Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| east coast fever: the infectivity for cattle of infective particles of theileria parva harvested in various substrates. | 1973 | 4200007 | |
| cryopreservation of infective particles of theileria parva. | 1973 | 4200405 | |
| letter: infection and transformation of bovine lymphoid cells in vitro by infective particles of theileria parva. | 1973 | 4200607 | |
| studies on colostral antibodies to theileria parva using the indirect fluorescent antibody test. | 1973 | 4200673 | |
| observations on the cross-immunity between theileria lawrencei (serengeti) and theileria parva (muguga) in cattle. | 1973 | 4202666 | |
| growth of theileria parva-infected bovine lymphoid cells in whole-body irradiated mice. | 1974 | 4206061 | |
| east coast fever: field challenge of cattle immunised against theileria parva (muguga). | 1972 | 4210445 | |
| east coast fever: 60co-irradiation of theileria parva in its tick vector, rhipicephalus appendiculatus. | 1974 | 4213045 | |
| east coast fever: quantitative studies of theileria parva in cattle. | 1974 | 4213046 | |
| the specific immunoglobulin response in cattle immunized with isolated theileria parva antigens. | 1974 | 4213482 | |
| the specific immunoglobulin response in cattle immunized with theileria parva (muguga) stabilate. | 1974 | 4213483 | |
| theileria parva: variation in the infection rate of the vector tick, rhipicephalus appendiculatus. | 1974 | 4213496 | |
| east coast fever: titration in cattle of suspensions of theileria parva derived from ticks. | 1974 | 4214585 | |
| immunochemical studies on east coast fever. i. partial segregation and characterization of the theileria parva schizont antigen. | 1974 | 4214909 | |
| immunochemical studies on east coast fever. ii. partial segregation and characterization of the theileria parva piroplasm antigen. | 1974 | 4214910 | |
| immunochemical studies on east coast fever. iii. development of an indirect hemagglutination assay using theileria parva piroplasm antigen. | 1974 | 4214911 | |
| theileria parva: comparative infectivity of a ground tick stabilate and a classical 10-tick challenge. | 1973 | 4215109 | |
| duration of serological response to the indirect fluorescent antibody test of cattle recovered from theileria parva infection. | 1973 | 4217920 | |
| response of whole-body irradiated mice to inoculation of theileria parva-infected bovine lymphoid cells. | 1974 | 4218245 | |
| transformation of reticulum cells to lymphoblasts in cultures of bovine spleen infected with theileria parva. | 1971 | 4323780 | |
| cell fusion, using sendai virus, to effect inter-species transfer of a cell-associated parasite (theileria parva). | 1974 | 4369833 | |
| growth of theileria parva-infected bovine lymphoid cells in irradiated mice. | 1972 | 4502803 | |
| the indirect fluorescent antibody test for experimental east coast fever (theileria parva infection of cattle). evaluatin of dried blood samples as a source of antibody. | 1972 | 4556468 | |
| a capillary-tube agglutination test for the detection and titration of theileria parva and theileria mutans antibodies in bovine serum. | 1972 | 4564332 | |
| the indirect fluorescent antibody test for experimental east coast fever (theileria parva infection of cattle). evaluation of a cell culture schizont antigen. | 1972 | 4564334 | |
| some experiments on the transmission of theileria mutans (theiler, 1906) and theileria parva (theiler, 1904) by the ticks amblyomma variegatum (fabricius, 1794) and rhipicephalus appendiculatus neumann, 1901, in uganda. | 1974 | 4607395 | |
| uptake of 3 h-thymidine during artificial feeding of rhipicephalus appendiculatus ticks infected with theileria parva. | 1972 | 4622849 | |
| some effects of irradiation on theileria parva in the salivary glands of the tick rhipicephalus appendiculatus. | 1972 | 4626453 | |
| the duration of emission of infective particles of theileria parva by infected ticks fed artificially. | 1972 | 4627336 | |
| the indirect fluorescent antibody test for experimental east coast fever (theileria parva infection of cattle). detection of antibodies to schizonts in dried blood samples. | 1972 | 4628843 | |
| attempts to grow tissue culture cells, infected with theileria parva, in laboratory animals. | 1972 | 4630814 | |
| establishment of theileria parva-infected lymphoblastoid cell lines using homologous feeder layers. | 1974 | 4856520 | |
| serological response in cattle to east coast fever (theileria parva infection) as measured by the indirect fluorescent antibody test. | 1969 | 4902152 | |
| application of the indirect fluorescent antibody test in experimental east coast fever (theileria parva infection of cattle). | 1971 | 4934252 | |
| the transmission of theileria parva (theiler, 1904) by rhipicephalus carnivoralis walker, 1965. | 1966 | 4957988 | |
| a preliminary electron microscopic study of east coast fever (theileria parva infection). | 1966 | 4959286 | |
| transition of developmental stages of theileria parva in vitro at high temperature. | 1966 | 4960955 | |
| [fine structure of merozoites of theileria parva]. | 1967 | 4971422 | |
| [electronmicroscopical studies of the multiplication of theileria parva in cattle]. | 1967 | 4971423 | |
| theileria parva: kinetics of replication. | 1969 | 4975359 | |
| the development of theileria parva in the salivary glands of the tick, rhipicephalus appendiculatus. | 1968 | 4984381 | |
| the use of parasympathomimetic drugs to stimulate salivation in the tick rhipicephalus appendiculatus, and the transmission of theileria parva using saliva obtained by this method from infected ticks. | 1969 | 4986780 | |
| theileria parva: comparative infection rates of adult and nymphal rhipicephalus appendiculatus. | 1971 | 4995423 | |
| the fine structure of tick stages of theileria parva. | 1971 | 5002523 | |
| duration of immunity to east coast fever (theileria parva infection of cattle). | 1972 | 5039345 | |
| ifection of rhipicephalus appendiculatus ticks with theileria parva, using a rabbit's head perfusion technique. | 1970 | 5498934 | |
| infection of the tick rhipicephalus appendiculatus with theileria parva using an artificial feeding technique. | 1970 | 5499333 | |
| preliminary experiments with antigens of theileria parva (theiler, 1904). | 1967 | 5624605 | |
| a mild form of east coast fever (theileria parva infection) becoming virulent on passage through cattle. | 1968 | 5652740 | |
| theileria parva: possible serological test for east coast fever. | 1968 | 5726858 | |
| attempts to adapt theileria parva to laboratory animals. | 1963 | 5896628 | |
| observations on the resistance of jersey-nganda calves to east coast fever (theileria parva). | 1963 | 5896629 | |
| organ culture of rhipicephalus appendiculatus with maturation of theileria parva in tick salivary glands in vitro. | a technique is described for the organ culture of rhipicephalus appendiculatus ticks. whole, unfed adult ticks with the dorsal integument removed, known as backless tick explants, were cultured in enriched leibovitz' l-15 medium in which they remained active for at least 32 days at 28 degrees c and 9 days at 36 degrees c. development of theileria parva, as demonstrated by methyl green-pyronin staining, occurred in the salivary glands of infected backless tick explants held for 8 days at 28 degre ... | 1980 | 6110322 |
| monitoring theileria parva infection in adult rhipicephalus appendiculatus ticks. | a rapid method is described for preparing and staining salivary glands of rhipicephalus appendiculatus ticks infected with theileria parva. the technique, involving the use of a modified methyl green pyronin stained minimizes the risk of losing material and allows examination of stained glands within minutes of preparation. the technique was applied in a series of studies in which ticks were either infected with t. parva under different conditions, or maturation of parasites in adult ticks was s ... | 1981 | 6163125 |
| assessment of theileria infections in rhipicephalus appendiculatus ticks collected from the field. | collections of adult rhipicephalus appendiculatus ticks were made from bait cattle and vegetation at two field sites in areas of kenya in which east coast fever caused by theileria parva is endemic. these ticks, together with two experimentally infected batches of ticks, were examined for infection with theileria by four methods. whole salivary glands were stained with methyl green pyronin or feulgen's stain. whole ticks were ground in medium, the suspensions were filtered and centrifuged and th ... | 1981 | 6166132 |
| the incorporation of radio-labelled nucleic acid precursors by theileria parva in bovine blood and salivary glands of rhipicephalus appendiculatus ticks. | 1981 | 6173342 | |
| theileria parva: cell culture analysis of clones of macroschizont-infected bovine lymphoblastoid cells. | 1982 | 6182017 | |
| immune responses of cattle to theileria parva (east coast fever): specificity of cytotoxic cells generated in vivo and in vitro. | examination of the specificity of cytotoxicity generated in vitro and in vivo against infected bovine lymphoblasts revealed that cytotoxic t lymphocytes (ctl) obtained from cattle immune to theileria parva recognized parasite-induced alterations associated with major histocompatibility complex (mhc) antigens on the membrane of infected autologous cells. by comparison, cytotoxicity generated in vitro in an autologous theilerial-lymphocyte culture (autlc) contained both ctl and activity akin to th ... | 1983 | 6187669 |
| incorporation of 3h-hypoxanthine by theileria parva during development in the feeding adult tick rhipicephalus appendiculatus. | 1983 | 6187700 | |
| evidence for a common protective antigenic determinant on sporozoites of several theileria parva strains. | the antigenic relationship amongst sporozoites of several theileria parva strans isolated from different parts of east africa was investigated. it was found that antibodies to t. parva ( muguga ) sporozoites neutralized the infectivity of homologous sporozoites as well as those of t. parva (uganda), t. parva lawrencei , t. parva ( kiambu 5), t. parva ( kabete 1) and t. parva ( marikebuni ). the results correlated with those obtained from an in vivo challenge, in that cattle immunized against t. ... | 1984 | 6203832 |
| evaluation of a range of antimicrobial agents against the parasitic protozoa, plasmodium falciparum, babesia rodhaini and theileria parva in vitro. | eighteen antimicrobials commonly used in tissue culture were screened in three different protozoan test systems in order to establish their suitability for routine inclusion in protozoal cultivation systems. the human malaria parasite, plasmodium falciparum, was inhibited by more than half the antibiotics tested at concentrations recommended for normal tissue culture use. eight compounds were well tolerated and thus could be used prophylactically to prevent microbial contamination. these antimic ... | 1984 | 6206808 |
| detection of antibody to theileria parva schizonts and cell surface membrane antigens of infected lymphoblastoid cells by immunoperoxidase techniques. | peroxidase-labeled antibody procedures were described for detecting bovine antibodies reactive with intracellular theileria parva schizonts and cell surface membrane antigens of infected lymphoblastoid cells. indirect tests were performed where the reacting bovine antibodies were localized with affinity purified rabbit-anti-bovine igg coupled to horseradish peroxidase. a 4- to 8-fold increase in sensitivity for detecting bovine antibodies was obtained with unlabeled rabbit-anti-bovine igg which ... | 1984 | 6208672 |
| the genome of theileria parva: some structural properties. | the dna of the protozoan theileria parva, the causal agent of the bovine east coast fever, has been prepared at least 99% pure from the intra-erythrocytic form of the parasite. its buoyant density was found to be 1.696 g/cm3 and its calculated g + c content was 36.7%. fragmentation of t. parva by the restriction enzyme ecori provides some evidence of the presence of repetitive dna sequences. | 1984 | 6325246 |
| protein antigens of theileria parva macroschizonts and immune precipitation studies. | the proteins of purified macroschizonts from theileria parva, t. lawrencei, and t. taurotragi were analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis. the major proteins of all species had molecular weights of 120,000, 70,000, 65,000, 62,000, 55,000, 44,000, and 35,000. all further experiments were carried out with t. parva. using 125i surface labelling it was established that proteins with molecular weights of 70,000, 50,000, and 44,000 were membrane constituents. staphylococ ... | 1983 | 6402582 |
| rhipicephalus zambeziensis as a vector of bovine theileriae. | rhipicephalus zambeziensis was shown experimentally to transmit theileria parva parva in cattle from nymph to adult and theileria parva lawrencei, theileria parva bovis and theileria taurotragi from larva to nymph and nymph to adult. in a single trial the tick failed to transmit theileria mutans. the tick is believed to be a vector of t. parva lawrencei in the field in zimbabwe. | 1983 | 6405516 |
| morphology of normal and theileria parva-infected salivary glands of rhipicephalus appendiculatus (acari: ixodoidea). | 1983 | 6406660 | |
| infection of mammalian cells with theileria species. | experiments were carried out to determine the susceptibility of mammalian cells to infection with different species of theileria in vitro. sporozoites of theileria parva (parva), theileria parva (lawrencei) and theileria taurotragi were isolated from rhipicephalus appendiculatus ticks by grinding infected ticks in medium, filtering the suspension and concentrating by centrifugation. the sporozoites were used in attempts to infect in vitro peripheral blood leucocytes harvested from 16 different m ... | 1983 | 6406967 |
| transmission of theileria parva by a population of rhipicephalus appendiculatus under simulated natural conditions. | a 2 hectare paddock on the muguga estate, kiambu district of kenya (altitude 2100 m) free of theileria parva-infected ticks was seeded by applying rhipicephalus appendiculatus nymphs to cattle infected with theileria parva (kiambu 4). it was estimated that over 50 000 engorged nymphs fell onto the pasture from 4 cattle with high parasitaemias during the cold season (june). samples of these ticks were placed in plastic cylinders under the vegetation in the paddock and sexual stages of t. parva we ... | 1983 | 6406968 |
| complement activation and fibrinolysis during infection with theileria parva (east coast fever) in cattle. | the role played by complement and fibrinolysis in the pathogenesis of pulmonary oedema and the disseminated, subendothelial, petechial haemorrhages in cattle infected with theileria parva was investigated. there was a marked reduction in total haemolytic complement in animals that died of this disease whereas the survivors showed only transient changes. the reduction in c3 levels in animals with severe disease, however, was equivalent to that in the survivors. neither circulating soluble immune ... | 1983 | 6408786 |
| isoelectric focusing in agarose: a highly discriminatory method for the detection of enzyme heterogeneity. | a comparison was made of the effectiveness of starch gel electrophoresis and isoelectric focusing in agarose for the separation of isoenzymes. lactate dehydrogenase (ldh) of theileria parva and glucose phosphate isomerase (gpi) of various cestodes were examined by the two techniques. isoelectric focusing proved to be highly discriminatory, clearly distinguishing cestode orders and taenia spp. which were closely similar by starch gel electrophoresis, and separating seven bands of theileria-specif ... | 1983 | 6411010 |
| ultrastructural characteristics of in vitro parasite-lymphocyte behaviour in invasions with theileria annulata and theileria parva. | host-parasite relationships have been studied by electron microscopy using glutaraldehyde-oso4-fixed pellets of lymphoid cultures infected in vitro by theileria annulata and t. parva. intracellular presence of the parasite resulted in a progressive and marked lymphoblastoid transformation. the schizont stage periodically provoked the formation of, and adopted an intimate association with, cytoplasmic annulate lamellae in the interphase cell. annulate lamellae developed from the outer nuclear mem ... | 1983 | 6412424 |
| the early events of infection with theileria parva in cattle: infectivity for cattle of leukocytes incubated in vitro with sporozoites. | leukocytes were isolated from bovine blood and, after short periods of incubation in vitro with sporozoites of theileria parva, were washed thoroughly, and their infectivity tested in autologous and allogeneic hosts. using a standard inoculum of 10(6) viable cells, it was found that, after incubation in vitro for either 1 or 24 h, the cells initiated lethal infections in autologous cattle, but failed to infect allogeneic animals. autologous and allogeneic erythrocytes and mouse lymphocytes simil ... | 1983 | 6412425 |
| clinical and diagnostic features of east coast fever (theileria parva) infection of cattle. | east coast fever is a tick-borne protozoal disease affecting cattle in a large part of east and central africa. since the vector occurs over an even wider range there is considerable potential for the disease to spread to countries which are currently disease free. this article, describing the clinical and diagnostic features of east coast fever, may remind authorities in these countries of the potential hazards posed by the disease. | 1983 | 6415893 |
| characterisation of stocks of theileria parva by monoclonal antibody profiles. | sixteen monoclonal antibodies, raised against macroschizonts of theileria parva, were tested against 10 different stocks of the parasite. the indirect fluorescent antibody test was used to demonstrate that these antibodies showed different binding affinities to macroschizonts of the various stocks. a profile of antibody binding could thus be prepared for each stock. for a given stock the profile was consistently the same irrespective of culture passage level, host cell background and method of a ... | 1983 | 6420853 |
| immunisation against east coast fever: correlation between monoclonal antibody profiles of theileria parva stocks and cross immunity in vivo. | stocks of theileria parva, which had been characterised by monoclonal antibody profiles, were used to challenge cattle previously immunised against east coast fever (ecf). when cattle were subjected to homologous challenge, or heterologous challenge with a stock of identical profile to that which had initiated immunity, they showed mild or inapparent reactions. however, when cattle were challenged with a stock of a different profile many underwent severe or fatal ecf reactions. thus, there appea ... | 1983 | 6420854 |
| dose dependent responses of cattle to theileria parva stabilate. | 1984 | 6423557 | |
| some factors controlling the stimulation of sporogony of theileria parva in its tick vector rhipicephalus appendiculatus. | 1984 | 6423558 | |
| cultivation of theileria. i. attempts to complete the cycle of theileria parva in vitro. | microschizonts and free merozoites developed in bovine lymphoblastoid cell cultures containing macroschizonts of 6 different strains of theileria parva. clean bovine red cells were added to the cultures, which were incubated in various ways. no penetration of red cells by merozoites was observed, not even when cultures in diffusion chambers were introduced into the peritoneal cavity of non-infected cattle. | 1984 | 6428031 |
| blast transformation of adherent macrophages infected in vitro with sporozoites of theileria parva. | rhipicephalus appendiculatus ticks were fed on cows with parasitemia from theileria parva infection (east coast fever). homogenates of salivary glands with infective sporozoites were added to cultures of (i) monocyte-derived adherent macrophages from normal bovine peripheral blood and (ii) macrophages from spleen and bone marrow. it was shown by light, phase-contrast, and electron microscopies and by tests for lysosomal enzymes, phagocytosis of carbon particles, and cell markers (fibronectin and ... | 1984 | 6428284 |
| monoclonal antibody neutralizes the sporozoite stage of different theileria parva stocks. | monoclonal antibodies were raised against sporozoites of theileria parva. one of these antibodies (mabd1) neutralized the infectivity of sporozoites for lymphocytes in vitro and for cattle in vivo. neutralization seemed to occur by blocking sporozoite entry into the cell. mabd1 neutralized sporozoites of four unrelated stocks of t. parva, indicating the presence of a common antigenic determinant which may be important in initiating protective immunity. | 1984 | 6433306 |
| titration in cattle of infectivity and immunogenicity of autologous cell lines infected with theileria parva. | cell lines infected with theileria parva were derived by infection of bovine peripheral blood lymphocytes with sporozoites in vitro. cattle were inoculated with doses of autologous infected cells ranging from 1 x 10(1) to 1 x 10(8). infection became established in animals which received 1 x 10(2) or more cells. while 1 x 10(2) cells resulted in sub-patent infection with development of immunity to challenge with sporozoites, larger doses of cells gave rise to patent infections of increasing sever ... | 1984 | 6435303 |
| superinfection of established theileria-infected bovid cell lines with theileria parva sporozoites. | 1984 | 6435553 | |
| chemotherapy of east coast fever: parvaquone treatment of clinical disease induced by isolates of theileria parva. | two experiments were carried out in which parvaquone was used to treat experimentally-induced acute clinical east coast fever infections. in the first experiment, infections with theileria parva parva (kiambu 5) were induced by applying infected rhipicephalus appendiculatus ticks or by inoculation of triturated infected-tick stabilate. the character of the disease was similar with both methods of infection and following a single treatment with parvaquone at 20 mg kg-1, 5 of 7 cattle in each grou ... | 1984 | 6437050 |
| comparison of long acting oxytetracycline and parvaquone in immunisation against east coast fever by infection and treatment. | two groups of five cattle were immunised with a field isolate of theileria parva as stabilate and simultaneously treated with long acting oxytetracycline or parvaquone in early clinical disease. the oxytetracycline group suffered a marked fall in leucocyte count and one animal died during immunisation. the parvaquone group suffered a less severe fall in leucocyte count and all survived. the surviving immunised cattle were immune to homologous challenge but susceptible to subsequent challenge wit ... | 1984 | 6438743 |
| survival of rhipicephalus appendiculatus (acarina: ixodidae) and persistence of theileria parva (apicomplexa: theileriidae) in the field. | 1984 | 6439668 | |
| a rapid method to select rhipicephalus appendiculatus salivary glands infected with theileria parva. | 1984 | 6439849 | |
| interaction of sporozoites of theileria parva with bovine lymphocytes in vitro. i. early events after invasion. | sporozoites of theileria parva rapidly enter bovine lymphocytes by a mechanism of passive endocytosis that depends upon progressive circumferential binding of ligands on the parasite to receptors on the host-cell membrane. within 10 min of entry, the micronemes of the sporozoite discharge their content and the enveloping host-cell membrane is lysed. the host cell responds within 30 min of invasion by polymerization of microtubules arrayed tangential to the sporozoite and converging upon the cyto ... | 1984 | 6442474 |
| generation of autologous mixed leucocyte reactions during the course of infection with theileria parva (east coast fever) in cattle. | during the course of east coast fever (ecf) in cattle, an examination was made of the evolution of autologous mixed leucocyte reactions (mlr) between normal responder lymphocytes collected prior to infection and stimulators cells prepared from infected lymphoid organs at intervals after challenge. proliferative responses were induced by spleen cells, peripheral blood leucocytes (pbl) and cells (lnc) from both regional and distant lymph nodes. the proliferation by normal pbl in response to lnc oc ... | 1980 | 6447668 |
| genetically restricted cell-mediated cytotoxicity in cattle immune to theileria parva. | 1981 | 6451808 | |
| cell-mediated immune responses to theileria parva (east coast fever) during immunization and lethal infections in cattle. | leucocytes from cattle with lethal or sub-lethal infections with theileria parva were examined for responses in autologous mixed lymphocyte reactions (mlr) and for specific and non-specific cytotoxicity against cultured cell lines. during lethal infections, lymphoblasts isolated from central lymph from days 8 to 14 stimulated proliferation in autologous lymphatic lymphocytes and peripheral blood leucocytes (pbl), although cytotoxicity was not generated in such cultures. after day 14, non-specifi ... | 1981 | 6454652 |
| activity of 10 naphthoquinones, including parvaquone (993c) and menoctone, in cattle artificially infected with theileria parva. | ten naphthoquinones, including parvaquone (993c) (clexon; wellcome) and menoctone, were tested for activity in cattle artificially infected with theileria parva, the causative organism of east coast fever (ecf). parvaquone cured all 14 cattle treated with a single dose of 20 mg/kg intramuscularly and all five treated twice with 10 mg/kg intramuscularly. menoctone cured seven of 10 cattle treated with a single dose of 5 mg/kg intramuscularly. of 25 untreated control cattle, 22 died of ecf. none o ... | 1983 | 6665318 |
| a histocompatibility barrier to immunization against east coast fever using theileria parva-infected lymphoblastoid cell lines. | histocompatibility may be a barrier to the infection of cattle when theileria parva parva-infected tissues or in vitro cultured macroschizont-infected lymphoblastoid cell lines are used for immunization. by inoculating 10(3) and 10(5) infected cells into autologous recipients infection was achieved and immunity engendered. cell lines inoculated into bola matched recipients did not produce patent infections but some recipients developed antibodies to the parasite and 3/5 were immune to challenge. ... | 1984 | 6739121 |
| monoclonal antibodies detect antigenic diversity in theileria parva parasites. | 1980 | 6766157 | |
| bovine lymphocyte antigens (bola) of bovine lymphocytes and derived lymphoblastoid lines transformed by theileria parva and theileria annulata. | the bola phenotypes of lymphocytes from seven cattle have been compared with those of 19 lymphoblastoid cell lines derived from them by infection in vitro with either theileria parva or t. annulata. two long-established lines were also studied. in all cases except one, the bola phenotypes of the lymphoblastoid lines were identical with those of the original animal from whose lymphocytes they were derived. the one exception was a chimeric twin where a minor population appeared to have been transf ... | 1980 | 6774305 |
| theileriosis: cell-mediated and humoral immunity. | the protozoan parasite of cattle theileria parva transforms lymphoid cells into permanently proliferating cell lines. antibodies reacting with parasite antigens can be detected by several methods, but there is no evidence that they are protective. two cellular responses to infection have been found. in immune animals powerful cytotoxicity restricted to syngeneic infected cells rapidly appears; this is thought to be mediated by t-lymphocytes and to play a major role in resistance to reinfection. ... | 1981 | 6781356 |
| theileria parva parasites transform a subpopulation of t lymphocytes. | 1981 | 6787130 | |
| the effect of irradiation on the susceptibility of rhipicephalus appendiculatus ticks to theileria parva infection. | irradiation doses of 800 rad. or less had no detectable effect on the subsequent development of unfed nymphal rhipicephalus appendiculatus ticks. doses of 1600 rad. or above impaired feeding and moulting and delayed the subsequent development of unfed nymphs. at 6400 rad. no ticks completed their moult. unfed nymphal r. appendiculatus irradiated at 0, 400, 800 and 1600 rad. were applied to 3 cattle infected with t. parva (kiambu 4). the theileria infections in the salivary glands of the resultan ... | 1981 | 6787541 |
| isoenzyme studies on theileria (protozoa, sporozoa). enzyme activity associated with the erythrocytic stage. | bovine blood containing piroplasms of theileria parva, as well as non-infected blood, was lysed and subjected to iso-electric focussing. staining for 13 different enzymes revealed parasite-associated bands of glucose phosphate isomerase (gpi) activity, not of any of the other enzymes. there were no variations between individual donor animals in the host cell gpi bands and these bands did not interfere with the recognition of the parasite-associated bands, so that purification of the piroplasms w ... | 1981 | 6787731 |
| the fine structural relationship between theileria schizonts and infected bovine lymphoblasts from cultures. | the fine structure of theileria-lymphoblast relationship was studied using cultured bovine lymphoblastoid cells infected with theileria parva, t lawrencei, or t. annulata. the major findings of this study were: (1) the presence of a very active golgi complex with the associated annulate lamelae; (b) the presence of cytoplasmic microtubules which joined the parasites and host cell centriole during lymphoblast mitosis; and (c) the absence of morphological evidence to suggest that the host cell dev ... | 1981 | 6787815 |
| artificial infection of rhipicephalus appendiculatus with theileria parva by percutaneous injection. | rhipicephalus appendiculatus nymphs were inoculated with fresh or cryopreserved blood containing theileria parva piroplasms, or with cell culture grown stages of t parva. the use of fresh blood was successful. cryopreserved blood containing dimethylsulphoxide (dmso), killed most nymphs after inoculation: dmso could be removed by slow dialysis, without destroying the infectivity of the blood. attempts to infect ticks by inoculating cell culture grown stages of t parva failed, even when large numb ... | 1980 | 6789418 |