Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| first case of feline leishmaniosis caused by leishmania infantum genotype e in a cat with a concurrent nasal squamous cell carcinoma. | this is the first clinical report of feline viscerocutaneous leishmaniosis caused by leishmania infantum genotype e associated with an invasive squamous cell carcinoma (scc) in a domestic cat from portugal. initially, the cat presented a single cutaneous lesion in the right nostril. a fine-needle aspiration was performed and leishmania amastigotes were observed without the presence of cells compatible with neoplasia. systemic treatment with allopurinol was started. one year later, the cat presen ... | 2017 | 28491373 |
| eight cases of cutaneous leishmaniasis due to leishmania infantum with protean presentation. | 2017 | 28492171 | |
| first report of the in vitro antileishmanial properties of extremophile plants from the algarve coast. | this work reports for the first time the in vitro anti leishmania infantum activity of acetone and dichloromethane (dcm) extracts from 25 extremophile plants from southern portugal. dcm extracts from inula chritmoides and spergularia rubra were active against axenic promastigotes and intracellular amastigotes, had anti-inflammatory properties on lipopolysaccharide (lps)-stimulated macrophages, inhibited acetylcholinesterase and had no haemolytic activity on human erythrocytes. eleven phenolics w ... | 2017 | 28494617 |
| randomized, allopurinol-controlled trial of the effects of dietary nucleotides and active hexose correlated compound in the treatment of canine leishmaniosis. | first-line treatment for canine leishmaniosis (canl) is n-methylglucamine antimoniate (mga) combined with allopurinol. however, in some dogs allopurinol may induce hyperxanthinuria leading to urolithiasis. moreover, allopurinol resistance has recently been described in leishmania infantum isolates from treated dogs with a relapse of the disease. alternative treatments are thus needed. since the type of host immune response strongly influences canl progression and prognosis, dogs could benefit fr ... | 2017 | 28495197 |
| zoonotic intestinal parasites and vector-borne pathogens in italian shelter and kennel dogs. | this study investigated the presence of zoonotic parasites and vector-borne pathogens in dogs housed in kennels and shelters from four sites of italy. a total of 150 adoptable dogs was examined with different microscopic, serological and molecular methods. overall 129 dogs (86%) were positive for one or more parasites and/or pathogens transmitted by ectoparasites. forty-eight (32%) were positive for one infection, while 81 (54%) for more than one pathogen. the most common zoonotic helminths reco ... | 2017 | 28504099 |
| combined treatment of miltefosine and paromomycin delays the onset of experimental drug resistance in leishmania infantum. | since miltefosine monotherapy against visceral leishmaniasis (vl) caused by leishmania donovani has been discontinued in the indian subcontinent due to an increase in the number of treatment failures, single dose liposomal amphotericin b is now advocated as a treatment option of choice. paromomycin-miltefosine combination therapy can be used as substitute first-line treatment in regions without cold-chain potential. previous laboratory studies in the closely related species leishmania infantum h ... | 2017 | 28505185 |
| geographical information systems in determination of cutaneous leishmaniasis spatial risk level based on distribution of vector species in imamoglu province, adana. | the imamoglu district located in the southeast of adana province in turkey is an endemic focus of cutaneous leishmaniasis (cl) owing to dominancy of phlebotomus tobbi, which is a probable vector of leishmania infantum. about 11.26% of cl cases reported each year are from imamoglu, adana, and between 2008 and 2015, 223 cases of cl were reported. leishmania infantum, which may be transmitted by p. tobbi, phlebotomus neglectus/syriacus, and phlebotomus perfiliewi, is referred as leishmaniasis facto ... | 2017 | 28505264 |
| lipid bodies accumulation in leishmania infantum-infected c57bl/6 macrophages. | lipid bodies (lbs) are intracellular accumulations of neutral lipids surrounded by a single membrane. these organelles are involved in the production of eicosanoids, which modulate immunity by either promoting or dampening inflammatory responses. leishmania infantum, the etiological agent of visceral leishmaniasis in brazil, is an intracellular parasite that causes disease by suppressing macrophage microbicidal responses. c57bl/6 mouse bone marrow-derived macrophages infected with l. infantum st ... | 2017 | 28518475 |
| in vivo imaging of pathogen homing to the host tissues. | hematogenous dissemination followed by tissue tropism is a characteristic of the infectious process of many pathogens including those transmitted by blood-feeding vectors. after entering into the blood circulation, these pathogens must arrest in the target organ before they infect a specific tissue. here, we describe a non-invasive method to visualize and quantify the homing of pathogens to the host tissues. by using in vivo bioluminescence imaging we quantify the accumulation of luciferase-expr ... | 2017 | 28522323 |
| new epidemiological pattern of cutaneous leishmaniasis in two pre-saharan arid provinces, southern morocco. | three leishmania species are responsible of cutaneous leishmaniasis (cl) in morocco. zoonotic cl due to leishmania major and leishmania infantum, the first is known as established in the eastern arid regions, whereas the latter evolves sporadically, especially in the north. while leishmania tropica, classically considered anthroponotic, is endemic in the semi-arid regions and is largely distributed throughout the country. the aim of this study was to identify the leishmania species causing cl in ... | 2017 | 28527882 |
| prediction and analysis of promiscuous t cell-epitopes derived from the vaccine candidate antigens of leishmania donovani binding to mhc class-ii alleles using in silico approach. | visceral leishmaniasis is a dreadful infectious disease and caused by the intracellular protozoan parasites, leishmania donovani and leishmania infantum. despite extensive efforts for developing effective prophylactic vaccine, still no vaccine is available against leishmaniasis. however, advancement in immunoinformatics methods generated new dimension in peptide based vaccine development. the present study was aimed to identify t-cell epitopes from the vaccine candidate antigens like lipophospho ... | 2017 | 28549876 |
| vaccination with a leishmania infantum hsp70-ii null mutant confers long-term protective immunity against leishmania major infection in two mice models. | the immunization with genetically attenuated leishmania cell lines has been associated to the induction of memory and effector t cell responses against leishmania able to control subsequent challenges. a leishmania infantum null mutant for the hsp70-ii genes has been described, possessing a non-virulent phenotype. | 2017 | 28558043 |
| ecological parameters of the (s)-9-methylgermacrene-b population of the lutzomyia longipalpis complex in a visceral leishmaniasis area in são paulo state, brazil. | visceral leishmaniasis (vl) is an important public health challenge in brazil because of the high number of human and canine cases reported annually. leishmania infantum is the etiological agent of vl and lutzomyia longipalpis is its main vector. however, evidence suggests that this taxon constitutes a species complex. in sao paulo state, there are two populations of lu. longipalpis, each secreting distinct pheromones, (s)-9-methylgermacrene-b and cembrene 1; both have been associated with diffe ... | 2017 | 28558760 |
| spleen damage in a dog naturally infected by leishmania infantum. | 2017 | 28562772 | |
| detection and identification of leishmania spp.: application of two hsp70-based pcr-rflp protocols to clinical samples from the new world. | leishmaniasis is highly prevalent in new world countries, where several methods are available for detection and identification of leishmania spp. two hsp70-based pcr protocols (pcr-n and pcr-f) and their corresponding restriction fragment length polymorphisms (rflp) were applied for detection and identification of leishmania spp. in clinical samples recruited in colombia, guatemala, and honduras. a total of 93 cases were studied. the samples were classified into positive or suspected of leishman ... | 2017 | 28573463 |
| molecular signatures of neutrophil extracellular traps in human visceral leishmaniasis. | infections with parasites of the leishmania donovani complex result in clinical outcomes that range from asymptomatic infection to severe and fatal visceral leishmaniasis (vl). neutrophils are major players of the immune response against leishmania, but their contribution to distinct states of infection is unknown. gene expression data suggest the activation of the netosis pathway during human visceral leishmaniasis. thus, we conducted an exploratory study to evaluate net-related molecules in re ... | 2017 | 28583201 |
| leishmaniasis in turkey: visceral and cutaneous leishmaniasis caused by leishmania donovani in turkey. | in turkey, the main causative agents are leishmania tropica (l. tropica) and leishmania infantum (l. infantum) for cutaneous leishmaniasis (cl) and l. infantum for visceral leishmaniasis (vl). in this study, we investigated leishmaniasis cases caused by l. donovani and established animal models for understanding its tropism in in vivo conditions. clinical samples (lesion aspirations and bone marrow) obtained from cl/vl patients were investigated using parasitological (smear/nnn) and dna-based te ... | 2017 | 28587839 |
| the performance of serological tests for leishmania infantum infection screening in dogs depends on the prevalence of the disease. | dogs are considered the main reservoir of leishmania infantum. this protozoan causes visceral leishmaniasis (vl), an uncontrolled urban zoonosis in brazil. serological tests and polymerase chain reaction (pcr) on peripheral blood were performed to identify infected dogs in scenarios of higher and lower prevalence of the disease (teresina and vitória). one-hundred infected and 57 non-infected animals from teresina and 100 non-infected animals from vitória were studied. animal selection was not de ... | 2017 | 28591267 |
| novel areas for prevention and control of canine leishmaniosis. | there have been multiple recent advances regarding tools for the control and prevention of canine leishmaniosis (canl), including new preventative vaccines. in this review, these advances are evaluated based on control targets, including vector and parasite. leishvet recommendations are provided for control practices based on the dog's risk of infection. new topical insecticide formulations have proven to be effective in preventing sand fly bites, and subsequently infection. parasite control occ ... | 2017 | 28601528 |
| comparative analyses of whole genome sequences of leishmania infantum isolates from humans and dogs in northeastern brazil. | the genomic sequences of 20 leishmania infantum isolates collected in northeastern brazil were compared with each other and with the available genomic sequences of 29 l. infantum/donovani isolates from nepal and turkey. the brazilian isolates were obtained in the early 1990s or since 2009 from patients with visceral or non-ulcerating cutaneous leishmaniasis, asymptomatic humans, or dogs with visceral leishmaniasis. two isolates were from the blood and bone marrow of the same visceral leishmanias ... | 2017 | 28606698 |
| ifn-γ, il-2, ip-10, and mig as biomarkers of exposure to leishmania spp., and of cure in human visceral leishmaniasis. | new biomarkers are needed for monitoring the effectiveness of treatment for visceral leishmaniasis (vl). they might also improve the detection of the asymptomatic population in leishmania-endemic areas. this paper examines the il-2, ifn-γ, ifn-γ-induced protein 10 (ip-10), and monokine-induced-by-ifn-γ (mig) levels in whole blood-stimulated in vitro with soluble leishmania antigen (sla)-taken from asymptomatic individuals and patients treated for vl living in a post-outbreak (leishmania infantum ... | 2017 | 28620584 |
| environmental factors as key determinants for visceral leishmaniasis in solid organ transplant recipients, madrid, spain. | during a visceral leishmaniasis outbreak in an area of madrid, spain, the incidence of disease among solid organ transplant recipients was 10.3% (7/68). being a black person from sub-saharan africa, undergoing transplantation during the outbreak, and residing <1,000 m from the epidemic focus were risk factors for posttransplant visceral leishmaniasis. | 2017 | 28628447 |
| the current status of phlebotomine sand flies in albania and incrimination of phlebotomus neglectus (diptera, psychodidae) as the main vector of leishmania infantum. | the incidence of visceral leishmaniasis (vl) in albania is higher than in other countries of southern europe, however the role of local sand fly species in the transmission of leishmania infantum was not addressed conclusively. in 2006, a country-wide collection of sand flies performed in 14 sites selected based on recent occurrence of vl cases showed that phlebotomus neglectus was by far the most prevalent species (95.6%). furthermore, 15% of pools made from 422 p. neglectus females tested posi ... | 2017 | 28628627 |
| antigenicity of phage clones and their synthetic peptides for the serodiagnosis of canine and human visceral leishmaniasis. | in the americas, brazil is responsible by 90% of the cases registered of visceral leishmaniasis (vl), and leishmania infantum is the most common parasite species responsible by disease in brazilian dogs and humans. a precise diagnosis may allow to a faster and more effective treatment against the disease, which increases the possibility of cure, as well as to induce less toxic effects, due to a lower time exposition for the chemotherapeutics. in a previous study, two l. infantum mimotopes, b10 a ... | 2017 | 28629727 |
| differential activation of human keratinocytes by leishmania species causing localized or disseminated disease. | all leishmania species parasites are introduced into mammalian skin through a sand fly bite, but different species cause distinct clinical outcomes. mouse studies suggest that early responses are critical determinants of subsequent adaptive immunity in leishmaniasis, yet few studies address the role of keratinocytes, the most abundant cell in the epidermis. we hypothesized that leishmania infection causes keratinocytes to produce immunomodulatory factors that influence the outcome of infection. ... | 2017 | 28647347 |
| first molecular detection of leishmania infantum in sergentomyia minuta (diptera, psychodidae) in alentejo, southern portugal. | protozoan parasites, such as leishmania spp., are the causative agents of many insect-borne infectious diseases with medical and veterinary importance. leishmaniasis, caused by leishmania spp., is transmitted by female phlebotomine sand flies. in the alentejo region of portugal, located at the north of algarve, cases of human and canine leishmaniasis caused by leishmania infantum have been notified. however, no recent studies regarding the sand fly fauna in the region are available. we therefore ... | 2017 | 28647427 |
| asymptomatic human blood donors carriers of leishmania infantum: potential reservoirs for visceral leishmaniasis in northwestern iran. | little is known regarding transfusion-transmitted leishmaniasis (ttl) and the real global incidence of visceral leishmaniasis (vl) among blood donors as carriers of leishmania spp. in endemic vl foci including iran. recent epidemiological evidences indicate that blood donor may be harbor of leishmania infantum (l. infantum) infection in iran. | 2017 | 28648574 |
| an ulcer on the lower lip. | 2017 | 28648931 | |
| a poly(lactic-co-glycolic) acid nanovaccine based on chimeric peptides from different leishmania infantum proteins induces dendritic cells maturation and promotes peptide-specific ifnγ-producing cd8(+) t cells essential for the protection against experimental visceral leishmaniasis. | visceral leishmaniasis, caused by leishmania (l.) donovani and l. infantum protozoan parasites, can provoke overwhelming and protracted epidemics, with high case-fatality rates. an effective vaccine against the disease must rely on the generation of a strong and long-lasting t cell immunity, mediated by cd4(+) th1 and cd8(+) t cells. multi-epitope peptide-based vaccine development is manifesting as the new era of vaccination strategies against leishmania infection. in this study, we designed chi ... | 2017 | 28659922 |
| leishmania infantum exoproducts inhibit human invariant nkt cell expansion and activation. | leishmania infantum is one of the major parasite species associated with visceral leishmaniasis, a severe form of the disease that can become lethal if untreated. this obligate intracellular parasite has developed diverse strategies to escape the host immune response, such as exoproducts (exo) carrying a wide range of molecules, including parasite virulence factors, which are potentially implicated in early stages of infection. herein, we report that l. infantum exo and its two fractions compose ... | 2017 | 28674535 |
| leishmania infantum is present in vaginal secretions of naturally infected bitches at lower levels in oestrogenized bitches than in non-oestrogenized bitches. | naturally leishmania infantum infected bitches were divided into oestrogenized (n = 11) and non-oestrogenized (n = 6) groups. vaginal secretions were collected for polymerase chain reaction (pcr), and vulval, vaginal and uterine tissues were collected for the immunohistochemical (ihq) identification of l. infantum. parasite dna was identified in vaginal secretions of non-oestrogenized (41.8%) and oestrogenized (18.2%) bitches (p<0.05; fisher's exact test). ihq was positive in vulvar dermis (23.5 ... | 2017 | 28682760 |
| in vitro effects of purine and pyrimidine analogues on leishmania donovani and leishmania infantum promastigotes and intracellular amastigotes. | inhibition of parasite metabolic pathways is a rationale for new chemotherapeutic strategies. the pyrimidine and purine salvage pathways are thus targets against leishmania donovani and l. infantum, causative agents of visceral human leishmaniasis and canine leishmaniosis. the antiproliferative effect of the pyrimidine analogues cytarabine and 5-fluorouracil and of the purine analogues azathioprine and 6-mercaptopurine was evaluated in vitro on the promastigote and the intracellular amastigote s ... | 2017 | 28682767 |
| pegylated dendritic polyglycerol conjugate delivers doxorubicin to the parasitophorous vacuole in leishmania infantum infections. | most drugs against visceral leishmaniasis must be administered parenterally. a controlled drug release at the target site can improve the efficacy and toxicity of antileishmanial drugs in clinical use. amastigotes live and grow inside the parasitophorous vacuole of host resident macrophages. therefore, antileishmanial drugs should accumulate in this compartment to kill the parasite and do not produce toxicity to the cell host. pegylated dendritic polyglycerol conjugates (pg-peg) can ensure a con ... | 2017 | 28683183 |
| diagnostic challenges in the era of canine leishmania infantum vaccines. | the diagnosis of canine leishmaniosis (canl) is complex due to its variable clinical manifestations and laboratory findings. the availability of vaccines to prevent canl has increased the complexity of diagnosis, as serological tests may not distinguish between naturally infected and vaccinated dogs. current practices of prevaccination screening are not sufficiently sensitive to detect subclinically infected dogs, resulting in the vaccination of infected animals, which may lead to disease in vac ... | 2017 | 28689776 |
| a nitrile glucoside and biflavones from the leaves of campylospermum excavatum (ochnaceae). | the study of the meoh extract of the leaves of campylospermum excavatum led to the isolation of a nitrile glucoside, named campyloside c (1) and an original derivative of ochnaflavone, 7-o-methylochnaflavone (2) along with three known biflavonoids, amentoflavone, sequoiaflavone, and sotetsuflavone (3-5). the linkage site of the sub-units of 2 was confirmed by chemical correlation, after semi-synthesis of a trimethoxylated derivative of ochnaflavone (2a). the structure of these compounds as well ... | 2017 | 28695668 |
| genetic variability of leishmania infantum in naturally infected dogs in the state of bahia, brazil. | in brazil, leishmania infantum mainly affects humans and dogs. the state of bahia presents many dogs that are positive for this parasite. despite the importance of epidemiology in this region, there are still very few studies that have assessed the genetic characteristics of l. infantum. the aim of this study is to investigate the genetic variability of l. infantum isolated identified in naturally infected dogs, in order to verify occurrence of subpopulation of this parasite in the different bio ... | 2017 | 28700001 |
| standardization and evaluation of a duplex real-time quantitative pcr for the detection of leishmania infantum dna: a sample quality control approach. | molecular techniques have been shown to be alternative methods for the accurate detection of infectious and parasitic diseases, such as the leishmaniases. the present study describes the optimization and evaluation of a duplex real-time quantitative pcr (qpcr) protocol developed for the simultaneous detection of leishmania infantum dna and sample quality control. | 2017 | 28700053 |
| prevention of feline leishmaniosis with an imidacloprid 10%/flumethrin 4.5% polymer matrix collar. | leishmaniosis caused by leishmania infantum is one of the most important vector-borne diseases affecting animals and humans worldwide. dogs are considered main reservoirs of the zoonotic forms, though in the last years the role of cats as reservoirs has been increasingly investigated. feline leishmaniosis (fel) occurs in endemic areas and no specific preventive measures have been investigated so far. in this study the efficacy of a 10% imidacloprid/4.5% flumethrin polymer matrix collar, licensed ... | 2017 | 28705248 |
| occurrence of lutzomyia longipalpis lutz & neiva 1912 and cerdocyon thous linnaeus 1977, in a visceral leishmaniasis endemic area in brazil. | cerdocyon thous presents a wide geographic distribution in brazil and its role as a possible leishmania infantum reservoir in a visceral leishmaniasis (vl) transmission cycle regardless of dogs (canis familiaris) has been discussed. from this perspective, this work describes the occurrence and use of the habitat by cerdocyon thous in a lutzomyia longipalpis occurrence area teresina (piaui - brazil), vl endemic region. three specimens of c. thous were monitored with the use of radio telemetry and ... | 2017 | 28712973 |
| antileishmanial activity and tubulin polymerization inhibition of podophyllotoxin derivatives on leishmania infantum. | leishmania microtubules play an important role not only in cell division, but also in keeping the shape of the parasite and motility of its free-living stages. microtubules result from the self-assembly of alpha and beta tubulins, two phylogenetically conserved and very abundant eukaryotic proteins in kinetoplastids. the colchicine binding domain has inspired the discovery and development of several drugs currently in clinical use against parasites. however, this domain is less conserved in kine ... | 2017 | 28719882 |
| after infection with leishmania infantum, golden hamsters (mesocricetus auratus) become more attractive to female sand flies (lutzomyia longipalpis). | in brazil, human and canine visceral leishmaniasis is caused by infection with leishmania infantum, a protist parasite transmitted by blood-feeding female lutzomyia longipalpis sand flies. the objective of this study was to determine if the odour of hamsters, infected with le. infantum, was more attractive than the odour of the same hamsters, before they were infected. the attractiveness of odour collected from individual hamsters (n = 13), before they were infected, was compared in a longitudin ... | 2017 | 28733676 |
| multicentre evaluation of a direct agglutination test prototype kit (dat-lpc) for diagnosis of visceral leishmaniasis. | in this study, we assessed the sensitivity, specificity, and diagnostic accuracy of a previously developed direct agglutination test (dat) using a freeze-dried antigen derived from leishmania infantum promastigotes and composed in a prototype kit for visceral leishmaniasis (vl) diagnosis, named dat-lpc. to evaluate dat-lpc reproducibility, the kit was used to analyse 207 serum samples from vl patients and 80 serum samples from patients with other parasitic infections or healthy subjects in four ... | 2017 | 28735574 |
| t lymphocytes and macrophages in the intestinal tissues of dogs infected with leishmania infantum. | this study was about a semi-quantitative analysis of t lymphocytes (cd4+ and cd8+, foxp3+ regulatory t cells), and macrophages in the gut wall of dogs naturally infected with leishmania infantum. thirteen dogs were divided into three groups: group 1 (g1, n=5), dogs with canine visceral leishmaniasis (cvl) and infected with l. infantum amastigotes in the intestine; group 2 (g2, n=5), dogs with cvl but without intestinal amastigotes; and group 3 (g3, n=3), uninfected dogs (control group). there wa ... | 2017 | 28746446 |
| f1 domain of the leishmania (leishmania) donovani nucleoside hydrolase promotes a th1 response in leishmania (leishmania) infantum cured patients and in asymptomatic individuals living in an endemic area of leishmaniasis. | the leishmania (leishmania) donovani nucleoside hydrolase nh36 is the main antigen of the leishmune(®) vaccine and one of the promising candidates for vaccination against visceral leishmaniasis. the antigenicity of the n-terminal (f1), the central (f2), or the c-terminal recombinant domain (f3) of nh36 was evaluated using peripheral blood mononuclear cells (pbmc) from individuals infected with l. (l.) infantum from an endemic area of visceral leishmaniasis of spain. both nh36 and f1 domains sign ... | 2017 | 28747911 |
| insights into the phosphatidylcholine and phosphatidylethanolamine biosynthetic pathways in leishmania parasites and characterization of a choline kinase from leishmania infantum. | the protozoan parasite leishmania infantum is a causative agent of the disease visceral leishmaniasis, which can be fatal if not properly treated. phosphatidylcholine (pc) and phosphatidylethanolamine (pe) biosynthesis pathways are attractive targets for new antileishmanial compounds since these leishmania cell membrane phospholipids are important for parasite morphology and physiology. in this work we observed leishmania synthesize pc and pe from extracellular choline and ethanolamine, respecti ... | 2017 | 28754315 |
| maldi ms imaging investigation of the host response to visceral leishmaniasis. | mass spectrometry imaging (msi) of animal tissues has become an important tool for in situ molecular analyses and biomarker studies in several clinical areas, but there are few applications in parasitological studies. leishmaniasis is a neglected tropical disease, and experimental mouse models have been essential to evaluate pathological and immunological processes and to develop diagnostic methods. herein we have employed maldi msi to examine peptides and low molecular weight proteins (2 to 20 ... | 2017 | 28758666 |
| visceral leishmaniasis in southeastern iran: a narrative review. | visceral leishmaniasis (vl) has strong links with poverty, substantial medical and veterinary impacts. this review aimed to focus in studies published during 1994-2016 on vl in southeastern iran. | 2017 | 28761456 |
| phlebotomine sand fly survey in the focus of leishmaniasis in madrid, spain (2012-2014): seasonal dynamics, leishmania infantum infection rates and blood meal preferences. | an unusual increase of human leishmaniasis cases due to leishmania infantum is occurring in an urban area of southwestern madrid, spain, since 2010. entomological surveys have shown that phlebotomus perniciosus is the only potential vector. direct xenodiagnosis in hares (lepus granatensis) and rabbits (oryctolagus cuniculus) collected in the focus area proved that they can transmit parasites to colonized p. perniciosus. isolates were characterized as l. infantum. the aim of the present work was ... | 2017 | 28764772 |
| protection mediated by chemokine cxcl10 in balb/c mice infected by leishmania infantum. | visceral leishmaniasis (vl) caused by leishmania infantum is characterised by the loss of the ability of the host to generate an effective immune response. chemokines have a direct involvement in the pathogenesis of leishmaniasis, causing a rapid change in the expression of these molecules during infection by leishmania. | 2017 | 28767981 |
| leishmania infantum parasites subvert the host inflammatory response through the adenosine a2a receptor to promote the establishment of infection. | adenosine is an endogenously released purine nucleoside that signals through four widely expressed g protein-coupled receptors: a1, a2a, a2b, and a3. of these, a2ar is recognized as mediating major adenosine anti-inflammatory activity. during cutaneous leishmaniasis, adenosine induces immunosuppression, which promotes the establishment of infection. herein, we demonstrated that a2ar signaling is exploited by leishmania infantum parasites, the etiologic agent that causes visceral leishmaniasis, t ... | 2017 | 28775724 |
| cutaneous leishmaniasis by leishmania infantum: behind granulomatous lesions of unknown aetiology. | cutaneous leishmaniasis (cl) is underestimated in spain as in other european countries due to the polymorphism of its clinical manifestations and histopathological features discouraging doctors from suspecting leishmaniasis. mucosal manifestations (ml) are misdiagnosed due to the fact that they often mimic cancer. | 2017 | 28776770 |
| anti-leishmania activity of new ruthenium(ii) complexes: effect on parasite-host interaction. | leishmaniasis is a parasitic disease caused by protozoa of the genus leishmania. the many complications presented by the current treatment - including high toxicity, high cost and parasite resistance - make the development of new therapeutic agents indispensable. the present study aims to evaluate the anti-leishmania potential of new ruthenium(ii) complexes, cis‑[ru(ii)(η(2)-o2cr)(dppm)2]pf6, with dppm=bis(diphenylphosphino)methane and r=4-butylbenzoate (bbato) 1, 4-(methylthio)benzoate (mtbato) ... | 2017 | 28783554 |
| a misleading anamnesis: learning to suspect. | leishmaniasis represents a complex, globally widespread opportunistic infection ranging from the visceral form, also called kala-azar, to the mucocutaneous and cutaneous disease. it is endemic in the mediterranian basin, leishmania infantum being demonstrated as the main causative agent of autochthonous cases in sicily, italy. the long-term use of systemic antipsoriatic agents, including biotechnological drugs, may cause a higher susceptibility to opportunistic infections, so physicians maintain ... | 2017 | 28785336 |
| in vitro antileishmanial activity and iron superoxide dismutase inhibition of arylamine mannich base derivatives. | leishmaniasis is one of the world's most neglected diseases, and it has a worldwide prevalence of 12 million. there are no effective human vaccines for its prevention, and treatment is hampered by outdated drugs. therefore, research aiming at the development of new therapeutic tools to fight leishmaniasis remains a crucial goal today. with this purpose in mind, we present 20 arylaminoketone derivatives with a very interesting in vitro and in vivo efficacy against trypanosoma cruzi that have now ... | 2017 | 28789716 |
| sensitive molecular diagnostics for cutaneous leishmaniasis. | rapid diagnosis of cutaneous leishmaniasis (cl) and identification of leishmania species is highly important for the disease management. in israel, cl is caused mainly by leishmania major and leishmania tropica species. | 2017 | 28795077 |
| molecular detection of wolbachia pipientis in natural populations of sandfly vectors of leishmania infantum in endemic areas: first detection in lutzomyia longipalpis. | a polymerase chain reaction-based method was used to screen sandflies for infection with wolbachia (rickettsiales: rickettsiaceae), an intracellular bacterial endosymbiont found in many arthropods and filarial hosts. positive results were obtained in five of 200 field-collected sandflies and were confirmed by sequencing. all sandflies were lutzomyia longipalpis (diptera: psychodidae) captured in a region endemic for visceral leishmaniasis in brazil. this is the first study to identify wolbachia ... | 2017 | 28799248 |
| optimized transitory ectopic expression of promastigote surface antigen protein in nicotiana benthamiana, a potential anti-leishmaniasis vaccine candidate. | in recent years, plants have been shown to be an efficient alternative expression system for high-value pharmaceuticals such as vaccines. however, constitutive expression of recombinant protein remains uncertain on their level of production and biological activity. to overcome these problems, transitory expression systems have been developed. here, a series of experiments were performed to determine the most effective conditions to enhance vaccine antigen transient accumulation in nicotiana bent ... | 2017 | 28803053 |
| association between mast cells, tissue remodelation and parasite burden in the skin of dogs with visceral leishmaniasis. | canine visceral leishmaniosis (cvl) is a zoonosis of major public health impact caused by organisms of the genus leishmania which is transmitted to human and animals by phlebotomine sand flies. the skin is the first point of contact with leishmania parasites for sandy fly vectors and it is considered an important reservoir compartment in infected dogs. the aim of this study was to determine the main histophatologic alterations in ear skin of dogs naturally infected by leishmania infantum with di ... | 2017 | 28807304 |
| application of a specific quantitative real-time pcr (qpcr) to identify leishmania infantum dna in spleen, skin and hair samples of wild leporidae. | the aim of this study was to compare a quantitative real-time pcr (qpcr) validated for the detection of leishmania infantum in dogs with a nested pcr but in wild leporidae. additionally, l. infantum results from indirect immunofluorescent antibody test (ifat) and in vitro culture were also compared with qpcr. different samples (spleen, skin and hair) recovered from 224 european rabbits and 70 iberian hares from two green areas of madrid council were analysed for the detection of l. infantum. the ... | 2017 | 28807318 |
| main lesions in the central nervous system of dogs due to leishmania infantum infection. | canine visceral leishmaniasis (cvl) is endemic in são luís maranhão/brazil and it leads a varied clinical picture, including neurological signs. | 2017 | 28821261 |
| chemical composition of mentha pulegium and rosmarinus officinalis essential oils and their antileishmanial, antibacterial and antioxidant activities. | the aim of the study was the determination of the chemical composition of mentha pulegium l. and rosmarinus officinalis l. essential oils and the evaluation of their antileishmanial, antibacterial and antioxidant activities. essential oils (eos) were isolated using steam distillation and the chemical composition was determined using gc-ms analysis. the antibacterial activity was tested against ten pathogenic strains using the diffusion method, the minimum inhibitory concentration (mic) and the m ... | 2017 | 28821401 |
| leishmania infection in bats from a non-endemic region of leishmaniasis in brazil. | leishmaniasis is a complex of zoonotic diseases caused by parasites of the genus leishmania, which can develop in domestic as well as wild animals and humans throughout the world. currently, this disease is spreading in rural and urban areas of non-endemic regions in brazil. recently, bats have gained epidemiological significance in leishmaniasis due to its close relationship with human settlements. in this study, we investigated the presence of leishmania spp. dna in blood samples from 448 bats ... | 2017 | 28831941 |
| leishmania infection and blood food sources of phlebotomines in an area of brazil endemic for visceral and tegumentary leishmaniasis. | the aims of the study were to determine the blood feeding preferences of sandflies and to identify species of leishmania that infected phlebotomines in caxias, maranhão, brazil, an area that is highly endemic for leishmaniasis. sandflies were captured in light traps located in the peridomiciliary environments of randomly selected houses in urban and rural settings between 1800 and 0600 hours on new moon days between march 2013 and february 2015. dna extracts from 982 engorged female sandflies we ... | 2017 | 28837565 |
| structural and evolutionary analyses of leishmania alba proteins. | the alba superfamily proteins share a common rna-binding domain. these proteins participate in a variety of regulatory pathways by controlling developmental gene expression. they also interact with ribosomal subunits, translation factors, and other rna-binding proteins. the leishmania infantum genome encodes two alba-domain proteins, lialba1 and lialba3. in this work, we used homology modeling, protein-protein docking, and molecular dynamics (md) simulations to explore the details of the alba1-a ... | 2017 | 28847609 |
| the use of herbs against neglected diseases: evaluation of in vitro leishmanicidal and trypanocidal activity of stryphnodendron rotundifolium mart. | the evaluation of the leishmanicidal and trypanocidal activity of the hydroalcoholic extract of the bark of stryphnodendron rotundifolium mart. (ehcsr) was carried out to find an alternative treatment for parasitic diseases. ehcsr was prepared and used at four different concentrations (1000, 500, 250, 125 μg/ml) in in vitro assays for activity against leishmania promastigotes using the species leishmania brasiliensis and leishmania infantum and for trypanocidal activity using the epimastigotes o ... | 2017 | 28855804 |