Publications

comparative analysis of larval excretory-secretory antigens of baylisascaris procyonis, toxocara canis and ascaris suum by western blotting and enzyme immunoassay.		1988	3366527
ascaris suum antigens incorporated into liposomes used to stimulate protection to migrating larvae.	four- to 8-week-old spf pigs were immunized, using antigens of ascaris suum incorporated into liposomes, via intestinal cannula or orally. avridine was also incorporated in the liposomes in one experiment and interleukin-2 (il-2) injected into pigs in another experiment. a priming dose of embryonate eggs (80-470 eggs/pig) were given in four of six experiments. compared to control animals, the greatest protection of pigs to migrating ascarid larvae from a challenge dose of 10,000 embryonated eggs ...	1988	3347994
ascaris suum and onchocerca volvulus: s-adenosylmethionine decarboxylase.	putrescine-dependent s-adenosylmethionine decarboxylase (ec 4.1.1.50) was demonstrated in ascaris suum and onchocerca volvulus; activation was found to be about fourfold by putrescine. mg2+ did not affect the enzyme activity. a. suum was taken as a model nematode and its s-adenosylmethionine decarboxylase was partially purified and characterized. the molecular weight was estimated to be 220,000. the apparent km-value for adenosylmethionine was determined to be 17 microm. methylglyoxal bis(guanyl ...	1988	3350107
superoxide dismutase activity in extracts of specimens of ascaris suum and several analogous tissues in both sexes.	1. the activities of the enzymes superoxide dismutase (ec.1.15.1.1), and catalase (ec.1.11.1.6) in purified extracts of whole ascaris suum adult males and females, and also in several analogous tissues of each sex, were studied. 2. no catalase activity was found in any of the extracts. 3. considerable superoxide dismutase activity was detected in both sexes and the levels of this activity showed sexual differences in the values found in different tissue locations. 4. the sexual organs of both ma ...	1988	3359758
cellular markers of inflammation in the airways of allergic sheep with and without allergen-induced late responses.	allergic sheep respond to inhaled ascaris suum antigen either with an acute bronchoconstriction alone (acute responders, ar) or both an acute and late bronchoconstriction (dual responders, dr). in this study, we determined if: (1) inflammatory cell composition of bronchoalveolar lavage (bal) obtained during the late response differs between dr and ar; (2) the difference in inflammatory cells is dependent on the prechallenge bal cell composition; and (3) drugs that block late airway responses als ...	1988	3202508
a leukotriene and thromboxane inhibitor (sch 37224) blocks antigen-induced immediate and late responses and airway hyperresponsiveness in allergic sheep.	peptide leukotrienes and thromboxane a2 are putative mediators of allergen-induced late responses and allergen-induced airway hyperresponsiveness (ahr), respectively. sch 37224 blocks antigen-induced leukotriene d4 and thromboxane b2 release in guinea pig lung fragments. it also inhibits leukotriene-mediated allergic guinea pig bronchospasm. sch 37224 was, therefore, tested in allergic sheep (n = 6) with documented immediate and late airway responses and ahr to inhaled ascaris suum antigen. for ...	1988	3204511
suppression of ige antibody production by ascaris suum extract: characterization of suppressive component(s).	partial characterization of the suppressive component(s) of a. suum extract that is (are) responsible for damping production of ige antibody to ovalbumin was performed by physical and chemical methods. digestion of the whole extract with trypsin and chymotrypsin completely abolished the suppressive activity. oxidation with sodium metaperiodate or heating at 56 degrees c, however, had no effect. these results indicate that the integrity of heat-stable protein(s) present in the crude extract is es ...	1988	3228633
role of cyclooxygenase products of arachidonic acid metabolism in ascaris antigen-induced bronchoconstriction in sensitized dogs.	the pharmacologic modulation of ascaris allergen-induced bronchoconstriction was investigated in beagle dogs sensitized by experimental infection with ascaris suum ova. ascaris-induced increases in total lung resistance (rl) and falls in dynamic lung compliance (cdyn) were unaffected by pretreatment with an antihistamine (pyrilamine) given alone but were significantly attenuated (p less than .05) by the cyclooxygenase enzyme inhibitor, indomethacin. the combination of pyrilamine and indomethacin ...	1988	3130478
modification of bronchial blood flow during allergic airway responses.	ascaris suum antigen effects on mean airflow resistance (rl) and bronchial arterial blood flow (qbr) were studied in allergic anesthetized sheep with documented airway responses. qbr was measured with electromagnetic flow probes, and supplemental o2 prevented antigen-induced hypoxemia. aerosol challenge with this specific antigen increased rl and qbr significantly. cromolyn sodium aerosol pretreatment prevented antigen-induced increases in rl but not in qbr. intravenous cromolyn, however, preven ...	1988	3136132
isotope partitioning for nad-malic enzyme from ascaris suum confirms a steady-state random kinetic mechanism.	isotope partitioning studies beginning with e.[14c]nad, e.[14c]malate, e.[14c]nad.mg2+, and e.mg.[14c]malate suggest a steady-state random mechanism for the nad-malic enzyme. isotope trapping beginning with e.[14c]nad and with varying concentrations of mg2+ and malate in the chase solution indicates that mg2+ is added in rapid equilibrium and must be added prior to malate for productive ternary complex formation. equal percentage trapping from e.[14c]nad.mg and e.mg.[14c]malate indicates the mec ...	1988	3280016
eosinophilia in ascaris suum-reinfected mice.	the secondary response of eosinophilia has been studied in mice infected with a. suum. in mice infected orally with 1000 a. suum eggs, larvae disappeared from the body within two weeks after infection. the number of peripheral blood eosinophils decreased to the pre-infection level within eight weeks. a typical secondary response of igg antibody production to egg antigen was found after reinfection with 1000 eggs. the number of peripheral blood eosinophils increased more rapidly after reinfection ...	1988	3372980
a unique cytoskeleton associated with crawling in the amoeboid sperm of the nematode, ascaris suum.	nematode sperm extend pseudopods and pull themselves over substrates. they lack an axoneme or the actin and myosins of other types of motile cells, but their pseudopods contain abundant major sperm protein (msp), a family of 14-kd polypeptides found exclusively in male gametes. using high voltage electron microscopy, a unique cytoskeleton was discovered in the pseudopod of in vitro-activated, crawling sperm of the pig intestinal nematode ascaris suum. it consists of 5-10-nm fuzzy fibers organize ...	1989	2910878
passive membrane properties of motorneurons and their role in long-distance signaling in the nematode ascaris.	in the motornervous system of the large parasitic nematode, ascaris suum, the dorsal and ventral nerve cords are connected by a repeating pattern of single identified motorneuron processes, called commissures (stretton et al., 1978). by making microelectrode penetrations of the commissures, we here report the first successful intracellular recordings of nematode neurons. these cells, like muscle cells of ascaris, exhibit resting potentials of approximately -30 to -40 mv. several tests indicate t ...	1989	2918369
survey of internal parasites in western australian pig herds. 1. prevalence.	between september 1982 and march 1984, 101 western australian piggeries with 15 or more sows were surveyed to determine the prevalence of internal parasites and examine the relationship between parasitism and management practices. faecal samples were collected from 20 pigs in 4 age groups in randomly selected piggeries, and examined for the presence of eggs of helminth parasites and protozoan cysts. evidence of nematode parasites was found in 79% of piggeries. sows were more commonly affected th ...	1989	2930392
purification and properties of the cyclic 3',5'-amp-binding protein from the muscle of the nematode ascaris suum.	the cyclic 3',5'-amp-binding protein was isolated from the muscle of ascaris suum and purified to apparent homogeneity. it migrated as a protein with a relative mr 54,000 on electrophoresis under denaturing conditions. on gel filtration columns it was eluted at a volume corresponding to a protein of mr greater than 200,000 under conditions which kept the cyclic 3',5'-amp-binding property intact. the purified catalytic subunit of protein kinase from ascaris and the c subunit of cyclic 3',5'-amp-d ...	1989	2543296
a possible role for paf in allergen-induced late responses: modification by a selective antagonist.	we determined whether platelet-activating factor (paf) plays a role in allergen-induced airway responses by studying the effects of a selective paf antagonist web-2086 on antigen-induced early and late airway responses in allergic sheep. in seven sheep, inhaled ascaris suum produced significant early (282%) and late (176%) increases in specific lung resistance (srl). web-2086 (1 mg/kg iv) given 20 min before antigen challenge did not affect the early response, but the peak late increase in srl w ...	1989	2745300
alterations in ascaris suum larval burdens, eosinophil numbers, and lysophospholipase activity associated with low levels of dietary iron.	the effect of various amounts of dietary iron on the immune response was investigated using balb/canncr/br mice infected with ascaris suum. changes in numbers of larvae, numbers of eosinophils, and levels of lysophospholipase (lpl) activity in lung or liver tissues were analyzed from nonimmune and immunized mice at 2 and 7 days postinfection (pi). various iron diets did not influence the numbers of tissue larvae, eosinophils, or the lpl activity in lungs or livers of nonimmunized mice at various ...	1989	2926596
glycogen synthesis in the obliquely striated muscle of ascaris suum.	a glycogen synthase, designated gs ii, which occurs in a protein/carbohydrate complex has been purified from ascaris suum muscle. the purified gs-ii complex which is eluted from concanavalin-a--sepharose contains proteins with mr 140,000 and 66,000 and a glycoprotein with a carbohydrate/protein mass ratio of 3:1. gs ii activity was totally dependent on glucose 6-phosphate, but exogenous glycogen was not required for polysaccharide synthesis. the gs-ii complex was not phosphorylated by cyclic-amp ...	1989	2506012
effect of gas phase on carbohydrate metabolism in ascaris suum larvae.	the in vitro metabolism of [1-13c]glucose by ascaris suum third and fourth-stage larvae was analyzed under different gas phases using 13c nuclear magnetic resonance spectroscopy (13c-nmr). third-stage larvae (l3) incubated under a gas phase of 85% n2/5% o2/10% co2 produced trace amounts of [13c]succinate, and molted to fourth-stage larvae (l4) between days 3 and 4 in vitro. however, they appeared to arrest as l3s when incubated under air, or 85% n2/5% o2/10% co2 in the presence of 2 mm potassium ...	1989	2509908
effect of beta-adrenergic blockade and sympathetic stimulation on canine bronchial mast cell response to immune degranulation in vivo.	we examined the effect of beta-adrenoceptor blockade and residual alpha-adrenoceptor effects during sympathetic stimulation on mast cell secretion of histamine in 12 natively allergic mongrel dogs. bronchial mast cell response was measured as the arteriovenous difference (avd) in plasma histamine concentration [h] across the bronchus. plasma [h] was determined simultaneously from the azygos outflow tract and femoral artery as a marker of mast cell response prior to and for 90 s after intra-arter ...	1989	2463779
effects of the chitin synthesis inhibitor diflubenzuron on development of ascaris suum and haemonchus contortus.	the potential of the chitin synthesis inhibitor diflubenzuron (dfb) to alter the development of the parasitic nematodes (ascaris suum and haemonchus contortus was investigated. dfb given orally (10 mg kg-1 per day for 30 days) to sheep inoculated with h. contortus infective larvae did not prevent the establishment of adults or affect fecal egg output. however, there was a significant (greater than 90%) decrease in the number of infective larvae recovered from fecal cultures derived from lambs ha ...	1989	2505433
[histopathologic and cytochemical studies of the kidneys of guinea pigs infected with larvae of ascaris suum goeze, 1782].	the material consisted of 20 male guinea pigs of body weight 300 +/- 50 g. fifteen of them were infected per os with a dose of 5000 invasive eggs of a. suum. at the 4th, 7th and 20th days of infection the animals were dissected and sections of kidney were taken for histopathological and histochemical studies. the sections were stained with hematoxylin and eosin. the presence of mucopolisachcarides was detected with the pas method, the activity of acid and alkaline phosphatases--with gomori's met ...	1989	2470207
comparison of collagen gene sequences in ascaris suum and caenorhabditis elegans.	the nucleotide sequences of a 3060-bp fragment containing almost the entire coding sequence of one ascaris suum collagen gene, and a 3019-bp pair fragment containing the 3' end of another a. suum collagen gene have been determined. the polypeptides encoded by these genes show a striking similarity to two caenorhabditis elegans cuticular collagens, both in the position of the triple-helical regions and in the position of cysteine residues. the results of northern blot hybridisation experiments to ...	1989	2482444
[the biological activity of the lipopolysaccharide complexes in ascarids].	lipopolysaccharide complex with chemical structure similar to that of complexes of microbial origin was isolated from ascaris suum by not phenol-aqueous extraction.	1989	2615713
ascaris suum ova-induced bronchoconstriction, eosinophilia, and ige antibody responses in experimentally infected primates did not lead to histamine hyperreactivity.	the hypothesis that active immunization of primates to give airway allergic responses would also confer on them a hyperreactivity to a nonspecific stimulus such as histamine was tested in 29 normal rhesus primates. at 6 wk after immunization, specific primate ige (rast) to ascaris antigen had increased from 0.35 +/- 0.17 to 0.98 +/- 0.35 units/ml x 10(2) (p less than 0.05). histamine released from bronchial alveolar lavage cells in response to antigen increased from 5.4 +/- 0.67 to 24 +/- 1.8ng/ ...	1989	2466426
leukotriene c4 and b4 in bronchoalveolar lavage fluid during biphasic allergic bronchoconstriction in sheep.	to study the contribution of leukotriene c4 (ltc4), leukotriene d4 (ltd4), leukotriene b4 (ltb4), and inflammation to the antigen-induced late bronchial response, these leukotrienes, histamine, and cell differentials were evaluated in bronchoalveolar lavage fluid (balf). immediate and late responses were induced by ascaris suum antigen inhalation in 11 conscious sheep. bronchoalveolar lavage was performed on 3 separate test days, before challenge (control), during the immediate response, and dur ...	1989	2466428
chronological relationships between mediator release and changes in airway dynamics during an ascaris response in sensitive cynomolgus monkeys.	recently identified ascaris suum sensitive cynomolgus monkeys were further characterized to determine if a chronologic relationship existed between mediator release and onset of bronchoconstriction. in these anesthetized ascaris-sensitive monkeys, aerosol antigen challenge of each animal produced rapid and severe bronchoconstriction, as determined by decreases in dynamic lung compliance (-80.2 +/- 4.1%) and airway conductance (-64.5 +/- 13.8%). maximum changes were achieved within 5 min followin ...	1989	2470112
divalent hapten-induced intestinal anaphylaxis in the mouse: uptake and characterization of a bystander protein.	we examined the mucosal barrier function during anaphylaxis induced by the hapten n,n'-di-2,4,dinitrophenyl-lysine (di-dnp-lysine) in bdf1 female mice immunized with dinitrophenylated ascaris suum extract. immunized mice were gavaged with 10 mg or 50 mg of ovalbumin (ova) with or without n,n'-di-2,4,-dnp-lysine (di-dnp-lysine). animals that received di-dnp-lysine underwent anaphylaxis and were observed to have significantly greater serum concentrations of immunoreactive ova (iova) than control m ...	1989	2514138
a patch-clamp study of effects of dihydroavermectin on ascaris muscle.	1. effects of bath-application of the anti-parasitic agent 22,23-dihydroavermectin b1a (dhavm 20 fm to 0.2 microm) on outside-out and inside-out patches from collagenase-treated muscle membranes of ascaris suum were examined. 2. dhavm was tested as a gamma-aminobutyric acid (gaba) agonist by application to outside-out patches. dhavm failed to open characteristic gaba channels even when high concentrations (20 nm to 0.2 microm) were used. these high concentrations of dhavm also failed to potentia ...	1989	2480169
antigen-coated sepharose beads induce airway eosinophilia and airway hyperresponsiveness in cynomolgus monkeys.	a method of inducing sustained airway eosinophilia and airway hyperresponsiveness in primates has been developed. our method utilizes a series of intratracheal instillations of ascaris suum-coated sepharose 4b beads (3 x 10(5] administered once a week for four weeks. five cynomolgus monkeys (macaca fascicularis) demonstrating a naturally occurring skin and respiratory sensitivity to ascaris suum extract were studied. airway cell composition was measured by bronchoalveolar lavage (bal), and airwa ...	1989	2675702
detection of giardia antigen in stool samples by a semi-quantitative enzyme immunoassay (eia) test.	a semi-quantitative enzyme immunoassay (eia) test for the detection of giardia intestinalis antigen in faeces was developed. in order to avoid unspecific reactions due to anticalf serum activity, igg fractions of anti-giardia rabbit and sheep sera were purified from antibovine antibodies by immunoadsorption. faecal specimens tested in the assay were mixed with normal horse serum to avoid unspecific and proteolytic effects of stool components. out of a range of bacterial and parasitic antigens te ...	1989	2685985
[immunoenzymatic evaluation of serum ige in onchocerciasis in chiapas, mexico].	concentrations of total serum ige were measured by an immunoenzymatic assay (phadezym-prist) in 60 mexican onchocerciasis patients. in order to detect ige antibodies against adult onchocerca volvulus antigens, separately six onchocerciasis sera were depleted of ige antibodies by using a mixture of onchocerca gutturosa, ascaris suum and fasciola hepatica antigenic extracts coupled with sepharose 4b. additionally, the sera were incubated with an adult o. volvulus antigenic extract coupled with sep ...	1989	2697095
responses of nfr/n inbred mice to very low-dose infections with ascaris suum.	mice were evaluated for immunological responses and resistance to challenge infections after single infections. when given infections simultaneously with thiabendazole, larval migration to the liver and lungs was blocked but not when mice were given infections only. enlargement of peyer's patches was observed after infection followed by increased numbers of iga-containing cells in the lamina propria of the intestines and increased levels of hepatobiliary iga. ig-containing cells were variable in ...	1989	2707966
an abattoir survey of the prevalence of gastro-intestinal helminths and stephanurus dentatus in pigs in belize.	in an abattoir survey in belize 137 pigs were examined for gastro-intestinal helminths and stephanurus dentatus. hyostrongylus rubidus, physocephalus sexalatus, globocephalus species and trichostrongylus colubriformis were recorded for the first time. ascarops strongylina, ascaris suum, macracanthorynchus hirudinaceus, strongyloides ransomi, trichuris suis, oesophagostomum species and s. dentatus were also found. oesophagostomum species (45%) and s. dentatus (42%) were the most prevalent. the pr ...	1989	2529683
antigenic relationships between the surface-exposed, secreted and somatic materials of the nematode parasites ascaris lumbricoides, ascaris suum, and toxocara canis.	the cosmopolitan nematode parasites ascaris lumbricoides, ascaris suum, and toxocara canis are closely related phylogenetically, and are all pathogenic to man. in the case of the latter, the antigens released by the tissue-invasive parasitic larvae in vitro ('excretory/secretory' or 'es' antigens) are routinely used for serodiagnostic purposes. here we have found, using radioimmunoprecipitation with defined rabbit antiserum, and sds-page, that there is a significant antigenic similarity between ...	1989	2467777
production and distribution of immunoglobulin-bearing cells in the intestine of young pigs infected with ascaris suum.	pigs of 10 days-1 month old were orally infected with eggs of ascaris suum at different rates and inoculation schedules. histological sections from various parts of the small intestines were prepared to observe the production and localization of immunoglobulin-bearing cells. fluorescent antibody and immunoperoxidase staining methods were used to determine the number of igm-, iga- and igg-producing plasma cells in the intestinal lamina propria. significant increases in immunoglobulin-bearing cell ...	1989	2531490
effect of nedocromil sodium on antigen-induced airway responses in allergic sheep.	we studied the effects of nedocromil sodium and sodium cromoglycate on early and late bronchial responses, the airway inflammation associated with the late response to inhaled ascaris suum antigen in allergic sheep in vivo, and the antigen-induced contractile responses of sheep tracheal smooth muscle in vitro. in addition, we examined the effect of nedocromil sodium on the development of antigen-induced airway hyper-responsiveness in this model. pretreatment with either nedocromil sodium or sodi ...	1989	2547569
purification and properties of cyclic-3',5'-gmp-dependent protein kinase from the nematode ascaris suum.	a cyclic-3',5'-gmp-dependent protein kinase was purified 7400-fold from the reproductive tract of female ascarids to a specific activity of 718 nmol min-1 mg-1 (histone as substrate). the yield of the preparation was 25%. the enzyme protein obtained was homogeneous as judged by isoelectrofocusing and polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. the native enzyme behaved as a dimer of two 82-kda subunits in gel permeation chromatography on superose 12. the protein ...	1989	2549874
role of arachidonic acid metabolites in allergen-induced late responses.	the sheep model of allergic airway disease shares many pathophysiological similarities with allergic airway disease in humans. studies performed in this animal model present strong evidence that the release of arachidonic acid metabolites plays an important role in the development of late bronchial responses to antigen challenge. the release of leukotrienes through the lipoxygenase pathway during the acute bronchial obstruction after inhalation of ascaris suum antigen represents the key factor f ...	1989	2557658
effects of combined receptor antagonists of leukotriene d4 (ltd4) and platelet-activating factor (paf) on rhesus airway responses to ltd4, paf and antigen.	rhesus monkeys with ige-mediated asthmatic-type responses to ascaris suum antigen were pretreated with a combination of 2 receptor antagonists to leukotriene d4 (ltd4) and platelet-activating factor (paf). this combination of anti-ltd4 and anti-paf was shown to inhibit airway responses to either ltd4 or paf in separate experiments in these ascaris airway-reactive animals. when the same combination of ltd4 and paf receptor antagonists was used to pretreat the same animal prior to aerosol challeng ...	1989	2542169
exposure to 1 ppm ozone attenuates the immediate antigenic response of canine peripheral airways.	the effect of oxidant exposure on the immediate airway response to immunologic challenge is controversial. we investigated the response of canine peripheral airways to antigen aerosol, 1-3 h and 24 h after a 5-min exposure to 1 ppm ozone. in dogs that were natively sensitive to ascaris suum antigen, resistance to flow through the collateral system (rcs) was measured using the wedged bronchoscope technique. in eight dogs, four sublobar segments of each lung were wedged: two were exposed to ozone ...	1989	2585539
gaba receptors on the somatic muscle cells of the parasitic nematode, ascaris suum: stereoselectivity indicates similarity to a gabaa-type agonist recognition site.	1. the gamma-aminobutyric acid (gaba) receptors on the somatic muscle cells of ascaris, which mediate muscle cell hyperpolarization and relaxation, have been characterized by use of intracellular recording techniques. 2. these receptors are like mammalian gabaa-receptors in that the response is mediated by an increase conductance to chloride ions. the gabaa-mimetic, muscimol, has a relative potency of 0.40 +/- 0.02 (n = 3) compared to gaba. 3. the stereoselectivity of the gaba receptor on ascari ...	1989	2556203
possible mode of action of toltrazuril: studies on two eimeria species and mammalian and ascaris suum enzymes.	the anticoccidial properties of toltrazuril in eimeria falciformis-infected mice were potentiated by the simultaneous application of pyrimethamine, trimethoprim, or sulfadimidine. the same drugs potentiate the effect of toltrazuril by killing e. tenella schizonts in chicken kidney-cell cultures. activities of some enzymes of the respiratory chain, such as succinate-cytochrome c reductase and nadh oxidase and succinate oxidase from mouse liver, were reduced in the presence of toltrazuril. the sam ...	1989	2560189
effect of picumast dihydrochloride on antigen-induced early and late airway responses in allergic sheep.	in this study the effects of picumast dihydrochloride (3,4-dimethyl-7-[4-(4-chlorobenzyl)piperazine-1-yl]propoxycoumarine++ + dihydrochloride) a novel antiallergic agent, on antigen-induced early and late responses in allergic sheep were examined. for all studies picumast dihydrochloride was administered as an aerosol approximately 20 min before airway challenge with ascaris suum antigen. allergic sheep were challenged on four occasions each greater than or equal to 14 days apart: one placebo tr ...	1989	2576357
preexposure to ozone blocks the antigen-induced late asthmatic response of the canine peripheral airways.	the influence of exposure of the airways to ozone on acute allergic responsiveness has been investigated in several species. little is known, however, about the effect of this environmental pollutant on the late asthmatic response (lar) in animals in which it is exhibited. the purpose of this study was to evaluate this effect in the canine peripheral airways and to assess the potential role of mast cells in modulating the effect. a series of experiments on seven mongrel dogs demonstrated that th ...	1989	2585540
ym461, a paf antagonist, blocks antigen-induced late airway responses and airway hyperresponsiveness in allergic sheep.	we examined the effect of an orally active antagonist, ym461, of platelet activating factor (paf) on antigen-induced early and late airway responses and on the development of airway hyperresponsiveness 24 h after challenge in allergic sheep. early and late airway responses were determined by measuring specific lung resistance (srl) before and periodically after challenge. airway responsiveness was determined from the slopes of dose-response curves of srl vs. increasing doses of carbachol aerosol ...	1989	2620695
af1, a sequenced bioactive neuropeptide isolated from the nematode ascaris suum.	an fmrfamide-like neuropeptide, named af1, was isolated from head extracts of the nematode ascaris suum using five steps of hplc. af1 is a heptapeptide with the amino acid sequence lys-asn-glu-phe-ile-arg-phe-nh2. synthetic af1 (10(-9) to 10(-7) m) rapidly and reversibly abolished slow membrane potential oscillations of identified ventral and dorsal inhibitory motoneurons and selectively reduced their input resistances. synaptic transmission was not blocked. in intact ascaris, af1 inhibited loco ...	1989	2627377
[alpha-amylase activity in serum and some organs of guinea pigs with larval ascariasis].	the studies were carried out on 40 guinea pig males weighting about 230 g. the experimental animals were infected with 7000 invasive eggs of ascaris suum. on the 3-rd, 7-th and 10-th day after infection the activity of alpha-amylase was estimated according to caraway's method in serum, liver, pancreas, lungs, kidneys and spleen. the infection of guinea pigs results in increased activity of enzyme in serum and spleen, and decreased in pancreas, liver, kidneys and lungs. on the 3-rd day after infe ...	1989	2633502
nematode parasite infections of domestic pigs in a sub-tropical and high-rainfall area of india.	fourteen hundred and ninety-six domestic pigs, sus scrofa domestica l., from a sub-tropical and high-rainfall area of india were examined over a period of 18 months to ascertain their nematode parasite spectrum. the eleven species recovered, arranged in descending order of prevalence, are as follows: ascaris suum, oesophagostomum dentatum, bourgelatia diducta, stephanurus dentatus, globocephalus connorfilii, physocephalus sexalutus, ascarops dentata, a. strongylina, pseudocruzia orientalis, seta ...	1989	2787072
[trypsin and alpha-amylase activity in the pancreas of guinea pigs. i. the influence of infective dose and duration of infection by ascaris suum goeze, 1782].	the studies were carried out on guinea pig males weighting about 280 g. the animals were infected with 5000 or 20,000 invasive eggs of ascaris suum. on the 3-rd, 7-th, 10-th and 14-th day of invasion the animals were dissected. lungs were weighted, and the number of ascaris larvae was estimated using baermann method. the activity of alpha-amylase (fennel's method) and trypsin (anson's method) were examined in pancreas homogenate. the infection of guinea pigs with a. suum larvae resulted in a dec ...	1989	2633503
the pyruvate dehydrogenase complex from anaerobic mitochondria of the parasitic nematode ascaris suum: stoichiometry of phosphorylation and inactivation.		1989	2634345
carboxypeptidase inhibitors from ascaris suum: the primary structure.	the carboxypeptidase a inhibitor from ascaris suum was isolated from aqueous extracts by affinity chromatography toward immobilized carboxypeptidase a. the amino acid sequence is dqvrkclsdt10dctngekcvq20knkicstive30iqrcekehft40ipcksnndcq50vwahekicn k60lpwgl65 . the carboxypeptidase a inhibitor is not homologous with the chymotrypsin/elastase or trypsin inhibitors from ascaris, but shows homology in a 9-residue internal sequence with the 37/39-residue carboxypeptidase inhibitors from tomato and p ...	1989	2648995
synergistic effect of migrating ascaris larvae and escherichia coli in piglets.	the effect of intestinal flora on the establishment, development and pathogenicity of ascaris suum larvae in piglets (large white breed) was investigated. the infected piglets with ascaris and escherichia coli showed signs of pneumonia, cough with respiratory difficulties initially even though these moderated with time. they lost appetite and showed signs of unthriftiness with loss of weight. the packed cell volume was normal but the differential leucocyte counts of the pigs infected with ascari ...	1989	2656846
effect of fenbendazole and pyrantel tartrate on the induction of protective immunity in pigs naturally or experimentally infected with ascaris suum.	an experiment was conducted with 96 weanling pigs (avg initial wt 18.5 kg) divided into six treatment with two replicates of eight pigs each. pigs in treatments 1, 2 and 3 were penned in outside pens with dirt lots that previously were contaminated with a. suum ova to induce a natural ascaris infection. pigs in treatments 4, 5 and 6 were penned in an open-front building with solid concrete floors and were experimentally infected with 2,000 embryonated a. suum. ova on d 1, 15 and 29 of the experi ...	1989	2722696
airway responses to inhaled ascaris suum antigen in naturally sensitized beagle dogs.	to characterize the airway responses to inhaled ascaris suum antigen in a group of adult beagle dogs with cutaneous ascaris sensitivity, 9 dogs were challenged with aerosolized ascaris antigen and 6 were subsequently challenged 1 to 2 weeks later with aerosolized saline. changes in lung resistance, dynamic lung compliance, and responsiveness to aerosolized histamine were measured during the ensuing 6 hours. inhalation of ascaris antigen produced immediate bronchoconstriction, but no spontaneous ...	1989	2727396
physicochemical characterization and monoclonal and polyclonal antibody recognition of baylisascaris procyonis larval excretory-secretory antigens.	baylisascaris procyonis larval excretory-secretory (es) antigens consisted of complex glycoproteins ranging from 10 kda to over 200 kda as determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis and lectin binding. five monoclonal antibodies (bapr1-bapr5) produced against b. procyonis es antigens were assayed by western blotting with larval es antigens from b. procyonis, baylisascaris melis, baylisascaris transfuga, ascaris suum, and toxocara canis. bapr1 and bapr2 recognized per ...	1989	2668490
succinate-dependent energy generation in ascaris suum mitochondria.	phosphorylation in isolated ascaris suum mitochondria was much greater in the presence of malate than succinate, but, in the absence of added adenine nucleotides, incubations in succinate resulted in substantial elevations in intramitochondrial atp levels. succinate-dependent phosphorylation was stimulated aerobically and this stimulation was due almost entirely to a site i, rotenone-sensitive, phosphorylation. increased substrate level phosphorylation, coupled to propionate formation, or additi ...	1989	2710162
physico-chemical characteristics of superoxide dismutase in ascaris suum.	1. three sod isoenzymes obtained from purified extracts of ascaris suum were characterized. 2. the physico-chemical characteristics studied were: optimum ph, methods of preservation of enzymatic activity, molecular weight, and the u.v. and visible light absorption spectra. 3. the optimum ph for the cu, zn sod i and ii was 10.2 and 10.1 for the mn sod. 4. the extracts retained their levels of activity longer at -70 degrees c, and after lyophilization. the mn sod was more labile than the cu, zn so ...	1989	2721158
evidence for platelet-activating factor secretion during immune activation in dogs.	to elucidate the potential physiological significance of platelet-activating factor (paf) in acute bronchoconstriction, we studied the effect of ascaris suum antigen on the tachyphylactic response to paf in 15 natively allergic mongrel dogs in vivo. active bronchial tension was measured isometrically, and mediator secretion was measured as the arteriovenous difference (avd) in plasma concentration across the lungs. administration of paf into the bronchial artery caused dose-related contraction i ...	1989	2732178
electron-transfer flavoprotein from anaerobic ascaris suum mitochondria and its role in nadh-dependent 2-methyl branched-chain enoyl-coa reduction.	electron-transfer flavoprotein was purified to apparent homogeneity from mitochondria of the parasitic nematode, ascaris suum. the native molecular weight of the enzyme was 70,000, as estimated by gel filtration, and it migrated as two bands with apparent subunit molecular weights of 37,000 and 31,500 during sodium dodecylsulfate polyacrylamide gel electrophoresis. the enzyme exhibited an absorption coefficient for the bound fad of 13.5 mm-1.cm-1 at 436 nm and a protein/flavin (270 nm/436 nm) ra ...	1989	2736251
the effect of an orally active leukotriene (lt) antagonist ym-16638 on antigen-induced early and late airway responses in allergic sheep.	in this study we examined the effects of an orally active leukotriene (lt) antagonist ym-16638 [[5-[[3-(4-acetyl-3-hydroxy-2-propyl-phenoxy)propyl]thio]-1,3,4- thiadiazol-2-yl]thio] acetic acid on antigen-induced early and late responses in allergic sheep. for all studies ym-16638 was administered via intragastric tube 1 h before airway challenge with ascaris suum antigen. six allergic sheep were challenged on four occasions (2 control and 2 drug trials) each greater than or equal to 14 days apa ...	1989	2740413
accurate and efficient rna polymerase iii transcription in a cell-free extract prepared from ascaris suum embryos.	high-speed supernatant (s100) extracts derived from homogenized ascaris suum embryos efficiently transcribe added rna polymerase iii templates including cloned 5s rrna genes of the filarial parasite brugia malayi. several criteria, including two-dimensional rnase t1 oligonucleotide fingerprint analysis, indicate that in vitro transcription is accurately initiated and terminated.	1989	2747746
cytotoxicity of mouse serum and peritoneal fluid for ascaris suum infective larvae.	serum from balb/c mice infected with ascaris suum resulted in 51cr release from a. suum infective larvae during in vitro incubation. peritoneal fluid from infected or noninfected balb/c mice also produced in vitro release of 51cr from a. suum larvae. 51cr release did not occur when anti-a. suum serum or peritoneal fluid from infected or noninfected mice were heat-inactivated. serum and peritoneal fluid from infected or noninfected akr-c5 deficient mice did not generate 51cr release from a. suum ...	1989	2818799
influence of helminth parasite exposure and strategic application of anthelmintics on the development of immunity and growth of swine.	infection of pigs with the intestinal roundworm parasite ascaris suum and strategic application of anthelmintic drugs during the growing phase of development were observed for specific effects on 1) development of immunity in feeder pigs and 2) growth rate during the finishing phase. management treatments included maintenance in a parasite-free concrete environment, maintenance in a concrete environment and inoculation with 1,000 infective a. suum eggs every other day over a 52-d period, and mai ...	1989	2768122
effect of dietary magnesium on ascaris suum infections of mice.	low amounts of dietary magnesium affected the inflammatory tissue response in nonimmunized mice differently than in immunized mice. eosinophil numbers and lpl activity in lung tissue following infection with a. suum larvae were altered by the level of magnesium in the diets of mice. average or higher dietary levels of magnesium resulted in decreased numbers of lung larvae indicating an overall protective effect. increases in eosinophil numbers or lpl activity were not directly related to the num ...	1989	2789853
serotonin receptors in the tissues of adult ascaris suum.	serotonin (5-hydroxytryptamine, 5-ht) receptors in the muscle and intestinal tissues of adult ascaris suum have been investigated. [3h] lysergic acid diethylamide (lsd) exhibited specific and saturable binding to membranes prepared from both intestine and muscle. the intestinal tissue membranes had an equilibrium dissociation constant (kd) of 2.70 nm for lsd and a kd of 2.50 microm for 5-ht. as compared to the intestine, the muscle membranes had comparatively higher affinity for both lsd (kd = 1 ...	1989	2747743
retrovesicular ganglion of the nematode ascaris.	the nematode nervous system is distinguished by the small number and morphological simplicity of its neurons. recently, the shapes and synaptic interactions of each of the 302 neurons in the small free-living nematode, caenorhabditis elegans, have been determined from reconstructions of serial sections by electron microscopy. comparable anatomical studies of the large parasitic nematode ascaris have concentrated on the dorsal and ventral nerve cords where reconstructions of motor neurons by ligh ...	1989	2754041
some aspects of the pharmacology and physiology of the ascaris suum muscle.	out of 11 agonists, including such agents as bradykinin, prostaglandin e2, eledoisin, only acetylcholine (ach) caused the isolated exposed ascaris muscle to contract during treatment for 30 sec and at 10(-7) g/ml (or lower concentrations). the possibility of using this preparation for the identification and quantification of ach in biological tissues and fluids was highlighted. neostigmine, but not physostigmine, augmented ach activity. this suggests that cholinesterases present in the ascaris m ...	1989	2757466
a paf antagonist blocks antigen-induced airway hyperresponsiveness and inflammation in sheep.	we studied the effects of web-2086, a specific antagonist of platelet-activating factor (paf), on the development of antigen-induced airway hyperresponsiveness and inflammation in sheep (n = 8). for these studies, airway responsiveness was determined from slopes of carbachol dose-response curves (drc) performed at base line (prechallenge) and 2 h after ascaris suum antigen challenges in the following three protocols: 1) antigen challenge alone (control trial), 2) web-2086 (1 mg/kg iv) given 30 m ...	1989	2759969
a novel type of putrescine (diamine)-acetylating enzyme from the nematode ascaris suum.	a cytosolic enzyme catalysing the acetylation of the diamines putrescine, cadaverine, 1,3-diaminopropane and 1,6-diaminohexane has been partially purified from reproductive tissue of the intestinal parasitic nematode ascaris suum. the enzyme formed n-acetylated derivatives of the above diamines when incubated in the presence of acetyl-coa. the michaelis constants (km) for the above diamines were 0.25 nm, 0.1 mm, 1.25 mm and 0.4 mm respectively, and the apparent km for acetyl-coa was 7.7 microm. ...	1989	2775189
efficacy of an in-feed ivermectin formulation against gastrointestinal helminths, lungworms, and sarcoptic mites in swine.	the efficacy of ivermectin as an in-feed formulation was evaluated against naturally acquired gastrointestinal helminths, lungworms, and sarcoptic mites (experiment 1; n = 24) and against induced infection with intestinal nematodes (experiment 2; n = 24) in pigs. treatments consisted of ivermectin administered in feed at concentrations calculated to provide 100 or 200 micrograms/kg of body weight/d for 7 days or of nonmedicated feed (controls) for 7 days. at concentration of 100 micrograms of iv ...	1989	2782722
preparation of monoclonal antibody against angiostrongylus cantonensis antigen.	monoclonal antibodies (mab) against a cantonensis were produced through fusion of immunised spleen cells from balb/c mice with ns-1 myeloma cells at a ratio of 10:1. the successful fusion rate on the 3rd day of fusion was 90.1%. ten mab were characterised, six of which were igg1 and the remaining four were igg2a, igg2b, igm and iga respectively. among 6 igg1 mab, four were a. cantonensis-specific, of which three reacted to adult worm antigen only and one reacted to both adult worm and juvenile w ...	1989	2772697
eosinophilia in mouse regulated by multiple factors following repeated stimulations with ascaris eggs into the peritoneal cavity.	repeated injections of viable eggs of ascaris suum into the peritoneal cavity of balb/c mice induced marked peritoneal eosinophilia (9.3 x 10(6) cells/mouse, 52.8%) without infection. extracted antigen (asc) and eggs that had been killed also were able to induce marked eosinophilia; however, their levels were significantly lower (p less than 0.001). this experimental system was able to demonstrate how various multiple factors cumulatively affected the degree of peritoneal eosinophilia. these fac ...	1989	2786507
substrate activation by malate induced by oxalate in the ascaris suum nad-malic enzyme reaction.	substrate activation of the rate of the nad-malic enzyme reaction by malate is obtained in the presence but not in the absence of oxalate. the substrate activation is a result of competition between malate and oxalate for the e.nadh complex, with malate binding to the form of the complex unprotonated at an enzyme group with a pk of 4.9 and oxalate binding preferentially to the protonated form. the off-rate for nadh from the e.nadh complex is completely rate limiting when the group with a pk of 4 ...	1989	2790001
effects of antigen on tracheal circulation and smooth muscle in sheep of different ages.	the effects of ascaris suum antigen on tracheal circulation and tracheal smooth muscle tone were compared in two groups of sheep: the first group was 1 yr old (14 sheep) and the second 5 yr old (8 sheep). cranial tracheal arteries of anesthetized and paralyzed sheep were perfused at constant flow with monitoring of perfusion pressure. tracheal smooth muscle tone was assessed by measuring changes in the external diameter of the cranial trachea. close-arterial injection of antigen (1-20 micrograms ...	1989	2793719
tissue and ultrastructural localization of 5-hydroxytryptamine (serotonin) in the tissues of ascaris suum with energy dispersive x-ray spectrometry of immunoreactive structures.	muscle, hypodermis and gastrointestinal epithelial cells from adult female ascaris lumbricoides var. suum were found to contain serotonin based upon glyoxylic acid induced histofluorescence and indirect immunolabeling with an antiserotonin monoclonal antibody conjugated to protein a-colloidal gold. histofluorescence indicated that muscle-hypodermis and intestinal epithelial cells contained significant concentrations of 5-hydroxytryptamine while fluorescence was absent in the nerve cord and cutic ...	1989	2807715
enhancement of leukotriene c4 release from primate airway macrophages by cellular interactions.	1. cells were obtained from the lungs of macaque monkeys by bronchoalveolar lavage in order to study the role of cellular interactions in the release of leukotriene c4 (ltc4). 2. in normal monkeys, macrophages were the most abundant cell type recovered, whereas in monkeys sensitized with ascaris suum there was an increase in the numbers of eosinophils and mast cells recovered. 3. challenge of cells from both groups of animals with an optimal concentration of opsonized zymosan (opz) resulted in t ...	1989	2819337
prevalence of helminth infections in swine in denmark.	during 1982-1984 the occurrence of helminths in 66 sow herds was determined by examining faecal samples from swine of different age groups. ascaris suum was found in 88% of the herds, while oesophagostomum spp. and trichuris suis were found in 58 and 23% of the herds, respectively. strongyloides ransomi and hyostrongylus rubidus was not identified in any of the herds. the occurrence of both a. suum and oesophagostomum spp. was strongly related to the age of the animals. a. suum was most frequent ...	1989	2815533
effect of calcium antagonist gallopamil on antigen-induced early and late bronchoconstrictor responses in allergic sheep.	calcium channel blockers have been shown to modify acute antigen-induced bronchoconstriction in allergic animals and in some human subjects with bronchial asthma; however, the effects of calcium antagonists on late-phase bronchial responses are not known. in this investigation, we studied the effects of calcium antagonist gallopamil on antigen-induced early- and late-phase bronchial responses in sheep allergic to ascaris suum antigen. in 11 unsedated sheep, specific lung resistance (srl) was mea ...	1989	2930069
saskatchewan. spurious ascaris suum infection in lambs.		1989	17423463
airway hypersensitivity induced by ascaris suum extract in new zealand romney sheep.	sheep from local farms with and without previous exposure to pigs were tested for their skin and airway responses to a commercial ascaris suum antigen. there was an immediate reaction to intradermal injection of the antigen in 90% of 101 sheep. a bronchial provocation test by aerosol of the same antigen was undertaken on 43 of the sheep with a positive skin reaction. about 70% of sheep showed an immediate airway response to the antigen as an aerosol, reflected as a significant increase in airway ...	1990	16031577
ozone increases susceptibility to antigen inhalation in allergic dogs.	to determine whether o3 exposure increased airway responsiveness to antigen inhalation, we studied airway responsiveness to acetylcholine (ach) and ascaris suum antigen (aa) before and after o3 in dogs both sensitive and insensitive to aa. airway responsiveness was assessed by determining the provocative concentration of ach and aa aerosols that increased respiratory resistance (rrs) to twice the base-line value. o3 (3 parts per million) increased airway responsiveness to ach in dogs both sensit ...	1990	2384406
[analysis of antigens defined by anti-anisakis larvae antibodies of ige and igg type in the sera of patients with acute gastrointestinal anisakiasis].	using sds-page and immunoblotting assay, we analysed antigens defined by anti-anisakis larvae antibodies of ige and igg type in the sera of patients with acute gastrointestinal anisakiasis. 1) humoral ige response to 76, 64 and 60 kda molecular weight proteins of anisakis larvae and igg response to 91, 76, 64 and 60 kda molecular weight proteins were demonstrated in the sera of patients with acute gastric anisakiasis. those humoral antibody responses were also demonstrated in the sera of two pat ...	1990	2388423
capsaicin and histamine antagonist-sensitive mechanisms in the immediate allergic reaction of pig airways.	the airway vascular and bronchial responses were studied in pigs sensitized with ascaris suum. ascaris, histamine (h) and capsaicin aerosol all induced a clear-cut increase in blood flow in the nasal, laryngeal and bronchial circulation with a decrease in vascular resistance of 20-40%. when delivered to the lung both ascaris and histamine, but not capsaicin, caused pulmonary airflow obstruction with increase in resistance and a fall in dynamic compliance of 40-70%. after pretreatment of pigs wit ...	1990	2309568
inhibitory effect of renal nerve activity during canine anaphylactic hypotension.	we have examined baroreceptor regulation of renal nerve activity (rna) during anaphylactic hypotension that affects renal nerve activity. in anesthetized dogs, mean blood pressure (mbp), heart rate (hr), and rna were simultaneously measured. to test for a normally functioning baroreceptor reflex in each animal, a transient hypotension was induced by an intravenous injection of sodium nitroprusside (4 micrograms/kg). nitroprusside produced a reflex increase in rna (+63 +/- 12% at -20 mmhg and +13 ...	1990	2309932
in vitro induction of crawling in the amoeboid sperm of the nematode parasite, ascaris suum.	in a highly synchronous process, the immotile spermatids of ascaris suum extend pseudopods and become rapidly crawling sperm when treated with an extract from the glandular vas deferens of the male under strict anaerobic conditions. within 9-12 min, a pseudopod develops, elongates rapidly, and exhibits a continuous flow of membrane specializations, the villipodia, from tip toward base. when attached to acid-washed glass, the pseudopod pulls the cell body along at speeds exceeding 70 microns/min. ...	1990	2311127
structural studies on three flavin-interacting regions of the flavoprotein subunit of complex ii in ascaris suum mitochondria.	the flavoprotein (fp) subunit of mitochondrial complex ii contains covalently bound fad as a prosthetic group. in this study, the primary structure of the flavin-bound tryptic peptide from the fp subunit of ascaris complex ii was determined and found to be highly similar to those of the corresponding flavin-binding regions of bovine heart and bacterial fp subunits. furthermore, the ascaris fp subunit was shown to contain two regions exhibiting striking sequence similarity to the segments that ha ...	1990	2335235
n-terminal amino acid sequence identity between a major allergen of ascaris lumbricoides and ascaris suum, and mhc-restricted ige responses to it.	a protein allergen of the parasitic nematode ascaris has been purified to homogeneity by immunoaffinity chromatography. it is the most abundant protein species in the parasite's body fluid and has been named aba-1. the allergen's molecular weight (mw) has been previously estimated at 14,000, but this sizing is currently under re-evaluation. the immunological activity of the protein was intact after purification, as attested by immunoprecipitation and passive cutaneous anaphylaxis. the ige respon ...	1990	2335378
acute topical steroid administration blocks mast cell increase and the late asthmatic response of the canine peripheral airways.	previous studies indicated that mast cell number increased after airway exposure to ascaris suum antigen (ag). the following two series of experiments were performed to test the hypothesis that this phenomenon may be associated with the ag-induced late-phase bronchoconstriction (lar). in the first, two bronchoscopes wedged in airway segments of two contralateral lobes of 16 dogs were used to deliver 0.26, 2.6, or 26 micrograms of ag to one lobe; the other served as a control. after the observati ...	1990	2301857
biotin as a probe of the surface of ascaris suum developmental stages.	sulfo-nhs-biotin (aqueous soluble) and nhs-biotin (organic soluble) labeled similar sds-2me (sodium dodecyl sulfate/beta-mercaptoethanol) soluble cuticular proteins of second stage larvae (l2) and third stage ascaris suum larvae (l3). comparable analysis of biotin-labeled fourth stage larvae (l4), young adults, and mature adult ascaris suum revealed strong labeling of several sds-2me soluble cuticular proteins with nhs-biotin, while sulfo-nhs-biotin appeared to strongly label a single sds-2me so ...	1990	2385267
biophysical transport properties of the cuticle of ascaris suum.	the transport properties of isolated cuticle from ascaris suum were studied using standard two-chamber diffusion cells and a number of radiolabeled permeants which varied in molecular size, lipophilicity and electrical charge. the permeability coefficient of the collagen matrix (lipid-extracted cuticle) vs. molecular radius relationship showed the interdependence of molecular size and electrical charge of the permeants with respect to the aqueous pores of the negatively charged matrix. the perme ...	1990	2398915
penetrating the conjunctival barrier. the role of molecular weight.	dinitrophenyl (dnp) derivatives of various molecular weights were tested for their ability to elicit ocular anaphylaxis after topical application to the eye of immunized animals. adult male sprague-dawley rats were immunized by intraperitoneal injection of dnp-ascaris suum extracts and alum and were then skin-tested with dnp-bovine serum albumin on day 13 post-immunization to assess their sensitivity to the dnp hapten. on day 14, animals were challenged topically with dnp derivatives in one eye; ...	1990	2303328
acquired resistance to migrating larvae of ascaris suum in young pigs by repeated drug-abbreviated infections.	cross-bred 3- and 8-wk-old pigs were used to test whether drug-abbreviated infections with ascaris suum can stimulate acquired resistance to challenge. during the immunization period, both age groups of animals were infected with increasing numbers of a. suum eggs (500, 1,000, 2,000, 5,000, 10,000, and 20,000) at 7-day intervals while the pigs were receiving pyrantel tartrate in the feed. two days after the last infective dose, animals were placed on unmedicated feed for 8 days and then challeng ...	1990	2352069
[helminth infections in wild boars in enclosures in southern germany: species spectrum and infection frequency].	to determine the helminth fauna of wild boars, stomachs and intestines of 124, lungs of 62, and livers of 39 animals from 5 enclosures in southern germany were examined. the evaluation of the species composition revealed at least 13 species: metastrongylus apri, m. salmi, m. pudendotectus, physocephalus sexalatus, hyostrongylus rubidus, globocephalus longemucronatus, ascaris suum, trichuris suis, oesophagostomum dentatum, o. quadrispinulatum, fasciola hepatica, c. tenuicollis and capillaria. the ...	1990	2264058
molecular cloning and expression of an immunodominant 53-kda excretory-secretory antigen from trichinella spiralis muscle larvae.	a trichinella spiralis cdna expression library was constructed in lambda gt11 from muscle larvae mrna and immunologically screened to identify genes encoding previously described immunodiagnostic excretory-secretory (es) antigens. screening the library with t. spiralis infection serum from swine or rabbit antiserum to t. spiralis es antigen identified one clone, designated tsa-12, that contains a cdna transcript 539 bp in length and codes for an apparent 123-kda beta-galactosidase fusion protein ...	1990	2270099
the specificity of the antibody response to internal antigens of ascaris: heterogeneity in infected humans, and mhc (h-2) control of the repertoire in mice.	children from an area of africa endemic for the large roundworm of humans, ascaris lumbricoides, were found to vary considerably in the specificity of their serum igg response to the internal antigens of the parasite. this was particularly noticeable for responses to a 14-kd protein (aba-1) of the parasite that has previously been shown to be the subject of a strong ige antibody response in infected animals. the possibility that this heterogeneity in immune repertoire has a genetic basis was exp ...	1990	2357848
effect of nonadrenergic noncholinergic inhibitory nerve stimulation on the allergic reaction in cat airways.	to examine the effect of nonadrenergic noncholinergic (nanc) inhibitory nerve stimulation on the antigen inhalation with allergic animals, changes in pulmonary resistance (rl) and arterial plasma histamine concentration ([h]) caused by inhalation of ascaris suum antigen were studied in five control (group a) and five nerve-stimulated (group b) cats, which were anesthetized and mechanically ventilated. all animals were actively sensitized with ascaris antigen before the experiment. after choliner ...	1990	2297183
tumor necrosis factor-like cytotoxicity and anorexia in ascaris suum infected pigs.	tumor necrosis factor-like cytotoxicity (tnf-lc) was examined in sera from 12 pigs experimentally infected with ascaris suum. the difference of tnf-lc levels between eight infected and four uninfected controls was not significant. when an endotoxin challenge was intravenously administered 1 month after the first dose of a. suum, the levels of tnf-lc in the sera of infected pigs were one-third that of the controls 125 min post-challenge (pc). in a more detailed study on four infected and two unin ...	1990	2382388
[trypsin and alpha-amylase activities in the pancreas of guinea pigs. iii. effect of homogenates of invasive ova of ascaris suum and organic acids c2-c6 on the activity of enzymes in pancreatic extracts].	the studies were carried out on pancreas extracts from guinea pigs free of parasite invasion. the activity of trypsin was determined according to the method of anson, and amylase of fennel method. the measurements of activity were done at the presence of homogenized invasive eggs of ascaris suum, and the mixture of volatile organic acids c2-c6 (at concentrations of 30.3 and 0.3 mm/l). in samples with homogenized invasive eggs of a. suum the amylase activity remained unchanged while the activity ...	1990	2256339
evidence for the frequent use of ttg as the translation initiation codon of mitochondrial protein genes in the nematodes, ascaris suum and caenorhabditis elegans.	data obtained from alignments of nucleotide sequences of mitochondrial (mt) dna molecules of the nematode worms ascaris suum and caenorhabditis elegans indicate that in six of the mt-protein genes of a. suum and three of the mt-protein genes of c. elegans ttg is used as the translation initiation codon. also, gtt seems to be the translation initiation codon of the a. suum coiii gene. all of the five remaining a. suum mt-protein genes appear to begin with att and the remaining nine c. elegans mt- ...	1990	2235493