Publications

cellular ltb4 production differs in allergic sheep with and without late airway responses.	we tested the hypothesis that allergic sheep that develop both early and late airway responses to inhaled ascaris suum antigen (late responders) have an increased capacity to generate leukotrienes (lts) compared with allergic sheep that show only early responses to inhaled antigen (acute responders). to test this hypothesis, we measured ltb4 production, in vitro, by granulocytes isolated from peripheral blood and by macrophages isolated from bronchoalveolar lavage (bal) from both groups of sheep ...	1990	2166443
protein kinase activities during early development of ascaris suum.		1990	2177849
use of monoclonal antibodies for the characterization of onchocerca volvulus antigens.	in order to identify onchocerca volvulus antigens that could be considered as either diagnostic and/or immunoprophylactic, mouse monoclonal antibodies were produced against o. volvulus soluble antigens. three were selected on the basis of their staining patterns in an indirect fluorescent antibody assay carried out on cryosections of adult o. volvulus. the first monoclonal antibody (k1-159) recognized a cuticular antigen which appeared in ifa to be restricted to the genus onchocerca. however, ne ...	1990	2187226
serum levels of gastrin, insulin and glucagon as possible factors of anorexia in pigs infected once with ascaris suum.	in order to determine possible mediators for development of anorexia in pigs infected with ascaris suum, serum levels of gastrin, insulin and glucagon were measured. after a single high oral dose of 100,000-200,000 embryonated eggs the serum levels of gastrin and insulin in the infected pigs did not significantly differ from those in controls. serum glucagon levels in the infected groups, however, were lower than those in controls and the difference was more evident 24 days postinoculation and l ...	1990	2205042
a set of trnas that lack either the t psi c arm or the dihydrouridine arm: towards a minimal trna adaptor.	the mitochondrial dna (mtdna) molecules of the nematode worms, caenorhabditis elegans and ascaris suum contain 22 putative genes for non-standard forms of trnas. the inferred transcripts can be folded into 20 separate structures each resembling a trna whose t psi c arm and variable loop are replaced with a simple loop of 6-12 nucleotides. in two further structures [that resemble trnas for ser(ucn) and ser(agn)], the dihydrouridine arm is replaced by a loop of 5-8 nucleotides. by hybridizing mt-t ...	1990	2209550
primary structure of the major pepsin inhibitor from the intestinal parasitic nematode ascaris suum.	the major pepsin inhibitor from ascaris suum was isolated by affinity chromatography and chromatofocusing. its amino acid sequence was determined by automated edman degradation of peptide fragments. peptides were produced by chemical and enzymatic cleavage of pyridylethylated protein and were purified by reverse-phase high-performance liquid chromatography. the inhibitor consists of 149 residues with the following sequence: qflfsmstgp10fictvkdnqv20fvanlpwtml30egddiqvgke40 faarvedctn50vkhdmaptct6 ...	1990	2223768
late-phase bronchial vascular responses in allergic sheep.	sheep were classified on the basis of their airway response to ascaris suum antigen aerosols as allergic or nonsensitive. allergic sheep were classed as acute or dual responders. acute responders had only an immediate increase in mean airflow resistance after antigen, whereas dual responders had an immediate and late-phase (6-8 h after antigen challenge) increase in mean airflow resistance; nonsensitive sheep had minimal airway responses to antigen (less than 30% increase from base line). the sh ...	1990	2228869
evidence for the frequent use of ttg as the translation initiation codon of mitochondrial protein genes in the nematodes, ascaris suum and caenorhabditis elegans.	data obtained from alignments of nucleotide sequences of mitochondrial (mt) dna molecules of the nematode worms ascaris suum and caenorhabditis elegans indicate that in six of the mt-protein genes of a. suum and three of the mt-protein genes of c. elegans ttg is used as the translation initiation codon. also, gtt seems to be the translation initiation codon of the a. suum coiii gene. all of the five remaining a. suum mt-protein genes appear to begin with att and the remaining nine c. elegans mt- ...	1990	2235493
effects of multiple dose infections with ascaris suum on blood gastrointestinal hormone levels in pigs.	ten consecutive daily doses of infective ascaris suum eggs were administered to pigs in two experiments and the levels of gastrointestinal hormones in their blood were measured. the piglets in each experiment were divided into low-dose (ldi) and high-dose (hdi) infections and control groups. infected pigs had lower feed consumption, lower weight gains, and lower feed efficiency than control pigs. serum gastrin levels in infected pigs were significantly lower than the controls from days 7 to 17 p ...	1990	2238428
[trypsin and alpha-amylase activities in the pancreas of guinea pigs. ii. effect of per os and intra-intestinal infection with invasive ova of ascaris suum].	the guinea pigs were infected orally or intraintestinally (after laparotomy) with 5000 invasive eggs of ascaris suum. on the 7th day after infection the invasion was controlled by lung's weight, degree of congestion, and number of larvae in the lungs. the activity of amylase was determined according to fennel method, trypsin to anson method. the activities of both enzymes in pancreas of orally infected animals (after laparotomy or not) were lower, and relative weight of lungs of these animals wa ...	1990	2256335
[trypsin and alpha-amylase activities in the pancreas of guinea pigs. iii. effect of homogenates of invasive ova of ascaris suum and organic acids c2-c6 on the activity of enzymes in pancreatic extracts].	the studies were carried out on pancreas extracts from guinea pigs free of parasite invasion. the activity of trypsin was determined according to the method of anson, and amylase of fennel method. the measurements of activity were done at the presence of homogenized invasive eggs of ascaris suum, and the mixture of volatile organic acids c2-c6 (at concentrations of 30.3 and 0.3 mm/l). in samples with homogenized invasive eggs of a. suum the amylase activity remained unchanged while the activity ...	1990	2256339
[helminth infections in wild boars in enclosures in southern germany: species spectrum and infection frequency].	to determine the helminth fauna of wild boars, stomachs and intestines of 124, lungs of 62, and livers of 39 animals from 5 enclosures in southern germany were examined. the evaluation of the species composition revealed at least 13 species: metastrongylus apri, m. salmi, m. pudendotectus, physocephalus sexalatus, hyostrongylus rubidus, globocephalus longemucronatus, ascaris suum, trichuris suis, oesophagostomum dentatum, o. quadrispinulatum, fasciola hepatica, c. tenuicollis and capillaria. the ...	1990	2264058
molecular cloning and expression of an immunodominant 53-kda excretory-secretory antigen from trichinella spiralis muscle larvae.	a trichinella spiralis cdna expression library was constructed in lambda gt11 from muscle larvae mrna and immunologically screened to identify genes encoding previously described immunodiagnostic excretory-secretory (es) antigens. screening the library with t. spiralis infection serum from swine or rabbit antiserum to t. spiralis es antigen identified one clone, designated tsa-12, that contains a cdna transcript 539 bp in length and codes for an apparent 123-kda beta-galactosidase fusion protein ...	1990	2270099
effect of nonadrenergic noncholinergic inhibitory nerve stimulation on the allergic reaction in cat airways.	to examine the effect of nonadrenergic noncholinergic (nanc) inhibitory nerve stimulation on the antigen inhalation with allergic animals, changes in pulmonary resistance (rl) and arterial plasma histamine concentration ([h]) caused by inhalation of ascaris suum antigen were studied in five control (group a) and five nerve-stimulated (group b) cats, which were anesthetized and mechanically ventilated. all animals were actively sensitized with ascaris antigen before the experiment. after choliner ...	1990	2297183
acute topical steroid administration blocks mast cell increase and the late asthmatic response of the canine peripheral airways.	previous studies indicated that mast cell number increased after airway exposure to ascaris suum antigen (ag). the following two series of experiments were performed to test the hypothesis that this phenomenon may be associated with the ag-induced late-phase bronchoconstriction (lar). in the first, two bronchoscopes wedged in airway segments of two contralateral lobes of 16 dogs were used to deliver 0.26, 2.6, or 26 micrograms of ag to one lobe; the other served as a control. after the observati ...	1990	2301857
penetrating the conjunctival barrier. the role of molecular weight.	dinitrophenyl (dnp) derivatives of various molecular weights were tested for their ability to elicit ocular anaphylaxis after topical application to the eye of immunized animals. adult male sprague-dawley rats were immunized by intraperitoneal injection of dnp-ascaris suum extracts and alum and were then skin-tested with dnp-bovine serum albumin on day 13 post-immunization to assess their sensitivity to the dnp hapten. on day 14, animals were challenged topically with dnp derivatives in one eye; ...	1990	2303328
capsaicin and histamine antagonist-sensitive mechanisms in the immediate allergic reaction of pig airways.	the airway vascular and bronchial responses were studied in pigs sensitized with ascaris suum. ascaris, histamine (h) and capsaicin aerosol all induced a clear-cut increase in blood flow in the nasal, laryngeal and bronchial circulation with a decrease in vascular resistance of 20-40%. when delivered to the lung both ascaris and histamine, but not capsaicin, caused pulmonary airflow obstruction with increase in resistance and a fall in dynamic compliance of 40-70%. after pretreatment of pigs wit ...	1990	2309568
inhibitory effect of renal nerve activity during canine anaphylactic hypotension.	we have examined baroreceptor regulation of renal nerve activity (rna) during anaphylactic hypotension that affects renal nerve activity. in anesthetized dogs, mean blood pressure (mbp), heart rate (hr), and rna were simultaneously measured. to test for a normally functioning baroreceptor reflex in each animal, a transient hypotension was induced by an intravenous injection of sodium nitroprusside (4 micrograms/kg). nitroprusside produced a reflex increase in rna (+63 +/- 12% at -20 mmhg and +13 ...	1990	2309932
in vitro induction of crawling in the amoeboid sperm of the nematode parasite, ascaris suum.	in a highly synchronous process, the immotile spermatids of ascaris suum extend pseudopods and become rapidly crawling sperm when treated with an extract from the glandular vas deferens of the male under strict anaerobic conditions. within 9-12 min, a pseudopod develops, elongates rapidly, and exhibits a continuous flow of membrane specializations, the villipodia, from tip toward base. when attached to acid-washed glass, the pseudopod pulls the cell body along at speeds exceeding 70 microns/min. ...	1990	2311127
evaluation of pyrantel-tartrate abbreviated ascaris suum infections for the development of resistance in young pigs against migrating larvae.	crossbred young pigs were used to test whether abbreviated infections with eggs of ascaris suum can stimulate the acquisition of resistance to challenge. weanling pigs from an ascaris-free colony were kept free of a. suum until they were divided into groups at the age of 7-8 weeks. the experimental animals received pyrantel tartrate during the period when they were being exposed to increasing numbers of infective a. suum eggs and challenged 10 days after the last infective dose. liver milk-spot ...	1990	2312230
putrescine n-acetyltransferase in onchocerca volvulus and ascaris suum, an enzyme which is involved in polyamine degradation and release of n-acetylputrescine.	a novel type of n-acetyltransferase, clearly different from the nuclear and cytosolic polyamine n-acetyltransferases of mammals, was recently found in the intestinal nematode ascaris suum. the occurrence of this putrescine n-acetylating enzyme has also been noted in the filarial parasite onchocerca volvulus. the enzyme was partially purified from adults of o. volvulus and a. suum by chromatography on deae-cellulose and cadaverine-sepharose. substrate specificities of the filarial enzyme resemble ...	1990	2320051
structural studies on three flavin-interacting regions of the flavoprotein subunit of complex ii in ascaris suum mitochondria.	the flavoprotein (fp) subunit of mitochondrial complex ii contains covalently bound fad as a prosthetic group. in this study, the primary structure of the flavin-bound tryptic peptide from the fp subunit of ascaris complex ii was determined and found to be highly similar to those of the corresponding flavin-binding regions of bovine heart and bacterial fp subunits. furthermore, the ascaris fp subunit was shown to contain two regions exhibiting striking sequence similarity to the segments that ha ...	1990	2335235
n-terminal amino acid sequence identity between a major allergen of ascaris lumbricoides and ascaris suum, and mhc-restricted ige responses to it.	a protein allergen of the parasitic nematode ascaris has been purified to homogeneity by immunoaffinity chromatography. it is the most abundant protein species in the parasite's body fluid and has been named aba-1. the allergen's molecular weight (mw) has been previously estimated at 14,000, but this sizing is currently under re-evaluation. the immunological activity of the protein was intact after purification, as attested by immunoprecipitation and passive cutaneous anaphylaxis. the ige respon ...	1990	2335378
experimental pulmonary eosinophilia in mice by ascaris suum extract.	the transnasal administration of an extract of the parasite ascaris suum (asc) to c57bl/6 mice for 3 wk produced marked eosinophilia in the bronchoalveolar lavage (bal) fluid. this pulmonary eosinophilia was not accompanied by blood eosinophilia. the oral administration of cyclosporin, 50 mg/kg body weight, every other day significantly suppressed the pulmonary eosinophilia. athymic c57bl/6-nu/nu mice failed to develop pulmonary eosinophilia. these data indicate that pulmonary eosinophilia cause ...	1990	2339848
acquired resistance to migrating larvae of ascaris suum in young pigs by repeated drug-abbreviated infections.	cross-bred 3- and 8-wk-old pigs were used to test whether drug-abbreviated infections with ascaris suum can stimulate acquired resistance to challenge. during the immunization period, both age groups of animals were infected with increasing numbers of a. suum eggs (500, 1,000, 2,000, 5,000, 10,000, and 20,000) at 7-day intervals while the pigs were receiving pyrantel tartrate in the feed. two days after the last infective dose, animals were placed on unmedicated feed for 8 days and then challeng ...	1990	2352069
the specificity of the antibody response to internal antigens of ascaris: heterogeneity in infected humans, and mhc (h-2) control of the repertoire in mice.	children from an area of africa endemic for the large roundworm of humans, ascaris lumbricoides, were found to vary considerably in the specificity of their serum igg response to the internal antigens of the parasite. this was particularly noticeable for responses to a 14-kd protein (aba-1) of the parasite that has previously been shown to be the subject of a strong ige antibody response in infected animals. the possibility that this heterogeneity in immune repertoire has a genetic basis was exp ...	1990	2357848
elisa detection of o. volvulus antigenemia in snip-positive patients by monoclonal antibodies directed against a 56 kd glycoprotein of ascaris suum.		1990	2378214
tumor necrosis factor-like cytotoxicity and anorexia in ascaris suum infected pigs.	tumor necrosis factor-like cytotoxicity (tnf-lc) was examined in sera from 12 pigs experimentally infected with ascaris suum. the difference of tnf-lc levels between eight infected and four uninfected controls was not significant. when an endotoxin challenge was intravenously administered 1 month after the first dose of a. suum, the levels of tnf-lc in the sera of infected pigs were one-third that of the controls 125 min post-challenge (pc). in a more detailed study on four infected and two unin ...	1990	2382388
ozone increases susceptibility to antigen inhalation in allergic dogs.	to determine whether o3 exposure increased airway responsiveness to antigen inhalation, we studied airway responsiveness to acetylcholine (ach) and ascaris suum antigen (aa) before and after o3 in dogs both sensitive and insensitive to aa. airway responsiveness was assessed by determining the provocative concentration of ach and aa aerosols that increased respiratory resistance (rrs) to twice the base-line value. o3 (3 parts per million) increased airway responsiveness to ach in dogs both sensit ...	1990	2384406
biotin as a probe of the surface of ascaris suum developmental stages.	sulfo-nhs-biotin (aqueous soluble) and nhs-biotin (organic soluble) labeled similar sds-2me (sodium dodecyl sulfate/beta-mercaptoethanol) soluble cuticular proteins of second stage larvae (l2) and third stage ascaris suum larvae (l3). comparable analysis of biotin-labeled fourth stage larvae (l4), young adults, and mature adult ascaris suum revealed strong labeling of several sds-2me soluble cuticular proteins with nhs-biotin, while sulfo-nhs-biotin appeared to strongly label a single sds-2me so ...	1990	2385267
[analysis of antigens defined by anti-anisakis larvae antibodies of ige and igg type in the sera of patients with acute gastrointestinal anisakiasis].	using sds-page and immunoblotting assay, we analysed antigens defined by anti-anisakis larvae antibodies of ige and igg type in the sera of patients with acute gastrointestinal anisakiasis. 1) humoral ige response to 76, 64 and 60 kda molecular weight proteins of anisakis larvae and igg response to 91, 76, 64 and 60 kda molecular weight proteins were demonstrated in the sera of patients with acute gastric anisakiasis. those humoral antibody responses were also demonstrated in the sera of two pat ...	1990	2388423
biophysical transport properties of the cuticle of ascaris suum.	the transport properties of isolated cuticle from ascaris suum were studied using standard two-chamber diffusion cells and a number of radiolabeled permeants which varied in molecular size, lipophilicity and electrical charge. the permeability coefficient of the collagen matrix (lipid-extracted cuticle) vs. molecular radius relationship showed the interdependence of molecular size and electrical charge of the permeants with respect to the aqueous pores of the negatively charged matrix. the perme ...	1990	2398915
kinetic mechanism of nad:malic enzyme from ascaris suum in the direction of reductive carboxylation.	initial velocity studies in the absence and presence of product and dead-end inhibitors suggest a steady-state random mechanism for malic enzyme in the direction of reductive carboxylation of pyruvate. for this quadreactant enzymatic reaction (mn2+ is a pseudoreactant), initial velocity patterns were obtained under conditions in which two substrates were maintained at saturating concentrations while one reactant was varied at several fixed concentrations of the other. data from the resulting rec ...	1991	1993653
antigen-induced phospholipase d activation in rat mast cells is independent of protein kinase c.	in the present study, we investigated the involvement of protein kinase c (pkc) in antigen (ag, dnp-ascaris suum)-induced phospholipase d (pld) activation of rat peritoneal mast cells. phorbor myristate acetate (pma) as well as ag activated pld as inferred by phosphatidylethanol (pet) production. pkc inhibitors, staurosporine and h-7, however, failed to suppress pma-stimulated pld activation, suggesting that pld activation by pma is independent of pkc. by contrast, ag-stimulated pld activity was ...	1991	1998501
effects of single and multiple inhalations of antigen on airway responsiveness in monkeys.	airway hyperresponsiveness is an important and characteristic feature of asthma. in monkeys, we have reported that antigen (ag) inhalation induces a prolonged airway eosinophilia, that chronic airway eosinophilia is associated with marked airway hyperresponsiveness, and that chronic ag inhalations induce airway eosinophilia and hyperresponsiveness. in this study we have determined the effects of acute ag inhalation(s) on airway responsiveness to obtain a protocol for the study of the mechanisms ...	1991	2013678
separation of late bronchial responses from airway hyperresponsiveness in allergic sheep.	allergic sheep with antigen-induced early and late responses were used to determine whether airway hyperresponsiveness (ahr) to carbachol is present during the late response and whether blocking the late response with the leukotriene d4 (ltd4) antagonist mk-571 also blocks this ahr. to do this, we first showed that mk-571 blocked the antigen-induced late response, and then, in a separate study, we determined the effect of mk-571 treatment on airway responsiveness 6 h after antigen challenge (at ...	1991	2022553
modification of a thiol at the active site of the ascaris suum nad-malic enzyme results in changes in the rate-determining steps for oxidative decarboxylation of l-malate.	a thiol group at the malate-binding site of the nad-malic enzyme from ascaris suum has been modified to thiocyanate. the modified enzyme generally exhibits slight increases in knad and ki metal and decreases in vmax as the metal size increases from mg2+ to mn2+ to cd2+, indicative of crowding in the site. the kmalate value increases 10- to 30-fold, suggesting that malate does not bind optimally to the modified enzyme. deuterium isotope effects on v and v/kmalate increase with all three metal ion ...	1991	2043616
characterization of cdna clones for the beta subunit of pyruvate dehydrogenase from ascaris suum.	the pyruvate dehydrogenase complex occupies a unique position in the anaerobic mitochondrial metabolism of the parasitic nematode ascaris suum. this paper describes cdna clones for the beta subunit of the pyruvate dehydrogenase component of this complex. a cdna library has been constructed in lambda gt11 from poly(a)+ rna isolated from adult ascarid body wall muscle. the library was screened with antiserum prepared against the beta subunit of pyruvate dehydrogenase. full-length clones of 1.2 kb ...	1991	2052042
neuropeptides in the nematode ascaris suum.		1991	2057216
airway effects of inhaled bradykinin, substance p, and neurokinin a in sheep.	the effects of inhaled bradykinin (bk), substance p (sp), and neurokinin a (nka) on pulmonary resistance and airway responsiveness to carbachol were studied in conscious allergic sheep. inhaled bk (20 breaths, 0.1 to 5.0 mg.ml-1) caused dose-dependent increases in pulmonary resistance. neither inhaled sp nor nka (20 breaths, 0.1 to 1.0 mg.ml-1) produced significant bronchoconstriction in allergic sheep. however, the response to sp could be enhanced (p less than 0.05) by pretreatment with the neu ...	1991	1704388
the complete primary structure of a nematode alpha 2(iv) collagen and the partial structural organization of its gene.	we have isolated and characterized cdna and genomic dna clones which encode an alpha 2(iv) collagen chain from the parasitic nematode ascaris suum. in addition we have determined, by nucleic acid sequence analysis, the structural organization of approximately two-thirds of the gene. this analysis has shown that the gene contains at least 15 introns, and those that have been characterized range in size from 141 to 854 base pairs. the derived protein sequence contains 1763 amino acids and includes ...	1991	1714907
leukotrienes, thromboxane a2, and prostaglandins during systemic anaphylaxis in sheep.	we investigated the roles of eicosanoid mediators in acute systemic anaphylaxis in anesthetized sheep. sheep were sensitized with dinitrophenylated ascaris suum extract and were challenged with an intravenous injection of dinitrophenylated bovine serum albumin. during anaphylaxis, cyclooxygenase inhibitors eliminated the elevation of arterial plasma levels of thromboxane b2 and 6-ketoprostaglandin f 1 alpha but markedly elevated the levels of leukotriene e4 in lung lymph without significantly el ...	1991	1716064
studies on the ionic selectivity of the gaba-operated chloride channel on the somatic muscle bag cells of the parasitic nematode ascaris suum.	the reversal potential for the gaba-elicited hyperpolarization (egaba) of the muscle bag cells of the parasite nematode ascaris suum is near to the equilibrium potential for chloride ions (ecl) indicating that this is a chloride-mediated event. the value of egaba in the presence of different anions indicates the selectivity sequence of the gaba-operated chloride channel as scn- greater than br-, i- greater than clo3-, no3-, cl- greater than c2h5coo- greater than ch3coo-, hcoo-, ohch2ch2so3h-, ch ...	1991	1716924
larval exoantigens from ascarid nematodes are potent inducers of histamine release from human blood basophils.	histamine release (hr) from washed human blood cells after challenge with the excretory-secretory antigens (es) of the parasitic nematodes toxocara canis and ascaris suum was studied, employing a recently developed microfibre-based assay combined with hyperosmolar release media for maximal sensitivity. blood samples were obtained from 30 patients suspected of parasite infection and 11 healthy volunteers serving as controls. specific antibodies of ige, igg1 and igg4 subclasses were determined by ...	1991	1723345
structure of mitochondrial trna.	some mitochondrial (mt) trnas are presumed to have unusual secondary structures on the basis of dna sequence analyses. however there are few trnas whose structures have been investigated at rna level. thus we isolated some mt trnas to investigated their structures and also synthesized mt trna by run-off transcription. in these experiments, following results have been obtained. 1) bovine mt trna(ser)(agy) needs the interaction between the t-loop and d-arm for its aminoacylation activity. 2) the s ...	1991	1726807
in vitro damaging effect of leukocytes, serum, and peritoneal fluid on ascaris suum infective larvae.	peritoneal leukocytes, serum, and peritoneal fluid from ascaris suum-infected or noninfected mice were studied using 51cr release and larval mobility to determine if they had in vitro cytotoxicity for a. suum infective larvae. mouse serum or peritoneal fluid from infected and non-infected mice was added to leukocyte-larvae cultures to determine their effect on leukocyte damage to larvae. leukocytes from a. suum infected mice were cytotoxic for a. suum larvae in the presence of serum from infecte ...	1991	1741924
morphological and morphometric studies of the airways of sheep with acute airway hypersensitivity to inhaled ascaris suum.	the airways of 12 sheep with naturally-occurring allergic airway hypersensitivity, six of which had changes in both airway resistance and dynamic lung compliance (group a) and six of which had changes in only dynamic lung compliance (group b), were compared quantitatively with six non-reacting sheep (group c) in order to examine the relation between airway hypersensitivity and various morphological features thought to be related to airway hypersensitivity. compared to the non-reacting sheep (gro ...	1991	1742207
lysophospholipase activity of ascaris suum-induced mouse peritoneal neutrophils and eosinophils.	lysophospholipase activity of mouse peritoneal neutrophils and eosinophils was studied to determine if neutrophils and eosinophils have lysophospholipase activity when treated with ascaris suum whole worm extract, if zymosan activated complement can induce increased lysophospholipase activity, or if the immune status of the host has an effect on lysophospholipase activity. neutrophils from noninfected or infected (immunized) mice were found to have increased lysophospholipase activity when treat ...	1991	1753875
phospholipids and protein kinase c in acetylcholine-dependent signal transduction in ascaris suum.	quantitatively, the major phospholipid in the muscle of the nematode ascaris suum was found to be phosphatidylcholine (lecithin). stimulation of ascaris muscle with acetylcholine or the agonists carbachol and levamisole increased the level of phosphorylcholine, 1,2-diacylglycerides and phosphatidic acid. increased levels of these compounds, together with the demonstration of phospholipase c activity, suggest that phospholipid hydrolysis may be associated with the ach response of the muscle via s ...	1991	1762627
structure of the polypeptide chain of extracellular hemoglobin from the nematode ascaris suum.	1. ascaris suum extracellular hemoglobin is composed of eight identical single polypeptide chain subunits carrying two heme binding sites each. 2. limited trypsinolysis followed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis gave a major band corresponding to half the molecular mass of an intact subunit. 3. peptide mapping of tryptic hydrolysates yielded 27 to 30 fluorescamine positive spots, about half the number of lysyl and arginyl residues in a polypeptide chain. 4. the findin ...	1991	1764923
centripetal flow and directed reassembly of the major sperm protein (msp) cytoskeleton in the amoeboid sperm of the nematode, ascaris suum.	the cytoskeleton of the amoeboid spermatozoa of ascaris suum consists of major sperm protein (msp) filaments arranged into long, branched fiber complexes that span the length of the pseudopod and treadmill rearward continuously due to assembly and disassembly at opposite ends of the complexes (sepsenwol et al., journal of cell biology 108:55-66, (1989)). examination by video-enhanced microscopy showed that this cytoskeletal flow is tightly coupled to sperm locomotion. the fiber complexes treadmi ...	1991	1773449
[effects of immunoactivity on ascaris suum infection in mice].	the immune response to sheep red blood cell (srbc) was monitored in the mice infected with ascaris suum or trichinella spiralis. the effects of the infection with t. spiralis or the injection with cyclophosphamide(cy) as an immunosuppression agent prior to challenge infection with the embryonated eggs of a. suum were monitored in mice by means of the level of infection with a. suum and cellular and humoral immune response to srbc. following the oral administration of 1,000 eggs of a. suum to mic ...	1991	1786254
identification of tubulin isoforms in different tissues of ascaris suum using anti-tubulin monoclonal antibodies.	three monoclonal antibodies specific to alpha- and beta-tubulin were used to examine the expression of tubulin isoforms in the intestine, reproductive tract and body wall muscle of a. suum. the tubulins were found to be different in their isoelectric points, number of isoforms and peptide maps with western blot analysis of one-dimensional polyacrylamide gel confirming the presence of alpha-, beta 1- and beta 2-tubulin. commercial cross-reactive anti-alpha and anti-beta mabs 356 and 357 recognize ...	1991	1787032
[helminth infections in wild boars kept in enclosures in southern germany: severity of infections and fecal intensity].	to determine the intensity of helminth infection and egg output with regard to age and sex of wild boar, stomachs and intestines of 124, lungs of 62, and livers of 39 animals from 5 enclosures in southern germany were examined. the evaluation of the intensity of infection showed 579.4 worms per animal for hyostrongylus rubidus, 510.4 for globocephalus longemucronatus, 476.9 for oesophagostomum dentatum, 476.9 for o. quadrispinulatum, 254.2 for metastrongylus pudendotectus, 176.9 for m. salmi, 14 ...	1991	1796467
airway inflammation in sheep with acute airway hypersensitivity to inhaled ascaris suum.	in order to investigate the relationship between airway inflammation and the development of naturally occurring allergic airway hypersensitivity, the lower respiratory tract was studied in eighteen sheep 14 days after challenge with inhaled ascaris suum antigen. sheep could be classed into three groups on the basis of their response: group a (n = 6) had significant changes in both airway resistance and dynamic lung compliance; group b (n = 6) had changes in only dynamic lung compliance, and grou ...	1991	1804794
[trypsin and alpha-amylase activity in the pancreas of guinea pigs. iv. comparison of the effects of administration of the mixtures of volatile organic acids, invasive ova of ascaris suum and their homogenates].	the activity of alpha-amylase in the experimental groups of guinea pigs was lower than in control animals. the decreased level of activity of trypsin was noted (p less than 0.05) also in infected animals, and in treatment with organic acids. in the group of guinea pigs, which was administrated homogenized eggs of parasite, the activity of enzyme was a little higher than in control animals. the infection of animals was associated with increase of relative weight of lungs. the guinea pigs treatmen ...	1991	1822040
[effects of administration of vitamin a, b 2 and b 15 to guinea pigs during the larval stage of ascariasis on alpha-amylase activity in the lungs and kidneys].	the guinea pigs were administrated vit. a (400 i.u.), vit. b2 (1 mg) or vit. b15 (5 mg). on the 9-th day of the experiment part of them was infected with 5000 invasive eggs of ascaris suum. the invasion lasted 6 days and was controlled by lungs and kidney weight, and number of larvae in the lungs. the activity of amylase was determined by saccharogenic method in both organs. in the lungs of infected animals the activity of alpha-amylase was about 3 times lower than in the control. the infection ...	1991	1822041
[trypsin and alpha-amylase activity in the pancreas of guinea pigs. v. effects of administration of vitamin a and b 2 during the larval stage of ascariasis].	the infection of guinea pigs with ascaris suum larvae resulted in decrease of the activities of trypsin and alpha-amylase, and in increase of lipase activity in extracts from their pancreas. the activity of alpha-amylase, lipase and the relative weight of lungs of infected animals which were given vitamin a, did not differ from control animals. the activity of trypsin from pancreas these animals was higher than that measured in only infected guinea pigs but it was lower than in control animals. ...	1991	1822049
[experimental evaluation of the degree of detectability of helminths' eggs in the soil using 2 methods].	sand and soil were experimentally contaminated with eggs of ascaris suum and oncospheres of hymenolepis diminuta in three doses: 500, 3000 and 5000 eggs (oncospheres) per 50 g of the ground. numbers of recovered eggs were compared with the use of the two following methods: the method of flotation and spindler's method, modified by vasilkova and ivancuk. the ground samples were investigated the following day and on the 14th day after the contamination. the samples were stored in 20 degrees c and ...	1991	1822050
current status of food-borne parasitic zoonoses in singapore.	parasitic infections adopt a rather low profile in the highly urbanized setting in singapore. very few food-borne parasitic infections are encountered. apart from a few reports of infections with clonorchis/opisthorchis, taenia spp. and hydatid disease, there are no other citations of such helminthic infections. seroprevalence surveys have shown the presence of toxoplasmosis in local meat animals (sheep, pigs and cattle) and toxoplasma strains have been isolated from the pig, tree shrew (tupaia ...	1991	1822903
suppression of the ige antibody response by ascaris suum components: effect of x-irradiation.	the effect of x-irradiation on the suppression of ige antibody responses induced by some of the ascaris suum (asc) components was analyzed in mice (7-week old a/sn females). treatment with 300 r 24 h before immunization with 50 micrograms ova and 200 micrograms asc suppressive components abolished the damping effect on anti-ova ige antibody levels. the same effect was observed on the anti-asc ige antibody response obtained in mice injected with 200 micrograms asc immunogenic plus 200 micrograms ...	1991	1823275
effector-induced conformational transitions in ascaris suum phosphofructokinase. a fluorescence and circular dichroism study.	results of activity and spectral studies using fluorescence and circular dichroism show that amp and fructose 2,6-bisphosphate (f-2,6-p2) activate ascaris suum phosphofructokinase through specific and similar conformational changes. inorganic compounds like (nh4)2so4 and kh2po4 also induce structural alterations in the enzyme in a manner different from those caused by amp and f-2,6-p2. the enzyme is activated by both amp and f-2,6-p2, in 20 mm phosphate buffer, ph 6.6, with 0.2 mm atp and 1 mm f ...	1991	1827437
fructose 2,6-bisphosphate and amp increase the affinity of the ascaris suum phosphofructokinase for fructose 6-phosphate in a process separate from the relief of atp inhibition.	kinetic data have been collected suggesting that heterotropic activation by fructose 2,6-bisphosphate and amp is a result not only of the relief of allosteric inhibition by atp but is also the result of an increase in the affinity of phosphofructokinase for fructose 6-phosphate. modification of the ascaris suum phosphofructokinase at the atp inhibitory site produces a form of the enzyme that no longer has hysteretic time courses or homotropic positive (fructose 6-phosphate) cooperativity or subs ...	1991	1827438
trypsin modification of phosphofructokinase from ascaris suum.	phosphofructokinase from ascaris suum is a tetramer with subunits of 90 kda. treatment of the native enzyme with trypsin (10%, w/w) followed by sds-gel electrophoresis was shown to immediately generate a 40-kda fragment followed by a gradual formation of two other fragments of 37 and 32 kda. the loss of catalytic activity during the digestion was less than 50%. gel filtration of the digested enzyme under non-denaturing conditions showed a mr almost that of the native enzyme. digestion of the pho ...	1991	1828862
identification and isolation of a specific antigen with diagnostic potential from dictyocaulus viviparus.	the purpose of the investigation was to isolate and identify a specific antigen of dictyocaulus viviparus that can be used to diagnose lungworm infections in cattle. somatic, excretion and secretion antigens of adult d. viviparus and somatic antigens of l3 larvae were examined in an indirect enzyme-linked immunosorbent assay (elisa) to determine whether they cross-reacted with sera collected from calves with mono-infections of fasciola hepatica. ostertagia ostertagi, ascaris suum, or cooperia on ...	1991	1832795
transmission of intestinal helminths in danish sow herds.	faecal samples from sows and their litters, all untreated with anthelmintics, were examined in eight sow herds, ranging from very intensive to very traditional management. four helminth genera were recorded, namely oesophagostomum sp., ascaris suum, trichuris suis and strongyloides ransomi. the herds with the most intensive management were only infected with a. suum, while the more traditionally managed herds were infected with three or four species. in one herd, the sows showed a small relative ...	1991	1832796
modification of the atp inhibitory site of the ascaris suum phosphofructokinase results in the stabilization of an inactive t state.	treatment of the ascaris suum phosphofructokinase (pfk) with 2',3'-dialdehyde atp (oatp) results in an enzyme form that is inactive. the conformational integrity of the active site, however, is preserved, suggesting that oatp modification locks the pfk into an inactive t state that cannot be activated. a rapid, irreversible first-order inactivation of the pfk is observed in the presence of oatp. the rate of inactivation is saturable and gives a koatp of 1.07 +/- 0.27 mm. complete protection agai ...	1991	1832959
n-acetylglucosaminyltransferase from ascaris suum.	the occurrence of n-acetylglucosaminyltransferase, the initial step in the synthesis of the carbohydrate moiety of n-linked glycoproteins, is demonstrated in the microsomal fraction of the nematode ascaris suum. phosphatidylglycerol stimulated enzyme activity three- to six-fold without affecting the km values of either substrates, uridinediphospho-n-acetylglucosamine or dolichylphosphate. the km values were determined to be about 12 microm and 100 micrograms ml-1, respectively. the enzyme activi ...	1991	1836781
[dissemination and survival of endoparasite embryos in the environment].	pig farms with the annual capacity from five to 20 thousand head represent one of the crucial fields of agriculture influencing the environment in the csfr. daily production of large amounts of liquid excrements (150-300 m3), as a result of high concentrations of animals, poses not only technical but also sanitary problems closely related to the animal and man's health. in a long-term (267 days) field study the authors followed the development and survival of eggs of the model helminth ascaris s ...	1991	1841477
initial characterization of several actin genes in the parasitic nematode, ascaris suum.	southern hybridization data suggest a large actin gene family in ascaris suum. our genomic reconstruction experiment indicated that it consists of 50-75 members. polymorphism was uncovered in the actin genes or in their surrounding sequences. from the genomic library 5 nonoverlapping actin clones were isolated and characterized.	1991	1859418
cut-1 a caenorhabditis elegans gene coding for a dauer-specific noncollagenous component of the cuticle.	we have molecularly identified a new gene of caenorhabditis elegans that codes for a component of the cuticle. the gene has been physically mapped on lgii near the locus sqt-1. the structure and the sequence of the gene have been determined and antisera have been raised against parts of the protein produced as fusions in escherichia coli. by transcription analysis, and by the use of specific antisera, we have determined that this gene is expressed specifically during dauer larva formation. in ex ...	1991	1864469
distribution of 3h-gaba uptake sites in the nematode ascaris.	the distribution of uptake sites for the inhibitory neurotransmitter gamma-aminobutyric acid (gaba) in the nematode ascaris suum was examined by autoradiography of 3h-gaba uptake. single neural processes in both the ventral and dorsal nerve cords were labeled with 3h-gaba. serial section analysis identified the cells of origin of these processes as the rmev-like and rmed-like neurons. these cells belong to a set of four neurons in the nerve ring, all of which are labeled by 3h-gaba. 3h-gaba labe ...	1991	1869634
stimulation of leukocyte lysophospholipase activity by noninfectious agents.	mouse peritoneal leukocyte lysophospholipase (lpl) activity was studied to determine whether or not noninfectious agents cause increased enzyme activity and whether neutrophils have lpl activity. in the first study, mice infected with ascaris suum, a known inducer of lpl activity, were given intraperitoneal injections of proteose peptone, thioglycolate, bovine albumin, paraffin, glycogen, or a. suum whole worm extract (wwe). cell populations collected from mice injected with a. suum wwe, proteos ...	1991	1871154
nonspecific immunomodulation influences resistance of mice to experimental infection with mesocestoides corti and ascaris suum.	the influence of nonspecific immunomodulation on the course of experimental infection was examined in larval cestodosis (mesocestoides corti) and ascaridosis (ascaris suum) in mice. immunosuppressive treatment (with azathioprine or hydrocortisone) resulted in a decrease of resistance in both models. the subsequent administration of t-activin to immunosuppressed mice led to the restoration of resistance to a level equal to that of untreated control mice. the administration of different immunomodu ...	1991	1880386
trichinella spiralis: a 76-kda excretory/secretory larval antigen identified by a monoclonal antibody.	spleen cells from balb/c mice were fused with myeloma cells following infection of the mice with trichinella spiralis larvae and an ip booster injection with larval homogenate antigen. a monoclonal antibody (mab), designated as ts 3g6 which did not react with sera or tissue extracts from noninfected mice, rats, and guinea pigs, was selected for further studies because of its high activity and specificity. when tested in elisa ts 3g6 did not cross-react with ascaris suum, a. lumbricoides, toxocar ...	1991	1889470
trichinella spiralis: light microscope monoclonal antibody localization and immunochemical characterization of phosphorylcholine and other antigens in the muscle larva.	a panel of monoclonal antibodies was used to examine the structure of the muscle larva of trichinella spiralis under the light microscope. immunofluorescence and, in some cases, immunoperoxidase staining were used. all four antibodies reacted with the cuticle of the organism, although differences in the staining pattern were observed for some of these. interestingly, all the antibodies also reacted with the stichosome. one of the antibodies (ts2ab) is specific for the hapten, phosphorylcholine. ...	1991	1889472
a seroepidemiologic survey of human dirofilariosis in western spain.	the objective of this work is to define the prevalence of igm and igg antibodies against dirofilaria immitis in an exposed human population. 395 individuals attending primary care facilities in an area endemic with canine dirofilariasis were studied, as well as 100 non exposed individuals. an elisa method was used for the detection of igg and igm antidirofilarial antibodies. sera were preadsorbed with antigens of toxocara canis, ascaris suum, echinoccocus granulosus and fasciola hepatica to prev ...	1991	1896765
airway blood flow modifies allergic airway smooth muscle contraction.	we tested the hypothesis that airway perfusion modifies the contractile response of airway smooth muscle to allergen challenge by influencing the clearance of locally released spasmogens. in six intact, lightly sedated, sheep allergic to ascaris suum, we measured tracheal mucosal blood flow (qtr) with a soluble gas uptake method and tracheal dead space (vtr), an index of airway smooth muscle tone, by helium dilution before and serially after local aerosol challenge with a. suum extract or ragwee ...	1991	1905892
[livestock manure as a vector for parasites--a report of experiences].	analysing the results of own investigations and informations in literature a review is given about the role of manure specially in stables of high-intensity cattle and pig production as a vector for exogenic parasite stages. in the course of investigations--in most cases simulating practice conditions--so-called indicator germs were used as test material. in cattle stables manure is significant as a vector for eimeria species, cryptosporidium parvum, sarcocystis species, taenia saginata and fasc ...	1991	1914947
molecular symmetry and arrangement of subunits in extracellular hemoglobin from the nematode ascaris suum.	the arrangement of subunits and molecular symmetry of extracellular hemoglobin from the nematode ascaris suum, an 11.7s molecular of molecular mass 332 kda and composed of eight identical subunits, was studied. dissociation of the molecule at alkaline and acid ph yielded 4.6s and 2.7s components, identified as polypeptide-chain dimers and monomers, respectively. cross-linking with glutardialdehyde followed by sds/page resulted in a maximum number of eight bands identified in order of decreasing ...	1991	1915360
ascaris suum: stage-specific differences in lectin binding to the larval cuticle.	a glycosylated component with affinity for wheat germ agglutinin (specific binding to n-acetyl-d-glucosamine monomers and oligomers) and weak affinity for poke weed mitogen (specific binding to n-acetyl-d-glucosamine oligomers) was detected temporally on the surface of ascaris suum larvae developing in vitro and on in vivo-derived larvae. the component was identified on the surface of in vitro-derived late second stage larvae, on all late third stage larvae (derived from pig lung), and all fourt ...	1991	1915752
the pharmacology of cholinoceptors on the somatic muscle cells of the parasitic nematode ascaris suum.	1. acetylcholine (ach) elicited depolarization and an increase in input conductance of the somatic muscle cells of the parasitic nematode ascaris suum. 2. the relative potency of nicotinic and muscarinic agents was studied in this preparation. the order of potency of these compounds was metahydroxy-phenylpropyltrimethylammonium (hppt) greater than 1.1 dimethyl-4-phenylpiperazinium (dmpp) greater than ach greater than carbachol greater than nicotine greater than tetramethylammonium (tma+) greater ...	1991	1919416
t-cell-dependent accumulation of eosinophils in the lung and its inhibition by monoclonal anti-interleukin-5.	the transnasal administration of an extract of the parasite ascaris suum to c57bl/6 mice for 3 weeks produced marked eosinophilia in the bronchoalveolar lavage (bal) fluid. the oral administration of ciclosporin significantly suppressed the pulmonary eosinophilia. athymic c57bl/6-nu/nu mice failed to develop pulmonary eosinophilia. these data indicate that the pulmonary eosinophilia caused by this parasite extract is t-cell-dependent. genetically mast-cell-deficient (wb x c57bl/6) f1-w/wv (w/wv) ...	1991	1937869
metabolism and fate of ecdysteroids in the nematodes ascaris suum and parascaris equorum.	when injected with [3h]ecdysone and maintained in vitro, the parasitic nematodes, ascaris suum and parascaris equorum each produced a series of polar and relatively apolar metabolites. a. suum metabolised the compound into ecdysonoic acid ([3h]eoic), ecdysone 25-glucoside ([3h]e25gluc), putative ecdysone 22-phosphate ([3h]e22p) and a series of at least six relatively apolar metabolites. all of these, except ecdysonoic acid, were hydrolysed by a crude enzyme preparation from helix pomatia, releas ...	1991	1944416
performance of pigs with mixed nematode infections before and after ivermectin treatment.	ninety crossbred parasitized pigs were used in two controlled experiments to compare performance before and after ivermectin treatment. four groups of 15 pigs were sequentially infected with 2000 ascaris suum, 10,000 oesophagostomum spp. and 10,000 strongyloides ransomi beginning at average pig weights of either 38 kg (experiment i) or 14 kg (experiment ii). two infected groups of pigs were treated with ivermectin on day 34 (experiment i) or 37 (experiment ii) of the experiments and all pigs wer ...	1991	1957485
the physiology and pharmacology of neuromuscular transmission in the nematode parasite, ascaris suum.	the organization of ascaris motoneurones and nervous system is summarized. there is an anterior nerve ring and associated ganglia, main dorsal and ventral nerve cords which run longitudinally, and a small set of posterior ganglia. cell bodies of motoneurones are found in the ventral nerve cord and occur in 5 repeating 'segments'; each contains 11 motoneurones. seven morphological types of excitatory or inhibitory motoneurone are recognized. each ascaris somatic muscle cell is composed of the con ...	1991	1647516
antigen-induced mediator release in primates.	we examined the release of bronchoactive mediators into the airways of allergic primates during the acute response to specific antigen inhalation. twelve adult male cynomolgus monkeys (macaca fascicularis) with a naturally occurring respiratory sensitivity to inhaled ascaris suum extract were anesthetized and intubated for each study. respiratory system resistance (rrs) and dynamic lung compliance (cldyn) were measured before and after antigen inhalation, and the release of mediators into the ai ...	1991	1648317
characterization of sulfidopeptide leukotriene responses in sheep tracheal smooth muscle in vitro.	we have investigated the effects of leukotrienes (lts) on isolated tracheal smooth muscle from sheep sensitive to ascaris suum antigen. ltc4 and ltd4 produced dose-dependent contractions of sheep trachea, but lte4 was virtually inactive. ym-17690, a non-analogous lt agonist, produced no contractile response up to 100 microm. indomethacin (5 microm) had no effect on ltc4- and ltd4-induced contractions. l-serine borate (45 mm), an inhibitor of gamma-glutamyl transpeptidase, shifted the dose-respon ...	1991	1655181
antagonist properties of arylaminopyridazine gaba derivatives at the ascaris muscle gaba receptor.	1. in a previous study, it was shown that the potency order for two arylamino-pyridazine derivatives, sr95531 and sr95103, was different in ascaris suum when compared to vertebrate preparations. sr95531, the most potent analogue at the vertebrate gabaa receptor, was found to be very weak at antagonizing gaba responses in ascaris, but sr95103, approximately 20 times less potent than sr95531 in vertebrate preparations, was more potent than sr95531 in ascaris. these results suggested the existence ...	1991	1658181
novel regulation of pyruvate dehydrogenase phosphatase activity from mitochondria of the parasitic nematode, ascaris suum.		1991	1664050
hallucinogenic and neuroleptic drug interactions with potential neurotransmitter receptors in parasitic nematodes.	receptors potentially involved in neurotransmitting have been characterised in the muscle tissue and in whole worms of the nematodes ascaris suum and onchocerca volvulus, respectively. binding studies revealed a high affinity for lsd with apparent kd values of 94 nm for a. suum and 120 nm for o. volvulus, whereas those of the neuroleptics haloperidol, spiperone and mianserin were found to be in the micromolar range. a variety of neurotransmitter antagonists, known to bind with high affinities ei ...	1991	1674821
effects of plumbagin on development of the parasitic nematodes haemonchus contortus and ascaris suum.	1. plumbagin (5-hydroxy,2-methyl-1,4-napthoquinone) inhibited the motility and survival of haemonchus contortus first-stage larvae (l1) with an ed50 of 1 microgram/ml, but was less effective in preventing the development of h. contortus to infective third-stage larvae in a faecal slurry assay. 2. of the structural analogs tested, plumbagin was the most potent in preventing development of l1 followed in decreasing order of potency by 1,4-naphthoquinone, 5-hydroxy-1,4-napthoquinone (juglone) and 1 ...	1991	1687553
biochemical characterisation of the enzyme responsible for "activated l-serine sulphydrase" activity in nematodes.	the biochemical properties of the enzyme responsible for nematode "activated l-serine sulphydrase" activity (l-cysteine + r-sh----cysteine thioether + h2s) have been investigated using primarily the gastro-intestinal nematodes nippostrongylus brasiliensis and haemonchus contortus. the activated l-serine sulphydrase enzyme was found to be cytosolic in origin and exhibited maximal activity at ph 9.0. enzyme activity was widely distributed amongst the major tissues of adult female ascaris suum but ...	1992	1740180
isoenzymatic pattern and structure of glutamate dehydrogenase from ascaris suum.	the isoenzymatic pattern of glutamate dehydrogenase (gdh) has been described for ascaris suum a parasite of sus scrofa domestica. only one band of activity has been revealed, suggesting a monomorphic condition for this enzyme. also, the structure of gdh has been assayed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) and silver staining. only one subunit was present with a molecular weight of about 55,000. a hexameric structure for gdh of a. suum is suggested.	1992	1293199
antigen-induced late-phase airway obstruction in the guinea pig?	hartley or dunkin-hartley guinea pigs (n = 136) were actively sensitized to ovalbumin or ascaris suum protein by five different regimes. specific airway resistance (sr(aw)) was measured in conscious animals by a plethysmographic procedure before, immediately following and at various intervals (up to 96 h) after aerosolized antigen or vehicle challenge. each sensitization and challenge regime produced an immediate allergic response with positive responses (defined as a 2-fold increase in sr(aw)) ...	1992	1295358
lack of late-phase airway responses in conscious guinea pigs after a variety of antigen challenges.	guinea pigs were actively sensitized by parenteral injections of ovalbumin (oa), house dust extract (hd) or ascaris suum extract (as) in a variety of multidose regimens. at least 3 weeks after the initial sensitization injection, aerosols of the appropriate antigen were administered to conscious guinea pigs in a double-chamber body plethysmograph. oa elicited the most consistent and intense bronchoconstriction (bc) as measured by decreases in specific airway conductance (sgaw). the airway respon ...	1992	1295368
immunocytochemical and electrophysiological investigations of central neurones in the parasitic nematode ascaris suum.	here we report on preliminary investigations into the morphological, neurochemical and electrophysiological characteristics of central neurones in the parasitic nematode ascaris suum. the neurones do not display all or none action potentials and apparently have low resting membrane potentials. neurones have been shown to respond to acetylcholine. immunoreactivity to a diverse range of neuropeptides has been demonstrated.	1992	1299113
standardization of dot-elisa for the serological diagnosis of toxocariasis and comparison of the assay with elisa.	the dot-enzyme-linked immunosorbent assay (dot-elisa) was standardized using somatic (s) and excretory-secretory (es) antigens of toxocara-canis for the detection of specific antibodies in 22 serum samples from children aged 1 to 15 years, with clinical signs of toxocariasis. fourteen serum samples from apparently normal individuals and 28 sera from patients with other pathologies were used as controls. all samples were used before and after absorption with ascaris suum extract. when the results ...	1992	1307410
characterization of the major phosphofructokinase-dephosphorylating protein phosphatases from ascaris suum muscle.	in contrast to the mammalian enzyme, pfk from the nematode ascaris suum is activated following phosphorylation (daum et al. (1986) biochem. biophys. res. commun. 139, 215-221) catalyzed by a camp-dependent protein kinase (thalhofer et al. (1988) j. biol. chem. 263, 952-957). in the present report, we describe the characterization of the major pfk dephosphorylating phosphatases from ascaris muscle. two of these phosphatases exhibit apparent m(r) values of 174,000 and 126,000, respectively, and ar ...	1992	1321672
extracellular recordings from the motor nervous system of the nematode, ascaris suum.	1. the close association of muscle and neurons in ascaris suum makes it difficult to determine whether spikes recorded from nerve cords originate in muscle or neurons. we have developed criteria that distinguish muscle and neuronal activity. there are two categories of extracellular spikes. 2. the first category consists of spikes with a wide range of amplitudes, marked by large spikes. these spikes, which can be recorded over lateral muscle and over the dorsal and ventral nerve cords, are aboli ...	1992	1328624