| p-1 blood group substance in lumbricus terrestris (earthworm) and ascaris suum. | | 1966 | 5956327 |
| vaccination against ascaris suum, ascaris lumbricoides and trichinella spiralis. i. peroral vaccination against ascaris suum, ascaris lumbricoides and trichinella spiralis with internal organs and musculature, infested with the larvae of the corresponding species. | | 1966 | 5987633 |
| [the effect of naphthamone (alcopar) and its derivatives of glucose consumption and production of volatile fatty acids in ascaris suum (goeze, 1782)]. | | 1966 | 6003155 |
| increased resistance in mice to larval ascaris suum infection induced by nippostrongylus brasiliensis. | | 1967 | 6017232 |
| [effect of sterinol on the survival of ascaris suum eggs in vitro]. | | 1967 | 6068778 |
| poly(adp-ribosylation) in ascaris suum. | poly(adp-ribosylation) was demonstrated in the intestinal parasite ascaris suum, especially in the reproductive tissues. the activity of the adp-ribosyltransferase was found to depend on divalent cations and to be stimulated by deoxyribonuclease i about 5-fold. the reaction rate was optimal at a temperature of 30 degrees c and at ph about 8.4. the apparent km value for nad was estimated to be 0.2mm. the enzyme activity was effectively inhibited by nicotinamide (ki = 65 microm) benzamide (6 micro ... | 1984 | 6090302 |
| [effect of larvae of ascaris suum goeze on phosphatase activity of the guinea pig intestine]. | | 1984 | 6095540 |
| the effects of avermectin and drugs related to acetylcholine and 4-aminobutyric acid on neurotransmission in ascaris suum. | we have examined some aspects of the neuropharmacology of the nematode ascaris suum using a divided chamber and selective stimulation technique to localize the sites of action of drugs. these techniques enabled us to investigate separately excitatory neuromuscular transmission, inhibitory neuromuscular transmission, and transmission from interneurons to excitatory motorneurons. we find that a curare-sensitive mechanism is involved in the excitation of the excitatory motorneuron via interneurons. ... | 1984 | 6096713 |
| functional depression of h2 histamine receptors in sheep with experimental allergic asthma. | we tested the hypothesis that airway hyperresponsiveness to histamine in the allergic sheep is related to functional depression of h2 histamine receptors. thirteen of 32 sheep responded with bronchoconstriction to inhaled ascaris suum antigen (allergic sheep), and the remainder served as controls (nonallergic sheep). in the allergic sheep, 50 and 100 breaths of 5% histamine solution increased mean pulmonary resistance (rl) to 235% and 438% of baselines, respectively. the corresponding values in ... | 1983 | 6136540 |
| effects of 8-(2-fluoroethyl)-3 alpha-hydroxy-1 alpha h, 5 alpha h-tropaniumbromide benzilate (ba598br) on allergy- and drug-induced asthmas. | the effects of ba598br, a new atropine derivative possessing a quarternary ammonium salt structure, on the canine airway, including its antiasthmatic effects, were investigated. the in vivo airway resistance was determined using the modified konzett-rössler method. inhalation of 0.01% of ba598br had an inhibitory effect against acetyl-choline (ach, 10 micrograms/kg i.v.)-evoked bronchoconstriction. the effect of ba598br was more powerful and longer lasting than that of the same dose of atropine. ... | 1983 | 6139497 |
| ultrastructural observations on the cell surface of the intestinal epithelium of the nematode, ascaris suum. nature of the electronegative charge. | the intestinal epithelium of ascaris suum consists of a single layer of tall columnar epithelial cells that rest on a thick basal membrane in contact with the pseudocoelomic cavity. experiments were conducted on glutaraldehyde-fixed tissue to ascertain the nature of the electronegative charges associated with both the apical microvillar surface and basal membrane. a strong electronegative charge was demonstrated on the microvillar surface and basal membrane with ruthenium red and cationic ferrit ... | 1980 | 6153929 |
| respiratory hypersensitivity to ascaris extract in guinea pigs sensitized by aerosol. | guinea pigs exposed via the respiratory route to ascaris suum extract in aerosol form became hypersensitive. the hypersensitivity was detectable by inhalational bronchial provocation testing using a noninvasive plethysmography, by chopped lung histamine release, and by passive cutaneous anaplylaxis. the antibody responsible for the hypersensitivity seems to be directly associated with pulmonary tissue. a locally produced cytotropic pulmonary antibody, probably igg or ige may be causal. histopath ... | 1980 | 6159330 |
| eosinophilia in athymic nude (rnu/rnu) rats--thymus-independent eosinophilia? | we have investigated the ability of genetically athymic (nude) rats to develop an eosinophilia to stimuli which are known to cause an eosinophilia in normal rats. nude rats developed eosinophilia following (a) the pulmonary embolization of bovine gammaglobulin (bcg)-coated latex particles, and (b) experimental infection with the nematode parasite ascaris suum. eosinophilia in nude rats was not dependent on the presence of specific homocytotropic antibodies, haemagglutinating antibodies or allerg ... | 1981 | 6166546 |
| effect of intravenous histamine, allergen (ascaris suum extract) and compound 48/80 and inhaled allergen-aerosol on bronchoconstriction and histamine release. | the effect of a histamine releaser (48/80), an antigen (ascaris suum extract) and of exogenous histamine infusion on antigen aerosol-induced bronchoconstriction was investigated in 24 mongrel dogs. during these substance infusions, the high values of arterial plasma histamine did not correlate with the degree of bronchoconstriction. further, a severe bronchoconstriction with a relatively low arterial plasma histamine concentration was observed during antigen inhalation. during substance inhalati ... | 1981 | 6173907 |
| antigenicity of beta-lactam antibiotic preparations: production of ige antibodies to beta-lactam antibiotic and their cross-reaction within the antibiotic group. | balb/c mice were immunized with conjugate of benzylpenicillin or ampicillin with ascaris suum extract. the mice developed ige antibodies to penicillin, which were found to react with commercially available penicillin preparations in the pca system. elimination of polymerized penicillin by sephadex chromatography from tested preparations could not diminish their activities to elicit pca. high pressure liquid chromatographic (hplc) separation of pcg preparation yielded fractions unable to elicit p ... | 1982 | 6176550 |
| effect of beta-adrenergic stimulation on experimental canine anaphylaxis in vivo. | we studied beta-adrenergic modulation of antigen-induced release of histamine and changes in lung function in natively allergic, anesthetized dogs. after thoracotomy and bilateral cervical vagotomy, samples of peripheral lung frozen in situ were obtained for measurement of adenosine cyclic 3',5'-monophosphate (camp) and histamine. in 11 dogs with beta-adrenergic blockade (propranolol), subsequent ascaris suum antigen (intravenous) decreased lung tissue histamine 21.5% +/- 5.3% (mean +/- se), wit ... | 1983 | 6185556 |
| induction of ige-mediated cutaneous, cellular, and airway reactivity in rhesus monkeys by ascaris suum infection. | in order to maintain a colony of rhesus monkeys with ige-mediated ascaris asthma, we evaluated the response of four animals to infection with ascaris suum ova. all four animals developed immediate-type cutaneous reactivity and airway responses. serum ige and igg antibody responses against ascaris antigen, as quantitated by the elisa, were seen in all animals. peripheral blood histamine release after stimulation with ascaris antigen was positive in all animals. airway reactivity to aerosol challe ... | 1983 | 6189928 |
| [attempt to isolate from ascaris suum an antigenic fraction for use in the immunologic diagnosis of onchocerciasis]. | for the purpose of immunological diagnosis of onchocerciasis, the authors attempted to isolate an antigenic fraction from ascaris suum which would only detect anti-onchocerca volvulus antibodies. chromatofocusing was done on a crude antigenic preparation from the ascaris. immunoelectrophoresis showed that the fraction eluted between ph 5.6 and ph 4.9 contained antigens which reveal anti-onchocerca antibodies; anti-loa loa antibodies are revealed by the fraction eluted at ph less than 4. from the ... | 1983 | 6192880 |
| the effects of nifedipine and verapamil on antigen-induced bronchoconstriction in dogs. | the effects of calcium channel blockers, nifedipine and verapamil (i.v. and aerosol), were investigated in beagle dogs natively allergic to ascaris suum antigen. control exposures to an aerosol of ascaris antigen provoked significant bronchopulmonary changes, i.e., increases in pulmonary resistance (rl) and decreases in dynamic lung compliance (cdyn). pretreatment with either nifedipine or verapamil (200 micrograms/kg, i.v.) provided significant inhibition in the rl responses to ascaris antigen ... | 1983 | 6194999 |
| laboratory infection of primates with ascaris suum to provide a model of allergic bronchoconstriction. | wild-caught non-human primates are naturally sensitive to ascaris antigen and provide a useful model for studying atopic asthma. the present study was carried out to determine the effect of experimentally infecting home-bred macaques with the nematode ascaris suum and hence provide an alternative for the naturally occurring model. following oral infection with the parasite the animals developed a blood eosinophilia and specific antibodies to purified ascaris antigen. these antibodies appeared to ... | 1983 | 6197218 |
| activities of some enzymes in the perienteric fluid of ascaris suum. | activities of 12 enzymes (amylase, lipase, cholinesterase, nonspecific carboxyl esterase, lactate dehydrogenase (ldh), alkaline phosphatase, glutamate-oxalacetate transaminase (got), glutamate-pyruvate transaminase (gpt), gamma-glutamyl transferase (gamma-gt), leucine aminopeptidase (lap), malate dehydrogenase (mdh) and peroxidase) were determined in the perienteric fluid and homogenate of ascaris suum. with the exception of amylase, all activities were higher in the homogenate than in the perie ... | 1983 | 6198263 |
| ascaris suum: continuous perfusion of the pseudocoelom and nutrient absorption. | a double-cannulation apparatus was constructed for continuous perfusion of the pseudocoelom of adult ascaris suum while maintaining the intact parasite in a controlled incubation chamber. peristaltic pumps maintained a constant flow rate of artificial perienteric fluid through the incubation chamber (1 ml/min) and through the parasite (100 microliters/min). based on protein determinations, perienteric fluid was removed from the pseudocoelom within 35 min of initiation of perfusion (3.5 ml). a no ... | 1984 | 6201385 |
| indication of a certain molecular correlation between rat ige and human ige. | the pca-activity of rat sera sensitized with ascaris suum antigen can be absorbed by antihuman ige and by rabbit or sheep antihuman ige convalently bound to sephadex particles or paper discs respectively. in vitro, rat immune sera show positive results using the human rist-system abolished by preheating for 2 hours at 56 degrees c and therefore excluding an interaction of igg 4. in contrast, no rat sera activity was seen using the human prist-system. these results agree with the concept of a sin ... | 1984 | 6205574 |
| the role of slow-reacting substance of anaphylaxis in the late bronchial response after antigen challenge in allergic sheep. | allergic sheep respond to inhalation challenge with ascaris suum antigen with an immediate bronchoconstriction that resolves within 2 to 4 h, followed by a second bronchial obstruction occurring 6.5 to 8 h after challenge. the purpose of this study was to determine if the antigen-induced late bronchial response in allergic sheep is mediator dependent and to determine the extent to which histamine and/or slow-reacting substance of anaphylaxis (srs-a) are involved in this response. to do this, 10 ... | 1984 | 6208832 |
| late phase bronchial obstruction following nonimmunologic mast cell degranulation. | immunologic degranulation of airway mast cells after antigen inhalation produces early and late airway obstructions in allergic sheep. in this study we determined whether nonimmunologic degranulation of airway mast cells by inhalation of compound 48/80 had similar effects. in five sheep, pulmonary flow resistance (rl), thoracic gas volume (vtg), and arterial o2 tension (pao2) were determined prior to and at predetermined times after inhalation of 48/80 aerosol. immediately after challenge mean s ... | 1984 | 6209256 |
| fenbendazole as a therapy for naturally acquired stephanurus dentatus and gastrointestinal nematodes in feral swine. | adult feral swine, naturally infected with kidney worms (stephanurus dentatus) and gastrointestinal nematodes, were divided into two groups of 10 pigs each. one group was treated with fenbendazole (panacur, hoechst ag, frankfurt am. main) mixed in feed at the rate of 3 mg kg-1 body weight for 3 days. the second group received feed only and was designated as non-treated controls. the animals in both groups were necropsied 3 weeks post-treatment and examined for the presence of live and dead adult ... | 1981 | 6211818 |
| mitochondrial ultrastructural and atpase changes during the life cycle of ascaris suum. | | 1982 | 6216389 |
| immune responses of swine to oral inoculation with embryonated eggs of ascaris suum. | responses of swine to oral inoculation with embryonated eggs of ascaris suum were monitored, using lymphocyte blastogenesis assays, indirect radioimmunoassays, and peripheral eosinophil counts (ec). transient cell-mediated immune responses of peripheral lymphocytes were detected by lymphocyte blastogenesis assay as early as postinoculation day (pid) 2, but were rarely positive for consecutive samples taken at 2-day intervals. humoral antibodies were first detected at pid 6 to 17 by indirect radi ... | 1982 | 6216835 |
| phosphofructokinase from ascaris suum muscle. | | 1982 | 6218373 |
| clinical pathology of experimental stephanuriasis in pigs. | haematology and serum aspartate aminotransferase and alanine aminotransferase activities were studied during the migration phase of stephanurus dentatus in the livers of experimentally infected pigs. there was no evidence of anaemia but total leucocyte counts were raised and peripheral eosinophilia began 2 to 3 weeks after infection. peak eosinophilia occurred 6 to 7 weeks after infection and levels were still elevated at 20 weeks. lymphocyte and neutrophil numbers remained constant. reinfection ... | 1983 | 6226277 |
| preparation and characterization of a monoclonal anti-t helper factor antibody. | sprague-dawley rats were immunized by injection of antigen b-specific t helper factor thf) eluted from sepharose-antigen d adsorbents. rat spleen cells from animals immunized with thf were fused with a balb/c tumour cell (p3x63-ag8.653) to prepare monoclonal anti-thf antibodies. the hybrids produced were screened for anti-thf antibodies by an enzyme-linked immunoassay (elisa), and we shall describe the characteristics of one of the hybrid (hybridoma 6-2.2 anti-thf) antibodies produced. (i) the m ... | 1984 | 6231242 |
| [the place of attack of beta-2 receptor stimulating bronchodilators. fenoterol (berotec) in allergic airway obstruction in dogs, hypersensitive to ascaris suum]. | on dogs sensitive against ascaris suum extract an increase of the airway resistance can be caused by challenge alone on the upper part or the lower part of the airways by ascaris suum extract. fenoterol as beta 2-receptor stimulator is capable to decrease this reaction by deposition of this drug on the upper part as on the lower part of the airways. the effect of fenoterol on the upper part of the airways is much stronger and lasts much longer than on the lower part of the airways or by intraven ... | 1980 | 6248681 |
| surface receptors: are they involved in transformation of spermatozoa of ascaris? | exposure of the spheroidal spermatozoa of ascaris suum to an extract of the male accessory gland causes their transformation into ameboid cells. we have investigated the mechanism of this transformation, also termed activation, by labeling the proteins of accessory gland extracts with fluorescein isothiocyanate (fitc) or [125i], followed by qualitative localization of the sperm activating substances (sas) and quantitative measurements of [125i]-sas binding. fluorescent patches of fitc-conjugated ... | 1981 | 6260362 |
| [survival of ascaris eggs, salmonella and fecal coli in soil and on vegetables grown in infected soil (author's transl)]. | in an experiment it was shown that eggs of ascaris suum, salmonella and fecal coliform bacteria mixed with sewage sludge and added to soil, will survive the first growing season. most eggs of ascaris suum in soil embryonated within a period of 7--15 weeks. in soil without a protective vegetation the concentration of eggs in the top soil layer 0--3 cm decreased faster than in soil with a protective vegetation. at the same time there seemed to be a corresponding increase in the concentration of eg ... | 1981 | 6261218 |
| restriction enzyme analysis of the germ line limited dna of ascaris suum. | the germ line limited dna of ascaris suum was isolated from sperm and testis as a satellite dna component in hoechst 33 258 -- cscl gradients. employing restriction enzyme analysis, we show that the germ line limited dna is composed entirely of two families of tandemly repeated sequences, one repeat unit is 125 bp, and the other 131 bp long. the total appr. 5 x 10(5) copies of the two families are physically separated from each other (segmental arrangement). several repeat unit variants within b ... | 1981 | 6268372 |
| serotonin (5-hydroxytryptamine): a possible regulator of glycogenolysis in perfused muscle segments of ascaris suum. | | 1981 | 6269541 |
| effects of nitric acid on carbachol reactivity of the airways in normal and allergic sheep. | the airway effect of a 4-hr exposure (via a plexiglas hood) to 1.6 ppm nitric acid vapor were evaluated in seven normal and seven allergic sheep, i.e., animals that have a history of reacting with bronchospasm to inhalation challenge with ascaris suum antigen. the nitric acid vapor was generated by ultrasonic nebulization of a 2% nitric acid solution. airway effects were assessed by measuring the change in specific pulmonary flow resistance before and after a standard inhalation challenge with 2 ... | 1982 | 6277256 |
| correlation of muscle activity with glycogen metabolism in muscle of ascaris suum. | isolated muscle segments from the parasitic roundworm ascaris suum were shown to contract when perfused with acetylcholine (ach). the muscle responded to ach concentrations of 1 microm and was maximally contracted at 50 microm ach. in fed muscle segments perfused with saturating levels of ach the glycogen synthase ka values for glucose 6-phosphate increased from 0.5 to 0.95 mm. in starved segments stimulated by ach, the muscle utilized glycogen at a rate that was 1.41 micrograms.min-1.g tissue-1 ... | 1982 | 6282145 |
| chromatin diminution in ascaris suum: nucleotide sequence of the eliminated satellite dna. | we have determined the prototype sequence of the dna which is eliminated in the course of chromatin diminution in ascaris suum. this dna which is virtually absent from somatic cells but retained in the germ line consists predominantly of highly repetitive sequences which are variants of an at rich 123 base pair repeat unit. both major and minor variants have been sequenced. the overall structure of this germ line limited dna corresponds to the segmental organization characteristic of satellite d ... | 1982 | 6285304 |
| the role of cyclic amp-mediated regulation of glycogen metabolism in levamisole-perfused ascaris suum muscle. | the effects of levamisole on muscle contraction and glycogen metabolism have been examined in isolated muscle-cuticle sections of the roundworm ascaris suum. muscle contraction occurred when various levels of levamisole were perfused through the preparation. at a levamisole concentration of 0.42 mm, the period of contraction lasted only about 6 min and was followed by a period of relaxation. during this relaxation period, there was an activation of glycogen synthase (ec 2.4.1.11), as evidenced b ... | 1983 | 6300647 |
| plasmodium berghei: eosinophilic depression of infection in mice. | the effects of eosinophilia on the course of plasmodium berghei infection in mice were studied. eosinophilia was induced by intravenous injection of ascaris suum body fluid into the mice. results indicated that eosinophils may play a role in the suppression of murine malaria. a significant reduction in parasitemias and increased survival time in eosinophilic mice occurred compared to mice not treated with a. suum body fluid. reduction of parasitemia was effectively achieved when the mice were ch ... | 1984 | 6363116 |
| inhibition of antigen-induced bronchoconstriction by methylprednisolone succinate. | we investigated whether the glucocorticoid methylprednisolone succinate mg/kg) could prevent antigen-induced bronchoconstriction in conscious sheep. ten allergic ewes were subjected to inhalation challenge with ascaris suum antigen, with and without methylprednisolone pretreatment, administered intravenously either 20 min or 3 hr before antigen challenge. antigen challenge alone resulted in increased airflow resistance, pulmonary hyperinflation, and decreases in dynamic lung compliance and arter ... | 1984 | 6368648 |
| airway responses in offspring of dogs with and without airway hyperreactivity. | we examined airway responsiveness to aerosols of ascaris suum, citric acid, and methacholine chloride in the offspring of two highly allergic breeds of dogs: basenji-greyhounds (bg) and basenjis (b). the bg parents had airway hyperresponsiveness to citric acid and methacholine, whereas the b parents did not. both bg and b offspring were allergic as measured by many positive skin tests. bg offspring, like their parents, were hyperresponsive to citric acid and methacholine, whereas b offspring wer ... | 1984 | 6373693 |
| field studies with in-feed medication of pigs in the netherlands using the anthelmintic thiophanate with particular reference to efficacy against ascaris suum. | studies on the efficacy in pigs of low level in-feed medication with the anthelmintic thiophanate at a minimum intake of 6 mg/kg/day for fourteen days are reported. a trial was conducted to compare a group of medicated fattening pigs with a similar unmedicated group on premises known to have a high challenge of ascaris spp. daily growth rate was improved whilst feed conversion ratio and the liver condemnation rate were reduced in the treatment group. routine medication of a whole herd using this ... | 1984 | 6375107 |
| immunomodulation by nematodes: a review. | there is current evidence that infections with trichinella spiralis, ascaris suum, nippostrongylus brasiliensis, nematospiroides dubius (syn. heligmosomoides polygyrus) and diverse filariae affect the immune responsiveness of their hosts. t. spiralis, or its extracts, can depress or enhance the heterologous humoral or cell-mediated immunities, and affect macrophage activity or the response to other invaders. these effects are induced by products of the migratory and early muscle larvae and appea ... | 1984 | 6382784 |
| purification and characterization of excretory and secretory antigen of toxocara canis larvae. | excretory/secretory (es) antigen of toxocara canis larvae was isolated from the culture medium of second-stage larvae by gel filtration. the antigen induced production of not only igm or igg but also ige antibody. the antigen, when treated with guanidine hydrochloride and 2-mercaptoethanol, lost its allergenic activity but retained an ability to induce igm or igg antibody. purified antigen was of glycoprotein nature and had a molecular weight of 35,000. the whole antigen showed a cross-reaction ... | 1983 | 6411609 |
| pulmonary uptake of mannitol as an index of changes in lung epithelial permeability. | to develop a simple, inexpensive, and nontoxic test for changes in bronchial permeability we have employed a specific gas chromatographic assay to measure the uptake of mannitol from the lung after administration of an intratracheal aqueous bolus (8 ml of 5% solution). we have tested the ability of our method to detect the known increase in lung epithelial permeability elicited by challenge of previously sensitized animals with aerosols of ascaris suum antigen. in ascaris-sensitive basenji-greyh ... | 1983 | 6413466 |
| purification and characterization of phosphorylase b from ascaris suum. | glycogen phosphorylase b (ec 2.4.1.1) has been purified from the muscle of the roundworm, ascaris suum. the 223-fold purified enzyme was shown to be homogenous by high performance liquid chromatography (hplc), gel filtration column chromatography and sodium dodecyl sulfate (sds) gel electrophoresis. the apparent native molecular weight of the enzyme determined by size exclusion chromatography by hplc and gel filtration corresponded to 200 000 and 199 000, respectively. the subunit molecular weig ... | 1983 | 6419099 |
| [dynamics and distribution of t- and b-dependent lymphocytes in the lymphoid organs of guinea pigs in experimental ascariasis]. | after experimental invasion of guinea-pigs with the eggs of ascaris suum (5,000 eggs per animal), the dynamics and distribution of t and b-dependent cells in lymphoid organs (spleen, mesenteric, hepatic and mediastinal nodes) were studied. the detection of both cell populations by means of rosette methods (e, ea, eac) showed that in all studied organs their first increase took place on the first and second day after invasion. on the fifth to seventh day after invasion the maximum increase in t c ... | 1984 | 6420974 |
| potentiation of an ige-like response to bordetella bronchiseptica in pigs following ascaris suum infection. | ascaris suum-infected and uninfected pigs were vaccinated with bordetella bronchiseptica bacterin at 8 and 28 days of age. only the infected pigs gave type i cutaneous hypersensitivity to the bacterin when tested at 80 days of age. only the native sera of infected pigs caused passive cutaneous anaphylaxis 24 h after inoculation into guinea pigs. heating the sera at 56 degrees c for 4 h abolished this property. mice infected with a. suum for 34 days did not react to the bacterin. an a. suum infec ... | 1984 | 6440350 |
| phosphofructokinase from ascaris suum. purification and properties. | a rapid and efficient procedure has been developed to purify phosphofructokinase from the muscle of the parasitic roundworm, ascaris suum. the procedure can be accomplished in 1 day with a 420-fold purification and a 60% yield. the enzyme was shown to be homogeneous by two-dimensional electrophoresis, sepharose 6b column chromatography, and high performance liquid chromatography utilizing a size exclusion column. the subunit molecular weight of the enzyme was found to be 95,000 by electrophoresi ... | 1982 | 6460770 |
| phosphofructokinase from ascaris suum. regulatory kinetic studies and activity near physiological conditions. | | 1982 | 6460771 |
| phosphofructokinase from ascaris suum. the effect of phosphorylation on activity near-physiological conditions. | | 1982 | 6460772 |
| [bronchoconstrictor reaction pattern of the upper respiratory tract and peripheral bronchial system]. | ascaris suum hypersensitive dogs were exposed to ascaris aerosol, histamine, and acetylcholine at different time intervals separately to the upper part of the airtract and to the lower airways. before and during the exposure the plasma histamine concentrations were measured. acetylcholine and histamine delivered at the upper part of the airtract do not cause bronchoconstriction. histamine diffuses in relatively high amounts through the mucosa of the upper part of the airtract into the arterial p ... | 1984 | 6463389 |
| amino acid and lipid composition of refringent granules from the ameboid sperm of ascaris suum (nematoda). | transformation of the spermatozoon of ascaris suum from a spheroidal to an ameboid cell is associated with the formation of a motile pseudopodium and coalescence of the intracellular refringent granules. the pseudopodia of the ameboid spermatozoa contain filaments organized into dense patches, bundles, web-like or lace-like networks, as observed by electron microscopy. the morphology and chemistry of the refringent granules were investigated in subcellular fractions enriched for these structures ... | 1984 | 6469722 |
| the binding and subsequent inhibition of tubulin polymerization in ascaris suum (in vitro) by benzimidazole anthelmintics. | benzimidazole anthelmintics may act by interfering with the microtubule system in ascaris suum. binding of benzimidazole anthelmintics, and their inactive metabolites, to a. suum tubulin was demonstrated by the inhibition of intestinal extracts of the nematode to bind [3h]colchicine. in addition, these compounds inhibited the polymerization of tubulin into microtubules in vitro. | 1984 | 6487354 |
| parasitic liver damage in lambs with particular reference to the migrating larvae of ascaris suum. | it has been suggested that the larvae of the pig nematode ascaris suum is a possible cause of rejection of lambs' livers in the abattoir. however, this assertion was not substantiated in an experimental study in which lambs were dosed with infective a suum eggs. this produced, at the worst, small trivial lesions (up to 2 mm diameter) which would probably have been missed at meat inspection under commercial conditions. no evidence of ascarid lesions was observed in the livers of 5873 lambs and ho ... | 1984 | 6495588 |
| an ultraviolet-attenuated egg vaccine for swine ascariasis: parameters affecting the development of protective immunity. | pigs inoculated with uv-irradiated ascaris suum eggs developed immunity against challenge exposure with a suum infective eggs. trials were conducted to determine the optimal conditions necessary to induce high levels of protective immunity and minimize liver lesions related to larval migration. protective immunity was defined as the percentage of reduction in the recovery of larvae from the lungs of inoculated pigs, compared with controls, 7 days after challenge exposure with 5 x 10(3) or 10(4) ... | 1984 | 6497109 |
| vernal conjunctivitis. model studies in guinea pigs immunized topically with fluoresceinyl ovalbumin. | repeated topical applications of fluoresceinyl ovalbumin (fl-oa) to the conjunctival sac of guinea pigs sensitized them for conjunctival type 1 hypersensitivity reactions and mast cell degranulation. guinea pigs infected with ascaris suum, with high titers of circulating anti-a suum ige and igg1 antibody, also produced conjunctival type 1 reactions on topical challenge with a suum antigen. these reactions were no more intense than those of animals topically sensitized and challenged with fl-oa, ... | 1984 | 6497752 |
| [long-term effects of a deworming program using flubendazole and levamisole on the percentage of condemned livers in slaughtering pigs]. | a deworming programme continued over a period of twenty-four months was carried out to study the proportion of livers condemned because of milk spots, which was unacceptably high on a pig-fattening farm in the province of limburg, the netherlands. in order to remove ascaris suum from the fattening pigs initially present and to prevent subsequent contamination of the houses with worm eggs, three courses of deworming treatment with flubendazole were initiated on the farm. all newly incoming piglet ... | 1984 | 6506051 |
| kinetic mechanism in the direction of oxidative decarboxylation for nad-malic enzyme from ascaris suum. | measurement of the initial rate of the malic enzyme reaction varying the concentration of nad at several different fixed levels of mg2+ (0.25-1.0 mm) and a single malate concentration gave a pattern which intersects to the left of the ordinate. repetition of this initial velocity pattern at several additional malate concentrations and treatment in terms of a terreactant mechanism suggests an ordered mechanism in which nad adds prior to mg2+ which must add prior to malate. on the other hand, when ... | 1984 | 6509028 |
| determination of dissociation constants for enzyme-reactant complexes for nad-malic enzyme by modulation of the thiol inactivation rate. | incubation of nad-malic enzyme from ascaris suum with the sulfhydryl reagents n-ethylmaleimide (nem), 5,5'-dithiobis(2-nitrobenzoic acid) (dtnb), or 4,4'-dithiodipyridine (4-pds) results in rapid and complete loss of malate oxidative decarboxylase and pyruvate reductive carboxylase activities. with dtnb, this loss of activity occurs concomitantly with the modification of about 1 thiol group per subunit. the majority of the activity is lost when 0.5 thiol per subunit is modified, indicative of po ... | 1984 | 6509029 |
| immune response in rabbits experimentally infected with ascaris suum. | immune response of rabbits experimentally infected with ascaris suum was studied by indirect haemagglutination method. the animals were infected with the doses of 5,000, 10,000 and 20,000 infective eggs per animal. positive reactions were observed from days 5-11 p.i., maximum reactions on days 11-19 p.i. a reinfection with the same doses (1x or 2x after 35 and 65 days) increased the antibody titre. the strongest individual reaction was recorded on day 19 p.i. in the group infected with the highe ... | 1984 | 6510836 |
| performance and tissue copper concentrations of control and ascaris suum-infected pigs fed excess dietary copper. | ninety-eight crossbred growing-finishing swine were used in 2 experiments to investigate the interaction between naturally occurring ascaris suum infections and excess copper ingestion. in both experiments, pigs were fed a 14% crude protein basal diet or the basal diet + 250 ppm copper. these 2 dietary groups were subsequently divided into 2 additional treatment groups (a. suum-infected or uninfected) based on worm recovery from the intestine or from fecal egg counts. excess dietary copper impro ... | 1984 | 6512632 |
| electron transfer complexes of ascaris suum muscle mitochondria: i. characterization of nadh-cytochrome c reductase (complex i-iii), with special reference to cytochrome localization. | an nadh-cytochrome c reductase (complex i-iii) was isolated from ascaris suum muscle mitochondria. the enzyme preparation catalyzed the reduction of 1.68 mumol cytochrome c min-1 mg-1 protein at 25 degrees c with nadh but not with nadph, and retained its sensitivity to rotenone, piericidin a and 2-heptyl-4-hydroxyquinoline-n-oxide as with the submitochondrial particles. the isolated complex i-iii, essentially free of succinate-cytochrome c reductase and cytochrome c oxidase, consisted of fourtee ... | 1984 | 6513990 |
| the fumarate reductase system as a site of anthelmintic attack in ascaris suum. | various benzimidazole compounds have been shown to be highly effective as inhibitors (up to 50% reduction of activity) in vitro of the helminth-specific enzyme, fumarate reductase, of ascaris suum. anthelmintically active and inactive benzimidazoles were similarly effective as inhibitors of enzyme activity. albendazole-induced inhibition of fumarate reductase was not observed when the enzyme was preincubated with nadh. | 1984 | 6518278 |
| culture requirements of ascaris suum larvae using a stationary multi-well system: increased survival, development and growth with cholesterol. | ascaris suum third-stage larvae (l3) were recovered from infected rabbits and cultured in a stationary multi-well plate system. several different culture media including medium 199, nctc-135, minimum essential medium-eagle, dulbecco's modified eagle's medium, rpmi 1640, mccoy's 5a and neuman-tytell medium were tested to determine which was best for overall larval survival, development and growth. larvae developed from l3 to fourth-stage (l4) in all media tested, but larval survival and the yield ... | 1984 | 6538364 |
| antibody responses to toxocara canis using sera from parasite-infected mice and protection from toxocariasis by immunisation with es antigens. | evidence for serological cross-reactions between toxocara canis and related nematode parasites has been sought using sera from infected mice and the immunodiagnostic excretory/secretory (es) antigen of t. canis larvae. sera from mice experimentally infected with either t. canis, t. cati, t. pteropodis, toxascaris leonina or ascaris suum were tested for the presence of antibodies to t. canis es antigen by solid-phase radioimmunoassay (ria) or enzyme-linked immunosorbent assay (elisa) using anti-m ... | 1984 | 6543490 |
| comparison of counterimmunoelectrophoresis with indirect haemagglutination test in the detection of antibodies in rabbits experimentally infected with various species of ascarids. | counterimmunoelectrophoresis (ciep) and indirect haemagglutination (iha) were used for the detection of antibodies in 437 sera of rabbits experimentally infected with ascaris suum, toxocara canis and toxascaris leonina. ciep showed 44.0-52.3% positivity in a. suum, 25.5% in t. canis and 19.7-29.2% in t. leonina infection, whereas the positivity detected by iha was 59.8-90.2% in a. suum, 44.8% in t. canis and 59.6% in t. leonina infection. a comparison of the two tests reveals that ciep is a suit ... | 1984 | 6589203 |
| isolation and properties of alpha-amylase from perienteric fluid of ascaris suum. | the properties of alpha-amylase from perienteric fluid of ascaris suum were studied. the enzyme is strongly activated by chlorides at a simultaneous shift of ph optimum. it is fully activated already at 10(-3) m concentration of chlorides. a method of alpha-amylase isolation was modified with respect to these properties to give the purification degree 278 and yield of 11%. | 1984 | 6611287 |
| in vitro interaction of eosinophils from ascarid-infected eyes with ascaris suum and toxocara canis larvae. | the role of eosinophils in ocular ascarid infection is studied in an animal model. primary intravitreal infection of guinea-pig eyes with ascarid (toxocara canis, ascaris suum) second-stage (l2) larvae resulted in an anterior chamber exudate containing 98% or more eosinophils. anterior chamber aspirates were cultured in rpmi media 1640 with t. canis or a. suum l2 larvae at 37 degrees c for 1-96 hours. the in vitro interaction of eosinophils with l2 larvae was studied by light, and scanning and t ... | 1983 | 6618795 |
| duration of mucociliary dysfunction following antigen challenge. | antigen-induced bronchospasm is associated with an impairment of airway mucociliary clearance, but the duration of this defect has not been examined. in the present study, we determined the time course of mucociliary dysfunction following inhalation challenge with ascaris suum extract in conscious sheep with hypersensitivity to this antigen. tracheal mucous velocity (tmv) as an index of mucociliary function, pulmonary resistance (rl), and arterial oxygen tension (pao2) were measured prior to, im ... | 1983 | 6629909 |
| factors contributing to the in vitro development of ascaris suum from second-stage larvae to mature adults. | when a three-step roller culture system was used, second-stage larvae of ascaris suum, artificially hatched from eggs, developed in high numbers to the fourth stage, and a few to young and mature adults. the culture system consisted of (1) medium kw-2 supplemented with 10 mm l-cysteine for the first 4 days, and with 5 mm l-cysteine for the following 7 days; (2) followed by medium api-18 for 7 days; and (3) thereafter, by medium api-1 supplemented with hemin (bovine) at a concentration of 24 micr ... | 1983 | 6631626 |
| characterization of a late phase pulmonary response after antigen challenge in allergic sheep. | we determined if allergic sheep undergo a dual bronchoconstriction in response to inhalation of antigen and whether these responses could be modified by cromolyn or glucocorticosteroids. in 8 conscious sheep with ascaris suum hypersensitivity, mean pulmonary flow resistance (rl), dynamic lung compliance (cdyn), thoracic gas volume (vtg), and arterial oxygen tension (pao2) were determined prior to, immediately after, and from 2 through 8 h after and 24 h after inhalation challenge with ascaris su ... | 1983 | 6638671 |
| antigen stimulates glycoprotein secretion and alters ion fluxes in sheep trachea. | we studied the effects of in vitro challenge with specific antigen (ascaris suum antigen) on glycoprotein secretion and ion fluxes in tracheal tissues from allergic sheep. we mounted tissues in perspex chambers and measured secretion of 35s- and 3h-labeled glycoproteins and fluxes of cl- and na+. in tissues from allergic sheep, a. suum antigen (25 micrograms protein x ml-1) increased glycoprotein secretion. a. suum antigen initially reversed net cl- flux, causing net absorption of cl- and of na+ ... | 1983 | 6643194 |
| [effect of pesticides: cartap and monocrotophos on the invasiveness of ova of ascaris suum and on the pathomorphological changes in the lung and liver of cavia porcellus with experimental ascariasis]. | | 1983 | 6649636 |
| cytoadherence effects of serum on ascaris suum infective larvae. | dead and live ascaris suum infective larvae were found to adhere to monolayer cultures of mouse peritoneal macrophages following incubation with normal rabbit serum (nrs) or with rabbit anti-a, suum (raas). cell-larval binding was shown to be affected by the numbers of larvae added to cell monolayers and by incubation time. larval adherence to cell monolayers was dependent upon the concentration of nrs or raas in the incubation mixture. dead larvae were not bound by macrophages in as significant ... | 1983 | 6649985 |
| ascaris-induced bronchoconstriction in primates experimentally infected with ascaris suum ova. | bronchial provocation with ascaris allergen evoked bronchoconstriction in home-bred macaca arctoides monkeys sensitized by experimental infection with embryonated ascaris suum ova. inhalation of ascaris allergen by aerosol prior to infection produced no changes in lung function. in infected animals total lung resistance (rl) increased and dynamic lung compliance (cdyn) decreased following ascaris inhalation. the changes in lung function reached a peak, 2-5 min after allergen inhalation, lasted f ... | 1983 | 6652969 |
| does atropine block antigen-induced contraction in canine bronchial rings? a preliminary report. | isometric contractions recorded with a strain gauge, were elicited with cumulative doses of ascaris suum antigen on fresh canine bronchial rings from 8 dogs. fifteen rings from 2 spontaneously sensitized dogs reacted with contractions to the antigen, while 11 rings from other dogs did not react. seven sensitized rings were re-challenged after atropine, which significantly prevented the antigen from contracting these rings at a time when antigen contracted 3 non-atropinized, simultaneously tested ... | 1983 | 6653686 |
| the localization of fumarase and malic enzyme in muscle mitochondria of ascaris suum. | the distribution of fumarase and malic enzyme in ascaris suum muscle mitochondria was investigated by employing digitonin fractionation techniques. the ability of these procedures to resolve the various submitochondrial fractions (intracristal space, inner membrane, matrix and inner membrane-matrix particles) was verified by electron microscopy and the distribution of appropriate marker enzymes. from the data obtained, it is concluded that fumarase in ascaris muscle mitochondria is located solel ... | 1983 | 6656845 |
| experimental ocular toxocara canis and ascaris suum infection: in vivo and in vitro study. | | 1983 | 6659031 |
| hemodynamic mechanisms in anaphylaxis. | intravenous administration of ascaris suum antigen (1 mg) as well as histamine (5 mg) was associated with rapid and marked reductions in cardiac output (qt) and systemic arterial pressure (pa) in anesthetized mechanically ventilated dogs. when venous return was maintained constant by an external pump reservoir system, both antigen and histamine caused rapid reductions in the reservoir blood volume consistent with peripheral pooling. in addition, interruption of the splanchnic arterial inflow by ... | 1983 | 6661813 |
| response of bronchial smooth muscle to mast cell degranulation in situ. | we studied the response of bronchial smooth muscle to mast cell degranulation with ascaris suum antigen (aa) and compound 48/80 (48/80) in 26 mongrel dogs in situ. bronchial smooth muscle response was measured isometrically in situ from a segment of the right middle lobe bronchus; tracheal response was monitored isometrically as a control. after intra-arterial (ia) injection of aa into the bronchial circulation, bronchial contraction preceded tracheal contraction by 19.2 +/- 4.6 s (p less than 0 ... | 1983 | 6662771 |
| white spots of the liver in pigs experimentally infected with ascaris suum. | to make clear the relationship between ascaris suum infection and the appearance of white spot lesions on the surface of the liver in pigs, three groups of pigs were inoculated orally with embryonated a. suum eggs and observed clinicopathologically. group a of three pigs were inoculated 21 times with 100 eggs each of the nematode, group b of three pigs 4 times with 50,000 eggs each for 10 weeks, and group c of two pigs 2 times with 50,000 eggs each at a one-week interval. all the pigs were sacri ... | 1983 | 6680775 |
| pathological studies on white spots of the liver in fattening pigs. | hepatic lesions were found in fattening pigs derived from a farm where swine had been suffering from the multiple occurrence of white spots in the liver. they were examined at a slaughter-house for 18 months. the white spots were classified into three patterns on the basis of the macroscopic appearance; that is a compact, a mesh-worked, and a lymphonodular pattern. histologically, the following 3 kinds of lesions were seen: (1) eosinophilic interstitial hepatitis accompanied with intralobular ne ... | 1983 | 6680776 |
| the measurement of ascaris suum protein by radioimmunoassay in sera from patients with helminthiasis and with gastrointestinal diseases. | in this study, we have developed a reliable radioimmunoassay (ria) for ascaris suum protein (asp) and have measured the serum concentration of asp in control subjects and in patients with helminthiasis and a variety of gastrointestinal diseases. the cross-reactivities of asp antibody with other helminth worm antigens were also measured by ria. ascaris lumbricoides contained approximately the same amount of asp antigenic substance as did ascaris suum. toxocara canis had a high concentration of a ... | 1983 | 6682964 |
| nippostrongylus brasiliensis and ascaridia galli: mitochondrial respiration in free-living and parasitic stages. | aerobic respiratory pathways have been delineated and respiratory efficiency has been assessed in mitochondria isolated from embryonated eggs, infective larvae, and adult nippostrongylus brasiliensis and ascaridia galli. mitochondrial respiration in free-living stages of n. brasiliensis is mediated mainly by a mammalian-like antimycin a- and cyanide-sensitive pathway; specific respiratory activity is high and oxidative phosphorylation efficient. in mitochondria of adult n. brasiliensis, antimyci ... | 1983 | 6683662 |
| antigens in perienteric fluid of ascaris suum as detected through antibodies in pigs orally inoculated with fully embryonated eggs. | perienteric fluid (pf) of adult ascaris suum was fractionated by ammonium sulfate precipitation, gel filtration or deae--cellulose chromatography, and sucrose density gradient centrifugation. anti-sera (anti-ee) from pigs which were inoculated orally with fully embryonated eggs (ee) of a. suum were used in an indirect radioimmunoassay (iria) to determine which fractions of pf reacted positively in the analyses at the lowest protein concentration. these fractions were considered to contain more p ... | 1983 | 6684353 |
| anthelmintic activity of ivermectin in pigs naturally infected with ascaris and trichuris. | the anthelmintic efficacy of ivermectin administered by subcutaneous (sc) injection or orally via the drinking water was investigated in feeder pigs naturally infected with ascaris suum and trichuris suis. group 1 pigs (n = 5) were given drinking water as a placebo, group 2 pigs (n = 5) were given ivermectin by sc injection (300 micrograms/kg of body weight), and group 3 pigs (n = 5) were given ivermectin in water also at a dosage rate of 300 micrograms/kg of body weight. at necropsy 11 days lat ... | 1983 | 6688706 |
| some experimental approaches to the study of nutrition and parasitic infection. | two experimental models to examine the relationship between host nutrition and parasitic infection are considered. moniliformis dubius, an acanthocephalan parasite of the laboratory rat, is dependent on the type and amount of host dietary carbohydrate for growth and reproduction. starch, glucose, fructose, and mannose in host diets support parasite growth and development, but much lower dietary amounts of starch, fructose, and mannose are needed compared to free glucose. the pig-ascaris suum rel ... | 1984 | 6692943 |
| nadh-dependent tiglyl-coa reduction in disrupted mitochondria of ascaris suum. | the incubation of 2-methylcrotonyl-coa with either succinate or nadh and disrupted ascaris suum mitochondria results in substantial 2-methylbutyrate formation. both membrane-bound and soluble components are required and the nadh-dependent reduction is rotenone sensitive, suggesting the involvement of the electron-transport chain. rat liver mitochondria, incubated under similar conditions, did not catalyze 2-methylbutyrate formation. however, the substitution of a. suum mitochondrial membranes fo ... | 1984 | 6694671 |
| use of a rast technique with ascaris suum allergen to detect a. lumbricoides infestation. | | 1984 | 6701708 |
| 2-methylbutyryl coa dehydrogenase from mitochondria of ascaris suum and its relationship to nadh-dependent 2-methylcrotonyl coa reduction. | acyl coa dehydrogenase and electron-transfer flavoprotein have been isolated and partially purified from mitochondria of the anaerobic nematode, ascaris suum. dehydrogenase activity was greatest with 2-methylbutyryl coa and the relative substrate specificities of the ascarid dehydrogenase(s) differ greatly from their mammalian counterparts. it appears that the ascarid dehydrogenase functions physiologically as a reductase, catalyzing the final step in the synthesis of branched-chain fatty acids. ... | 1984 | 6704106 |
| changes in the somatic musculature of the 4th-stage larvae of ascaris suum goeze, 1782 (nematoda: ascaridoidea) developing in swine. | fourth-stage larvae of ascaris suum, recovered from piglets at 11-22 days post-infection (p.i.), were examined by light microscopy for changes in the somatic musculature. during this time the estimated total number of muscle cells in the body increases from approximately 600 to 21 000 cells. this non-eutelic development appears to occur by division of platymyarian muscle cells into coelomyarian cells, thereby increasing the number of muscle cells/quadrant from 5 to 85. the incompleteness of thes ... | 1984 | 6709390 |
| mucosal surface lesions in young protein-deficient pigs infected with ascaris suum (nematoda). | the morphology of the mucosal surface of samples from the small intestine of young protein-deficient pigs was examined by scanning electron microscopy. three pigs had been infected with ascaris suum for at least 58 days while 1 had remained uninfected; all pigs were housed and maintained under the same conditions, at the same time. the tissue samples collected at post mortem examination from pigs which contained a. suum showed varying degrees of villous atrophy and fusion. in addition, unusual s ... | 1984 | 6718059 |
| efficacy of fenbendazole against migrating ascaris suum larvae in pigs. | twenty-four weanling crossbred pigs were inoculated with 10,000 embryonated ascaris suum eggs. the infection was aborted in 1 group (n = 8 pigs) with fenbendazole (3 mg/kg of body weight) given on postinfection days 2, 3, and 4 and in a 2nd group (n = 8 pigs) given fenbendazole at the same rate on postinfection days 6, 7, and 8; a 3rd group (n = 8 pigs) was not treated with fenbendazole. all pigs were necropsied at 24 days after they were inoculated. fenbendazole was 99% effective against a suum ... | 1984 | 6732034 |
| lysophospholipase activation with ascaris suum extract. | | 1984 | 6737160 |
| purification of an anticoagulant from the body fluid of ascaris suum. | an anticoagulant has been purified from the body fluid of ascaris suum by sequential passage through sephadex g-50, cm-cellulose and sephadex g-25 columns then treated with 2 m nacl, passaged through a sephadex g-25 column, separated from the phosphate buffer by precipitation of the latter with the cacl2, then passaged through a sephadex g-10 column in water. in the body fluid of the worm, the anticoagulant is ionically-bound to a carrier substance. the complex can be split by treatment with 2 m ... | 1984 | 6744822 |
| evaluation of fenbendazole as an extended anthelmintic treatment regimen for swine. | fenbendazole was given in the feed to swine at a cumulative dosage of 9 mg/kg of body weight over a period of 3, 6, and 12 days to compare efficacy. four treatment groups of ten 2- to 3-month-old pigs each, with a mean of 15 kg of body weight per group, received 3 mg of fenbendazole/kg/day for 3 days, 1.5 mg/kg/day for 6 days, 0.75 mg/kg/day for 12 days, and no medication. medicated feed was scheduled so that all treated pigs reached the last day of treatment on the same day, thus making the tim ... | 1984 | 6746372 |
| intestinal basement membrane of ascaris suum. properties of the collagenous domain. | | 1980 | 6768727 |