory species and producers metabolites and their effectiveness on haemonchus contortus infective larvae. | the objective of this study was to assess antagonism of nematophagous fungi and species producers metabolites and their effectiveness on haemonchus contortus infective larvae (l3). assay a assesses the synergistic, additive, or antagonistic effect on the production of spores of fungal isolates of the species duddingtonia flagrans, clonostachys rosea, trichoderma esau, and arthrobotrys musiformis; assay b evaluates in vitro the effect of intercropping of these isolates grown in 2% water-agar (2% ... | 2015 | 26504791 |
| efficacy of clonostachys rosea and duddingtonia flagrans in reducing the haemonchus contortus infective larvae. | the biocontrol is proven effective in reducing in vitro and in situ free-living stages of major gastrointestinal helminths, allowing progress in reducing losses by parasitism, maximizing production, and productivity. this study aimed at evaluating the predatory activity of fungal isolates of duddingtonia flagrans and clonostachys rosea species and its association on infective larvae (l3) of h. contortus in microplots formed by grasses and maintained in a protected environment. all groups were ad ... | 2015 | 26504809 |
| real-time pcr/dna melting curve-based assay to identify individual strongylid larvae recovered from ovine faecal cultures. | a closed-tube real-time pcr (rt pcr) method was developed to identify individual strongylid nematode larvae recovered from ovine faecal cultures. the method builds on an earlier conventional pcr assay established by our group and similarly targets species-specific sequence motifs in the its-2 region of ribosomal dna. the new procedure combines rt pcr with dna melting curve analyses to identify species-specific amplicons, thus avoiding the need to undertake gel electrophoresis. as with the earlie ... | 2015 | 26526096 |
| effect of selective anthelmintic treatments on health and production parameters in pelibuey ewes during lactation. | a study was conducted from december to april 2013, with the aim of evaluating a system of selective antiparasitic treatments using the famacha© color chart compared with a conventional suppressive deworming system every 30 days in pelibuey ewes during lactation. for the study, 54 ewes were used. they were randomly divided into two groups: famacha and chemical treatments. the ewes in the first group received selective treatment depending on the ocular mucosa coloration (famacha) and body conditio ... | 2016 | 26563269 |
| effectiveness evaluation of levamisole, albendazole, ivermectin, and vernonia amygdalina in west african dwarf goats. | anthelmintic drug resistance has led to the search for alternatives in controlling helminth infections. fifty west african dwarf goats without history of anthelmintic treatment were divided equally into five groups. group a was treated with ivermectin injection subcutaneously, group b with levamisole subcutaneously, group c with albendazole orally, and group d with aqueous extract of vernonia amygdalina and group e was untreated control. faecal samples were collected before treatment from each a ... | 2015 | 26579232 |
| the complement of family m1 aminopeptidases of haemonchus contortus--biotechnological implications. | although substantial research has been focused on the 'hidden antigen' h11 of haemonchus contortus as a vaccine against haemonchosis in small ruminants, little is know about this and related aminopeptidases. in the present article, we reviewed genomic and transcriptomic data sets to define, for the first time, the complement of aminopeptidases (designated hc-ap-1 to hc-ap-13) of the family m1 with homologues in caenorhabditis elegans, characterised by zinc-binding (hexxh) and exo-peptidase (game ... | 2016 | 26597954 |
| leucocyte-derived extracellular trap formation significantly contributes to haemonchus contortus larval entrapment. | polymorphonuclear neutrophil (pmn) and eosinophil extracellular trap (ets) formation has recently been described as an important host effector mechanism against invading pathogens. so far, scarce evidence on metazoan-triggered et formation has been published. we here describe for the first time haemonchus contortus-triggered ets being released by bovine pmn and ovine eosinophils in response to ensheathed and exsheathed third stage larvae (l3). | 2015 | 26610335 |
| target selected treatment with levamisole to control the development of anthelmintic resistance in a sheep flock. | levamisole phosphate, chosen based on its 100 % efficacy demonstrated by a previous fecal egg count reduction test (fecrt), was used as the exclusive anthelmintic treatment in the embrapa southeast livestock sheep flock from 2009 to 2014 in a target selected treatment scheme. in the present study, the effectiveness of this nematode control scheme was evaluated after 5 years by fecrt, larval development test (ldt), and a molecular test to assess the development of levamisole resistance in haemonc ... | 2016 | 26614360 |
| early detection of haemonchus contortus infection in sheep using three different faecal occult blood tests. | haemonchus contortus is a blood-sucking parasite causing the presence of faecal occult blood (fob). the objective was to study three different fob tests in order to have a new indicator of h. contortus infection in sheep that could be included in the genetic evaluation system as an alternative selection criterion to faecal worm egg count (fec). a total of 29 corriedale lambs were experimentally infected with 10.000 larvae of h. contortus. stool samples were recorded for fec and fob tests (hexago ... | 2015 | 26623372 |
| functional characterization of a novel class of morantel-sensitive acetylcholine receptors in nematodes. | acetylcholine receptors are pentameric ligand-gated channels involved in excitatory neuro-transmission in both vertebrates and invertebrates. in nematodes, they represent major targets for cholinergic agonist or antagonist anthelmintic drugs. despite the large diversity of acetylcholine-receptor subunit genes present in nematodes, only a few receptor subtypes have been characterized so far. interestingly, parasitic nematodes affecting human or animal health possess two closely related members of ... | 2015 | 26625142 |
| the haemonchus contortus kinome--a resource for fundamental molecular investigations and drug discovery. | protein kinases regulate a plethora of essential signalling and other biological pathways in all eukaryotic organisms, but very little is known about them in most parasitic nematodes. | 2015 | 26644012 |
| characterization of haemonchus contortus p-glycoprotein-16 and its interaction with the macrocyclic lactone anthelmintics. | anthelmintic resistance in veterinary nematodes, including haemonchus contortus, has become a limitation to maintaining high standards of animal health. resistance in this parasite, to all drug families including the macrocyclic lactones (mls) is a serious issue worldwide. mechanisms of resistance to the mls appear to be complex and to include the elimination of these compounds by abc transporter-like proteins present in nematodes. in order to investigate the potential involvement of abc transpo ... | 2015 | 26657092 |
| in vitro anthelmintic efficacy of native plants against haemonchus contortus. | the current study aimed to investigate in vitro anthelmintic efficacy of two medicinally important plants against haemonchus contortus in small ruminants. fruit peel of punica granatum linn. (vern. anar), leaves and roots of berberis lycium royle (vern. sumbal) were tested for their anthelmintic efficacy. methanolic extracts of the test plants from various plant parts were tested for anthelmintic efficacy against the haemonchus contortous using albendazole as a reference standard. the results re ... | 2015 | 26665413 |
| protein profiling of haemonchus contortus found in sheep of kashmir valley. | economic losses due to helminth parasites in sheep throughout the world are considerable. haemonchus contortus is a blood sucking intestinal helminth that lives in the abomasum of small ruminants worldwide. this parasite can be devastating to producers as it causes decreased production levels due to clinical signs such as anaemia, edema and death. for isolation of the proteins of the parasite, a well defined methodology was adopted. the abomasae of sheep in which this parasite resides were colle ... | 2015 | 26688626 |
| anthelmintic activity of leucaena leucocephala protein extracts on haemonchus contortus. | the objective of this study was to evaluate the effects of protein extracts obtained from the plant leucaena leucocephala on the nematode parasite haemonchus contortus. the seeds, shell and cotyledon of l. leucocephala were separated and their proteins extracted using a sodium phosphate buffer, and named as te (total seed extract), se (shell extract) and ce (cotyledon extract). soluble protein content, protease, protease inhibitory and chitinase activity assays were performed. exsheathment inhib ... | 2015 | 26689178 |
| larvicidal activity of bacillus circulans against the gastrointestinal nematode haemonchus contortus in sheep. | efficient control of gastrointestinal parasites is necessary in sheep breeding. however, the available chemically based anthelmintics are becoming less effective due to the development of parasite resistance. an alternative to this problem is biological control. in the present study, we tested the larvicidal effect of bacillus circulans by administering a spore suspension (2 × 109 colony forming units/ml) orally to lambs naturally infected with haemonchus contortus. the number of faecal larvae w ... | 2016 | 26693886 |
| comparative response of the west african dwarf goats to experimental infections with red sokoto and west african dwarf goat isolates of haemonchus contortus. | response of the west african dwarf (wad) goats to two different isolates of haemonchus contortus, the red sokoto (rs) goat isolate (rshc) and the wad goat isolate (wadhc) (isolated from wad goats), was studied by experimental infections of 4-6-month-old male wad goat kids. group 1 and group 2 goats were each infected with 4500 infective larvae (l3) of rshc and wadhc, respectively. group 3 animals served as uninfected control. prepatent period (ppp), faecal egg counts (fec), worm burden (wb), bod ... | 2015 | 26697224 |
| [a comparative analysis of various antigenic proteins found in haemonchus contortus--a review]. | many innovative researches on the development and introduction of recombinant vaccines against many economically important parasites were carried out in the 20th century. research continues to hold promise with the development of immunological and molecular approaches for control of these parasites and in this regard it has already been seen that blood-sucking parasites such as haemonchus contortus and ostertagia ostertagi are susceptible to control by vaccines containing "novel" or "concealed" ... | 2015 | 26710767 |