Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| leishmania infection and blood food sources of phlebotomines in an area of brazil endemic for visceral and tegumentary leishmaniasis. | the aims of the study were to determine the blood feeding preferences of sandflies and to identify species of leishmania that infected phlebotomines in caxias, maranhão, brazil, an area that is highly endemic for leishmaniasis. sandflies were captured in light traps located in the peridomiciliary environments of randomly selected houses in urban and rural settings between 1800 and 0600 hours on new moon days between march 2013 and february 2015. dna extracts from 982 engorged female sandflies we ... | 2017 | 28837565 |
| main lesions in the central nervous system of dogs due to leishmania infantum infection. | canine visceral leishmaniasis (cvl) is endemic in são luís maranhão/brazil and it leads a varied clinical picture, including neurological signs. | 2017 | 28821261 |
| experimental mixed infection of leishmania (leishmania) amazonensis and leishmania (l.) infantum in hamsters (mesocricetus auratus). | in south america, visceral leishmaniasis is frequently caused by leishmania infantum and, at an unknown frequency, by leishmania amazonensis. therefore, mixed infections with these organisms are possible. mixed infections might affect the clinical course, immune response, diagnosis, treatment and epidemiology of the disease. here we describe the clinical course of mixed infections with l. amazonensis and l. infantum in a hamster model. we show that mixed infections are associated with more sever ... | 2017 | 28487000 |
| the use of herbs against neglected diseases: evaluation of in vitro leishmanicidal and trypanocidal activity of stryphnodendron rotundifolium mart. | the evaluation of the leishmanicidal and trypanocidal activity of the hydroalcoholic extract of the bark of stryphnodendron rotundifolium mart. (ehcsr) was carried out to find an alternative treatment for parasitic diseases. ehcsr was prepared and used at four different concentrations (1000, 500, 250, 125 μg/ml) in in vitro assays for activity against leishmania promastigotes using the species leishmania brasiliensis and leishmania infantum and for trypanocidal activity using the epimastigotes o ... | 2017 | 28855804 |
| sensitive molecular diagnostics for cutaneous leishmaniasis. | rapid diagnosis of cutaneous leishmaniasis (cl) and identification of leishmania species is highly important for the disease management. in israel, cl is caused mainly by leishmania major and leishmania tropica species. | 2017 | 28795077 |
| maldi ms imaging investigation of the host response to visceral leishmaniasis. | mass spectrometry imaging (msi) of animal tissues has become an important tool for in situ molecular analyses and biomarker studies in several clinical areas, but there are few applications in parasitological studies. leishmaniasis is a neglected tropical disease, and experimental mouse models have been essential to evaluate pathological and immunological processes and to develop diagnostic methods. herein we have employed maldi msi to examine peptides and low molecular weight proteins (2 to 20 ... | 2017 | 28758666 |
| comparison of recombinant proteins of kinesin 39, heat shock protein 70, heat shock protein 83, and glycoprotein 63 for antibody detection of leishmania martiniquensis infection. | leishmania martiniquensis, a zoonotic hemoflagellate, is a causative agent of cutaneous (cl) and visceral leishmaniasis (vl) among humans and animals. this organism, first reported in martinique island, now has become an emerging infectious agent in thailand. symptomatic cases of l. martiniquensis infection among humans have continuously increased. in the meantime, asymptomatic infection of this novel species has seriously created national public health awareness and concern to prevent and contr ... | 2017 | 28370779 |
| protection mediated by chemokine cxcl10 in balb/c mice infected by leishmania infantum. | visceral leishmaniasis (vl) caused by leishmania infantum is characterised by the loss of the ability of the host to generate an effective immune response. chemokines have a direct involvement in the pathogenesis of leishmaniasis, causing a rapid change in the expression of these molecules during infection by leishmania. | 2017 | 28767981 |
| phlebotomine sand fly survey in the focus of leishmaniasis in madrid, spain (2012-2014): seasonal dynamics, leishmania infantum infection rates and blood meal preferences. | an unusual increase of human leishmaniasis cases due to leishmania infantum is occurring in an urban area of southwestern madrid, spain, since 2010. entomological surveys have shown that phlebotomus perniciosus is the only potential vector. direct xenodiagnosis in hares (lepus granatensis) and rabbits (oryctolagus cuniculus) collected in the focus area proved that they can transmit parasites to colonized p. perniciosus. isolates were characterized as l. infantum. the aim of the present work was ... | 2017 | 28764772 |
| visceral leishmaniasis in southeastern iran: a narrative review. | visceral leishmaniasis (vl) has strong links with poverty, substantial medical and veterinary impacts. this review aimed to focus in studies published during 1994-2016 on vl in southeastern iran. | 2017 | 28761456 |
| multicentre evaluation of a direct agglutination test prototype kit (dat-lpc) for diagnosis of visceral leishmaniasis. | in this study, we assessed the sensitivity, specificity, and diagnostic accuracy of a previously developed direct agglutination test (dat) using a freeze-dried antigen derived from leishmania infantum promastigotes and composed in a prototype kit for visceral leishmaniasis (vl) diagnosis, named dat-lpc. to evaluate dat-lpc reproducibility, the kit was used to analyse 207 serum samples from vl patients and 80 serum samples from patients with other parasitic infections or healthy subjects in four ... | 2017 | 28735574 |
| after infection with leishmania infantum, golden hamsters (mesocricetus auratus) become more attractive to female sand flies (lutzomyia longipalpis). | in brazil, human and canine visceral leishmaniasis is caused by infection with leishmania infantum, a protist parasite transmitted by blood-feeding female lutzomyia longipalpis sand flies. the objective of this study was to determine if the odour of hamsters, infected with le. infantum, was more attractive than the odour of the same hamsters, before they were infected. the attractiveness of odour collected from individual hamsters (n = 13), before they were infected, was compared in a longitudin ... | 2017 | 28733676 |
| antigenicity of phage clones and their synthetic peptides for the serodiagnosis of canine and human visceral leishmaniasis. | in the americas, brazil is responsible by 90% of the cases registered of visceral leishmaniasis (vl), and leishmania infantum is the most common parasite species responsible by disease in brazilian dogs and humans. a precise diagnosis may allow to a faster and more effective treatment against the disease, which increases the possibility of cure, as well as to induce less toxic effects, due to a lower time exposition for the chemotherapeutics. in a previous study, two l. infantum mimotopes, b10 a ... | 2017 | 28629727 |
| vaccination with a leishmania infantum hsp70-ii null mutant confers long-term protective immunity against leishmania major infection in two mice models. | the immunization with genetically attenuated leishmania cell lines has been associated to the induction of memory and effector t cell responses against leishmania able to control subsequent challenges. a leishmania infantum null mutant for the hsp70-ii genes has been described, possessing a non-virulent phenotype. | 2017 | 28558043 |
| insights into the phosphatidylcholine and phosphatidylethanolamine biosynthetic pathways in leishmania parasites and characterization of a choline kinase from leishmania infantum. | the protozoan parasite leishmania infantum is a causative agent of the disease visceral leishmaniasis, which can be fatal if not properly treated. phosphatidylcholine (pc) and phosphatidylethanolamine (pe) biosynthesis pathways are attractive targets for new antileishmanial compounds since these leishmania cell membrane phospholipids are important for parasite morphology and physiology. in this work we observed leishmania synthesize pc and pe from extracellular choline and ethanolamine, respecti ... | 2017 | 28754315 |
| t lymphocytes and macrophages in the intestinal tissues of dogs infected with leishmania infantum. | this study was about a semi-quantitative analysis of t lymphocytes (cd4+ and cd8+, foxp3+ regulatory t cells), and macrophages in the gut wall of dogs naturally infected with leishmania infantum. thirteen dogs were divided into three groups: group 1 (g1, n=5), dogs with canine visceral leishmaniasis (cvl) and infected with l. infantum amastigotes in the intestine; group 2 (g2, n=5), dogs with cvl but without intestinal amastigotes; and group 3 (g3, n=3), uninfected dogs (control group). there wa ... | 2017 | 28746446 |
| spleen damage in a dog naturally infected by leishmania infantum. | 2017 | 28562772 | |
| ecological parameters of the (s)-9-methylgermacrene-b population of the lutzomyia longipalpis complex in a visceral leishmaniasis area in são paulo state, brazil. | visceral leishmaniasis (vl) is an important public health challenge in brazil because of the high number of human and canine cases reported annually. leishmania infantum is the etiological agent of vl and lutzomyia longipalpis is its main vector. however, evidence suggests that this taxon constitutes a species complex. in sao paulo state, there are two populations of lu. longipalpis, each secreting distinct pheromones, (s)-9-methylgermacrene-b and cembrene 1; both have been associated with diffe ... | 2017 | 28558760 |
| antileishmanial activity and tubulin polymerization inhibition of podophyllotoxin derivatives on leishmania infantum. | leishmania microtubules play an important role not only in cell division, but also in keeping the shape of the parasite and motility of its free-living stages. microtubules result from the self-assembly of alpha and beta tubulins, two phylogenetically conserved and very abundant eukaryotic proteins in kinetoplastids. the colchicine binding domain has inspired the discovery and development of several drugs currently in clinical use against parasites. however, this domain is less conserved in kine ... | 2017 | 28719882 |
| occurrence of lutzomyia longipalpis lutz & neiva 1912 and cerdocyon thous linnaeus 1977, in a visceral leishmaniasis endemic area in brazil. | cerdocyon thous presents a wide geographic distribution in brazil and its role as a possible leishmania infantum reservoir in a visceral leishmaniasis (vl) transmission cycle regardless of dogs (canis familiaris) has been discussed. from this perspective, this work describes the occurrence and use of the habitat by cerdocyon thous in a lutzomyia longipalpis occurrence area teresina (piaui - brazil), vl endemic region. three specimens of c. thous were monitored with the use of radio telemetry and ... | 2017 | 28712973 |
| prevention of feline leishmaniosis with an imidacloprid 10%/flumethrin 4.5% polymer matrix collar. | leishmaniosis caused by leishmania infantum is one of the most important vector-borne diseases affecting animals and humans worldwide. dogs are considered main reservoirs of the zoonotic forms, though in the last years the role of cats as reservoirs has been increasingly investigated. feline leishmaniosis (fel) occurs in endemic areas and no specific preventive measures have been investigated so far. in this study the efficacy of a 10% imidacloprid/4.5% flumethrin polymer matrix collar, licensed ... | 2017 | 28705248 |
| standardization and evaluation of a duplex real-time quantitative pcr for the detection of leishmania infantum dna: a sample quality control approach. | molecular techniques have been shown to be alternative methods for the accurate detection of infectious and parasitic diseases, such as the leishmaniases. the present study describes the optimization and evaluation of a duplex real-time quantitative pcr (qpcr) protocol developed for the simultaneous detection of leishmania infantum dna and sample quality control. | 2017 | 28700053 |
| genetic variability of leishmania infantum in naturally infected dogs in the state of bahia, brazil. | in brazil, leishmania infantum mainly affects humans and dogs. the state of bahia presents many dogs that are positive for this parasite. despite the importance of epidemiology in this region, there are still very few studies that have assessed the genetic characteristics of l. infantum. the aim of this study is to investigate the genetic variability of l. infantum isolated identified in naturally infected dogs, in order to verify occurrence of subpopulation of this parasite in the different bio ... | 2017 | 28700001 |
| a nitrile glucoside and biflavones from the leaves of campylospermum excavatum (ochnaceae). | the study of the meoh extract of the leaves of campylospermum excavatum led to the isolation of a nitrile glucoside, named campyloside c (1) and an original derivative of ochnaflavone, 7-o-methylochnaflavone (2) along with three known biflavonoids, amentoflavone, sequoiaflavone, and sotetsuflavone (3-5). the linkage site of the sub-units of 2 was confirmed by chemical correlation, after semi-synthesis of a trimethoxylated derivative of ochnaflavone (2a). the structure of these compounds as well ... | 2017 | 28695668 |
| what pre-columbian mummies could teach us about south american leishmaniases? | a recent report on the taxonomic profile of the human gut microbiome in pre-columbian mummies (santiago-rodriguez et al. 2016) gives for the first time evidence of the presence of leishmania dna (sequences similar to leishmania donovani according to the authors) that can be reminiscent of visceral leishmaniasis during the pre-columbian era. it is commonly assumed that leishmania infantum, the etiological agent of american visceral leishmaniasis (avl) was introduced into the new world by the iber ... | 2017 | 28423167 |
| diagnostic challenges in the era of canine leishmania infantum vaccines. | the diagnosis of canine leishmaniosis (canl) is complex due to its variable clinical manifestations and laboratory findings. the availability of vaccines to prevent canl has increased the complexity of diagnosis, as serological tests may not distinguish between naturally infected and vaccinated dogs. current practices of prevaccination screening are not sufficiently sensitive to detect subclinically infected dogs, resulting in the vaccination of infected animals, which may lead to disease in vac ... | 2017 | 28689776 |
| pegylated dendritic polyglycerol conjugate delivers doxorubicin to the parasitophorous vacuole in leishmania infantum infections. | most drugs against visceral leishmaniasis must be administered parenterally. a controlled drug release at the target site can improve the efficacy and toxicity of antileishmanial drugs in clinical use. amastigotes live and grow inside the parasitophorous vacuole of host resident macrophages. therefore, antileishmanial drugs should accumulate in this compartment to kill the parasite and do not produce toxicity to the cell host. pegylated dendritic polyglycerol conjugates (pg-peg) can ensure a con ... | 2017 | 28683183 |
| environmental factors as key determinants for visceral leishmaniasis in solid organ transplant recipients, madrid, spain. | during a visceral leishmaniasis outbreak in an area of madrid, spain, the incidence of disease among solid organ transplant recipients was 10.3% (7/68). being a black person from sub-saharan africa, undergoing transplantation during the outbreak, and residing <1,000 m from the epidemic focus were risk factors for posttransplant visceral leishmaniasis. | 2017 | 28628447 |
| leishmania infantum is present in vaginal secretions of naturally infected bitches at lower levels in oestrogenized bitches than in non-oestrogenized bitches. | naturally leishmania infantum infected bitches were divided into oestrogenized (n = 11) and non-oestrogenized (n = 6) groups. vaginal secretions were collected for polymerase chain reaction (pcr), and vulval, vaginal and uterine tissues were collected for the immunohistochemical (ihq) identification of l. infantum. parasite dna was identified in vaginal secretions of non-oestrogenized (41.8%) and oestrogenized (18.2%) bitches (p<0.05; fisher's exact test). ihq was positive in vulvar dermis (23.5 ... | 2017 | 28682760 |
| comparative analyses of whole genome sequences of leishmania infantum isolates from humans and dogs in northeastern brazil. | the genomic sequences of 20 leishmania infantum isolates collected in northeastern brazil were compared with each other and with the available genomic sequences of 29 l. infantum/donovani isolates from nepal and turkey. the brazilian isolates were obtained in the early 1990s or since 2009 from patients with visceral or non-ulcerating cutaneous leishmaniasis, asymptomatic humans, or dogs with visceral leishmaniasis. two isolates were from the blood and bone marrow of the same visceral leishmanias ... | 2017 | 28606698 |
| leishmania infantum exoproducts inhibit human invariant nkt cell expansion and activation. | leishmania infantum is one of the major parasite species associated with visceral leishmaniasis, a severe form of the disease that can become lethal if untreated. this obligate intracellular parasite has developed diverse strategies to escape the host immune response, such as exoproducts (exo) carrying a wide range of molecules, including parasite virulence factors, which are potentially implicated in early stages of infection. herein, we report that l. infantum exo and its two fractions compose ... | 2017 | 28674535 |
| a poly(lactic-co-glycolic) acid nanovaccine based on chimeric peptides from different leishmania infantum proteins induces dendritic cells maturation and promotes peptide-specific ifnγ-producing cd8(+) t cells essential for the protection against experimental visceral leishmaniasis. | visceral leishmaniasis, caused by leishmania (l.) donovani and l. infantum protozoan parasites, can provoke overwhelming and protracted epidemics, with high case-fatality rates. an effective vaccine against the disease must rely on the generation of a strong and long-lasting t cell immunity, mediated by cd4(+) th1 and cd8(+) t cells. multi-epitope peptide-based vaccine development is manifesting as the new era of vaccination strategies against leishmania infection. in this study, we designed chi ... | 2017 | 28659922 |
| an ulcer on the lower lip. | 2017 | 28648931 | |
| novel areas for prevention and control of canine leishmaniosis. | there have been multiple recent advances regarding tools for the control and prevention of canine leishmaniosis (canl), including new preventative vaccines. in this review, these advances are evaluated based on control targets, including vector and parasite. leishvet recommendations are provided for control practices based on the dog's risk of infection. new topical insecticide formulations have proven to be effective in preventing sand fly bites, and subsequently infection. parasite control occ ... | 2017 | 28601528 |
| asymptomatic human blood donors carriers of leishmania infantum: potential reservoirs for visceral leishmaniasis in northwestern iran. | little is known regarding transfusion-transmitted leishmaniasis (ttl) and the real global incidence of visceral leishmaniasis (vl) among blood donors as carriers of leishmania spp. in endemic vl foci including iran. recent epidemiological evidences indicate that blood donor may be harbor of leishmania infantum (l. infantum) infection in iran. | 2017 | 28648574 |
| first molecular detection of leishmania infantum in sergentomyia minuta (diptera, psychodidae) in alentejo, southern portugal. | protozoan parasites, such as leishmania spp., are the causative agents of many insect-borne infectious diseases with medical and veterinary importance. leishmaniasis, caused by leishmania spp., is transmitted by female phlebotomine sand flies. in the alentejo region of portugal, located at the north of algarve, cases of human and canine leishmaniasis caused by leishmania infantum have been notified. however, no recent studies regarding the sand fly fauna in the region are available. we therefore ... | 2017 | 28647427 |
| differential activation of human keratinocytes by leishmania species causing localized or disseminated disease. | all leishmania species parasites are introduced into mammalian skin through a sand fly bite, but different species cause distinct clinical outcomes. mouse studies suggest that early responses are critical determinants of subsequent adaptive immunity in leishmaniasis, yet few studies address the role of keratinocytes, the most abundant cell in the epidermis. we hypothesized that leishmania infection causes keratinocytes to produce immunomodulatory factors that influence the outcome of infection. ... | 2017 | 28647347 |
| the performance of serological tests for leishmania infantum infection screening in dogs depends on the prevalence of the disease. | dogs are considered the main reservoir of leishmania infantum. this protozoan causes visceral leishmaniasis (vl), an uncontrolled urban zoonosis in brazil. serological tests and polymerase chain reaction (pcr) on peripheral blood were performed to identify infected dogs in scenarios of higher and lower prevalence of the disease (teresina and vitória). one-hundred infected and 57 non-infected animals from teresina and 100 non-infected animals from vitória were studied. animal selection was not de ... | 2017 | 28591267 |
| new epidemiological pattern of cutaneous leishmaniasis in two pre-saharan arid provinces, southern morocco. | three leishmania species are responsible of cutaneous leishmaniasis (cl) in morocco. zoonotic cl due to leishmania major and leishmania infantum, the first is known as established in the eastern arid regions, whereas the latter evolves sporadically, especially in the north. while leishmania tropica, classically considered anthroponotic, is endemic in the semi-arid regions and is largely distributed throughout the country. the aim of this study was to identify the leishmania species causing cl in ... | 2017 | 28527882 |
| in vivo imaging of pathogen homing to the host tissues. | hematogenous dissemination followed by tissue tropism is a characteristic of the infectious process of many pathogens including those transmitted by blood-feeding vectors. after entering into the blood circulation, these pathogens must arrest in the target organ before they infect a specific tissue. here, we describe a non-invasive method to visualize and quantify the homing of pathogens to the host tissues. by using in vivo bioluminescence imaging we quantify the accumulation of luciferase-expr ... | 2017 | 28522323 |
| lipid bodies accumulation in leishmania infantum-infected c57bl/6 macrophages. | lipid bodies (lbs) are intracellular accumulations of neutral lipids surrounded by a single membrane. these organelles are involved in the production of eicosanoids, which modulate immunity by either promoting or dampening inflammatory responses. leishmania infantum, the etiological agent of visceral leishmaniasis in brazil, is an intracellular parasite that causes disease by suppressing macrophage microbicidal responses. c57bl/6 mouse bone marrow-derived macrophages infected with l. infantum st ... | 2017 | 28518475 |
| geographical information systems in determination of cutaneous leishmaniasis spatial risk level based on distribution of vector species in imamoglu province, adana. | the imamoglu district located in the southeast of adana province in turkey is an endemic focus of cutaneous leishmaniasis (cl) owing to dominancy of phlebotomus tobbi, which is a probable vector of leishmania infantum. about 11.26% of cl cases reported each year are from imamoglu, adana, and between 2008 and 2015, 223 cases of cl were reported. leishmania infantum, which may be transmitted by p. tobbi, phlebotomus neglectus/syriacus, and phlebotomus perfiliewi, is referred as leishmaniasis facto ... | 2017 | 28505264 |
| randomized, allopurinol-controlled trial of the effects of dietary nucleotides and active hexose correlated compound in the treatment of canine leishmaniosis. | first-line treatment for canine leishmaniosis (canl) is n-methylglucamine antimoniate (mga) combined with allopurinol. however, in some dogs allopurinol may induce hyperxanthinuria leading to urolithiasis. moreover, allopurinol resistance has recently been described in leishmania infantum isolates from treated dogs with a relapse of the disease. alternative treatments are thus needed. since the type of host immune response strongly influences canl progression and prognosis, dogs could benefit fr ... | 2017 | 28495197 |
| first report of the in vitro antileishmanial properties of extremophile plants from the algarve coast. | this work reports for the first time the in vitro anti leishmania infantum activity of acetone and dichloromethane (dcm) extracts from 25 extremophile plants from southern portugal. dcm extracts from inula chritmoides and spergularia rubra were active against axenic promastigotes and intracellular amastigotes, had anti-inflammatory properties on lipopolysaccharide (lps)-stimulated macrophages, inhibited acetylcholinesterase and had no haemolytic activity on human erythrocytes. eleven phenolics w ... | 2017 | 28494617 |
| eight cases of cutaneous leishmaniasis due to leishmania infantum with protean presentation. | 2017 | 28492171 | |
| canine β-defensin-1 (cbd1) gene as a possible marker for leishmania infantum infection in dogs. | canine leishmaniasis caused by leishmania infantum is a parasitic disease of great veterinary significance. some dogs infected by l. infantum may mount a strong cellular immune response and clear the infection, while others may respond with exaggerated antibody production against the parasite and develop an overt disease, which may be fatal, if left untreated. the initial factors triggering the polarization of the immune response towards a predominantly t-helper 1 or t-helper 2 cytokines, as wel ... | 2017 | 28427438 |
| a non-pathogenic recombinant leishmania expressing lipophosphoglycan 3 against experimental infection with leishmania infantum. | visceral leishmaniasis (vl) is caused by leishmania infantum in the mediterranean basin and affects primarily children and immunosuppressed individuals. various strategies of vaccination have so far been examined by either protein or dna without achievable complete protection against the disease. the live non-pathogenic lizard parasite, leishmania tarentolae, expressing elected leishmania antigens has recently provided a promising new approach as a safe and effective live vaccine candidate to pr ... | 2017 | 28426153 |
| seroepidemiological survey of human visceral leishmaniasis in ilam province, west of iran in 2013. | visceral leishmaniasis (vl) as one of the most important human parasitic disease is endemic in some parts of iran. several cases of vl have been reported recently in the ilam province. the current study aimed to assess the present status of human vl in the region. | 2017 | 25904946 |
| leishmaniasis in turkey: determination of leishmania species by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (maldi-tof ms). | cutaneous leishmaniasis (cl) is endemic in southeastern anatolia, mainly in sanliurfa and hatay provinces, and the causative agents are mostly leishmania tropica and less frequently l. infantum. here, we report the first maldi-tof analyses of leishmania promastigotes obtained from the cultures of two cl cases from osmaniye and hatay provinces who were initially diagnosed by microscopy, culture and identified as l. infantum with real-time pcr (rt-pcr). | 2017 | 25848391 |
| causes of pediatric visceral leishmaniasis in southeastern iran. | leishmania infantum is the most frequent cause of visceral leishmaniasis and l. tropica has been rarely linked to the disease in iran. in this study, bone marrow aspirates were collected from 10 child patients, suspected with visceral leishmaniasis referred to the pediatric wards of kerman medical hospitals, kerman, iran during 2002-2011. leishmania species were identified by using nested pcr in all slides. the pcr samples from nine patients indicated l. infantum as principal causative agent of ... | 2017 | 25759741 |
| serological detection of toxoplasma gondii, leishmania infantum and neospora caninum in cats from an area endemic for leishmaniasis in brazil. | an investigation was made into the occurrence of antibodies to toxoplasma gondii, leishmania infantum and neospora caninum in 151 domestic cats, based on the indirect fluorescent antibody test (ifat). serum samples were collected from 151 domestic cats (65 free-roaming and 86 domiciled cats; 55 males and 96 females) in campo grande, mato grosso do sul, brazil between january and april 2013. igg antibodies to t. gondii, l. infantum and n. caninum were found, respectively, in 49 (32.5%), 34 (22.5% ... | 2017 | 25517522 |
| mucocutaneous leishmaniasis caused by leishmania infantum var lombardi in an immunocompetent patient, spain. | 2017 | 25559703 | |
| molecular characterization of leishmania infantum strains by kinetoplast dna rflp-pcr. | multilocus enzyme electrophoresis is the tool most frequently used to classify leishmania spp., although it is time consuming and, sometimes, a not enough discriminative method. in the present study a kinetoplast dna polymerase chain reaction-restriction fragment length polymorphism (pcr-rflp) was used to characterize 16 zymodeme mon-1 leishmania infantum strains: 15 were from dogs housed in public kennels of 7 geographical areas in the emilia-romagna region, northern italy, 1 was the l. infantu ... | 2017 | 27033533 |
| antiprotozoal activity of major constituents from the bioactive fraction of verbesina encelioides. | the bioactive petroleum ether fraction of verbesina encelioides, previously studied by the authors, was chosen for the isolation of antiprotozoal metabolites. pseudotaraxasterol-3β-acetate (1), benzyl 2,6-dimethoxy benzoate (2), 16β-hydroxy-pseudotaraxasterol-3β-palmitate (3) and pseudotaraxasterol (4), in addition to β-sitosterol glucoside (5) and β-sitosterol galactoside (6) were isolated and identified based on one-dimensional and two-dimensional spectral analysis. this is the first report de ... | 2017 | 27154232 |
| leishmania infantum antigens modulate memory cell subsets of liver resident t lymphocyte. | in the recent years, the liver has been recognized as an important immune organ with major regulatory functions and immune memory, adding to the well-described vital metabolic functions. there are evidences from experimental infections performed with visceral leishmania species that immune responses to parasite infection can be organ-specific. the liver is the compartment of acute resolving infection, with minimal tissue damage and resistance to reinfection, whereas the spleen is the compartment ... | 2017 | 27615509 |
| canine visceral leishmaniasis in iran: a systematic review and meta-analysis. | visceral leishmaniasis is considered an endemic zoonosis in some parts of iran and dogs are main reservoirs, which play role in the transmission cycle of human leishmaniasis. this systematic review and meta-analysis was performed to determine the prevalence of canine visceral leishmaniasis (cvl) in iran. data were systematically collected from 1982 to 2015 in iran on the following electronic databases: pubmed, google scholar, science direct, scopus, web of science, magiran, irandoc, iran medex a ... | 2017 | 27570207 |
| biological activities of triterpenoids from poraqueiba sericea stems. | eleven compounds were isolated from poraqueiba sericea stems and identified as niga-ichigoside-f1 (1), trachelosperoside b1 (2), 4-epi-niga-ichigoside (7), 19α-hydroxyasiatic acid (3), myrianthic acid (4), hyptatic acid (5), trachelosperogenin b (6), arjunolic acid (8), and trachelosperogenin e (9), secologanoside (10) and secoxyloganin (11). compounds 1-11 were tested for their antileishmanial activities against leishmania infantum promastigotes, 1-6 and 8-11 were tested for their cytotoxic act ... | 2017 | 27736194 |
| the challenge of stability in high-throughput gene expression analysis: comprehensive selection and evaluation of reference genes for balb/c mice spleen samples in the leishmania infantum infection model. | the interaction of leishmania with balb/c mice induces dramatic changes in transcriptome patterns in the parasite, but also in the target organs (spleen, liver…) due to its response against infection. real-time quantitative pcr (qpcr) is an interesting approach to analyze these changes and understand the immunological pathways that lead to protection or progression of disease. however, qpcr results need to be normalized against one or more reference genes (rg) to correct for non-specific experim ... | 2017 | 27668434 |
| the recombinant protein rsp03b is a valid antigen for screening dog exposure to phlebotomus perniciosus across foci of canine leishmaniasis. | the frequency of sandfly-host contacts can be measured by host antibody levels against sandfly salivary proteins. recombinant salivary proteins are suggested to represent a valid replacement for salivary gland homogenate (sgh); however, it is necessary to prove that such antigens are recognized by antibodies against various populations of the same species. phlebotomus perniciosus (diptera: psychodidae) is the main vector of leishmania infantum (trypanosomatida: trypanosomatidae) in southwest eur ... | 2017 | 27718267 |
| novel gold(i) complexes with 5-phenyl-1,3,4-oxadiazole-2-thione and phosphine as potential anticancer and antileishmanial agents. | the current anticancer and antileishmanial drug arsenal presents several limitations concerning their specificity, efficacy, costs and the emergence of drug-resistant cells lines, which encourages the urgent need to search for new alternatives. inspired by the fact that gold(i)-based compounds are promising antitumoral and antileishmanial drug candidates, we synthesized novel gold(i) complexes containing phosphine and 5-phenyl-1,3,4-oxadiazole-2-thione and evaluated their anticancer and antileis ... | 2017 | 27823888 |
| in vitro antileishmanial and antimalarial activity of selected plants of nepal. | nepal is very rich in biodiversity, and no extensive effort has yet been carried out to screen plants that are used by traditional healers against parasitic diseases. the aim of this study was to evaluate the in vitro antileishmanial and antimalarial activity of crude methanolic or ethanolic extracts of 29 plant species that are currently used by local people of nepal for treating different ailments. | 2017 | 27757268 |
| a recombinant chimeric protein composed of human and mice-specific cd4(+) and cd8(+) t-cell epitopes protects against visceral leishmaniasis. | in this study, a recombinant chimeric protein (rcp), which was composed of specific cd4(+) and cd8(+) t-cell epitopes to murine and human haplotypes, was evaluated as an immunogen against leishmania infantum infection in a murine model. balb/c mice received saline were immunized with saponin or with rcp with or without an adjuvant. the results showed that rcp/saponin-vaccinated mice presented significantly higher levels of antileishmanial ifn-γ, il-12 and gm-csf before and after challenge, which ... | 2017 | 27593711 |
| insights on adaptive and innate immunity in canine leishmaniosis. | canine leishmaniosis (canl) is caused by the parasite leishmania infantum and is a systemic disease, which can present with variable clinical signs, and clinicopathological abnormalities. clinical manifestations can range from subclinical infection to very severe systemic disease. leishmaniosis is categorized as a neglected tropical disease and the complex immune responses associated with leishmania species makes therapeutic treatments and vaccine development challenging for both dogs and humans ... | 2017 | 27094260 |
| immune response to recombinant leishmania infantum lipophosphoglycan 3 plus cpg oligodeoxynucleotides in balb/c mice. | development of a protective antileishmanial vaccine is an urgent priority for successful control of different forms of leishmaniasis. the potential of a recombinant lipophosphoglycan 3 (rlpg3) expressed by leishmania tarentolae was evaluated in combination with cpg oligodeoxynucleotides (cpg-odn) as a th1-promoting adjuvant against leishmania infantum infection in balb/c mice. first, mice were immunized subcutaneously with rlpg3 either alone or in combination with cpg-odn. next, the immunogenic ... | 2017 | 27353355 |
| canine antibody response to lutzomyia longipalpis saliva in endemic area of visceral leishmaniasis. | canine exposure to lutzomyia longipalpis bites and the potential of leishmania infantum transmissibility for the vector were evaluated. | 2017 | 27384835 |