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histomorphological study of the preputial and clitoral glands in balb/c mice with experimental taenia crassiceps infections.male preputial and female clitoral glands of mice undergo development that depends on the level of hormones in the animal. experimental infection with taenia crassiceps cysticerci results in significant physiological modifications in the host. here, we investigated the histomorphological alterations induced by the parasite in these pheromonal glands. preputial and clitoral glands were recovered from mice at 15, 35, 50, and 70 days postinfection (dpi). the glands were examined macroscopically and ...200616629336
dna vaccine against taenia solium cysticercosis expressed as a modified hepatitis b virus core particle containing three epitopes shared by taenia crassiceps and taenia solium.many studies have provided evidence that the hepatitis b core antigen particle is useful as a vaccine carrier for foreign epitopes. epitopes ketc1, ketc12, and gk-1 are three promising candidates for designing a vaccine against taenia solium cysticercosis. in the present study, epitopes ketc1 and ketc12 were inserted into the immunodominant loop of the truncated hbc149, and epitope gk-1 was fused to its c-terminus. the fused protein deltac-3n was expressed and purified successfully. the polymeri ...200516193978
metabolism of steroid hormones by taenia solium and taenia crassiceps cysticerci.previous in vitro experiments showed that both, taenia crassiceps and taenia solium cysticerci have the ability to metabolize exogenous androstenedione to testosterone. here we evaluate on the capacity of both cysticerci to synthesize several sex steroid hormones, using different hormonal precursors. experiments using thin layer chromatography (tlc) showed that both cysticerci were able to produce (3)h-hydroxyprogesterone, (3)h-androstenedione and (3)h-testosterone when (3)h-progesterone was use ...200616644209
a novel synthetic adjuvant effectively enhances the immunogenicity of the influenza vaccine.influenza vaccination is a key intervention to reduce morbidity and mortality provoked by this disease. to date, the challenge of improving its efficacy remains unmet. the immunogenic synthetic peptide gk1 from taenia crassiceps cysticerci was tested herein in its capacity as adjuvant, co-administered with the inactivated anti-influenza vaccine before and after challenge with influenza virus in both young and aged mice. co-administration of gk1 with the influenza vaccine increased levels of anti ...200616202486
intrahepatic dna vaccination: unexpected increased resistance against murine cysticercosis induced by non-specific enhanced immunity.experimental murine cysticercosis caused by taenia crassiceps has proved to be a useful model with which to test the efficacy of new vaccine candidates and delivery systems against pig cysticercosis. a high level of protection against murine cysticercosis was previously observed by intramuscular or intradermal dna immunization with the use of the sequence of the recombinant ketc7 antigen cloned in pcdna3 (ptc-sp7). to determine the effect of ketc7 differential expression in dna vaccination, ketc ...200616884018
protection from murine cysticercosis by immunization with a parasite cysteine protease.central nervous system infection by taenia solium cysts causes neurocysticercosis, a common neurological infection in the third world. we have previously isolated cysteine proteases from taenia crassiceps and t. solium. in this study, we immunized balb/c mice with the purified t. solium cysteine protease and challenged them with taenia crassiceps. immunized mice had a 72% reduction in parasite burden compared to mice that received no immunization. immunized mice developed antigen specific lympho ...200616979365
parasite regulation by host hormones: an old mechanism of host exploitation?recent experimental evidence suggests that parasites can not only evade immune responses actively but also exploit the hormonal microenvironment within the host to favor their establishment, growth and reproduction. the benefit for parasites of hormonal exploitation is so great that they have evolved structures similar to the steroid and protein hormone receptors expressed in upper vertebrates that can bind to the hormonal metabolites synthesized by the host. this strategy is exemplified by two ...200516236553
proinflammatory cytokines in granulomas associated with murine cysticercosis are not the cause of seizures.neurocysticercosis is a parasitic infection of the human central nervous system caused by the cestode taenia solium. the most common clinical manifestations of neurocysticercosis are seizures. taenia crassiceps cysticercosis in mice has been used as an experimental model for t. solium cysticercosis. granulomas surrounding murine cysticerci have striking immunopathological resemblance to human neurocysticercosis; early stage granulomas were able to induce seizures in a rodent model. to assess the ...200616995390
evaluation of the efficacy of albendazole sulphoxide and praziquantel in combination on taenia crassiceps cysts: in vitro studies.praziquantel and albendazole are currently used for chemotherapeutic treatment of neurocysticercosis. albendazole has been found to be more effective than praziquantel; however, it is well known that not all patients will show a complete resolution of cysts. searching for more effective treatments, this study was designed to evaluate the effect of the combination of praziquantel and albendazole sulphoxide in a taenia crassiceps in vitro model as well as the kind of interaction between both drugs ...200616410266
characterization of excretory/secretory endopeptidase and metallo-aminopeptidases from taenia crassiceps metacestodes.cysticercosis is caused by taenia spp. metacestodes, which must survive in the host tissues to complete their life cycle. their survival depends on their control of host immune responses. because many parasites use proteases to modulate host responses, we examined culture media from taenia crassiceps metacestodes for protease activity using peptide substrates. we identified prominent aminopeptidase activity at neutral ph, which was inhibited by chelating agents and partially inhibited by the ami ...200516419737
purification and characterization of a metacestode cysteine proteinase from taenia solium involved in the breakdown of human igg.infection of the central nervous system by taenia solium cysticerci is the cause of human neurocysticercosis, a major neurological infection in the third world and an emerging infectious disease in the united states. we previously isolated a cysteine proteinase from cysticerci of taenia crassiceps and demonstrated that it degrades human igg in vitro. we have now isolated a 48 kda thiol-dependent proteinase from t. solium. the t. solium enzyme also degrades human igg, but does not significantly d ...200516178363
intact glycans from cestode antigens are involved in innate activation of myeloid suppressor cells.during helminthic infections, strong th2 type-biased responses concomitant with impaired cell-proliferative responses to parasitic and unrelated antigens are major immunological hallmarks. parasite glycan structures have been proposed to play a role in modulating these responses. to understand early events related to immune modulation during cestode infection, we have examined the role of intact glycans of antigens from taenia crassiceps in the recruitment of innate cells. soluble antigens from ...200516179033
[cysticercosis contracted in metropolitan france].all cases of cysticercosis diagnosed in france are thought to be imported. our case report concerns a 48-year-old man who has never left europe, in whom we diagnosed subcutaneous cysticercosis.200616493355
systematics of mesocestoides (cestoda: mesocestoididae): evaluation of molecular and morphological variation among isolates.a hypothesis-based framework was used to test if 3 genetic strains of mesocestoides (clades a, b, and c) are distinct evolutionary lineages, thereby supporting their delimitation as species. for comparative purposes, 3 established cestode species, taenia pisiformis, taenia serialis, and taenia crassiceps were assessed using the same methods. sequence data from mitochondrial rdna (12s) and the second internal transcribed spacer of nuclear rdna (its-2) revealed derived (autapomorphic) characters f ...200516539028
carbohydrate components of taenia crassiceps metacestodes display th2-adjuvant and anti-inflammatory properties when co-injected with bystander antigen.common helminth infections promote th2-skewed immune responses in their hosts. we have studied the role of intact carbohydrate structures on taenia crassiceps compounds in the induction of biased type 2 and anti-inflammatory immune responses on peptide-stimulated t cells by using do11.10 transgenic (ova tg) mice. while ova tg mice co-injected with ova peptide (323-339) (ova(323-339)) plus intact taenia soluble antigens (itsa) displayed significantly higher titers of ova-specific igg1 and total i ...200616598470
characterization of the carcinoma-associated tk antigen in helminth parasites.expression of tk antigen, a truncated carbohydrate antigen, was examined in helmith parasites. using the monoclonal antibody lm389, this antigen was detected in extracts from taenia hydatigena, mesocestoides vogae (syn corti), and taenia crassiceps. no reactivity was observed in thysanosoma spp., dipylidium caninum, fasciola hepatica, and nyppostrongylus brasiliensis. on the basis of their electrophoretic mobility, different patterns of tk-bearing glycoproteins were observed among t. hydatigena, ...200717300782
taenia crassiceps organic acids detected in cysticerci.taenia crassiceps cysticerci is used as an experimental model to cysticercosis studies; however there are subcutaneous cases of cysticercosis caused by these cysticerci. it remains unclear in the literature the energetic and fatty acid metabolism in cestodes. its metabolic study may provide knowledge of pathways that may serve as potential anti-helminthic drugs sites of action. in this work we studied the citric acid cycle organic acids and the fatty acid oxidation in cysticerci removed from mic ...200717346707
balb/cj male mice do not feminize after infection with larval taenia crassiceps.balb/cj mice fail to mount an immune response capable of clearing infection with larval taenia crassiceps. additionally, male balb/cj mice display a lag in larval growth of approximately 3 wk as compared to growth in female mice. it has been reported that male balb/ cann mice generate a protective immune response early in infection, and become permissive to larval growth after they feminize (200-fold increase in serum estradiol and 90% decrease in serum testosterone). to determine if a different ...200717436962
cationic supported lipid bilayers for antigen presentation.polystyrene sulfate (pss) particles (301 nm mean diameter) were covered with single cationic dioctadecyldimethylammonium bromide (dda) bilayers and used for antigen adsorption and presentation. the antigen was a mixture of purified 18/14 taenia crassiceps proteins (18/14-tcra). firstly, the dda/pss assembly was characterized at 1mm nacl and 5 x 10(9) pss particles/ml over a range of dda concentrations (0.001-1mm) by means of dynamic light scattering for particle sizing and zeta-potential analysi ...200717452086
ultrastructure of a spermatid transport system in the mature proglottids of experimental taenia crassiceps (wfu strain).in classical textbooks of parasitology, the mature proglottids of taeniids are depicted as structures in which the individual testis are connected to the vas deferens through the vas efferens system, usually depicted as a network of channels. from our morphological analyses of proglottids in the cestode taenia crassiceps, we have been unable to identify this channel network. it is unclear how the spermatids are transported from the testes to the vas deferens, as is unresolved the location of the ...200717522893
subcutaneous taenia crassiceps infection in a patient with non-hodgkin's lymphoma.infections with larvae of taenia crassiceps are rare in humans and have mostly affected patients with acquired immunodeficiency syndrome. we report the first case of a patient with malignancy (non-hodgkin's lymphoma) and infection of the subcutis and muscles of the hand and forearm. surgery and antiparasitic chemotherapy led to a complete cure.200616837716
evaluation of taenia solium and taenia crassiceps cysticercal antigens for immunodiagnosis of neurocysticercosis using elisa on cerebrospinal fluid samples.the efficacy of whole parasite and vesicular fluid antigen extracts from taenia solium and taenia crassiceps cysticerci for immunodiagnosis of neurocysticercosis was evaluated using elisa on cerebrospinal fluid samples. anticysticercal igg antibodies were assayed in cerebrospinal fluid samples from 23 patients with neurocysticercosis and 35 patients with other neurological disorders. the elisa reaction for the whole taenia solium cysticercal extract showed 91.3% sensitivity and 94.3% specificity ...200717568880
modified progesterone receptor expression in the hypothalamus of cysticercotic male mice.progesterone participates in numerous developmental and behavioral processes in the mammalian brain. the intracellular (is this really intracellular? nuclear membrane?) progesterone receptor is expressed as two isoforms: a full-length form (pr-b) and the n-terminally truncated one (pr-a). experimental intraperitoneal infections with taenia crassiceps in mice exhibit the tendency of the parasites to develop more rapidly in females. male mice undergo a feminization process characterized by their o ...200717632066
impact of naturally acquired taenia solium cysticercosis on the hormonal levels of free ranging boars.in chronically infected balbc/ann male mice, taenia crassiceps cysticercosis induces changes in the host's sex steroids hormone that lead to their estrogenization and deandrogenization, with possible repercussions on their susceptibility to infections. here reported are the serum steroid levels in free range cysticercotic male boars. therefore, the possible effects of taenia solium cysticerci over the pig steroid levels were evaluated. herein are described the sex steroids and cortisol levels of ...200717716821
treatment with dehydroepiandrosterone in vivo and in vitro inhibits reproduction, growth and viability of taenia crassiceps metacestodes.the aim of this work was to explore the effect of dehydroepiandrosterone (dhea) on the establishment, growth and reproduction of the metacestode stage of the tapeworm taenia crassiceps, both in vivo and in vitro. administration of dhea prior to infection in mice of both sexes reduced the parasite load by 50% compared with untreated mice. this protective effect was not associated with the immune response, since there was no effect of dhea treatment on mrna levels of il-2, ifn-gamma, il-4 or il-10 ...200818082750
helminth parasites of the wolf canis lupus from latvia.thirty-four wolves were collected between 2003 and 2008 from throughout latvia and examined for helminths. a total of 17 helminth species were recorded: the trematode alaria alata (85.3%); the cestodes diphyllobothrium latum (2.9%), echinococcus granulosus (2.9%), echinococcus multilocularis (5.9%), mesocestoides lineatus (5.9%), taenia crassiceps (8.8%), taenia hydatigena (41.2%), taenia (ovis) krabbei (8.8%), taenia multiceps (47.1%), taenia pisiformis (20.6%), taenia polyacantha (11.8%), taen ...200919138449
antimicrobial peptides (temporin a and iseganan ib-367): effect on the cysticerci of taenia crassiceps.taenia solium infections continue being a health problem in undeveloped countries, and few effective control measures against this parasite are being applied. antimicrobial peptides (amps) belong to the innate immune response and capable of destroying pathogens. we tested the ability of two amps, temporin a (ta) and iseganan ib-367 (ib-367) to damage t. crassiceps cysticerci in vitro. doses of 200 and 400 microg/ml of ta and ib-367 caused cysticerci to shrink, lose motility, the formation of mac ...200919146887
substance p is associated with heart enlargement and apoptosis in murine dilated cardiomyopathy induced by taenia crassiceps infection.dilated cardiomyopathy (degeneration of heart muscle and heart enlargement) is an important cause of heart failure among young adults. dilated cardiomyopathy may be a complication during or after various viral, bacterial, or parasitic diseases. substance p (sp) is a neurotransmitter that is involved in the pathogenesis of various diseases. to determine whether sp is associated with cardiac changes in murine cysticercosis, we compared heart-weight to body-weight ratio, cardiac pathology, cardiomy ...200718163347
induction of interferon-gamma by taenia crassiceps glycans and lewis sugars in naive balb/c spleen and peritoneal exudate cells.helminth parasites are known to alter host immune responses and the responsible molecules are a potential source of biological immunoadjuvants. previously, we have reported strong th-2 type immunomodulatory properties of taenia crassiceps glycans. in this study, we report interferon-gamma (ifn-gamma) stimulatory activity of fractionated taenia glycans and lewis sugars with comparable glycan composition. our data show that taenia glycans and lewis x pentasaccharide are potent stimulators of the t ...200717034862
gonadectomy and progesterone treatment induce protection in murine cysticercosis.the effects of progesterone on castrated mice of both sexes infected with taenia crassiceps cysticerci were studied. gonadectomy and treatment with progesterone before infection decreased parasite loads by 100% compared with intact uninfected mice. mrna levels of ifn-gamma and il-2 (typically associated to th1-like profiles) were markedly decreased in infected gonadectomized (gx) mice, whereas progesterone treatment of infected gx mice did not affected its expression. mrna levels of il-4, and il ...200617096646
efficacy of nitazoxanide, tizoxanide and tizoxanide/albendazole sulphoxide combination against taenia crassiceps cysts.neurocysticercosis is a common parasitic disease in the cns in humans caused by the metacestode taenia solium, with high incidence in developing countries. albendazole is the drug of choice. however, a wide interindividual variability in the response has been reported. in order to evaluate alternative treatment options, the in vitro efficacy of nitazoxanide, its main metabolite tizoxanide as well as the tizoxanide and albendazole sulphoxide combination was tested against taenia crassiceps cysts.200717114175
glucose 6-phosphate dehydrogenase from larval taenia crassiceps (cysticerci): purification and properties.glucose 6-phosphate dehydrogenase (ec 1.1.1.49) was purified to homogeneity from the soluble fraction of larval taenia crassiceps (eucestoda: cyclophyllidea) by a three-step protocol. specific activity of the pure enzyme was 33.8 +/- 2.1 u mg(-1) at 25 degrees c and ph 7.8 with d: -glucose 6-phosphate and nadp+ as substrates. the activity increases to 67.6 +/- 3.9 u mg(-1) at 39 degrees c, a more physiological temperature in the intermediary host. enzyme activity was maximal between ph 6.7 and 7 ...200818297308
acute cysticercosis favours rapid and more severe lesions caused by leishmania major and leishmania mexicana infection, a role for alternatively activated macrophages.parasitic helminths have developed complex mechanisms to modulate host immunity. in the present study we found that previous infection of mice with the cestode taenia crassiceps favours parasitemia and induces larger cutaneous lesions during both leishmania major and leishmania mexicana co-infections. analysis of cytokine responses into draining lymph nodes indicated that co-infection of t. crassiceps-leishmania did not inhibit ifn-gamma production in response to leishmania antigens, but signifi ...200617118349
tamoxifen treatment induces protection in murine cysticercosis.administration of tamoxifen (an antiestrogen) produced an 80% parasite load reduction in female mice, and a weaker effect of 50% in male mice. this protective effect was associated in both sexes, with an increase in the mrna levels of interleukin (il)-2 (a cytokine associated with protection against cysticerci) and il-4 (no effect on infection). tamoxifen treatment modified 17-beta estradiol production in females, whereas serum testosterone was not affected. however, the expression of the 2 type ...200718314701
different effects of chorionic gonadotropin on taenia crassiceps and taenia solium cysticerci cultured in vitro.hormones play a significant role in murine taenia crassiceps cysticercosis, and they may also participate in the susceptibility to taenia solium cysticercosis. in the present study, in vitro effects are reported for human chorionic gonadotropin (hcg) on the larval stages of t. crassiceps (wfu strain) and t. solium. hcg effectively promotes parasite reproduction, i.e., it increases the number of buds on t. crassiceps cysticerci and the percentage of evagination and parasite length in t. solium. t ...200718314702
evagination and infectivity of taenia crassiceps cysticerci in experimental animals.cysticerci of taenia crassiceps reproduce asexually by exogenous budding in the rodent intermediate host, and can experimentally develop to the adult stage within the small intestine of golden hamsters. in the present study, we report the loss of cysticercus infectivity for hamsters after maintaining the strain for 4 yr by consecutive peritoneal passage in mice. larval infectivity was restored after a cysticercus from the wfu strain developed into a gravid tapeworm after being passaged through a ...200818372614
the complex role of pro- and anti-inflammatory cytokines in cysticercosis: immunological lessons from experimental and natural hosts.parasitic helminthes have developed complex mechanisms to evade or modulate hosts responses. studies on cysticercosis are solid, but scarce. the most studied immunological models of cysticercosis are taenia crassiceps infecting mice and t. solium infecting pigs. these parasites, despite being widely exposed to the host, are able to modulate the host immune system. taenia metacestodes, much like other helminthes parasites, have developed complicated strategies in order to infect and successfully ...200818393901
uptake and secretion of host proteins by taenia crassiceps metacestodes.although the presence of intact host proteins in the cyst fluid of cyclophyllidean metacestodes has been well documented, the underlying reason for protein uptake is poorly understood. to investigate this discrepancy, both the cyst fluid (cf) and excreted/secreted (e/s) proteins were collected in vitro from taenia crassiceps metacestodes 16 wk postinfection in balb/cj female mice. the cf and e/s were subsequently immunoblotted using rabbit anti-mouse whole serum antibodies as a probe. the result ...200617152958
nitric oxide contributes to host resistance against experimental taenia crassiceps cysticercosis.the immune mechanisms that underlie resistance and susceptibility to cysticercosis are not completely understood. in this paper, using susceptible balb/c mice and resistant stat6-/-balb/c mice, we have analyzed the role of nitric oxide (no) in determining the outcome of murine cysticercosis caused by the cestode taenia crassiceps. after t. crassiceps infection, wild-type balb/c mice developed a strong th2-like response, produced high levels of igg1, ige, il-5, il-4, and discrete levels of no, an ...200717206501
serodiagnosis of neurocysticercosis in patients with epileptic seizure using elisa and immunoblot assay.sera from 88 patients from santa catarina and são paulo states of brazil, with epileptic seizures who underwent cerebral computed tomography (ct) were analyzed for the detection of antibodies to t. solium cysticercus by elisa and immunoblot (ib) with the following antigens: taenia solium cysticercus total saline (tso), taenia crassiceps cysticercus vesicular fluid (tcra-vf) and t. crassiceps cysticercus glycoproteins (tcra-gp). elisa carried out with tso, tcra-vf and tcra-gp antigens showed 95%, ...200617221132
absence of the sp/sp receptor circuitry in the substance p-precursor knockout mice or sp receptor, neurokinin (nk)1 knockout mice leads to an inhibited cytokine response in granulomas associated with murine taenia crassiceps infection.neurocysticercosis, caused by the cestode taenia solium, is the most common parasitic infection of the human central nervous system that leads to seizures. taenia crassiceps cysticercosis in mice is an experimental model for taenia solium cysticercosis. similar to the human infection, live parasites cause little or no granulomatous inflammation. dying parasites initiate a granulomatous reaction. the neuropeptide, substance p (sp), stimulates t-helper (th) 1 cytokine production. in the current st ...200818576810
synthesis and in vitro cysticidal activity of new benzimidazole derivatives.despite albendazole being the drug of choice in neurocysticercosis treatment, its low solubility limits its bioavailability; therefore, more research is required in order to find new molecules with cestocidal activity and adequate aqueous solubility. a set of 13 benzimidazole derivatives were synthesized and their in vitro activities were evaluated against taenia crassiceps cysts, using albendazole sulfoxide as reference molecule, showing that two of them exhibited good activity. molecular model ...200918582991
two novel ternary albendazole-cyclodextrin-polymer systems: dissolution, bioavailability and efficacy against taenia crassiceps cysts.the effect of two water-soluble polymers: pectin and polyvinylpyrrolidone in combination with beta-cyclodextrin, on the dissolution, bioavailability and cysticidal efficacy of albendazole was evaluated using a commercial suspension as reference product. the dissolution of the albendazole-beta-cyclodextrin-pectin formulation was slow and incomplete (44.7%). no statistical differences in c(max) and auc were found between this formulation and the reference. also its cysticidal efficacy (33%) was si ...201019769931
the key steroidogenic enzyme 3beta-hydroxysteroid dehydrogenase in taenia solium and taenia crassiceps (wfu).larval and adult stages of taenia solium and taenia crassiceps wfu strain were analyzed by histochemical and biochemical methods to determine the existence of steroid pathways. the presence of the key enzyme 3beta-hydroxisteroid-dehydrogenase (3beta-hsd) was examined in frozen sections of cysticerci obtained from mice and segments of tapeworms obtained from the intestine of hamsters. 3beta-hsd activity was detected by nitroblue-tetrazolium products after incubation with dehydroepiandrosterone, a ...200818626663
genetic similarity between cysticerci of taenia solium isolated from human brain and from pigs.mitochondrial (mt) cox1 and ribosomal its1 dna sequences from taenia solium cysticercus isolates from pigs and cysticerci (racemose and cellulose types) from patients with neurocysticercosis were amplified by the polymerase chain reaction (pcr). the amplicons were sequenced in order to determine the genetic relationship between these types of cysticerci. phylogenetic trees were constructed and evolutionary distances were calculated. its1 and mt cox1 cysticerci sequence data were compared with pr ...200818644469
taenia crassiceps: energetic and respiratory metabolism from cysticerci exposed to praziquantel and albendazole in vitro.praziquantel prevents glucose uptake, influencing energy metabolism, while albendazole selectively inhibits the uptake of glucose, leading to glycogen storage depletion in the parasite. the objective of this study was to determine the concentrations of glucose and organic acids related to energy and respiratory metabolisms in in vitro taenia crassiceps cysticerci in the initial, larval and final stages exposed to sub lethal dosages of anti-helminthic drugs. spectrophotometric and chromatographic ...200818694746
taenia crassiceps: chloride currents expressed in xenopus oocytes upon injection of mrna of cysticerci (wfu strain) isolated from mice.to study the properties of ion channels of the tapeworm taenia crassiceps, mrna was isolated from cysticerci and injected into mature oocytes of the frog xenopus laevis and ion currents were recorded four days after injection with the two-electrode voltage clamp technique. oocytes injected with mrna of t. crassiceps expressed outward currents (i(tc)) that activated instantly after onset of the test pulse, followed by a slow inactivation at potentials over +40 mv, with a reversal potential of -23 ...200818706415
5'-p-fluorosulfonyl benzoyl adenosine inhibits an ecto-atp-diphosphohydrolase in the tegument surface of taenia crassiceps cysticerci.the tegumental membrane of taenia crassiceps cysticerci contains an atp-diphosphohydrolase (ec 3.6.1.5) which hydrolyzes purine and pyrimidine nucleoside 5'-di- and 5'-triphosphates at an optimum ph of 8.5. it is mg(2+)-dependent and insensitive to classical atpase and phosphatase inhibitors. in solubilized tegumental membrane the km values varied from 220 to 480 microm and the v(max) from 370 to 748 nmol of pi release/mg/min for nucleoside triphosphates (atp, gtp, ctp, utp, and ttp); for nucleo ...200818765260
taenia crassiceps infection disrupts estrous cycle and reproductive behavior in balb/c female mice.previously, it has been shown that parasitic infections are able to alter the normal mammal physiology, at several extents. thus, we investigated the effects on estrous cycle and sexual behavior induced by intraperitoneal infection with taenia crassiceps in female host mice. along the weeks of infection, parasites were collected from the peritoneal cavity of female mice, showing the maximum parasite load at 16 weeks. no parasites were found outside peritoneal cavity. vaginal estrous cycle was mo ...200919041292
nanobodies, a promising tool for species-specific diagnosis of taenia solium cysticercosis.taenia solium cysticercosis is a major helminth zoonosis in developing countries. pigs are the intermediate hosts mediating transmission of infection. specific assays to diagnose living cysts in pigs are lacking. the monoclonal-based antigen detection elisa is genus-specific and cross-reactions with taenia hydatigena hamper the use of this test to screen pigs. we, therefore, aimed to introduce nanobodies, camelid-derived single-domain antibodies specific for t. solium cysticercosis, to develop u ...200919041315
neuroimmunoendocrine modulation in the host by helminth parasites: a novel form of host-parasite coevolution?helminth parasites have evolved diverse molecular mechanisms that facilitate their establishment, growth and reproduction inside an immunologically hostile environment. thus, the physiological interactions during the course of the immune response to helminths are complex. infection induces antigen-specific recognition by the immune system, which is consequently charged with the responsibility of marshalling the appropriate effector responses necessary to destroy the parasite, or at the very leas ...200919212127
neonatal exposure to estradiol induces resistance to helminth infection and changes in the expression of sex steroid hormone receptors in the brain and spleen in adult mice of both sexes.a single injection of 17beta-estradiol administered to 4-day-old male and female mice increased the cellular immune response, and induced resistance to taenia crassiceps cysticercosis as well as changes in the expression pattern of progesterone (pr) and estrogen receptor (er) isoforms in the brain and splenocytes. regardless of gender, when treated mice reached adulthood, they were highly resistant to infection. female mice presented early vaginal opening and altered estrous cycles. in male and ...200919258031
substance p signaling contributes to granuloma formation in taenia crassiceps infection, a murine model of cysticercosis.cysticercosis is an infection with larval cysts of the cestode taenia solium. through pathways that are incompletely understood, dying parasites initiate a granulomatous reaction that, in the brain, causes seizures. substance p (sp), a neuropeptide involved in pain-transmission, contributes to inflammation and previously was detected in granulomas associated with dead t. crassiceps cysts. to determine if sp contributes to granuloma formation, we measured granuloma-size and levels of il-1beta, tn ...201020150970
taenia crassiceps: fatty acids oxidation and alternative energy source in in vitro cysticerci exposed to anthelminthic drugs.cysticerci metabolic studies demonstrate alternative pathways responsible for its survival, such as energy sources, fatty acids oxidation and excretion of beta-hydroxybutyrate, which indicates the capability of energy production from proteins. the aim of this study was to detect alternative metabolic pathways for energy production and its end products in taenia crassiceps cysticerci in vitro exposed to praziquantel and albendazole, in sub-lethal doses. spectrophotometer and chromatographic analy ...200919348802
toxocariasis/cysticercosis seroprevalence in a long-term rural settlement, são paulo, brazil.seroprevalence of toxocara and taenia solium and risk factors for infection with these parasites were explored in a long-term rural settlement in são paulo state, brazil. an elisa for the detection of anti-toxocara igg and ige and anti-t. solium cysticerci was standardized using toxocara excretory-secretory antigens (tes) obtained from the cultured second-stage larvae of t. canis and by vesicular fluid antigen from taenia crassiceps cysticerci (vf). for cysticercosis, the reactive elisa samples ...200919366477
transglycosylation-based fluorescent labeling of 6-gala series glycolipids by endogalactosylceramidase.although 6-gala series glycosphingolipids possessing r-gal (alpha/beta) 1-6gal beta 1-1'cer have been found in some mollusks, pathogenic parasites, and fungi, their physiological functions and metabolic pathway are not fully understood. we described a novel method of detecting 6-gala series glyco- sphingolipids utilizing the specificity of endogalactosylceramidase (egalc), which is capable of hydrolyzing 6-gala series glycosphingolipids to produce intact oligosaccharides and ceramides. egalc cat ...200919389917
the carriage of larval echinococcus multilocularis and other cestodes by the musk rat (ondatra zibethicus) along the ourthe river and its tributaries (belgium).in belgium, the carriage of echinococcus multilocularis by the red fox (vulpes vulpes) can be very high in some areas. this study was designed to evaluate the carriage of the larval form of e. multilocularis and other cestodes in a musk rat (ondatra zibethicus) population trapped along the ourthe river (southeastern belgium). six hundred fifty-seven musk rats were necropsied, and the larval cestodes of the abdominal and pleural cavities were identified. for e. multilocularis, the fertility of th ...200919395737
characterization of one typical 2-cys peroxiredoxin gene of taenia solium and taenia crassiceps.the taenia genus is capable of living for long periods within its hosts. reports have shown that this successful establishment is related to its efficient defense mechanisms against host immune response and its high tolerance to oxidative stress. in this work, we describe the genomic sequences of one taenia solium and taenia crassiceps typical 2-cys peroxiredoxins (ts2-cysprx, tc2-cysprx) genes, which are 94% identical in primary sequence with the typical 2-cys prxs catalytic motifs. both genes ...200919424718
differential response of antigen presenting cells from susceptible and resistant strains of mice to taenia crassiceps infection.antigen presenting cells (apcs) are critically involved in the interaction between pathogens and the host immune system. here, we examined two different populations of apcs in mice that are susceptible (balb/c) or resistant (c57bl/6) to taenia crassiceps cysticercosis. bone marrow-derived dendritic cells (bmdcs) from both strains of mice were exposed to t. crassiceps excreted/secreted antigens (tces) and, at the same time, to the toll-like receptor (tlr) ligand lps. bmdcs from balb/c mice underw ...200919465163
differential in vitro effects of insulin on taenia crassiceps and taenia solium cysticerci.hormones play a significant role in murine cysticercosis (taenia crassiceps), and increase the frequency of porcine cysticercosis caused by taenia solium. in the present study, we report the in vitro effect of insulin on the larval stages of t. crassiceps (orf strain) and t. solium. in vitro exposure of t. crassiceps cysticerci to insulin was found to stimulate this parasite's reproduction twofold with respect to control values, while the same treatment had no effect on t. solium cysticerci. mor ...200919549345
towards identification of the mechanisms of action of parasite-derived peptide gk1 on the immunogenicity of an influenza vaccine.previous studies have shown that the synthetic peptide gk1, derived from taenia crassiceps cysticerci, enhances the immunogenicity of the commercial inactivated influenza vaccine fluzone in both young and aged mice. in particular, antibody responses were much improved. since gk1 is a peptide and is rapidly cleared from the body, it offers the possibility to improve vaccine performance without undesirable effects. this study was therefore designed to understand the mechanisms of action involved i ...200919605594
taenia crassiceps: infections of male mice lead to severe disruption of seminiferous tubule cells and increased apoptosis.this research was carried out to study the effects of infection with taenia crassiceps cysticerci on the seminiferous epithelium histoarchitecture in the testes of male mice. our results showed a severe disruption of the histoarchitecture of the testis epithelium in infected mice. in these animals, a significant infiltration of macrophages within seminiferous tubules was observed (p < 0.001). generalized apoptosis of germ cells within the seminiferous tubules was observed, as assessed by tunel a ...201120647013
chorionic gonadotropin hormone receptors on taenia solium and taenia crassiceps cysticerci in culture.hormones play a significant role in murine taenia crassiceps cysticercosis, and they may also participate in the susceptibility to taenia solium cysticercosis. in the present study, in vitro effects are reported for human chorionic gonadotropin (hcg) on the larval stages of t. crassiceps (wfu strain) and t. solium. our results reveal the presence of receptors for hcg in different developmental phases of both cultured parasites. on day 30, both taeniid species had the highest percentage of recept ...200919656000
the neuroimmunoendocrine network in the complex host-parasite relationship during murine cysticercosis.we review here the role that sex steroids play in experimental intraperitoneal taenia crassiceps cysticercosis of male and female balbc/ann mice. briefly, estrogens favour and androgens hinder the reproduction of cysticerci by at least two main mechanisms: 1) through estradiol tilting the th2/th1 immune system balance towards parasite-permissive th2 response,which is il-6 dependent mediating p450-aromatase over expression, shunting testosterone towards estradiol and thus creating a positive feed ...200818393903
preferential growth of taenia crassiceps cysticerci in female mice holds across several laboratory mice strains and parasite lines.a retrospective study of our 14-yr records on experimental taenia crassiceps (orf(fast) line) cysticercosis (n = 1,198) shows that in 16 of 17 different mice strains, female mice are more frequently infected and carry larger individual parasite loads than males. however, sexual differences in parasite loads significantly varies between strains in relation to their different genetic backgrounds (balb > c57bl = others > c3h). the coefficient of variation in all female mice is significantly smaller ...200818564762
novel immunoadjuvants based on cationic lipid: preparation, characterization and activity in vivo.the interactions between three different protein antigens and dioctadecyldimethylammonium bromide (dodab) dispersed in aqueous solutions from probe sonication or adsorbed as one bilayer onto particles was comparatively investigated. the three model proteins were bovine serum albumin (bsa), purified 18 kda/14 kda antigens from taenia crassiceps (18/14-tcra) and a recombinant, heat-shock protein hsp-18 kda from mycobacterium leprae. protein-dodab complexes in water solution were characterized by d ...200919664738
taenia crassiceps cysticercosis: variations in its parasite growth permissiveness that encounter with local immune features in balb/c substrains.this study describes the first days of taenia crassiceps infection in balb/c substrains, balb/cann and balb/cj, using two stocks of the same strains which were kept in different animal facilities, conventional and pathogen-free conditions, respectively. this study shows that parasite growth restriction shown by conventional balb/cj mice changed to parasite growth permissiveness when pathogen-free balb/cj mice were used. in addition, the higher number of macrophages, nk cells and intraperitoneal ...200919735657
strategies of statistical image analysis of 2d immunoblots: the case of igg response in experimental taenia crassiceps cysticercosis.a procedure is described to measure the diversity and enrich the meaning and usefulness of the information contained in 2d immunoblot images of the reaction between a complex mixture of parasite antigens and the complex set of antibodies usually present in the sera of infected individual hosts. the procedure and results are illustrated by the experimental infection of 30 mice (three strains, both sexes, 5 mice in each strain x sex combination) with taenia crassiceps cysticerci, thirty days after ...200919800348
taenia crassiceps: in vivo and in vitro models.taenia crassiceps is a cestode parasite of wild and domestic animals that rarely affects humans; it has been widely used as an experimental model. the asexual proliferation by budding is a useful attribute of t. crassiceps cysticerci, which allows the various strains to be maintained indefinitely in the peritoneal cavity of inbred mice. over the last 50 years, experimental results using larval and adult stages of t. crassiceps have yielded much information on the morphology, infectivity, prolife ...201019835660
taenia crassiceps cysticerci: characterization of the 14-kda glycoprotein with homologies to antigens from taenia solium cysticerci.glycoproteins from the total vesicular fluid of taenia crassiceps (vf-tc) were prepared using three different purification methods, consisting of cona-lectin affinity chromatography (cona-tc), preparative electrophoresis (sds-page) (14 gp-tc), and monoclonal antibody immunoaffinity chromatography (18/14-tc). the complex composition represented by the vf-tc and cona-tc antigens revealed peptides ranging from 101- to 14-kda and from 92- to 12-kda, respectively. immunoblotting using lectins confirm ...201019896939
taenia crassiceps subcutaneous cysticercosis in an adult dog. 200919966334
early removal of alternatively activated macrophages leads to taenia crassiceps cysticercosis clearance in vivo.to determine the role of alternatively activated macrophages in modulating the outcome of experimental cysticercosis caused by taenia crassiceps, we investigated the effect of removal of alternatively activated macrophage by injecting clodronate-loaded liposomes into susceptible balb/c mice. following t. crassiceps infection, mice receiving pbs-loaded liposomes developed a dominant th2-type response associated with the presence of alternatively activated macrophages together with antigen-specifi ...201020045000
taenia crassiceps infection attenuates multiple low-dose streptozotocin-induced diabetes.taenia crassiceps, like other helminths, can exert regulatory effects on the immune system of its host. this study investigates the effect of chronic t. crassiceps infection on the outcome of multiple low dose streptozotocin-induced diabetes (mlds). healthy or previously t. crassiceps-infected mice received mlds and type 1 diabetes (t1d) symptoms were evaluated for 6 weeks following the induction of mlds. t. crassiceps-infected mice displayed lower blood glucose levels throughout the study. a si ...201020069130
altered expression of cytokines and sex steroid receptors in the reproductive tract of cysticercotic male mice.infection with taenia crassiceps cysticerci in male mice produces an increase in serum oestradiol levels, whereas serum testosterone is abolished. concomitantly, complete atrophy of the reproductive tract of infected male mice is observed. the present study was undertaken to determine the expression pattern of cytokines involved in steroidogenesis and sex steroid receptors in the reproductive tissues of normal and infected male mice, and relating this expression pattern to whole parasite counts, ...201020070823
a new map kinase protein involved in estradiol-stimulated reproduction of the helminth parasite taenia crassiceps.map kinases (mapk) are involved in the regulation of cellular processes such as reproduction and growth. in parasites, the role of mapk has been scarcely studied. here, we describe the participation of an erk-like protein in estrogen-dependent reproduction of the helminth parasite taenia crassiceps. our results show that 17beta-estradiol induces a concentration-dependent increase in the bud number of in vitro cultured cysticerci. if parasites are also incubated in presence of an erk-inhibitor, t ...201020145710
extraintestinal helminths of the common vole (microtus arvalis) and the water vole (arvicola terrestris) in western austria (vorarlberg).between september and december 2004, a total of 411 voles (318 common voles and 93 water voles) were caught in the austrian province of vorarlberg (lustenau, hohenems, and dornbirn) and were examined by macroscopy, microscopy, and molecular biological analysis to determine the presence and extent of medically important extraintestinal helminths. the following extraintestinal helminth species were detected: taenia taeniaeformis (liver), calodium hepaticum (liver), and echinococcus multilocularis ...201020148339
budding of taenia crassiceps cysticerci in vitro is promoted by crowding in addition to hormonal, stress, and energy-related signals.taenia crassiceps cysticerci (cysts) reproduce by budding. the cysts' production of buds was measured in vitro to explore parasite and environmental-related factors involved in the extreme individual variation in parasite loads of inbred mice. cysts were placed in in vitro culture for 10 days at initial parasite densities of 1, 5, 10 cysts/well in 1 ml of rpmi medium 1640 without serum. results showed that there is considerable intrinsic initial variation among inoculated cysts in their producti ...201020168999
impaired pro-inflammatory cytokine production and increased th2-biasing ability of dendritic cells exposed to taenia excreted/secreted antigens: a critical role for carbohydrates but not for stat6 signaling.in cysticercosis, a parasitic disease caused by cestodes, the details of early interactions between parasite antigens and innate cells from the host are not well understood. in this study, the role of cestode-conditioned dendritic cells (dcs) in priming th1 versus th2 responses to bystander antigen was examined by using cd11c(+) dcs as antigen-presenting cells and naive cd4(+) do11.10 lymphocytes specific to ovalbumin (ova) as responding cells. no conventional maturation was induced in dcs expos ...201020361966
expression of the inhibitory cd200 receptor is associated with alternative macrophage activation.classical macrophage activation is inhibited by the cd200 receptor (cd200r). here, we show that cd200r expression was specifically induced on human in vitro polarized macrophages of the alternatively activated m2a subtype, generated by incubation with il-4 or il-13. in mice, peritoneal m2 macrophages, elicited during infection with the parasites taenia crassiceps or trypanosoma brucei brucei, expressed increased cd200r levels compared to those derived from uninfected mice. however, in vitrostimu ...201020375636
parasiticidal effect of 16alpha-bromoepiandrosterone (epibr) in amoebiasis and cysticercosis.the effect of the dehydroepiandrosterone analog 16alpha-bromoepiandrosterone (epibr) was tested on the tapeworm taenia crassiceps and the protist entamoeba histolytica, both in vivo and in vitro. administration of epibr prior to infection with cysticerci in mice reduced the parasite load by 50% compared with controls. epibr treatment induced 20% reduction on the development of amoebic liver abscesses in hamsters. in vitro treatment of t. crassiceps and e. histolytica cultures with epibr, reduced ...201020403456
in vitro killing action of auranofin on taenia crassiceps metacestode (cysticerci) and inactivation of thioredoxin-glutathione reductase (tgr).control of cellular redox homeostasis is a central issue for all living organisms. glutathione and thioredoxin enzymatic redox systems are the usual mean used to achieve such a control. however, parasitic platyhelminths studied to date possess a nicotinamide adenine dinucleotide phosphate-dependent thioredoxin-glutathione reductase (tgr) as the sole redox control system. thus, tgr is considered as a potential therapeutic target of parasitic platyhelminths, and based on this assumption, the gold ...201020431894
characterization of s3pvac anti-cysticercosis vaccine components: implications for the development of an anti-cestodiasis vaccine.cysticercosis and hydatidosis seriously affect human health and are responsible for considerable economic loss in animal husbandry in non-developed and developed countries. s3pvac and eg95 are the only field trial-tested vaccine candidates against cysticercosis and hydatidosis, respectively. s3pvac is composed of three peptides (ketc1, gk1 and ketc12), originally identified in a taenia crassiceps cdna library. s3pvac synthetically and recombinantly expressed is effective against experimentally a ...201020585656
taenia crassiceps cysticercosis in a ring-tailed lemur (lemur catta).subcutaneous and intraperitoneal cysticercosis due to taenia crassiceps was diagnosed in a 5-yr-old male ring-tailed lemur (lemur catta) in the madrid zoo-aquarium (madrid, spain). under laparoscopic examination, several septated fibrous cystic structures and numerous masses of small transparent vesicles (ca. 3 mm in diameter) were observed subcutaneously and inside the peritoneal cavity. most of the structures were extirpated but, after 2 days of postsurgical intensive care, the animal died. th ...201020597227
in vitro evaluation of the effects of cysticidal drugs in the taenia crassiceps cysticerci orf strain using the fluorescent celltracker cmfda.using a murine model of cysticercosis caused by the taenia crassiceps orf strain, we developed a fluorescent quantitative evaluation of the action of two well known anti-helminthic drugs: albendazole sulfoxide and praziquantel. the fluorescence emitted by a biotransformed celltracker probe known as celltracker green cmfda in the vesicular fluids of cysticerci was estimated, and the results were compared with macroscopic observations of the parasites. the pharmacological ec(50) value of each drug ...201120599436
decrease of peritoneal inflammatory cd4(+), cd8(+), cd19(+) lymphocytes and apoptosis of eosinophils in a murine taenia crassiceps infection.after an intraperitoneal infection of mice with taenia crassiceps metacestodes, peritoneal inflammatory cells labeled with fluoresceinated moab anti-mouse were analyzed by flow cytometry. apoptosis was studied by annexin a/pi, tunel assays, dna laddering, caspase-3 activity, and electron microscopy. an important continuous decrease of cd4+, cd8+ and cd19+ lymphocytes, and an increase of eosinophils and macrophages throughout the observation time were found. apoptosis of eosinophils was quantifie ...201020706737
mitochondrial thioredoxin-glutathione reductase from larval taenia crassiceps (cysticerci).mitochondrial thioredoxin-glutathione reductase was purified from larval taenia crassiceps (cysticerci). the preparation showed nadph-dependent reductase activity with either thioredoxin or gssg, and was able to perform thiol/disulfide exchange reactions. at 25 degrees c specific activities were 437 +/- 27 mu mg(-1) and 840 +/- 49 mu mg(-1) with thioredoxin and gssg, respectively. apparent k(m) values were 0.87 +/- 0.04 mum, 41 +/- 6 mum and 19 +/- 10 mum for thioredoxin, gssg and nadph, respect ...201020798751
intestinal parasites of the arctic fox in relation to the abundance and distribution of intermediate hosts.the intestinal parasite community of arctic foxes (vulpes lagopus) on the svalbard archipelago in the high arctic was investigated in relation to the abundance and distribution of intermediate hosts. five species of cestodes (echinococcus multilocularis, taenia crassiceps, taenia polyacantha, taenia krabbei and diphyllobothrium sp.), ascaridoid nematodes and one unidentified acanthocephalan species were found. the cestodes e. multilocularis, t. crassiceps and t. polyacantha all showed a decreasi ...201019723357
alternatively activated macrophages engage in homotypic and heterotypic interactions through il-4 and polyamine-induced e-cadherin/catenin complexes.alternatively activated macrophages (aams), triggered by interleukin-4 (il-4) and il-13, play a modulating role during th2 cytokine-driven pathologies, but their molecular armament remains poorly characterized. here, we established e-cadherin (cdh1) as a selective marker for il-4/il-13-exposed mouse and human macrophages, which is stat6-dependently induced during polarized th2 responses associated with taenia crassiceps helminth infections or allergic airway inflammation. the il-4-dependent, arg ...200919726720
cpg stimulates protective immunity in balb/cj mice infected with larval taenia crassiceps.balb/cj mice infected in the peritoneal cavity with larval taenia crassiceps fail to mount a protective immune response. in mice, inflammatory immune responses are believed to control larval reproduction, whereas antibody-mediated responses are believed to be permissive. in the present study, mice were treated with cpg-oligodeoxynucleotides (cpg) to determine whether stimulation of the innate inflammatory response would confer increased resistance to larval growth. female mice treated with cpg d ...201020950099
fact or hypothesis: taenia crassiceps as a model for taenia solium, and the s3pvac vaccine.research undertaken over the past 40 years has established many of the general principals concerning immunity to taeniid cestodes. although much is well understood about the host-protective mechanisms against taeniids and this knowledge has been exploited in studies on vaccine development, many aspects require further investigation or confirmation. some phenomena have come to be regarded as being well established, while careful analysis of the published data would suggest that they may be better ...201021039610
development of pcr/dot blot assay for specific detection and differentiation of taeniid cestode eggs in canids.we report the development of a colourimetric pcr/dot blot assay targeting the mitochondrial gene nadh dehydrogenase subunit 1 (nad1) for differential diagnosis of taeniid eggs. partial sequences of the cestode nad1 gene were aligned and new primers were designed based on conserved regions. species-specific oligonucleotide probes (s-sonp) for canine taeniid cestodes were then designed manually based on the variable region between the conserved primers. specifically, s-sonp were designed for the t ...201021112414
taenia crassiceps as a model for taenia solium and the s3pvac vaccine. 201121155844
taenia crassiceps infection abrogates experimental autoimmune encephalomyelitis.helminth infections induce strong immunoregulation that can modulate subsequent pathogenic challenges. taenia crassiceps causes a chronic infection that induces a th2-biased response and modulates the host cellular immune response, including reduced lymphoproliferation in response to mitogens, impaired antigen presentation and the recruitment of suppressive alternatively activated macrophages (aamф). in this study, we aimed to evaluate the ability of t. crassiceps to reduce the severity of exper ...201021185554
a real-time multiplex-nested pcr system for coprological diagnosis of echinococcus multilocularis and host species.a hybridization probe-based real-time multiplex-nested pcr system was developed for the simultaneous detection of echinococcus multilocularis and host species directly from faecal samples. species identification was determined by melting curve analysis. specificity was assessed by using dna extracted from various cestodes (e. multilocularis, echinococcus granulosus (g1), echinococcus ortleppi, echinococcus canadensis (g6, g7), taenia crassiceps, taenia hydatigena, taenia mustelae, taenia pisifor ...201121327991
taenia crassiceps upper limb fasciitis in a patient with untreated acquired immunodeficiency syndrome and chronic hepatitis c infection--the role of surgical debridement.we report a rare case of human taenia crassiceps infection in a 47-year-old female patient with untreated acquired immunodeficiency syndrome and chronic hepatitis c infection. little experience exists regarding the appropriate treatment of this infection. usually, a combination of anthelmintic drugs is applied. whether surgical measures are indicated have not been clarified. in our patient, initial surgery showed an abscess and fluid collection with numerous transparent cysts localised in the su ...201121385665
taenia crassiceps: a secretion-substance of low molecular weight leads to disruption and apoptosis of seminiferous epithelium cells in male mice.the present research was performed to isolate and study the effects of a low molecular weight (<1300da) parasite-associated substance, obtained from peritoneal fluids of female mice infected with taenia crassiceps cysticerci, on seminiferous epithelium cells of male mice testis. the results showed an intense disruption of sertoli cells and germ cells within the seminiferous tubules of experimental mice, along with the destruction of their gap junction (gj). significant generalized apoptosis of g ...201121440545
heterologous prime-boost oral immunization with gk-1 peptide from taenia crassiceps cysticerci induces protective immunity.oral immunization is a goal in vaccine development, particularly for pathogens that enter the host through the mucosal system. this study was designed to explore the immunogenic properties of the taenia crassiceps protective peptide gk1 orally administered. mice were orally immunized with the synthetic gk1 peptide in its linear form with or without the brucella lumazine synthase protein (bls) adjuvant or as a chimera recombinantly bound to bls (bls-gk1). each 15 days later, mice were boosted twi ...201121593234
a helminth cestode parasite express an estrogen-binding protein resembling classic nuclear estrogen receptor.the role of an estrogen-binding protein similar to a known mammalian estrogen receptor (er) is described in the estradiol-dependent reproduction of the helminth parasite taenia crassiceps. previous results have shown that 17-β-estradiol induces a concentration-dependent increase in bud number of in vitro cultured cysticerci. this effect is inhibited when parasites are also incubated in the presence of an er binding-inhibitor (tamoxifen). rt-pcr assays using specific oligonucleotides of the most ...201121621550
seroepidemiological study of human cysticercosis with blood samples collected on filter paper, in lages, state of santa catarina, brazil, 2004-2005.human serofrequency of antibodies against taenia solium antigens was determined and risk factors for cysticercosis transmission were identified.201121739072
Identification of Loci Controlling Restriction of Parasite Growth in Experimental Taenia crassiceps Cysticercosis.Human neurocysticercosis (NC) caused by Taenia solium is a parasitic disease of the central nervous system that is endemic in many developing countries. In this study, a genetic approach using the murine intraperitoneal cysticercosis caused by the related cestode Taenia crassiceps was employed to identify host factors that regulate the establishment and proliferation of the parasite. A/J mice are permissive to T. crassiceps infection while C57BL/6J mice (B6) are comparatively restrictive, with a ...201122206032
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