| [the dynamics of important helminths in pig husbandry]. | the growth and seasonal dynamics of three helminth species (ascaris suum, oesophagostomum dentatum, and trichocephalus suis) were studied on a modern pig-fattening farm of the pm-016-agp type. the study of the dynamics of the incidence of the eggs of these helminth species demonstrated that large swine roundworm (ascaris suum) had favourable conditions for further development at average daily temperatures above 12 degrees c. in winter when the average daily temperatures were lower than 10 degree ... | 1980 | 6769208 |
| [the effect of technological and zoo-hygienic factors on the occurrence of helminths in different strains of pigs]. | seven helminth species were found in 14 different pig stocks in the south bohemian region. these were: ascaris suum, oesophagostomum dentatum, trichocephalus suis, hyostrongylus rubidus, strongyloides ransomi, capillaria sp. and metastrongylus sp. o. dentatum dominates in adult animals. a. suum, t. suis and s. ransomi are more frequently encountered in piglets and gilts. the primiparous sows can be therefore regarded as the main source of invasion for piglets. the composition of the animals' hel ... | 1980 | 6773221 |
| intestinal basement membrane of ascaris suum. physical properties of the collagenous domain. | | 1981 | 6783646 |
| activity of enzymes regulating glycogen metabolism in perfused muscle-cuticle sections of ascaris suum (nematoda). | a new perfusion system has been developed in which muscle-cuticle sections of ascaris suum were perfused, enabling study of enzymes in vitro. using this technique the activity of the regulatory enzymes glycogen synthase and glycogen phosphorylase was determined, and the level of glycogen in the muscle was assessed. during starvation, 98% of glycogen synthase was in the inactive d-form, and 80% of the glycogen phosphorylase activity was in the active a-form. when the ascarid muscle section was pe ... | 1981 | 6790691 |
| glycogen metabolizing enzymes during starvation and feeding of ascaris suum maintained in a perfusion chamber. | the glycogen content of muscle was correlated with the activity of glycogen synthase and glycogen phosphorylase from the parasitic roundworm ascaris suum maintained in vitro. adult female worms were maintained in the laboratory in a perfusion system during periods of starvation and feeding. during starvation, the levels of glucogen decreased at a rate of 0.1 to 0.2 mumoles/min/g wet weight of muscle-cuticle. during this time, 95% of the glycogen synthase (e.c. 2.4.1.11) was in the active d-form, ... | 1981 | 6790695 |
| effect of pharmacologic agents on antigen-induced decreases in specific lung conductance in sheep. | the purpose of this investigation was to determine the primary mediators responsible for the decrease in specific lung conductance (sgl) after airway challenge with ascaris suum antigen in allergic sheep. on different occasions, separated by 10 to 14 days, pulmonary resistance and thoracic gas volume were measured in 5 sheep with ascaris suum hypersensitivity before and for 2 h after a standard inhalation challenge with this antigen. initially and at the end of the study, inhalation challenge de ... | 1981 | 6795979 |
| [eosinophilia in white mice in experimental toxocariasis and recurrent ascariasis]. | twenty-five white mice were experimentally invaded by the larvae of toxocara cati, and their blood count was followed. on the seventh day after invasion the number of eosinophil leucocytes started to increase and the maximum (28%) was reached on the twenty-first day. after 45 days the mice were newly invaded by eggs of ascaris suum. the number of eosinophil leucocytes increased again and reached its maximum on the fourteenth day (26%). the increased eosinophilia in mice was observed until the 20 ... | 1982 | 6812263 |
| [changes in the number of t and b lymphocytes in the blood of pigs after experimental ascaridiosis]. | the changes in the number of the t and b dependent cells in the peripheral blood were studied after experimental invasion of piglets (live weight 8-10 kg) with the eggs of ascaris suum (50 000 eggs per piglet). the detection of both cell populations by two rosette methods (e and eac) showed that their first rise set in on the third day from invasion. the maximum occurrence of the t and b cells was recorded 17 days from invasion; on the 20th day from invasion their number returned to the initial ... | 1982 | 6818751 |
| detection and characterization of polymers in cephalothin by passive cutaneous anaphylaxis in mice. | immunization of balb/c mice by repeated injections of cephalothin (cet) - ascaris suum extract conjugate resulted in formation of ige antibodies, which were able to sensitize syngeneic animals for passive cutaneous anaphylaxis (pca). by means of high-pressure liquid chromatography, a fraction with extremely high pca-eliciting activity, but without appreciable antimicrobial activity, was isolated from the cet preparation. physicochemical analyses of the fraction identified the major component of ... | 1983 | 6822439 |
| ultrastructure and function of oviduct-uterine junction in ascaris suum (nematoda). | cells comprising the oviduct-uterine junction of the female reproductive tract in ascaris suum were shown to have a distinctive ultrastructure. the free luminal surface of each epithelial cell was bordered by an electron-dense layer that measured 90 to 120 nm in thickness and could be removed by treatment with pronase. numerous membrane-bound, filament-containing bodies were present in the cytoplasm near the apical margin. these filaments, measuring 6.5 nm in diameter, coalesce (or condense) to ... | 1983 | 6827433 |
| modification of allergic bronchoconstriction by a calcium antagonist: mode of action. | because both degranulation of mast cells and contraction of airway smooth muscle are dependent upon the influx of calcium, a calcium blocking agent might modify allergic bronchoconstriction by at least these two mechanisms. we treated sheep allergic to ascaris suum antigen with the calcium antagonist verapamil prior to airway challenge with an aerosol of ascaris suum antigen and also investigated the response without pretreatment. aerosolized ascaris suum antigen increased mean pulmonary resista ... | 1983 | 6859650 |
| changes in the blood picture of white mice experimentally infected with various species of ascarids. | the blood picture was studied in white mice experimentally infected with ascaris suum, toxocara cati, and toxascaris leonina. in mice infected with a. suum, maximum number (17%) of eosinophiles occurred on day 28 p.i. in mice infected with t. cati, the eosinophilia increased already from the first day after infection, reaching the maximum (26%) on day 21 p.i. in mice infected with t. leonina, the eosinophilia increased from day 7 to day 28 p.i. and a slightly increased number of eosinophiles per ... | 1983 | 6862317 |
| ribonucleotide levels in six nematodes, a cestode and a trematode. | the levels of ribonucleotides in the nematodes haemonchus contortus, ostertagia circumcincta, trichostrongylus colubriformis, nippostrongylus brasiliensis, ascaris suum and stephanurus dentatus, the cestode moniezia expansa and the trematode fasciola hepatica were measured by high pressure liquid chromatography. the adenylate charge, atp and total adenine nucleotide levels were in general agreement with those determined enzymatically and with published data. udp and guanine nucleotide levels wer ... | 1983 | 6877282 |
| regulation of the ascaris suum pyruvate dehydrogenase complex by phosphorylation and dephosphorylation. | the pyruvate dehydrogenase complex isolated from 'anaerobic' mitochondria of ascaris suum has a subunit composition similar to complexes isolated from most other eukaryotic organisms and is regulated by phosphorylation and dephosphorylation. pyruvate dehydrogenase kinase activity is stimulated by nadh and a number of physiologically important acyl-coa intermediates and is inhibited by coa, propionate, tiglate and pyruvate. it is suggested that the elevated levels of pyruvate observed in the asca ... | 1983 | 6877284 |
| flubendazole: dose range and efficacy studies against common internal parasites of swine. | the anthelmintic spectrum of activity and efficacy of flubendazole, using pigs naturally infected with helminth parasites, were determined in 4 experiments, using 157 pigs. flubendazole (1.5 mg/kg of body weight) mixed in feed or top-dressed on feed for 5 consecutive days was highly effective as an anthelmintic. this dosage level indicated 100% efficacy against mature ascaris suum, oesophagostomum dentatum, trichuris suis, and metastrongylus apri. there was 88% efficacy against strongyloides ran ... | 1983 | 6881670 |
| enzyme polymorphism in ascaris suum (nematoda). | the potential of multilocus electrophoretic studies for providing insight into the population biology of parasitic organisms was studied using the swine parasite ascaris suum suum. thirty-eight loci encoding enzymatic or nonenzymatic proteins have been resolved in extracts of adult worms by starch-gel electrophoresis. a preliminary study of variation in ascaris from eastern iowa revealed an average heterozygosity of 6.6%. allele frequencies at six polymorphic loci were similar in males and femal ... | 1982 | 6889643 |
| development of immune responsiveness to ascaris suum antigens in pigs vaccinated with ultraviolet-attenuated eggs. | pigs fed ascaris suum eggs attenuated by short wave ultraviolet-radiation developed resistance to challenge infection. per os inoculation of pigs on three successive weeks with 10,000 eggs irradiated to total exposures of 150,100 and 75 microw-min/cm2, respectively, resulted in an 88% reduction in the number of larvae recovered from the lungs 7 days after challenge with 10,000 infective eggs. peripheral blood lymphocytes (pbl) from vaccinated pigs were specifically stimulated in vitro to incorpo ... | 1982 | 6889778 |
| ascaris suum: oxidative phosphorylation in mitochondria from developing eggs and adult muscle. | | 1982 | 6889977 |
| determination of the specific gravity of certain helminth eggs using sucrose density gradient centrifugation. | the specific gravities of ten species of helminth eggs were determined using sucrose density gradient centrifugation. fecal or egg concentrate was layered over a 3 to 54% sucrose density gradient. the gradient was then centrifuged at 800 g for 20 min, allowing 5 min for acceleration and 5 for deceleration. bands formed were identified and measured. refractive index was measured at the middle of narrow bands, or at the level at which the concentration of eggs was highest, in the case of wide band ... | 1982 | 6890102 |
| reversible inhibition of hatching of infective eggs of ascaris suum (nematoda). | | 1982 | 6890547 |
| experimentally induced resistance to ascaris suum in pigs. | the present study shows that oral inoculation with infective a. suum eggs or 3rd stage larvae in pigs aged 5-16 weeks induces resistance against oral challenge with infective a. suum eggs. acquired resistance is reflected by an adaptive type of host response as measured by increased pathological, serological and hematological responses and decreased recovery of worms from lungs and small intestine. although based on a limited number of experimental pigs, the results of the present experiments su ... | 1982 | 6890668 |
| survival rates of parasite eggs in sludge during aerobic and anaerobic digestion. | the effects of mesothermic anaerobic or aerobic sludge digestion on survival of eggs from the roundworms ascaris suum, toxocara canis, trichuris vulpis, and trichuris suis and from the rat tapeworm hymenolepis diminuta were studied. destruction of eggs throughout a 15-day treatment period, as well as their viabilities after reisolation, was analyzed. the laboratory model digesters used in this study were maintained at a 15-day retention schedule, partially simulating a continuously operating sys ... | 1982 | 6891199 |
| electrophoretic analyses of myofibrillar proteins from the body wall muscles of ascaris suum. | a myofibrillar protein extract has been isolated from the muscle of ascaris suum. two-dimensional electrophoresis of this extract revealed that the myosin light chain 1 (alc1) migrates as 3 components with approximate isoelectric points in the range of 5.3-5.6. the most acidic component of alc1 appeared to be phosphorylated when the myofibrillar extract was incubated for 10 s with catalytic subunit of camp dependent protein kinase and [gamma-32p] atp. the myosin light chain 2 (alc2) migrated as ... | 1981 | 6894780 |
| efficacy of ivermectin against five genera of swine nematodes and the hog louse, haematopinus suis. | twenty pigs sequentially infected with oesophagostomum spp, metastrongylus spp, and trichuris suis, and maintained on lots contaminated with ascaris suum and strongyloides ransomi, were assigned to 1 of 4 groups on the basis of weight, sex, and litter. each group of pigs was given ivermectin subcutaneously at the rate of 0, 20, 100, or 500 microgram/kg of body weight. lice on pigs were counted before treatment, on posttreatment day 1, and just before necropsy. pigs were necropsied on posttreatme ... | 1981 | 6895286 |
| ascaris suum: t-cell responses of c57bl/6j mice in vitro and in vivo. | | 1981 | 6970679 |
| ascaris oocytes: ultrastructural and immunocytochemical changes during passage through the oviduct. | slender, bipolar, immature, ascaris suum oocytes assume a smooth, spherical configuration as they traverse the oviduct. the present investigation was conducted to determine if this morphological alteration might be accompanied by changes in surface membranes. immunocytochemical studies were conducted utilizing (1) fluorescein-labeled, sheep, anti-rabbit igg, (2) sternberger's unlabeled, antibody-enzyme technique, (3) ferritin-labeled, sheep, anti-rabbit igg, and (4) an immunobead, labeled-antibo ... | 1980 | 6993646 |
| diagnosis of human strongyloidiasis by immunofluorescence, using strongyloides ratti and s. stercoralis larvae. | the sensitivity and specificity of an indirect immunofluorescent antibody assay for the diagnosis of human strongyloidiasis has been investigated. sera were obtained from 160 australian ex-servicemen who had been prisoners-of-war in southeast asia during world war ii, 44 of whom proven parasitologically to have strongyloidiasis; these men did not have concurrent infections with other helminths. in addition, sera were collected from 44 age- and sex-matched australians who were not thought to have ... | 1981 | 7015889 |
| [serological differentiation of ascaris suum and toxocara canis infections in pigs (author's transl)]. | groups of eight miniature pigs were infected either with 1,500 embryonated eggs each of ascaris suum or toxocara canis or with 1,000 eggs each of both nematodes. sera were sampled before the infection as well as three and six weeks postinfection and then investigated in the elisa on microtitre plates against antigens of various developmental stages. when extracts of adult ascaris and toxocara worms were used as antigens, distinct reactions were registered both in the infected and in the uninfect ... | 1981 | 7032103 |
| serodiagnosis of human strongyloidiasis by an enzyme-linked immunosorbent assay. | the sensitivity specificity of an enzyme-linked immunosorbent assay (elisa) for the serodiagnosis of strongyloidiasis has been investigated. 45 men with long-standing strongyloidiasis were compared with the same number of age- and sex-matched control subjects. the elisa detected antibody in 84% of patients with parasitologically proven strongyloidiasis. when the technique was compared with an indirect immunofluorescent assay (ifa), a high correlation coefficient was obtained. specificity was dem ... | 1981 | 7036430 |
| [serological demonstration of experimental round worm infections-ascaris suum, toxocara canis--in swine by means of the indirect immunofluorescence antibody test]. | by means of indirect immunofluorescent antibody reaction (ifar), using serum of experimentally infected pigs, various antigens were studied with regard to their usefulness for serological verification of prepatent ascaris suum and toxocara canis infections. eggs, egg larvae, larvae received from livers, lungs and brains of experimentally infected white mice and sections of adult t. canis as well as eggs, egg larvae, liver larvae and sections of frozen adult a. suum proved to be not suitable for ... | 1981 | 7039425 |
| evaluation of three immunodiagnostic procedures for porcine strongyloidiasis. | an indirect fluorescent antibody test (ifat), an indirect radioimmune antibody test (irat), and an enzyme-linked immunosorbent antibody assay (elisa) were compared for their efficacies in detecting antibody to strongyloides ransomi in pigs. the greatest sensitivity was exhibited by the elisa, using a soluble, whole larval extract. the 2 indirect antibody assays, ifat and irat, using intact 3rd-stage larvae (l3) were comparable in sensitivities. the use of live l3 in the ifat and irat presented t ... | 1982 | 7041716 |
| genetic control of eosinophilia in mice: gene(s) expressed in bone marrow-derived cells control high responsiveness. | a heterogeneity in the capacity of strains of mice to mount eosinophilia is described. balb/c and c3h are eosinophil high responder strains (eo-hr) and cba and a/j are eosinophil low responder strains (eo-lr), judged by the response of blood eosinophils to ascaris suum, and the response of blood, bone marrow, and spleen eosinophils to keyhole limpet hemocyanin given 2 days after 150 mg/kg cyclophosphamide. some of the gene(s) for high responsiveness appear to be dominant because (eo-hr x eo-lr)f ... | 1982 | 7054293 |
| purification and properties of phosphoenolpyruvate carboxykinase from ascaris suum. | | 1982 | 7076387 |
| [the larva precipitation test with ascaris suum and toxocara canis larvae]. | investigations were made by means of a larvae precipitation test (lpt) on different laboratory animals (pigs and white mice, and pigs, rabbits, guinea pigs and white mice respectively) experimentally infected with ascaris suum and toxocara canis respectively for the serological evidence of specific a. suum and t. canis antibodies. living a. suum larvae isolated from the lungs of experimentally infected white mice forming oral, excretion porus and cuticula precipitates proved to be a suitable ant ... | 1982 | 7081762 |
| role of ciliary motility in acute allergic mucociliary dysfunction. | the purpose of this investigation was to determine if abnormal ciliary function contributes to allergic mucociliary dysfunction. in conscious sheep with ascaris suum hypersensitivity, ciliated cells were obtained with a cytology brush and tracheal mucous velocity (tmv) was determined before and serially for 2 h following antigen inhalation. the recovered cells (also containing mast cells) were suspended in a chamber, and ciliary activity was viewed microscopically and recorded on videotape for s ... | 1982 | 7085401 |
| [protective properties of the cavitary fluid antigens of ascaris suum]. | fractions of cavitary fluid of ascaris (cfa) containing antigens were obtained on membranous filters. being administered to mice they provide the development of protective immunity of animals to their subsequent infection with invasional eggs of a. suum. in mice immunized with fraction cfa xm-50 the number of a. suum larvae obtained from the lungs on the 7th day of invasion was confidentially lower than in control animals (15.277) +/- 1.825 on the 7th day of invasion was confidentially lower tha ... | 1982 | 7122119 |
| mitochondrial nadh oxidase activity of adult hymenolepis diminuta (cestoda). | 1. mitochondria from adult hymenolepis diminuta displayed a membrane-associated, rotenone-sensitive nadh oxidase and nadh-dependent fumarate reductase system. 2. both the h. diminuta oxidase and fumarate reductase were relatively insensitive to antimycin a. potassium cyanide and sodium azide, at concentrations which significantly inhibited the nadh oxidase system of rat liver. 3. malonate effectively depressed the mitochondrial nadh oxidase activity of both h. diminuta and adult ascaris suum (ne ... | 1982 | 7128112 |
| ascaris suum: differential effects of avermectin b1a on the intact animal and neuromuscular strip preparations. | | 1982 | 7128715 |
| studies on chemotherapy of parasitic helminths (vii). effects of various cholinergic agents on the motility of angiostrongylus cantonensis. | effects of various cholinergic agents on the motility of angiostrongylus cantonensis were studied to define the neuropharmacological properties of this worm. stimulation of the motility and/or contraction were shown by eserine, ach, carbachol, nicotine, dmpp, pyrantel, and ba2+, but not by pilocarpine and mcn-a-343. contraction was similarly observed by these agents in the preparations paralyzed with praziquantel. paralysis was caused remarkably by d-tubocurarine and slightly by succinylcholine, ... | 1982 | 7131950 |
| a radioallergosorbent test (rast) for ascaris lumbricoides. | an antigen from ascaris lumbricoides obtained from infected humans has been prepared which can be used for detecting immune response to ascaris using the skin-prick test. a comparison of immune skin responses between this antigen and the commercial (bencard) ascaris suum antigen has been made and a rast technique for the in vitro detection of specific ige to ascaris lumbricoides has been developed using the lumbricoides antigen. a correlation between skin-prick test and rast determinations and b ... | 1982 | 7137527 |
| electrophysiological effects of piperazine and diethylcarbamazine on ascaris suum somatic muscle. | 1 electrophysiological recordings were made from the bag region of ascaris suum muscle. membrane potential and input conductance or membrane current under voltage clamp were measured. 2 in high-cl- ringer, bath-applied piperazine, at concentrations greater than 10(-4)m, produced a dose-dependent and reversible increase in input conductance associated with a hyperpolarizing potential. the increase in input conductance was reduced when the preparations were bathed in low-cl- ringer. gamma-aminobut ... | 1982 | 7139188 |
| cross resistance between ascaris suum and nippostrongylus brasiliensis in mice. | the present study shows that n. brasiliensis infection in thymus-bearing mice results in resistance against a subsequent a. suum infection. in contrast thymus-deficient mice do not develop this heterologous resistance. thymus-deficient mice, on the other hand, develop resistance to homologous challenge although to a lesser degree than thymus-bearing mice. further, it is shown that a. suum infection in thymus-bearing mice yields resistance against a subsequent n. brasiliensis infection. the under ... | 1982 | 7145653 |
| hexokinase from ascaris suum muscle. | | 1982 | 7154947 |
| fructose-bisphosphate aldolase from ascaris suum. | | 1982 | 7154952 |
| migration of some roundworm species in experimentally infected white mice. | the migration of four roundworm species (ascaris suum, toxocara canis, t. cati and toxascaris leonina) was studied in various organs of experimentally infected white mice. the hatching of t. leonina and t. cati larvae in the paratenic host is more rapid (24-36 h) than the hatching of a. suum and t. canis larvae (1-6 days). the deposition of larvae in host organs is different in the individual species. maximum number of larvae in the liver: t. leonina and a. suum on day 4, t. canis on day 2 and t ... | 1982 | 7160786 |
| inhibition of human blood clotting by extracts of ascaris suum. | the effects of soluble extracts of ascaris suum on human blood coagulation were investigated. whole worm supernatant solution prolonged the whole blood clotting time and the kaolin-activated, partial thromboplastin time but it did not alter the prothrombin time. these data indicate impairment of the intrinsic pathway of blood coagulation. whole worm supernate inhibited platelet aggregation induced by adp and ristocetin. this supernate did not affect fibrinolysis. the maximal concentration of ant ... | 1982 | 7175610 |
| egg-shell permeability and hatching of ascaris suum. | when eggs of ascaris suum were transferred from glass-distilled water into 0.1--0.4 m nacl solutions, the water content of the unhatched juveniles fell with increasing concentration of solute. the effect was reversible. the egg-shell was thus permeable to water. the osmotic pressure of the egg fluid was osmotically equivalent to between 0.1 and 0.2 m trehalose. in hatching experiments in fairbairn's medium containing 0.1 or 0.2 m trehalose, only 5 and 3% respectively of the eggs hatched; 83% of ... | 1980 | 7190259 |
| prophylactic medication with pyrantel to prevent liver condemnation in pigs naturally exposed to ascaris infections. | the use of pyrantel tartrate (106 mg/kg of feed) as a continuous feed medication was evaluated in 848 finishing hogs for efficacy in preventing ascaris suum infections, and in reducing liver fibrosis and liver condemnation at slaughter, associated with a suum infections. liver condemnations due to ascaris damage were reduced 100% in all pigs given pyrantel, when compared with condemnation in non-medicated controls. none of the livers from any of the medicated animals was condemned, whereas liver ... | 1980 | 7194010 |
| the involvement of sugars and mg++ in the attachment of phagocytic cells to ascaris suum juveniles. | in vitro adherence of washed peritoneal exudate cells (pecs) from mice to ascaris suum juveniles was inhibited by d-glucosamine and d-galactosamine. other carbohydrates tested had no apparent effect on adherence after incubation for 3 hr at any of the concentrations tested. furthermore, attachment of pecs to a. suum juveniles was completely inhibited by the addition of ethylenediaminetetraacetate to the medium, but magnesium ethylene glycol bis(trichloroacetate) had no effect on adherence. the r ... | 1981 | 7217714 |
| impaired tracheal mucus transport in allergic bronchoconstriction: effect of terbutaline pretreatment. | the transport velocity of mucus within the trachea, pulmonary resistance, and arterial blood gas composition were measured in intubated conscious sheep with ascaris suum sensitivity before and during allergic bronchoconstriction. inhalation of a. suum extract for 15 min increased mean pulmonary resistance significantly from 1.9 cm h2o . l-1 sec-1 to 5.1 cm h2o . l-1 sec-1 after 60 min and to 4.5 cm h2o . l-1 sec-1 after 120 min, while it decreased mean arterial po2 from 85 to 59 and 53 mm hg, re ... | 1981 | 7229224 |
| anthelmintic activity of oxfendazole in pigs. | eighty-five pigs were artificially infected with hyostrongylus rubidus, oesophagostomum species and ascaris suum. on days 2, 10, 20 or 51 after infection groups of six were treated with oxfendazole premix in food at various dose rates. subsequently the pigs were slaughtered for comparative worm counts to be made in treated and control animals. the efficacy of treatment against hyostrongylus worms of increasing age was 68, 57, 99.8 and 100 per cent after a 3 mg per kg dose, 75, 75, 99.8 and 100 p ... | 1981 | 7233774 |
| purification of malic enzyme from ascaris suum using nad+-agarose. | malic enzyme has been purified from ascaris suum by polyethylene glycol precipitation, ion-exchange chromatography, ammonium sulfate precipitation, and nad-agarose affinity chromatography to a specific activity of 80 units/mg (v/[e]t = 350 s-1). the preparation was shown to be homogeneous by sds polyacrylamide disc gel electrophoresis. the procedure can be accomplished in a maximum of four days with a 74% yield. | 1981 | 7242572 |
| bone marrow cells associated with heightened eosinophilopoiesis: an electron microscope study of murine bone marrow stimulated by ascaris suum. | an acute eosinophilopoiesis occurs in mice secondarily exposed to ascaris suum, the marrow eosinophils increasing from approximately 5% to 45%, and blood levels from 50/ml3 to more than 1000/ml3, within 14 days. eosinophilopoiesis occurs in hematopoietic compartments of the bone marrow and is associated with four other cell types: branched stromal cells, macrophages, lymphocytes, and reticular cells adventitial to venous sinuses. branched stromal cells, a newly recognized cell type, are presente ... | 1981 | 7246451 |
| a survey of gastrointestinal parasitism in pigs. | over a period of two years 180 pork pigs, 144 baconers and 144 sows from two slaughterhouses in northern england were examined for gastrointestinal parasites. sows showed the highest level and intensity of infection, 85 per cent harbouring the dominant parasites oesophagostomum dentatum and o quadrispinulatum; the highest worm burden recorded was 21,000. hyostrongylus rubidus was present in 28.5 per cent of sows but virtually absent from younger pigs. small numbers of ascaris suum and trichuris ... | 1980 | 7257108 |
| a case of ascaris suum infection in lambs. | | 1980 | 7257116 |
| indirect solid-phase microradioimmunoassay for detection of ascaris suum antibodies in swine sera. | antibodies to ascaris suum have been detected and quantitated in sera of swine orally inoculated with eggs of a suum, using an indirect solid-phase microradioimmunoassay. antibody titer of 1:640 to 1:10,240 were quantitated, using purified and crude ascarid antigens in the assays. swine sera from 71 of 72 farm herds located in 37 counties in nebraska had at least 1 serum of the 7 tested with a titer equal to or greater than 1:20. a total of 437 sera were positive and 67 negative (ie, less than 1 ... | 1981 | 7258808 |
| efficacy of fenbendazole against five genera of swine parasites. | thirty-three pigs were sequentially infected with oesophagostomum spp, metastrongylus spp, and trichuris suis while being maintained in lots contaminated with space ascaris suum, strongyloides ransomi, and other parasites. pigs were assigned to 1 of 3 groups on the basis of weight (6.8 to 25.9 kg), sex, and litter. pigs in the 1st group were used as nontreated controls; pigs in the 2nd and 3rd groups were given fenbendazole in the feed at doses of 3 and 5 mg/kg of body weight, respectively, on 3 ... | 1981 | 7271035 |
| the basenji-greyhound dog: antigen-induced changes in lung volumes. | subdivisions of lung volume, closing volume (cv), pulmonary resistance (rl) and dynamic pulmonary compliance (cdyn) were measured before and after bronchial challenge with ascaris suum in seven ascaris-sensitive basenji-greyhound (bg) crossbreed dogs. rl increased from a control value of 1.41 +/- 0.43 (mean +/- se) cm h2o/l/sec to 21.4 +/- 5.15 (p less than 0.01) and cdyn decreased from 163 +/- 32 ml/cm h2o to 43 +/- 11 (p less than 0.01) in the first 15 min following ascaris-antigen aerosol (aa ... | 1981 | 7280384 |
| effects of atmospheric ammonia on young pigs experimentally infected with ascaris suum. | effects of atmospheric ammonia at 69.4 mg/m3 (100 ppm) on productive performance and respiratory tract health of young pigs (starting body weight averaged 7.5 kg) experimentally infected with ascaris suum (50,000 embryonated ova administered by gavage when pigs were 5 weeks of age) were studied in 5 trials of 4 weeks each (when pigs were 5 to 9 weeks of age). effects of atmospheric-ammonia exposure and ascarid infection on growth were additive. compared with controls, percentage reductions in av ... | 1981 | 7283247 |
| pathogenesis of liver lesions in mice following a primary infection with ascaris suum. | the development of liver lesions in mice infected with ascaris suum is described and compared to the lesions in pigs. the findings suggest that liver necroses in mice are initially periacinar (centrilobular), caused by anoxia resulting from a vascular obstruction by the a. suum larvae located in the sinusoids; and that the lesions in pigs following a primary infection have a different pathogenesis. it is concluded that mice are unsuitable models for studying the pathogenesis of a. suum-induced l ... | 1981 | 7292896 |
| immunopathology of ascarid infection of the eye. role of ige antibodies and mast cells. | the roles of ige antiascarid antibodies and mast cells were compared in passively sensitized guinea pigs and animals infected intravitreally with ascarid larvae (toxocara canis, ascaris suum). intravenous ige antibody disappeared from the serum within 48 hours, but induced a hypersensitive state that persisted for 28 days. in systemically immunized animals, the aqueous-serum ige antibody ratio was 1:1,000 or less. passive periocular anaphylactic reactions produced an infiltration of neutrophils ... | 1981 | 7295137 |
| accessory nuclei in female ascaris suum. | accessory nuclei were observed in early meiotic prophase (leptotene) in female ascaris suum but such structures were absent in meiosis in males. accessory nuclei also were observed during the prediminution embryonic divisions. all embryos did not have accessory nuclei, thus, those embryos with these structures may be female since the accessory nuclei are maternal-limited. accessory nuclei were not observed after the fourth embryonic division, at which stage the process of chromatin diminution be ... | 1981 | 7299580 |
| subcellular fractions and the refringent granules of the spermatozoa of ascaris suum (nematoda). | six subcellular fractions were isolated by differential centrifugation of the homogenate of spermatozoa of ascaris suum. the cellular constituents of pelleted fractions, as identified by electron microscopy, were membranes and membranous organelles (fraction a1), microsomal (a2), cytoplasmic (a3), large refringent granules (b1), small refringent granules (b2) and a detergent-soluble fraction (b3). polypeptide analysis of sds-page showed that the 18,400-dalton band, one of the major spermatozoan ... | 1981 | 7317939 |
| differences in airway reactivity in normal and allergic sheep after exposure to sulfur dioxide. | the effect of breathing 5 ppm sulfur dioxide (so2) on airway reactivity was studied in both normal and allergic conscious sheep. allergic sheep were defined as animals in which inhalation of ascaris suum extract resulted in bronchospasm as evidenced by an increase in mean pulmonary flow resistance (rl), hyperinflation, and a fall in dynamic compliance. airway reactivity was assessed by measuring the increase of rl after 18 breaths of 0.25% carbachol (c), from an initial rl value obtained after 1 ... | 1981 | 7319895 |
| [the analysis of immunologically similar substance with ascaris suum specific protein in rat colon mucosa (author's transl)]. | | 1981 | 7321258 |
| [effect of glycerin, paraffin, rapeseed oil and aqueous solutions on the development of ova of ascaris suum goeze, 1782]. | | 1981 | 7324462 |
| in vitro development of ascaris suum from third- to fourth-stage larvae and detection of metabolic antigens in multi-well culture systems. | a population of early to late third-stage larvae (l3) of ascaris suum obtained from the lungs of swine 7 days after infection developed to the fourth stage (l4) in stationary, multi-well, culture plates in an atmosphere of 5% co2 in air. larval survival, growth and morphogenesis were evaluated in five culture systems consisting of dulbecco's modified eagles' medium alone (dm) or containing a serum supplement (dm-s) or a tripeptide (1-glycyl-1-histidyl-1-lysine) at final concentrations of 20, 200 ... | 1981 | 7328453 |
| coupling of mitochondrial nadph : nad transhydrogenase with electron transport in adult hymenolepis diminuta. | the mitochondrial electron transport system of adult hymenolepis diminuta exhibited an apparent specificity in terms of reduced pyridine nucleotide utilization. the preferred substrate for both the minor oxidase and the physiologically required fumarate reductase system was nadh. intramitochondrial reducing equivalents, needed for phosphorylation via the anaerobic, electron transport-dependent, fumarate reductase, were generated as nadph by the action of the cestode's nadp-specific "malic" enzym ... | 1981 | 7328455 |
| intestinal tubulin as possible target for the chemotherapeutic action of mebendazole in parasitic nematodes. | in vitro incubation of the parasitic nematode ascaris suum in the presence of 10 microm mebendazole (mbz) resulted in a complete loss of colchicine binding ability of extracts obtained from the parasite's intestine. biochemical evidence supported the identification of the colchicine binding receptor in a. suum intestinal extracts as tubulin. this protein was partially purified and found to comprise approximately 0.8% of the soluble intestinal protein. mbz inhibited colchicine binding to the part ... | 1981 | 7335116 |
| sulfur dioxide induced airway hyperreactivity in allergic sheep. | the effects of a four-hour exposure (via a plexiglas hood) to sulfur dioxide (so2) on airway reactivity was studied in both normal and allergic conscious sheep. allergic sheep were defined as animals in whom inhalation of ascaris suum extract resulted in an increase in mean pulmonary flow resistance (rl). airway reactivity (delta rl) was assessed by measuring the increase in rl after 18 breaths of 0.25% carbachol, from an initial value obtained after 18 breaths of buffered saline. rl and delta r ... | 1980 | 7342777 |
| ascaris suum: nutrient absorption, growth, and intestinal pathology in young pigs experimentally infected with 15-day-old larvae. | | 1980 | 7350001 |
| pharmacological characteristics of cholinoreception in somatic muscles of the nematode, ascaris suum. | | 1980 | 7364197 |
| tubulin characterization during embryogenesis of ascaris suum. | | 1980 | 7380098 |
| interaction of anthelmintic benzimidazoles with ascaris suum embryonic tubulin. | the ability of mebendazole and fenbendazole to bind to tubulin in cytosolic fractions from 8-day ascaris suum embryos was determined by inhibition studies with [3h]colchicine. colchicine binding in the presence of 1 . 10(-6) m mebendazole was completely inhibited during a 6 h incubation period at 37 degrees c. inhibition of colchicine binding to a. suum embryonic tubulin by mebendazole and fenbendazole appeared to be noncompetitive. the inhibition constants of mebendazole and fenbendazole for a. ... | 1980 | 7388055 |
| potentiation of the reaginic (ige) antibody response to ovalbumin in the guinea pig with a soluble metabolic product from ascaris suum. | a soluble antigen, the acf antigen, obtained from cultures of larvae of ascaris suum was able to potentiate the reaginic response to an unrelated antigen was administered at the same time or 4 days after ovalbumin, there was a weak primary response and a strong statistically significant secondary ige response, compared with animals immunized only with ovalbumin. this potentiated response was not observed when the acf antigen was presented 10 days before or 8 or 12 days after the ovalbumin. | 1980 | 7391579 |
| iodination of surface components of the spheroidal and ameboid spermatozoa of ascaris suum (nematoda). | | 1980 | 7397296 |
| effect of local antigen inhalation and hypoxia on lobar blood flow in allergic dogs. | to determine the local pulmonary vascular response to inhaled antigen and alveolar hypoxia in canine asthma, 8 of 42 skin-test-positive dogs were selected from a preliminary study of airway reactivity to antigen challenge with an extract of ascaris suum. in an anesthetized, open-chest preparation subjected to left cervical vagotomy, the proportion of pulmonary blood flow to the left lower lobe (q lobe/q lung) was estimated by an insoluble gas-elimination method. continuous inhalation of antigen ... | 1980 | 7406342 |
| chromosome diminution in ascaris suum. chromatin structure. | | 1980 | 7408987 |
| [action of cholinolytic substances on the somatic musculature of the nematode, ascaris suum]. | | 1980 | 7421755 |
| experimental ascaris suum infection in pigs. serological response, eosinophilia in peripheral blood, occurrence of white spots in the liver and worm recovery from the intestine. | experimental oral inoculation of pigs with infective a. suum eggs resulted in eosinophilia in the peripheral blood and a serum antibody response. when pigs were inoculated at 3 days of age the antibody response was depressed which is suggested to be due either to a protective effect of colostral antibodies or to an immunologic incompetence of the young pigs. despite the use of isolated pens and strict hygienic measures it was difficult to obtain an ascaris free environment for the experimental a ... | 1980 | 7433010 |
| basenji-greyhound dog model of asthma: reactivity to ascaris suum, citric acid, and methacholine. | bronchial inhalation challenges to ascaris suum, citric acid, and methacholine were performed in eight basenji-greyhound (bg) crossbreed dogs, five of which were reactive to ascaris antigen aerosols (aaa). responses to aerosol challenges were measured as changes in pulmonary resistance (rl) and dynamic compliance (cdyn) and were compared to responses obtained in five mongrel dogs. responses to methacholine and citric acid were similar in bg dogs whether ascaris-sensitive or not, but bg dogs were ... | 1980 | 7440303 |
| [allergen-induced airway obstruction and histamine concentration in arterial and venous plasma. first communication (author's transl)]. | in 19 dogs sensitized against ascaris suum extract we measured dynamic elastance, arterial plasma histamine concentration, pressure in the a. femoralis, and arterial blood gases. in six dogs the venous plasma histamine concentration was also analyzed. ascaris extract (a.e.) inhalation increased both the arterial and the venous plasma histamine concentration significantly. the venous concentration was 61% of that detected in the arterial blood. at the same arterial plasma histamine level a large ... | 1980 | 7444174 |
| comparison of the responsiveness to histamine and to ascaris suum challenge in dogs. | the pulmonary and dermal sensitivity of a group of mongrel dogs to an extract of ascaris suum protein was compared with the pulmonary and dermal sensitivity to histamine in this same group. we found a modest but significant correlation in the entire population between skin test reaction to histamine and to a. suum protein (r = 0.52, p < 0.05), but when the skin test results were compared with those of aerosol challenge, no significant correlation was found. however, when those dogs with reaction ... | 1980 | 7447158 |
| suppression of reaginic antibodies with modified allergens. iii. preparation of tolerogenic conjugates of common allergens with monomethoxypolyethylene glycols of different molecular weights by the mixed anhydride method. | our previous findings that antigens, such as ovalbumin (oa) and the extract of ragweed pollen (rag), could be rendered nonantigenic, nonallergenic and tolerogenic by conjugation with polyethylene glycol (peg) have been extended in the present study to the synthesis of conjugates of a variety of antigens with monofunctional monomethoxy-pegs (mpegs) of different molecular weights by the use of the mixed anhydride method. thus, mpegs with molecular weights of 2,000, 5,000, 10,000 and 20,000 were co ... | 1981 | 7450906 |
| homology between roundworm (ascaris) and hookworm (n. americanus) antigens detected by human ige antibodies. | utilizing the techniques of radioallergosorbent test (rast), inhibition of rast and isoelectric focusing on polyacrylamide gels, antigenic cross-reactivity has been shown between extracts of ascaris lumbricoides. ascaris suum and necator americanus (hookworm). antigenic homology is extensive between the two ascaris preparations. the antigens common to hookworm and ascaris, while representing a minor component of ascaris body fluid, are nonetheless potent stimulators of ige antibody production in ... | 1980 | 7458774 |
| ascaris suum in saskatchewan pigs: an abattoir survey of prevalence and intensity of infection. | in a survey of 2500 market weight pigs in a saskatchewan abattoir, 37% were infected with adult ascaris suum and in 46% there were milkspot hepatic lesions. a total of 60% of the pigs examined had some evidence of a. suum infection. most infected pigs contained less than 50 adult ascarids. in a second abattoir survey of a further 2500 market weight pigs, 44% of the animals had milk-spot hepatic lesions. in approximately 12% of the affected pigs these lesions were very severe and a very similar p ... | 1980 | 7459794 |
| evaluation of left ventricular diastolic function in ige-mediated anaphylaxis in dogs. | depressed left ventricular (lv) systolic function remains controversial in patients with anaphylaxis and anaphylactic animal models. however, lv diastolic function has not yet been investigated in either patients or animal models. therefore, we assessed lv diastolic and systolic function in ige-mediated anaphylaxis in dogs. seven dogs sensitized to ascaris suum antigen were studied. an aqueous extract of ascaris suum antigen was administered over 30 s into the systemic circulation to induce anap ... | 1995 | 7484036 |
| ability of histamine to increase nasal mucosal permeability to macromolecules in guinea pigs. | the effect of histamine on nasal mucosal permeability against an antigen was investigated by using modified passive cutaneous anaphylaxis (pca) reactions in normal and actively sensitized guinea pigs. the administration of a dinitrophenyl-coupled ascaris (dnp-ascaris) solution as an antigen into the nasal cavity caused pca reactions in the dorsal skin of normal guinea pigs. the administration of histamine into the nasal cavity before the antigen treatment significantly enhanced the anaphylactic ... | 1995 | 7492070 |
| antigen (dnp-as)-induced allergic rhinitis model in guinea pigs. | in guinea pigs, an ige antibody was produced by intraperitoneal injection of antigen (dnp-as) containing al(oh)3 and booster inhalation of the antigen into the nasal cavity. experimental allergic rhinitis was induced by the perfusion of antigen solution into the nasal cavities of actively sensitized guinea pigs. severity of allergic rhinitis was assessed by determining release of histamine and leakage of dye into the nasal cavity. the antigen-induced release of histamine was significantly increa ... | 1994 | 7510462 |
| immune responses to nematode exoantigens: sensitizing antibodies and basophil histamine release. | high levels of ige and eosinophilia are found in both allergy and helminth infections, but allergic symptoms are rare in naturally acquired helminth infections. the interrelation of specific ige antibodies and in vitro basophil histamine release (hr) induced by exoantigens from the larval stages (l2/l3) of the nematodes toxocara canis and ascaris suum was examined in 148 patients visiting an outpatient clinic for parasitic diseases. the antigen sensitivity of the basophils was found to be depend ... | 1994 | 7521141 |
| adhesion molecules in acute and chronic lung inflammation. | | 1993 | 7524284 |
| the mitochondrial ribosomal rna genes of the nematodes caenorhabditis elegans and ascaris suum: consensus secondary-structure models and conserved nucleotide sets for phylogenetic analysis. | the small- and large-subunit mitochondrial ribosomal rna genes (mt-s-rrna and mt-l-rrna) of the nematode worms caenorhabditis elegans and ascaris suum encode the smallest rrnas so far reported for metazoa. these size reductions correlate with the previously described, smaller, structurally anomalous mt-trnas of c. elegans and a. suum. using primer extension analysis, the 5' end nucleotides of the mt-s-rrna and mt-l-rrna genes were determined to be adjacent to the 3' end nucleotides of the trna(g ... | 1994 | 7528811 |
| the action of pyrantel as an agonist and an open channel blocker at acetylcholine receptors in isolated ascaris suum muscle vesicles. | the anthelmintic pyrantel is believed to act as an agonist at acetylcholine receptors on somatic muscle from the parasite ascaris suum. this study aimed to confirm this mode of action of pyrantel. single-channel recordings from muscle vesicles formed from the extrasynaptic region of the bag of somatic muscle cells of ascaris suum were made using the patch-clamp technique. pyrantel (0.03-100 microm) activated cation-selective channels with at least 2 conductance levels: main conductance 41 +/- 2. ... | 1994 | 7535704 |
| oxantel-activated single channel currents in the muscle membrane of ascaris suum. | the patch clamp technique was used to investigate the action of the anthelmintic drug, oxantel, on nicotinic acetylcholine receptor (nachr) currents recorded from vesicles of the somatic muscle cells of the nematode parasite ascaris suum. the amplitudes of the currents were analysed at different membrane potentials to determine the single channel conductance. also the open and closed durations were measured to determine the kinetic properties of the activated channel. oxantel activated single na ... | 1995 | 7538657 |
| immuno-gold-labelling of cut-1, cut-2 and cuticlin epitopes in caenorhabditis elegans and heterorhabditis sp. processed by high pressure freezing and freeze-substitution. | cut-1 and cut-2 are two distinct protein components of cuticlin, the insoluble residue of the cuticles of nematodes. in previous experiments of gold-immuno-labelling on sections of chemically fixed caenorhabditis elegans, cut-1 and cut-2 epitopes were specifically lost. cryo-immobilization of c. elegans under high pressure followed by freeze-substitution, however, resulted in a good preservation of these antigenic sites and of the ultrastructure of the worms. the entomopathogenic nematode hetero ... | 1995 | 7545536 |
| caenorhabditis elegans ubc-1, a ubiquitin-conjugating enzyme homologous to yeast rad6/ubc2, contains a novel carboxy-terminal extension that is conserved in nematodes. | the rad6/ubc2 gene from saccharomyces cerevisiae encodes a ubiquitin-conjugating enzyme involved in dna repair, induced mutagenesis, and sporulation. here we report the isolation and characterization of the caenorhabditis elegans rad6 homolog designated ubc-1. ubc-1 encodes a 21.5-kd protein that shares considerable identity with rad6 (66%) as well as with other rad6 homologs, including schizosaccharomyces pombe rhp6+ (70%), drosophila melanogaster dhr6 (83%), and the two human homologs hhr6a an ... | 1995 | 7546294 |
| a carboxylesterase from the parasitic nematode ascaris suum homologous to the intestinal-specific ges-1 esterase of caenorhabditis elegans. | we have identified a carboxylesterase in a. suum that appears to be the homolog of the gut-specific c. elegans ges-1 enzyme. the a. suum esterase was purified and its n-terminal sequence found to be 50% identical to the c. elegans ges-1 protein. we have used isoelectric focusing analysis to demonstrate that, unlike the c. elegans ges-1 esterase, the a. suum enzyme is not restricted to the gut but is expressed in a wide range of tissues. | 1994 | 7553343 |
| the catalytic subunit of camp-dependent protein kinase from ascaris suum. the cloning and structure of a novel subtype of protein kinase a. | a complete cdna clone encoding the catalytic subunit of camp-dependent protein kinase of ascaris suum was constructed from two overlapping partial clones. the encoded sequence of 337 amino acids is 48% identical with the sequence of mouse c alpha subunit. approximately the same low similarity was found with the sequence of the c subunit from another nematode, caenorhabditis elegans. the n-terminal 14 amino acids and the myristoylation site of the mammalian protein are not contained in the enzyme ... | 1995 | 7556139 |
| loa loa: structural diversity of a 15-kda repetitive antigen. | a ladder antigen of loa loa was identified on western blots of all life cycle stages probed with loaisis sera. the smallest subunit has a relative m(r) of about 15 kda and larger subunits represent size increments of 15.0 kda. an 1800-bp genomic clone encoding this antigen was characterized further by restriction mapping. southern blot analysis, and nucleotide sequencing. the antigen is encoded by multiple copies of a gene, linked in tandem repeats of 396 bp, each of which encodes 132 amino acid ... | 1995 | 7556556 |
| the action of serotonin and the nematode neuropeptide ksaymrfamide on the pharyngeal muscle of the parasitic nematode, ascaris suum. | the pharyngeal component of the enteric nervous system of the parasitic nematode, ascaris suum exhibits immunoreactivity for serotonin (5-hydroxytryptamine or 5-ht) and for fmrfamide-like peptides. this paper describes the application of an in vitro pharmacological approach to investigate the functional role of 5-ht and fmrfamide-like peptides. the pharyngeal pumping behaviour of ascaris suum was monitored using a modified pressure transducer system which measures pharyngeal pressure changes and ... | 1995 | 7567105 |