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proceedings: further studies on the effect of taenia crassiceps on rats and mice. 19744419379
taenia crassiceps: absorption of hexoses and partial characterization of na + -dependent glucose absorption by larvae. 19734691924
freeze-etch characterization of the teguments of three metacestodes: echinococcus granulosus, taenia crassiceps, and taenia taeniaeformis.the objective of this study was to characterize the teguments of metacestodes of echinococcus granulosus, taenia crassiceps, and taenia taeniaeformis using the freeze-etch technique. metacestodes of e. granulosus (19 mo old), t. crassiceps (28 days old), and t. taeniaeformis (34 days old) from gerbils, mice and rats, respectively, were fixed for 2 hr in 3% glutaraldehyde and then prepared for freeze-etching and thin sectioning by standard techniques. freeze-etch replicas of the teguments of all ...19836685176
intraocular taenia crassiceps (cestoda). ii. the parasite. 19734717691
mechanisms and specificity of amino acid transport in taenia crassiceps larvae (cestoda). 19734741640
[counterimmunoelectrophoresis in the serologic diagnosis of cysticercosis in cattle].over the years 1983 to 1984 two-hundred and sixty-five bulls were examined during mass occurrence of bovine cysticercosis in one farm; out of this number 67 animals (25.3%) were confirmed autopsically as infected with cysticercosis. counterimmunoelectrophoresis was used to examine the serum of slaughtered animals by help of two antigens--taenia saginata and taenia crassiceps. with t. saginata the sensitivity amounted to 44.8% and the specificity to 86.4%, with t. crassiceps to 28.3% and to 89.9% ...19863083565
the intestinal helminths of the red fox and some other carnivores in southwest germany.in south-west germany between 1975 and 1980, 3,573 red foxes (vulpes vulpes), 84 badgers (meles meles), 47 stone martens (martes foina), and 387 cats (felis catus) were examined for intestinal helminths. in foxes the extent of infections was: taenia crassiceps 24%, t. polyacantha 8%, t. taeniaeformis 0.6%, t. serialis 0.5%, mesocestoides leptothylacus 20%, mesocestoides sp. 0.2%, toxocara canis 32%, toxascaris leonina 3%, uncinaria stenocephala 26%. one to three foxes harboured t. hydatigena, t. ...19827072323
chalone-like inhibition of dna synthesis in helminths.chalone-like inhibitory activity was found in extracts from tissues of taenia crassiceps cysticercus and tissues of adult ascaris suum. the inhibitory effects were partially species nonspecific: the extracts from t. crassiceps larvae inhibit both t. crassiceps and mesocestoides corti larvae dna synthesis. extracts from a. suum, however, inhibit only t. crassiceps. besides them, the inhibitory factor from mouse brain inhibits both t. crassiceps and m. corti dna synthesis. when the inhibitory fact ...19853159635
phosphoglycerides and derivatives in taenia crassiceps examined by 31p nmr spectroscopy.examination of the larval stage of the tapeworm, taenia crassiceps, by 31p nmr spectroscopy revealed the presence of a major phosphoglyceride component. however, using saturation transfer, no exchange between glycerophosphorylcholine and phosphoglyceride or any other nmr-detectable phosphorus metabolites was detected.19883357104
comparison of the in vitro translation capacity of taenia crassiceps metacestode mrna prepared by the phenol and cesium chloride method.total rna of t. crassiceps metacestodes harvested from male and female nmri mice was prepared by both the phenol extraction technique and cesium chloride (cscl) gradient centrifugation. mrna was selected by oligo (dt)-cellulose affinity chromatography and used as the template for the in vitro translation of parasite polypeptides in a cell-free rabbit reticulocyte lysate. the template activity of the mrna obtained after cscl preparation was clearly higher, as shown by the amount of 35s-methionine ...19883413044
transhydrogenase activities in the mitochondria of taenia crassiceps (zeder, 1800) cysticerci.nadph/nad and nadh/nad transhydrogenase activities have been demonstrated in the mitochondrial fraction of taenia crassiceps cysticerci. these activities seem to result from the activities of two different enzyme systems. both transhydrogenase activities exhibited a high heat resistence and they were completely abolished only by the temperatures higher than 100 degrees c. the activity of nadh/nad transhydrogenase was rather high (116 nmol.min-1.mg-1 protein), but it was found to exhibit a low af ...19883417198
tapeworm larvae (taenia crassiceps) in woodchucks. 19725056190
growth and development of larval taenia crassiceps (cestoda). 3. the relationship between larval biomass and the uptake and incorporation of 14 c-leucine. 19725057037
some effects of sub-lethal x-irradiation on reproduction and development in two strains of larval taenia crassiceps. 19715156167
further studies on the hook development of taenia crassiceps (zeder, 1800). 19705488857
chromatographic and antigenic properties of echinococcus granulosus hydatid cyst-derived glycolipids.the neutral and acidic fraction glycolipids of echinococcus granulosus metacestode tissue compartments were isolated, defined by their chromatographic and antigenic properties, and assessed as to their efficacy as antigens in the serodiagnosis of human hepatic cystic and alveolar echinococcosis, and other helminthiases. analyses were accomplished by thin-layer chromatography immunostaining and elisa. the neutral glycolipid fraction's major carbohydrate epitope was the same as or very similar to ...19937533281
[invasive cysticercosis due to taenia crassiceps in an aids patient].a first case is reported from france of a subcutaneous and muscular tissues invasion by proliferative larval forms of taenia crassiceps in an aids-patient with severe immunodeficiency. these larvae usually develop in rodents whereas the adult form of the cestode is commonly found in foxes. authors emphasize the differential diagnosis from other human subcutaneous metacestodoses such as t. solium cysticercosis, cenurosis and hydatidosis. the proliferative aspect of the larvae, the recurrence of l ...19957648312
preparation and sequence analysis of taenia crassiceps metacestode recombinant antigens with potential for specific immunodiagnosis of human cerebral cysticercosis.a taenia crassiceps metacestode cdna expression library in lambda gt 11 was screened with rabbit antisera to metacestodal t. solium and t. saginata crude extract. primary clones (121) were identified, and after rescreening and lysogenization in escherichia coli y 1089, were tested in western blot for reactivity with the same antisera. in addition, analyses were performed with rabbit antisera directed towards t. crassiceps and echinococcus granulosus metacestode crude extract, sera from humans wi ...19947716396
the uptake of 14c-chlorella protein by larval taenia crassiceps (cestoda). 19715550314
abnormalities in the development of taenia crassiceps (zeder, 1800). 19675608353
observations on the structure of oncosphere of taenia crassiceps (zeder, 1800) rudolphi, 1810. 19685724486
studies on the metabolism of larval tapeworms (cyclophyllidea: taenia crassiceps). i. amino acid composition before and after in vitro culture. 19665938095
a taenia crassiceps cdna sequence encoding a putative immunodiagnostic antigen for bovine cysticercosis.a cdna expression library was constructed in lambda gt11 using poly a mrna from the metacestode stage of taenia crassiceps. the library was screened with rabbit antiserum to a previously defined protein fraction from taenia hydatigena immunodiagnostic for bovine cysticercosis and with sera from cattle with experimentally induced cysticercosis. one clone (lambda tca2) containing a 279-bp cdna insert, reacted strongly with both antisera. a second clone (lambda tca5.5) revealed the full-length cdna ...19947870126
the specificity of somatic and metabolic taenia taeniaeformis preparations in murine metacestode infections.excretory-secretory and somatic preparations of taenia taeniaeformis contained shared immunologically-active components although immunoelectrophoresis indicated that the major antigen present in the excretory-secretory preparation was only a minor component of the somatic preparation. both antigens gave similar immunoelectrophoretic reactions with sera from mice infected with either t. taeniaeformis or taenia crassiceps, but there was evidence from the results using the elisa technique that the ...19836195803
taenia crassiceps: temperature, polycations, polyanions, and cysticercal endocytosis.the effect of various temperatures, poly-l-lysine, and poly-l-glutamic acid on endocytosis of smooth micropinocytotic vesicles (pinosomes) in the tegument of the cysticercus of taenia crassiceps has been investigated stereologically. the temperature regimes used were 0, 5, 10, 20, 30, and 40 c. maximum volume, surface density, and number per unit volume were found at 40 c, and minimum surface-to-volume ratio and numbers at 10 c. at 10 c, mean pinosome volume and mean surface area per pinosome we ...19846204882
substrate specificity of fumarate reductase activity of taenia crassiceps mitochondria.the substrate specificity of fumarate reductase activity of taenia crassiceps mitochondria is surprisingly low. possible practical utilization of this phenomenon is discussed.19938106189
taenia crassiceps: basic mechanisms of endocytosis in the cysticercus.the effects of glucose, yeast extract, fetal bovine serum albumin, and ruthenium red on endocytosis of smooth micropinocytotic vesicles (pinosomes) in the tegument of the cysticercus of taenia crassiceps have been investigated stereologically. glucose has been shown to stimulate pinocytosis, whether it was used alone or in combination with yeast extract or bovine serum albumin. yeast extract was a stimulant of endocytosis. bovine serum albumin was the most potent stimulant of all the substances ...19846209161
[hydatidosis immunodiagnosis: determination of taenia crassiceps cysticerws antigens in hydatid and vesicular fluid].the specificity and sensitivity of the enzyme immunoassay (eia), presently used in south america areas where hydatidosis caused by echinococcus granulosus is endemic, was compared to two alternative eia. one of these uses an hydatid antigen of different preparation and the other vesicular fluid of taenia crassiceps cisticerci (vfcc). the effect of previous neutralization in the serum sample of antibodies antinormal ovine or murine sera and antiphosphorylcholine on the diagnostic efficiency of th ...19938284600
scanning electron microscopy of the metacestode of taenia crassiceps. 19836618754
the pathology induced in laboratory rats by metacestodes of taenia crassiceps and mesocestoides corti (cestoda).sprague-dawley rats were infected by intraperitoneal injection with either the cysticerci of taenia crassiceps or the tetrathyridia of mesocestoides corti. both species of metacestodes reproduce asexually in the peritoneal cavity. the effect of t. crassiceps upon the rats was to cause chronic inflammation of the peritoneum, fibrous adhesions between the visceral organs and hyperplasia of the gut mesenteries which became extended into a large sac-like structure. dead parasites surrounded by white ...19836646809
taenia crassiceps: ultrastructural observations on the oncosphere and associated structures.electron microscopic observations were made on unhatched eggs of taenia crassiceps in utero. the outermost envelope consists of a thin, relatively smooth capsule over a highly convoluted outer envelope which contains a highly granulated cytoplasm and numerous mitochondria. the inner envelope, consisting of a thick embryophore and the cytoplasmic component of the embryophore cell, resembles that found in most other taeniids. three epithelial layers separate the oncosphere from the embryophore. wh ...19836683735
subcutaneous taenia crassiceps cysticercosis in a dog.taenia crassiceps larvae, typically found in rodents, were recovered from fluctuant subcutaneous cystic lesions in a dog. differential diagnosis included taenia crassiceps cysticercosis, other metacestode infections, and pseudotumoral skin diseases.19938407486
in vivo cross-reactivity of taenia saginata and taenia crassiceps antigens in bovine cysticercosis.steers sensitized or infected with taenia saginata exhibited similar delayed-type dermal hypersensitivity (dth) responses after intradermal inoculation with t. saginata or t. crassiceps skin test antigens. steers sensitized to t. crassiceps cysticerci exhibited similar dth responses to intradermal inoculation with t. crassiceps, t. saginata whole worm and t. saginata cysticerci antigens. no correlation existed between the dth responses and the number of cysticerci in the carcasses. one sensitize ...19836684352
the nature of antigens common to both the metacestodes of taenia crassiceps and its laboratory host.metacestodes of taenia crassiceps were cultured in laboratory rats and mice, washed in glycine/hydrochloric acid (gly/hcl) buffer ph 2.4 and phosphate buffered saline (pbs) ph 7.2 and a soluble extract of the metacestodes (sem) was prepared from larvae washed in both buffers. the sem and the original washings were examined for host igg and a common host antigen (cha). cha was present in both the sem and washings but igg only in the washings and, in metacestodes from mice only in the gly/hcl wash ...19826820025
taenia crassiceps: cloning and mapping of mitochondrial dna and its application to the phenetic analysis of a new species of taenia from southeast asia. 19958543003
taenia crassiceps: regional variations in ultrastructure and evidence of endocytosis in the cysticercus' tegument.regional variations in the thickness of the tegument, in the morphology of microtriches and mitochondria, in the distribution of dense bodies, smooth micropinocytotic vesicles (smvs), and coated micropinocytotic vesicles (cmvs) have been shown for the cysticercus of taenia crassiceps. the number of smvs and cmvs present in the syncytial layer are in inverse proportion to each other, the former being more numerous in the bladder wall and upper part of the invagination canal and the latter in the ...19836822284
the growth and antigenicity of the metacestodes of taenia crassiceps in several different strains of rats.the metacestodes of taenia crassiceps removed from a stock infection of sprague dawley rats were injected into seven inbred and one outbred strain of rats. the results showed that over-all female rats are more susceptible to the infection than male rats. only one strain of rat, the lewis male rats, was found to be resistant to the infection. there was no significant differences in the volume of metacestodes recovered from the male and female rats. the sizes of the metacestodes, after two passage ...19816974186
cysticercosis: identification and cloning of protective recombinant antigens.we describe the cloning and the evaluation of the protective capacity of 5 recombinant antigens expressed during the cysticercus stage of both taenia crassiceps and taenia solium. a cdna library was constructed in bacteriophage lambda zap using mrna isolated from larvae of t. crassiceps of the orf strain. the recombinant phage library was screened with polyclonal antibodies against 56- and 74-kda protective antigen fractions. this screening identified 13 recombinant clones, 5 of which were also ...19968604092
permeability and membrane transport in the larva of taenia crassiceps. 19734786832
taenia crassiceps: antigenic comparison of two larval strains. 19714997335
recovery of taenia crassiceps cysticerci (ers toi derived strain) from sub-zero temperature. 19827096968
taenia crassiceps: fate of cysticerci following ingestion by the mouse. 19827151949
[2 types of secretory neurons in taenia crassiceps (cestoda: taeniidae) larvae]. 19817332672
[helminths of the common fox (vulpes vulpes l.) from the massif central (france) (author's transl)].sixty nine wild foxes originating from auvergne for the period january 1977 to january 1978 have shown intestinal parasites in the proportion of 76,8 per cent. 59,4 per cent are invaded with cestodes; 72,4 per cent with nematodes and none with trematodes. the list of the identified species is following: 1. cestodes. a) adults and fertile forms of: mesocestoides litteratus in 27,5 per cent of the hosts; taenia crassiceps in 15,9 p.c.; t. polyacantha in 27,5 p.c.; echinococcus multilocularis in 7, ...19807458165
inhibition of sexual behavior in male mice infected with taenia crassiceps cysticerci.prominent estrogenization and deandrogenization ensue in male mice as a consequence of experimental intraperitoneal infection with taenia crassiceps cysticerci. the impact of these endocrine changes upon sexual behavior was explored in a group of infected balb/c male mice at weekly intervals for 15 wk and compared with the behavior of otherwise paired, nonparasitized male mice. mounting, intromission, and ejaculation responses markedly declined as infection progressed. six weeks after infection, ...19968885872
depressed t-cell proliferation associated with susceptibility to experimental taenia crassiceps infection.peritoneal infection with taenia crassiceps cysticerci of naturally resistant (c57bl/10j and c57bl/6j) and susceptible (balb/cann) mice induces a cellular immune depression. t-cell proliferation in response to concanavalin a (cona) or anti-cd3 was significantly depressed in infected mice of all strains tested. however, in resistant mice, the diminished response to cona was transient and animals recovered normal responsiveness at day 40, whereas susceptible mice remained suppressed throughout the ...19957768609
sex hormone changes induced by the parasite lead to feminization of the male host in murine taenia crassiceps cysticercosis.female mice are more susceptible to taenia crassiceps (tc) infection than males. however, after a month parasite load increases massively in both genders reaching thousands of parasites per host. the possibility of hormonal changes in the infected mice was envisaged. sex hormones levels were assayed after different periods of infection, the parasites present in the peritoneal cavity were collected and gonads, uterus and seminal vesicles were weighed. in male mice, serum estradiol increased to le ...19957779761
immunization against taenia crassiceps cysticercosis: identification of the most promising antigens in the induction of protective immunity.cross immunity between taenia solium and taenia crassiceps parasites points to t. crassiceps cysticercosis as a convenient model to test promising antigens aimed at the development of a vaccine against t. solium cysticercosis. since total antigens from t. crassiceps metacestodes induce significant levels of protection in pigs against t. solium cysticercosis, we initiated this work to identify the most interesting antigens involved in protection. twelve different antigen fractions isolated from t ...19947799166
protein uptake by cysticerci of taenia crassiceps.the internalization of host macromolecules to the vesicular fluid of t. crassiceps cysticerci was studied in vitro. uptake of purified class g immunoglobulin was not significantly affected by the specificity of its antigen-recognition site and bovine serum albumin was internalized at a similar rate. internalization was inhibited at low temperature, being optimal at 37 degrees c and saturation was accomplished only at a protein concentration in the culture medium over 12 mg/ml which is close to t ...19947803983
[the helminth fauna of the red fox (vulpes vulpes linne 1758) in the administrative district of karlsruhe. 1. cestodes].between february 1989 and january 1990 a total of 801 red foxes were examined for the presence of cestodes. echinococcus multilocularis could be noted with an infection rate of 11.6% in the small intestine of the red fox. the following other cestodes were found (in order of frequency) taenia crassiceps (19.9%), mesocestoides spp. (16.6%), taenia polyacantha (7.0%), hydatigena taeniaeformis (0.7%), dipylidium caninum (0.5%) and diphyllobothrium latum (0.5%). infections with echinococcus multilocu ...19947924977
a role for 17-beta-estradiol in immunoendocrine regulation of murine cysticercosis (taenia crassiceps).in experimental murine cysticercosis caused by taenia crassiceps, parasite reproduction is favored by thymectomy or by orchidectomy, and restricted by ovariectomy. hormonal reconstitution experiments showed that 17-beta-estradiol increases parasite numbers whereas 5-alpha-dihydrotestosterone was ineffective. parasite numbers decreased with increments in cellular immunity but were insensitive to antibody levels. a possible immunoendocrinological interaction involving estrogen as a depressor of ce ...19948064524
infection course of the strobilar stage of taenia crassiceps in golden hamsters, with reference to host responses.the time course of intestinal infection of golden hamsters (mesocricetus auratus) with taenia crassiceps was monitored every 2 days up to day 26 postinfection (p.i.). the isolate used was originally obtained from clethrionomys rutilus on st. lawrence island, bering sea (usa), and shows a high level of enteral establishment without parenteral infection. following oral administration of various numbers (3, 15, 30, or 60) of metacestodes, proportional numbers of cestodes attached their scolices to ...19948202462
establishment and survival of the strobilar stage of taenia crassiceps in hamsters, gerbils, and mice, with reference to different helminth isolates.following the oral administration of metacestodes of two isolates of taenia crassiceps, the enteral establishment and survival of the strobilar stage were examined in golden hamsters (mesocricetus auratus), mongolian gerbils (meriones unguiculatus) and laboratory mice. the origin of the isolates was microtus montebelli caught in japan in 1985 or clethrionomys rutilus captured on st. lawrence island, bering sea, in 1988 (abbreviated as jpn and sli isolates), respectively. the enteral establishmen ...19938295896
[contributions to the parasite fauna of local hosts. 10. on the endoparasitic fauna of felis silvestris].the investigations based on examination of the internal organs of 25 wild cats of the harz population died from different causes in the period from 1982 to 1989. 22 animals (= 88%) were positive for parasites. the following species were found: hydatigera taeniaeformis, taenia crassiceps, t. martis larv. (spurious parasite), mesocestoides litteratus, capillaria plica, capillaria sp. (aerophila?), toxocara mystax, cystoisopora felis. toxocara mystax and hydatigera taeniaeformis were the most often ...19938334454
glycosphingolipids of the invertebrata as exemplified by a cestode platyhelminth, taenia crassiceps, and a dipteran insect, calliphora vicina. 19938379457
an igg (fc gamma)-binding protein of taenia crassiceps (cestoda) exhibits sequence homology and antigenic similarity with schistosome paramyosin.previous studies from this laboratory have suggested the presence of antibody-binding molecules on the surface of taenia crassiceps metacestodes. we now report the purification of a t. crassiceps fc gamma-binding protein which has an equivalent molecular size (96 kda), is antigenically similar and exhibits significant amino acid sequence homology to schistosome paramyosin. the similarities in molecular weight of the t. crassiceps protein, schistosoma mansoni paramyosin and antigen b of t. solium ...19938488065
depressed immunity to a salmonella typhimurium vaccine in mice experimentally parasitized by taenia crassiceps.to assess the immunological status of mice parasitized with taenia crassiceps metacestodes, 6-month old female balb/c mice experimentally parasitized with t. crassiceps and immunized with salmonella typhimurium antigens were infected with s. typhimurium virulent bacilli (1.6 x ld50). both t. crassiceps-parasitized and immunized and parasitized mice showed a very high susceptibility to infection (**p < 0.01) with higher bacteremia than control and immunized-control animals and produced a reduced ...19989561706
thymus-related cellular immune mechanisms in sex-associated resistance to experimental murine cysticercosis (taenia crassiceps).the role of sex, thymus, and cellular immune mechanisms in mouse resistance to experimental cysticercosis with taenia crassiceps was studied in male and female susceptible mice treated with cyclophosphamide, as well as in mice neonatally thymectomized and passively transferred with t-enriched lymphoid cells. high doses of cyclophosphamide increased delayed hypersensitivity and resistance of mice of both sexes without affecting antibody production. neonatal thymectomy diminished resistance in bot ...19938501595
antigens from taenia crassiceps cysticerci used in complement fixation, enzyme-linked immunosorbent assay, and western blot (immunoblot) for diagnosis of neurocysticercosis.antigens from taenia solium cysticerci for the immunodiagnosis of cysticercosis are scarce and difficult to obtain. we studied the reliability of antigens from taenia crassiceps cysticerci as a substitute for those from t. solium in three diagnostic tests: complement fixation, enzyme-linked immunosorbent assay, and western blot (immunoblot). precision rates of the three tests of 93, 99, and 96%, respectively, were found. cysticerci from t. crassiceps can be easily obtained in large quantities an ...19958586728
genetic control of susceptibility to taenia crassiceps cysticercosis.we previously reported that genes within the major histocompatibility complex influence the intensity of taenia crassiceps murine cysticercosis. this genetic control, readily apparent in mice of balb background, was further studied in h-2 congenic and recombinant b10 mice as well as in balb/c substrains differing in expression of qa-2 antigens. similarly low parasite numbers were found in all b10-derived strains infected, regardless of h-2 haplotype, indicating that the effect of h-2 genes in co ...19968587794
infectivity of hymenolepis diminuta for the jird, meriones unguiculatus, and utility of this model for anthelmintic studies.the jird (meriones unguiculatus) has been shown to be a useful model host for the cestodes taenia crassiceps and echinococcus multilocularis. this report outlines a novel model in which hydrocortisone-treated jirds (0.02% in the feed) are infected with another cestode, hymenolepis diminuta. jirds were inoculated with 5 freshly harvested cysticercoids of h. diminuta prior to (day 0, -1, or -5) or after (day 1 or 5) switching to medicated feed; in some cases, jirds were never medicated. on days 7, ...19968636859
immunization of pigs against taenia solium cysticercosis: factors related to effective protection.fifty-six (56) pigs were immunized against taenia solium cysticercosis with antigens from taenia crassiceps metacestodes, in a variety of protocols, and then challenged orally with taenia solium proglottids or eggs. results of immunization (expressed as individual parasite loads) ranged from significant reduction of parasite loads (host protection) to clear increase (parasite facilitation) in apparent relation to the immunogen dose, adjuvant employed and genetic background of the pigs. in all tr ...19958644459
taenia crassiceps: serum and surface immunoglobulins in metacestode infections of mice. 19817227489
[contribution to the knowledge of taenia crassiceps (zeder, 1800) rudolphi, 1810 (cestoda, taeniidae) (author's transl)].the occurrence of taenia crassiceps in naturally infected dogs is mentioned, and a brief description of the gravid proglottids is given. oral infection of several rodent species with eggs showed that the field vole (microtus arvalis) is the most susceptible intermediate host for this tapeworm. the cysticercus often develops in the brain of the rodent and causes disturbances of coordinated movements. two foxes (vulpes vulpes) were infected with cysticerci of the strains cola and giks. these strai ...19817314812
[rostellar organ or taenia crassiceps (cestoda: taeniidae) larvae].there are glandular elements of syntitial structure in parenchyma of scolex base of t. crassiceps larvae. they are located behind the suckers at the invaginated state of scolex. perikaryons of gland contain dense rodshaped, spheric granules and great accumulations of electron-dense substance in vacuoles. the same types of secretary product are found in the rostellar tegument. the glandular elements are mostly developed in the period of the formation of rostellar hooks, but they are well expresse ...19817322618
intestinal helminths of the red fox (vulpes vulpes l.) in galicia (northwest spain).the intestinal helminth fauna of 201 specimens of vulpes vulpes, captured in galicia (northwest spain), was investigated. eight species were found: toxocara canis (23% of host specimens), toxocara cati (0.5%), toxascaris leonina (1%), uncinaria stenocephala (28%), seuratascaris numidica (0.5%), taenia crassiceps (23%), mesocestoides litteratus (2.5%) and dipylidium caninum (0.5%). we discuss possible relationships between human population density and the prevalence of infection of fox by intesti ...19958834508
experimental intravitreous cysticercosis.cysticercosis is one of the parasitic diseases that most frequently affects the eye. the most common and severe manifestations of ocular infection are secondary to posterior segment involvement, which often leads to blindness and atrophy of the eye. the pathogenesis of ocular injury in this disease is poorly understood. the authors have developed an experimental animal model for intravitreous cysticercosis using new zealand rabbits and taenia crassiceps cysticerci.19968858358
immunodiagnosis of swine cysticercosis by indirect elisa employing a heterologous antigen from taenia crassiceps metacestode.c. larralde et al. (1990, aoff. pathol. lab. med., 114:926-928) demonstrated that heterologous antigen from the laboratory-adapted murine taenia crassiceps metacestode may substitute those from taenia solium in the immunodiagnosis of human cysticercosis by the indirect enzyme-linked immunosorbent assay (ie). this antigen is easily obtained at a laboratory level and solves the problem of t. solium cysticerci collection from naturally or experimentally infected swine. in this study an ie employing ...19968893480
immunological responses in the mouse host to a cloned antigen of taenia crassiceps.adult female swiss-webster mice were immunized either intraperitoneally (ip) or subcutaneously (sq) with cyst fluid or a genetically engineered fusion protein, taenia crassiceps antigen 2-maltose binding protein (tca2-mbp) from taenia crassiceps metacestodes, or with live, non-budding cysts sq, and then challenged ip with t. crassiceps metacestodes and necropsied 9 weeks later. numbers of peripheral blood eosinophils were increased after ip immunization, but were not increased after sq immunizat ...19968983150
in vitro culture of taenia crassiceps larval cells and cyst regeneration after injection into mice.taenia crassiceps cysticerci were disrupted through trypsinization to isolate cells which can be maintained in culture for up to 15 days. when injected intraperitoneally into susceptible balb/cann mice, complete cysticerci were recovered in a number that is proportional to the quantity of injected cells. thus, cysticerci contain cells which can reconstitute complete cysts, suggesting that individual cells play a role, independent to budding, during asexual multiplication of t. crassiceps cystice ...19979105294
characterization of a cysteine proteinase from taenia crassiceps cysts.human neurocysticercosis, due to infestation of the central nervous system with taenia cysts, is a common cause of neurologic disease in endemic areas and is being increasingly recognized in the united states. previous studies have suggested that taenia cysts bind host igg via fc-like receptors and that bound igg is degraded by the parasite, perhaps as a source of nutrients or a means of immune evasion. we now demonstrate that igg degradation is thiol dependent and is inhibited by the cysteine p ...19979106197
granuloma cytokines in murine cysticercosis.neurocysticercosis, caused by taenia solium, is one of the most common causes of seizures worldwide. the symptoms result from granulomatous inflammation associated with dying cyst forms of the parasite. although the invasive larvae can be killed by immune serum plus complement, immunity to the cyst stage depends on a cellular response. this dichotomous immune response is reminiscent of the extremes of the immune response associated with t helper 1 (th1) and th2 cytokine profiles. to characterize ...19979199468
gamma delta t cells do not play a major role in controlling infection in experimental cysticercosis.protective immunity against larval taenia crassiceps has been shown to rely on t cells; however, the roles of the specific subsets of t cells during infection are not known. to investigate a possible role for gamma delta t cells, this study investigated larval infection in delta-chain knock-out c57bl/6 (deltako) and wild-type c57bl/6 mice. it was found that deltako mice and c57bl/6 mice were equally susceptible to infection suggesting gamma delta t cells do not play a major role in protective im ...199910581620
[helminth fauna of the red fox (vulpes vulpes linne 1758) in south sachsen-anhalt--1: cestodes].between january 1993 and november 1994 a total of 1300 red foxes from the administrative districts halle and dessau were examined for the presence of echinococcus multilocularis and other cestodes. echinococcus multilocularis-infections were detected in only 4 of 1300 (0.3%) foxes. furthermore the following cestodes were found: mesocestoides spp. (54.1%), taenia crassiceps (17.7%), taenia polyacantha (11.9%), hydatigera taeniaeformis (0.2%), taenia serialis (0.15%), taenia pisiformis (0.15%), no ...19979445786
specific taenia crassiceps and taenia solium antigenic peptides for neurocysticercosis immunodiagnosis using serum samples.neurocysticercosis (nc), i.e., the presence of the larval form of taenia solium in tissues, is the most frequent and severe infection involving the central nervous system. paired serum and cerebrospinal fluid (csf) samples from patients with nc, csf and serum samples from a control group, and serum samples from patients with other parasitoses were studied by enzyme-linked immunosorbent assay (elisa) and by immunoblotting with taenia crassiceps vesicular fluid antigen (tcra) and taenia solium tot ...200010618078
fatal cysticercosis by taenia crassiceps (cyclophyllidea: taeniidae) in a presumed immunocompromised canine host.cysticercosis in a canine host (canis familiaris) attributable to the taeniid cestode taenia crassiceps is reported for the first time in north america. numerous parent and daughter cysticerci occurred in a massive intrapleural and intraperitoneal infection in an apparently immunocompromised host. the largest cysticerci were ovoid to elongate, 5-9 mm in maximum length, and armed with 32-34 rostellar hooks in 2 rows; small hooks measured 114-143 microm long (x = 124+/-8.2 microm), and large hooks ...199910647054
increased resistance to taenia crassiceps murine cysticercosis in qa-2 transgenic mice.we previously reported important differences in resistance to taenia crassiceps murine cysticercosis between balb/c substrains. it was suggested that resistance might correlate with expression of the nonclassic class i major histocompatibility complex (mhc) qa-2 antigen; balb/cann is qa-2 negative and highly susceptible to t. crassiceps, whereas balb/cj expresses qa-2 and is highly resistant. in this study, we investigated the role of qa-2 in mediating resistance to cysticercosis by linkage anal ...19989453638
shift from an early protective th1-type immune response to a late permissive th2-type response in murine cysticercosis (taenia crassiceps).in early stages of experimental murine cysticercosis caused by taenia crassiceps, there is a clear but transient th1-type immune response (characterized by high levels of interleukin [il]-2, interferon-gamma, concanavalin a, and antigen specific response, delayed-type hypersensitivity, and immunoglobulin [ig]g2a antibodies) that associates with a low rate of parasite reproduction. as time of infection progresses an energic and more permanent th2-type response follows (characterized by high level ...19989488341
taenia crassiceps invasive cysticercosis: a new human pathogen in acquired immunodeficiency syndrome?a fluctuant, painful, subcutaneous, and intermuscular tumor developed in a 38-year-old man with severe acquired immunodeficiency syndrome (aids) in which immunodeficiency was severe. surgery revealed lesions that formed a multilocular pouch embedded in deep tissues in the forearm filled with tapiocalike material containing a viscous fluid, granules, and cysticercilike small vesicles. pathologic and parasitologic evaluation showed cysticerci embedded in a fibrocollagen reaction with inflammatory ...19989537478
two taenia species found in japan, with new distribution record of taenia polyacantha leuckart, 1856 (cestoda: taeniidae).in an epidemiological survey for echinococcus multilocularis in rodents and insectivores from the northernmost part of the central mainland of japan (honshu), two taeniid species, taenia crassiceps and taenia polyacantha, were found in microtus montebelli and apodemus argenteus, respectively. the latter is the first record of distribution in japan, and the former is the second after its first recovery from the central part of japan. although we have found neither larval nor strobilar stage of e. ...200010725694
a method for the isolation of tegument syncytium mitochondria from taenia crassiceps cysticerci and partial characterization of their aerobic metabolism.heterogeneous populations of mitochondria have been described in helminths. mitochondria from different tissues have been isolated in adult organisms. however, in larvae, due to their small size, isolation from tissues has not been feasible. a method for the isolation of tegumental mitochondria from the larval stage of taenia crassiceps is described. after solubilization of the plasma membrane with saponin, tegumental mitochondria were purified by a simple and rapid protocol of differential cent ...19989645840
demonstration of taenia crassiceps cysteine proteinase activity in tegumentary lysosome-like vesicles.larval stages of taenia species survive for prolonged periods in the tissues of their intermediate hosts. other groups have demonstrated that host immunoglobulins are taken up by the cysticerci by adsorptive endocytosis, degraded, and the amino acids incorporated into parasite proteins. we have shown that a 43-kda cysteine proteinase is the major parasite enzyme that degrades immunoglobulin in vitro. to localize this enzyme in situ, taenia crassiceps cysticerci were incubated with the peptide su ...19989645848
taenia crassiceps cysticercosis: humoral immune response and protection elicited by dna immunization.the purpose of this study was to evaluate dna vaccination in cysticercosis prevention by using a taenia crassiceps cdna of a recombinant antigen (ketc7) that has been reported as protective against murine cysticercosis. the ketc7 cdna was cloned into the pcdna3 plasmid alone or with the betaglycan signal peptide sequence (ptc-7 and ptc-sp7, respectively). positive expression of the ptc-sp7 product was confirmed by transfection of c33 cells and immunofluorescence using sera of mice infected with ...19989645849
metabolism of [3-(13)c]-pyruvate by cysticerci of taenia crassiceps.carbon-13 decoupled 1h spin-echo nuclear magnetic resonance (nmr) spectra, with and without 13c population inversion, of extracts of taenia crassiceps cysticerci incubated in media containing [3-(13c)]-pyruvate showed 13c enrichment in alanine, lactate, acetate, succinate, and citrate. labeling in the latter three metabolites provides substantial evidence that the malic enzyme reaction in this cestode also functions in the direction opposite to that in which it is normally portrayed. the direct ...19989660145
a high degree of cross reactivity between the antigens of taenia solium and taenia crassiceps, potentially useful for the diagnosis of human neurocysticercosis (ncc) 19989660447
protection against murine cysticercosis using cdna expression library immunization.genetic or dna-based immunization, including genomic immunization, has shown to be a viable alternative approach to induce protective immunity against a number of pathogens in several disease models. here we describe a new method, cdna expression library immunization (cdeli), based on the use of a large number of cdna clones. this immunization strategy was tested in experimental murine taenia crassiceps cysticercosis model. a partial cdna expression library of 2 x 10(4) members was constructed i ...19989698110
mice infected with the larvae of taenia crassiceps exhibit a th2-like immune response with concomitant anergy and downregulation of th1-associated phenomena.infection of intermediate hosts with eggs of taeniid parasites results in a larval infestation known as cysticercosis. a number of studies have indicated that cysticercosis is associated with immunosuppression, although little is known about the mechanisms involved. in the present study, mice infected with the larvae of taenia crassiceps were found to exhibit a pronounced energy, which preferentially affected t-cells located anatomically close to the parasite. this anergy was linked to late even ...19968684830
immunodominant synthetic peptides of taenia crassiceps in murine and human cysticercosis.the screening of a cdna library of taenia crassiceps revealed a clone designated ketc7 that induced high levels of protection against murine cysticercosis in previous experiments. the molecular structure of the deduced 100-amino acid sequence of the corresponding proline-rich polypeptide was studied to detect potentially immunologically active epitopes. several candidate epitopes were identified, three of which were synthesized by solid-phase peptide synthesis and used as antigens in enzyme-link ...19968739315
metabolic profile of the liver of mice infected with cysticerci of taenia crassiceps. 19968801565
immunofluorescent localization of intermediate filaments (ifs) in helminths using anti-mammalian ifs monoclonal antibody.intermediate filaments (ifs) make up the cytoskeleton of most eukaryotic cells. in vertebrates, a number of if proteins have been identified, showing distributions unique to tissue or cell type. information on helminth ifs is limited to some nematode species. to observe immunofluorescent localization of ifs in helminth tissues, we selected a murine hybridoma clone producing igm antibody to multiple types of mammalian if proteins and examined cross-reactivity to helminth proteins. the selected mo ...200010958445
helminths of foxes and other wild carnivores from rural areas in greece.twenty species of helminth parasites were identified from fox, wolf, jackal and wild cat material collected in greece. of the 314 foxes (vulpes vulpes) examined, 18 helminth species were recovered comprising one trematode, eight cestodes, seven nematodes and two acanthocephalans, with the cestode species mesocestoides sp. (73.2%), joyeuxiella echinorhynchoides (24.5%) and the nematode species uncinaria stenocephala (43.9%), and toxara canis (28.6%) being the most prevalent. five cestode and thre ...19979705680
th1-type cytokines improve resistance to murine cysticercosis caused by taenia crassiceps.resistance and susceptibility to different parasitic diseases have been associated with the predominance of th1- or th2-type immune responses. in experimental murine cysticercosis a th1 response seems to be involved in resistance, whereas th2 activity is associated with heavy parasite intensities. to test this notion the roles of th1- and th2-type cytokines in infected mice were studied after treatment with anticytokine monoclonal antibodies or with recombinant murine cytokines during early stag ...19999934963
mitochondrial genetic code in cestodes.the flatworm mitochondrial genetic code, which has been used for all species of the platyhelminthes, is mainly characterized by aua codon for isoleucine, aaa codon for asparagine and uaa codon for tyrosine. in eight species of cestodes (echinococcus multilocularis, echinococcus granlosus, taenia solium taenia saginata, taenia hydatigena, taenia crassiceps, hymenolepis nama and mesocestoides corti), the cytochrome c oxidase subunit i (coi) genes were partially sequenced to verify this genetic cod ...200011163447
towards a taenia solium cysticercosis vaccine: an epitope shared by taenia crassiceps and taenia solium protects mice against experimental cysticercosis.the taenia crassiceps recombinant antigen ketc7 has been shown to be effective as a vaccine against experimental murine cysticercosis, a laboratory model used to test potentially promising molecules against porcine taenia solium cysticercosis. based on the deduced amino acid sequence of this proline-rich polypeptide, three fragments, gk-1, gk-2, and gk-3, were chemically synthesized in linear form. of the three peptides, only gk-1 induced sterile protection against t. crassiceps cysticercosis in ...199910225916
modified expression of steroid 5 alpha-reductase as well as aromatase, but not cholesterol side-chain cleavage enzyme, in the reproductive system of male mice during (taenia crassiceps) cysticercosis.infection with taenia crassiceps cysticerci in male mice produces an increase in serum estradiol levels, whereas serum testosterone is abolished. concomitantly, complete atrophy of the reproductive tract of infected male mice is observed. the present study was under-taken to determine the expression pattern of three key steroidogenic enzymes in the reproductive tissues of normal and infected male mice. in infected mice, serum estradiol levels were increased 97 times as compared with control mice ...199910227057
susceptibility to trypanosoma cruzi is modified by a previous non-related infection.helminth infections are frequently massive, chronic and strong inductors of th2-type cytokines. this implies that infection by such parasites could alter the susceptibility to subsequent infections by other pathogens, particularly intracellular parasites. we therefore explored whether a persistent infection, caused by taenia crassiceps cysticerci, in balb/c mice could affect susceptibility to a later infection by trypanosoma cruzi. we found that the presence of the cysticerci indeed modified the ...199910320615
the systemic immune response of balb/c mice infected with larval taenia crassiceps is a mixed th1/th2-type response.the subsets of lymphocytes and cytokines regulating the site-specific immune response in experimental cysticercosis (taenia crassiceps) are not known. this study investigated the cells present at the site of infection (pecs) using flow cytometry and measured the cytokines produced by these cells through 50 days of infection. the results showed an expansion of b220+cd5+, b220+cd5-, alpha beta tcr+cd4+ and cd8+ cells coincident with a transient increase in il-10 production. after the initial incre ...199910406041
phage-displayed t-cell epitope grafted into immunoglobulin heavy-chain complementarity-determining regions: an effective vaccine design tested in murine cysticercosis.a new type of immunogenic molecule was engineered by replacing all three complementarity-determining-region (cdr) loops of the human immunoglobulin (ig) heavy-chain variable (v(h)) domain with the taenia crassiceps epitope pt1 (pppvdylyqt) and by displaying this construct on the surfaces of m13 bacteriophage. when balb/c mice were immunized with such phage particles (pigphage), a strong protection against challenge infection in very susceptible female hosts was obtained. when specifically stimul ...199910456929
dna pulsed macrophage-mediated cdna expression library immunization in vaccine development.cdna expression library immunization (cdeli), based on the use of a large number of pathogen's cdna clones, has been previously used in our laboratory in an experimental model of murine taenia crassiceps cysticercosis. in this study we show that ex vivo immunization of mice with macrophages pulsed in vitro with plasmid dna containing cdna expression library (2x10(4) clones) leads to significant reduction of parasite load. additionally, pathogen-specific proliferation of splenocytes from immunize ...199910519926
tissue damage in the male murine reproductive system during experimental taenia crassiceps cysticercosis.chronic infection with taenia crassiceps cysticerci in male mice increases the level of estradiol in serum, whereas it reduces that of testosterone. in addition, male mice lose their typical male reproductive behavior. the effects of cysticerci infection on the histomorphology of male reproductive tissues are unknown. the present study was undertaken to determine the histological changes in testes, seminal vesicles, and prostate of male mice infected with t. crassiceps cysticerci. at 16 wk of in ...199910577725
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