Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| taenia crassiceps cysticercosis: a role for prostaglandin e2 in susceptibility. | several biological factors are involved in susceptibility and resistance to murine cysticercosis. a substantial body of evidence implies prostaglandins as potent regulators of immune responses during parasitic diseases. here we evaluated the role played by prostaglandin e2 in cysticercosis. mice were treated in vivo with prostaglandin e2 or with indomethacin (a prostaglandin e2 synthesis inhibitor) before infection. parasite growth was enhanced by prostaglandin treatment, which provoked poor con ... | 1999 | 10599927 |
| specific taenia crassiceps and taenia solium antigenic peptides for neurocysticercosis immunodiagnosis using serum samples. | neurocysticercosis (nc), i.e., the presence of the larval form of taenia solium in tissues, is the most frequent and severe infection involving the central nervous system. paired serum and cerebrospinal fluid (csf) samples from patients with nc, csf and serum samples from a control group, and serum samples from patients with other parasitoses were studied by enzyme-linked immunosorbent assay (elisa) and by immunoblotting with taenia crassiceps vesicular fluid antigen (tcra) and taenia solium tot ... | 2000 | 10618078 |
| fatal cysticercosis by taenia crassiceps (cyclophyllidea: taeniidae) in a presumed immunocompromised canine host. | cysticercosis in a canine host (canis familiaris) attributable to the taeniid cestode taenia crassiceps is reported for the first time in north america. numerous parent and daughter cysticerci occurred in a massive intrapleural and intraperitoneal infection in an apparently immunocompromised host. the largest cysticerci were ovoid to elongate, 5-9 mm in maximum length, and armed with 32-34 rostellar hooks in 2 rows; small hooks measured 114-143 microm long (x = 124+/-8.2 microm), and large hooks ... | 1999 | 10647054 |
| performance of the elisa test for swine cysticercosis using antigens of taenia solium and taenia crassiceps cysticerci. | studies were conducted to evaluate antigens of taenia solium (tso) and taenia crassiceps (tcra) cysticerci in the elisa test for the diagnosis of swine cysticercosis. the samples analyzed were cysticercosis positive and negative control sera and heterologous sera. four antigens were assayed: vesicular fluid (vf) and crude (t) tcra and scolex (s) and crude (t) tso. all antigens showed good performance, but vf-tcra was the best followed by t-tcra. sensitivity rates of elisa were respectively, in 2 ... | 2000 | 10681029 |
| taenia crassiceps cysticercosis: protective effect and immune response elicited by dna immunization. | the nucleotide sequence of a protective recombinant antigen of taenia crassiceps cysticerci present in all stages of taenia solium (ketc7), cloned into pcdna3 plasmid with the signal peptide sequence of the beta-glycan receptor (ptc-sp7), has been shown to be effective in protecting mice against experimental infection of t. crassiceps. to explore further the possibilities of this form of immunization and the immune response induced, mice were injected intramuscularly (i.m.) or intradermally (i.d ... | 2000 | 10701566 |
| cysticercosis: towards the design of a diagnostic kit based on synthetic peptides. | cysticercosis caused by taenia solium is a very common disease in developing countries that seriously affects human health. diagnosis can only be confirmed with the aid of computerized tomography or nuclear magnetic resonance (nmr) creating obvious difficulties for epidemiological studies. reliable immunoassays employing cerebrospinal fluid (csf) have been developed, based on the use of cysticercal antigens. however, the reliance on parasite material is restrictive. herein, we report the advance ... | 2000 | 10709780 |
| two taenia species found in japan, with new distribution record of taenia polyacantha leuckart, 1856 (cestoda: taeniidae). | in an epidemiological survey for echinococcus multilocularis in rodents and insectivores from the northernmost part of the central mainland of japan (honshu), two taeniid species, taenia crassiceps and taenia polyacantha, were found in microtus montebelli and apodemus argenteus, respectively. the latter is the first record of distribution in japan, and the former is the second after its first recovery from the central part of japan. although we have found neither larval nor strobilar stage of e. ... | 2000 | 10725694 |
| immune destruction of larval taenia crassiceps in mice. | immune destruction of larval taenia crassiceps was examined by first injecting balb/cj mice subcutaneously with larval buds and 30 to 60 days later challenging the mice with larvae injected into the peritoneal cavity. the larvae injected intraperitoneally (i.p.) secondarily are killed by host cells that completely encase the larvae in a thick sheath. the peritoneal exudate cells and the cytokines they produced were characterized by flow cytometry, enzyme-linked immunosorbent assays (elisas), and ... | 2000 | 10768922 |
| neurocysticercosis: detection of igg, iga and ige antibodies in cerebrospinal fluid, serum and saliva samples by elisa with taenia solium and taenia crassiceps antigens. | we assayed samples of cerebrospinal fluid (csf), serum and saliva from patients with neurocysticercoses, control group and individuals with other parasitoses, by elisa with taenia crassiceps vesicular fluid antigen (tcra) and taenia solium total antigen (tso) for the detection of antibodies. the sensitivity for igg-tcra was 100% for csf and serum, and 32.0% for saliva; and for igg-tso 100% for csf, 80.0% for serum and 24.% for saliva. specificity was 100% for csf and 80.0% for serum with both an ... | 2000 | 10770861 |
| survival of destrobilated adults of taenia crassiceps in t-cell-depleted mongolian gerbils. | in mongolian gerbils (meriones unguiculatus), prednisolone treatment induces the survival of strobilated taenia crassiceps to sexual maturity followed by fecal release of gravid proglottides. the mechanism underlying the effects of prednisolone has not been elucidated in this taeniid/rodent model. using a novel murine monoclonal antibody specific to a cell-surface determinant of gerbil t-cells (husm-m.g.15 of igg2b isotype) for in vivo depletion of the cells, we examined the t-cell dependence of ... | 2000 | 10780736 |
| cross-reactivity of anti-taenia crassiceps cysticerci immune antibodies with taenia solium antigens. | a comparative study of antibody production was carried out using balb/c mice immunized with 20 or 50microg vesicular fluid (vf)-tcra (taenia crassiceps) antigens, and gel of <30kd or eluate from <30kd peptides. good igm, iga and igg levels were detected by elisa-tcra and the antibodies presented reactivity with the <20kd peptides when tested by immunoblotting-tcra. the antibodies from animals immunized with 20 and 50microg presented high anti-tso cross-reactivity in elisa (igg>>igm and iga). all ... | 2000 | 10799846 |
| elisa test for the diagnosis of cysticercosis in pigs using antigens of taenia solium and taenia crassiceps cysticerci. | in the present study elisa was standardized for the diagnosis of swine cysticercosis based on necropsy parameters and confirmed positive and negative control sera. serum samples from pigs with other infections were also assayed to determine possible cross-reactions. four antigens were assayed: from taenia crassiceps vesicular fluid (vf-tcra) and crude larvae extract (t-tcra), and from taenia solium extracts of scolex (s-ts) and of larvae (t-ts). a checkerboard evaluation of antigen, serum and co ... | 2000 | 10810321 |
| parasite-secreted products regulate the host response to larval taenia crassiceps. | parasite-induced immunosuppression is believed to play a significant role in the pathology of cysticercosis, a disease caused by the larval stage of cestode parasites. the biochemical basis for immunoregulation by taenia crassiceps in experimental cysicercosis is unknown. in order to determine whether or not excretory/secretory (e/s) products from the parasite have the ability to regulate host immune function, the activity of these products was examined. excretory/secretory products from larvae ... | 2000 | 10849309 |
| apoptosis in human jurkat t cells after culture with live taenia crassiceps cysticerci in vitro. | this study examines the effects of taenia crassiceps cysticerci on the viability of a human t lymphocyte cell line (jurkat). both budding and non-budding t. crassiceps metacestodes were cultured over 24 and 48 h in the presence of jurkat cells. cell viability decreased with increasing numbers of cysticerci, particularly budding cysticerci. single cell gel electrophoresis (comet) analysis, which grades dna damage, showed a significant increase in apoptosis at 24 and 48 h. the morphology of treate ... | 2000 | 10874728 |
| production of monoclonal antibodies anti-taenia crassiceps cysticerci with cross-reactivity with taenia solium antigens. | we describe the production of the potential monoclonal antibodies (moabs) using balb/c mice immunized with vesicular fluid (vf)-tcra (t. crassiceps) antigen. immune sera presented anti-vf-tcra (<20kd) igg and igm antibodies with cross-reactivity with t. solium (tso) antigen (8-12, 14, and 18 kd). after cell fusion, we selected 33 anti-tcra and anti-tso reactive igm-clones and 53 anti-tcra specific igg-clones, 5 of them also recognizing tso antigens. two clones identified the 8-14 and 18kd peptid ... | 2000 | 10887380 |
| changes in hepatic lipids of mice infected with cysticerci of taenia crassiceps. | nuclear magnetic resonance (nmr) spectroscopy was employed to investigate the effect of infection with taenia crassiceps cysticerci on the lipid profile of mouse liver. chloroform/methanol extracts of livers from infected mice showed lower concentrations of phosphatidylethanolamine, phosphatidylcholine, phosphatidylinositol, total glycerophospholipid, triacylglycerol, total fatty acid (fa) and all measured fa components than those from controls. furthermore, the ratios obtained on dividing conce ... | 2000 | 10953220 |
| immunofluorescent localization of intermediate filaments (ifs) in helminths using anti-mammalian ifs monoclonal antibody. | intermediate filaments (ifs) make up the cytoskeleton of most eukaryotic cells. in vertebrates, a number of if proteins have been identified, showing distributions unique to tissue or cell type. information on helminth ifs is limited to some nematode species. to observe immunofluorescent localization of ifs in helminth tissues, we selected a murine hybridoma clone producing igm antibody to multiple types of mammalian if proteins and examined cross-reactivity to helminth proteins. the selected mo ... | 2000 | 10958445 |
| comparative evaluation of an indirect elisa test for diagnosis of swine cysticercosis employing antigen from taenia solium and taenia crassiceps metacestodes. | taenia solium cysticercosis is still a serious public health problem in several countries where poverty and lack of hygiene favor transmission. because pigs are the primary intermediate hosts, prevalence of porcine cysticercosis is a reliable indicator of active transmission zones. serological diagnostic methods are important tools for epidemiological studies since they can be applied to living animals on a large scale. four antigen preparations (cyst fluid and crude) from t. solium and t. crass ... | 2000 | 11035231 |
| sex steroids and parasitism: taenia crassiceps cisticercus metabolizes exogenous androstenedione to testosterone in vitro. | sex hormones are known to modulate immune responses and may be implicated in sex associated susceptibilities to infections. taenia crassiceps cysticerci grow to larger numbers in female mice than in males. gonadectomy alters the course of this infection and hormone replacement with 17beta-estradiol increases the parasite numbers. however, in chronic taenia crassiceps cysticercosis the sex-hormone profile of males becomes more like that of the females' and progressively loose their sexual behavio ... | 2000 | 11086233 |
| mitochondrial genetic code in cestodes. | the flatworm mitochondrial genetic code, which has been used for all species of the platyhelminthes, is mainly characterized by aua codon for isoleucine, aaa codon for asparagine and uaa codon for tyrosine. in eight species of cestodes (echinococcus multilocularis, echinococcus granlosus, taenia solium taenia saginata, taenia hydatigena, taenia crassiceps, hymenolepis nama and mesocestoides corti), the cytochrome c oxidase subunit i (coi) genes were partially sequenced to verify this genetic cod ... | 2000 | 11163447 |
| two epitopes shared by taenia crassiceps and taenia solium confer protection against murine t. crassiceps cysticercosis along with a prominent t1 response. | taenia crassiceps recombinant antigens ketc1 and ketc12 have been shown to induce high level of protection against experimental murine t. crassiceps cysticercosis, an experimental model successfully used to test candidate antigens for use in vaccination against porcine taenia solium cysticercosis. based on the deduced amino acid sequence, ketc1 and ketc12 were chemically synthesized in linear form. immunization with ketc1 induced 66.7 to 100% protection against murine cysticercosis, and immuniza ... | 2001 | 11179354 |
| parasite-altered host behavior in the face of a predator: manipulation or not? | parasitologists have generally accepted the idea that parasite-induced alterations in host behavior increase the chance for parasite survival and transmission or ensure the completion of its life cycle. the aim of the present study was to investigate modifications in the behavior of taenia crassiceps-infected balb/c mice in the face of a predator. the experiments showed modifications in the response of infected mice in comparison with uninfected controls on exposure to a predator final host. how ... | 2001 | 11293565 |
| detection of igg in cerebrospinal fluid for diagnosis of neurocysticercosis: evaluation of saline and sds extracts from taenia solium and taenia crassiceps metacestodes by elisa and immunoblot assay. | we compared saline (s) and sodium dodecyl sulphate (sds) extracts from taenia solium (homologous species - ho) and taenia crassiceps (heterologous species - he) metacestodes in order to detect igg by elisa and immunoblot assay (iba) in cerebrospinal fluid (csf) for the diagnosis of human neurocysticercosis (nc). csf samples were obtained from 93 patients. of these, 40 had nc, five had a diagnosis of probable nc, nine had central nervous system schistosomiasis or strongyloidiasis and 39 had other ... | 2001 | 11299039 |
| taenia crassiceps metacestodes have cytochrome oxidase aa3 but not cytochrome o functioning as terminal oxidase. | in mitochondria obtained from taenia crassiceps metacestodes, carbon monoxide difference spectra reveal signals characteristic of the classical mitochondrial oxidase, cytochrome aa3, as well as signals suggesting the presence of 'cytochrome o'. in the present work, using photodissociation spectrophotometry and analysis of the haem groups, we conclude that there is no haem o in these larvae, and that the only cytochrome that functions as terminal oxidase is cytochrome c oxidase, aa3. at temperatu ... | 2001 | 11356519 |
| heat shock and stress response of taenia solium and t. crassiceps (cestoda). | heat shock and stress responses are documented for the first time in larval stages of the cestodes taenia solium and taenia crassiceps. radioactive metabolic labelling after in vitro incubation of cysts at 43 degrees c, revealed the induction of heat shock proteins. in t. crassiceps, the major heat shock proteins were 80, 70 and 60 kda. after prolonged incubation, a set of low molecular weight heat shock proteins (27, 31, 33 and 38 kda), were also induced. in vitro incubation of cysts at 4 degre ... | 2001 | 11393832 |
| intraspleen dna inoculation elicits protective cellular immune responses. | dna immunization or inoculation is a recent vaccination method that induces both humoral and cellular immune responses in a range of hosts. independent of the route or site of vaccination, the transfer of antigen-presenting cells (apc) or antigens into lymphoid organs is necessary. the aim of this investigation was to test whether intraspleen (i.s.) dna inoculation is capable of inducing a protective immune response. we immunized mice by a single i.s. injection of a dna construct expressing the ... | 2001 | 11403718 |
| inhibitory role of antibodies in the development of taenia solium and taenia crassiceps toward reproductive and pathogenic stages. | untreated taenia solium cysticerci obtained from different naturally infected pigs vary notably in their capacity to develop into intestinal tapeworms in prednisolone-treated hamsters, whereas cells derived from taenia crassiceps cysticerci after 2 mo of infection almost always develop to cysticerci in the peritoneal cavity of susceptible balb/cann mice. preincubation of whole cysticerci or parasite cells with mice immunoglobulins raised against an 18-mer peptide epitope (gk-1) common to both pa ... | 2001 | 11426721 |
| kinetics and characterization of cellular responses in the peritoneal cavity of mice infected with taenia crassiceps. | changes in the leukocyte population of the peritoneal cavity ensue immediately after infection with taenia crassiceps metacestodes. basophils and neutrophils decrease, whereas macrophages, monocytes, and lymphocytes increase to reach only modest levels by 6 wk and then diminish to nearly disappear by 15 wk when the parasite begins rapid reproduction. eosinophils also appear early in infection, but then abate to lower levels that persist. in late infections, when the mass of cysticerci equals tha ... | 2001 | 11426723 |
| characterization and protective potential of the immune response to taenia solium paramyosin in a murine model of cysticercosis. | paramyosin has been proposed as a vaccine candidate in schistosomiasis and filariasis. however, limited information is available about its protective potential against cysticercosis and the immune response it induces. immunization of mice with recombinant full-length paramyosin of taenia solium (tpmy) results in about a 52% reduction in parasite burden after a subsequent challenge by intraperitoneal inoculation of taenia crassiceps cysticerci. immunization assays using recombinant fragments of t ... | 2001 | 11500411 |
| cysticercus antigens in cerebrospinal fluid samples from patients with neurocysticercosis. | antigens were detected in cerebrospinal fluid (csf) samples from patients with neurocysticercosis (nc) by enzyme-linked immunosorbent assay (elisa) using polyclonal sera of rabbit anti-taenia solium cysticerci (anti-tso) and anti- taenia crassiceps cysticerci vesicular fluid (anti-tcra or anti-tcra <30 kda). a group of nc patients (n = 174) were studied (nc), including 40 patients in different phases of the disease. elisas carried out with the anti-tso, anti-tcra, and anti-tcra <30 kda showed se ... | 2001 | 11526181 |
| synthetic peptide vaccine against taenia solium pig cysticercosis: successful vaccination in a controlled field trial in rural mexico. | taenia solium cysticercosis seriously affects human health when localised in the central nervous system (cns) and causes great economic loss in pig husbandry in rural areas of endemic countries. increasing the resistance to the parasite in the obligatory host pig may help in curbing transmission. three synthetic peptides based on protein sequences of the murine parasite taenia crassiceps, which had previously been shown to induce protection in mice against homologous challenge, were tested as a ... | 2001 | 11567772 |
| immunoendocrine interactions during chronic cysticercosis determine male mouse feminization: role of il-6. | taenia crassiceps cysticercosis results in an impressive feminization in male mice during chronic infection, characterized by increased serum estradiol levels 100 times their normal values, while those of testosterone and dihydrotestosterone are decreased by 85 and 95% respectively. concomitantly, the levels of follicle-stimulating hormone and il-6 are increased 70 and 90 times their normal values in the infected male mice. since a specific th1/th2 shift of the immune response has been previousl ... | 2001 | 11591780 |
| serodiagnosis of human cysticercosis by using antigens from vesicular fluid of taenia crassiceps cysticerci. | neurocysticercosis (nc), caused by the presence of taenia solium metacestodes in tissues, is a severe parasitic infection of the central nervous system with universal distribution. to determine the efficiency of enzyme-linked immunosorbent assay (elisa) and immunoblot with antigens of t. crassiceps vesicular fluid (tcra) compared to standard techniques (indirect immunofluorescence test [ift] and complement fixation test [cft]) using t. solium cysticerci (tso) for the serodiagnosis of nc, we stud ... | 2001 | 11687454 |
| cd4+ tcralphabeta t cells are critically involved in the control of experimental murine cysticercosis in c57bl/6j mice. | taenia crassiceps cysticerci develop in the peritoneal cavity of balb/cann mice and, to a lesser extent, in c57bl/6j mice. the mechanisms involved in the immunity to this murine cysticercosis seem to be mainly mediated by t cells. to gain further insight into the mechanisms of cysticercal immunity, the susceptibility of mice deficient in different immunologically relevant genes was compared with that of the respective wild type. mice were classified according to the parasite load and survival af ... | 2001 | 11688888 |
| effect of oral zinc supplementation upon taenia crassiceps murine cysticercosis. | the effect of zinc supplementation on taenia crassiceps murine cysticercosis was studied in susceptible balb/cann mice. female offspring of mice supplemented with high zinc throughout gestation and lactation were intraperitoneally infected with t. crassiceps cysticerci. offspring from nonsupplemented mothers were used as controls. significantly fewer parasites were recovered from zinc-supplemented mice (zsm) 30 days after infection. increased resistance was not related to the igg antibody respon ... | 2001 | 11695361 |
| antigen-specific suppression of cultured lymphocytes from patients with neurocysticercosis. | the biological parasite-host interactions involved in neurocysticercosis (nc) are of a complex nature. a lymphoproliferation assay was performed using mononuclear cells from 11 patients with nc, who were classified according to the alterations obtained by imaging examinations. antigen extracts from the membrane and/or scolex of taenia solium and from the vesicular fluid of taenia crassiceps were used. mononuclear cells from patients with nc showed antigen-specific suppression when compared with ... | 2001 | 11703375 |
| use of taenia crassiceps cysticercus antigen preparations for detection of antibodies in cerebrospinal fluid samples from patients with neurocysticercosis (taenia solium). | antigen extracts obtained from the vesicular fluid of taenia crassiceps cysticerci and from fractions purified by affinity chromatography with the lectin concanavalin a and the glycoprotein antigen separated by electrophoresis were used for the detection of taenia solium anticysticercus antibodies. the sensitivity and specificity obtained for all antigens were 100% in enzyme-linked immunosorbent assay with good reproducibility. using immunoblotting of the three antigens, low-molecular-mass pepti ... | 2002 | 11777854 |
| complete mitochondrial genomes confirm the distinctiveness of the horse-dog and sheep-dog strains of echinococcus granulosus. | unlike other members of the genus, echinococcus granulosus is known to exhibit considerable levels of variation in biology, physiology and molecular genetics. indeed, some of the taxa regarded as 'genotypes' within e. granulosus might be sufficiently distinct as to merit specific status. here, complete mitochondrial genomes are presented of 2 genotypes of e. granulosus (g1-sheep-dog strain: g4-horse-dog strain) and of another taeniid cestode, taenia crassiceps. these genomes are characterized an ... | 2002 | 11811807 |
| [immunodiagnosis of neurocysticercosis: comparative study of antigenic extracts from cysticercus cellulosae and taenia crassiceps]. | different antigenic extracts of taenia solium and taenia crassiceps were evaluated in connection with the detection of antibodies in patients with neurocysticercosis aimed at selecting immunorelevant antigens for the diagnosis of neurocysticercosis by means of the immunoenzymatic assay and immunoblotting. the vesicular fluid of t. crassiceps proved to be more sensitive (100%) and specific (86%). on using the immunoblotting technique it was also observed that this extract was the most sensitive a ... | 2000 | 11826516 |
| frequency of serum anti-cysticercus antibodies in the population of a rural brazilian community (cássia dos coqueiros, sp) determined by elisa and immunoblotting using taenia crassiceps antigens. | considering the impact of cysticercosis on public health, especially the neurologic form of the disease, neurocysticercosis (nc), we studied the frequency of positivity of anti-taenia solium cysticercus antibodies in serum samples from 1,863 inhabitants of cássia dos coqueiros, sp, a municipal district located 80 km from ribeirão preto, an area considered endemic for cysticercosis. the 1,863 samples were tested by enzyme linked immunosorbent assay (elisa) using an antigenic extract from taenia c ... | 2002 | 11896406 |
| cutting edge: susceptibility to the larval stage of the helminth parasite taenia crassiceps is mediated by th2 response induced via stat6 signaling. | using stat6(-/-) balb/c mice, we analyzed the role of stat6-induced th2 response in determining the outcome of murine cysticercosis caused by the helminth parasite taenia crassiceps. after t. crassiceps infection, wild-type balb/c mice developed a strong th2-like response; produced high levels of igg1, ige, il-4, as well as il-13; and remained susceptible to t. crassiceps. in contrast, similarly infected stat6(-/-) mice mounted a strong th1-like response; produced high levels of igg2a, il-12, if ... | 2002 | 11907063 |
| larval taenia crassiceps secretes a protein with characteristics of murine interferon-gamma. | previous studies have shown that excretory/secretory products of larval taenia crassiceps have immunomodulatory activities. we report here that one of these products, termed p66, possesses activities that mimic some characteristics of murine interferon-gamma (ifn-gamma). purified p66 cross-reacts with anti-murine ifn-gamma on immunoblots and increases concanavalin-a-induced splenic t-cell proliferative responses of normal and chronically infected mice. it has also been shown that p66 induces enh ... | 2002 | 12049460 |
| immunoblot analysis using antigen from taenia crassiceps cysticerci in the diagnosis of swine cysticercosis. | 2001 | 12058673 | |
| chronic helminth infection induces alternatively activated macrophages expressing high levels of ccr5 with low interleukin-12 production and th2-biasing ability. | helminth infections induce th2-type biased immune responses. although the mechanisms involved in this phenomenon are not yet clearly defined, antigen-presenting cells (apc) could play an important role in this process. here, we have used peritoneal macrophages (f4/80+) recruited at different times after challenge with taenia crassiceps as apc and tested their ability to regulate th1/th2 differentiation. macrophages from acute infections produced high levels of interleukin-12 (il-12) and nitric o ... | 2002 | 12065507 |
| suppressed cytotoxic t lymphocyte responses in experimental cysticercosis. | mitogen-induced t cell responses are suppressed in mice infected with larvae of taenia crassiceps. the effects of experimental infection on specific t cell responses, however, have not been examined. in the present study, we demonstrate that larval-infected mice exhibit suppressed ability to develop anti-virus specific cytotoxic t lymphocyte (ctl) responses while maintaining apparently normal natural killer (nk) cell responsiveness. | 2002 | 12079738 |
| evaluation of an antigen from taenia crassiceps cysticercus for the serodiagnosis of neurocysticercosis. | we report here the evaluation of an antigen from taenia crassiceps cysticercus as a potential reagent in an enzyme-immunoelectrotransfer blotting assay (eitb) and an enzyme-linked immunosorbent assay (elisa) for the serodiagnosis of neurocysticercosis (nc) using clinical specimens obtained from patients in different phases of the disease. serum and cerebrospinal fluid (csf) samples from 64 patients suspected of having nc according to clinical manifestation and brain computed tomography were test ... | 2002 | 12088857 |
| taenia crassiceps: androgen reconstitution of the host leads to protection during cysticercosis. | the effects of testosterone, dihydrotestosterone, and 17beta-estradiol in castrated mice of both sexes infected with taenia crassiceps cysticerci were studied. the results showed that castration and treatment with either testosterone or dihydrotestosterone before infection decreased parasite loads by 50 and 70%, respectively, while the treatment with 17beta-estradiol increased it by three times in both genders, as compared with control mice. the specific splenocyte cell proliferation and il-2 an ... | 2002 | 12128047 |
| excretory/secretory antigens (es) from in-vitro cultures of taenia crassiceps cysticerci, and use of an anti-es monoclonal antibody for antigen detection in samples of cerebrospinal fluid from patients with neurocysticercosis. | antigens were obtained from cysticerci of the orf strain of taenia crassiceps, by culture of cysts in protein-free hybridoma medium (pfhm). budding of new vesicles was observed after 24-48 h. excretory/secretory (es) antigens (peptides of <20 kda) were recovered in the medium after culture for 48 h. sds-page analysis of vesicular-fluid (vf) antigens (obtained by rupturing t. crassiceps cysticerci in pfhm) and the es antigens indicated partial homology between the two preparations. es peptides of ... | 2002 | 12171617 |
| castration and pregnancy of rural pigs significantly increase the prevalence of naturally acquired taenia solium cysticercosis. | cuentepec is a rural village of central mexico, where 1300 pigs were bred at the time of the study in conditions that favor taenia solium transmission. the tongues of 1087 (84%) of these pigs were visually examined and 33% were found to be cysticercotic. castration of male pigs increased prevalence from 23 to 50% (p < 0.001) and pregnancy in sows also increased their prevalence from 28 to 59% (p < 0.001). thus, endocrinological conditions characterized by low levels of androgens or high levels o ... | 2002 | 12191898 |
| do interleukin-6 and macrophage-migration inhibitory factor play a role during sex-associated susceptibility in murine cysticercosis? | in murine taenia crassiceps cysticercosis, females sustain larger intensities of infection than males. however, during chronic infection, this difference disappears and males show a feminization process. to further study the role of two cytokines, interleukin-6 (il-6) and macrophage-migration inhibitory factor (mif), known to be involved in immunoendocrinological processes during sex-associated susceptibility in cysticercosis, il-6 and mif gene knockout (ko) mice were infected, and the number of ... | 2002 | 12209330 |
| behavioural dominance and taenia crassiceps parasitism in balb/c male mice. | behavioural dominance relationships in mouse populations are based upon fighting and antagonistic behaviour. social rank is affected by the physiological states present in the mice. experimental infection by taenia crassiceps cysticerci induced physiological disorders and disrupted the dominant-subordinate status. infected male mice showed strong perturbations in territorial behaviour and aggressiveness. infected dominant male mice did not show a significant reversal of dominance order compared ... | 2002 | 12209332 |
| cerebral cysticercosis in a woodchuck (marmota monax). | a juvenile woodchuck (marmota monax) with vestibular signs was found in woodbridge, ontario (canada) and later euthanized. at necropsy there was marked distortion of the right side of the skull, where a large, fluctuant, subcutaneous mass extended under the zygomatic arch and caudally from the right eye towards the right ear. the mass was multiloculated and contained a large number of tapeworm cysticerci, each about 1 to 2 mm in diameter. the third and lateral ventricles of the brain were dilate ... | 2002 | 12238384 |
| identification of echinococcus granulosus eggs. | the eggs from echinococcus granulosus contaminate the environment spreading out the disease among the herbivorous. the differential diagnosis of the embriophores recovered from the soil is very difficult by morphologic and immunologic methods. in this paper we evaluate the ego/dna-im1 for identification of e. granulosus oncosphere dna and differentiation of eggs from other taeniid. the positive result of the pcr technique shows an amplification fragment of the expected size (285 bp) correspondin ... | 2002 | 12376028 |
| induction of immunoglobulin g1, interleukin-6 and interleukin-10 by taenia crassiceps metacestode carbohydrates. | t helper type 2 (th2) -polarized immune responses are characteristically dominant in helminth infections. two murine models that show a th1 to th2 polarization with infection progression are those of schistosoma mansoni and taenia crassiceps. in both, an early th1 response is replaced by a late th2 response. we report that the nucleic acid-, protein- and lipid-free carbohydrate fraction of t. crassiceps metacestodes (denoted t-cho) possesses th2-like immunomodulatory activity. immunization of tw ... | 2002 | 12460185 |
| helminth fauna of the red fox (vulpes vulpes linnaeus, 1758) in southern belarus. | we examined 94 carcasses of and 1,213 faecal samples from red foxes collected between 1981 and 2001 in natural and transformed ecosystems in the southern part of belarus. a total of 32 helminth species were found. of these, the most common were alaria alata, pearsonema plica, taenia crassiceps, toxocara canis, trichinella spp. larvae and uncinaria stenocephala. all species are significant for medical and veterinary health. | 2003 | 12474048 |
| protective immunity against taenia crassiceps murine cysticercosis induced by dna vaccination with a taenia saginata tegument antigen. | this study investigated the protective capacity of the recombinant taenia saginata tso18 antigen administered as a dna vaccine in the taenia crassiceps murine model of cysticercosis. this tso18 dna sequence, isolated from a t. saginata oncosphere cdna library, has homologies with taenia solium and echinococcus sp. it was cloned in the pcdna3.1 plasmid and injected once intramuscularly into mice. compared to saline-vaccinated control mice, immunization reduced the parasite burden by 57.3-81.4%, w ... | 2002 | 12475632 |
| characterization of cdna encoding a l37a ribosomal protein from taenia crassiceps and its potential use in phylogenetic reconstructions. | in this study, we characterized for the first time the complete sequence of a l37a cdna from a cestode specie: taenia crassiceps. a phylogenetic analysis of l37a ribosomal proteins from distant animal species is presented and the potential use of such proteins in molecule-based phylogeny is discussed. | 2002 | 12594966 |
| macrophage migration inhibitory factor plays a critical role in mediating protection against the helminth parasite taenia crassiceps. | to determine the role of endogenous migration inhibitory factor (mif) in regulation of immune response during murine cysticercosis caused by the helminth parasite taenia crassiceps, we analyzed the course of t. crassiceps infection in mif(-/-) balb/c mice. mif(-/-) mice were highly susceptible to t. crassiceps and developed significantly higher parasite loads compared to similarly infected mif(+/+) mice. throughout the course of infection, taenia crassiceps soluble antigen-stimulated spleen cell ... | 2003 | 12595439 |
| the initial immune response during experimental cysticercosis is of the mixed th1/th2 type. | the immunological events that occur during the initial stages of experimental cysticercosis are not known. the studies presented here examined the cytokines produced by peritoneal exudate cells (pecs), splenocytes and mesenteric lymph node (mln) cells during the first week of infection with larval taenia crassiceps in balb/cj mice. proliferation assays determined that the earliest time when antigen-specific responses could be measured was 5 days post-infection. concanavalin a (cona) stimulation ... | 2003 | 12632156 |
| cd4+ and cd19+ splenocytes undergo apoptosis during an experimental murine infection with taenia crassiceps. | the taenia crassiceps cysticercus is a cestode that naturally and experimentally infects rodents in which it reproduces by budding. in the laboratory, a persistent cellular immunosuppression with a concomitant increasing load of parasites has been observed in experimentally infected balb/cann mice. in this study, enhanced apoptosis was found in spleen cells from 30-day infected mice with a typical "ladder-patterned" dna fragmentation and an increase in phosphatidylserine expression. a characteri ... | 2003 | 12756553 |
| a comparative study of biochemical and immunological properties of triosephosphate isomerase from taenia solium and sus scrofa. | we produced the taenia solium triosephosphate isomerase (tpi) in escherichia coli and compared its biochemical and immunological properties with those of the commercial tpi from sus scrofa. taenia solium tpi is a homodimer composed of two 27-kda monomers, with a specific activity of 5,683 u/mg and a km value of 0.758, and s. scrofa tpi is also dimeric with similar monomeric molecular weight, specific activity of 4,227 u/mg, and a km value of 0.51. the catalytic parameters for the isomerization o ... | 2003 | 12760631 |
| taenia crassiceps cysticercosis: immune response in susceptible and resistant balb/c mouse substrains. | taenia crassiceps can naturally and experimentally infect rodents in which they reproduce by budding. differences in the susceptibility to t. crassiceps cysticercosis were found between two balb/c substrains: balb/cann (susceptible) and balb/cj (resistant). in chimeric mice, resistance was transferred to susceptible mice with bone marrow cells from the resistant mice, which argues in favor of an immune mediation of the resistant phenotype. to further explore the immune response that could underl ... | 2003 | 12783314 |
| altered t helper responses in cd40 and interleukin-12 deficient mice reveal a critical role for th1 responses in eliminating the helminth parasite taenia crassiceps. | a key feature of helminth infections is the induction of strong th2-biased immune responses in their hosts. we have previously found that th2-like responses mediate susceptibility to the helminth parasite taenia crassiceps, probably by inhibiting th1 responses required for the development of protective immunity against this parasite. here we show that mice lacking interleukin-12p35 (il-12p35-/-) following t. crassiceps infection, failed to mount a th1 response, but developed a strong th2-type re ... | 2003 | 12814650 |
| taenia crassiceps cysticercosis in mice does not increase the carcinogenic effect of methyl-nitrosourea. | an association between brain cysticercosis and malignant neoplasms in humans has recently been reported. to explore the possibility of a potentiating effect of cysticercosis on carcinogenesis mice infected with taenia crassiceps cysticerci were exposed to the carcinogenic substance methyl-nitrosourea; 35% of them developed lymphoma, in contrast with 50% of control non-infected animals exposed to mnu. in this experimental model of cysticercosis we did not find a potentiating effect of peritoneal ... | 2003 | 12880594 |
| steroid hormone production by parasites: the case of taenia crassiceps and taenia solium cysticerci. | many examples of reciprocal endocrine interactions between parasites and hosts have been found in insects, arthropods and mammals. cysticercosis produced by taenia solium metacestodes is a widely distributed parasite infection that affects the human and the pig. taenia crassiceps experimental murine cysticercosis has been used to explore the role of biological factors involved in host-parasite interactions. we had shown that t. crassiceps cysticercosis affects the serum concentration of steroid ... | 2003 | 12943707 |
| aldolase in the larval form of taenia crassiceps. | 1958 | 13537936 | |
| maintenance of larval taenia crassiceps (cestoda: cyclophyllidea) in a chemically defined medium. | 1963 | 14099841 | |
| cerebral cysticercosis by taenia crassiceps in a domestic cat. | cerebral cysticercosis by taenia crassiceps was diagnosed in an adult female domestic shorthair cat. the animal was euthanized 6 weeks after the initial presentation with signs of vomiting, lethargy, and ataxia. the disease took an intermittent relapsing course with the neurological signs progressing eventually to recumbancy and coma. at necropsy, numerous cysticerci were found in the dilated left lateral ventricle and the adjacent brain parenchyma. the cysticerci were identified as metacestodes ... | 2003 | 14535554 |
| epileptogenic activity of granulomas associated with murine cysticercosis. | neurocysticercosis, caused by taenia solium, is a common cause of neurologic disease in developing countries and among immigrants to the united states. seizures are the most common clinical manifestation of neurocysticercosis. imaging studies of patients with seizures from neurocysticercosis typically reveal evidence of an inflammatory reaction associated with the parasite or calcified granulomas. this study investigated whether a substance produced by the host granulomatous reaction to the dyin ... | 2003 | 14552894 |
| population genetic structure of taenia solium from madagascar and mexico: implications for clinical profile diversity and immunological technology. | taenia solium is a cestode parasitic of humans and pigs that strongly impacts on public health in developing countries. its larvae (cysticercus) lodge in the brain, causing neurocysticercosis, and in other tissues, like skeletal muscle and subcutaneous space, causing extraneuronal cysticercosis. prevalences of these two clinical manifestations vary greatly among continents. also, neurocysticercosis may be clinically heterogeneous, ranging from asymptomatic forms to severely incapacitating and ev ... | 2003 | 14572511 |
| helminth antigens (taenia solium, taenia crassiceps, toxocara canis, schistosoma mansoni and echinococcus granulosus) and cross-reactivities in human infections and immunized animals. | helminth antigens were investigated in the search for accessible heterologous antigens capable to discriminate different helminthiases, by the enzyme linked immunosorbent assay (elisa) and the immunoblot assay (ib). antigens used were: taenia solium cysticercus total saline (tso); taenia crassiceps cysticercus vesicular fluid (tcra-vf); t. crassiceps cysticercus glycoproteins (tcra-gp and tcra-(18-14)-gp); toxocara canis larva excretory-secretory (tes); schistosoma mansoni adult total saline (sm ... | 2003 | 14636985 |
| the parasitic trematode fasciola hepatica exhibits mammalian-type glycolipids as well as gal(beta1-6)gal-terminating glycolipids that account for cestode serological cross-reactivity. | neutral glycosphingolipids from sheep-derived fasciola hepatica liver flukes were isolated and characterized both structurally and serologically. after hplc fractionation, glycolipids were analyzed by linkage analysis, enzymatic cleavage, and maldi-tof as well as electrospray ionization mass spectrometry. obtained results revealed the presence of two types of neutral glycolipids. the first group represented mammalian-type species comprising globo- and isoglobotriaosylceramides (gal(alpha1-4)gal( ... | 2004 | 14638629 |
| purification, characterization and kinetic properties of the multifunctional thioredoxin-glutathione reductase from taenia crassiceps metacestode (cysticerci). | the multifunctional enzyme thioredoxin-glutathione reductase (tgr) was purified to homogeneity from the soluble fraction of taenia crassiceps metacestode (cysticerci). specific activities of 17.5 and 4.7 u mg(-1) were obtained with plasmodium falciparum thioredoxin and gssg, respectively, at ph 7.75. under the same conditions, km values of 17, 15, and 3 microm were respectively calculated for thioredoxin, gssg and nadph. the kcat/km ratio of t. crassiceps tgr for both thioredoxin and gssg falls ... | 2004 | 14668013 |
| infrapopulation dynamics of a wild strain of taenia crassiceps (wfu) (cestoda: taeniidae) in balb/cj mice. | taenia crassiceps cysticerci form large infrapopulations that persist in the tissues of their rodent hosts. early infrapopulation growth appears inhibited and is followed by rapid increases that appear not to be controlled by the host immune response. this investigation was undertaken to examine the infrapopulation growth dynamics of a normally developing strain (wfu) of t. crassiceps during a 60-day primary intraperitoneal (i.p.) infection. three, 6, 9, 14, 28, and 60 days after i.p. inoculatio ... | 2004 | 15040670 |
| remote sensing of intraperitoneal parasitism by the host's brain: regional changes of c-fos gene expression in the brain of feminized cysticercotic male mice. | experimental intraperitoneal taenia crassiceps cysticercosis in mice exhibits distinct genetical, immunological and endocrinological features possibly resulting from the complex interactive network of their physiological systems. very notable is the tendency of parasites to grow faster in hosts of the female sex. it is also remarkable in the feminization process that the infection induces in chronically infected male mice, characterized by their estrogenization, deandrogenization and loss of sex ... | 2004 | 15074883 |
| ultrastructure of spermiogenesis and the spermatozoon in taenia crassiceps strobilae wfu strain (cestoda, cyclophyllidea, taeniidae) from golden hamsters. | strobilae from taenia crassiceps (wfu strain) were obtained from outbred hamsters (mesocricetus auratus) by feeding them viable metacestodes maintained by intraperitoneal passage in female balb/c mice. mature and gravid proglottids from strobilae were recovered from hamster intestines and fixed for light and electron microscopy. by light microscopy, the expected structure of taeniid proglottids was observed. ultrastructural analysis of ten proglottids showed that testicular follicles and vas def ... | 2004 | 15146328 |
| in vitro effects of albendazole sulfoxide and praziquantel against taenia solium and taenia crassiceps cysts. | we investigated the minimum exposure times of prazicuantel (pzq) and albendazole sulfoxide (abzso) required for their activities against taenia cysts in vitro as well as the 50 and 99% effective concentrations. the results showed that although the effects of both drugs are time and concentration dependent, abzso acts much slower and is less potent than pzq. | 2004 | 15155240 |
| [toward development of a taenia solium paramyosin-based vaccine against porcine cysticercosis]. | taenia solium paramyosin (tpmy) is a prominent 100 kda antigen in human and porcine cysticercosis. tpmy is an alpha-helical coiled coil protein present in muscle and tegumentary structures of t. solium cysticerci. tpmy has the property of binding c1q resulting in inhibition of the complement cascade. tpmy probably binds c1q through its collagen-like domains and could be involved in a parasite strategy to modulate host immune response. humoral immune response against tpmy is prefrentially directe ... | 2004 | 15162945 |
| taenia crassiceps carbohydrates stimulate il-6 expression in naïve murine macrophages via toll-like receptors (tlrs). | in murine infections of schistosoma mansoni and taenia crassiceps, a characteristic polarization of the immune response from a th-1 type to a th-2 type occurs with progression of infection. in s. mansoni, egg glycoprotein carbohydrates are said to be responsible for this immunomodulatory activity. we have previously shown that in vitro systems, t. crassiceps carbohydrates (tcho) up-regulate the expression of interleukin 6 (il-6) in naïve spleen cells, while conventional th-2 cytokines were not d ... | 2004 | 15163536 |
| cellular immune response of patients with neurocysticercosis (inflammatory and non-inflammatory phases). | the cellular immune response in neurocysticercosis (nc) was studied in 22 patients, 11 (50%) of them in the inflammatory phase of the disease, by means of immunophenotyping of cells in cerebrospinal fluid (csf) and peripheral blood (pb), lymphoproliferation assay with taenia solium total saline extract (tso) and taenia crassiceps vesicular fluid (tcra) as stimuli, and by determining the cytokine production profile in the cell culture supernatant. a higher mean percentage of cd19+ and cd56+ cells ... | 2004 | 15234670 |
| a stat4-dependent th1 response is required for resistance to the helminth parasite taenia crassiceps. | to determine the role of stat4-dependent th1 responses in the regulation of immunity to the helminth parasite taenia crassiceps, we monitored infections with this parasite in resistant mice lacking the stat4 gene. while t. crassiceps-infected stat4(+/+) mice rapidly resolved the infection, stat4(-/-) mice were highly susceptible to infection and displayed large parasite loads. moreover, the inability of stat4(-/-) mice to control the infection was associated with the induction of an antigen-spec ... | 2004 | 15271915 |
| differential expression of ap-1 transcription factor genes c-fos and c-jun in the helminth parasites taenia crassiceps and taenia solium. | homologues of c-fos and c-jun from total dna of taenia crassiceps and taenia solium were cloned and sequenced. the amino acid alignment analysis revealed that c-fos dnas from t. crassiceps and t. solium were highly homologous (96%), and both have high homology compared to several mammalian c-fos proteins (93% to mouse, 96% to rat and 86% to human). the c-jun protein alignment showed higher homology (t. crassiceps and t. solium have 98%), when compared with mouse, rat and human, being 92%, 98% an ... | 2004 | 15376782 |
| taenia antigens detection in the cerebrospinal fluid of patients with neurocysticercosis and its relationship with clinical activity of the disease. | (1) to determine the concentration of taenia antigens in the cerebrospinal fluid (csf) of patients with neurocysticercosis (nc); (2) to establish its relationship with clinical activity of the disease and with classical variables of csf. | 2004 | 15476063 |
| macrophage galactose-type c-type lectins as novel markers for alternatively activated macrophages elicited by parasitic infections and allergic airway inflammation. | molecular markers, especially surface markers associated with type ii, cytokine-dependent, alternatively activated macrophages (aamf), remain scarce. besides the earlier documented markers, macrophage mannose receptor and arginase 1, we demonstrated recently that murine aamf are characterized by increased expression of found in inflammatory zone 1 (fizz1) and the secretory lectin ym. we now document that expression of the two members of the mouse macrophage galactose-type c-type lectin gene fami ... | 2004 | 15591125 |
| [seroepidemiological survey of human cysticercosis in a municipality of piauí state, northeast brazil]. | as part of parasitological studies in the area surrounding the serra da capivara national park, piauí state, northeast brazil, from 1999 to 2001, the current study aimed to evaluate the epidemiological profile of human cysticercosis in the municipality of joão costa. clinical and epidemiological data were obtained, and blood samples were drawn for immunoenzymatic serological tests (elisa and western blot), using taenia crassiceps as the antigen. the first stage, in 1999, investigated 169 individ ... | 2004 | 15608856 |
| molecular mechanisms involved in the differential effects of sex steroids on the reproduction and infectivity of taenia crassiceps. | the in vitro exposure of taenia crassiceps cysticerci to 17-beta estradiol (e2) and progesterone (p4) stimulated their reproduction and infectivity. testosterone (t4) and dihydrotestosterone (dht) inhibited their reproduction and reduced their motility and infectivity. e2 and p4 increased, whereas t4 and dht reduced, the expression of parasite c-fos and c-jun and dna synthesis. in vitro exposure of cysticerci to sex steroids before their inoculation into recipient noninfected mice resulted in la ... | 2004 | 15715212 |
| genetic vaccination against murine cysticercosis by using a plasmid vector carrying taenia solium paramyosin. | a plasmid vector carrying the immunoprotective amino-terminal fragment of taenia solium paramyosin (vw2-1) was designed for genetic vaccination studies. mice that were genetically immunized with vw2-1 and challenged by intraperitoneal inoculation of taenia crassiceps cysticerci showed 43 to 48% reductions in the parasite burden, values which were similar to values obtained previously when the recombinant protein was used. | 2005 | 15731096 |
| apoptosis patterns in experimental taenia solium and taenia crassiceps strobilae from golden hamsters. | apoptosis or programmed cell death (pcd) patterns of two taeniid species, taenia solium and taenia crassiceps, were explored in adult tapeworms grown in golden hamsters. animals were fed either ten viable t. solium cysticerci from naturally infected pigs or from t. crassiceps wfu strain maintained in balb/c mice. adult strobilae were recovered from the intestine at different times after infection and either frozen at -70 degrees c or fixed in paraformaldehyde-glutaraldehyde. frozen sections were ... | 2005 | 15759155 |
| regulation of the immune response to cestode infection by progesterone is due to its metabolism to estradiol. | the aim of this work was to investigate the role of progesterone during taenia crassiceps cysticercosis, and the immunological mechanisms involved in its effects, by relating progesterone treatment to whole parasite counts, to host humoral and cellular immune response, to the presence or absence of nuclear receptors to sex steroids in splenocytes, and to serum sex steroid levels in infected mice of both genders. progesterone treatment increased parasite loads two-fold in females and three-fold i ... | 2005 | 15804489 |
| reactive oxygen species and 12/15-lipoxygenase contribute to the antiproliferative capacity of alternatively activated myeloid cells elicited during helminth infection. | understanding the role of cd11b(+)gr-1(+) myeloid suppressor cells in the immune suppression and immunoregulation associated with a variety of diseases may provide therapeutic opportunities. in this article, we show, in a model of helminth infection, that cd11b(+)gr-1(+) myeloid suppressor cells but not cd11b(+)f4/80(high) mature macrophages expanded in the peritoneal cavity of balb/c mice implanted with taenia crassiceps. peritoneal cell populations from early stage-infected animals impaired t ... | 2005 | 15879104 |
| isolation of the taenia crassiceps antigens from a phage display cdna library and evaluation of their use for diagnosis of neurocysticercosis. | a novel cdna cloning strategy consisting in elimination of non-coding dna sequences from 3' regions of cdnas was applied to construct the taenia crassiceps phage displayed cdna expression library. after biopanning using immune sera, three phage clones expressing t. crassiceps-derived antigens specifically recognizing antibodies present in cerebrospinal fluid and plasma samples from neuroimaging-confirmed neurocysticercosis patients were selected. this novel cloning strategy may be applied to oth ... | 2005 | 15905129 |
| adjuvant effect of taenia crassiceps glycans against leishmanial antigens in mice infected with leishmania mexicana. | complex glycans derived from lipid, nucleic acid and protein free extracts of taenia crassiceps metacestode larvae were found to have adjuvant effect against leishmania mexicana antigens in balb/c mice experimentally infected with l. mexicana. a single intraperitoneal or subcutaneous injection of taenia glycans altered the th-1/th-2 balance in experimentally infected mice as determined by western blot analysis of igg 1 and igg 2a antibodies to l. mexicana antigens. leishmania antigens which were ... | 2005 | 15950742 |
| cyanide-resistant respiration in taenia crassiceps metacestode (cysticerci) is explained by the h2o2-producing side-reaction of respiratory complex i with o2. | the nature of the cyanide-resistant respiration of taenia crassiceps metacestode was studied. mitochondrial respiration with nadh as substrate was partially inhibited by rotenone, cyanide and antimycin in decreasing order of effectiveness. in contrast, respiration with succinate or ascorbate plus n,n,n',n'-tetramethyl-p-phenylenediamine (tmpd) was more sensitive to antimycin and cyanide. the saturation kinetics for o2 with nadh as substrate showed two components, which exhibited different oxygen ... | 2005 | 15958286 |
| cysticercosis immunodiagnosis using 18- and 14-kilodalton proteins from taenia crassiceps cysticercus antigens obtained by immunoaffinity chromatography. | monoclonal antibodies (mab) against taenia crassiceps and taenia solium cysticerci were produced and showed cross-reactivity with a 14-kda protein from t. solium and with 18- and 14-kda proteins from t. crassiceps. these mabs and antibodies from cerebrospinal fluid (csf) as well as serum samples from patients with neurocysticercosis (nc) reacted with 18- and 14-kda t. crassiceps proteins purified by immunoaffinity chromatography using a sepharose column coupled with mabs (anti-excretory/secretor ... | 2005 | 16000432 |
| mass spectrometric characterisation of taenia crassiceps metacestode n-glycans. | 2005 | 16024105 | |
| use of the paired samples (cerebrospinal fluid and serum) in immunodiagnostic of active and inactive human neurocysticercosis. | paired samples of cerebrospinal fluid (csf) and serum of 30 patients--10 with active, 10 with inactive neurocysticercosis (ncc), and 10 control subjects--were evaluated by enzyme-linked immunosorbent assay (elisa) using two taenia crassiceps metacestode extracts as antigen in order to detect igg antibodies. in active ncc, high levels of igg were detected (p < 0.05). the csf samples showed 80% (ci 72-88) of reactivity in the saline extract (s) and 90% (ci 84-95) in sodium dodecyl sulphate (sds) a ... | 2005 | 16113892 |
| role of the programmed death-1 pathway in the suppressive activity of alternatively activated macrophages in experimental cysticercosis. | we characterised a population of macrophages potentially involved in the immunoregulation induced by experimental cysticercosis. following taenia crassiceps infection, macrophages recruited in the peritoneal cavity were isolated and co-cultured at different ratios with t cells from naïve mice previously stimulated with anti-cd3/cd28 antibodies; these macrophages inhibited naïve t cell proliferation. this suppressive effect was interleukin (il)-10, interferon-gamma (ifn-gamma), and nitric oxide ( ... | 2005 | 16126211 |
| the role of sex steroids in the complex physiology of the host-parasite relationship: the case of the larval cestode of taenia crassiceps. | sex steroids play a significant role in regulating the parasite load in experimental intraperitoneal taenia crassiceps cysticercosis of male and female balbc/ann mice. briefly, oestrogens increase parasite loads and androgens decrease them (1) by acting directly on the parasite, favouring or hindering its reproduction, respectively, and (2) by biasing the hosts' immune response towards a parasite-permissive th2 or a parasite-restrictive th1 response. the infected male host also undergoes drastic ... | 2005 | 16178349 |
| purification and characterization of a metacestode cysteine proteinase from taenia solium involved in the breakdown of human igg. | infection of the central nervous system by taenia solium cysticerci is the cause of human neurocysticercosis, a major neurological infection in the third world and an emerging infectious disease in the united states. we previously isolated a cysteine proteinase from cysticerci of taenia crassiceps and demonstrated that it degrades human igg in vitro. we have now isolated a 48 kda thiol-dependent proteinase from t. solium. the t. solium enzyme also degrades human igg, but does not significantly d ... | 2005 | 16178363 |
| intact glycans from cestode antigens are involved in innate activation of myeloid suppressor cells. | during helminthic infections, strong th2 type-biased responses concomitant with impaired cell-proliferative responses to parasitic and unrelated antigens are major immunological hallmarks. parasite glycan structures have been proposed to play a role in modulating these responses. to understand early events related to immune modulation during cestode infection, we have examined the role of intact glycans of antigens from taenia crassiceps in the recruitment of innate cells. soluble antigens from ... | 2005 | 16179033 |