| trichinella spiralis: a 76-kda excretory/secretory larval antigen identified by a monoclonal antibody. | spleen cells from balb/c mice were fused with myeloma cells following infection of the mice with trichinella spiralis larvae and an ip booster injection with larval homogenate antigen. a monoclonal antibody (mab), designated as ts 3g6 which did not react with sera or tissue extracts from noninfected mice, rats, and guinea pigs, was selected for further studies because of its high activity and specificity. when tested in elisa ts 3g6 did not cross-react with ascaris suum, a. lumbricoides, toxocar ... | 1991 | 1889470 |
| trichinella spiralis: light microscope monoclonal antibody localization and immunochemical characterization of phosphorylcholine and other antigens in the muscle larva. | a panel of monoclonal antibodies was used to examine the structure of the muscle larva of trichinella spiralis under the light microscope. immunofluorescence and, in some cases, immunoperoxidase staining were used. all four antibodies reacted with the cuticle of the organism, although differences in the staining pattern were observed for some of these. interestingly, all the antibodies also reacted with the stichosome. one of the antibodies (ts2ab) is specific for the hapten, phosphorylcholine. ... | 1991 | 1889472 |
| a seroepidemiologic survey of human dirofilariosis in western spain. | the objective of this work is to define the prevalence of igm and igg antibodies against dirofilaria immitis in an exposed human population. 395 individuals attending primary care facilities in an area endemic with canine dirofilariasis were studied, as well as 100 non exposed individuals. an elisa method was used for the detection of igg and igm antidirofilarial antibodies. sera were preadsorbed with antigens of toxocara canis, ascaris suum, echinoccocus granulosus and fasciola hepatica to prev ... | 1991 | 1896765 |
| airway blood flow modifies allergic airway smooth muscle contraction. | we tested the hypothesis that airway perfusion modifies the contractile response of airway smooth muscle to allergen challenge by influencing the clearance of locally released spasmogens. in six intact, lightly sedated, sheep allergic to ascaris suum, we measured tracheal mucosal blood flow (qtr) with a soluble gas uptake method and tracheal dead space (vtr), an index of airway smooth muscle tone, by helium dilution before and serially after local aerosol challenge with a. suum extract or ragwee ... | 1991 | 1905892 |
| [livestock manure as a vector for parasites--a report of experiences]. | analysing the results of own investigations and informations in literature a review is given about the role of manure specially in stables of high-intensity cattle and pig production as a vector for exogenic parasite stages. in the course of investigations--in most cases simulating practice conditions--so-called indicator germs were used as test material. in cattle stables manure is significant as a vector for eimeria species, cryptosporidium parvum, sarcocystis species, taenia saginata and fasc ... | 1991 | 1914947 |
| molecular symmetry and arrangement of subunits in extracellular hemoglobin from the nematode ascaris suum. | the arrangement of subunits and molecular symmetry of extracellular hemoglobin from the nematode ascaris suum, an 11.7s molecular of molecular mass 332 kda and composed of eight identical subunits, was studied. dissociation of the molecule at alkaline and acid ph yielded 4.6s and 2.7s components, identified as polypeptide-chain dimers and monomers, respectively. cross-linking with glutardialdehyde followed by sds/page resulted in a maximum number of eight bands identified in order of decreasing ... | 1991 | 1915360 |
| ascaris suum: stage-specific differences in lectin binding to the larval cuticle. | a glycosylated component with affinity for wheat germ agglutinin (specific binding to n-acetyl-d-glucosamine monomers and oligomers) and weak affinity for poke weed mitogen (specific binding to n-acetyl-d-glucosamine oligomers) was detected temporally on the surface of ascaris suum larvae developing in vitro and on in vivo-derived larvae. the component was identified on the surface of in vitro-derived late second stage larvae, on all late third stage larvae (derived from pig lung), and all fourt ... | 1991 | 1915752 |
| the pharmacology of cholinoceptors on the somatic muscle cells of the parasitic nematode ascaris suum. | 1. acetylcholine (ach) elicited depolarization and an increase in input conductance of the somatic muscle cells of the parasitic nematode ascaris suum. 2. the relative potency of nicotinic and muscarinic agents was studied in this preparation. the order of potency of these compounds was metahydroxy-phenylpropyltrimethylammonium (hppt) greater than 1.1 dimethyl-4-phenylpiperazinium (dmpp) greater than ach greater than carbachol greater than nicotine greater than tetramethylammonium (tma+) greater ... | 1991 | 1919416 |
| t-cell-dependent accumulation of eosinophils in the lung and its inhibition by monoclonal anti-interleukin-5. | the transnasal administration of an extract of the parasite ascaris suum to c57bl/6 mice for 3 weeks produced marked eosinophilia in the bronchoalveolar lavage (bal) fluid. the oral administration of ciclosporin significantly suppressed the pulmonary eosinophilia. athymic c57bl/6-nu/nu mice failed to develop pulmonary eosinophilia. these data indicate that the pulmonary eosinophilia caused by this parasite extract is t-cell-dependent. genetically mast-cell-deficient (wb x c57bl/6) f1-w/wv (w/wv) ... | 1991 | 1937869 |
| metabolism and fate of ecdysteroids in the nematodes ascaris suum and parascaris equorum. | when injected with [3h]ecdysone and maintained in vitro, the parasitic nematodes, ascaris suum and parascaris equorum each produced a series of polar and relatively apolar metabolites. a. suum metabolised the compound into ecdysonoic acid ([3h]eoic), ecdysone 25-glucoside ([3h]e25gluc), putative ecdysone 22-phosphate ([3h]e22p) and a series of at least six relatively apolar metabolites. all of these, except ecdysonoic acid, were hydrolysed by a crude enzyme preparation from helix pomatia, releas ... | 1991 | 1944416 |
| performance of pigs with mixed nematode infections before and after ivermectin treatment. | ninety crossbred parasitized pigs were used in two controlled experiments to compare performance before and after ivermectin treatment. four groups of 15 pigs were sequentially infected with 2000 ascaris suum, 10,000 oesophagostomum spp. and 10,000 strongyloides ransomi beginning at average pig weights of either 38 kg (experiment i) or 14 kg (experiment ii). two infected groups of pigs were treated with ivermectin on day 34 (experiment i) or 37 (experiment ii) of the experiments and all pigs wer ... | 1991 | 1957485 |
| effect of dietary zinc on larval burdens, tissue eosinophil numbers, and lysophospholipase activity of ascaris suum infected mice. | the effect of dietary zinc on larval burdens, tissue eosinophil numbers, and lysophospholipase (lpl) activity in the lungs and livers of balb/c mice infected with ascaris suum was evaluated. a suum larval numbers were increased in both liver and lungs in low zinc groups during primary and secondary infection as assessed at days 2 and 7 after egg administration. in the same groups, lpl activity and eosinophil numbers were reduced at both time points and in both tissues, with the exception of lung ... | 1990 | 1973021 |
| structure and expression of ascaris suum collagen genes: a comparison with caenorhabditis elegans. | we are interested in the structure and organization of collagen and collagen genes in the parasitic nematode ascaris suum and in the control of collagen gene expression in ascaris. in this nematode, as in all others studied, collagens constitute the major component of the extracellular cuticle and ultimately we would like to correlate the expression pattern of ascaris cuticular collagen genes with the structure of the nematode cuticle. we would also like to see if there is any correlation betwee ... | 1990 | 1978528 |
| the cuticular biology in developmental stages of ascaris suum. | the cuticles from distinct developmental stages of ascaris suum were isolated by a combination of mechanical disruption and detergent treatment of larvae or by surgical removal of cuticle from adults. proteins from the isolated cuticles were solubilized with sds and 2-mercaptoethanol (2me) and analyzed by page. cuticular proteins from the third and fourth larval stages (l3 and l4) were comparable to adult, but differences in the number of bands were observed. the soluble proteins from the adult, ... | 1990 | 1978529 |
| analysis of the cuticular collagens of ascaris suum. | the nematode cuticle is an extracellular structure composed mainly of collagens, with an insoluble epicuticle on the surface. the extracted collagens from adult ascaris suum can be separated by sds-page into three major groups of polypeptides with apparent molecular masses of 34, 60-70 and 120-140 kda. densitometric evaluation of the polypeptide bands indicated that the three groups are present in the ratio of 1:2:6. rotary shadowing of reduced, extracted molecules showed fibers 45 nm in length. ... | 1990 | 1978530 |
| antibodies against the cuticlin of ascaris suum cross-react with epicuticular structures of filarial parasites. | the insoluble cuticlin from the cortical zone of the cuticle of adult ascaris suum was purified and used to raise antibodies in c57/bl mice. the specificity of the antibodies for the external cortical layer was shown in the indirect immunofluorescence antibody test and by immunoelectronmicroscopy. a very high specificity for the external cortical layer was found. some cross-reactions with cuticular collagens occurred, and increased after booster immunizations. the anti-cuticlin antibody cross-re ... | 1990 | 1978533 |
| kinetic mechanism of nad:malic enzyme from ascaris suum in the direction of reductive carboxylation. | initial velocity studies in the absence and presence of product and dead-end inhibitors suggest a steady-state random mechanism for malic enzyme in the direction of reductive carboxylation of pyruvate. for this quadreactant enzymatic reaction (mn2+ is a pseudoreactant), initial velocity patterns were obtained under conditions in which two substrates were maintained at saturating concentrations while one reactant was varied at several fixed concentrations of the other. data from the resulting rec ... | 1991 | 1993653 |
| antigen-induced phospholipase d activation in rat mast cells is independent of protein kinase c. | in the present study, we investigated the involvement of protein kinase c (pkc) in antigen (ag, dnp-ascaris suum)-induced phospholipase d (pld) activation of rat peritoneal mast cells. phorbor myristate acetate (pma) as well as ag activated pld as inferred by phosphatidylethanol (pet) production. pkc inhibitors, staurosporine and h-7, however, failed to suppress pma-stimulated pld activation, suggesting that pld activation by pma is independent of pkc. by contrast, ag-stimulated pld activity was ... | 1991 | 1998501 |
| effects of single and multiple inhalations of antigen on airway responsiveness in monkeys. | airway hyperresponsiveness is an important and characteristic feature of asthma. in monkeys, we have reported that antigen (ag) inhalation induces a prolonged airway eosinophilia, that chronic airway eosinophilia is associated with marked airway hyperresponsiveness, and that chronic ag inhalations induce airway eosinophilia and hyperresponsiveness. in this study we have determined the effects of acute ag inhalation(s) on airway responsiveness to obtain a protocol for the study of the mechanisms ... | 1991 | 2013678 |
| separation of late bronchial responses from airway hyperresponsiveness in allergic sheep. | allergic sheep with antigen-induced early and late responses were used to determine whether airway hyperresponsiveness (ahr) to carbachol is present during the late response and whether blocking the late response with the leukotriene d4 (ltd4) antagonist mk-571 also blocks this ahr. to do this, we first showed that mk-571 blocked the antigen-induced late response, and then, in a separate study, we determined the effect of mk-571 treatment on airway responsiveness 6 h after antigen challenge (at ... | 1991 | 2022553 |
| modification of a thiol at the active site of the ascaris suum nad-malic enzyme results in changes in the rate-determining steps for oxidative decarboxylation of l-malate. | a thiol group at the malate-binding site of the nad-malic enzyme from ascaris suum has been modified to thiocyanate. the modified enzyme generally exhibits slight increases in knad and ki metal and decreases in vmax as the metal size increases from mg2+ to mn2+ to cd2+, indicative of crowding in the site. the kmalate value increases 10- to 30-fold, suggesting that malate does not bind optimally to the modified enzyme. deuterium isotope effects on v and v/kmalate increase with all three metal ion ... | 1991 | 2043616 |
| characterization of cdna clones for the beta subunit of pyruvate dehydrogenase from ascaris suum. | the pyruvate dehydrogenase complex occupies a unique position in the anaerobic mitochondrial metabolism of the parasitic nematode ascaris suum. this paper describes cdna clones for the beta subunit of the pyruvate dehydrogenase component of this complex. a cdna library has been constructed in lambda gt11 from poly(a)+ rna isolated from adult ascarid body wall muscle. the library was screened with antiserum prepared against the beta subunit of pyruvate dehydrogenase. full-length clones of 1.2 kb ... | 1991 | 2052042 |
| neuropeptides in the nematode ascaris suum. | | 1991 | 2057216 |
| a survey of gastrointestinal parasites in pigs of the plateau and rivers states, nigeria. | faecal samples were collected from a total of 1,000 pigs from the port harcourt and jos areas of the rivers and plateau states, respectively, between january 1987 and march 1988. in the jos area the parasite incidence was: ascaris suum 53.1%, trichuris suis 8.5%, hyostrongylus rubidus 13.1%, metastrongylus salmi 3.7%, strongyloides ransomi 87.7%, oesophagostomum dentatum 35.1% and eimeria spp. 2.4% while in port harcourt the incidence rate was ascaris suum 10.4%, trichuris suis 47.2%, oesophagos ... | 1990 | 2092354 |
| [studies of serum lipids in guinea pigs with larval ascariasis]. | the experiments were carried out on a group of 90 males of guinea pigs of 300 +/- 50 g in weight. the animals were divided into 6 groups equal in numbers. 5 of them were infected per os with ca 5000 invasive eggs of ascaris suum. the blood of both infected and control guinea pigs was controlled on 1, 2, 4, 7, and 20th day after infection. the level of total lipids (according to swahn method), wkt (dole titrimetric method), total cholesterol and hdl cholesterol (błaszczyszyn method) and triglycer ... | 1990 | 2092676 |
| embryonation and infectivity of ascaris suum eggs. a comparison of eggs collected from worm uteri with eggs isolated from pig faeces. | ascaris suum eggs were collected from pig faeces or dissected from worms obtained from the same pigs. eggs from the two sources were allowed to embryonate in 0.1 n h2so4, in 1% buffered formalin or in tap water. the embryonation of the sulphuric acid and water cultures occurred at the same speed, while the formalin cultures developed slightly more slowly. by experimental inoculation of helminth-free pigs and subsequent counting of white spots in the livers and larvae in the lungs day 7 p.i., the ... | 1990 | 2099616 |
| ascaris suum: influence of egg density on in vitro development from embryonated egg to infective stage. | | 1990 | 2099626 |
| further characterization of ascaris suum component(s) with suppressive activity on the ige antibody response. | in order to characterize the component(s) of ascaris suum responsible for damping of the ige antibody production we demonstrated that the extract incubated in sodium acetate buffer, ph 4.5, maintained its suppressive effect and the same protein banding pattern by sds-page. elimination of the lipoprotein components of the extract also left its damping properties unchanged. sds-page of the lipoprotein-free extract revealed practically the same pattern as shown by the whole extract, except for the ... | 1990 | 2101078 |
| divalent hapten-induced intestinal anaphylaxis in the mouse enhances macromolecular uptake from the stomach. | the capacity of the stomach to participate in anaphylaxis induced by the hapten n,n'-di-2,4,dinitrophenyllysine (di-dnp-lysine) was examined in bdf1 female mice immunized with dinitrophenylated ascaris suum extract. immunized animals underwent laparotomy and nontraumatic pyloric occlusion using a microvascular clamp. following wound closure, animals were gavage-fed ovalbumin together with di-dnp-lysine. other mice were subjected to systemic anaphylaxis by intravenous injection of di-dnp-lysine a ... | 1990 | 2107115 |
| mechanisms of decrease in cytoplasmic motility of alveolar macrophages during immediate asthmatic response in dogs. | to study the cytoplasmic motility (cm) of alveolar macrophages (am) during immediate asthmatic response, we measured remanent filed strength (rfs) in dogs in vivo after ascaris suum-antigen (aa) inhalation. four days after instillation of ferrimagnetic particles (fe3o4, 3 mg/kg) into the right lower lobe bronchus, rfs was measured at the body surface immediately after magnetization of the fe3o4 particles by an externally applied magnetic field. rfs decreased with time because of particle rotatio ... | 1990 | 2110421 |
| a bradykinin-antagonist blocks antigen-induced airway hyperresponsiveness and inflammation in sheep. | the effects of a new bradykinin-antagonist, npc-567 (d-arg [hydroxyproline3, d-phenylalanine7] bradykinin) were studied on antigen-induced airway hyperresponsiveness and inflammation in allergic sheep (n = 7). specific lung resistance (srl) was used to assess airway responses to inhaled ascaris suum-antigen. airway responsiveness was determined from slopes of cumulative dose-response-curves (drc) to inhaled carbachol. drcs were performed at baseline and 2 h after an inhalation challenge with ant ... | 1990 | 2135209 |
| relationship of the prevalence of swine helminths to management practices and anthelmintic treatment in danish sow herds. | during 1982-1984, the occurrence of helminths in 66 sow herds in denmark was examined by means of faecal samples. the correlations between the prevalences of ascaris suum and oesophagostomum spp. and management practices were analysed for fatteners and sows, respectively, resulting in four multivariate models. the helminth prevalences were in general low in large herds, specific pathogen-free (spf) herds and herds with low weaning age. the multivariate analyses showed that only the two latter co ... | 1990 | 2144677 |
| catalase activity during the development of the parasitic nematode, ascaris suum. | 1. catalase activity was partially purified from body wall muscle of the parasitic nematode, ascaris suum, and was similar to catalases isolated from mammalian tissues. it exhibited a broad ph optimum and was unaffected by 2 mm ethylenediaminetetra-acetate. in contrast, it was inhibited reversibly by 1 mm cyanide and irreversibly by prior incubation in 40 mm 3-amino-1:2:4-triazole for 1 hr or heating at 80 degrees c for 15 min. 2. catalase activity was highest in the unembryonated "egg" and decr ... | 1990 | 2160878 |
| cellular ltb4 production differs in allergic sheep with and without late airway responses. | we tested the hypothesis that allergic sheep that develop both early and late airway responses to inhaled ascaris suum antigen (late responders) have an increased capacity to generate leukotrienes (lts) compared with allergic sheep that show only early responses to inhaled antigen (acute responders). to test this hypothesis, we measured ltb4 production, in vitro, by granulocytes isolated from peripheral blood and by macrophages isolated from bronchoalveolar lavage (bal) from both groups of sheep ... | 1990 | 2166443 |
| protein kinase activities during early development of ascaris suum. | | 1990 | 2177849 |
| use of monoclonal antibodies for the characterization of onchocerca volvulus antigens. | in order to identify onchocerca volvulus antigens that could be considered as either diagnostic and/or immunoprophylactic, mouse monoclonal antibodies were produced against o. volvulus soluble antigens. three were selected on the basis of their staining patterns in an indirect fluorescent antibody assay carried out on cryosections of adult o. volvulus. the first monoclonal antibody (k1-159) recognized a cuticular antigen which appeared in ifa to be restricted to the genus onchocerca. however, ne ... | 1990 | 2187226 |
| serum levels of gastrin, insulin and glucagon as possible factors of anorexia in pigs infected once with ascaris suum. | in order to determine possible mediators for development of anorexia in pigs infected with ascaris suum, serum levels of gastrin, insulin and glucagon were measured. after a single high oral dose of 100,000-200,000 embryonated eggs the serum levels of gastrin and insulin in the infected pigs did not significantly differ from those in controls. serum glucagon levels in the infected groups, however, were lower than those in controls and the difference was more evident 24 days postinoculation and l ... | 1990 | 2205042 |
| a set of trnas that lack either the t psi c arm or the dihydrouridine arm: towards a minimal trna adaptor. | the mitochondrial dna (mtdna) molecules of the nematode worms, caenorhabditis elegans and ascaris suum contain 22 putative genes for non-standard forms of trnas. the inferred transcripts can be folded into 20 separate structures each resembling a trna whose t psi c arm and variable loop are replaced with a simple loop of 6-12 nucleotides. in two further structures [that resemble trnas for ser(ucn) and ser(agn)], the dihydrouridine arm is replaced by a loop of 5-8 nucleotides. by hybridizing mt-t ... | 1990 | 2209550 |
| primary structure of the major pepsin inhibitor from the intestinal parasitic nematode ascaris suum. | the major pepsin inhibitor from ascaris suum was isolated by affinity chromatography and chromatofocusing. its amino acid sequence was determined by automated edman degradation of peptide fragments. peptides were produced by chemical and enzymatic cleavage of pyridylethylated protein and were purified by reverse-phase high-performance liquid chromatography. the inhibitor consists of 149 residues with the following sequence: qflfsmstgp10fictvkdnqv20fvanlpwtml30egddiqvgke40 faarvedctn50vkhdmaptct6 ... | 1990 | 2223768 |
| late-phase bronchial vascular responses in allergic sheep. | sheep were classified on the basis of their airway response to ascaris suum antigen aerosols as allergic or nonsensitive. allergic sheep were classed as acute or dual responders. acute responders had only an immediate increase in mean airflow resistance after antigen, whereas dual responders had an immediate and late-phase (6-8 h after antigen challenge) increase in mean airflow resistance; nonsensitive sheep had minimal airway responses to antigen (less than 30% increase from base line). the sh ... | 1990 | 2228869 |
| evidence for the frequent use of ttg as the translation initiation codon of mitochondrial protein genes in the nematodes, ascaris suum and caenorhabditis elegans. | data obtained from alignments of nucleotide sequences of mitochondrial (mt) dna molecules of the nematode worms ascaris suum and caenorhabditis elegans indicate that in six of the mt-protein genes of a. suum and three of the mt-protein genes of c. elegans ttg is used as the translation initiation codon. also, gtt seems to be the translation initiation codon of the a. suum coiii gene. all of the five remaining a. suum mt-protein genes appear to begin with att and the remaining nine c. elegans mt- ... | 1990 | 2235493 |
| effects of multiple dose infections with ascaris suum on blood gastrointestinal hormone levels in pigs. | ten consecutive daily doses of infective ascaris suum eggs were administered to pigs in two experiments and the levels of gastrointestinal hormones in their blood were measured. the piglets in each experiment were divided into low-dose (ldi) and high-dose (hdi) infections and control groups. infected pigs had lower feed consumption, lower weight gains, and lower feed efficiency than control pigs. serum gastrin levels in infected pigs were significantly lower than the controls from days 7 to 17 p ... | 1990 | 2238428 |
| [trypsin and alpha-amylase activities in the pancreas of guinea pigs. ii. effect of per os and intra-intestinal infection with invasive ova of ascaris suum]. | the guinea pigs were infected orally or intraintestinally (after laparotomy) with 5000 invasive eggs of ascaris suum. on the 7th day after infection the invasion was controlled by lung's weight, degree of congestion, and number of larvae in the lungs. the activity of amylase was determined according to fennel method, trypsin to anson method. the activities of both enzymes in pancreas of orally infected animals (after laparotomy or not) were lower, and relative weight of lungs of these animals wa ... | 1990 | 2256335 |
| [trypsin and alpha-amylase activities in the pancreas of guinea pigs. iii. effect of homogenates of invasive ova of ascaris suum and organic acids c2-c6 on the activity of enzymes in pancreatic extracts]. | the studies were carried out on pancreas extracts from guinea pigs free of parasite invasion. the activity of trypsin was determined according to the method of anson, and amylase of fennel method. the measurements of activity were done at the presence of homogenized invasive eggs of ascaris suum, and the mixture of volatile organic acids c2-c6 (at concentrations of 30.3 and 0.3 mm/l). in samples with homogenized invasive eggs of a. suum the amylase activity remained unchanged while the activity ... | 1990 | 2256339 |
| [helminth infections in wild boars in enclosures in southern germany: species spectrum and infection frequency]. | to determine the helminth fauna of wild boars, stomachs and intestines of 124, lungs of 62, and livers of 39 animals from 5 enclosures in southern germany were examined. the evaluation of the species composition revealed at least 13 species: metastrongylus apri, m. salmi, m. pudendotectus, physocephalus sexalatus, hyostrongylus rubidus, globocephalus longemucronatus, ascaris suum, trichuris suis, oesophagostomum dentatum, o. quadrispinulatum, fasciola hepatica, c. tenuicollis and capillaria. the ... | 1990 | 2264058 |
| molecular cloning and expression of an immunodominant 53-kda excretory-secretory antigen from trichinella spiralis muscle larvae. | a trichinella spiralis cdna expression library was constructed in lambda gt11 from muscle larvae mrna and immunologically screened to identify genes encoding previously described immunodiagnostic excretory-secretory (es) antigens. screening the library with t. spiralis infection serum from swine or rabbit antiserum to t. spiralis es antigen identified one clone, designated tsa-12, that contains a cdna transcript 539 bp in length and codes for an apparent 123-kda beta-galactosidase fusion protein ... | 1990 | 2270099 |
| effect of nonadrenergic noncholinergic inhibitory nerve stimulation on the allergic reaction in cat airways. | to examine the effect of nonadrenergic noncholinergic (nanc) inhibitory nerve stimulation on the antigen inhalation with allergic animals, changes in pulmonary resistance (rl) and arterial plasma histamine concentration ([h]) caused by inhalation of ascaris suum antigen were studied in five control (group a) and five nerve-stimulated (group b) cats, which were anesthetized and mechanically ventilated. all animals were actively sensitized with ascaris antigen before the experiment. after choliner ... | 1990 | 2297183 |
| acute topical steroid administration blocks mast cell increase and the late asthmatic response of the canine peripheral airways. | previous studies indicated that mast cell number increased after airway exposure to ascaris suum antigen (ag). the following two series of experiments were performed to test the hypothesis that this phenomenon may be associated with the ag-induced late-phase bronchoconstriction (lar). in the first, two bronchoscopes wedged in airway segments of two contralateral lobes of 16 dogs were used to deliver 0.26, 2.6, or 26 micrograms of ag to one lobe; the other served as a control. after the observati ... | 1990 | 2301857 |
| penetrating the conjunctival barrier. the role of molecular weight. | dinitrophenyl (dnp) derivatives of various molecular weights were tested for their ability to elicit ocular anaphylaxis after topical application to the eye of immunized animals. adult male sprague-dawley rats were immunized by intraperitoneal injection of dnp-ascaris suum extracts and alum and were then skin-tested with dnp-bovine serum albumin on day 13 post-immunization to assess their sensitivity to the dnp hapten. on day 14, animals were challenged topically with dnp derivatives in one eye; ... | 1990 | 2303328 |
| capsaicin and histamine antagonist-sensitive mechanisms in the immediate allergic reaction of pig airways. | the airway vascular and bronchial responses were studied in pigs sensitized with ascaris suum. ascaris, histamine (h) and capsaicin aerosol all induced a clear-cut increase in blood flow in the nasal, laryngeal and bronchial circulation with a decrease in vascular resistance of 20-40%. when delivered to the lung both ascaris and histamine, but not capsaicin, caused pulmonary airflow obstruction with increase in resistance and a fall in dynamic compliance of 40-70%. after pretreatment of pigs wit ... | 1990 | 2309568 |
| inhibitory effect of renal nerve activity during canine anaphylactic hypotension. | we have examined baroreceptor regulation of renal nerve activity (rna) during anaphylactic hypotension that affects renal nerve activity. in anesthetized dogs, mean blood pressure (mbp), heart rate (hr), and rna were simultaneously measured. to test for a normally functioning baroreceptor reflex in each animal, a transient hypotension was induced by an intravenous injection of sodium nitroprusside (4 micrograms/kg). nitroprusside produced a reflex increase in rna (+63 +/- 12% at -20 mmhg and +13 ... | 1990 | 2309932 |
| in vitro induction of crawling in the amoeboid sperm of the nematode parasite, ascaris suum. | in a highly synchronous process, the immotile spermatids of ascaris suum extend pseudopods and become rapidly crawling sperm when treated with an extract from the glandular vas deferens of the male under strict anaerobic conditions. within 9-12 min, a pseudopod develops, elongates rapidly, and exhibits a continuous flow of membrane specializations, the villipodia, from tip toward base. when attached to acid-washed glass, the pseudopod pulls the cell body along at speeds exceeding 70 microns/min. ... | 1990 | 2311127 |
| evaluation of pyrantel-tartrate abbreviated ascaris suum infections for the development of resistance in young pigs against migrating larvae. | crossbred young pigs were used to test whether abbreviated infections with eggs of ascaris suum can stimulate the acquisition of resistance to challenge. weanling pigs from an ascaris-free colony were kept free of a. suum until they were divided into groups at the age of 7-8 weeks. the experimental animals received pyrantel tartrate during the period when they were being exposed to increasing numbers of infective a. suum eggs and challenged 10 days after the last infective dose. liver milk-spot ... | 1990 | 2312230 |
| putrescine n-acetyltransferase in onchocerca volvulus and ascaris suum, an enzyme which is involved in polyamine degradation and release of n-acetylputrescine. | a novel type of n-acetyltransferase, clearly different from the nuclear and cytosolic polyamine n-acetyltransferases of mammals, was recently found in the intestinal nematode ascaris suum. the occurrence of this putrescine n-acetylating enzyme has also been noted in the filarial parasite onchocerca volvulus. the enzyme was partially purified from adults of o. volvulus and a. suum by chromatography on deae-cellulose and cadaverine-sepharose. substrate specificities of the filarial enzyme resemble ... | 1990 | 2320051 |
| structural studies on three flavin-interacting regions of the flavoprotein subunit of complex ii in ascaris suum mitochondria. | the flavoprotein (fp) subunit of mitochondrial complex ii contains covalently bound fad as a prosthetic group. in this study, the primary structure of the flavin-bound tryptic peptide from the fp subunit of ascaris complex ii was determined and found to be highly similar to those of the corresponding flavin-binding regions of bovine heart and bacterial fp subunits. furthermore, the ascaris fp subunit was shown to contain two regions exhibiting striking sequence similarity to the segments that ha ... | 1990 | 2335235 |
| n-terminal amino acid sequence identity between a major allergen of ascaris lumbricoides and ascaris suum, and mhc-restricted ige responses to it. | a protein allergen of the parasitic nematode ascaris has been purified to homogeneity by immunoaffinity chromatography. it is the most abundant protein species in the parasite's body fluid and has been named aba-1. the allergen's molecular weight (mw) has been previously estimated at 14,000, but this sizing is currently under re-evaluation. the immunological activity of the protein was intact after purification, as attested by immunoprecipitation and passive cutaneous anaphylaxis. the ige respon ... | 1990 | 2335378 |
| experimental pulmonary eosinophilia in mice by ascaris suum extract. | the transnasal administration of an extract of the parasite ascaris suum (asc) to c57bl/6 mice for 3 wk produced marked eosinophilia in the bronchoalveolar lavage (bal) fluid. this pulmonary eosinophilia was not accompanied by blood eosinophilia. the oral administration of cyclosporin, 50 mg/kg body weight, every other day significantly suppressed the pulmonary eosinophilia. athymic c57bl/6-nu/nu mice failed to develop pulmonary eosinophilia. these data indicate that pulmonary eosinophilia cause ... | 1990 | 2339848 |
| acquired resistance to migrating larvae of ascaris suum in young pigs by repeated drug-abbreviated infections. | cross-bred 3- and 8-wk-old pigs were used to test whether drug-abbreviated infections with ascaris suum can stimulate acquired resistance to challenge. during the immunization period, both age groups of animals were infected with increasing numbers of a. suum eggs (500, 1,000, 2,000, 5,000, 10,000, and 20,000) at 7-day intervals while the pigs were receiving pyrantel tartrate in the feed. two days after the last infective dose, animals were placed on unmedicated feed for 8 days and then challeng ... | 1990 | 2352069 |
| the specificity of the antibody response to internal antigens of ascaris: heterogeneity in infected humans, and mhc (h-2) control of the repertoire in mice. | children from an area of africa endemic for the large roundworm of humans, ascaris lumbricoides, were found to vary considerably in the specificity of their serum igg response to the internal antigens of the parasite. this was particularly noticeable for responses to a 14-kd protein (aba-1) of the parasite that has previously been shown to be the subject of a strong ige antibody response in infected animals. the possibility that this heterogeneity in immune repertoire has a genetic basis was exp ... | 1990 | 2357848 |
| elisa detection of o. volvulus antigenemia in snip-positive patients by monoclonal antibodies directed against a 56 kd glycoprotein of ascaris suum. | | 1990 | 2378214 |
| tumor necrosis factor-like cytotoxicity and anorexia in ascaris suum infected pigs. | tumor necrosis factor-like cytotoxicity (tnf-lc) was examined in sera from 12 pigs experimentally infected with ascaris suum. the difference of tnf-lc levels between eight infected and four uninfected controls was not significant. when an endotoxin challenge was intravenously administered 1 month after the first dose of a. suum, the levels of tnf-lc in the sera of infected pigs were one-third that of the controls 125 min post-challenge (pc). in a more detailed study on four infected and two unin ... | 1990 | 2382388 |
| ozone increases susceptibility to antigen inhalation in allergic dogs. | to determine whether o3 exposure increased airway responsiveness to antigen inhalation, we studied airway responsiveness to acetylcholine (ach) and ascaris suum antigen (aa) before and after o3 in dogs both sensitive and insensitive to aa. airway responsiveness was assessed by determining the provocative concentration of ach and aa aerosols that increased respiratory resistance (rrs) to twice the base-line value. o3 (3 parts per million) increased airway responsiveness to ach in dogs both sensit ... | 1990 | 2384406 |
| biotin as a probe of the surface of ascaris suum developmental stages. | sulfo-nhs-biotin (aqueous soluble) and nhs-biotin (organic soluble) labeled similar sds-2me (sodium dodecyl sulfate/beta-mercaptoethanol) soluble cuticular proteins of second stage larvae (l2) and third stage ascaris suum larvae (l3). comparable analysis of biotin-labeled fourth stage larvae (l4), young adults, and mature adult ascaris suum revealed strong labeling of several sds-2me soluble cuticular proteins with nhs-biotin, while sulfo-nhs-biotin appeared to strongly label a single sds-2me so ... | 1990 | 2385267 |
| [analysis of antigens defined by anti-anisakis larvae antibodies of ige and igg type in the sera of patients with acute gastrointestinal anisakiasis]. | using sds-page and immunoblotting assay, we analysed antigens defined by anti-anisakis larvae antibodies of ige and igg type in the sera of patients with acute gastrointestinal anisakiasis. 1) humoral ige response to 76, 64 and 60 kda molecular weight proteins of anisakis larvae and igg response to 91, 76, 64 and 60 kda molecular weight proteins were demonstrated in the sera of patients with acute gastric anisakiasis. those humoral antibody responses were also demonstrated in the sera of two pat ... | 1990 | 2388423 |
| biophysical transport properties of the cuticle of ascaris suum. | the transport properties of isolated cuticle from ascaris suum were studied using standard two-chamber diffusion cells and a number of radiolabeled permeants which varied in molecular size, lipophilicity and electrical charge. the permeability coefficient of the collagen matrix (lipid-extracted cuticle) vs. molecular radius relationship showed the interdependence of molecular size and electrical charge of the permeants with respect to the aqueous pores of the negatively charged matrix. the perme ... | 1990 | 2398915 |
| sympathetic modulation of biochemical and physiological response to immune degranulation in canine bronchial airways in vivo. | the effect of sympathetic stimulation on bronchial smooth muscle contractile response after mast cell degranulation with ascaris suum antigen was studied in 36 natively allergic dogs in situ. bronchial smooth muscle response was measured isometrically in a single right middle lobe bronchus. a dose of antigen causing maximal release of mediator was administered to the bronchus through the bronchial arterial circulation. serial plasma histamine concentrations were determined at 15-s intervals afte ... | 1985 | 2409114 |
| augmentation of respiratory mast cell secretion of histamine caused by vagus nerve stimulation during antigen challenge. | we studied the effect of vagus nerve stimulation on the mast cell secretion of histamine after intraarterial (i.a.) administration of ascaris suum antigen (aa) into the bronchial circulation of 10 randomly selected, natively allergic dogs in vivo. respiratory mast cell response was measured as the arteriovenous difference (avd) in histamine concentration across the bronchus. plasma histamine concentration was determined simultaneously from right atrium, right ventricle, and femoral artery 60 and ... | 1986 | 2416826 |
| isolation of specific antigens from angiostrongylus cantonensis. 1. preparative flatbed isoelectric focusing. | electrophoresis on sds gel and analytical isoelectric focusing showed that a crude extract of angiostrongylus cantonensis consisted of at least 40 protein components with molecular weights ranging from 13 000-70 000 and isoelectric points of pi values ranging from 3.7-10.0. crossed-immunoelectrophoresis with a hyperimmune antiserum to a. cantonensis showed at least 40 different antigenic components in the crude worm extract which were cross-reactive with those of ascaris suum, metastrongylus apr ... | 1985 | 2417425 |
| purification of ascaris suum antigen: its allergenic activity in vitro and in vivo. | crude aqueous extracts of ascaris suum (ce) have been used widely to study ige-mediated reactions in various experimental preparations. because some ce may contain a polypeptide, a mast cell degranulating peptide (mcdp), that degranulates mast cells by nonimmunologic mechanisms, various protocols have been used to ensure that the ascaris preparation used did not contain mcpd. in general, these protocols have assumed mcdp had been without providing proof. even protocols designed to isolate the ma ... | 1986 | 2419382 |
| increased metachromatic cells and lymphocytes in bronchoalveolar lavage fluid of dogs with airway hyperreactivity. | bronchoalveolar lavage (bal) was performed in 11 basenji greyhound (bg) dogs, which showed persistent airway hyperreactivity to methacholine and citric acid aerosols, and in 15 non-bg dogs, which were significantly less reactive to these challenges. five of the bg dogs had never received any aerosols prior to bal, and 3 of the non-bg dogs were allergic to ascaris suum. no dog received aerosols for 2 wk prior to bal. fluid recovered was centrifuged, and aliquots were taken for histamine content a ... | 1986 | 2420243 |
| studies on bronchial hyperreactivity, allergic responsiveness, and asthma in rural and urban children of the highlands of papua new guinea. | the prevalence of asthma and allergic responsiveness in rural and urban children of the highlands of papua new guinea was studied. bronchial provocation studies with histamine demonstrated significant bronchial hyperreactivity in 0.5% (1 in 195) rural and 1.7% (1 in 59) urban children, rates which were significantly lower than those observed in corresponding adult populations (7%). urban children demonstrated a higher incidence of skin test reactivity toward dermatophagoides pteronyssinus, asper ... | 1986 | 2420852 |
| blocking of nonspecific igg in indirect radioimmunoassay for detecting ascaris suum antigens. | the sensitivity of an indirect radioimmunoassay (iria) used for detecting larval body wall (lbw) antigens of ascaris suum was enhanced by using various blocking agents which prevented nonspecific binding of immunoglobulin g (igg) or free 125i without preventing binding of specific antibodies to the antigen. the use of blocking agents reduced counts for both positive and negative sera, resulting in an increase in calculated binding ratios (br) and deltas and, thus, in the sensitivity of the assay ... | 1986 | 2424166 |
| exploratory and confirmatory plots of single-channel records. | graphical displays are used to study open- and closed-time durations of a single ion-channel. cusum plots, in conjunction with kolmogorov-smirnov type tests, provide checks for changes in channel behaviour. distributional properties are examined by means of q-q and p-p plots. the methods are illustrated using gaba-activated channel records from ascaris suum. | 1986 | 2425195 |
| the pharmacology of nedocromil sodium. | the pathophysiology of obstructive airways disease involves, in varying degree, components of reversible bronchoconstriction, inflammation and bronchial hyperreactivity. this multifactorial aetiology is the probable reason why no single animal model exists which is predictive of therapeutic efficacy in airway diseases, including asthma. nedocromil sodium has therefore been profiled in a number of systems ranging from simple in vitro tests and in vivo models of passive anaphylaxis to complex mode ... | 1986 | 2429854 |
| central role of cyclooxygenase in the response of canine peripheral airways to antigen. | we studied the effects of antigen aerosol challenge on the airways of the canine peripheral lung and examined the roles of cyclooxygenase products, histamine, and cholinergic activity in the responses. one-minute deliveries of 1:10,000 or 1:100,000 concentrations of ascaris suum antigen aerosol through a wedged bronchoscope resulted in mean maximal increases in collateral system resistance (rcs) of 415 and 177%, respectively, after 4-8 min. repeated antigen challenge (1:100,000) resulted in sign ... | 1986 | 2430928 |
| characterization of primate bronchoalveolar mast cells. i. ige-dependent release of histamine, leukotrienes, and prostaglandins. | large numbers of functional mast cells were obtained by bronchoalveolar lavage (bal) of macaca arctoides monkeys that had been infected with the nematode ascaris suum. these lavage cells, of which 21% were mast cells, released histamine, ltc4, and pgd2 in a concentration-dependent fashion when challenged with ascaris antigen or antibody to human ige. however, there was no release of histamine when these cells were challenged with compound 48/80. the amount of mediator released was highly depende ... | 1986 | 2431048 |
| characterization of primate bronchoalveolar mast cells. ii. inhibition of histamine, ltc4, and pgd2 release from primate bronchoalveolar mast cells and a comparison with rat peritoneal mast cells. | as described in the preceding companion paper, bronchoalveolar lavage (bal) of the primate macaca arctoides infected with the nematode ascaris suum yields a population of cells containing a high proportion of mast cells (21%). nedocromil sodium, a new drug undergoing clinical evaluation for the treatment of reversible obstructive airways disease, inhibited the release of histamine, ltc4, and pgd2 from these cells challenged with antigen (with ic30 values of 2.1 x 10(-6) m, 2.3 x 10(-6) m, and 1. ... | 1986 | 2431049 |
| effects of the new antiallergic drug 11-oxo-11h-pyrido[2,1-b] quinazoline-2-carboxylic acid on bronchial and cutaneous allergic responses to ascaris in dogs. | the effects of 11-oxo-11h-pyrido[2,1-b]quinazoline-2-carboxylic acid (sm 857), a new antiallergic drug, on both bronchial asthma and active cutaneous anaphylaxis (aca) reaction induced by ascaris suum antigen in dogs were investigated. the airway resistance was determined using the modified konzett-rössler method. sm 857 in doses of 30 and 100 mg/kg intraduodenally (i.d.) produced a remarkable inhibitory effect on the asthmatic bronchoconstriction induced by inhalation of ascaris antigen in natu ... | 1986 | 2434112 |
| antigenic similarity of onchocerca volvulus to other helminths examined by monoclonal antibodies against o. volvulus. | antigens in onchocerca volvulus were characterized using anti-o. vovulus monoclonal antibodies (mcab). a total of 17 mcab which were not species-specific to o. volvulus was examined for the antigenic similarity of o. volvulus with 12 parasitic helminths using indirect immunofluorescence. the range of reactivity of these mcab was wider in nematodes in contrast to species of cestoda or trematoda. the antigenic similarity within helminths examined was generally in agreement with their taxonomic rel ... | 1986 | 2434696 |
| a high-conductance calcium-dependent chloride channel in ascaris suum muscle. | the properties of a high-conductance ca-dependent cl channel in the body muscle membrane of the nematode parasite ascaris suum were examined with the patch-clamp technique. in ascaris ringer solution the current-voltage relationships of the channel appeared linear during hyperpolarization with a slope conductance of 200 ps (in symmetrical 175 mm-cl): during depolarization the slope conductance reduced. cation and anion replacement experiments showed this channel to be permeable to cl ions. in ar ... | 1987 | 2436251 |
| the effect of trans-4-guanidinomethylcyclohexanecarboxylic acid p-tert-butylphenyl ester hydrochloride (nco-650) on ascaris suum antigen-induced bronchoconstriction in dogs. | antiallergic asthma effect of trans-4-guanidinomethylcyclohexane-carboxylic acid p-tert-butylphenyl ester hydrochloride (nco-650), a new anti-allergic drug, was investigated in comparison with those of tranilast and disodium cromoglycate (dscg) in anesthetized dogs. the asthmatic bronchoconstriction was induced by inhalation of ascaris suum antigen (asc-ag) to naturally ascaris-sensitive dogs. the airway resistance was determined using the modified konzett-rössler method. both intravenous (1 and ... | 1987 | 2437348 |
| the effects of chlorpheniramine and antiallergic drugs on ascaris suum antigen-induced active cutaneous anaphylaxis in dogs. | effects of chlorpheniramine and two antiallergic drugs on the active cutaneous anaphylaxis (aca) reaction induced by intradermal injection of ascaris suum antigen in naturally sensitized dogs were investigated. chlorpheniramine (10 mg/kg, intraduodenally (i.d.)) almost abolished the aca reaction. nco-650 (100 mg/kg, i.d.) had no inhibitory effect, while tranilast (300 mg/kg, i.d.) showed a weak inhibitory effect. these findings show that the aca reaction is almost totally mediated by histamine a ... | 1987 | 2442443 |
| phosphofructokinase from dirofilaria immitis. stimulation of activity by phosphorylation with cyclic amp-dependent protein kinase. | phosphofructokinase has been partially purified from the filariid helminth, dirofilaria immitis, using ion exchange and affinity chromatography. the d. immitis phosphofructokinase cross-reacted with antibodies prepared against the phosphofructokinase from ascaris suum. these antibodies had been bound to agarose beads. the enzyme was eluted from the immobilized antigen-antibody complex by denaturing agents, and the subunit molecular weight determined by sodium dodecyl sulfate gel electrophoresis ... | 1988 | 2449442 |
| effects of antihistamines on the cardiopulmonary changes due to canine anaphylaxis. | histamine has long been considered to be an important chemical mediator in the pathogenesis of immediate hypersensitivity reactions. we evaluated the efficacy of antihistamines to determine the physiological role of histamine in canine anaphylaxis. either a saline vehicle (control group), an h1 antihistamine (chlorpheniramine, 10 mg/kg), or this h1 antihistamine and an h2 antihistamine (cimetidine, 30 mg/kg) was administered to three separate groups of anesthetized dogs (n = 8). cardiopulmonary ... | 1988 | 2451656 |
| evidence for the absorption and synthesis of 5-hydroxytryptamine in perfused muscle and intestinal tissue and whole worms of adult ascaris suum. | the metabolites of 5-hydroxytryptamine (5-ht, serotonin) namely, l-tryptophan, 5-hydroxytryptophan, 5-hydroxyindole acetic acid and 5-hydroxytryptophol were measured in perfused tissue and whole worms from adult female ascaris suum using reversed-phase liquid chromatography with electrochemical detection. the intracellular levels of each metabolite were quantitated in response to several physiological effectors but only l-tryptophan (trp) caused dose-dependent changes in these metabolites. serot ... | 1988 | 2452401 |
| predictive structure-activity relationships in a series of pyranoquinoline derivatives. a new primate model for the identification of antiallergic activity. | a new primate model has been developed for the evaluation of antiallergic agents. compounds are tested for their ability to inhibit anti-ige induced histamine release from the bronchoalveolar mast cells lavaged from the lungs of macaca arctoides infected with the parasite ascaris suum. a number of 6-substituted pyranoquinoline derivatives have been evaluated and the activities were subjected to hansch analysis. a highly significant correlation with lipophilicity (pi) and hammett sigma p values w ... | 1988 | 2455052 |
| in vitro synthesis and processing of components of the ascaris suum pyruvate dehydrogenase complex. | poly(a)+ rna was isolated from ascaris suum body wall muscle and translated in a cell-free rabbit reticulocyte lysate system. specific antisera and immunoglobulins against the alpha-pyruvate dehydrogenase and dihydrolipoyl transacetylase components of ascarid pyruvate dehydrogenase complex were used to immunoprecipitate individual radiolabelled polypeptides from the in vitro translation mixtures. both polypeptides appeared to be synthesized as preproteins about 1.5 and 8 kda larger than the corr ... | 1988 | 2455226 |
| [histochemical studies of the ova of ascaris suum during embryogenesis. i. phosphatases]. | | 1987 | 2457282 |
| [histochemical studies of the ova of ascaris suum during embryogenesis. ii. lipids and respiratory enzymes]. | | 1987 | 2457283 |
| characterization of isolated porcine intestinal mucosal mast cells following infection with ascaris suum. | porcine intestinal mucosal mast cells (immc) were isolated from intestinal tissues of swine by enzymatic digestion and density gradient separation. helminth-free swine and swine exposed to the nematode parasite, ascaris suum, were used as a source of intestinal tissue. up to 40% of the isolated intestinal cells stained metachromatically with toluidine blue ph 3.0, indicating the presence of immc. the histamine content of this cell population ranged from 2.9-8.9 pg per toluidine blue-positive imm ... | 1988 | 2462304 |
| effect of beta-adrenergic blockade and sympathetic stimulation on canine bronchial mast cell response to immune degranulation in vivo. | we examined the effect of beta-adrenoceptor blockade and residual alpha-adrenoceptor effects during sympathetic stimulation on mast cell secretion of histamine in 12 natively allergic mongrel dogs. bronchial mast cell response was measured as the arteriovenous difference (avd) in plasma histamine concentration [h] across the bronchus. plasma [h] was determined simultaneously from the azygos outflow tract and femoral artery as a marker of mast cell response prior to and for 90 s after intra-arter ... | 1989 | 2463779 |
| ascaris suum ova-induced bronchoconstriction, eosinophilia, and ige antibody responses in experimentally infected primates did not lead to histamine hyperreactivity. | the hypothesis that active immunization of primates to give airway allergic responses would also confer on them a hyperreactivity to a nonspecific stimulus such as histamine was tested in 29 normal rhesus primates. at 6 wk after immunization, specific primate ige (rast) to ascaris antigen had increased from 0.35 +/- 0.17 to 0.98 +/- 0.35 units/ml x 10(2) (p less than 0.05). histamine released from bronchial alveolar lavage cells in response to antigen increased from 5.4 +/- 0.67 to 24 +/- 1.8ng/ ... | 1989 | 2466426 |
| leukotriene c4 and b4 in bronchoalveolar lavage fluid during biphasic allergic bronchoconstriction in sheep. | to study the contribution of leukotriene c4 (ltc4), leukotriene d4 (ltd4), leukotriene b4 (ltb4), and inflammation to the antigen-induced late bronchial response, these leukotrienes, histamine, and cell differentials were evaluated in bronchoalveolar lavage fluid (balf). immediate and late responses were induced by ascaris suum antigen inhalation in 11 conscious sheep. bronchoalveolar lavage was performed on 3 separate test days, before challenge (control), during the immediate response, and dur ... | 1989 | 2466428 |
| antigenic relationships between the surface-exposed, secreted and somatic materials of the nematode parasites ascaris lumbricoides, ascaris suum, and toxocara canis. | the cosmopolitan nematode parasites ascaris lumbricoides, ascaris suum, and toxocara canis are closely related phylogenetically, and are all pathogenic to man. in the case of the latter, the antigens released by the tissue-invasive parasitic larvae in vitro ('excretory/secretory' or 'es' antigens) are routinely used for serodiagnostic purposes. here we have found, using radioimmunoprecipitation with defined rabbit antiserum, and sds-page, that there is a significant antigenic similarity between ... | 1989 | 2467777 |
| chronological relationships between mediator release and changes in airway dynamics during an ascaris response in sensitive cynomolgus monkeys. | recently identified ascaris suum sensitive cynomolgus monkeys were further characterized to determine if a chronologic relationship existed between mediator release and onset of bronchoconstriction. in these anesthetized ascaris-sensitive monkeys, aerosol antigen challenge of each animal produced rapid and severe bronchoconstriction, as determined by decreases in dynamic lung compliance (-80.2 +/- 4.1%) and airway conductance (-64.5 +/- 13.8%). maximum changes were achieved within 5 min followin ... | 1989 | 2470112 |
| [histopathologic and cytochemical studies of the kidneys of guinea pigs infected with larvae of ascaris suum goeze, 1782]. | the material consisted of 20 male guinea pigs of body weight 300 +/- 50 g. fifteen of them were infected per os with a dose of 5000 invasive eggs of a. suum. at the 4th, 7th and 20th days of infection the animals were dissected and sections of kidney were taken for histopathological and histochemical studies. the sections were stained with hematoxylin and eosin. the presence of mucopolisachcarides was detected with the pas method, the activity of acid and alkaline phosphatases--with gomori's met ... | 1989 | 2470207 |
| a patch-clamp study of effects of dihydroavermectin on ascaris muscle. | 1. effects of bath-application of the anti-parasitic agent 22,23-dihydroavermectin b1a (dhavm 20 fm to 0.2 microm) on outside-out and inside-out patches from collagenase-treated muscle membranes of ascaris suum were examined. 2. dhavm was tested as a gamma-aminobutyric acid (gaba) agonist by application to outside-out patches. dhavm failed to open characteristic gaba channels even when high concentrations (20 nm to 0.2 microm) were used. these high concentrations of dhavm also failed to potentia ... | 1989 | 2480169 |
| comparison of collagen gene sequences in ascaris suum and caenorhabditis elegans. | the nucleotide sequences of a 3060-bp fragment containing almost the entire coding sequence of one ascaris suum collagen gene, and a 3019-bp pair fragment containing the 3' end of another a. suum collagen gene have been determined. the polypeptides encoded by these genes show a striking similarity to two caenorhabditis elegans cuticular collagens, both in the position of the triple-helical regions and in the position of cysteine residues. the results of northern blot hybridisation experiments to ... | 1989 | 2482444 |
| effects of the chitin synthesis inhibitor diflubenzuron on development of ascaris suum and haemonchus contortus. | the potential of the chitin synthesis inhibitor diflubenzuron (dfb) to alter the development of the parasitic nematodes (ascaris suum and haemonchus contortus was investigated. dfb given orally (10 mg kg-1 per day for 30 days) to sheep inoculated with h. contortus infective larvae did not prevent the establishment of adults or affect fecal egg output. however, there was a significant (greater than 90%) decrease in the number of infective larvae recovered from fecal cultures derived from lambs ha ... | 1989 | 2505433 |
| glycogen synthesis in the obliquely striated muscle of ascaris suum. | a glycogen synthase, designated gs ii, which occurs in a protein/carbohydrate complex has been purified from ascaris suum muscle. the purified gs-ii complex which is eluted from concanavalin-a--sepharose contains proteins with mr 140,000 and 66,000 and a glycoprotein with a carbohydrate/protein mass ratio of 3:1. gs ii activity was totally dependent on glucose 6-phosphate, but exogenous glycogen was not required for polysaccharide synthesis. the gs-ii complex was not phosphorylated by cyclic-amp ... | 1989 | 2506012 |