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lung mechanics following antigen challenge of ascaris suum-sensitive rhesus monkeys.pulmonary resistance (rl) breathing air and a helium-oxygen mixture (he-o2) dynamic compliance (cdyn), respiratory frequency (f), subdivisions of lung volume, and static deflation pressure-volume (pv) curves were measured before and after bronchial challenge with ascaris suum antigen in six rhesus monkeys with varying skin reactivity to this antigen. the animals were lightly anesthetized with pentobarbital sodium, intubated, and studied in a volume displacement body plethysmograph, transpulmonar ...1976825504
proteinase activity in ascaris suum eggs, hatching fluid, and excretions-secretions.hatching fluid and the excretions and secretions (e.s.) of hatched larvae of ascaris suum revealed proteinase activity when assayed by 2 different procedures employing collagen or casein as substrates. both assays apparently detected similar levels of proteinase activity in hatching fluid and e.s. of hatched larvae. the anson (casein substrate) assay worked best in 0.05 m phosphate buffer, ph 8.0. the azocoll (collagen substrate) assay worked best in 0.05 m borate buffer at ph 8.8. azocoll assay ...197610365
effect of ascaris extract applied intravenously, on segment bronchus and the influence of ipsilateral vagus blockade.respiratory hypersensitivity and systemic effects after local application of ascaris suum extract on the segmental bronchus and after its intravenous infusion are studied on four boxer dogs. influence of ipsilateral vagus blockade on the respiratory hyperreactivity after intrabronchial application of ascaris was also investigated on the same dogs. ascaris extract was applied in liquid form directly through a catheter into the right segmental bronchus. egg albumin was applied in the same way for ...19767007
nad+-malic enzyme. regulatory properties of the enzyme from ascaris suum.the regulatory properties of the nad-dependent malic enzyme from the mitochondria of ascaris suum have been studied. the malate saturation curve exhibits sigmoidicity and the degree of this sigmoidicity increases as the ph is increased. fumarate was the only compound tested that stimulated the enzyme activity, whereas oxalacetate was the most powerful inhibitor. activation by low levels of fumarate was found to be competitive with malate. it is proposed that this stimulation has physiological si ...1976180002
reaginic antibody production to ascaris suum allergen, asc-1. ii. influence of type of adjuvant and of carrier priming on the induction of ige and igg antibodies to dinitrophenyl conjugates. 1977195904
ige-type antibodies to ascaris antigens in man.ige antibodies to ascaris antigens were detected by radioallergosorbent test (rast) in the serum obtained from a person experimentally sensitized with ascaris suum antigens prepared from the body fluid of worms. this serum passively sensitized human skin in vitro and histamine release from the sensitized skin was observed by challenge with antigens. the allergenicity of a. suum antigens was present over a very broad spectrum of molecular size. the present results suggest that specific ige antibo ...197773520
[antiallergic asthma effect of ipatropium bromide (sch 1000) in dogs (author's transl)].antiasthmatic effect of ipratropium bromide (sch 1000) which is a quarternary ammonium derivative of atropine was investigated in comparison with atropine in anesthetized dogs. asthmatic responses were provoked with inhalation of ascaris suum antigen. sch 1000 (0.1 approximately 0.3%) and atropine (0.1 approximately 0.3%) were given by inhalation for 10 min prior to or after the antigen inhalation. pretreatment of sch 1000 and atropine inhibited the increase in respiratory resistance (rrs) induc ...1977145994
[immunological diagnosis of filariosis in persons returning from tropical countries and in european and indigenous inhabitants of endemic areas (author's transl)].serum samples from persons who lived in areas where onchocerciasis occurred or who had filariasis were examined with the complement fixation test and the indirect hemagglutination test for the presence of antibodies against crude extracts from dirofilaria immitis, onchocerca volvulus, dipetalonema viteae, and ascaris suum. the results could be interpreted as follows: 1. the indirect hemagglutination test was more sensitive than the complement fixation test for the demonstration of antibodies in ...1977271633
[endoparasites of calves in large herds].the authors present preliminary results of the coprological examination of calves in large-capacity calf-houses. in a new large-capacity calf-house in the benesov district in the central-bohemian region, 200 samples of faeces were examined according to breza (1957) and further examinations were performed regularly in a group of 42 calves from the age of 0.5 to 6 months. the tests revealed 13 species of endoparasites, including eight species of coccidia: eimeria auburnensis, e. alabamensis. e. bo ...1977413242
ascaris suum: immune response in the guinea pig. i. lymphoid cell responses during primary infections. 1977320026
ascaris suum: location of phosphorylcholine in lung larvae. 1977330187
toxocaral larva migrans: the use of larval secretory antigens in haemagglutination and soluble antigen fluorescent antibody tests.toxocara larval excretions and secretions collected from in vitro culture were used as antigen in passive haemagglutination and soluble antigen fluorescent antibody tests for the diagnosis of visceral larva migrans in experimental animals and man. antibody to toxocaral secretions was detected in rabbits within 13 days of light toxocara infection (ten larvae per kg) and within four days of heavy infection (10(4) larvae). antibody was not detected following infection with 10(4) ascaris suum larvae ...1977343311
ascaris suum: cuticular binding of the third component of complement by early larval stages. 1977403088
reaginic antibody production to ascaris suum allergen, asc-1. i. the function of glutaraldehyde-polymerized antigen in the induction of reaginic (ige) antibodies in the rat.rats immunized with a purified ascaris suum allergen (asc-1) or with its dinitrophenylated derivate (dnp-asc-1) produced high levels of reaginic (ige) antibodies. a second injection of antigen given 30 days later did not result in an anamnestic ige antibody response. immunization of adult-thymectomized, lethally-irradiated and bone-marrow reconstituted (atxb) rats with soluble asc-1 or dnp-asc-1 failed to stimulate reaginic antibody production. the administration of glutaraldehyde-polymerized an ...1977301862
ascaris suum: mitochondrial dna in fertilized eggs and adult body muscle. 1977558903
development of ascaris suum eggs. 1977560080
the immune response and acquired resistance to ascaris suum infection in mice with an x-linked b lymphocyte defect. 1977303286
ascaris suum: lymphoid cell responses during secondary infections in the guinea pig. 1977321239
toxocara infection in pigs. the use of indirect fluorescent antibody tests and an in vitro larval precipitate test for detecting specific antibodies.an in vitro larval precipitate test using second-stage toxocara canis larvae and an indirect fluorescent antibody (ifa) test employing cuticles of t. canis larvae as antigen were evaluated using antisera produced in pigs experimentally infected with t. canis, t. cati, ascaris suum, toxascaris leonina and parascaris equorum. the former test was both specific and sensitive and is suggested as a reliable and simple method of detecting toxocara antibodies in pigs. the latter test was considered unsu ...1977328560
airway mucosal permeability in the ascaris suum-sensitive rhesus monkey.the permeability of the airways to technetium 99m-labeled albumin was measured in ascaris suum-sensitive rhesus monkeys. all 8 animals were skin-sensitive to ascaris suum (aa) antigen, 4 being respiratory responders (r) and 4 nonresponders (nr) to aerosolized antigen. in the absence of antigen challenge there were no differences in the accumulation in the blood of radioactive material from the tracheobranchial tree between the r and nr animals. after a five-minute challenge with aerosolized aa, ...1977406301
allergy induced asthma with ascaris suum administration to dogs. 1977407388
involvement of histamine h1- and h2-receptors in induced asthmas in dogs.participation of histamine h1- and h2-receptors in both asthmas, i.e. experimentally induced bronchoconstriction and bronchosecretion, with ascaris suum and histamine in anesthetized dogs was investigated. dogs given 0.2% histamine solution or ascaris antigen (3 mg protein) by inhalation showed increases in respiratory resistance (rrs) and respiratory rate to 2.5-5.0 fold. airway secretion volume was also significantly increased 3-4 fold. the increase in rrs by histamine inhalation was effective ...197724777
haemagglutination tests in the study of ascaris epidemiology.haemagglutination tests using adult ascaris suum antigen were performed on sera from 810 people from three areas in papua new guinea and east timor. in the area with the highest childhood ascaris infection rates and egg-counts (kaul), titres were highest and remained high throughout life. there was a significant negative correlation between titre and eosinophil count, and it is suggested that high titres in adult life result from continuous usually unsuccessful reinfection, and that the declinin ...197717370
rapid, shallow breathing after ascaris suum antigen inhalation: role of vagus nerves.in five treadmill-exercising, unsedated dogs, we studied the effect of inhaled ascaris suum antigen aerosols on minute volume of ventilation (ve), respiratory frequency (f), tidal volume (vt), total pulmonary resistance (rl), and dynamic pulmonary compliance (cldyn), before and during cooling of the vagus nerves. with the vagi warm, inhaled antigen increased ve (mean + 62%; p less than 0.01)by increasing f (mean + 180%; p less than 0.01), despite a decrease in vt (mean - 42%; p less than 0.01). ...197713058
the difference in effect of predosing with antiallergic compounds between the rat pca model and the monkey asthma model. comparison of the effects of prd-92-ea and disodium cromoglycate.dscg and prd-92-ea showed tachyphylaxis in the rat pca test when a large intravenous dose (40 mg/kg of dscg and 20 mg/kg of prd-92-ea) was given 30 min before administering intravenously the expected effective doses at the time of antigen challenge. this predosing led to a marked loss of effectiveness. cross-reacting tachyphylaxis was also demonstrated in this model. predosing rats with dscg led to a great loss of effectiveness of a subsequent expected active dose of prd-92-ea and predosing with ...1977412794
immune mechanisms to ascaris suum in inbred guinea-pigs. i. passive transfer of immunity by cells or serum.syngeneic strain 2 guinea-pigs which received seven s.c. injections of infective eggs of ascaris suum were shown to harbour less larvae in their lungs than control animals after mesenteric vein challenge with 10,000 infective larvae of a. suum. serum and cell preparations harvested from these animals were able to transfer protective immunity to normal recipients to varying degrees. significant protection with serum preparations was obtained with immune igg2, ige + igg1 and whole immune serum. be ...1977608676
ascaris suum: hatching of embryonated eggs in swine. 1977560316
destruction of ascaris suum eggs during their feeding to various species of beetles.eggs of ascaris suum (linné, 1758) were fed to nine species of beetles of the families scarabacidae, carabidae and histeridae in order to ascertain the dependence of egg penetration on the structure of mouth parts of the beetle. the mouth parts of some beetle species have various filtration apparatuses preventing the helminth eggs from penetrating into the digestive tract. geotrupes stercorosus (scriba) was found to possess a crushing apparatus which destroys a great number of helminth eggs pene ...1977564316
infection of weanling pigs with known numbers of ascaris suum fourth stage larvae.nineteen weanling pigs were each infected per os with 200 fourth stage ascaris serum larvae and were fed either a low protein or a high protein diet for 8 weeks. worms expelled during the 8 weeks were recovered and all worms remaining in the gastrointestinal tract at slaughter were collected. fifty-four percent of all worms were recovered at slaughter. ten of the 19 animals maintained an infection for 8 weeks; the other 9 showed no signs of a. suum infection. a total of 377 worms were recovered. ...1977832477
study of the pulmonary circulation in a canine asthma model.measurements of respiratory mechanics, gas exchange, and pulmonary hemodynamics were made before and serially for 2 hours after inhalation challenge with ascaris suum extract in dogs with natural sensitivity to this antigen. nine of 14 dogs randomly exposed to an inspired oxygen fraction of 0.21 or 0.60 to 0.70, responded to the challenge with bronchospasm reflected by a transient decrease of mean specific respiratory system conductance to 50 per cent of baseline after 5 to 15 min. this was acco ...1977842939
critical evaluation of oxfendazole as a swine anthelmintic.sixty feeder pigs with mixed naturally acquired nematode infections were medicated with oxfendazole, a new benzimidazolecarbamate compound, at dose levels of 0, 3, 9, and 18 mg/kg of body weight. the dosage of 3.0 mg/kg administered once in the feed proved as efficacious as larger dosages and the same dosage administered 2 or 3 times in the removal of adult ascaris suum, oesophagostomum dentatum, and metastrongylus pudendotectus. trichuris suis adults were less susceptible. anthelmintic efficacy ...1977851278
intestinal basement membrane of ascaris suum. analysis of polypeptide components. 1977863911
intestinal basement membrane of ascaris suum. characterization of carbohydrate units.characterization of glycopeptides obtained on alkaline hydrolysis and on extensive collagenase and pronase digestion of the intestinal basement membrane showed the existence of two distinctly different carbohydrate units. one of these is a disaccharide, composed of glucose and galactose, linked to hydroxylysine. it was shown to be identical to the unit (2-o-d-glucopyranosyl-o-d-galactopyranosylhydroxylyasine) present in vertebrate basement membranes, as determined from stability to alkaline hydr ...1977863912
a new allergen from the perienteric fluid of ascaris suum with respect to charges.a new allergen with pi in the 6.0 region was obtained from the perienteric fluid of ascaris suum through isoelectric focusing techniques. this new allergen shows strong allergenicity, at a concentration of 10(-9) microgram/ml, in rats passively sensitized with the reaginic antiserum produced against ascaris perienteric fluid.1977873628
development of larvae of ascaris suum from the third to the fourth stage in a chemically defined medium. 1977873685
eosinophilia and mast cell hyperplasia induced by parasite phospholipid.phospholipid preparations from the nematode ascaris suum or cysts of echinococcus granulosus (hydatid cysts) induced an eosinophilia when injected into the peritoneal cavity of rats. peritoneal eosinophilia persisted throughout 21 days of daily injections of ascaris lipid and was accompanied by blood eosinophilia, mast cell granule lysis and mast cell hyperplasia. the active material consisted of lecithin and lecithin plasmalogen, and in aqueous suspension had a membrane-like appearance. electro ...1977876690
changes in airway mast cells and histamine caused by antigen aerosol in allergic dogs.we studied the effect of inhaled specific antigen on airflow resistance, histamine concentration, and mast cell numbers in airway tissues in allergic dogs. in each of six allergic dogs with open chests, inhalation of specific antigen aerosol (ascaris suum) increased airflow resistance from 0.30+/-0.40 (mean+/-se) to 2.8+/-0.41 cmh2o/1 per s (p less than 0.05); decreased mast cell number from 38.6+/-3.2 to 24.3+/-4.5 mast cells/mm2 (p less than 0.05); decreased histamine (per mg airway tissue) fr ...1977893281
intraocular ige antibody induced in guinea pigs with ascaris suum larvae.the systemic and local (intraocular) ige antibody responses to infective and heat-killed ascaris suum larvae were examined in guinea pigs. ige antibody, demonstrated by the 6-day p-k test, was found in anterior chamber fluid following primary intravitreal injection of live second-stage a. suum larvae and in some instances occurred when no serum p-k activity was demonstrable. no intraocular ige antibody was induced by primary intravitreal injection of heat-killed larvae. a brisk intraocular and s ...1977908652
reactivity and specificity of trichinella spiralis fractions in cutaneous and serological tests.six diethylaminoethyl-cellulose fractions of a larval trichinella spiralis extract, an ascaris suum extract, and a nonrelated protein were used for cutaneous tests in guinea pigs with 8-, 14-, and 73-day-old t. spiralis infections, in guinea pigs with 13-day-old a. suum infections, and in normal guinea pigs. a selected t. spiralis fraction was used in hemagglutination (ha) tests with sera of 8 t. spiralis-infected rabbits, 41 sera of trichinellosis patients positive by bentonite agglutination te ...1977908745
an ultrastructural study of the invasion of ascaris suum larvae by neutrophils. 1977908839
[studies of the decontamination of the sewage from animal husbandry farms].investigations revealed that under the conditions prevailing in the districts of sofia and .pernik ascaris suum and trichocephalus suis eggs remained viable at the conventional storage of liquid manure on the large farms for about a year, and fasciola hepatica and paramphistomum sp. eggs--for 2 to 3 months. the methods of electrocoagulation and electroflotation could be employed with success in the cleansing and rendering harmless of the sewage waters after the preliminary treatment with calcium ...1977919336
[studies of the corticosteroid hormones in the nematode, ascaris suum].four steroids from tissues of ascaris suum were isolated and identified by means of the thin-layer chromatography and fluorimetric methods as corticosterone, cortisol, alodosterone and dehydrocorticosterone. corticosterone, cortisol and alodosterone are corticosteroid hormones while dehydrocorticosterone is a product of a metabolic transformation of corticosterone. it seems likely that the presence of corticosteroid hormones in tissues of a. suum is indicative of their participation in the metab ...1977896275
[bronchomotoric in antigen induced airway obstruction (author's transl)].ascaris suum extract inhalation causes bronchoconstriction in dogs. following this reaction, the bronchi stay oversensitive against acetylcholine for at least 7 h. electrical stimulation of the peripheral end of a nervus vagus after cutting supports the meaning that the bronchoconstriction will be caused mainly by challenge of sensory receptors. the reflex way runs through the nervus vagus. sensitization of the bronchial tree means sensitization of the sensory receptors. results of the arterial ...1977897366
elimination of ascaris suum eggs from sewage by air flotation. 1977917786
anatomy of the intestine of ascaris suum: a three dimensional study with silicone rubber casts. 1977884638
proteolytic enzymes and enzyme inhibitors in ascaris suum. i. proteolytic enzymes and enzyme inhibitors in the gut. 1977754545
proteolytic enzymes and enzyme inhibitors in ascaris suum. ii. proteinase inhibitors produced in vitro in enzyme-free medium. 1977754546
proteolytic enzymes and enzyme inhibitors in ascaris suum. iii. in vitro study of proteinase inactivation by the parasite. 1977754548
ascaris suum: immunization with soluble antigens in the guinea pig. 1977924714
an evaluation of ethyl-6-ethoxybenzothiazole-2-carbamate (sch 18099) for anthelmintic activity in domestic animals.ethyl-6-ethoxybenzothiazole-2-carbamate (sch 18099) was evaluated for efficacy against natural helminth infections in ponies, pigs, lambs and chickens. sixteen critical trials were conducted in ponies at dosages of 15 to 150 mg/kg. at 15 mg/kg, efficacy against adult and larval oxyuris equi was 100% and 91% and against small strongyles it was 98%. efficacy levels were 95% against strongylus vulgaris and s. edentatus at the 20 mg/kg dosage. in two trials at 100 mg/kg efficacy against parascaris e ...1977864223
formation of cytoplasmic synaptonemal-like polycomplexes at leptotene and normal synaptonemal complexes at zygotene in ascaris suum male meiosis. 1977885026
ascariasis: the development of eggs of ascaris suum under the conditions prevailing in a pig house.eggs were collected from gravid female ascaris suum in the abattoir. cultures were prepared with the eggs in 2 to 3 mm depth of 1 per cent sulphuric acid or on the surface of 2% aqueous agar. they were allowed to develop in a piggery, fresh cultures being started at intervals from january to september. eggs on the surface of agar developed failry uniformly and a high proportion eventually reached l2. in contrast those under 1% sulphuric acid were less uniform, slower in development and generally ...1977878244
[a study on the lipids of ascaris suum ova]the present study was undertaken to observe the quality and quantity of lipid constituents by the developing ascaris suum eggs. the collected eggs from the uterus of a. suum were classified into 3 groups, i.e., single cell stage, morula stage and embryonated eggs, and were subjected to analyse their lipid fractions. to obtain the morula stage eggs, 10 to 11 incubation days at 30 degree c were needed and for the embryonated eggs, 30 to 31 days were lasted. at the time of experiment, their indices ...197712913434
experimental infection of collared peccary ( dicotyles tajacu angulatus ) with swine kidney worm ( stephanurus dentatus ).two captive-born juvenile collared peccaries ( dicotyles tajacu angulatus ) were given 3000 infective larvae of stephanurus dentatus per os. one peccary harbored viable s. dentatus sub-adults in the liver 50 days post-infection. the other peccary had no larvae but did have diffuse fibrotic hepatic lesions and bile duct hyperplasia 213 days post-infection; however, the lesions may have been partially due to a concurrent ascaris suum infection. a domestic pig ( sus scrofa domesticus ) infected as ...197724228971
analysis of immunosuppression during early acute infection of mice with ascaris suum.immunosuppression was examined at 10 to 12 days following oral inoculation of 10,000 to 12,000 embryonated ascaris suum eggs. reduced antibody responses to sheep red cells (src) following systemic immunization were confirmed in cd-1 and c57bl/6 mice. infection alone induced antibody reactive with dnp equivalent to that observed after immunization with dnp--ficoll. there was a decrease in thymus and spleen size by day 8 of infection, followed by a splenic proliferative response during the second ...1978709911
[effect of several cholinomimetic and anticholinesterase substances on the somatic musculature of the nematode ascaris suum].on the basis of discrete concentration--effect curves, quantitative evaluation of the effect of acetylcholine, propionylcholine, suberylcholine and carbacholine upon the strips of the spinal muscle of ascaris was made. anticholinesterase drugs, eserine and phosphacol, exhibit weak potentiation of the effect of acetycholine (by 2--6 times). the results obtained are discussed in relation to cholinoreceptive properties of muscles in higher animals.1978735601
the spin method for recovering tissue larvae and its use in evaluating c57bl/6 mice as a model for the study of resistance to infection with ascaris suum.a centrifugation method for recovering tissue larvae is described and is proven to be superior to both baerman and tissue digest methods for recovering larvae of ascaris suum from the liver and lungs of infected c57bl/6 mice. using the spin method for harvesting larvae, c57bl/6 mice were evaluated for their suitability in studies on specific host immunity to infection with a. suum.1978739293
ascaris suum: free amino acids and proteins in the pseudocoelom, seminal vesicle, and glandular vas deferens. 1978680082
h2-receptors in ascaris suum [proceedings]. 1978685773
gas exchange during acute experimental canine asthma.the inert gas infusion technique was used to recover distributions of ventilation-perfusion ratios in anesthetized dogs during 17 episodes of acute asthma produced experimentally by broncho-provocation with aerosolized ascaris suum extract, methacholine, or histamine. regardless of the agent used, 2 general patterns of ventilation/perfusion maldistribution were observed. during mild attacks of bronchospasm, a broadening of the normal unimodal distribution of ventilation-perfusion ratios occurre ...1978707880
comparative efficacy of fenbendazole, dichlorvos, and levamisole hci against gastrointestinal nematodes of pigs.forty-two weanling pigs raised under similar management conditions were used to compare the effectiveness of fenbendazole, dichlorvos, and levamisole hcl against oesophagostomum dentatum, o. quadrispinulatum, ascaris suum, trichuris suis, hyostrongylus rubidus, and strongyloides ransomi. all three drugs were given in the feed. eleven pigs served as unmedicated controls; 11 pigs received fenbendazole (3 mg/kg) on days 1, 2, and 3; 10 pigs received dichlorvos (17 mg/kg) on day 3; and 10 pigs recei ...1978739296
new method for evaluating bronchomotor and bronchosecretory activities: effects of prostaglandins and antigen.we devised a new method for measuring responses of canine airway musculature, bronchosecretion and bronchial vasculature simultaneously, in situ, and investigated the effects of prostaglandins f2alpha and e2 and antigen-antibody reaction by using this model. the right bronchial artery was perfused with blood at a constant flow under artificial respiration. airway musculature response was measured as a change in ventilation overflow with a modification of the konzett-rössler method; the airway se ...1978702943
parasites of domestic and wild animals in south africa. vii. helminths in pigs slaughtered at the pretoria municipal abattoir.a total of 52 pigs slaughtered at the pretoria municipal abattoir over a period of 1 year was examined for parastitic helminths. twenty-six of these pigs were marketed by farmers and 26 by speculators. of the pigs marketed by farmers 73,1% were found to be infested:--30,8% with ascaris suum, 65,4% with ascarops strongylina, 3,8% with metastrongylus apri, 26,9% with oesophagostomum spp., 15,4% with trichostrongylus colubriformis and 15,4% with trichuris suis. all the pigs marketed by speculators ...1978568230
parasites of domestic and wild animals in south africa. viii. helminths in pigs kept under semi-intensive conditions.the seasonal incidence of nematode infestations in pigs raised under semi-intensive conditions was determined by the monthly slaughter of 2--4 tracer pigs exposed to infestation in an earthernfloored pen for periods of 1 or 2 months. although worm burdens were generally small, ascarops strongylina appeared to be more prevalent from november to march than during the other months of the year. once ascaris suum became established in the pen nearly all the pigs became infested. the number of worms n ...1978568231
[effect of ensilaging the solid fraction of dungfly on the eggs of ascaris suum and fasciola hepatica]. 1978569926
caenorhabditis elegans and ascaris suum: fragmentation of isocitrate lyase in crude extracts. 1978627278
influence of low concentrations of allergens on bronchial system.the influence of ascaris extract, applied during 1 h in low concentration as aerosol and intravenously, on the bronchial system and the potentiation of increase of airway resistance to acetylcholine (ach) and histamine challenge caused by this kind of allergen exposure were studied in 13 dogs. all the animals presented no reaction by the allergen exposure itself but developed an increased response to ach and histamine after this kind of allergen exposure. a control group of 4 dogs was submitted ...1978622524
contribution to the findings of eggs of ascaris suum goeze, 1782 in calves kept in the large-scale calf houses. 1978689563
glutathione conjugation of some xenobiotics by ascaris suum and moniezia expansa.1. the cestode moniezia expansa and the nematode ascaris suum both possess enzymes catalysing the conjugation of glutathione (gsh) with 1-chloro-2,4-dinitrobenzene. 2. the gsh-s-aryl transferase (gsh-s-transferase a) was present in the cytosol of the cestode proglottids and of the nematode intestinal epithelial cells. other tissues did not contain measurable activity. the enzymes from both species had mol. wt. of about 37 000 and broad ph optima around ph 8.3. both enzymes were inhibited by cu2+ ...1978654311
lack of homocytotropic antibody potentiation by ascaris suum infection in pigs. 1978654701
isolation of ascaris suum eggs for experimental purposes. 1978655024
caenorhabditis elegans and ascaris suum: inhibition of isocitrate lyase by itaconate. 1978658222
failure of hypoxic pulmonary vasoconstriction in the canine asthma model. effect of prostaglandin inhibitors.measurements of respiratory mechanics, arterial blood gases, and pulmonary vascular resistance were made before and 15 min after inhalation challenge with ascaris suum extract in dogs with natural sensitivity to this antigen. 25 of 47 dogs were treated before inhalation challenge with a prostaglandin inhibitor (90 mg/kg of aspirin or 2 mg/kg of indomethacin by intravenous infusion). in response to the challenge, bronchospasm developed in approximately half (responders) of each group reflected by ...1978659609
the role of complement in ascaris suum induced histopathology.the role of complement in the histopathology of primary and challenge re-infection by ascaris suum was assessed. guinea-pigs deficient in c4 or depleted of the terminal components c3 to c9 by cobra venom factor (cvf) were employed. pathological changes in the livers of complement-deficient guinea-pigs differed slightly from controls. the most striking findings were in the lungs of cvf-treated infected animals, where marked eosinophilic abscesses were observed following either a primary or repeat ...1978668193
respiratory mechanics in conscious sheep: response to methacholine.most currently used animal models of allergic airway diseases differ from human asthma in that induced bronchospasm in the former is not accompanied by pulmonary hyperinflation. in the present investigation, we chose unsedated, restrained sheep to determine the effect of cholinergic bronchial provocation on respiratory mechanics, functional residual capacity (frc), and arterial blood gases. seven animals had been actively sensitized by intramuscular injections of ascaris suum extract, and four u ...1978632189
[facts that explain the host specificity of helminths].extracts from larvae of trichinella spiralis, mature ascaridia galli and ascaris suum and blood sera of their possible hosts (rats, pigs, chicks, guinea pigs, mice and men) were investigated by the method of electrophoresis in polyacrylamide gel. the protein component was found to be most identical in helminth and its obligate host. the host specificity is supposed to depend on the community of the protein content of the parasite and its host. the data on the difference in protein spectra are gi ...1978643309
[parasites in the gdr.1. studies on helminths occurrence in european wild pigs (sus scrofa)].in two different hunting-grounds in the north-eastern part of the gdr from november 1972 to march 1974 the authors tested a series of 102 killed wild boars, aged mostly from 7 month to 3 years by means of helminthological dissection and by coprological methods for helminthic stages. from more than 30 well known species of helminths the following 10 species were found: cysticercus tenuicollis, echinococcus hydatidosus, ascaris suum, ascarops strongylina, physocephalus sexalatus, globocephalus uro ...1978646175
immunization of pigs against experimental ascaris suum infection by feeding ultraviolet-attenuated eggs.pigs fed ascaris suum eggs attenuated by short-wave ultraviolet radiation developed a resistance to challenge infections that varied according to the protocols used. two doses of eggs irradiated at total exposures (et) of 150 muw-min/cm2 resulted in a reduction of adult worm burden in 3 groups challenged with 50, 100, or 500 eggs by 45, 35, and 49%, respectively. however, these results were not statistically significant (p greater than 0.05). three other groups were fed eggs as follows: group 1, ...1978682067
esophagogastric ulcers associated with ascaris suum infestation in swine.when a group of 3-month-old pigs was moved to another location, several died from internal bleeding. two pigs that were necropsied had large esophagogastric ulcers, hepatic fibrosis with "milk spots" and swollen edematous lungs. the ulcers involved the full thickness of the gastric mucosa with pronounced eosinophilic infiltration and perivascular cuffing of the submucosal vessels. there was an acute interstitial and granulomatous pneumonia with an inflammatory exudate composed mainly of eosinoph ...1978685083
intestinal obstruction due to ascaris suum infection. 1978635969
factors in the movement of hexose across the intestine of ascaris suum.trichloroacetic acid extractable carbohydrate of the intestine of ascaris suum decreases rapidly when ribbons of the tissue are incubated in a basal salt solution. after 10 min incubation endogenous carbohydrate is 32% and after 80 min it is 19% of the "zero" time control value. in contrast, there is approximately a 2-fold increase in the endogenous carbohydrate of tissue that is incubated in saline with glucose. the increase occurs within the first 5 min and is maintained throughout an 80-min i ...1978641659
molecular characterization of ascaris suum dna and of chromatin diminution. 1978568561
ascaris suum: specific antibodies in isolated intestinal loop washings from immunized swine. 1978354957
the occurrence of the second moult of ascaris lumbricoides and ascaris suum. 1978571857
[b-lymphocyte rosette formation phenomenon in the spleen of mice infested with ascaris suum larvae]. 1978308152
[fractionation of the trichinella spiralis antigen and allergy diagnosis trails].experiments were carried out to fractionate an antigen of trichinella spiralis with sephadex g-50 and g-100. the specificty of the fractions has been followed up by means of the intradermal allergic reaction in six pigs, experimentally infected with trichenalla spiralis, one with trichocephalus suis and three, born by trichinellosis-affected sows. the second fraction has been tested also on four pigs, experimentally inoculated with ascaris suum, nine--with ascarsis suum and oesophagostomum and 1 ...197888790
purification and characterisation of two proteins from ascaris suum extract, antigenically different but bearing common allergenic epitopes.two proteins, fi and fvii, have been purified from a whole worm extract of adult ascaris suum, using ammonium sulfate precipitation, sephadex gel filtration and deae cellulose chromatography. both of them provoke reagin formation in mice as tested in presensitized animals with an original immediate hypersensitivity reaction test. they are two chemically and physically different protein molecules showing no cross reaction with a rabbit serum anti-crude extract but bearing common allergenic epitop ...197878891
inhibition of allergic reactions by cromoglycate and by a new antiallergy drug u-42,585e. ii. activity in primates against aerosolized ascaris suum antigen.immediate type hypersensitivity to ascaris antigen in rhesus reactor monkeys was used to assess the pharmacologic profile of cromolyn na (dscg) and a new inhibitor of ige-mediated lung function changes (u-42,585e). two parameters used to measure lung function, respiratory rate increase and tidal volume decrease, were significantly altered in a dose-related fashion by u-42,585e when the latter was administered either by the intravenous, intrabronchial, or the oral route. oral activity of u-42,585 ...1978415987
[effectiveness of antigens from various developmental stages of ascaris suum in the deomonstration of the migratory phase of experimental ascariasis in pigs].tests of the efficiency of antigens prepared from different developmental stages of ascaris suum in indirect haemagglutination test in the course of proving the migration phase of experimental ascariasis in pigs show that the antigens prepared by ultrasound from the invasive larval stage of a. suum in comparison with antigens of sexually mature stages have higher serological activity. by using this antigen it is possible to prove specific antibodies in experimentally invaded pigs from 6 to 120 d ...197896575
isolation and partial characterization of trehalase from ascaris muscle.the tissues of female ascaris suum were assayed for alpha,apha'-glucoside 1-d-glucohydrolase (trehalase) activity. a soluble from of the enzyme was isolated from muscle tissue and purified approximately 37-fold. the enzyme was specific for trehalose as substrate. the ph optimum for enzymatic activity was found to be 6.0, and the apparent km for trehalose was estimated to be 2.1 x 10-4 m. the product of the reaction was identified as d-glucose by chemical, chromatographic and enzymatic methods.197828390
effect of ultraviolet radiation on the infective stages of ascaris suum and stephanurus dentatus with a comparison of the relative susceptibilities of some parasitic nematodes to ultraviolet. 1978147934
aldose reductase and sorbitol dehydrogenase in the muscle of ascaris suum (nematoda).the muscle of ascaris suum contains sorbitol dehydrogenase [ec 1.1.1.14] and the apparent km values indicate that the enzyme favours fructose formation. aldose reductase [ec 1.1.1.21] is also present and like the mammalian enzyme it has a very high apparent km for glucose. possible functions of the sorbitol pathway in ascaris muscle are discussed.1978116186
[comparison of the sensitivity of the passive, indirect hemagglutination reaction and the latex fixation test in the demonstration of the migratory phase of experimental ascariasis (ascaris suum) in swine].the authoresses verified diagnostic sensitivity of two serological tests -passive indirect hemagglutination [iha] reaction modified by the authoresses and latex-fixation test [lft] when proving the post-invasion migratory phase of ascariasis [ascaris suum] in experimentally infected swine. the iha reaction was found to be more sensitive than the lft in most cases [62.2%] by two to three values of the geometric series. the invasion can be detected by the iha reaction from 7th to 120th day and by ...197897840
airway responses to histamine and methocholine in ascaris suum-allergic rhesus monkeys.we performed dose-response curves to inhaled histamine (h) and methacholine (mc) in a group of eight rhesus monkeys, with and without natural allergy to ascaris suum antigen (aa). the animals were anesthetized with pentobarbital sodium, 25 mg/kg im and studied in a volume-displacement body plethysmograph. the dose of h or mc producing a 50% increase in pulmonary resistance (rl) was used to determine sensitivity to these agents and the increase in rl at a given dose was employed as a measure of r ...1978103872
kinetic and isotope partitioning studies on the nad+-malic enzyme from ascaris suum. 1978204633
the effects of the antiparasitic drugs levamisole, thiabendazole, praziquantel, and chloroquine on mitochondrial electron transport in muscle tissue from ascaris suum. 1978203843
effect of pyrantel tartrate and carbadox on acquisition of the swine kidneyworm (stephanurus dentatus) and other parasites by pigs on contaminated lots.a combination of pyrantel tartrate (106 mg/kg of body weight) and carbadox (55 mg/kg of body weight) in ground feed was fed to 20 weaned pigs (av wt, 14.4 kg) for 42 days. another group of 20 pigs included nontreated controls. the pigs were farrowed and suckled in a slat-floored farrowing house and had minimal exposure to the small intestinal threadworm (stronglyoides ransomi) until they were placed on severely contaminated dirt lots at the start of the experiment. five pigs from each of the two ...1979160763
bronchial mucosal permeability.the tracheobronchial epithelium has well-developed tight junctions which on a morphologic basis should be markedly resistant to penetration by protein molecules. despite this, antigen inhalation in monkeys allergic to ascaris suum results in the rapid onset of pulmonary physiologic changes. recent studies in man and animals have shown that a substantial number of mast cells exist in the bronchial lumen and epithelium. we suggest that antigen-antibody interaction initially occurs on these superfi ...1979104888
differentiated b lymphocytes. potential to express particular antibody variable and constant regions depends on site of lymphoid tissue and antigen load.b cells have the potential to respond to an antigen by producing antibodies with a variety of variable and constant regions. we have quantitatively analyzed b-cell potential at the single cell level to determine the effect of lymphoid tissue site and antigen load on the expression of variable and constant regions. concerning variable region expression, although the total frequency of b-cell precursors for phosphorylcholine is similar between nonimmune spleen and gut-associated peyer's patch tiss ...1979105075
airway sensitivity to slow-reacting substance of anaphylaxis, histamine, and antigen in ascaris sensitive monkeys.the effects of ascaris suum antigen, histamine, and slow-reacting substance of anaphylaxis (srs-a) on the respiratory system were compared in 3 anesthetized rhesus monkeys. the agents were administered by instillation into the trachea, and the animals were studied in a volume displacement body plethysmograph. two of the animals showed skin and bronchial sensitivity to ascaris suum antigen and responded to it with increased pulmonary resistance and decreased dynamic compliance. a similar response ...197936017
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